LINK-A promotes cell proliferation through the regulation of aerobic glycolysis in non-small-cell lung cancer

Wei Zhao,1,2 Wancheng Li,3 Wenjing Dai,3 Na Huang,3 Jing Qiu3 1Department of Clinical Biochemistry, School of Laboratory Medicine, Chengdu Medical College, Chengdu, People’s Republic of China; 2Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, People’s Republic of China; 3Department of Respiratory Medicine, The First Affiliated Hospital of Chengdu Medical College, Chengdu, People’s Republic of China Purpose: Non-small-cell lung cancer (NSCLC) is the one of the most common malignancies worldwide, and occurs at a higher frequency in male individuals. Little is known about the role of the long intergenic noncoding RNA for kinase activation (LINK-A) in NSCLC, so in the present study we assessed its potential role on cell proliferation in NSCLC. Methods: Expression levels of LINK-A in NSCLC tissues and cell lines were detected by quantitative reverse-transcription polymerase chain reaction. LINK-A was knocked down and overexpressed separately in A549 cells and NCI-H1299 cells. The effect of LINK-A expression on cell proliferation was determined by MTT assay. The correlation between LINK-A and hexokinase II (HKII) expression was investigated by Western blot and HKII Activity Assay. Glucose consumption and lactate production assay were used to investigate the aerobic glycolysis in NSCLC cells. The effect of LINK-A in vivo was determined by xenograft assay. Results: LINK-A expression levels were increased in NSCLC tissues compared with normal tissues. Moreover, LINK-A expression was positively correlated with NSCLC clinicopathological characteristics and survival rate, while knockdown of LINK-A reduced NSCLC cell proliferation. LINK-A expression was also positively correlated with HKII, and NSCLC cells with low LINK-A expression were found to have significantly reduced HKII protein expression, accompanied by a reduction in enzyme activity levels. Both in vitro and in vivo experiments showed that LINK-A expression affected glucose consumption and lactate production through regulation of HKII expression. Conclusion: These data suggest that the functions of LINK-A in NSCLC might play a key role in tumor progression and that LINK-A could be a promising predictive biomarker and potential therapeutic target for NSCLC. Keywords: non-small-cell lung cancer, LINK-A, proliferation, hexokinase II, aerobic glycolysis, prognosis Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Zhao W [verfasserIn] Li W [verfasserIn] Dai W [verfasserIn] Huang N [verfasserIn] Qiu J [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	Non-small cell lung cancer ∙ LINK-A ∙ Proliferation ∙ Hexokinase II ∙ Aerobic glycolysis ∙ Prognosis

Übergeordnetes Werk:	In: OncoTargets and Therapy - Dove Medical Press, 2009, (2018), Seite 6071-6080
Übergeordnetes Werk:	year:2018 ; pages:6071-6080

Links:	Link aufrufen Link aufrufen Journal toc

Katalog-ID:	DOAJ009203923

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allfields	(DE-627)DOAJ009203923 (DE-599)DOAJ7313b67e23ce40b9bc30b88bb8455f84 DE-627 ger DE-627 rakwb eng RC254-282 Zhao W verfasserin aut LINK-A promotes cell proliferation through the regulation of aerobic glycolysis in non-small-cell lung cancer 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Wei Zhao,1,2 Wancheng Li,3 Wenjing Dai,3 Na Huang,3 Jing Qiu3 1Department of Clinical Biochemistry, School of Laboratory Medicine, Chengdu Medical College, Chengdu, People’s Republic of China; 2Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, People’s Republic of China; 3Department of Respiratory Medicine, The First Affiliated Hospital of Chengdu Medical College, Chengdu, People’s Republic of China Purpose: Non-small-cell lung cancer (NSCLC) is the one of the most common malignancies worldwide, and occurs at a higher frequency in male individuals. Little is known about the role of the long intergenic noncoding RNA for kinase activation (LINK-A) in NSCLC, so in the present study we assessed its potential role on cell proliferation in NSCLC. Methods: Expression levels of LINK-A in NSCLC tissues and cell lines were detected by quantitative reverse-transcription polymerase chain reaction. LINK-A was knocked down and overexpressed separately in A549 cells and NCI-H1299 cells. The effect of LINK-A expression on cell proliferation was determined by