Triplex Hybridization-Based Nanosystem for the Rapid Screening of Pneumocystis Pneumonia in Clinical Samples

Pneumocystis pneumonia (PcP) is a disease produced by the opportunistic infection of the fungus <i<Pneumocystis jirovecii</i<. As delayed or unsuitable treatments increase the risk of mortality, the development of rapid and accurate diagnostic tools for PcP are of great importance. Unfor...
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Autor*in:	Luis Pla [verfasserIn] Anna Aviñó [verfasserIn] Ramón Eritja [verfasserIn] Alba Ruiz-Gaitán [verfasserIn] Javier Pemán [verfasserIn] Vicente Friaza [verfasserIn] Enrique J. Calderón [verfasserIn] Elena Aznar [verfasserIn] Ramón Martínez-Máñez [verfasserIn] Sara Santiago-Felipe [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	nanoporous anodic alumina molecular gates oligonucleotides biosensor

Übergeordnetes Werk:	In: Journal of Fungi - MDPI AG, 2015, 6(2020), 4, p 292
Übergeordnetes Werk:	volume:6 ; year:2020 ; number:4, p 292

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/jof6040292

Katalog-ID:	DOAJ009546057

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allfields	10.3390/jof6040292 doi (DE-627)DOAJ009546057 (DE-599)DOAJdc2991839ec14df8928982465583495a DE-627 ger DE-627 rakwb eng QH301-705.5 Luis Pla verfasserin aut Triplex Hybridization-Based Nanosystem for the Rapid Screening of Pneumocystis Pneumonia in Clinical Samples 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Pneumocystis pneumonia (PcP) is a disease produced by the opportunistic infection of the fungus <i<Pneumocystis jirovecii</i<. As delayed or unsuitable treatments increase the risk of mortality, the development of rapid and accurate diagnostic tools for PcP are of great importance. Unfortunately, current standard methods present severe limitations and are far from adequate. In this work, a time-competitive, sensitive and selective biosensor based on DNA-gated nanomaterials for the identification of <i<P. jirovecii</i< is presented. The biosensor consists of a nanoporous anodic alumina (NAA) scaffold which pores are filled with a dye reporter and capped with specific DNA oligonucleotides. In the presence of <i<P. jirovecii</i< genomic DNA, the gated biosensor is open, and the cargo is delivered to the solution where it is monitored through fluorescence spectroscopy. The use of capping oligonucleotides able to form duplex or triplex with <i<P. jirovecii</i< DNA is studied. The final diagnostic tool shows a limit of detection (LOD) of 1 nM of target complementary DNA and does not require previous amplification steps. The method was applied to identify DNA from <i<P. jirovecii</i< in unmodified bronchoalveolar lavage, nasopharyngeal aspirates, and sputum samples in 60 min. This is a promising alternative method for the routinely diagnosis of Pneumocystis pneumonia. nanoporous anodic alumina <i<Pneumocystis jirovecii</i< molecular gates oligonucleotides biosensor Biology (General) Anna Aviñó verfasserin aut Ramón Eritja verfasserin aut Alba Ruiz-Gaitán verfasserin aut Javier Pemán verfasserin aut Vicente Friaza verfasserin aut Enrique J. Calderón verfasserin aut Elena Aznar verfasserin aut Ramón Martínez-Máñez verfasserin aut Sara Santiago-Felipe verfasserin aut In Journal of Fungi MDPI AG, 2015 6(2020), 4, p 292 (DE-627)796588538 (DE-600)2784229-0 2309608X nnns volume:6 year:2020 number:4, p 292 https://doi.org/10.3390/jof6040292 kostenfrei https://doaj.org/article/dc2991839ec14df8928982465583495a kostenfrei https://www.mdpi.com/2309-608X/6/4/292 kostenfrei https://doaj.org/toc/2309-608X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2020 4, p 292
