A neuraminidase activity-based microneutralization assay for evaluating antibody responses to influenza H5 and H7 vaccines.

Outbreaks of the highly pathogenic avian influenza H5N1 and H7N9 viruses have spurred an unprecedented research effort to develop antivirals and vaccines against influenza. Standardized methods for vaccine evaluation are critical for facilitating vaccine development. Compared with hemagglutination i...
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Autor*in:	Hui Zhao [verfasserIn] Kangwei Xu [verfasserIn] Zheng Jiang [verfasserIn] Ming Shao [verfasserIn] Shuzhen Liu [verfasserIn] Xuguang Li [verfasserIn] Junzhi Wang [verfasserIn] Changgui Li [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Übergeordnetes Werk:	In: PLoS ONE - Public Library of Science (PLoS), 2007, 13(2018), 11, p e0207431
Übergeordnetes Werk:	volume:13 ; year:2018 ; number:11, p e0207431

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1371/journal.pone.0207431

Katalog-ID:	DOAJ010380388

Internformat


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245	1	2	\|a A neuraminidase activity-based microneutralization assay for evaluating antibody responses to influenza H5 and H7 vaccines.
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520			\|a Outbreaks of the highly pathogenic avian influenza H5N1 and H7N9 viruses have spurred an unprecedented research effort to develop antivirals and vaccines against influenza. Standardized methods for vaccine evaluation are critical for facilitating vaccine development. Compared with hemagglutination inhibition assays, mounting evidence suggest that microneutralization tests (MNTs) is a better choice for the evaluation of candidate pandemic influenza vaccines because they measure neutralizing antibody activity in cell cultures and are more sensitive in detecting H5 and H7. Here, we report a MNT measuring neuraminidase activity as the read-out (NA-MNT) for quantitative analysis of neutralizing antibodies against avian influenza viruses. Compared to the conventional microneutralization assay (ELISA-MNT), the NA-MNT is faster with lower intra- and inter-assay variations, while no difference in geometric mean titers was found between these two assays for the evaluation of H5N1 and H7N9 vaccines. These results suggest that NA-MNT is a reliable and high throughput method which could facilitate the development of candidate pandemic influenza vaccine.
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700	0		\|a Ming Shao \|e verfasserin \|4 aut
700	0		\|a Shuzhen Liu \|e verfasserin \|4 aut
700	0		\|a Xuguang Li \|e verfasserin \|4 aut
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700	0		\|a Changgui Li \|e verfasserin \|4 aut
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912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
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912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
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912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
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912			\|a GBV_ILN_293
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912			\|a GBV_ILN_702
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912			\|a GBV_ILN_2011
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912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
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912			\|a GBV_ILN_2038
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912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 13 \|j 2018 \|e 11, p e0207431