MTT assay. The correlation between LINK-A and hexokinase II (HKII) expression was investigated by Western blot and HKII Activity Assay. Glucose consumption and lactate production assay were used to investigate the aerobic glycolysis in NSCLC cells. The effect of LINK-A in vivo was determined by xenograft assay. Results: LINK-A expression levels were increased in NSCLC tissues compared with normal tissues. Moreover, LINK-A expression was positively correlated with NSCLC clinicopathological characteristics and survival rate, while knockdown of LINK-A reduced NSCLC cell proliferation. LINK-A expression was also positively correlated with HKII, and NSCLC cells with low LINK-A expression were found to have significantly reduced HKII protein expression, accompanied by a reduction in enzyme activity levels. Both in vitro and in vivo experiments showed that LINK-A expression affected glucose consumption and lactate production through regulation of HKII expression. Conclusion: These data suggest that the functions of LINK-A in NSCLC might play a key role in tumor progression and that LINK-A could be a promising predictive biomarker and potential therapeutic target for NSCLC. Keywords: non-small-cell lung cancer, LINK-A, proliferation, hexokinase II, aerobic glycolysis, prognosis Non-small cell lung cancer ∙ LINK-A ∙ Proliferation ∙ Hexokinase II ∙ Aerobic glycolysis ∙ Prognosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens Li W verfasserin aut Dai W verfasserin aut Huang N verfasserin aut Qiu J verfasserin aut In OncoTargets and Therapy Dove Medical Press, 2009 (2018), Seite 6071-6080 (DE-627)600307654 (DE-600)2495130-4 11786930 nnns year:2018 pages:6071-6080 https://doaj.org/article/7313b67e23ce40b9bc30b88bb8455f84 kostenfrei https://www.dovepress.com/link-a-promotes-cell-proliferation-through-the-regulation-of-aerobic-g-peer-reviewed-article-OTT kostenfrei https://doaj.org/toc/1178-6930 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2018 6071-6080
spelling	(DE-627)DOAJ009203923 (DE-599)DOAJ7313b67e23ce40b9bc30b88bb8455f84 DE-627 ger DE-627 rakwb eng RC254-282 Zhao W verfasserin aut LINK-A promotes cell proliferation through the regulation of aerobic glycolysis in non-small-cell lung cancer 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Wei Zhao,1,2 Wancheng Li,3 Wenjing Dai,3 Na Huang,3 Jing Qiu3 1Department of Clinical Biochemistry, School of Laboratory Medicine, Chengdu Medical College, Chengdu, People’s Republic of China; 2Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, People’s Republic of China; 3Department of Respiratory Medicine, The First Affiliated Hospital of Chengdu Medical College, Chengdu, People’s Republic of China Purpose: Non-small-cell lung cancer (NSCLC) is the one of the most common malignancies worldwide, and occurs at a higher frequency in male individuals. Little is known about the role of the long intergenic noncoding RNA for kinase activation (LINK-A) in NSCLC, so in the present study we assessed its potential role on cell proliferation in NSCLC. Methods: Expression levels of LINK-A in NSCLC tissues and cell lines were detected by quantitative reverse-transcription polymerase chain reaction. LINK-A was knocked down and overexpressed separately in A549 cells and NCI-H1299 cells. The effect of LINK-A expression on cell proliferation was determined by MTT assay. The correlation between LINK-A and hexokinase II (HKII) expression was investigated by Western blot and HKII Activity Assay. Glucose consumption and lactate production assay were used to investigate the aerobic glycolysis in NSCLC cells. The effect of LINK-A in vivo was determined by xenograft assay. Results: LINK-A expression levels were increased in NSCLC tissues compared with normal tissues. Moreover, LINK-A expression was positively correlated with NSCLC clinicopathological characteristics and survival rate, while knockdown of LINK-A reduced NSCLC cell proliferation. LINK-A expression was also positively correlated with HKII, and NSCLC cells with low LINK-A expression were found to have significantly reduced HKII protein expression, accompanied by a reduction in enzyme activity levels. Both in vitro and in vivo experiments showed that LINK-A expression affected glucose consumption and lactate production through regulation of HKII expression. Conclusion: These data suggest that the functions of LINK-A in NSCLC might play a key