Multiple ESBL-producing Escherichia coli sequence types carrying quinolone and aminoglycoside resistance genes circulating in companion and domestic farm animals in Mwanza, Tanzania, harbor commonly occurring plasmids

The increased presence of extended-spectrum beta-lactamase (ESBL)-producing bacteria in humans, animals and their surrounding environments is of global concern. Currently there is limited information on ESBL presence in rural farming communities worldwide. We performed a cross-sectional study in Mwa...
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Autor*in:	Jeremiah eSeni [verfasserIn] Linda eFalgenhauer [verfasserIn] Nabina eSimeo [verfasserIn] Mariam M Mirambo [verfasserIn] Can eImirzalioglu [verfasserIn] Mecky eMatee [verfasserIn] Mark eRweyemamu [verfasserIn] Trinad eChakraborty [verfasserIn] Stephen E Mshana [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	Tanzania ESBL companion animals domestic farm animals Mwanza Farming communities

Übergeordnetes Werk:	In: Frontiers in Microbiology - Frontiers Media S.A., 2011, 7(2016)
Übergeordnetes Werk:	volume:7 ; year:2016

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fmicb.2016.00142

Katalog-ID:	DOAJ010831711

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allfields	10.3389/fmicb.2016.00142 doi (DE-627)DOAJ010831711 (DE-599)DOAJ8216e48bf4fb455fbc893bd861037080 DE-627 ger DE-627 rakwb eng QR1-502 Jeremiah eSeni verfasserin aut Multiple ESBL-producing Escherichia coli sequence types carrying quinolone and aminoglycoside resistance genes circulating in companion and domestic farm animals in Mwanza, Tanzania, harbor commonly occurring plasmids 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The increased presence of extended-spectrum beta-lactamase (ESBL)-producing bacteria in humans, animals and their surrounding environments is of global concern. Currently there is limited information on ESBL presence in rural farming communities worldwide. We performed a cross-sectional study in Mwanza, Tanzania, involving 600 companion and domestic farm animals between August/September 2014. Rectal swab/cloaca specimens were processed to identify ESBL-producing Enterobacteriaceae. We detected 130 (21.7%) animals carrying ESBL-producing bacteria, the highest carriage being among dogs and pigs [39.2% (51/130) and 33.1% (43/130), respectively]. The majority of isolates were Escherichia coli [93.3% (125/134)] and exotic breed type [OR (95%CI) = 2.372 (1.460 - 3.854), p-value <0.001] was found to be a predictor of ESBL carriage among animals. Whole-genome sequences of 25 ESBL-producing E. coli were analyzed for phylogenetic relationships using multi-locus sequence typing (MLST) and core genome comparisons. Fourteen different sequence types were detected of which ST617 (7/25), ST2852 (3/25), ST1303 (3/25) were the most abundant. All isolates harbored the blaCTX-M-15 allele, 22/25 carried strA and strB, 12/25 aac(6’)Ib-cr and 11/25 qnrS1. Antibiotic resistance was associated with IncF, IncY, as well as non-typable plasmids. Eleven isolates carried pPGRT46-related plasmids, previously reported from isolates in Nigeria. Five isolates had plasmids exhibiting 85-99% homology to pCA28, previously detected in isolates from the United States. Our findings indicate a pan-species distribution of ESBL-producing E. coli clonal groups in farming communities and provide evidence for plasmids harboring antibiotic resistances of regional and international impact. Tanzania ESBL companion animals domestic farm animals Mwanza Farming communities Microbiology Linda eFalgenhauer verfasserin aut Linda eFalgenhauer verfasserin aut Nabina eSimeo verfasserin aut Mariam M Mirambo verfasserin aut Can eImirzalioglu verfasserin aut Can eImirzalioglu verfasserin aut Mecky eMatee verfasserin aut Mark eRweyemamu verfasserin aut Trinad eChakraborty verfasserin aut Trinad eChakraborty verfasserin aut Stephen E Mshana verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 7(2016) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:7 year:2016 https://doi.org/10.3389/fmicb.2016.00142 kostenfrei https://doaj.org/article/8216e48bf4fb455fbc893bd861037080 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00142/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2016
