The effect of antibiotic exposure on eicosanoid generation from arachidonic acid and gene expression in a primitive chordate,Branchiostoma belcheri

Chloramphenicol (Chl) is an effective antimicrobial agent widely used in veterinary medicine and commonly used in fish. Its use is restricted in the clinic because of adverse effects on the immune system and oxidative stress in mammals. However, the effects of Chl treatment on invertebrates remain u...
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Autor*in:	Dongjuan Yuan [verfasserIn] Minming Pan [verfasserIn] Qiuqiong Zou [verfasserIn] Chengyong Chen [verfasserIn] Shangwu Chen [verfasserIn] Anlong Xu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	Amphioxus Chloramphenicol Ampicillin Immune system Eicosanoid

Übergeordnetes Werk:	In: FEBS Open Bio - Wiley, 2013, 5(2015), 1, Seite 615-624
Übergeordnetes Werk:	volume:5 ; year:2015 ; number:1 ; pages:615-624

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.fob.2015.07.004

Katalog-ID:	DOAJ008899681

Internformat


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520			\|a Chloramphenicol (Chl) is an effective antimicrobial agent widely used in veterinary medicine and commonly used in fish. Its use is restricted in the clinic because of adverse effects on the immune system and oxidative stress in mammals. However, the effects of Chl treatment on invertebrates remain unclear. Amphioxus, a basal chordate, is an ideal model to study the origin and evolution of the vertebrate immune system as it has a primary vertebrate‐like arachidonic acid (AA) metabolic system. Here, we combined transcriptomic and lipidomic approaches to investigate the immune system and observe the oxygenated metabolites of AA to address the antibiotic effects on amphioxus. Tissue necrosis of the gill slits occurred in the Chl‐treated amphioxus, but fewer epithelial cells were lost when treated with both Chl and ampicillin (Amp). The immune related pathways were dysregulated in both of the antibiotic treatment groups. The Chl alone treatment resulted in immunosuppression with down‐regulation of the innate immune genes. In contrast, the Chl + Amp treatment resulted in immunostimulation to some extent, as shown by KEGG clustering. Furthermore, Chl induced a 3‐fold reduction in the level of the eicosanoids, while the Chl + Amp treatment resulted in 1.7‐fold increase of eicosanoid level. Thus in amphioxus, Amp might relieve the effects of the Chl‐induced immune suppression and increase the level of eicosanoids from AA. Finally, the oxygenated metabolites from AA might be crucial to evaluate the effects of Chl treatment in animals.
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700	0		\|a Shangwu Chen \|e verfasserin \|4 aut
700	0		\|a Anlong Xu \|e verfasserin \|4 aut
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912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_95
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
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912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
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912			\|a GBV_ILN_2068
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912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
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912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
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allfields	10.1016/j.fob.2015.07.004 doi (DE-627)DOAJ008899681 (DE-599)DOAJ49eac4bd15a94e0a89aa4133651bd714 DE-627 ger DE-627 rakwb eng QH301-705.5 Dongjuan Yuan verfasserin aut The effect of antibiotic exposure on eicosanoid generation from arachidonic acid and gene expression in a primitive chordate,Branchiostoma belcheri 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chloramphenicol (Chl) is an effective antimicrobial agent widely used in veterinary medicine and commonly used in fish. Its use is restricted in the clinic because of adverse effects on the immune system and oxidative stress in mammals. However, the