role in tumor progression and that LINK-A could be a promising predictive biomarker and potential therapeutic target for NSCLC. Keywords: non-small-cell lung cancer, LINK-A, proliferation, hexokinase II, aerobic glycolysis, prognosis Non-small cell lung cancer ∙ LINK-A ∙ Proliferation ∙ Hexokinase II ∙ Aerobic glycolysis ∙ Prognosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens Li W verfasserin aut Dai W verfasserin aut Huang N verfasserin aut Qiu J verfasserin aut In OncoTargets and Therapy Dove Medical Press, 2009 (2018), Seite 6071-6080 (DE-627)600307654 (DE-600)2495130-4 11786930 nnns year:2018 pages:6071-6080 https://doaj.org/article/7313b67e23ce40b9bc30b88bb8455f84 kostenfrei https://www.dovepress.com/link-a-promotes-cell-proliferation-through-the-regulation-of-aerobic-g-peer-reviewed-article-OTT kostenfrei https://doaj.org/toc/1178-6930 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2018 6071-6080
allfields_unstemmed	(DE-627)DOAJ009203923 (DE-599)DOAJ7313b67e23ce40b9bc30b88bb8455f84 DE-627 ger DE-627 rakwb eng RC254-282 Zhao W verfasserin aut LINK-A promotes cell proliferation through the regulation of aerobic glycolysis in non-small-cell lung cancer 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Wei Zhao,1,2 Wancheng Li,3 Wenjing Dai,3 Na Huang,3 Jing Qiu3 1Department of Clinical Biochemistry, School of Laboratory Medicine, Chengdu Medical College, Chengdu, People’s Republic of China; 2Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, People’s Republic of China; 3Department of Respiratory Medicine, The First Affiliated Hospital of Chengdu Medical College, Chengdu, People’s Republic of China Purpose: Non-small-cell lung cancer (NSCLC) is the one of the most common malignancies worldwide, and occurs at a higher frequency in male individuals. Little is known about the role of the long intergenic noncoding RNA for kinase activation (LINK-A) in NSCLC, so in the present study we assessed its potential role on cell proliferation in NSCLC. Methods: Expression levels of LINK-A in NSCLC tissues and cell lines were detected by quantitative reverse-transcription polymerase chain reaction. LINK-A was knocked down and overexpressed separately in A549 cells and NCI-H1299 cells. The effect of LINK-A expression on cell proliferation was determined by MTT assay. The correlation between LINK-A and hexokinase II (HKII) expression was investigated by Western blot and HKII Activity Assay. Glucose consumption and lactate production assay were used to investigate the aerobic glycolysis in NSCLC cells. The effect of LINK-A in vivo was determined by xenograft assay. Results: LINK-A expression levels were increased in NSCLC tissues compared with normal tissues. Moreover, LINK-A expression was positively correlated with NSCLC clinicopathological characteristics and survival rate, while knockdown of LINK-A reduced NSCLC cell proliferation. LINK-A expression was also positively correlated with HKII, and NSCLC cells with low LINK-A expression were found to have significantly reduced HKII protein expression, accompanied by a reduction in enzyme activity levels. Both in vitro and in vivo experiments showed that LINK-A expression affected glucose consumption and lactate production through regulation of HKII expression. Conclusion: These data suggest that the functions of LINK-A in NSCLC might play a key role in tumor progression and that LINK-A could be a promising predictive biomarker and potential therapeutic target for NSCLC. Keywords: non-small-cell lung cancer, LINK-A, proliferation, hexokinase II, aerobic glycolysis, prognosis Non-small cell lung cancer ∙ LINK-A ∙ Proliferation ∙ Hexokinase II ∙ Aerobic glycolysis ∙ Prognosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens Li W verfasserin aut Dai W verfasserin aut Huang N verfasserin aut Qiu J verfasserin aut In OncoTargets and Therapy Dove Medical Press, 2009 (2018), Seite 6071-6080 (DE-627)600307654 (DE-600)2495130-4 11786930 nnns year:2018 pages:6071-6080 https://doaj.org/article/7313b67e23ce40b9bc30b88bb8455f84 kostenfrei https://www.dovepress.com/link-a-promotes-cell-proliferation-through-the-regulation-of-aerobic-g-peer-reviewed-article-OTT kostenfrei https://doaj.org/toc/1178-6930 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2018 6071-6080