Indexfelder

author_variant	h z hz k x kx z j zj m s ms s l sl x l xl j w jw c l cl
matchkey_str	article:19326203:2018----::nuaiiaeciiyaemcoetaiainsafrvlaignioyepn
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publishDate	2018
allfields	10.1371/journal.pone.0207431 doi (DE-627)DOAJ010380388 (DE-599)DOAJdf522fcd31e94dd48365b98861faffc6 DE-627 ger DE-627 rakwb eng Hui Zhao verfasserin aut A neuraminidase activity-based microneutralization assay for evaluating antibody responses to influenza H5 and H7 vaccines. 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Outbreaks of the highly pathogenic avian influenza H5N1 and H7N9 viruses have spurred an unprecedented research effort to develop antivirals and vaccines against influenza. Standardized methods for vaccine evaluation are critical for facilitating vaccine development. Compared with hemagglutination inhibition assays, mounting evidence suggest that microneutralization tests (MNTs) is a better choice for the evaluation of candidate pandemic influenza vaccines because they measure neutralizing antibody activity in cell cultures and are more sensitive in detecting H5 and H7. Here, we report a MNT measuring neuraminidase activity as the read-out (NA-MNT) for quantitative analysis of neutralizing antibodies against avian influenza viruses. Compared to the conventional microneutralization assay (ELISA-MNT), the NA-MNT is faster with lower intra- and inter-assay variations, while no difference in geometric mean titers was found between these two assays for the evaluation of H5N1 and H7N9 vaccines. These results suggest that NA-MNT is a reliable and high throughput method which could facilitate the development of candidate pandemic influenza vaccine. Medicine R Science Q Kangwei Xu verfasserin aut Zheng Jiang verfasserin aut Ming Shao verfasserin aut Shuzhen Liu verfasserin aut Xuguang Li verfasserin aut Junzhi Wang verfasserin aut Changgui Li verfasserin aut In PLoS ONE Public Library of Science (PLoS), 2007 13(2018), 11, p e0207431 (DE-627)523574592 (DE-600)2267670-3 19326203 nnns volume:13 year:2018 number:11, p e0207431 https://doi.org/10.1371/journal.pone.0207431 kostenfrei https://doaj.org/article/df522fcd31e94dd48365b98861faffc6 kostenfrei http://europepmc.org/articles/PMC6237356?pdf=render kostenfrei https://doaj.org/toc/1932-6203 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_34 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_235 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2018 11, p e0207431
spelling	10.1371/journal.pone.0207431 doi (DE-627)DOAJ010380388 (DE-599)DOAJdf522fcd31e94dd48365b98861faffc6 DE-627 ger DE-627 rakwb eng Hui Zhao verfasserin aut A neuraminidase activity-based microneutralization assay for evaluating antibody responses to influenza H5 and H7 vaccines. 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Outbreaks of the highly pathogenic avian influenza H5N1 and H7N9 viruses have spurred an unprecedented research effort to develop antivirals and vaccines against influenza. Standardized methods for vaccine evaluation are critical for facilitating vaccine development. Compared with hemagglutination inhibition assays, mounting evidence suggest that microneutralization tests (MNTs) is a better choice for the evaluation of candidate pandemic influenza vaccines because they measure neutralizing antibody activity in cell cultures and are more sensitive in detecting H5 and H7. Here, we report a MNT measuring neuraminidase activity as the read-out (NA-MNT) for quantitative analysis of neutralizing antibodies against avian influenza viruses. Compared to the conventional microneutralization assay (ELISA-MNT), the NA-MNT is faster with lower intra- and inter-assay variations, while no difference in geometric mean titers was found between these two assays for the evaluation of H5N1 and H7N9 vaccines. These results suggest that NA-MNT is a reliable and high throughput method which could facilitate the development of candidate pandemic influenza vaccine. Medicine R Science Q Kangwei Xu verfasserin aut Zheng Jiang verfasserin aut Ming Shao verfasserin aut Shuzhen Liu verfasserin aut Xuguang Li verfasserin aut Junzhi Wang verfasserin aut Changgui Li verfasserin aut In PLoS ONE Public Library of Science (PLoS), 2007 13(2018), 11, p e0207431 (DE-627)523574592 (DE-600)2267670-3 19326203 nnns volume:13 year:2018 number:11, p e0207431 https://doi.org/10.1371/journal.pone.0207431 kostenfrei https://doaj.org/article/df522fcd31e94dd48365b98861faffc6 kostenfrei http://europepmc.org/articles/PMC6237356?pdf=render kostenfrei https://doaj.org/toc/1932-6203 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_34 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_235 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2018 11, p e0207431
allfields_unstemmed	10.1371/journal.pone.0207431 doi (DE-627)DOAJ010380388 (DE-599)DOAJdf522fcd31e94dd48365b98861faffc6 DE-627 ger DE-627 rakwb eng Hui Zhao verfasserin aut A neuraminidase activity-based microneutralization assay for evaluating antibody responses to influenza H5 and H7 vaccines. 