ATP-induced IL-1β specific secretion: true under stringent conditions.

Interleukin-1β is a potent proinflammatory cytokine, of which processing and secretion are tightly regulated. After exposure to various stimuli, mononuclear phagocytes synthesize the inactive precursor (pro-IL-1β), which is then cleaved intracellularly by caspase-1 and secreted. A widely used method...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Monique eStoffels [verfasserIn] Ruben eZaal [verfasserIn] Nina eKok [verfasserIn] Jos W.M. van der Meer [verfasserIn] Charles A. Dinarello [verfasserIn] Anna eSimon [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	Cell Death Interleukin-1 ATP active secretion stringent experimental conditions.

Übergeordnetes Werk:	In: Frontiers in Immunology - Frontiers Media S.A., 2011, 6(2015)
Übergeordnetes Werk:	volume:6 ; year:2015

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fimmu.2015.00054

Katalog-ID:	DOAJ009214895

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650		4	\|a Cell Death
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author_variant	m e me m e me r e re r e re n e ne n e ne j w v d m jwvdm j w v d m jwvdm c a d cad c a d cad a e ae a e ae
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allfields	10.3389/fimmu.2015.00054 doi (DE-627)DOAJ009214895 (DE-599)DOAJ26ada269dc02429ba3f6da5b6cbc9631 DE-627 ger DE-627 rakwb eng RC581-607 Monique eStoffels verfasserin aut ATP-induced IL-1β specific secretion: true under stringent conditions. 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Interleukin-1β is a potent proinflammatory cytokine, of which processing and secretion are tightly regulated. After exposure to various stimuli, mononuclear phagocytes synthesize the inactive precursor (pro-IL-1β), which is then cleaved intracellularly by caspase-1 and secreted. A widely used method for in-vitro secretion of IL-1β employs LPS-primed human peripheral blood monocytes. Subsequently, adenosine triphosphate (ATP) is added to the cells in order to trigger the P2X7 receptor resulting in processing and secretion of mature IL-1β. However, it is often reported that secretion is due to cytotoxic effects of ATP with P2X7 receptor-activation-related cell death. We have challenged this concept and demonstrate IL-1β specific secretion, since there is no increase in cell death and IL-1α and IL-18 are not released in the same cultures. More importantly we show that these conclusions can only be drawn under stringent experimental conditions. Cell Death Interleukin-1 ATP active secretion stringent experimental conditions. Immunologic diseases. Allergy Monique eStoffels verfasserin aut Ruben eZaal verfasserin aut Ruben eZaal verfasserin aut Nina eKok verfasserin aut Nina eKok verfasserin aut Jos W.M. van der Meer verfasserin aut Jos W.M. van der Meer verfasserin aut Charles A. Dinarello verfasserin aut Charles A. Dinarello verfasserin aut Anna eSimon verfasserin aut Anna eSimon verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 6(2015) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:6 year:2015 https://doi.org/10.3389/fimmu.2015.00054 kostenfrei https://doaj.org/article/26ada269dc02429ba3f6da5b6cbc9631 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00054/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
spelling	10.3389/fimmu.2015.00054 doi (DE-627)DOAJ009214895 (DE-599)DOAJ26ada269dc02429ba3f6da5b6cbc9631 DE-627 ger DE-627 rakwb eng RC581-607 Monique eStoffels verfasserin aut ATP-induced IL-1β specific secretion: true under stringent conditions. 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Interleukin-1β is a potent proinflammatory cytokine, of which processing and secretion are tightly regulated. After exposure to various stimuli, mononuclear phagocytes synthesize the inactive precursor (pro-IL-1β), which is then cleaved intracellularly by caspase-1 and secreted. A widely used method for in-vitro secretion of IL-1β employs LPS-primed human peripheral blood monocytes. Subsequently, adenosine triphosphate (ATP) is added to the cells in order to trigger the P2X7 receptor resulting in processing and secretion of mature IL-1β. However, it is often reported that secretion is due to cytotoxic effects of ATP with P2X7 receptor-activation-related cell death. We have challenged this concept and demonstrate IL-1β specific secretion, since there is no increase in cell death and IL-1α and IL-18 are not released in the same cultures. More importantly we show that these conclusions can only be drawn under stringent experimental conditions. Cell Death Interleukin-1 ATP active secretion stringent experimental conditions. Immunologic diseases. Allergy Monique eStoffels verfasserin aut Ruben eZaal verfasserin aut Ruben eZaal verfasserin aut Nina eKok verfasserin aut Nina eKok verfasserin aut Jos W.M. van der Meer verfasserin aut Jos W.M. van der Meer verfasserin aut Charles A. Dinarello verfasserin aut Charles A. Dinarello verfasserin aut Anna eSimon verfasserin aut Anna eSimon verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 6(2015) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:6 year:2015 https://doi.org/10.3389/fimmu.2015.00054 kostenfrei https://doaj.org/article/26ada269dc02429ba3f6da5b6cbc9631 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00054/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