effects of Chl treatment on invertebrates remain unclear. Amphioxus, a basal chordate, is an ideal model to study the origin and evolution of the vertebrate immune system as it has a primary vertebrate‐like arachidonic acid (AA) metabolic system. Here, we combined transcriptomic and lipidomic approaches to investigate the immune system and observe the oxygenated metabolites of AA to address the antibiotic effects on amphioxus. Tissue necrosis of the gill slits occurred in the Chl‐treated amphioxus, but fewer epithelial cells were lost when treated with both Chl and ampicillin (Amp). The immune related pathways were dysregulated in both of the antibiotic treatment groups. The Chl alone treatment resulted in immunosuppression with down‐regulation of the innate immune genes. In contrast, the Chl + Amp treatment resulted in immunostimulation to some extent, as shown by KEGG clustering. Furthermore, Chl induced a 3‐fold reduction in the level of the eicosanoids, while the Chl + Amp treatment resulted in 1.7‐fold increase of eicosanoid level. Thus in amphioxus, Amp might relieve the effects of the Chl‐induced immune suppression and increase the level of eicosanoids from AA. Finally, the oxygenated metabolites from AA might be crucial to evaluate the effects of Chl treatment in animals. Amphioxus Chloramphenicol Ampicillin Immune system Eicosanoid Biology (General) Minming Pan verfasserin aut Qiuqiong Zou verfasserin aut Chengyong Chen verfasserin aut Shangwu Chen verfasserin aut Anlong Xu verfasserin aut In FEBS Open Bio Wiley, 2013 5(2015), 1, Seite 615-624 (DE-627)686948351 (DE-600)2651702-4 22115463 nnns volume:5 year:2015 number:1 pages:615-624 https://doi.org/10.1016/j.fob.2015.07.004 kostenfrei https://doaj.org/article/49eac4bd15a94e0a89aa4133651bd714 kostenfrei https://doi.org/10.1016/j.fob.2015.07.004 kostenfrei https://doaj.org/toc/2211-5463 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2015 1 615-624
spelling	10.1016/j.fob.2015.07.004 doi (DE-627)DOAJ008899681 (DE-599)DOAJ49eac4bd15a94e0a89aa4133651bd714 DE-627 ger DE-627 rakwb eng QH301-705.5 Dongjuan Yuan verfasserin aut The effect of antibiotic exposure on eicosanoid generation from arachidonic acid and gene expression in a primitive chordate,Branchiostoma belcheri 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chloramphenicol (Chl) is an effective antimicrobial agent widely used in veterinary medicine and commonly used in fish. Its use is restricted in the clinic because of adverse effects on the immune system and oxidative stress in mammals. However, the effects of Chl treatment on invertebrates remain unclear. Amphioxus, a basal chordate, is an ideal model to study the origin and evolution of the vertebrate immune system as it has a primary vertebrate‐like arachidonic acid (AA) metabolic system. Here, we combined transcriptomic and lipidomic approaches to investigate the immune system and observe the oxygenated metabolites of AA to address the antibiotic effects on amphioxus. Tissue necrosis of the gill slits occurred in the Chl‐treated amphioxus, but fewer epithelial cells were lost when treated with both Chl and ampicillin (Amp). The immune related pathways were dysregulated in both of the antibiotic treatment groups. The Chl alone treatment resulted in immunosuppression with down‐regulation of the innate immune genes. In contrast, the Chl + Amp treatment resulted in immunostimulation to some extent, as shown by KEGG clustering. Furthermore, Chl induced a 3‐fold reduction in the level of the eicosanoids, while the Chl + Amp treatment resulted in 1.7‐fold increase of eicosanoid level. Thus in amphioxus, Amp might relieve the effects of the Chl‐induced immune suppression and increase the level of eicosanoids from AA. Finally, the oxygenated metabolites from AA might be crucial to evaluate the effects of Chl treatment in animals. Amphioxus Chloramphenicol Ampicillin Immune system Eicosanoid Biology (General) Minming Pan verfasserin aut Qiuqiong Zou verfasserin aut Chengyong Chen verfasserin aut Shangwu Chen verfasserin aut Anlong Xu verfasserin aut In FEBS Open Bio Wiley, 2013 5(2015), 1, Seite 615-624 (DE-627)686948351 (DE-600)2651702-4 22115463 nnns volume:5 year:2015 number:1 pages:615-624 https://doi.org/10.1016/j.fob.2015.07.004 kostenfrei https://doaj.org/article/49eac4bd15a94e0a89aa4133651bd714 kostenfrei https://doi.org/10.1016/j.fob.2015.07.004 kostenfrei https://doaj.org/toc/2211-5463 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2015 1 615-624
