FIRST RESULTS ON THE PRESENCE AND THE MOLECULAR CHARACTERIZATION OF ANISAKID NEMATODES IN MARINE FISH CAUGHT OFF NORTHERN SARDINIA

Anisakidosis is a parasitic zoonosis caused by nematodes of the family Anisakidae, belonging to the genera Anisakis, Contracaecum and Pseudoterranova. Molecular studies have shown that Anisakis larvae comprise a number of sibling species, which have different genetic structures, hosts and geographic...
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Autor*in:	T. Tedde [verfasserIn] M.C. Piras [verfasserIn] C. Pinna [verfasserIn] S. Virgilio [verfasserIn] G. Terrosu [verfasserIn] A. Piras [verfasserIn] G. Garippa [verfasserIn] P. Merella [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; Italienisch

Erschienen:	2011

Schlagwörter:	parasitic zoonosis, commercial fish, Anisakis pegreffii, Anisakis physeteris, PCRRFLP

Übergeordnetes Werk:	In: Italian Journal of Food Safety - PAGEPress Publications, 2013, 1(2011), 1zero, Seite 185-190
Übergeordnetes Werk:	volume:1 ; year:2011 ; number:1zero ; pages:185-190

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.4081/ijfs.2011.1S.185

Katalog-ID:	DOAJ008935378

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allfields	10.4081/ijfs.2011.1S.185 doi (DE-627)DOAJ008935378 (DE-599)DOAJc00b36f23d464f7985b52011f7ae57b6 DE-627 ger DE-627 rakwb eng ita TP368-456 T. Tedde verfasserin aut FIRST RESULTS ON THE PRESENCE AND THE MOLECULAR CHARACTERIZATION OF ANISAKID NEMATODES IN MARINE FISH CAUGHT OFF NORTHERN SARDINIA 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Anisakidosis is a parasitic zoonosis caused by nematodes of the family Anisakidae, belonging to the genera Anisakis, Contracaecum and Pseudoterranova. Molecular studies have shown that Anisakis larvae comprise a number of sibling species, which have different genetic structures, hosts and geographical distribution. A great variety of fish species can harbour infectious third stage larvae of this nematode. The preliminary results of a study carried out to evaluate the occurrence of this parasite in commercial fish caught off northern Sardinia are herein reported. From October 2008 to November 2009, 599 specimens of 8 commercial fish species were examined for anisakid larvae through visual inspection of body cavity and peptic digestion of the muscle. Isolated Anisakis sp. larvae were observed at light microscope and identified as Type I or Type II (sensu Berland, 1961). Out of 599 fish examined, 239 (40%) were infected by 1187 anisakid larvae, belonging to the genera Anisakis (1169 Type I and 18 Type II) and Hysterothylacium (692). The molecular identification of Anisakis spp. was carried out on a subsample of 30% of Type I larvae and all Type II larvae. Specimens were firstly examined using a species-specific PCR, with primers designed for Anisakis pegreffii (APEF) and Anisakis physeteris (APHF), and ITS-2 of nuclear rDNA. The results were confirmed by the analysis of the ITS region of nuclear rDNA (ITS-1, 5.8S and ITS-2) using the restriction enzymes HinfI and HhaI in PCR-RFLP. Type I larvae examined were all identified as A. pegreffii, and Type II were all A. physeteris. This is the first contribution to the epidemiology and molecular characterization of Anisakis spp. in commercial fish caught off Sardinia. parasitic zoonosis, commercial fish, Anisakis pegreffii, Anisakis physeteris, PCRRFLP Food processing and manufacture M.C. Piras verfasserin aut C. Pinna verfasserin aut S. Virgilio verfasserin aut G. Terrosu verfasserin aut A. Piras verfasserin aut G. Garippa verfasserin aut P. Merella verfasserin aut In Italian Journal of Food Safety PAGEPress Publications, 2013 1(2011), 1zero, Seite 185-190 (DE-627)74650697X (DE-600)2717104-8 22397132 nnns volume:1 year:2011 number:1zero pages:185-190 https://doi.org/10.4081/ijfs.2011.1S.185 kostenfrei https://doaj.org/article/c00b36f23d464f7985b52011f7ae57b6 kostenfrei http://www.pagepressjournals.org/index.php/ijfs/article/view/861 kostenfrei https://doaj.org/toc/2239-7132 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 1 2011 1zero 185-190
