In Vitro-Reconstituted Nucleoids Can Block Mitochondrial DNA Replication and Transcription

The mechanisms regulating the number of active copies of mtDNA are still unclear. A mammalian cell typically contains 1,000–10,000 copies of mtDNA, which are packaged into nucleoprotein complexes termed nucleoids. The main protein component of these structures is mitochondrial transcription factor A...
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Autor*in:	Géraldine Farge [verfasserIn] Majda Mehmedovic [verfasserIn] Marian Baclayon [verfasserIn] Siet M.J.L. van den Wildenberg [verfasserIn] Wouter H. Roos [verfasserIn] Claes M. Gustafsson [verfasserIn] Gijs J.L. Wuite [verfasserIn] Maria Falkenberg [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2014

Übergeordnetes Werk:	In: Cell Reports - Elsevier, 2015, 8(2014), 1, Seite 66-74
Übergeordnetes Werk:	volume:8 ; year:2014 ; number:1 ; pages:66-74

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.celrep.2014.05.046

Katalog-ID:	DOAJ009577394

Internformat


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520			\|a The mechanisms regulating the number of active copies of mtDNA are still unclear. A mammalian cell typically contains 1,000–10,000 copies of mtDNA, which are packaged into nucleoprotein complexes termed nucleoids. The main protein component of these structures is mitochondrial transcription factor A (TFAM). Here, we reconstitute nucleoid-like particles in vitro and demonstrate that small changes in TFAM levels dramatically impact the fraction of DNA molecules available for transcription and DNA replication. Compaction by TFAM is highly cooperative, and at physiological ratios of TFAM to DNA, there are large variations in compaction, from fully compacted nucleoids to naked DNA. In compacted nucleoids, TFAM forms stable protein filaments on DNA that block melting and prevent progression of the replication and transcription machineries. Based on our observations, we suggest that small variations in the TFAM-to-mtDNA ratio may be used to regulate mitochondrial gene transcription and DNA replication.
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publishDate	2014
allfields	10.1016/j.celrep.2014.05.046 doi (DE-627)DOAJ009577394 (DE-599)DOAJ4acdd44cefd14979a254ad7905243bc2 DE-627 ger DE-627 rakwb eng QH301-705.5 Géraldine Farge verfasserin aut In Vitro-Reconstituted Nucleoids Can Block Mitochondrial DNA Replication and Transcription 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The mechanisms regulating the number of active copies of mtDNA are still unclear. A mammalian cell typically contains 1,000–10,000 copies of mtDNA, which are packaged into nucleoprotein complexes termed nucleoids. The main protein component of these structures is mitochondrial transcription factor A (TFAM). Here, we reconstitute nucleoid-like particles in vitro and demonstrate that small changes in TFAM levels dramatically impact the fraction of DNA molecules available for transcription and DNA replication. Compaction by TFAM is highly cooperative, and at physiological ratios of TFAM to DNA, there are large variations in compaction, from fully compacted nucleoids to naked DNA. In compacted nucleoids, TFAM forms stable protein filaments on DNA that block melting and prevent progression of the replication and transcription machineries. Based on our observations, we suggest that small variations in the TFAM-to-mtDNA ratio may be used to regulate mitochondrial gene transcription and DNA replication. Biology (General) Majda Mehmedovic verfasserin aut Marian Baclayon verfasserin aut Siet M.J.L. van den Wildenberg verfasserin aut Wouter H. Roos verfasserin aut Claes M. Gustafsson verfasserin aut Gijs J.L. Wuite verfasserin aut Maria Falkenberg verfasserin aut In Cell Reports Elsevier, 2015 8(2014), 1, Seite 66-74 (DE-627)684964562 (DE-600)2649101-1 22111247 nnns volume:8 year:2014 number:1 pages:66-74 https://doi.org/10.1016/j.celrep.2014.05.046 kostenfrei https://doaj.org/article/4acdd44cefd14979a254ad7905243bc2 kostenfrei http://www.sciencedirect.com/science/article/pii/S2211124714004409 kostenfrei https://doaj.org/toc/2211-1247 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 8 2014 1 66-74
