Global identification of genes and pathways regulated by Akt during activation of T helper cells [v1; ref status: indexed, http://f1000r.es/107]

We previously demonstrated that Akt differentially modulated a subset of NF-kB target genes during T cell activation. In the current study, we further explored the broader effects of Akt inhibition on T cell gene induction. Global microarray analysis was used to characterize T helper cell transcript...
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Gespeichert in:

Autor*in:	Jing Cheng [verfasserIn] Lawrence P Kane [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2013

Schlagwörter:	Genetics of the Immune System Leukocyte Activation Leukocyte Signaling & Gene Expression

Übergeordnetes Werk:	In: F1000Research - F1000 Research Ltd, 2013, 2(2013)
Übergeordnetes Werk:	volume:2 ; year:2013

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.12688/f1000research.2-109.v1

Katalog-ID:	DOAJ009856382

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author	Jing Cheng
spellingShingle	Jing Cheng misc Genetics of the Immune System misc Leukocyte Activation misc Leukocyte Signaling & Gene Expression misc Medicine misc R misc Science misc Q Global identification of genes and pathways regulated by Akt during activation of T helper cells [v1; ref status: indexed, http://f1000r.es/107]
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topic_title	Global identification of genes and pathways regulated by Akt during activation of T helper cells [v1; ref status: indexed, http://f1000r.es/107] Genetics of the Immune System Leukocyte Activation Leukocyte Signaling & Gene Expression
topic	misc Genetics of the Immune System misc Leukocyte Activation misc Leukocyte Signaling & Gene Expression misc Medicine misc R misc Science misc Q
topic_unstemmed	misc Genetics of the Immune System misc Leukocyte Activation misc Leukocyte Signaling & Gene Expression misc Medicine misc R misc Science misc Q
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title	Global identification of genes and pathways regulated by Akt during activation of T helper cells [v1; ref status: indexed, http://f1000r.es/107]
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title_full	Global identification of genes and pathways regulated by Akt during activation of T helper cells [v1; ref status: indexed, http://f1000r.es/107]
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title_sort	global identification of genes and pathways regulated by akt during activation of t helper cells [v1; ref status: indexed, http://f1000r.es/107]
title_auth	Global identification of genes and pathways regulated by Akt during activation of T helper cells [v1; ref status: indexed, http://f1000r.es/107]
abstract	We previously demonstrated that Akt differentially modulated a subset of NF-kB target genes during T cell activation. In the current study, we further explored the broader effects of Akt inhibition on T cell gene induction. Global microarray analysis was used to characterize T helper cell transcriptional responses following antigen receptor stimulation in the absence or presence of Akti1/2 (an allosteric inhibitor which targets Akt1 and Akt2), to identify novel targets dependent upon Akt and obtain a more comprehensive view of Akt-sensitive genes in T helper cells. Pathway analysis of microarray data from a CD4+ T cell line revealed effects on gene networks involving ribosomal and T cell receptor signaling pathways associated with Akti1/2 treatment. Using real-time PCR analysis, we validated differential regulation of several genes in these pathways, including Ier3, Il13, Klf6, Egr1, Ccl1 and Ccl4, among others. Additionally, transcription factor target gene (TFactS) analysis revealed that NF-kB and Myc were the most significantly enriched transcription factors among Akt-dependent genes after T cell receptor and CD28 stimulation. Akt activation elicited increases in the enrichment of NF-kB- and Myc-targeted genes. The present study has identified a diverse set of genes, and possible mechanisms for their regulation, that are dependent on Akt during T cell activation.
abstractGer	We previously demonstrated that Akt differentially modulated a subset of NF-kB target genes during T cell activation. In the current study, we further explored the broader effects of Akt inhibition on T cell gene induction. Global microarray analysis was used to characterize T helper cell transcriptional responses following antigen receptor stimulation in the absence or presence of Akti1/2 (an allosteric inhibitor which targets Akt1 and Akt2), to identify novel targets dependent upon Akt and obtain a more comprehensive view of Akt-sensitive genes in T helper cells. Pathway analysis of microarray data from a CD4+ T cell line revealed effects on gene networks involving ribosomal and T cell receptor signaling pathways associated with Akti1/2 treatment. Using real-time PCR analysis, we validated differential regulation of several genes in these pathways, including Ier3, Il13, Klf6, Egr1, Ccl1 and Ccl4, among others. Additionally, transcription factor target gene (TFactS) analysis revealed that NF-kB and Myc were the most significantly enriched transcription factors among Akt-dependent genes after T cell receptor and CD28 stimulation. Akt activation elicited increases in the enrichment of NF-kB- and Myc-targeted genes. The present study has identified a diverse set of genes, and possible mechanisms for their regulation, that are dependent on Akt during T cell activation.
abstract_unstemmed	We previously demonstrated that Akt differentially modulated a subset of NF-kB target genes during T cell activation. In the current study, we further explored the broader effects of Akt inhibition on T cell gene induction. Global microarray analysis was used to characterize T helper cell transcriptional responses following antigen receptor stimulation in the absence or presence of Akti1/2 (an allosteric inhibitor which targets Akt1 and Akt2), to identify novel targets dependent upon Akt and obtain a more comprehensive view of Akt-sensitive genes in T helper cells. Pathway analysis of microarray data from a CD4+ T cell line revealed effects on gene networks involving ribosomal and T cell receptor signaling pathways associated with Akti1/2 treatment. Using real-time PCR analysis, we validated differential regulation of several genes in these pathways, including Ier3, Il13, Klf6, Egr1, Ccl1 and Ccl4, among others. Additionally, transcription factor target gene (TFactS) analysis revealed that NF-kB and Myc were the most significantly enriched transcription factors among Akt-dependent genes after T cell receptor and CD28 stimulation. Akt activation elicited increases in the enrichment of NF-kB- and Myc-targeted genes. The present study has identified a diverse set of genes, and possible mechanisms for their regulation, that are dependent on Akt during T cell activation.
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title_short	Global identification of genes and pathways regulated by Akt during activation of T helper cells [v1; ref status: indexed, http://f1000r.es/107]
url	https://doi.org/10.12688/f1000research.2-109.v1 https://doaj.org/article/4f142b0cf99b4eac9ff2e97f8d3509a2 http://f1000research.com/articles/2-109/v1 https://doaj.org/toc/2046-1402
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