allfieldsGer	(DE-627)DOAJ009203923 (DE-599)DOAJ7313b67e23ce40b9bc30b88bb8455f84 DE-627 ger DE-627 rakwb eng RC254-282 Zhao W verfasserin aut LINK-A promotes cell proliferation through the regulation of aerobic glycolysis in non-small-cell lung cancer 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Wei Zhao,1,2 Wancheng Li,3 Wenjing Dai,3 Na Huang,3 Jing Qiu3 1Department of Clinical Biochemistry, School of Laboratory Medicine, Chengdu Medical College, Chengdu, People’s Republic of China; 2Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, People’s Republic of China; 3Department of Respiratory Medicine, The First Affiliated Hospital of Chengdu Medical College, Chengdu, People’s Republic of China Purpose: Non-small-cell lung cancer (NSCLC) is the one of the most common malignancies worldwide, and occurs at a higher frequency in male individuals. Little is known about the role of the long intergenic noncoding RNA for kinase activation (LINK-A) in NSCLC, so in the present study we assessed its potential role on cell proliferation in NSCLC. Methods: Expression levels of LINK-A in NSCLC tissues and cell lines were detected by quantitative reverse-transcription polymerase chain reaction. LINK-A was knocked down and overexpressed separately in A549 cells and NCI-H1299 cells. The effect of LINK-A expression on cell proliferation was determined by MTT assay. The correlation between LINK-A and hexokinase II (HKII) expression was investigated by Western blot and HKII Activity Assay. Glucose consumption and lactate production assay were used to investigate the aerobic glycolysis in NSCLC cells. The effect of LINK-A in vivo was determined by xenograft assay. Results: LINK-A expression levels were increased in NSCLC tissues compared with normal tissues. Moreover, LINK-A expression was positively correlated with NSCLC clinicopathological characteristics and survival rate, while knockdown of LINK-A reduced NSCLC cell proliferation. LINK-A expression was also positively correlated with HKII, and NSCLC cells with low LINK-A expression were found to have significantly reduced HKII protein expression, accompanied by a reduction in enzyme activity levels. Both in vitro and in vivo experiments showed that LINK-A expression affected glucose consumption and lactate production through regulation of HKII expression. Conclusion: These data suggest that the functions of LINK-A in NSCLC might play a key role in tumor progression and that LINK-A could be a promising predictive biomarker and potential therapeutic target for NSCLC. Keywords: non-small-cell lung cancer, LINK-A, proliferation, hexokinase II, aerobic glycolysis, prognosis Non-small cell lung cancer ∙ LINK-A ∙ Proliferation ∙ Hexokinase II ∙ Aerobic glycolysis ∙ Prognosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens Li W verfasserin aut Dai W verfasserin aut Huang N verfasserin aut Qiu J verfasserin aut In OncoTargets and Therapy Dove Medical Press, 2009 (2018), Seite 6071-6080 (DE-627)600307654 (DE-600)2495130-4 11786930 nnns year:2018 pages:6071-6080 https://doaj.org/article/7313b67e23ce40b9bc30b88bb8455f84 kostenfrei https://www.dovepress.com/link-a-promotes-cell-proliferation-through-the-regulation-of-aerobic-g-peer-reviewed-article-OTT kostenfrei https://doaj.org/toc/1178-6930 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2018 6071-6080
allfieldsSound	(DE-627)DOAJ009203923 (DE-599)DOAJ7313b67e23ce40b9bc30b88bb8455f84 DE-627 ger DE-627 rakwb eng RC254-282 Zhao W verfasserin aut LINK-A promotes cell proliferation through the regulation of aerobic glycolysis in non-small-cell lung cancer 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Wei Zhao,1,2 Wancheng Li,3 Wenjing Dai,3 Na Huang,3 Jing Qiu3 1Department of Clinical Biochemistry, School of Laboratory Medicine, Chengdu Medical College, Chengdu, People’s Republic of China; 2Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, People’s Republic of China; 3Department of Respiratory Medicine, The First Affiliated Hospital of Chengdu Medical College, Chengdu, People’s Republic of China Purpose: Non-small-cell lung cancer (NSCLC) is the one of the most common malignancies worldwide, and occurs at a higher frequency in male individuals. Little is known about the role of the long intergenic noncoding RNA for kinase activation (LINK-A) in NSCLC, so in the present study we assessed its potential role on cell proliferation in NSCLC. Methods: Expression levels of LINK-A in NSCLC tissues and cell lines were detected by quantitative