spelling	10.3389/fmicb.2016.00142 doi (DE-627)DOAJ010831711 (DE-599)DOAJ8216e48bf4fb455fbc893bd861037080 DE-627 ger DE-627 rakwb eng QR1-502 Jeremiah eSeni verfasserin aut Multiple ESBL-producing Escherichia coli sequence types carrying quinolone and aminoglycoside resistance genes circulating in companion and domestic farm animals in Mwanza, Tanzania, harbor commonly occurring plasmids 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The increased presence of extended-spectrum beta-lactamase (ESBL)-producing bacteria in humans, animals and their surrounding environments is of global concern. Currently there is limited information on ESBL presence in rural farming communities worldwide. We performed a cross-sectional study in Mwanza, Tanzania, involving 600 companion and domestic farm animals between August/September 2014. Rectal swab/cloaca specimens were processed to identify ESBL-producing Enterobacteriaceae. We detected 130 (21.7%) animals carrying ESBL-producing bacteria, the highest carriage being among dogs and pigs [39.2% (51/130) and 33.1% (43/130), respectively]. The majority of isolates were Escherichia coli [93.3% (125/134)] and exotic breed type [OR (95%CI) = 2.372 (1.460 - 3.854), p-value <0.001] was found to be a predictor of ESBL carriage among animals. Whole-genome sequences of 25 ESBL-producing E. coli were analyzed for phylogenetic relationships using multi-locus sequence typing (MLST) and core genome comparisons. Fourteen different sequence types were detected of which ST617 (7/25), ST2852 (3/25), ST1303 (3/25) were the most abundant. All isolates harbored the blaCTX-M-15 allele, 22/25 carried strA and strB, 12/25 aac(6’)Ib-cr and 11/25 qnrS1. Antibiotic resistance was associated with IncF, IncY, as well as non-typable plasmids. Eleven isolates carried pPGRT46-related plasmids, previously reported from isolates in Nigeria. Five isolates had plasmids exhibiting 85-99% homology to pCA28, previously detected in isolates from the United States. Our findings indicate a pan-species distribution of ESBL-producing E. coli clonal groups in farming communities and provide evidence for plasmids harboring antibiotic resistances of regional and international impact. Tanzania ESBL companion animals domestic farm animals Mwanza Farming communities Microbiology Linda eFalgenhauer verfasserin aut Linda eFalgenhauer verfasserin aut Nabina eSimeo verfasserin aut Mariam M Mirambo verfasserin aut Can eImirzalioglu verfasserin aut Can eImirzalioglu verfasserin aut Mecky eMatee verfasserin aut Mark eRweyemamu verfasserin aut Trinad eChakraborty verfasserin aut Trinad eChakraborty verfasserin aut Stephen E Mshana verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 7(2016) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:7 year:2016 https://doi.org/10.3389/fmicb.2016.00142 kostenfrei https://doaj.org/article/8216e48bf4fb455fbc893bd861037080 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00142/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2016
allfields_unstemmed	10.3389/fmicb.2016.00142 doi (DE-627)DOAJ010831711 (DE-599)DOAJ8216e48bf4fb455fbc893bd861037080 DE-627 ger DE-627 rakwb eng QR1-502 Jeremiah eSeni verfasserin aut Multiple ESBL-producing Escherichia coli sequence types carrying quinolone and aminoglycoside resistance genes circulating in companion and domestic farm animals in Mwanza, Tanzania, harbor commonly occurring plasmids 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The increased presence of extended-spectrum beta-lactamase (ESBL)-producing bacteria in humans, animals and their surrounding environments is of global concern. Currently there is limited information on ESBL presence in rural farming communities worldwide. We performed a cross-sectional study in Mwanza, Tanzania, involving 600 companion and domestic farm animals between August/September 2014. Rectal swab/cloaca specimens were processed to identify ESBL-producing Enterobacteriaceae. We detected 130 (21.7%) animals carrying ESBL-producing bacteria, the highest carriage being among dogs and pigs [39.2% (51/130) and 33.1% (43/130), respectively]. The majority of isolates were Escherichia coli [93.3% (125/134)] and exotic breed type [OR (95%CI) = 2.372 (1.460 - 3.854), p-value <0.001] was found to