spelling	10.3390/jof6040292 doi (DE-627)DOAJ009546057 (DE-599)DOAJdc2991839ec14df8928982465583495a DE-627 ger DE-627 rakwb eng QH301-705.5 Luis Pla verfasserin aut Triplex Hybridization-Based Nanosystem for the Rapid Screening of Pneumocystis Pneumonia in Clinical Samples 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Pneumocystis pneumonia (PcP) is a disease produced by the opportunistic infection of the fungus <i<Pneumocystis jirovecii</i<. As delayed or unsuitable treatments increase the risk of mortality, the development of rapid and accurate diagnostic tools for PcP are of great importance. Unfortunately, current standard methods present severe limitations and are far from adequate. In this work, a time-competitive, sensitive and selective biosensor based on DNA-gated nanomaterials for the identification of <i<P. jirovecii</i< is presented. The biosensor consists of a nanoporous anodic alumina (NAA) scaffold which pores are filled with a dye reporter and capped with specific DNA oligonucleotides. In the presence of <i<P. jirovecii</i< genomic DNA, the gated biosensor is open, and the cargo is delivered to the solution where it is monitored through fluorescence spectroscopy. The use of capping oligonucleotides able to form duplex or triplex with <i<P. jirovecii</i< DNA is studied. The final diagnostic tool shows a limit of detection (LOD) of 1 nM of target complementary DNA and does not require previous amplification steps. The method was applied to identify DNA from <i<P. jirovecii</i< in unmodified bronchoalveolar lavage, nasopharyngeal aspirates, and sputum samples in 60 min. This is a promising alternative method for the routinely diagnosis of Pneumocystis pneumonia. nanoporous anodic alumina <i<Pneumocystis jirovecii</i< molecular gates oligonucleotides biosensor Biology (General) Anna Aviñó verfasserin aut Ramón Eritja verfasserin aut Alba Ruiz-Gaitán verfasserin aut Javier Pemán verfasserin aut Vicente Friaza verfasserin aut Enrique J. Calderón verfasserin aut Elena Aznar verfasserin aut Ramón Martínez-Máñez verfasserin aut Sara Santiago-Felipe verfasserin aut In Journal of Fungi MDPI AG, 2015 6(2020), 4, p 292 (DE-627)796588538 (DE-600)2784229-0 2309608X nnns volume:6 year:2020 number:4, p 292 https://doi.org/10.3390/jof6040292 kostenfrei https://doaj.org/article/dc2991839ec14df8928982465583495a kostenfrei https://www.mdpi.com/2309-608X/6/4/292 kostenfrei https://doaj.org/toc/2309-608X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2020 4, p 292
allfields_unstemmed	10.3390/jof6040292 doi (DE-627)DOAJ009546057 (DE-599)DOAJdc2991839ec14df8928982465583495a DE-627 ger DE-627 rakwb eng QH301-705.5 Luis Pla verfasserin aut Triplex Hybridization-Based Nanosystem for the Rapid Screening of Pneumocystis Pneumonia in Clinical Samples 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Pneumocystis pneumonia (PcP) is a disease produced by the opportunistic infection of the fungus <i<Pneumocystis jirovecii</i<. As delayed or unsuitable treatments increase the risk of mortality, the development of rapid and accurate diagnostic tools for PcP are of great importance. Unfortunately, current standard methods present severe limitations and are far from adequate. In this work, a time-competitive, sensitive and selective biosensor based on DNA-gated nanomaterials for the identification of <i<P. jirovecii</i< is presented. The biosensor consists of a nanoporous anodic alumina (NAA) scaffold which pores are filled with a dye reporter and capped with specific DNA oligonucleotides. In the presence of <i<P. jirovecii</i< genomic DNA, the gated biosensor is open, and the cargo is delivered to the solution where it is monitored through fluorescence spectroscopy. The use of capping oligonucleotides able to form duplex or triplex with <i<P. jirovecii</i< DNA is studied. The final diagnostic tool shows a limit of detection (LOD) of 1 nM of target complementary DNA and does not require previous amplification steps. The method was applied to identify DNA from <i<P. jirovecii</i< in unmodified bronchoalveolar lavage, nasopharyngeal aspirates, and sputum samples in 60 min. This is a promising alternative method for the routinely diagnosis of Pneumocystis pneumonia. nanoporous anodic alumina <i<Pneumocystis jirovecii</i< molecular gates oligonucleotides biosensor Biology (General) Anna Aviñó verfasserin aut Ramón Eritja verfasserin aut Alba Ruiz-Gaitán verfasserin aut Javier Pemán verfasserin aut Vicente Friaza verfasserin aut Enrique J. Calderón verfasserin aut Elena Aznar verfasserin aut Ramón Martínez-Máñez verfasserin aut Sara Santiago-Felipe verfasserin aut In Journal of Fungi MDPI AG, 2015 6(2020), 4, p 292 (DE-627)796588538 (DE-600)2784229-0 2309608X nnns volume:6 year:2020 number:4, p 292 https://doi.org/10.3390/jof6040292 kostenfrei https://doaj.org/article/dc2991839ec14df8928982465583495a kostenfrei https://www.mdpi.com/2309-608X/6/4/292 kostenfrei https://doaj.org/toc/2309-608X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2020 4, p 292