spelling	10.4081/ijfs.2011.1S.185 doi (DE-627)DOAJ008935378 (DE-599)DOAJc00b36f23d464f7985b52011f7ae57b6 DE-627 ger DE-627 rakwb eng ita TP368-456 T. Tedde verfasserin aut FIRST RESULTS ON THE PRESENCE AND THE MOLECULAR CHARACTERIZATION OF ANISAKID NEMATODES IN MARINE FISH CAUGHT OFF NORTHERN SARDINIA 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Anisakidosis is a parasitic zoonosis caused by nematodes of the family Anisakidae, belonging to the genera Anisakis, Contracaecum and Pseudoterranova. Molecular studies have shown that Anisakis larvae comprise a number of sibling species, which have different genetic structures, hosts and geographical distribution. A great variety of fish species can harbour infectious third stage larvae of this nematode. The preliminary results of a study carried out to evaluate the occurrence of this parasite in commercial fish caught off northern Sardinia are herein reported. From October 2008 to November 2009, 599 specimens of 8 commercial fish species were examined for anisakid larvae through visual inspection of body cavity and peptic digestion of the muscle. Isolated Anisakis sp. larvae were observed at light microscope and identified as Type I or Type II (sensu Berland, 1961). Out of 599 fish examined, 239 (40%) were infected by 1187 anisakid larvae, belonging to the genera Anisakis (1169 Type I and 18 Type II) and Hysterothylacium (692). The molecular identification of Anisakis spp. was carried out on a subsample of 30% of Type I larvae and all Type II larvae. Specimens were firstly examined using a species-specific PCR, with primers designed for Anisakis pegreffii (APEF) and Anisakis physeteris (APHF), and ITS-2 of nuclear rDNA. The results were confirmed by the analysis of the ITS region of nuclear rDNA (ITS-1, 5.8S and ITS-2) using the restriction enzymes HinfI and HhaI in PCR-RFLP. Type I larvae examined were all identified as A. pegreffii, and Type II were all A. physeteris. This is the first contribution to the epidemiology and molecular characterization of Anisakis spp. in commercial fish caught off Sardinia. parasitic zoonosis, commercial fish, Anisakis pegreffii, Anisakis physeteris, PCRRFLP Food processing and manufacture M.C. Piras verfasserin aut C. Pinna verfasserin aut S. Virgilio verfasserin aut G. Terrosu verfasserin aut A. Piras verfasserin aut G. Garippa verfasserin aut P. Merella verfasserin aut In Italian Journal of Food Safety PAGEPress Publications, 2013 1(2011), 1zero, Seite 185-190 (DE-627)74650697X (DE-600)2717104-8 22397132 nnns volume:1 year:2011 number:1zero pages:185-190 https://doi.org/10.4081/ijfs.2011.1S.185 kostenfrei https://doaj.org/article/c00b36f23d464f7985b52011f7ae57b6 kostenfrei http://www.pagepressjournals.org/index.php/ijfs/article/view/861 kostenfrei https://doaj.org/toc/2239-7132 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 1 2011 1zero 185-190
allfields_unstemmed	10.4081/ijfs.2011.1S.185 doi (DE-627)DOAJ008935378 (DE-599)DOAJc00b36f23d464f7985b52011f7ae57b6 DE-627 ger DE-627 rakwb eng ita TP368-456 T. Tedde verfasserin aut FIRST RESULTS ON THE PRESENCE AND THE MOLECULAR CHARACTERIZATION OF ANISAKID NEMATODES IN MARINE FISH CAUGHT OFF NORTHERN SARDINIA 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Anisakidosis is a parasitic zoonosis caused by nematodes of the family Anisakidae, belonging to the genera Anisakis, Contracaecum and Pseudoterranova. Molecular studies have shown that Anisakis larvae comprise a number of sibling species, which have different genetic structures, hosts and geographical distribution. A great variety of fish species can harbour infectious third stage larvae of this nematode. The preliminary results of a study carried out to evaluate the occurrence of this parasite in commercial fish caught off northern Sardinia are herein reported. From October 2008 to November 2009, 599 specimens of 8 commercial fish species were examined for