spelling	10.1016/j.celrep.2014.05.046 doi (DE-627)DOAJ009577394 (DE-599)DOAJ4acdd44cefd14979a254ad7905243bc2 DE-627 ger DE-627 rakwb eng QH301-705.5 Géraldine Farge verfasserin aut In Vitro-Reconstituted Nucleoids Can Block Mitochondrial DNA Replication and Transcription 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The mechanisms regulating the number of active copies of mtDNA are still unclear. A mammalian cell typically contains 1,000–10,000 copies of mtDNA, which are packaged into nucleoprotein complexes termed nucleoids. The main protein component of these structures is mitochondrial transcription factor A (TFAM). Here, we reconstitute nucleoid-like particles in vitro and demonstrate that small changes in TFAM levels dramatically impact the fraction of DNA molecules available for transcription and DNA replication. Compaction by TFAM is highly cooperative, and at physiological ratios of TFAM to DNA, there are large variations in compaction, from fully compacted nucleoids to naked DNA. In compacted nucleoids, TFAM forms stable protein filaments on DNA that block melting and prevent progression of the replication and transcription machineries. Based on our observations, we suggest that small variations in the TFAM-to-mtDNA ratio may be used to regulate mitochondrial gene transcription and DNA replication. Biology (General) Majda Mehmedovic verfasserin aut Marian Baclayon verfasserin aut Siet M.J.L. van den Wildenberg verfasserin aut Wouter H. Roos verfasserin aut Claes M. Gustafsson verfasserin aut Gijs J.L. Wuite verfasserin aut Maria Falkenberg verfasserin aut In Cell Reports Elsevier, 2015 8(2014), 1, Seite 66-74 (DE-627)684964562 (DE-600)2649101-1 22111247 nnns volume:8 year:2014 number:1 pages:66-74 https://doi.org/10.1016/j.celrep.2014.05.046 kostenfrei https://doaj.org/article/4acdd44cefd14979a254ad7905243bc2 kostenfrei http://www.sciencedirect.com/science/article/pii/S2211124714004409 kostenfrei https://doaj.org/toc/2211-1247 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 8 2014 1 66-74
allfields_unstemmed	10.1016/j.celrep.2014.05.046 doi (DE-627)DOAJ009577394 (DE-599)DOAJ4acdd44cefd14979a254ad7905243bc2 DE-627 ger DE-627 rakwb eng QH301-705.5 Géraldine Farge verfasserin aut In Vitro-Reconstituted Nucleoids Can Block Mitochondrial DNA Replication and Transcription 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The mechanisms regulating the number of active copies of mtDNA are still unclear. A mammalian cell typically contains 1,000–10,000 copies of mtDNA, which are packaged into nucleoprotein complexes termed nucleoids. The main protein component of these structures is mitochondrial transcription factor A (TFAM). Here, we reconstitute nucleoid-like particles in vitro and demonstrate that small changes in TFAM levels dramatically impact the fraction of DNA molecules available for transcription and DNA replication. Compaction by TFAM is highly cooperative, and at physiological ratios of TFAM to DNA, there are large variations in compaction, from fully compacted nucleoids to naked DNA. In compacted nucleoids, TFAM forms stable protein filaments on DNA that block melting and prevent progression of the replication and transcription machineries. Based on our observations, we suggest that small variations in the TFAM-to-mtDNA ratio may be used to regulate mitochondrial gene transcription and DNA replication. Biology (General) Majda Mehmedovic verfasserin aut Marian Baclayon verfasserin aut Siet M.J.L. van den Wildenberg verfasserin aut Wouter H. Roos verfasserin aut Claes M. Gustafsson verfasserin aut Gijs J.L. Wuite verfasserin aut Maria Falkenberg verfasserin aut In Cell Reports Elsevier, 2015 8(2014), 1, Seite 66-74 (DE-627)684964562 (DE-600)2649101-1 22111247 nnns volume:8 year:2014 number:1 pages:66-74 https://doi.org/10.1016/j.celrep.2014.05.046 kostenfrei https://doaj.org/article/4acdd44cefd14979a254ad7905243bc2 kostenfrei http://www.sciencedirect.com/science/article/pii/S2211124714004409 kostenfrei https://doaj.org/toc/2211-1247 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 8 2014 1 66-74