allfieldsGer	10.3390/jof6040292 doi (DE-627)DOAJ009546057 (DE-599)DOAJdc2991839ec14df8928982465583495a DE-627 ger DE-627 rakwb eng QH301-705.5 Luis Pla verfasserin aut Triplex Hybridization-Based Nanosystem for the Rapid Screening of Pneumocystis Pneumonia in Clinical Samples 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Pneumocystis pneumonia (PcP) is a disease produced by the opportunistic infection of the fungus <i<Pneumocystis jirovecii</i<. As delayed or unsuitable treatments increase the risk of mortality, the development of rapid and accurate diagnostic tools for PcP are of great importance. Unfortunately, current standard methods present severe limitations and are far from adequate. In this work, a time-competitive, sensitive and selective biosensor based on DNA-gated nanomaterials for the identification of <i<P. jirovecii</i< is presented. The biosensor consists of a nanoporous anodic alumina (NAA) scaffold which pores are filled with a dye reporter and capped with specific DNA oligonucleotides. In the presence of <i<P. jirovecii</i< genomic DNA, the gated biosensor is open, and the cargo is delivered to the solution where it is monitored through fluorescence spectroscopy. The use of capping oligonucleotides able to form duplex or triplex with <i<P. jirovecii</i< DNA is studied. The final diagnostic tool shows a limit of detection (LOD) of 1 nM of target complementary DNA and does not require previous amplification steps. The method was applied to identify DNA from <i<P. jirovecii</i< in unmodified bronchoalveolar lavage, nasopharyngeal aspirates, and sputum samples in 60 min. This is a promising alternative method for the routinely diagnosis of Pneumocystis pneumonia. nanoporous anodic alumina <i<Pneumocystis jirovecii</i< molecular gates oligonucleotides biosensor Biology (General) Anna Aviñó verfasserin aut Ramón Eritja verfasserin aut Alba Ruiz-Gaitán verfasserin aut Javier Pemán verfasserin aut Vicente Friaza verfasserin aut Enrique J. Calderón verfasserin aut Elena Aznar verfasserin aut Ramón Martínez-Máñez verfasserin aut Sara Santiago-Felipe verfasserin aut In Journal of Fungi MDPI AG, 2015 6(2020), 4, p 292 (DE-627)796588538 (DE-600)2784229-0 2309608X nnns volume:6 year:2020 number:4, p 292 https://doi.org/10.3390/jof6040292 kostenfrei https://doaj.org/article/dc2991839ec14df8928982465583495a kostenfrei https://www.mdpi.com/2309-608X/6/4/292 kostenfrei https://doaj.org/toc/2309-608X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2020 4, p 292
allfieldsSound	10.3390/jof6040292 doi (DE-627)DOAJ009546057 (DE-599)DOAJdc2991839ec14df8928982465583495a DE-627 ger DE-627 rakwb eng QH301-705.5 Luis Pla verfasserin aut Triplex Hybridization-Based Nanosystem for the Rapid Screening of Pneumocystis Pneumonia in Clinical Samples 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Pneumocystis pneumonia (PcP) is a disease produced by the opportunistic infection of the fungus <i<Pneumocystis jirovecii</i<. As delayed or unsuitable treatments increase the risk of mortality, the development of rapid and accurate diagnostic tools for PcP are of great importance. Unfortunately, current standard methods present severe limitations and are far from adequate. In this work, a time-competitive, sensitive and selective biosensor based on DNA-gated nanomaterials for the identification of <i<P. jirovecii</i< is presented. The biosensor consists of a nanoporous anodic alumina (NAA) scaffold which pores are filled with a dye reporter and capped with specific DNA oligonucleotides. In the presence of <i<P. jirovecii</i< genomic DNA, the gated biosensor is open, and the cargo is delivered to the solution where it is monitored through fluorescence spectroscopy. The use of capping oligonucleotides able to form duplex or triplex with <i<P. jirovecii</i< DNA is studied. The final diagnostic tool shows a limit of detection (LOD) of 1 nM of target