be a predictor of ESBL carriage among animals. Whole-genome sequences of 25 ESBL-producing E. coli were analyzed for phylogenetic relationships using multi-locus sequence typing (MLST) and core genome comparisons. Fourteen different sequence types were detected of which ST617 (7/25), ST2852 (3/25), ST1303 (3/25) were the most abundant. All isolates harbored the blaCTX-M-15 allele, 22/25 carried strA and strB, 12/25 aac(6’)Ib-cr and 11/25 qnrS1. Antibiotic resistance was associated with IncF, IncY, as well as non-typable plasmids. Eleven isolates carried pPGRT46-related plasmids, previously reported from isolates in Nigeria. Five isolates had plasmids exhibiting 85-99% homology to pCA28, previously detected in isolates from the United States. Our findings indicate a pan-species distribution of ESBL-producing E. coli clonal groups in farming communities and provide evidence for plasmids harboring antibiotic resistances of regional and international impact. Tanzania ESBL companion animals domestic farm animals Mwanza Farming communities Microbiology Linda eFalgenhauer verfasserin aut Linda eFalgenhauer verfasserin aut Nabina eSimeo verfasserin aut Mariam M Mirambo verfasserin aut Can eImirzalioglu verfasserin aut Can eImirzalioglu verfasserin aut Mecky eMatee verfasserin aut Mark eRweyemamu verfasserin aut Trinad eChakraborty verfasserin aut Trinad eChakraborty verfasserin aut Stephen E Mshana verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 7(2016) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:7 year:2016 https://doi.org/10.3389/fmicb.2016.00142 kostenfrei https://doaj.org/article/8216e48bf4fb455fbc893bd861037080 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00142/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2016
allfieldsGer	10.3389/fmicb.2016.00142 doi (DE-627)DOAJ010831711 (DE-599)DOAJ8216e48bf4fb455fbc893bd861037080 DE-627 ger DE-627 rakwb eng QR1-502 Jeremiah eSeni verfasserin aut Multiple ESBL-producing Escherichia coli sequence types carrying quinolone and aminoglycoside resistance genes circulating in companion and domestic farm animals in Mwanza, Tanzania, harbor commonly occurring plasmids 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The increased presence of extended-spectrum beta-lactamase (ESBL)-producing bacteria in humans, animals and their surrounding environments is of global concern. Currently there is limited information on ESBL presence in rural farming communities worldwide. We performed a cross-sectional study in Mwanza, Tanzania, involving 600 companion and domestic farm animals between August/September 2014. Rectal swab/cloaca specimens were processed to identify ESBL-producing Enterobacteriaceae. We detected 130 (21.7%) animals carrying ESBL-producing bacteria, the highest carriage being among dogs and pigs [39.2% (51/130) and 33.1% (43/130), respectively]. The majority of isolates were Escherichia coli [93.3% (125/134)] and exotic breed type [OR (95%CI) = 2.372 (1.460 - 3.854), p-value <0.001] was found to be a predictor of ESBL carriage among animals. Whole-genome sequences of 25 ESBL-producing E. coli were analyzed for phylogenetic relationships using multi-locus sequence typing (MLST) and core genome comparisons. Fourteen different sequence types were detected of which ST617 (7/25), ST2852 (3/25), ST1303 (3/25) were the most abundant. All isolates harbored the blaCTX-M-15 allele, 22/25 carried strA and strB, 12/25 aac(6’)Ib-cr and 11/25 qnrS1. Antibiotic resistance was associated with IncF, IncY, as well as non-typable plasmids. Eleven isolates carried pPGRT46-related plasmids, previously reported from isolates in Nigeria. Five isolates had plasmids exhibiting 85-99% homology to pCA28, previously detected in isolates from the United States. Our findings indicate a pan-species distribution of ESBL-producing E. coli clonal groups in farming communities and provide evidence for plasmids harboring antibiotic resistances of regional and international impact. Tanzania ESBL companion animals domestic farm animals Mwanza Farming communities Microbiology Linda eFalgenhauer verfasserin aut Linda eFalgenhauer verfasserin aut Nabina eSimeo verfasserin aut Mariam M Mirambo verfasserin aut Can eImirzalioglu verfasserin aut Can eImirzalioglu verfasserin aut Mecky eMatee verfasserin aut Mark eRweyemamu verfasserin aut Trinad eChakraborty verfasserin aut Trinad eChakraborty verfasserin aut Stephen E Mshana verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 7(2016) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:7 year:2016 https://doi.org/10.3389/fmicb.2016.00142 kostenfrei https://doaj.org/article/8216e48bf4fb455fbc893bd861037080 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00142/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2016