allfields_unstemmed	10.3389/fimmu.2015.00054 doi (DE-627)DOAJ009214895 (DE-599)DOAJ26ada269dc02429ba3f6da5b6cbc9631 DE-627 ger DE-627 rakwb eng RC581-607 Monique eStoffels verfasserin aut ATP-induced IL-1β specific secretion: true under stringent conditions. 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Interleukin-1β is a potent proinflammatory cytokine, of which processing and secretion are tightly regulated. After exposure to various stimuli, mononuclear phagocytes synthesize the inactive precursor (pro-IL-1β), which is then cleaved intracellularly by caspase-1 and secreted. A widely used method for in-vitro secretion of IL-1β employs LPS-primed human peripheral blood monocytes. Subsequently, adenosine triphosphate (ATP) is added to the cells in order to trigger the P2X7 receptor resulting in processing and secretion of mature IL-1β. However, it is often reported that secretion is due to cytotoxic effects of ATP with P2X7 receptor-activation-related cell death. We have challenged this concept and demonstrate IL-1β specific secretion, since there is no increase in cell death and IL-1α and IL-18 are not released in the same cultures. More importantly we show that these conclusions can only be drawn under stringent experimental conditions. Cell Death Interleukin-1 ATP active secretion stringent experimental conditions. Immunologic diseases. Allergy Monique eStoffels verfasserin aut Ruben eZaal verfasserin aut Ruben eZaal verfasserin aut Nina eKok verfasserin aut Nina eKok verfasserin aut Jos W.M. van der Meer verfasserin aut Jos W.M. van der Meer verfasserin aut Charles A. Dinarello verfasserin aut Charles A. Dinarello verfasserin aut Anna eSimon verfasserin aut Anna eSimon verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 6(2015) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:6 year:2015 https://doi.org/10.3389/fimmu.2015.00054 kostenfrei https://doaj.org/article/26ada269dc02429ba3f6da5b6cbc9631 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00054/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
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allfieldsSound	10.3389/fimmu.2015.00054 doi (DE-627)DOAJ009214895 (DE-599)DOAJ26ada269dc02429ba3f6da5b6cbc9631 DE-627 ger DE-627 rakwb eng RC581-607 Monique eStoffels verfasserin aut ATP-induced IL-1β specific secretion: true under stringent conditions. 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Interleukin-1β is a potent proinflammatory cytokine, of which processing and secretion are tightly regulated. After exposure to various stimuli, mononuclear phagocytes synthesize the inactive precursor (pro-IL-1β), which is then cleaved intracellularly by caspase-1 and secreted. A widely used method for in-vitro secretion of IL-1β employs LPS-primed human peripheral blood monocytes. Subsequently, adenosine triphosphate (ATP) is added to the cells in order to trigger the P2X7 receptor resulting in processing and secretion of mature IL-1β. However, it is often reported that secretion is due to cytotoxic effects of ATP with P2X7 receptor-activation-related cell death. We have challenged this concept and demonstrate IL-1β specific secretion, since there is no increase in cell death and IL-1α and IL-18 are not released in the same cultures. More importantly we show that these conclusions can only be drawn under stringent experimental conditions. Cell Death Interleukin-1 ATP active secretion stringent experimental conditions. Immunologic diseases. Allergy Monique eStoffels verfasserin aut Ruben eZaal verfasserin aut Ruben eZaal verfasserin aut Nina eKok verfasserin aut Nina eKok verfasserin aut Jos W.M. van der Meer verfasserin aut Jos W.M. van der Meer verfasserin aut Charles A. Dinarello verfasserin aut Charles A. Dinarello verfasserin aut Anna eSimon verfasserin aut Anna eSimon verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 6(2015) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:6 year:2015 https://doi.org/10.3389/fimmu.2015.00054 kostenfrei https://doaj.org/article/26ada269dc02429ba3f6da5b6cbc9631 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00054/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
