Ensemble of nucleic acid absolute quantitation modules for copy number variation detection and RNA profiling

Highly multiplexed ddPCR for sensitive nucleic acid quantitation remains challenging due to limited fluorescence channels. Here the authors report quantitative amplicon sequencing (QASeq), a PCR-based molecular barcoding NGS approach which is compatible with high multiplexing.

Gespeichert in:

Autor*in:	Lucia Ruojia Wu [verfasserIn] Peng Dai [verfasserIn] Michael Xiangjiang Wang [verfasserIn] Sherry Xi Chen [verfasserIn] Evan N. Cohen [verfasserIn] Gitanjali Jayachandran [verfasserIn] Jinny Xuemeng Zhang [verfasserIn] Angela V. Serrano [verfasserIn] Nina Guanyi Xie [verfasserIn] Naoto T. Ueno [verfasserIn] James M. Reuben [verfasserIn] Carlos H. Barcenas [verfasserIn] David Yu Zhang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Übergeordnetes Werk:	In: Nature Communications - Nature Portfolio, 2016, 13(2022), 1, Seite 9
Übergeordnetes Werk:	volume:13 ; year:2022 ; number:1 ; pages:9

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1038/s41467-022-29487-y

Katalog-ID:	DOAJ009335129

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allfieldsSound	10.1038/s41467-022-29487-y doi (DE-627)DOAJ009335129 (DE-599)DOAJ4d7f4e4784404a73a971f604e13766e8 DE-627 ger DE-627 rakwb eng Lucia Ruojia Wu verfasserin aut Ensemble of nucleic acid absolute quantitation modules for copy number variation detection and RNA profiling 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Highly multiplexed ddPCR for sensitive nucleic acid quantitation remains challenging due to limited fluorescence channels. Here the authors report quantitative amplicon sequencing (QASeq), a PCR-based molecular barcoding NGS approach which is compatible with high multiplexing. Science Q Peng Dai verfasserin aut Michael Xiangjiang Wang verfasserin aut Sherry Xi Chen verfasserin aut Evan N. Cohen verfasserin aut Gitanjali Jayachandran verfasserin aut Jinny Xuemeng Zhang verfasserin aut Angela V. Serrano verfasserin aut Nina Guanyi Xie verfasserin aut Naoto T. Ueno verfasserin aut James M. Reuben verfasserin aut Carlos H. Barcenas verfasserin aut David Yu Zhang verfasserin aut In Nature Communications Nature Portfolio, 2016 13(2022), 1, Seite 9 (DE-627)626457688 (DE-600)2553671-0 20411723 nnns volume:13 year:2022 number:1 pages:9 https://doi.org/10.1038/s41467-022-29487-y kostenfrei https://doaj.org/article/4d7f4e4784404a73a971f604e13766e8 kostenfrei https://doi.org/10.1038/s41467-022-29487-y kostenfrei https://doaj.org/toc/2041-1723 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_211 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2110 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 1 9
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author	Lucia Ruojia Wu
spellingShingle	Lucia Ruojia Wu misc Science misc Q Ensemble of nucleic acid absolute quantitation modules for copy number variation detection and RNA profiling
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topic_title	Ensemble of nucleic acid absolute quantitation modules for copy number variation detection and RNA profiling
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title	Ensemble of nucleic acid absolute quantitation modules for copy number variation detection and RNA profiling
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title_full	Ensemble of nucleic acid absolute quantitation modules for copy number variation detection and RNA profiling
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title_sort	ensemble of nucleic acid absolute quantitation modules for copy number variation detection and rna profiling
title_auth	Ensemble of nucleic acid absolute quantitation modules for copy number variation detection and RNA profiling
abstract	Highly multiplexed ddPCR for sensitive nucleic acid quantitation remains challenging due to limited fluorescence channels. Here the authors report quantitative amplicon sequencing (QASeq), a PCR-based molecular barcoding NGS approach which is compatible with high multiplexing.
abstractGer	Highly multiplexed ddPCR for sensitive nucleic acid quantitation remains challenging due to limited fluorescence channels. Here the authors report quantitative amplicon sequencing (QASeq), a PCR-based molecular barcoding NGS approach which is compatible with high multiplexing.
abstract_unstemmed	Highly multiplexed ddPCR for sensitive nucleic acid quantitation remains challenging due to limited fluorescence channels. Here the authors report quantitative amplicon sequencing (QASeq), a PCR-based molecular barcoding NGS approach which is compatible with high multiplexing.
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title_short	Ensemble of nucleic acid absolute quantitation modules for copy number variation detection and RNA profiling
url	https://doi.org/10.1038/s41467-022-29487-y https://doaj.org/article/4d7f4e4784404a73a971f604e13766e8 https://doaj.org/toc/2041-1723
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Ensemble of nucleic acid absolute quantitation modules for copy number variation detection and RNA profiling

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