allfieldsSound	10.3389/fmicb.2016.00142 doi (DE-627)DOAJ010831711 (DE-599)DOAJ8216e48bf4fb455fbc893bd861037080 DE-627 ger DE-627 rakwb eng QR1-502 Jeremiah eSeni verfasserin aut Multiple ESBL-producing Escherichia coli sequence types carrying quinolone and aminoglycoside resistance genes circulating in companion and domestic farm animals in Mwanza, Tanzania, harbor commonly occurring plasmids 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The increased presence of extended-spectrum beta-lactamase (ESBL)-producing bacteria in humans, animals and their surrounding environments is of global concern. Currently there is limited information on ESBL presence in rural farming communities worldwide. We performed a cross-sectional study in Mwanza, Tanzania, involving 600 companion and domestic farm animals between August/September 2014. Rectal swab/cloaca specimens were processed to identify ESBL-producing Enterobacteriaceae. We detected 130 (21.7%) animals carrying ESBL-producing bacteria, the highest carriage being among dogs and pigs [39.2% (51/130) and 33.1% (43/130), respectively]. The majority of isolates were Escherichia coli [93.3% (125/134)] and exotic breed type [OR (95%CI) = 2.372 (1.460 - 3.854), p-value <0.001] was found to be a predictor of ESBL carriage among animals. Whole-genome sequences of 25 ESBL-producing E. coli were analyzed for phylogenetic relationships using multi-locus sequence typing (MLST) and core genome comparisons. Fourteen different sequence types were detected of which ST617 (7/25), ST2852 (3/25), ST1303 (3/25) were the most abundant. All isolates harbored the blaCTX-M-15 allele, 22/25 carried strA and strB, 12/25 aac(6’)Ib-cr and 11/25 qnrS1. Antibiotic resistance was associated with IncF, IncY, as well as non-typable plasmids. Eleven isolates carried pPGRT46-related plasmids, previously reported from isolates in Nigeria. Five isolates had plasmids exhibiting 85-99% homology to pCA28, previously detected in isolates from the United States. Our findings indicate a pan-species distribution of ESBL-producing E. coli clonal groups in farming communities and provide evidence for plasmids harboring antibiotic resistances of regional and international impact. Tanzania ESBL companion animals domestic farm animals Mwanza Farming communities Microbiology Linda eFalgenhauer verfasserin aut Linda eFalgenhauer verfasserin aut Nabina eSimeo verfasserin aut Mariam M Mirambo verfasserin aut Can eImirzalioglu verfasserin aut Can eImirzalioglu verfasserin aut Mecky eMatee verfasserin aut Mark eRweyemamu verfasserin aut Trinad eChakraborty verfasserin aut Trinad eChakraborty verfasserin aut Stephen E Mshana verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 7(2016) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:7 year:2016 https://doi.org/10.3389/fmicb.2016.00142 kostenfrei https://doaj.org/article/8216e48bf4fb455fbc893bd861037080 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00142/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2016
language	English
source	In Frontiers in Microbiology 7(2016) volume:7 year:2016
sourceStr	In Frontiers in Microbiology 7(2016) volume:7 year:2016
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Tanzania ESBL companion animals domestic farm animals Mwanza Farming communities Microbiology
isfreeaccess_bool	true
container_title	Frontiers in Microbiology
authorswithroles_txt_mv	Jeremiah eSeni @@aut@@ Linda eFalgenhauer @@aut@@ Nabina eSimeo @@aut@@ Mariam M Mirambo @@aut@@ Can eImirzalioglu @@aut@@ Mecky eMatee @@aut@@ Mark eRweyemamu @@aut@@ Trinad eChakraborty @@aut@@ Stephen E Mshana @@aut@@
publishDateDaySort_date	2016-01-01T00:00:00Z
hierarchy_top_id	642889384
id	DOAJ010831711
language_de	englisch