allfields_unstemmed	10.1016/j.fob.2015.07.004 doi (DE-627)DOAJ008899681 (DE-599)DOAJ49eac4bd15a94e0a89aa4133651bd714 DE-627 ger DE-627 rakwb eng QH301-705.5 Dongjuan Yuan verfasserin aut The effect of antibiotic exposure on eicosanoid generation from arachidonic acid and gene expression in a primitive chordate,Branchiostoma belcheri 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chloramphenicol (Chl) is an effective antimicrobial agent widely used in veterinary medicine and commonly used in fish. Its use is restricted in the clinic because of adverse effects on the immune system and oxidative stress in mammals. However, the effects of Chl treatment on invertebrates remain unclear. Amphioxus, a basal chordate, is an ideal model to study the origin and evolution of the vertebrate immune system as it has a primary vertebrate‐like arachidonic acid (AA) metabolic system. Here, we combined transcriptomic and lipidomic approaches to investigate the immune system and observe the oxygenated metabolites of AA to address the antibiotic effects on amphioxus. Tissue necrosis of the gill slits occurred in the Chl‐treated amphioxus, but fewer epithelial cells were lost when treated with both Chl and ampicillin (Amp). The immune related pathways were dysregulated in both of the antibiotic treatment groups. The Chl alone treatment resulted in immunosuppression with down‐regulation of the innate immune genes. In contrast, the Chl + Amp treatment resulted in immunostimulation to some extent, as shown by KEGG clustering. Furthermore, Chl induced a 3‐fold reduction in the level of the eicosanoids, while the Chl + Amp treatment resulted in 1.7‐fold increase of eicosanoid level. Thus in amphioxus, Amp might relieve the effects of the Chl‐induced immune suppression and increase the level of eicosanoids from AA. Finally, the oxygenated metabolites from AA might be crucial to evaluate the effects of Chl treatment in animals. Amphioxus Chloramphenicol Ampicillin Immune system Eicosanoid Biology (General) Minming Pan verfasserin aut Qiuqiong Zou verfasserin aut Chengyong Chen verfasserin aut Shangwu Chen verfasserin aut Anlong Xu verfasserin aut In FEBS Open Bio Wiley, 2013 5(2015), 1, Seite 615-624 (DE-627)686948351 (DE-600)2651702-4 22115463 nnns volume:5 year:2015 number:1 pages:615-624 https://doi.org/10.1016/j.fob.2015.07.004 kostenfrei https://doaj.org/article/49eac4bd15a94e0a89aa4133651bd714 kostenfrei https://doi.org/10.1016/j.fob.2015.07.004 kostenfrei https://doaj.org/toc/2211-5463 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2015 1 615-624
allfieldsGer	10.1016/j.fob.2015.07.004 doi (DE-627)DOAJ008899681 (DE-599)DOAJ49eac4bd15a94e0a89aa4133651bd714 DE-627 ger DE-627 rakwb eng QH301-705.5 Dongjuan Yuan verfasserin aut The effect of antibiotic exposure on eicosanoid generation from arachidonic acid and gene expression in a primitive chordate,Branchiostoma belcheri 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chloramphenicol (Chl) is an effective antimicrobial agent widely used in veterinary medicine and commonly used in fish. Its use is restricted in the clinic because of adverse effects on the immune system and oxidative stress in mammals. However, the effects of Chl treatment on invertebrates remain unclear. Amphioxus, a basal chordate, is an ideal model to study the origin and evolution of the vertebrate immune system as it has a primary vertebrate‐like arachidonic acid (AA) metabolic system. Here, we combined transcriptomic and lipidomic approaches to investigate the immune system and observe the oxygenated metabolites of AA to address