Osthole Enhances Osteogenesis in Osteoblasts by Elevating Transcription Factor Osterix via cAMP/CREB Signaling In Vitro and In Vivo

Anabolic anti-osteoporotic agents are desirable for treatment and prevention of osteoporosis and fragility fractures. Osthole is a coumarin derivative extracted from the medicinal herbs Cnidium monnieri (L.) Cusson and Angelica pubescens Maxim.f. Osthole has been reported with osteogenic and anti-os...
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Gespeichert in:

Autor*in:	Zhong-Rong Zhang [verfasserIn] Wing Nang Leung [verfasserIn] Gang Li [verfasserIn] Siu Kai Kong [verfasserIn] Xiong Lu [verfasserIn] Yin Mei Wong [verfasserIn] Chun Wai Chan [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	osthole osteoblast osteogenesis bone regeneration fracture repair cAMP/CREB signaling

Übergeordnetes Werk:	In: Nutrients - MDPI AG, 2009, 9(2017), 6, p 588
Übergeordnetes Werk:	volume:9 ; year:2017 ; number:6, p 588

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/nu9060588

Katalog-ID:	DOAJ009656537

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allfieldsSound	10.3390/nu9060588 doi (DE-627)DOAJ009656537 (DE-599)DOAJ1cea2d575bfb4928a7c852d4756dd8ab DE-627 ger DE-627 rakwb eng TX341-641 Zhong-Rong Zhang verfasserin aut Osthole Enhances Osteogenesis in Osteoblasts by Elevating Transcription Factor Osterix via cAMP/CREB Signaling In Vitro and In Vivo 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Anabolic anti-osteoporotic agents are desirable for treatment and prevention of osteoporosis and fragility fractures. Osthole is a coumarin derivative extracted from the medicinal herbs Cnidium monnieri (L.) Cusson and Angelica pubescens Maxim.f. Osthole has been reported with osteogenic and anti-osteoporotic properties, whereas the underlying mechanism of its benefit still remains unclear. The objective of the present study was to investigate the osteopromotive action of osthole on mouse osteoblastic MC3T3-E1 cells and on mouse femoral fracture repair, and to explore the interaction between osthole-induced osteopromotive effect and cyclic adenosine monophosphate (cAMP) elevating effect. Osthole treatment promoted osteogenesis in osteoblasts by enhancing alkaline phosphatase (ALP) activity and mineralization. Oral gavage of osthole enhanced fracture repair and increased bone strength. Mechanistic study showed osthole triggered the cAMP/CREB pathway through the elevation of the intracellular cAMP level and activation of the phosphorylation of the cAMP response element-binding protein (CREB). Blockage of cAMP/CREB downstream signals with protein kinase A (PKA) inhibitor KT5720 partially suppressed osthole-mediated osteogenesis by inhibiting the elevation of transcription factor, osterix. In conclusion, osthole shows osteopromotive effect on osteoblasts in vitro and in vivo. Osthole-mediated osteogenesis is related to activation of the cAMP/CREB signaling pathway and downstream osterix expression. osthole osteoblast osteogenesis bone regeneration fracture repair cAMP/CREB signaling Nutrition. Foods and food supply Wing Nang Leung verfasserin aut Gang Li verfasserin aut Siu Kai Kong verfasserin aut Xiong Lu verfasserin aut Yin Mei Wong verfasserin aut Chun Wai Chan verfasserin aut In Nutrients MDPI AG, 2009 9(2017), 6, p 588 (DE-627)610604155 (DE-600)2518386-2 20726643 nnns volume:9 year:2017 number:6, p 588 https://doi.org/10.3390/nu9060588 kostenfrei https://doaj.org/article/1cea2d575bfb4928a7c852d4756dd8ab kostenfrei http://www.mdpi.com/2072-6643/9/6/588 kostenfrei https://doaj.org/toc/2072-6643 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2017 6, p 588
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topic_facet	osthole osteoblast osteogenesis bone regeneration fracture repair cAMP/CREB signaling Nutrition. Foods and food supply
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authorswithroles_txt_mv	Zhong-Rong Zhang @@aut@@ Wing Nang Leung @@aut@@ Gang Li @@aut@@ Siu Kai Kong @@aut@@ Xiong Lu @@aut@@ Yin Mei Wong @@aut@@ Chun Wai Chan @@aut@@
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callnumber-first	T - Technology
author	Zhong-Rong Zhang
spellingShingle	Zhong-Rong Zhang misc TX341-641 misc osthole misc osteoblast misc osteogenesis misc bone regeneration misc fracture repair misc cAMP/CREB signaling misc Nutrition. Foods and food supply Osthole Enhances Osteogenesis in Osteoblasts by Elevating Transcription Factor Osterix via cAMP/CREB Signaling In Vitro and In Vivo
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topic_title	TX341-641 Osthole Enhances Osteogenesis in Osteoblasts by Elevating Transcription Factor Osterix via cAMP/CREB Signaling In Vitro and In Vivo osthole osteoblast osteogenesis bone regeneration fracture repair cAMP/CREB signaling
topic	misc TX341-641 misc osthole misc osteoblast misc osteogenesis misc bone regeneration misc fracture repair misc cAMP/CREB signaling misc Nutrition. Foods and food supply
topic_unstemmed	misc TX341-641 misc osthole misc osteoblast misc osteogenesis misc bone regeneration misc fracture repair misc cAMP/CREB signaling misc Nutrition. Foods and food supply
topic_browse	misc TX341-641 misc osthole misc osteoblast misc osteogenesis misc bone regeneration misc fracture repair misc cAMP/CREB signaling misc Nutrition. Foods and food supply
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title	Osthole Enhances Osteogenesis in Osteoblasts by Elevating Transcription Factor Osterix via cAMP/CREB Signaling In Vitro and In Vivo
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title_full	Osthole Enhances Osteogenesis in Osteoblasts by Elevating Transcription Factor Osterix via cAMP/CREB Signaling In Vitro and In Vivo