reverse-transcription polymerase chain reaction. LINK-A was knocked down and overexpressed separately in A549 cells and NCI-H1299 cells. The effect of LINK-A expression on cell proliferation was determined by MTT assay. The correlation between LINK-A and hexokinase II (HKII) expression was investigated by Western blot and HKII Activity Assay. Glucose consumption and lactate production assay were used to investigate the aerobic glycolysis in NSCLC cells. The effect of LINK-A in vivo was determined by xenograft assay. Results: LINK-A expression levels were increased in NSCLC tissues compared with normal tissues. Moreover, LINK-A expression was positively correlated with NSCLC clinicopathological characteristics and survival rate, while knockdown of LINK-A reduced NSCLC cell proliferation. LINK-A expression was also positively correlated with HKII, and NSCLC cells with low LINK-A expression were found to have significantly reduced HKII protein expression, accompanied by a reduction in enzyme activity levels. Both in vitro and in vivo experiments showed that LINK-A expression affected glucose consumption and lactate production through regulation of HKII expression. Conclusion: These data suggest that the functions of LINK-A in NSCLC might play a key role in tumor progression and that LINK-A could be a promising predictive biomarker and potential therapeutic target for NSCLC. Keywords: non-small-cell lung cancer, LINK-A, proliferation, hexokinase II, aerobic glycolysis, prognosis Non-small cell lung cancer ∙ LINK-A ∙ Proliferation ∙ Hexokinase II ∙ Aerobic glycolysis ∙ Prognosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens Li W verfasserin aut Dai W verfasserin aut Huang N verfasserin aut Qiu J verfasserin aut In OncoTargets and Therapy Dove Medical Press, 2009 (2018), Seite 6071-6080 (DE-627)600307654 (DE-600)2495130-4 11786930 nnns year:2018 pages:6071-6080 https://doaj.org/article/7313b67e23ce40b9bc30b88bb8455f84 kostenfrei https://www.dovepress.com/link-a-promotes-cell-proliferation-through-the-regulation-of-aerobic-g-peer-reviewed-article-OTT kostenfrei https://doaj.org/toc/1178-6930 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2018 6071-6080
language	English
source	In OncoTargets and Therapy (2018), Seite 6071-6080 year:2018 pages:6071-6080
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topic_facet	Non-small cell lung cancer ∙ LINK-A ∙ Proliferation ∙ Hexokinase II ∙ Aerobic glycolysis ∙ Prognosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens
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Little is known about the role of the long intergenic noncoding RNA for kinase activation (LINK-A) in NSCLC, so in the present study we assessed its potential role on cell proliferation in NSCLC. Methods: Expression levels of LINK-A in NSCLC tissues and cell lines were detected by quantitative reverse-transcription polymerase chain reaction. LINK-A was knocked down and overexpressed separately in A549 cells and NCI-H1299 cells. The effect of LINK-A expression on cell proliferation was determined by MTT assay. The correlation between LINK-A and hexokinase II (HKII) expression was investigated by Western blot and HKII Activity Assay. Glucose consumption and lactate production assay were used to investigate the aerobic glycolysis in NSCLC cells. The effect of LINK-A in vivo was determined by xenograft assay. Results: LINK-A expression levels were increased in NSCLC tissues compared with normal tissues. Moreover, LINK-A expression was positively correlated with NSCLC clinicopathological characteristics and survival rate, while knockdown of LINK-A reduced NSCLC cell proliferation. LINK-A expression was also positively correlated with HKII, and NSCLC cells with low LINK-A expression were found to have significantly reduced HKII protein expression, accompanied by a reduction in enzyme activity levels. Both in vitro and in vivo experiments showed that LINK-A expression affected glucose consumption and lactate production through regulation of HKII expression. Conclusion: These data suggest that the functions of LINK-A in NSCLC might play a key role in tumor progression and that LINK-A could be a promising predictive biomarker and potential therapeutic target for NSCLC. 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topic_title	RC254-282 LINK-A promotes cell proliferation through the regulation of aerobic glycolysis in non-small-cell lung cancer Non-small cell lung cancer ∙ LINK-A ∙ Proliferation ∙ Hexokinase II ∙ Aerobic glycolysis ∙ Prognosis