allfieldsGer	10.1016/j.celrep.2014.05.046 doi (DE-627)DOAJ009577394 (DE-599)DOAJ4acdd44cefd14979a254ad7905243bc2 DE-627 ger DE-627 rakwb eng QH301-705.5 Géraldine Farge verfasserin aut In Vitro-Reconstituted Nucleoids Can Block Mitochondrial DNA Replication and Transcription 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The mechanisms regulating the number of active copies of mtDNA are still unclear. A mammalian cell typically contains 1,000–10,000 copies of mtDNA, which are packaged into nucleoprotein complexes termed nucleoids. The main protein component of these structures is mitochondrial transcription factor A (TFAM). Here, we reconstitute nucleoid-like particles in vitro and demonstrate that small changes in TFAM levels dramatically impact the fraction of DNA molecules available for transcription and DNA replication. Compaction by TFAM is highly cooperative, and at physiological ratios of TFAM to DNA, there are large variations in compaction, from fully compacted nucleoids to naked DNA. In compacted nucleoids, TFAM forms stable protein filaments on DNA that block melting and prevent progression of the replication and transcription machineries. Based on our observations, we suggest that small variations in the TFAM-to-mtDNA ratio may be used to regulate mitochondrial gene transcription and DNA replication. Biology (General) Majda Mehmedovic verfasserin aut Marian Baclayon verfasserin aut Siet M.J.L. van den Wildenberg verfasserin aut Wouter H. Roos verfasserin aut Claes M. Gustafsson verfasserin aut Gijs J.L. Wuite verfasserin aut Maria Falkenberg verfasserin aut In Cell Reports Elsevier, 2015 8(2014), 1, Seite 66-74 (DE-627)684964562 (DE-600)2649101-1 22111247 nnns volume:8 year:2014 number:1 pages:66-74 https://doi.org/10.1016/j.celrep.2014.05.046 kostenfrei https://doaj.org/article/4acdd44cefd14979a254ad7905243bc2 kostenfrei http://www.sciencedirect.com/science/article/pii/S2211124714004409 kostenfrei https://doaj.org/toc/2211-1247 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 8 2014 1 66-74
allfieldsSound	10.1016/j.celrep.2014.05.046 doi (DE-627)DOAJ009577394 (DE-599)DOAJ4acdd44cefd14979a254ad7905243bc2 DE-627 ger DE-627 rakwb eng QH301-705.5 Géraldine Farge verfasserin aut In Vitro-Reconstituted Nucleoids Can Block Mitochondrial DNA Replication and Transcription 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The mechanisms regulating the number of active copies of mtDNA are still unclear. A mammalian cell typically contains 1,000–10,000 copies of mtDNA, which are packaged into nucleoprotein complexes termed nucleoids. The main protein component of these structures is mitochondrial transcription factor A (TFAM). Here, we reconstitute nucleoid-like particles in vitro and demonstrate that small changes in TFAM levels dramatically impact the fraction of DNA molecules available for transcription and DNA replication. Compaction by TFAM is highly cooperative, and at physiological ratios of TFAM to DNA, there are large variations in compaction, from fully compacted nucleoids to naked DNA. In compacted nucleoids, TFAM forms stable protein filaments on DNA that block melting and prevent progression of the replication and transcription machineries. Based on our observations, we suggest that small variations in the TFAM-to-mtDNA ratio may be used to regulate mitochondrial gene transcription and DNA replication. Biology (General) Majda Mehmedovic verfasserin aut Marian Baclayon verfasserin aut Siet M.J.L. van den Wildenberg verfasserin aut Wouter H. Roos verfasserin aut Claes M. Gustafsson verfasserin aut Gijs J.L. Wuite verfasserin aut Maria Falkenberg verfasserin aut In Cell Reports Elsevier, 2015 8(2014), 1, Seite 66-74 (DE-627)684964562 (DE-600)2649101-1 22111247 nnns volume:8 year:2014 number:1 pages:66-74 https://doi.org/10.1016/j.celrep.2014.05.046 kostenfrei https://doaj.org/article/4acdd44cefd14979a254ad7905243bc2 kostenfrei http://www.sciencedirect.com/science/article/pii/S2211124714004409 kostenfrei https://doaj.org/toc/2211-1247 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 8 2014 1 66-74
language	English
source	In Cell Reports 8(2014), 1, Seite 66-74 volume:8 year:2014 number:1 pages:66-74
sourceStr	In Cell Reports 8(2014), 1, Seite 66-74 volume:8 year:2014 number:1 pages:66-74
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Biology (General)
isfreeaccess_bool	true
container_title	Cell Reports
authorswithroles_txt_mv	Géraldine Farge @@aut@@ Majda Mehmedovic @@aut@@ Marian Baclayon @@aut@@ Siet M.J.L. van den Wildenberg @@aut@@ Wouter H. Roos @@aut@@ Claes M. Gustafsson @@aut@@ Gijs J.L. Wuite @@aut@@ Maria Falkenberg @@aut@@
publishDateDaySort_date	2014-01-01T00:00:00Z
hierarchy_top_id	684964562
id	DOAJ009577394
language_de	englisch
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callnumber-first	Q - Science
author	Géraldine Farge
spellingShingle	Géraldine Farge misc QH301-705.5 misc Biology (General) In Vitro-Reconstituted Nucleoids Can Block Mitochondrial DNA Replication and Transcription