anisakid larvae through visual inspection of body cavity and peptic digestion of the muscle. Isolated Anisakis sp. larvae were observed at light microscope and identified as Type I or Type II (sensu Berland, 1961). Out of 599 fish examined, 239 (40%) were infected by 1187 anisakid larvae, belonging to the genera Anisakis (1169 Type I and 18 Type II) and Hysterothylacium (692). The molecular identification of Anisakis spp. was carried out on a subsample of 30% of Type I larvae and all Type II larvae. Specimens were firstly examined using a species-specific PCR, with primers designed for Anisakis pegreffii (APEF) and Anisakis physeteris (APHF), and ITS-2 of nuclear rDNA. The results were confirmed by the analysis of the ITS region of nuclear rDNA (ITS-1, 5.8S and ITS-2) using the restriction enzymes HinfI and HhaI in PCR-RFLP. Type I larvae examined were all identified as A. pegreffii, and Type II were all A. physeteris. This is the first contribution to the epidemiology and molecular characterization of Anisakis spp. in commercial fish caught off Sardinia. parasitic zoonosis, commercial fish, Anisakis pegreffii, Anisakis physeteris, PCRRFLP Food processing and manufacture M.C. Piras verfasserin aut C. Pinna verfasserin aut S. Virgilio verfasserin aut G. Terrosu verfasserin aut A. Piras verfasserin aut G. Garippa verfasserin aut P. Merella verfasserin aut In Italian Journal of Food Safety PAGEPress Publications, 2013 1(2011), 1zero, Seite 185-190 (DE-627)74650697X (DE-600)2717104-8 22397132 nnns volume:1 year:2011 number:1zero pages:185-190 https://doi.org/10.4081/ijfs.2011.1S.185 kostenfrei https://doaj.org/article/c00b36f23d464f7985b52011f7ae57b6 kostenfrei http://www.pagepressjournals.org/index.php/ijfs/article/view/861 kostenfrei https://doaj.org/toc/2239-7132 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 1 2011 1zero 185-190
allfieldsGer	10.4081/ijfs.2011.1S.185 doi (DE-627)DOAJ008935378 (DE-599)DOAJc00b36f23d464f7985b52011f7ae57b6 DE-627 ger DE-627 rakwb eng ita TP368-456 T. Tedde verfasserin aut FIRST RESULTS ON THE PRESENCE AND THE MOLECULAR CHARACTERIZATION OF ANISAKID NEMATODES IN MARINE FISH CAUGHT OFF NORTHERN SARDINIA 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Anisakidosis is a parasitic zoonosis caused by nematodes of the family Anisakidae, belonging to the genera Anisakis, Contracaecum and Pseudoterranova. Molecular studies have shown that Anisakis larvae comprise a number of sibling species, which have different genetic structures, hosts and geographical distribution. A great variety of fish species can harbour infectious third stage larvae of this nematode. The preliminary results of a study carried out to evaluate the occurrence of this parasite in commercial fish caught off northern Sardinia are herein reported. From October 2008 to November 2009, 599 specimens of 8 commercial fish species were examined for anisakid larvae through visual inspection of body cavity and peptic digestion of the muscle. Isolated Anisakis sp. larvae were observed at light microscope and identified as Type I or Type II (sensu Berland, 1961). Out of 599 fish examined, 239 (40%) were infected by 1187 anisakid larvae, belonging to the genera Anisakis (1169 Type I and 18 Type II) and Hysterothylacium (692). The molecular identification of Anisakis spp. was carried out on a subsample of 30% of Type I larvae and all Type II larvae. Specimens were firstly examined using a species-specific PCR, with primers designed for Anisakis pegreffii (APEF) and Anisakis physeteris (APHF), and ITS-2 of nuclear rDNA. The results were confirmed by the analysis of the ITS region of nuclear rDNA (ITS-1, 5.8S and ITS-2) using the restriction enzymes HinfI and HhaI in PCR-RFLP. Type I larvae examined were all identified as A. pegreffii, and Type II were all A. physeteris. This is the first contribution to the epidemiology and molecular characterization of Anisakis spp. in commercial fish caught off Sardinia. parasitic zoonosis, commercial fish, Anisakis