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author_browse	Zhong-Rong Zhang Wing Nang Leung Gang Li Siu Kai Kong Xiong Lu Yin Mei Wong Chun Wai Chan
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title_sort	osthole enhances osteogenesis in osteoblasts by elevating transcription factor osterix via camp/creb signaling in vitro and in vivo
callnumber	TX341-641
title_auth	Osthole Enhances Osteogenesis in Osteoblasts by Elevating Transcription Factor Osterix via cAMP/CREB Signaling In Vitro and In Vivo
abstract	Anabolic anti-osteoporotic agents are desirable for treatment and prevention of osteoporosis and fragility fractures. Osthole is a coumarin derivative extracted from the medicinal herbs Cnidium monnieri (L.) Cusson and Angelica pubescens Maxim.f. Osthole has been reported with osteogenic and anti-osteoporotic properties, whereas the underlying mechanism of its benefit still remains unclear. The objective of the present study was to investigate the osteopromotive action of osthole on mouse osteoblastic MC3T3-E1 cells and on mouse femoral fracture repair, and to explore the interaction between osthole-induced osteopromotive effect and cyclic adenosine monophosphate (cAMP) elevating effect. Osthole treatment promoted osteogenesis in osteoblasts by enhancing alkaline phosphatase (ALP) activity and mineralization. Oral gavage of osthole enhanced fracture repair and increased bone strength. Mechanistic study showed osthole triggered the cAMP/CREB pathway through the elevation of the intracellular cAMP level and activation of the phosphorylation of the cAMP response element-binding protein (CREB). Blockage of cAMP/CREB downstream signals with protein kinase A (PKA) inhibitor KT5720 partially suppressed osthole-mediated osteogenesis by inhibiting the elevation of transcription factor, osterix. In conclusion, osthole shows osteopromotive effect on osteoblasts in vitro and in vivo. Osthole-mediated osteogenesis is related to activation of the cAMP/CREB signaling pathway and downstream osterix expression.
abstractGer	Anabolic anti-osteoporotic agents are desirable for treatment and prevention of osteoporosis and fragility fractures. Osthole is a coumarin derivative extracted from the medicinal herbs Cnidium monnieri (L.) Cusson and Angelica pubescens Maxim.f. Osthole has been reported with osteogenic and anti-osteoporotic properties, whereas the underlying mechanism of its benefit still remains unclear. The objective of the present study was to investigate the osteopromotive action of osthole on mouse osteoblastic MC3T3-E1 cells and on mouse femoral fracture repair, and to explore the interaction between osthole-induced osteopromotive effect and cyclic adenosine monophosphate (cAMP) elevating effect. Osthole treatment promoted osteogenesis in osteoblasts by enhancing alkaline phosphatase (ALP) activity and mineralization. Oral gavage of osthole enhanced fracture repair and increased bone strength. Mechanistic study showed osthole triggered the cAMP/CREB pathway through the elevation of the intracellular cAMP level and activation of the phosphorylation of the cAMP response element-binding protein (CREB). Blockage of cAMP/CREB downstream signals with protein kinase A (PKA) inhibitor KT5720 partially suppressed osthole-mediated osteogenesis by inhibiting the elevation of transcription factor, osterix. In conclusion, osthole shows osteopromotive effect on osteoblasts in vitro and in vivo. Osthole-mediated osteogenesis is related to activation of the cAMP/CREB signaling pathway and downstream osterix expression.
abstract_unstemmed	Anabolic anti-osteoporotic agents are desirable for treatment and prevention of osteoporosis and fragility fractures. Osthole is a coumarin derivative extracted from the medicinal herbs Cnidium monnieri (L.) Cusson and Angelica pubescens Maxim.f. Osthole has been reported with osteogenic and anti-osteoporotic properties, whereas the underlying mechanism of its benefit still remains unclear. The objective of the present study was to investigate the osteopromotive action of osthole on mouse osteoblastic MC3T3-E1 cells and on mouse femoral fracture repair, and to explore the interaction between osthole-induced osteopromotive effect and cyclic adenosine monophosphate (cAMP) elevating effect. Osthole treatment promoted osteogenesis in osteoblasts by enhancing alkaline phosphatase (ALP) activity and mineralization. Oral gavage of osthole enhanced fracture repair and increased bone strength. Mechanistic study showed osthole triggered the cAMP/CREB pathway through the elevation of the intracellular cAMP level and activation of the phosphorylation of the cAMP response element-binding protein (CREB). Blockage of cAMP/CREB downstream signals with protein kinase A (PKA) inhibitor KT5720 partially suppressed osthole-mediated osteogenesis by inhibiting the elevation of transcription factor, osterix. In conclusion, osthole shows osteopromotive effect on osteoblasts in vitro and in vivo. Osthole-mediated osteogenesis is related to activation of the cAMP/CREB signaling pathway and downstream osterix expression.
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container_issue	6, p 588
title_short	Osthole Enhances Osteogenesis in Osteoblasts by Elevating Transcription Factor Osterix via cAMP/CREB Signaling In Vitro and In Vivo
url	https://doi.org/10.3390/nu9060588 https://doaj.org/article/1cea2d575bfb4928a7c852d4756dd8ab http://www.mdpi.com/2072-6643/9/6/588 https://doaj.org/toc/2072-6643
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up_date	2024-07-04T00:31:51.941Z
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Osthole Enhances Osteogenesis in Osteoblasts by Elevating Transcription Factor Osterix via cAMP/CREB Signaling In Vitro and In Vivo

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