the antibiotic effects on amphioxus. Tissue necrosis of the gill slits occurred in the Chl‐treated amphioxus, but fewer epithelial cells were lost when treated with both Chl and ampicillin (Amp). The immune related pathways were dysregulated in both of the antibiotic treatment groups. The Chl alone treatment resulted in immunosuppression with down‐regulation of the innate immune genes. In contrast, the Chl + Amp treatment resulted in immunostimulation to some extent, as shown by KEGG clustering. Furthermore, Chl induced a 3‐fold reduction in the level of the eicosanoids, while the Chl + Amp treatment resulted in 1.7‐fold increase of eicosanoid level. Thus in amphioxus, Amp might relieve the effects of the Chl‐induced immune suppression and increase the level of eicosanoids from AA. Finally, the oxygenated metabolites from AA might be crucial to evaluate the effects of Chl treatment in animals. Amphioxus Chloramphenicol Ampicillin Immune system Eicosanoid Biology (General) Minming Pan verfasserin aut Qiuqiong Zou verfasserin aut Chengyong Chen verfasserin aut Shangwu Chen verfasserin aut Anlong Xu verfasserin aut In FEBS Open Bio Wiley, 2013 5(2015), 1, Seite 615-624 (DE-627)686948351 (DE-600)2651702-4 22115463 nnns volume:5 year:2015 number:1 pages:615-624 https://doi.org/10.1016/j.fob.2015.07.004 kostenfrei https://doaj.org/article/49eac4bd15a94e0a89aa4133651bd714 kostenfrei https://doi.org/10.1016/j.fob.2015.07.004 kostenfrei https://doaj.org/toc/2211-5463 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2015 1 615-624
allfieldsSound	10.1016/j.fob.2015.07.004 doi (DE-627)DOAJ008899681 (DE-599)DOAJ49eac4bd15a94e0a89aa4133651bd714 DE-627 ger DE-627 rakwb eng QH301-705.5 Dongjuan Yuan verfasserin aut The effect of antibiotic exposure on eicosanoid generation from arachidonic acid and gene expression in a primitive chordate,Branchiostoma belcheri 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chloramphenicol (Chl) is an effective antimicrobial agent widely used in veterinary medicine and commonly used in fish. Its use is restricted in the clinic because of adverse effects on the immune system and oxidative stress in mammals. However, the effects of Chl treatment on invertebrates remain unclear. Amphioxus, a basal chordate, is an ideal model to study the origin and evolution of the vertebrate immune system as it has a primary vertebrate‐like arachidonic acid (AA) metabolic system. Here, we combined transcriptomic and lipidomic approaches to investigate the immune system and observe the oxygenated metabolites of AA to address the antibiotic effects on amphioxus. Tissue necrosis of the gill slits occurred in the Chl‐treated amphioxus, but fewer epithelial cells were lost when treated with both Chl and ampicillin (Amp). The immune related pathways were dysregulated in both of the antibiotic treatment groups. The Chl alone treatment resulted in immunosuppression with down‐regulation of the innate immune genes. In contrast, the Chl + Amp treatment resulted in immunostimulation to some extent, as shown by KEGG clustering. Furthermore, Chl induced a 3‐fold reduction in the level of the eicosanoids, while the Chl + Amp treatment resulted in 1.7‐fold increase of eicosanoid level. Thus in amphioxus, Amp might relieve the effects of the Chl‐induced immune suppression and increase the level of eicosanoids from AA. Finally, the oxygenated metabolites from AA might be crucial to evaluate the effects of Chl treatment in animals. Amphioxus Chloramphenicol Ampicillin Immune system Eicosanoid Biology (General) Minming Pan verfasserin aut Qiuqiong Zou verfasserin aut Chengyong Chen verfasserin aut Shangwu Chen verfasserin aut Anlong Xu verfasserin aut In FEBS Open Bio Wiley, 2013 5(2015), 1, Seite 615-624 (DE-627)686948351 (DE-600)2651702-4 22115463 nnns volume:5 year:2015 number:1 pages:615-624 https://doi.org/10.1016/j.fob.2015.07.004 kostenfrei https://doaj.org/article/49eac4bd15a94e0a89aa4133651bd714 kostenfrei https://doi.org/10.1016/j.fob.2015.07.004 kostenfrei https://doaj.org/toc/2211-5463 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2015 1 615-624