pegreffii, Anisakis physeteris, PCRRFLP Food processing and manufacture M.C. Piras verfasserin aut C. Pinna verfasserin aut S. Virgilio verfasserin aut G. Terrosu verfasserin aut A. Piras verfasserin aut G. Garippa verfasserin aut P. Merella verfasserin aut In Italian Journal of Food Safety PAGEPress Publications, 2013 1(2011), 1zero, Seite 185-190 (DE-627)74650697X (DE-600)2717104-8 22397132 nnns volume:1 year:2011 number:1zero pages:185-190 https://doi.org/10.4081/ijfs.2011.1S.185 kostenfrei https://doaj.org/article/c00b36f23d464f7985b52011f7ae57b6 kostenfrei http://www.pagepressjournals.org/index.php/ijfs/article/view/861 kostenfrei https://doaj.org/toc/2239-7132 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 1 2011 1zero 185-190
allfieldsSound	10.4081/ijfs.2011.1S.185 doi (DE-627)DOAJ008935378 (DE-599)DOAJc00b36f23d464f7985b52011f7ae57b6 DE-627 ger DE-627 rakwb eng ita TP368-456 T. Tedde verfasserin aut FIRST RESULTS ON THE PRESENCE AND THE MOLECULAR CHARACTERIZATION OF ANISAKID NEMATODES IN MARINE FISH CAUGHT OFF NORTHERN SARDINIA 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Anisakidosis is a parasitic zoonosis caused by nematodes of the family Anisakidae, belonging to the genera Anisakis, Contracaecum and Pseudoterranova. Molecular studies have shown that Anisakis larvae comprise a number of sibling species, which have different genetic structures, hosts and geographical distribution. A great variety of fish species can harbour infectious third stage larvae of this nematode. The preliminary results of a study carried out to evaluate the occurrence of this parasite in commercial fish caught off northern Sardinia are herein reported. From October 2008 to November 2009, 599 specimens of 8 commercial fish species were examined for anisakid larvae through visual inspection of body cavity and peptic digestion of the muscle. Isolated Anisakis sp. larvae were observed at light microscope and identified as Type I or Type II (sensu Berland, 1961). Out of 599 fish examined, 239 (40%) were infected by 1187 anisakid larvae, belonging to the genera Anisakis (1169 Type I and 18 Type II) and Hysterothylacium (692). The molecular identification of Anisakis spp. was carried out on a subsample of 30% of Type I larvae and all Type II larvae. Specimens were firstly examined using a species-specific PCR, with primers designed for Anisakis pegreffii (APEF) and Anisakis physeteris (APHF), and ITS-2 of nuclear rDNA. The results were confirmed by the analysis of the ITS region of nuclear rDNA (ITS-1, 5.8S and ITS-2) using the restriction enzymes HinfI and HhaI in PCR-RFLP. Type I larvae examined were all identified as A. pegreffii, and Type II were all A. physeteris. This is the first contribution to the epidemiology and molecular characterization of Anisakis spp. in commercial fish caught off Sardinia. parasitic zoonosis, commercial fish, Anisakis pegreffii, Anisakis physeteris, PCRRFLP Food processing and manufacture M.C. Piras verfasserin aut C. Pinna verfasserin aut S. Virgilio verfasserin aut G. Terrosu verfasserin aut A. Piras verfasserin aut G. Garippa verfasserin aut P. Merella verfasserin aut In Italian Journal of Food Safety PAGEPress Publications, 2013 1(2011), 1zero, Seite 185-190 (DE-627)74650697X (DE-600)2717104-8 22397132 nnns volume:1 year:2011 number:1zero pages:185-190 https://doi.org/10.4081/ijfs.2011.1S.185 kostenfrei https://doaj.org/article/c00b36f23d464f7985b52011f7ae57b6 kostenfrei http://www.pagepressjournals.org/index.php/ijfs/article/view/861 kostenfrei https://doaj.org/toc/2239-7132 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 1 2011 1zero 185-190
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source	In Italian Journal of Food Safety 1(2011), 1zero, Seite 185-190 volume:1 year:2011 number:1zero pages:185-190
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author	T. Tedde