topic	misc RC254-282 misc Non-small cell lung cancer ∙ LINK-A ∙ Proliferation ∙ Hexokinase II ∙ Aerobic glycolysis ∙ Prognosis misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
topic_unstemmed	misc RC254-282 misc Non-small cell lung cancer ∙ LINK-A ∙ Proliferation ∙ Hexokinase II ∙ Aerobic glycolysis ∙ Prognosis misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
topic_browse	misc RC254-282 misc Non-small cell lung cancer ∙ LINK-A ∙ Proliferation ∙ Hexokinase II ∙ Aerobic glycolysis ∙ Prognosis misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
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title	LINK-A promotes cell proliferation through the regulation of aerobic glycolysis in non-small-cell lung cancer
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title_full	LINK-A promotes cell proliferation through the regulation of aerobic glycolysis in non-small-cell lung cancer
author_sort	Zhao W
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author_browse	Zhao W Li W Dai W Huang N Qiu J
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title_sort	link-a promotes cell proliferation through the regulation of aerobic glycolysis in non-small-cell lung cancer
callnumber	RC254-282
title_auth	LINK-A promotes cell proliferation through the regulation of aerobic glycolysis in non-small-cell lung cancer
abstract	Wei Zhao,1,2 Wancheng Li,3 Wenjing Dai,3 Na Huang,3 Jing Qiu3 1Department of Clinical Biochemistry, School of Laboratory Medicine, Chengdu Medical College, Chengdu, People’s Republic of China; 2Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, People’s Republic of China; 3Department of Respiratory Medicine, The First Affiliated Hospital of Chengdu Medical College, Chengdu, People’s Republic of China Purpose: Non-small-cell lung cancer (NSCLC) is the one of the most common malignancies worldwide, and occurs at a higher frequency in male individuals. Little is known about the role of the long intergenic noncoding RNA for kinase activation (LINK-A) in NSCLC, so in the present study we assessed its potential role on cell proliferation in NSCLC. Methods: Expression levels of LINK-A in NSCLC tissues and cell lines were detected by quantitative reverse-transcription polymerase chain reaction. LINK-A was knocked down and overexpressed separately in A549 cells and NCI-H1299 cells. The effect of LINK-A expression on cell proliferation was determined by MTT assay. The correlation between LINK-A and hexokinase II (HKII) expression was investigated by Western blot and HKII Activity Assay. Glucose consumption and lactate production assay were used to investigate the aerobic glycolysis in NSCLC cells. The effect of LINK-A in vivo was determined by xenograft assay. Results: LINK-A expression levels were increased in NSCLC tissues compared with normal tissues. Moreover, LINK-A expression was positively correlated with NSCLC clinicopathological characteristics and survival rate, while knockdown of LINK-A reduced NSCLC cell proliferation. LINK-A expression was also positively correlated with HKII, and NSCLC cells with low LINK-A expression were found to have significantly reduced HKII protein expression, accompanied by a reduction in enzyme activity levels. Both in vitro and in vivo experiments showed that LINK-A expression affected glucose consumption and lactate production through regulation of HKII expression. Conclusion: These data suggest that the functions of LINK-A in NSCLC might play a key role in tumor progression and that LINK-A could be a promising predictive biomarker and potential therapeutic target for NSCLC. Keywords: non-small-cell lung cancer, LINK-A, proliferation, hexokinase II, aerobic glycolysis, prognosis
