The Effect of Small Cosolutes that Mimic Molecular Crowding Conditions on the Stability of Triplexes Involving Duplex DNA

Triplex stability is studied in crowding conditions using small cosolutes (ethanol, acetonitrile and dimethylsulfoxide) by ultraviolet (UV), circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopies. The results indicate that the triplex is formed preferentially when the triplex fo...
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Autor*in:	Anna Aviñó [verfasserIn] Stefania Mazzini [verfasserIn] Raimundo Gargallo [verfasserIn] Ramon Eritja [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	molecular crowding triplex forming oligonucleotide (TFO) DNA RNA cosolute thermal stability

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 17(2016), 2, p 211
Übergeordnetes Werk:	volume:17 ; year:2016 ; number:2, p 211

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/ijms17020211

Katalog-ID:	DOAJ009178058

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allfieldsSound	10.3390/ijms17020211 doi (DE-627)DOAJ009178058 (DE-599)DOAJ0eecc3a8335340eaa58ba82e995f0b0b DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Anna Aviñó verfasserin aut The Effect of Small Cosolutes that Mimic Molecular Crowding Conditions on the Stability of Triplexes Involving Duplex DNA 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Triplex stability is studied in crowding conditions using small cosolutes (ethanol, acetonitrile and dimethylsulfoxide) by ultraviolet (UV), circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopies. The results indicate that the triplex is formed preferentially when the triplex forming oligonucleotide (TFO) is RNA. In addition, DNA triplexes (D:D·D) are clearly less stable in cosolute solutions while the stability of the RNA triplexes (R:D·D) is only slightly decreased. The kinetic of triplex formation with RNA-TFO is slower than with DNA-TFO and the thermal stability of the triplex is increased with the salt concentration in EtOH-water solutions. Accordingly, RNA could be considered a potential molecule to form a stable triplex for regulatory purposes in molecular crowding conditions. molecular crowding triplex forming oligonucleotide (TFO) DNA RNA cosolute thermal stability Biology (General) Chemistry Stefania Mazzini verfasserin aut Raimundo Gargallo verfasserin aut Ramon Eritja verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 17(2016), 2, p 211 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:17 year:2016 number:2, p 211 https://doi.org/10.3390/ijms17020211 kostenfrei https://doaj.org/article/0eecc3a8335340eaa58ba82e995f0b0b kostenfrei http://www.mdpi.com/1422-0067/17/2/211 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2016 2, p 211
language	English
source	In International Journal of Molecular Sciences 17(2016), 2, p 211 volume:17 year:2016 number:2, p 211
sourceStr	In International Journal of Molecular Sciences 17(2016), 2, p 211 volume:17 year:2016 number:2, p 211
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authorswithroles_txt_mv	Anna Aviñó @@aut@@ Stefania Mazzini @@aut@@ Raimundo Gargallo @@aut@@ Ramon Eritja @@aut@@
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callnumber-first	Q - Science
author	Anna Aviñó
spellingShingle	Anna Aviñó misc QH301-705.5 misc QD1-999 misc molecular crowding misc triplex forming oligonucleotide (TFO) misc DNA misc RNA misc cosolute misc thermal stability misc Biology (General) misc Chemistry The Effect of Small Cosolutes that Mimic Molecular Crowding Conditions on the Stability of Triplexes Involving Duplex DNA
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topic_title	QH301-705.5 QD1-999 The Effect of Small Cosolutes that Mimic Molecular Crowding Conditions on the Stability of Triplexes Involving Duplex DNA molecular crowding triplex forming oligonucleotide (TFO) DNA RNA cosolute thermal stability
topic	misc QH301-705.5 misc QD1-999 misc molecular crowding misc triplex forming oligonucleotide (TFO) misc DNA misc RNA misc cosolute misc thermal stability misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc molecular crowding misc triplex forming oligonucleotide (TFO) misc DNA misc RNA misc cosolute misc thermal stability misc Biology (General) misc Chemistry
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title	The Effect of Small Cosolutes that Mimic Molecular Crowding Conditions on the Stability of Triplexes Involving Duplex DNA
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title_full	The Effect of Small Cosolutes that Mimic Molecular Crowding Conditions on the Stability of Triplexes Involving Duplex DNA
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title_sort	effect of small cosolutes that mimic molecular crowding conditions on the stability of triplexes involving duplex dna
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title_auth	The Effect of Small Cosolutes that Mimic Molecular Crowding Conditions on the Stability of Triplexes Involving Duplex DNA
abstract	Triplex stability is studied in crowding conditions using small cosolutes (ethanol, acetonitrile and dimethylsulfoxide) by ultraviolet (UV), circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopies. The results indicate that the triplex is formed preferentially when the triplex forming oligonucleotide (TFO) is RNA. In addition, DNA triplexes (D:D·D) are clearly less stable in cosolute solutions while the stability of the RNA triplexes (R:D·D) is only slightly decreased. The kinetic of triplex formation with RNA-TFO is slower than with DNA-TFO and the thermal stability of the triplex is increased with the salt concentration in EtOH-water solutions. Accordingly, RNA could be considered a potential molecule to form a stable triplex for regulatory purposes in molecular crowding conditions.
abstractGer	Triplex stability is studied in crowding conditions using small cosolutes (ethanol, acetonitrile and dimethylsulfoxide) by ultraviolet (UV), circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopies. The results indicate that the triplex is formed preferentially when the triplex forming oligonucleotide (TFO) is RNA. In addition, DNA triplexes (D:D·D) are clearly less stable in cosolute solutions while the stability of the RNA triplexes (R:D·D) is only slightly decreased. The kinetic of triplex formation with RNA-TFO is slower than with DNA-TFO and the thermal stability of the triplex is increased with the salt concentration in EtOH-water solutions. Accordingly, RNA could be considered a potential molecule to form a stable triplex for regulatory purposes in molecular crowding conditions.
abstract_unstemmed	Triplex stability is studied in crowding conditions using small cosolutes (ethanol, acetonitrile and dimethylsulfoxide) by ultraviolet (UV), circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopies. The results indicate that the triplex is formed preferentially when the triplex forming oligonucleotide (TFO) is RNA. In addition, DNA triplexes (D:D·D) are clearly less stable in cosolute solutions while the stability of the RNA triplexes (R:D·D) is only slightly decreased. The kinetic of triplex formation with RNA-TFO is slower than with DNA-TFO and the thermal stability of the triplex is increased with the salt concentration in EtOH-water solutions. Accordingly, RNA could be considered a potential molecule to form a stable triplex for regulatory purposes in molecular crowding conditions.
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title_short	The Effect of Small Cosolutes that Mimic Molecular Crowding Conditions on the Stability of Triplexes Involving Duplex DNA
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