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Outbreaks of the highly pathogenic avian influenza H5N1 and H7N9 viruses have spurred an unprecedented research effort to develop antivirals and vaccines against influenza. Standardized methods for vaccine evaluation are critical for facilitating vaccine development. Compared with hemagglutination inhibition assays, mounting evidence suggest that microneutralization tests (MNTs) is a better choice for the evaluation of candidate pandemic influenza vaccines because they measure neutralizing antibody activity in cell cultures and are more sensitive in detecting H5 and H7. Here, we report a MNT measuring neuraminidase activity as the read-out (NA-MNT) for quantitative analysis of neutralizing antibodies against avian influenza viruses. Compared to the conventional microneutralization assay (ELISA-MNT), the NA-MNT is faster with lower intra- and inter-assay variations, while no difference in geometric mean titers was found between these two assays for the evaluation of H5N1 and H7N9 vaccines. These results suggest that NA-MNT is a reliable and high throughput method which could facilitate the development of candidate pandemic influenza vaccine. Medicine R Science Q Kangwei Xu verfasserin aut Zheng Jiang verfasserin aut Ming Shao verfasserin aut Shuzhen Liu verfasserin aut Xuguang Li verfasserin aut Junzhi Wang verfasserin aut Changgui Li verfasserin aut In PLoS ONE Public Library of Science (PLoS), 2007 13(2018), 11, p e0207431 (DE-627)523574592 (DE-600)2267670-3 19326203 nnns volume:13 year:2018 number:11, p e0207431 https://doi.org/10.1371/journal.pone.0207431 kostenfrei https://doaj.org/article/df522fcd31e94dd48365b98861faffc6 kostenfrei http://europepmc.org/articles/PMC6237356?pdf=render kostenfrei https://doaj.org/toc/1932-6203 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_34 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_235 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2018 11, p e0207431
allfieldsGer	10.1371/journal.pone.0207431 doi (DE-627)DOAJ010380388 (DE-599)DOAJdf522fcd31e94dd48365b98861faffc6 DE-627 ger DE-627 rakwb eng Hui Zhao verfasserin aut A neuraminidase activity-based microneutralization assay for evaluating antibody responses to influenza H5 and H7 vaccines. 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Outbreaks of the highly pathogenic avian influenza H5N1 and H7N9 viruses have spurred an unprecedented research effort to develop antivirals and vaccines against influenza. Standardized methods for vaccine evaluation are critical for facilitating vaccine development. Compared with hemagglutination inhibition assays, mounting evidence suggest that microneutralization tests (MNTs) is a better choice for the evaluation of candidate pandemic influenza vaccines because they measure neutralizing antibody activity in cell cultures and are more sensitive in detecting H5 and H7. Here, we report a MNT measuring neuraminidase activity as the read-out (NA-MNT) for quantitative analysis of neutralizing antibodies against avian influenza viruses. Compared to the conventional microneutralization assay (ELISA-MNT), the NA-MNT is faster with lower intra- and inter-assay variations, while no difference in geometric mean titers was found between these two assays for the evaluation of H5N1 and H7N9 vaccines. These results suggest that NA-MNT is a reliable and high throughput method which could facilitate the development of candidate pandemic influenza vaccine. Medicine R Science Q Kangwei Xu verfasserin aut Zheng Jiang verfasserin aut Ming Shao verfasserin aut Shuzhen Liu verfasserin aut Xuguang Li verfasserin aut Junzhi Wang verfasserin aut Changgui Li verfasserin aut In PLoS ONE Public Library of Science (PLoS), 2007 13(2018), 11, p e0207431 (DE-627)523574592 (DE-600)2267670-3 19326203 nnns volume:13 year:2018 number:11, p e0207431 https://doi.org/10.1371/journal.pone.0207431 kostenfrei https://doaj.org/article/df522fcd31e94dd48365b98861faffc6 kostenfrei http://europepmc.org/articles/PMC6237356?pdf=render kostenfrei https://doaj.org/toc/1932-6203 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_34 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_235 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2018 11, p e0207431
allfieldsSound	10.1371/journal.pone.0207431 doi (DE-627)DOAJ010380388 (DE-599)DOAJdf522fcd31e94dd48365b98861faffc6 DE-627 ger DE-627 rakwb eng Hui Zhao verfasserin aut A neuraminidase activity-based microneutralization assay for evaluating antibody responses to influenza H5 and H7 vaccines. 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Outbreaks of the highly pathogenic avian influenza H5N1 and H7N9 viruses have spurred an unprecedented research effort to develop antivirals and vaccines against influenza. Standardized methods for vaccine evaluation are critical for facilitating vaccine development. Compared with hemagglutination inhibition assays, mounting evidence suggest that microneutralization tests (MNTs) is a better choice for the evaluation of candidate pandemic influenza vaccines because they measure neutralizing antibody activity in cell cultures and are more sensitive in detecting H5 and H7. Here, we report a MNT measuring neuraminidase activity as the read-out (NA-MNT) for quantitative analysis of neutralizing antibodies against avian influenza viruses. Compared to the conventional microneutralization assay (ELISA-MNT), the NA-MNT is faster with lower intra- and inter-assay variations, while no difference in geometric mean titers was found between these two assays for the evaluation of H5N1 and H7N9 vaccines. These results suggest that NA-MNT is a reliable and high throughput method which could facilitate the development of candidate pandemic influenza vaccine. Medicine R Science Q Kangwei Xu verfasserin aut Zheng Jiang verfasserin aut Ming Shao verfasserin aut Shuzhen Liu verfasserin aut Xuguang Li verfasserin aut Junzhi Wang verfasserin aut Changgui Li verfasserin aut In PLoS ONE Public Library of Science (PLoS), 2007 13(2018), 11, p e0207431 (DE-627)523574592 (DE-600)2267670-3 19326203 nnns volume:13 year:2018 number:11, p e0207431 https://doi.org/10.1371/journal.pone.0207431 kostenfrei https://doaj.org/article/df522fcd31e94dd48365b98861faffc6 kostenfrei http://europepmc.org/articles/PMC6237356?pdf=render kostenfrei https://doaj.org/toc/1932-6203 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_34 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_235 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2018 11, p e0207431
language	English
source	In PLoS ONE 13(2018), 11, p e0207431 volume:13 year:2018 number:11, p e0207431