spellingShingle	T. Tedde misc TP368-456 misc parasitic zoonosis, commercial fish, Anisakis pegreffii, Anisakis physeteris, PCRRFLP misc Food processing and manufacture FIRST RESULTS ON THE PRESENCE AND THE MOLECULAR CHARACTERIZATION OF ANISAKID NEMATODES IN MARINE FISH CAUGHT OFF NORTHERN SARDINIA
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issn	22397132
topic_title	TP368-456 FIRST RESULTS ON THE PRESENCE AND THE MOLECULAR CHARACTERIZATION OF ANISAKID NEMATODES IN MARINE FISH CAUGHT OFF NORTHERN SARDINIA parasitic zoonosis, commercial fish, Anisakis pegreffii, Anisakis physeteris, PCRRFLP
topic	misc TP368-456 misc parasitic zoonosis, commercial fish, Anisakis pegreffii, Anisakis physeteris, PCRRFLP misc Food processing and manufacture
topic_unstemmed	misc TP368-456 misc parasitic zoonosis, commercial fish, Anisakis pegreffii, Anisakis physeteris, PCRRFLP misc Food processing and manufacture
topic_browse	misc TP368-456 misc parasitic zoonosis, commercial fish, Anisakis pegreffii, Anisakis physeteris, PCRRFLP misc Food processing and manufacture
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hierarchy_parent_title	Italian Journal of Food Safety
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hierarchy_top_title	Italian Journal of Food Safety
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title	FIRST RESULTS ON THE PRESENCE AND THE MOLECULAR CHARACTERIZATION OF ANISAKID NEMATODES IN MARINE FISH CAUGHT OFF NORTHERN SARDINIA
ctrlnum	(DE-627)DOAJ008935378 (DE-599)DOAJc00b36f23d464f7985b52011f7ae57b6
title_full	FIRST RESULTS ON THE PRESENCE AND THE MOLECULAR CHARACTERIZATION OF ANISAKID NEMATODES IN MARINE FISH CAUGHT OFF NORTHERN SARDINIA
author_sort	T. Tedde
journal	Italian Journal of Food Safety
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author_browse	T. Tedde M.C. Piras C. Pinna S. Virgilio G. Terrosu A. Piras G. Garippa P. Merella
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class	TP368-456
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author-letter	T. Tedde
doi_str_mv	10.4081/ijfs.2011.1S.185
author2-role	verfasserin
title_sort	first results on the presence and the molecular characterization of anisakid nematodes in marine fish caught off northern sardinia
callnumber	TP368-456
title_auth	FIRST RESULTS ON THE PRESENCE AND THE MOLECULAR CHARACTERIZATION OF ANISAKID NEMATODES IN MARINE FISH CAUGHT OFF NORTHERN SARDINIA
abstract	Anisakidosis is a parasitic zoonosis caused by nematodes of the family Anisakidae, belonging to the genera Anisakis, Contracaecum and Pseudoterranova. Molecular studies have shown that Anisakis larvae comprise a number of sibling species, which have different genetic structures, hosts and geographical distribution. A great variety of fish species can harbour infectious third stage larvae of this nematode. The preliminary results of a study carried out to evaluate the occurrence of this parasite in commercial fish caught off northern Sardinia are herein reported. From October 2008 to November 2009, 599 specimens of 8 commercial fish species were examined for anisakid larvae through visual inspection of body cavity and peptic digestion of the muscle. Isolated Anisakis sp. larvae were observed at light microscope and identified as Type I or Type II (sensu Berland, 1961). Out of 599 fish examined, 239 (40%) were infected by 1187 anisakid larvae, belonging to the genera Anisakis (1169 Type I and 18 Type II) and Hysterothylacium (692). The molecular identification of Anisakis spp. was carried out on a subsample of 30% of Type I larvae and all Type II larvae. Specimens were firstly examined using a species-specific PCR, with primers designed for Anisakis pegreffii (APEF) and Anisakis physeteris (APHF), and ITS-2 of nuclear rDNA. The results were confirmed by the analysis of the ITS region of nuclear rDNA (ITS-1, 5.8S and ITS-2) using the restriction enzymes HinfI and HhaI in PCR-RFLP. Type I larvae examined were all identified as A. pegreffii, and Type II were all A. physeteris. This is the first contribution to the epidemiology and molecular characterization of Anisakis spp. in commercial fish caught off Sardinia.