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topic_title	QH301-705.5 In Vitro-Reconstituted Nucleoids Can Block Mitochondrial DNA Replication and Transcription
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title	In Vitro-Reconstituted Nucleoids Can Block Mitochondrial DNA Replication and Transcription
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title_full	In Vitro-Reconstituted Nucleoids Can Block Mitochondrial DNA Replication and Transcription
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author_browse	Géraldine Farge Majda Mehmedovic Marian Baclayon Siet M.J.L. van den Wildenberg Wouter H. Roos Claes M. Gustafsson Gijs J.L. Wuite Maria Falkenberg
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author-letter	Géraldine Farge
doi_str_mv	10.1016/j.celrep.2014.05.046
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title_sort	in vitro-reconstituted nucleoids can block mitochondrial dna replication and transcription
callnumber	QH301-705.5
title_auth	In Vitro-Reconstituted Nucleoids Can Block Mitochondrial DNA Replication and Transcription
abstract	The mechanisms regulating the number of active copies of mtDNA are still unclear. A mammalian cell typically contains 1,000–10,000 copies of mtDNA, which are packaged into nucleoprotein complexes termed nucleoids. The main protein component of these structures is mitochondrial transcription factor A (TFAM). Here, we reconstitute nucleoid-like particles in vitro and demonstrate that small changes in TFAM levels dramatically impact the fraction of DNA molecules available for transcription and DNA replication. Compaction by TFAM is highly cooperative, and at physiological ratios of TFAM to DNA, there are large variations in compaction, from fully compacted nucleoids to naked DNA. In compacted nucleoids, TFAM forms stable protein filaments on DNA that block melting and prevent progression of the replication and transcription machineries. Based on our observations, we suggest that small variations in the TFAM-to-mtDNA ratio may be used to regulate mitochondrial gene transcription and DNA replication.
abstractGer	The mechanisms regulating the number of active copies of mtDNA are still unclear. A mammalian cell typically contains 1,000–10,000 copies of mtDNA, which are packaged into nucleoprotein complexes termed nucleoids. The main protein component of these structures is mitochondrial transcription factor A (TFAM). Here, we reconstitute nucleoid-like particles in vitro and demonstrate that small changes in TFAM levels dramatically impact the fraction of DNA molecules available for transcription and DNA replication. Compaction by TFAM is highly cooperative, and at physiological ratios of TFAM to DNA, there are large variations in compaction, from fully compacted nucleoids to naked DNA. In compacted nucleoids, TFAM forms stable protein filaments on DNA that block melting and prevent progression of the replication and transcription machineries. Based on our observations, we suggest that small variations in the TFAM-to-mtDNA ratio may be used to regulate mitochondrial gene transcription and DNA replication.
abstract_unstemmed	The mechanisms regulating the number of active copies of mtDNA are still unclear. A mammalian cell typically contains 1,000–10,000 copies of mtDNA, which are packaged into nucleoprotein complexes termed nucleoids. The main protein component of these structures is mitochondrial transcription factor A (TFAM). Here, we reconstitute nucleoid-like particles in vitro and demonstrate that small changes in TFAM levels dramatically impact the fraction of DNA molecules available for transcription and DNA replication. Compaction by TFAM is highly cooperative, and at physiological ratios of TFAM to DNA, there are large variations in compaction, from fully compacted nucleoids to naked DNA. In compacted nucleoids, TFAM forms stable protein filaments on DNA that block melting and prevent progression of the replication and transcription machineries. Based on our observations, we suggest that small variations in the TFAM-to-mtDNA ratio may be used to regulate mitochondrial gene transcription and DNA replication.
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title_short	In Vitro-Reconstituted Nucleoids Can Block Mitochondrial DNA Replication and Transcription
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up_date	2024-07-04T00:12:59.715Z
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