sourceStr	In PLoS ONE 13(2018), 11, p e0207431 volume:13 year:2018 number:11, p e0207431
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Medicine R Science Q
isfreeaccess_bool	true
container_title	PLoS ONE
authorswithroles_txt_mv	Hui Zhao @@aut@@ Kangwei Xu @@aut@@ Zheng Jiang @@aut@@ Ming Shao @@aut@@ Shuzhen Liu @@aut@@ Xuguang Li @@aut@@ Junzhi Wang @@aut@@ Changgui Li @@aut@@
publishDateDaySort_date	2018-01-01T00:00:00Z
hierarchy_top_id	523574592
id	DOAJ010380388
language_de	englisch
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author	Hui Zhao
spellingShingle	Hui Zhao misc Medicine misc R misc Science misc Q A neuraminidase activity-based microneutralization assay for evaluating antibody responses to influenza H5 and H7 vaccines.
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topic_title	A neuraminidase activity-based microneutralization assay for evaluating antibody responses to influenza H5 and H7 vaccines
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title	A neuraminidase activity-based microneutralization assay for evaluating antibody responses to influenza H5 and H7 vaccines.
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title_full	A neuraminidase activity-based microneutralization assay for evaluating antibody responses to influenza H5 and H7 vaccines
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author_browse	Hui Zhao Kangwei Xu Zheng Jiang Ming Shao Shuzhen Liu Xuguang Li Junzhi Wang Changgui Li
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author-letter	Hui Zhao
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title_sort	neuraminidase activity-based microneutralization assay for evaluating antibody responses to influenza h5 and h7 vaccines
title_auth	A neuraminidase activity-based microneutralization assay for evaluating antibody responses to influenza H5 and H7 vaccines.
abstract	Outbreaks of the highly pathogenic avian influenza H5N1 and H7N9 viruses have spurred an unprecedented research effort to develop antivirals and vaccines against influenza. Standardized methods for vaccine evaluation are critical for facilitating vaccine development. Compared with hemagglutination inhibition assays, mounting evidence suggest that microneutralization tests (MNTs) is a better choice for the evaluation of candidate pandemic influenza vaccines because they measure neutralizing antibody activity in cell cultures and are more sensitive in detecting H5 and H7. Here, we report a MNT measuring neuraminidase activity as the read-out (NA-MNT) for quantitative analysis of neutralizing antibodies against avian influenza viruses. Compared to the conventional microneutralization assay (ELISA-MNT), the NA-MNT is faster with lower intra- and inter-assay variations, while no difference in geometric mean titers was found between these two assays for the evaluation of H5N1 and H7N9 vaccines. These results suggest that NA-MNT is a reliable and high throughput method which could facilitate the development of candidate pandemic influenza vaccine.
abstractGer	Outbreaks of the highly pathogenic avian influenza H5N1 and H7N9 viruses have spurred an unprecedented research effort to develop antivirals and vaccines against influenza. Standardized methods for vaccine evaluation are critical for facilitating vaccine development. Compared with hemagglutination inhibition assays, mounting evidence suggest that microneutralization tests (MNTs) is a better choice for the evaluation of candidate pandemic influenza vaccines because they measure neutralizing antibody activity in cell cultures and are more sensitive in detecting H5 and H7. Here, we report a MNT measuring neuraminidase activity as the read-out (NA-MNT) for quantitative analysis of neutralizing antibodies against avian influenza viruses. Compared to the conventional microneutralization assay (ELISA-MNT), the NA-MNT is faster with lower intra- and inter-assay variations, while no difference in geometric mean titers was found between these two assays for the evaluation of H5N1 and H7N9 vaccines. These results suggest that NA-MNT is a reliable and high throughput method which could facilitate the development of candidate pandemic influenza vaccine.
abstract_unstemmed	Outbreaks of the highly pathogenic avian influenza H5N1 and H7N9 viruses have spurred an unprecedented research effort to develop antivirals and vaccines against influenza. Standardized methods for vaccine evaluation are critical for facilitating vaccine development. Compared with hemagglutination inhibition assays, mounting evidence suggest that microneutralization tests (MNTs) is a better choice for the evaluation of candidate pandemic influenza vaccines because they measure neutralizing antibody activity in cell cultures and are more sensitive in detecting H5 and H7. Here, we report a MNT measuring neuraminidase activity as the read-out (NA-MNT) for quantitative analysis of neutralizing antibodies against avian influenza viruses. Compared to the conventional microneutralization assay (ELISA-MNT), the NA-MNT is faster with lower intra- and inter-assay variations, while no difference in geometric mean titers was found between these two assays for the evaluation of H5N1 and H7N9 vaccines. These results suggest that NA-MNT is a reliable and high throughput method which could facilitate the development of candidate pandemic influenza vaccine.
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title_short	A neuraminidase activity-based microneutralization assay for evaluating antibody responses to influenza H5 and H7 vaccines.
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up_date	2024-07-03T14:30:34.001Z
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A neuraminidase activity-based microneutralization assay for evaluating antibody responses to influenza H5 and H7 vaccines.

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