language	English
source	In FEBS Open Bio 5(2015), 1, Seite 615-624 volume:5 year:2015 number:1 pages:615-624
sourceStr	In FEBS Open Bio 5(2015), 1, Seite 615-624 volume:5 year:2015 number:1 pages:615-624
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Amphioxus Chloramphenicol Ampicillin Immune system Eicosanoid Biology (General)
isfreeaccess_bool	true
container_title	FEBS Open Bio
authorswithroles_txt_mv	Dongjuan Yuan @@aut@@ Minming Pan @@aut@@ Qiuqiong Zou @@aut@@ Chengyong Chen @@aut@@ Shangwu Chen @@aut@@ Anlong Xu @@aut@@
publishDateDaySort_date	2015-01-01T00:00:00Z
hierarchy_top_id	686948351
id	DOAJ008899681
language_de	englisch
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author	Dongjuan Yuan
spellingShingle	Dongjuan Yuan misc QH301-705.5 misc Amphioxus misc Chloramphenicol misc Ampicillin misc Immune system misc Eicosanoid misc Biology (General) The effect of antibiotic exposure on eicosanoid generation from arachidonic acid and gene expression in a primitive chordate,Branchiostoma belcheri
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topic_title	QH301-705.5 The effect of antibiotic exposure on eicosanoid generation from arachidonic acid and gene expression in a primitive chordate,Branchiostoma belcheri Amphioxus Chloramphenicol Ampicillin Immune system Eicosanoid
topic	misc QH301-705.5 misc Amphioxus misc Chloramphenicol misc Ampicillin misc Immune system misc Eicosanoid misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc Amphioxus misc Chloramphenicol misc Ampicillin misc Immune system misc Eicosanoid misc Biology (General)
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title	The effect of antibiotic exposure on eicosanoid generation from arachidonic acid and gene expression in a primitive chordate,Branchiostoma belcheri
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title_full	The effect of antibiotic exposure on eicosanoid generation from arachidonic acid and gene expression in a primitive chordate,Branchiostoma belcheri
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title_sort	effect of antibiotic exposure on eicosanoid generation from arachidonic acid and gene expression in a primitive chordate,branchiostoma belcheri
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title_auth	The effect of antibiotic exposure on eicosanoid generation from arachidonic acid and gene expression in a primitive chordate,Branchiostoma belcheri
abstract	Chloramphenicol (Chl) is an effective antimicrobial agent widely used in veterinary medicine and commonly used in fish. Its use is restricted in the clinic because of adverse effects on the immune system and oxidative stress in mammals. However, the effects of Chl treatment on invertebrates remain unclear. Amphioxus, a basal chordate, is an ideal model to study the origin and evolution of the vertebrate immune system as it has a primary vertebrate‐like arachidonic acid (AA) metabolic system. Here, we combined transcriptomic and lipidomic approaches to investigate the immune system and observe the oxygenated metabolites of AA to address the antibiotic effects on amphioxus. Tissue necrosis of the gill slits occurred in the Chl‐treated amphioxus, but fewer epithelial cells were lost when treated with both Chl and ampicillin (Amp). The immune related pathways were dysregulated in both of the antibiotic treatment groups. The Chl alone treatment resulted in immunosuppression with down‐regulation of the innate immune genes. In contrast, the Chl + Amp treatment resulted in immunostimulation to some extent, as shown by KEGG clustering. Furthermore, Chl induced a 3‐fold reduction in the level of the eicosanoids, while the Chl + Amp treatment resulted in 1.7‐fold increase of eicosanoid level. Thus in amphioxus, Amp might relieve the effects of the Chl‐induced immune suppression and increase the level of eicosanoids from AA. Finally, the oxygenated metabolites from AA might be crucial to evaluate the effects of Chl treatment in animals.