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author	Jeremiah eSeni
spellingShingle	Jeremiah eSeni misc QR1-502 misc Tanzania misc ESBL misc companion animals misc domestic farm animals misc Mwanza misc Farming communities misc Microbiology Multiple ESBL-producing Escherichia coli sequence types carrying quinolone and aminoglycoside resistance genes circulating in companion and domestic farm animals in Mwanza, Tanzania, harbor commonly occurring plasmids
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topic_title	QR1-502 Multiple ESBL-producing Escherichia coli sequence types carrying quinolone and aminoglycoside resistance genes circulating in companion and domestic farm animals in Mwanza, Tanzania, harbor commonly occurring plasmids Tanzania ESBL companion animals domestic farm animals Mwanza Farming communities
topic	misc QR1-502 misc Tanzania misc ESBL misc companion animals misc domestic farm animals misc Mwanza misc Farming communities misc Microbiology
topic_unstemmed	misc QR1-502 misc Tanzania misc ESBL misc companion animals misc domestic farm animals misc Mwanza misc Farming communities misc Microbiology
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title	Multiple ESBL-producing Escherichia coli sequence types carrying quinolone and aminoglycoside resistance genes circulating in companion and domestic farm animals in Mwanza, Tanzania, harbor commonly occurring plasmids
ctrlnum	(DE-627)DOAJ010831711 (DE-599)DOAJ8216e48bf4fb455fbc893bd861037080
title_full	Multiple ESBL-producing Escherichia coli sequence types carrying quinolone and aminoglycoside resistance genes circulating in companion and domestic farm animals in Mwanza, Tanzania, harbor commonly occurring plasmids
author_sort	Jeremiah eSeni
journal	Frontiers in Microbiology
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author_browse	Jeremiah eSeni Linda eFalgenhauer Nabina eSimeo Mariam M Mirambo Can eImirzalioglu Mecky eMatee Mark eRweyemamu Trinad eChakraborty Stephen E Mshana
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title_sort	multiple esbl-producing escherichia coli sequence types carrying quinolone and aminoglycoside resistance genes circulating in companion and domestic farm animals in mwanza, tanzania, harbor commonly occurring plasmids
callnumber	QR1-502
title_auth	Multiple ESBL-producing Escherichia coli sequence types carrying quinolone and aminoglycoside resistance genes circulating in companion and domestic farm animals in Mwanza, Tanzania, harbor commonly occurring plasmids
abstract	The increased presence of extended-spectrum beta-lactamase (ESBL)-producing bacteria in humans, animals and their surrounding environments is of global concern. Currently there is limited information on ESBL presence in rural farming communities worldwide. We performed a cross-sectional study in Mwanza, Tanzania, involving 600 companion and domestic farm animals between August/September 2014. Rectal swab/cloaca specimens were processed to identify ESBL-producing Enterobacteriaceae. We detected 130 (21.7%) animals carrying ESBL-producing bacteria, the highest carriage being among dogs and pigs [39.2% (51/130) and 33.1% (43/130), respectively]. The majority of isolates were Escherichia coli [93.3% (125/134)] and exotic breed type [OR (95%CI) = 2.372 (1.460 - 3.854), p-value <0.001] was found to be a predictor of ESBL carriage among animals. Whole-genome sequences of 25 ESBL-producing E. coli were analyzed for phylogenetic relationships using multi-locus sequence typing (MLST) and core genome comparisons. Fourteen different sequence types were detected of which ST617 (7/25), ST2852 (3/25), ST1303 (3/25) were the most abundant. All isolates harbored the blaCTX-M-15 allele, 22/25 carried strA and strB, 12/25 aac(6’)Ib-cr and 11/25 qnrS1. Antibiotic resistance was associated with IncF, IncY, as well as non-typable plasmids. Eleven isolates carried pPGRT46-related plasmids, previously reported from isolates in Nigeria. Five isolates had plasmids exhibiting 85-99% homology to pCA28, previously detected in isolates from the United States. Our findings indicate a pan-species distribution of ESBL-producing E. coli clonal groups in farming communities and provide evidence for plasmids harboring antibiotic resistances of regional and international impact.