complementary DNA and does not require previous amplification steps. The method was applied to identify DNA from <i<P. jirovecii</i< in unmodified bronchoalveolar lavage, nasopharyngeal aspirates, and sputum samples in 60 min. This is a promising alternative method for the routinely diagnosis of Pneumocystis pneumonia. nanoporous anodic alumina <i<Pneumocystis jirovecii</i< molecular gates oligonucleotides biosensor Biology (General) Anna Aviñó verfasserin aut Ramón Eritja verfasserin aut Alba Ruiz-Gaitán verfasserin aut Javier Pemán verfasserin aut Vicente Friaza verfasserin aut Enrique J. Calderón verfasserin aut Elena Aznar verfasserin aut Ramón Martínez-Máñez verfasserin aut Sara Santiago-Felipe verfasserin aut In Journal of Fungi MDPI AG, 2015 6(2020), 4, p 292 (DE-627)796588538 (DE-600)2784229-0 2309608X nnns volume:6 year:2020 number:4, p 292 https://doi.org/10.3390/jof6040292 kostenfrei https://doaj.org/article/dc2991839ec14df8928982465583495a kostenfrei https://www.mdpi.com/2309-608X/6/4/292 kostenfrei https://doaj.org/toc/2309-608X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2020 4, p 292
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author	Luis Pla
spellingShingle	Luis Pla misc QH301-705.5 misc nanoporous anodic alumina misc <i<Pneumocystis jirovecii</i< misc molecular gates misc oligonucleotides misc biosensor misc Biology (General) Triplex Hybridization-Based Nanosystem for the Rapid Screening of Pneumocystis Pneumonia in Clinical Samples
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topic_title	QH301-705.5 Triplex Hybridization-Based Nanosystem for the Rapid Screening of Pneumocystis Pneumonia in Clinical Samples nanoporous anodic alumina <i<Pneumocystis jirovecii</i< molecular gates oligonucleotides biosensor
topic	misc QH301-705.5 misc nanoporous anodic alumina misc <i<Pneumocystis jirovecii</i< misc molecular gates misc oligonucleotides misc biosensor misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc nanoporous anodic alumina misc <i<Pneumocystis jirovecii</i< misc molecular gates misc oligonucleotides misc biosensor misc Biology (General)
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title	Triplex Hybridization-Based Nanosystem for the Rapid Screening of Pneumocystis Pneumonia in Clinical Samples
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title_full	Triplex Hybridization-Based Nanosystem for the Rapid Screening of Pneumocystis Pneumonia in Clinical Samples
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title_sort	triplex hybridization-based nanosystem for the rapid screening of pneumocystis pneumonia in clinical samples
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title_auth	Triplex Hybridization-Based Nanosystem for the Rapid Screening of Pneumocystis Pneumonia in Clinical Samples
abstract	Pneumocystis pneumonia (PcP) is a disease produced by the opportunistic infection of the fungus <i<Pneumocystis jirovecii</i<. As delayed or unsuitable treatments increase the risk of mortality, the development of rapid and accurate diagnostic tools for PcP are of great importance. Unfortunately, current standard methods present severe limitations and are far from adequate. In this work, a time-competitive, sensitive and selective biosensor based on DNA-gated nanomaterials for the identification of <i<P. jirovecii</i< is presented. The biosensor consists of a nanoporous anodic alumina (NAA) scaffold which pores are filled with a dye reporter and capped with specific DNA oligonucleotides. In the presence of <i<P. jirovecii</i< genomic DNA, the gated biosensor is open, and the cargo is delivered to the solution where it is monitored through fluorescence spectroscopy. The use of capping oligonucleotides able to form duplex or triplex with <i<P. jirovecii</i< DNA is studied. The final diagnostic tool shows a limit of detection (LOD) of 1 nM of target complementary DNA and does not require previous amplification steps. The method was applied to identify DNA from <i<P. jirovecii</i< in unmodified bronchoalveolar lavage, nasopharyngeal aspirates, and sputum samples in 60 min. This is a promising alternative method for the routinely diagnosis of Pneumocystis pneumonia.