abstractGer	Anisakidosis is a parasitic zoonosis caused by nematodes of the family Anisakidae, belonging to the genera Anisakis, Contracaecum and Pseudoterranova. Molecular studies have shown that Anisakis larvae comprise a number of sibling species, which have different genetic structures, hosts and geographical distribution. A great variety of fish species can harbour infectious third stage larvae of this nematode. The preliminary results of a study carried out to evaluate the occurrence of this parasite in commercial fish caught off northern Sardinia are herein reported. From October 2008 to November 2009, 599 specimens of 8 commercial fish species were examined for anisakid larvae through visual inspection of body cavity and peptic digestion of the muscle. Isolated Anisakis sp. larvae were observed at light microscope and identified as Type I or Type II (sensu Berland, 1961). Out of 599 fish examined, 239 (40%) were infected by 1187 anisakid larvae, belonging to the genera Anisakis (1169 Type I and 18 Type II) and Hysterothylacium (692). The molecular identification of Anisakis spp. was carried out on a subsample of 30% of Type I larvae and all Type II larvae. Specimens were firstly examined using a species-specific PCR, with primers designed for Anisakis pegreffii (APEF) and Anisakis physeteris (APHF), and ITS-2 of nuclear rDNA. The results were confirmed by the analysis of the ITS region of nuclear rDNA (ITS-1, 5.8S and ITS-2) using the restriction enzymes HinfI and HhaI in PCR-RFLP. Type I larvae examined were all identified as A. pegreffii, and Type II were all A. physeteris. This is the first contribution to the epidemiology and molecular characterization of Anisakis spp. in commercial fish caught off Sardinia.
abstract_unstemmed	Anisakidosis is a parasitic zoonosis caused by nematodes of the family Anisakidae, belonging to the genera Anisakis, Contracaecum and Pseudoterranova. Molecular studies have shown that Anisakis larvae comprise a number of sibling species, which have different genetic structures, hosts and geographical distribution. A great variety of fish species can harbour infectious third stage larvae of this nematode. The preliminary results of a study carried out to evaluate the occurrence of this parasite in commercial fish caught off northern Sardinia are herein reported. From October 2008 to November 2009, 599 specimens of 8 commercial fish species were examined for anisakid larvae through visual inspection of body cavity and peptic digestion of the muscle. Isolated Anisakis sp. larvae were observed at light microscope and identified as Type I or Type II (sensu Berland, 1961). Out of 599 fish examined, 239 (40%) were infected by 1187 anisakid larvae, belonging to the genera Anisakis (1169 Type I and 18 Type II) and Hysterothylacium (692). The molecular identification of Anisakis spp. was carried out on a subsample of 30% of Type I larvae and all Type II larvae. Specimens were firstly examined using a species-specific PCR, with primers designed for Anisakis pegreffii (APEF) and Anisakis physeteris (APHF), and ITS-2 of nuclear rDNA. The results were confirmed by the analysis of the ITS region of nuclear rDNA (ITS-1, 5.8S and ITS-2) using the restriction enzymes HinfI and HhaI in PCR-RFLP. Type I larvae examined were all identified as A. pegreffii, and Type II were all A. physeteris. This is the first contribution to the epidemiology and molecular characterization of Anisakis spp. in commercial fish caught off Sardinia.
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title_short	FIRST RESULTS ON THE PRESENCE AND THE MOLECULAR CHARACTERIZATION OF ANISAKID NEMATODES IN MARINE FISH CAUGHT OFF NORTHERN SARDINIA
url	https://doi.org/10.4081/ijfs.2011.1S.185 https://doaj.org/article/c00b36f23d464f7985b52011f7ae57b6 http://www.pagepressjournals.org/index.php/ijfs/article/view/861 https://doaj.org/toc/2239-7132
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author2	M.C. Piras C. Pinna S. Virgilio G. Terrosu A. Piras G. Garippa P. Merella
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up_date	2024-07-03T21:01:17.057Z
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