abstractGer	The increased presence of extended-spectrum beta-lactamase (ESBL)-producing bacteria in humans, animals and their surrounding environments is of global concern. Currently there is limited information on ESBL presence in rural farming communities worldwide. We performed a cross-sectional study in Mwanza, Tanzania, involving 600 companion and domestic farm animals between August/September 2014. Rectal swab/cloaca specimens were processed to identify ESBL-producing Enterobacteriaceae. We detected 130 (21.7%) animals carrying ESBL-producing bacteria, the highest carriage being among dogs and pigs [39.2% (51/130) and 33.1% (43/130), respectively]. The majority of isolates were Escherichia coli [93.3% (125/134)] and exotic breed type [OR (95%CI) = 2.372 (1.460 - 3.854), p-value <0.001] was found to be a predictor of ESBL carriage among animals. Whole-genome sequences of 25 ESBL-producing E. coli were analyzed for phylogenetic relationships using multi-locus sequence typing (MLST) and core genome comparisons. Fourteen different sequence types were detected of which ST617 (7/25), ST2852 (3/25), ST1303 (3/25) were the most abundant. All isolates harbored the blaCTX-M-15 allele, 22/25 carried strA and strB, 12/25 aac(6’)Ib-cr and 11/25 qnrS1. Antibiotic resistance was associated with IncF, IncY, as well as non-typable plasmids. Eleven isolates carried pPGRT46-related plasmids, previously reported from isolates in Nigeria. Five isolates had plasmids exhibiting 85-99% homology to pCA28, previously detected in isolates from the United States. Our findings indicate a pan-species distribution of ESBL-producing E. coli clonal groups in farming communities and provide evidence for plasmids harboring antibiotic resistances of regional and international impact.
abstract_unstemmed	The increased presence of extended-spectrum beta-lactamase (ESBL)-producing bacteria in humans, animals and their surrounding environments is of global concern. Currently there is limited information on ESBL presence in rural farming communities worldwide. We performed a cross-sectional study in Mwanza, Tanzania, involving 600 companion and domestic farm animals between August/September 2014. Rectal swab/cloaca specimens were processed to identify ESBL-producing Enterobacteriaceae. We detected 130 (21.7%) animals carrying ESBL-producing bacteria, the highest carriage being among dogs and pigs [39.2% (51/130) and 33.1% (43/130), respectively]. The majority of isolates were Escherichia coli [93.3% (125/134)] and exotic breed type [OR (95%CI) = 2.372 (1.460 - 3.854), p-value <0.001] was found to be a predictor of ESBL carriage among animals. Whole-genome sequences of 25 ESBL-producing E. coli were analyzed for phylogenetic relationships using multi-locus sequence typing (MLST) and core genome comparisons. Fourteen different sequence types were detected of which ST617 (7/25), ST2852 (3/25), ST1303 (3/25) were the most abundant. All isolates harbored the blaCTX-M-15 allele, 22/25 carried strA and strB, 12/25 aac(6’)Ib-cr and 11/25 qnrS1. Antibiotic resistance was associated with IncF, IncY, as well as non-typable plasmids. Eleven isolates carried pPGRT46-related plasmids, previously reported from isolates in Nigeria. Five isolates had plasmids exhibiting 85-99% homology to pCA28, previously detected in isolates from the United States. Our findings indicate a pan-species distribution of ESBL-producing E. coli clonal groups in farming communities and provide evidence for plasmids harboring antibiotic resistances of regional and international impact.
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title_short	Multiple ESBL-producing Escherichia coli sequence types carrying quinolone and aminoglycoside resistance genes circulating in companion and domestic farm animals in Mwanza, Tanzania, harbor commonly occurring plasmids
url	https://doi.org/10.3389/fmicb.2016.00142 https://doaj.org/article/8216e48bf4fb455fbc893bd861037080 http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00142/full https://doaj.org/toc/1664-302X
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Multiple ESBL-producing Escherichia coli sequence types carrying quinolone and aminoglycoside resistance genes circulating in companion and domestic farm animals in Mwanza, Tanzania, harbor commonly occurring plasmids

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