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authorswithroles_txt_mv	Monique eStoffels @@aut@@ Ruben eZaal @@aut@@ Nina eKok @@aut@@ Jos W.M. van der Meer @@aut@@ Charles A. Dinarello @@aut@@ Anna eSimon @@aut@@
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spellingShingle	Monique eStoffels misc RC581-607 misc Cell Death misc Interleukin-1 misc ATP misc active secretion misc stringent experimental conditions. misc Immunologic diseases. Allergy ATP-induced IL-1β specific secretion: true under stringent conditions.
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topic_title	RC581-607 ATP-induced IL-1β specific secretion: true under stringent conditions Cell Death Interleukin-1 ATP active secretion stringent experimental conditions
topic	misc RC581-607 misc Cell Death misc Interleukin-1 misc ATP misc active secretion misc stringent experimental conditions. misc Immunologic diseases. Allergy
topic_unstemmed	misc RC581-607 misc Cell Death misc Interleukin-1 misc ATP misc active secretion misc stringent experimental conditions. misc Immunologic diseases. Allergy
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title	ATP-induced IL-1β specific secretion: true under stringent conditions.
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title_sort	atp-induced il-1β specific secretion: true under stringent conditions
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title_auth	ATP-induced IL-1β specific secretion: true under stringent conditions.
abstract	Interleukin-1β is a potent proinflammatory cytokine, of which processing and secretion are tightly regulated. After exposure to various stimuli, mononuclear phagocytes synthesize the inactive precursor (pro-IL-1β), which is then cleaved intracellularly by caspase-1 and secreted. A widely used method for in-vitro secretion of IL-1β employs LPS-primed human peripheral blood monocytes. Subsequently, adenosine triphosphate (ATP) is added to the cells in order to trigger the P2X7 receptor resulting in processing and secretion of mature IL-1β. However, it is often reported that secretion is due to cytotoxic effects of ATP with P2X7 receptor-activation-related cell death. We have challenged this concept and demonstrate IL-1β specific secretion, since there is no increase in cell death and IL-1α and IL-18 are not released in the same cultures. More importantly we show that these conclusions can only be drawn under stringent experimental conditions.
abstractGer	Interleukin-1β is a potent proinflammatory cytokine, of which processing and secretion are tightly regulated. After exposure to various stimuli, mononuclear phagocytes synthesize the inactive precursor (pro-IL-1β), which is then cleaved intracellularly by caspase-1 and secreted. A widely used method for in-vitro secretion of IL-1β employs LPS-primed human peripheral blood monocytes. Subsequently, adenosine triphosphate (ATP) is added to the cells in order to trigger the P2X7 receptor resulting in processing and secretion of mature IL-1β. However, it is often reported that secretion is due to cytotoxic effects of ATP with P2X7 receptor-activation-related cell death. We have challenged this concept and demonstrate IL-1β specific secretion, since there is no increase in cell death and IL-1α and IL-18 are not released in the same cultures. More importantly we show that these conclusions can only be drawn under stringent experimental conditions.
abstract_unstemmed	Interleukin-1β is a potent proinflammatory cytokine, of which processing and secretion are tightly regulated. After exposure to various stimuli, mononuclear phagocytes synthesize the inactive precursor (pro-IL-1β), which is then cleaved intracellularly by caspase-1 and secreted. A widely used method for in-vitro secretion of IL-1β employs LPS-primed human peripheral blood monocytes. Subsequently, adenosine triphosphate (ATP) is added to the cells in order to trigger the P2X7 receptor resulting in processing and secretion of mature IL-1β. However, it is often reported that secretion is due to cytotoxic effects of ATP with P2X7 receptor-activation-related cell death. We have challenged this concept and demonstrate IL-1β specific secretion, since there is no increase in cell death and IL-1α and IL-18 are not released in the same cultures. More importantly we show that these conclusions can only be drawn under stringent experimental conditions.
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title_short	ATP-induced IL-1β specific secretion: true under stringent conditions.
url	https://doi.org/10.3389/fimmu.2015.00054 https://doaj.org/article/26ada269dc02429ba3f6da5b6cbc9631 http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00054/full https://doaj.org/toc/1664-3224
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ATP-induced IL-1β specific secretion: true under stringent conditions.

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