abstractGer	Wei Zhao,1,2 Wancheng Li,3 Wenjing Dai,3 Na Huang,3 Jing Qiu3 1Department of Clinical Biochemistry, School of Laboratory Medicine, Chengdu Medical College, Chengdu, People’s Republic of China; 2Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, People’s Republic of China; 3Department of Respiratory Medicine, The First Affiliated Hospital of Chengdu Medical College, Chengdu, People’s Republic of China Purpose: Non-small-cell lung cancer (NSCLC) is the one of the most common malignancies worldwide, and occurs at a higher frequency in male individuals. Little is known about the role of the long intergenic noncoding RNA for kinase activation (LINK-A) in NSCLC, so in the present study we assessed its potential role on cell proliferation in NSCLC. Methods: Expression levels of LINK-A in NSCLC tissues and cell lines were detected by quantitative reverse-transcription polymerase chain reaction. LINK-A was knocked down and overexpressed separately in A549 cells and NCI-H1299 cells. The effect of LINK-A expression on cell proliferation was determined by MTT assay. The correlation between LINK-A and hexokinase II (HKII) expression was investigated by Western blot and HKII Activity Assay. Glucose consumption and lactate production assay were used to investigate the aerobic glycolysis in NSCLC cells. The effect of LINK-A in vivo was determined by xenograft assay. Results: LINK-A expression levels were increased in NSCLC tissues compared with normal tissues. Moreover, LINK-A expression was positively correlated with NSCLC clinicopathological characteristics and survival rate, while knockdown of LINK-A reduced NSCLC cell proliferation. LINK-A expression was also positively correlated with HKII, and NSCLC cells with low LINK-A expression were found to have significantly reduced HKII protein expression, accompanied by a reduction in enzyme activity levels. Both in vitro and in vivo experiments showed that LINK-A expression affected glucose consumption and lactate production through regulation of HKII expression. Conclusion: These data suggest that the functions of LINK-A in NSCLC might play a key role in tumor progression and that LINK-A could be a promising predictive biomarker and potential therapeutic target for NSCLC. Keywords: non-small-cell lung cancer, LINK-A, proliferation, hexokinase II, aerobic glycolysis, prognosis
abstract_unstemmed	Wei Zhao,1,2 Wancheng Li,3 Wenjing Dai,3 Na Huang,3 Jing Qiu3 1Department of Clinical Biochemistry, School of Laboratory Medicine, Chengdu Medical College, Chengdu, People’s Republic of China; 2Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, People’s Republic of China; 3Department of Respiratory Medicine, The First Affiliated Hospital of Chengdu Medical College, Chengdu, People’s Republic of China Purpose: Non-small-cell lung cancer (NSCLC) is the one of the most common malignancies worldwide, and occurs at a higher frequency in male individuals. Little is known about the role of the long intergenic noncoding RNA for kinase activation (LINK-A) in NSCLC, so in the present study we assessed its potential role on cell proliferation in NSCLC. Methods: Expression levels of LINK-A in NSCLC tissues and cell lines were detected by quantitative reverse-transcription polymerase chain reaction. LINK-A was knocked down and overexpressed separately in A549 cells and NCI-H1299 cells. The effect of LINK-A expression on cell proliferation was determined by MTT assay. The correlation between LINK-A and hexokinase II (HKII) expression was investigated by Western blot and HKII Activity Assay. Glucose consumption and lactate production assay were used to investigate the aerobic glycolysis in NSCLC cells. The effect of LINK-A in vivo was determined by xenograft assay. Results: LINK-A expression levels were increased in NSCLC tissues compared with normal tissues. Moreover, LINK-A expression was positively correlated with NSCLC clinicopathological characteristics and survival rate, while knockdown of LINK-A reduced NSCLC cell proliferation. LINK-A expression was also positively correlated with HKII, and NSCLC cells with low LINK-A expression were found to have significantly reduced HKII protein expression, accompanied by a reduction in enzyme activity levels. Both in vitro and in vivo experiments showed that LINK-A expression affected glucose consumption and lactate production through regulation of HKII expression. Conclusion: These data suggest that the functions of LINK-A in NSCLC might play a key role in tumor progression and that LINK-A could be a promising predictive biomarker and potential therapeutic target for NSCLC. Keywords: non-small-cell lung cancer, LINK-A, proliferation, hexokinase II, aerobic glycolysis, prognosis
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title_short	LINK-A promotes cell proliferation through the regulation of aerobic glycolysis in non-small-cell lung cancer
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