abstractGer	Pneumocystis pneumonia (PcP) is a disease produced by the opportunistic infection of the fungus <i<Pneumocystis jirovecii</i<. As delayed or unsuitable treatments increase the risk of mortality, the development of rapid and accurate diagnostic tools for PcP are of great importance. Unfortunately, current standard methods present severe limitations and are far from adequate. In this work, a time-competitive, sensitive and selective biosensor based on DNA-gated nanomaterials for the identification of <i<P. jirovecii</i< is presented. The biosensor consists of a nanoporous anodic alumina (NAA) scaffold which pores are filled with a dye reporter and capped with specific DNA oligonucleotides. In the presence of <i<P. jirovecii</i< genomic DNA, the gated biosensor is open, and the cargo is delivered to the solution where it is monitored through fluorescence spectroscopy. The use of capping oligonucleotides able to form duplex or triplex with <i<P. jirovecii</i< DNA is studied. The final diagnostic tool shows a limit of detection (LOD) of 1 nM of target complementary DNA and does not require previous amplification steps. The method was applied to identify DNA from <i<P. jirovecii</i< in unmodified bronchoalveolar lavage, nasopharyngeal aspirates, and sputum samples in 60 min. This is a promising alternative method for the routinely diagnosis of Pneumocystis pneumonia.
abstract_unstemmed	Pneumocystis pneumonia (PcP) is a disease produced by the opportunistic infection of the fungus <i<Pneumocystis jirovecii</i<. As delayed or unsuitable treatments increase the risk of mortality, the development of rapid and accurate diagnostic tools for PcP are of great importance. Unfortunately, current standard methods present severe limitations and are far from adequate. In this work, a time-competitive, sensitive and selective biosensor based on DNA-gated nanomaterials for the identification of <i<P. jirovecii</i< is presented. The biosensor consists of a nanoporous anodic alumina (NAA) scaffold which pores are filled with a dye reporter and capped with specific DNA oligonucleotides. In the presence of <i<P. jirovecii</i< genomic DNA, the gated biosensor is open, and the cargo is delivered to the solution where it is monitored through fluorescence spectroscopy. The use of capping oligonucleotides able to form duplex or triplex with <i<P. jirovecii</i< DNA is studied. The final diagnostic tool shows a limit of detection (LOD) of 1 nM of target complementary DNA and does not require previous amplification steps. The method was applied to identify DNA from <i<P. jirovecii</i< in unmodified bronchoalveolar lavage, nasopharyngeal aspirates, and sputum samples in 60 min. This is a promising alternative method for the routinely diagnosis of Pneumocystis pneumonia.
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container_issue	4, p 292
title_short	Triplex Hybridization-Based Nanosystem for the Rapid Screening of Pneumocystis Pneumonia in Clinical Samples
url	https://doi.org/10.3390/jof6040292 https://doaj.org/article/dc2991839ec14df8928982465583495a https://www.mdpi.com/2309-608X/6/4/292 https://doaj.org/toc/2309-608X
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author2	Anna Aviñó Ramón Eritja Alba Ruiz-Gaitán Javier Pemán Vicente Friaza Enrique J. Calderón Elena Aznar Ramón Martínez-Máñez Sara Santiago-Felipe
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As delayed or unsuitable treatments increase the risk of mortality, the development of rapid and accurate diagnostic tools for PcP are of great importance. Unfortunately, current standard methods present severe limitations and are far from adequate. In this work, a time-competitive, sensitive and selective biosensor based on DNA-gated nanomaterials for the identification of <i<P. jirovecii</i< is presented. The biosensor consists of a nanoporous anodic alumina (NAA) scaffold which pores are filled with a dye reporter and capped with specific DNA oligonucleotides. In the presence of <i<P. jirovecii</i< genomic DNA, the gated biosensor is open, and the cargo is delivered to the solution where it is monitored through fluorescence spectroscopy. The use of capping oligonucleotides able to form duplex or triplex with <i<P. jirovecii</i< DNA is studied. The final diagnostic tool shows a limit of detection (LOD) of 1 nM of target complementary DNA and does not require previous amplification steps. The method was applied to identify DNA from <i<P. jirovecii</i< in unmodified bronchoalveolar lavage, nasopharyngeal aspirates, and sputum samples in 60 min. 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Triplex Hybridization-Based Nanosystem for the Rapid Screening of Pneumocystis Pneumonia in Clinical Samples

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