abstractGer	Chloramphenicol (Chl) is an effective antimicrobial agent widely used in veterinary medicine and commonly used in fish. Its use is restricted in the clinic because of adverse effects on the immune system and oxidative stress in mammals. However, the effects of Chl treatment on invertebrates remain unclear. Amphioxus, a basal chordate, is an ideal model to study the origin and evolution of the vertebrate immune system as it has a primary vertebrate‐like arachidonic acid (AA) metabolic system. Here, we combined transcriptomic and lipidomic approaches to investigate the immune system and observe the oxygenated metabolites of AA to address the antibiotic effects on amphioxus. Tissue necrosis of the gill slits occurred in the Chl‐treated amphioxus, but fewer epithelial cells were lost when treated with both Chl and ampicillin (Amp). The immune related pathways were dysregulated in both of the antibiotic treatment groups. The Chl alone treatment resulted in immunosuppression with down‐regulation of the innate immune genes. In contrast, the Chl + Amp treatment resulted in immunostimulation to some extent, as shown by KEGG clustering. Furthermore, Chl induced a 3‐fold reduction in the level of the eicosanoids, while the Chl + Amp treatment resulted in 1.7‐fold increase of eicosanoid level. Thus in amphioxus, Amp might relieve the effects of the Chl‐induced immune suppression and increase the level of eicosanoids from AA. Finally, the oxygenated metabolites from AA might be crucial to evaluate the effects of Chl treatment in animals.
abstract_unstemmed	Chloramphenicol (Chl) is an effective antimicrobial agent widely used in veterinary medicine and commonly used in fish. Its use is restricted in the clinic because of adverse effects on the immune system and oxidative stress in mammals. However, the effects of Chl treatment on invertebrates remain unclear. Amphioxus, a basal chordate, is an ideal model to study the origin and evolution of the vertebrate immune system as it has a primary vertebrate‐like arachidonic acid (AA) metabolic system. Here, we combined transcriptomic and lipidomic approaches to investigate the immune system and observe the oxygenated metabolites of AA to address the antibiotic effects on amphioxus. Tissue necrosis of the gill slits occurred in the Chl‐treated amphioxus, but fewer epithelial cells were lost when treated with both Chl and ampicillin (Amp). The immune related pathways were dysregulated in both of the antibiotic treatment groups. The Chl alone treatment resulted in immunosuppression with down‐regulation of the innate immune genes. In contrast, the Chl + Amp treatment resulted in immunostimulation to some extent, as shown by KEGG clustering. Furthermore, Chl induced a 3‐fold reduction in the level of the eicosanoids, while the Chl + Amp treatment resulted in 1.7‐fold increase of eicosanoid level. Thus in amphioxus, Amp might relieve the effects of the Chl‐induced immune suppression and increase the level of eicosanoids from AA. Finally, the oxygenated metabolites from AA might be crucial to evaluate the effects of Chl treatment in animals.
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title_short	The effect of antibiotic exposure on eicosanoid generation from arachidonic acid and gene expression in a primitive chordate,Branchiostoma belcheri
url	https://doi.org/10.1016/j.fob.2015.07.004 https://doaj.org/article/49eac4bd15a94e0a89aa4133651bd714 https://doaj.org/toc/2211-5463
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Its use is restricted in the clinic because of adverse effects on the immune system and oxidative stress in mammals. However, the effects of Chl treatment on invertebrates remain unclear. Amphioxus, a basal chordate, is an ideal model to study the origin and evolution of the vertebrate immune system as it has a primary vertebrate‐like arachidonic acid (AA) metabolic system. Here, we combined transcriptomic and lipidomic approaches to investigate the immune system and observe the oxygenated metabolites of AA to address the antibiotic effects on amphioxus. Tissue necrosis of the gill slits occurred in the Chl‐treated amphioxus, but fewer epithelial cells were lost when treated with both Chl and ampicillin (Amp). The immune related pathways were dysregulated in both of the antibiotic treatment groups. The Chl alone treatment resulted in immunosuppression with down‐regulation of the innate immune genes. In contrast, the Chl + Amp treatment resulted in immunostimulation to some extent, as shown by KEGG clustering. Furthermore, Chl induced a 3‐fold reduction in the level of the eicosanoids, while the Chl + Amp treatment resulted in 1.7‐fold increase of eicosanoid level. Thus in amphioxus, Amp might relieve the effects of the Chl‐induced immune suppression and increase the level of eicosanoids from AA. 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The effect of antibiotic exposure on eicosanoid generation from arachidonic acid and gene expression in a primitive chordate,Branchiostoma belcheri

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