Evaluation of semen parameters of boars (Sus scrofa) experimentally infected with Toxoplasma gondii/ <br< Avaliação dos parâmetros seminais de cachaços (Sus scrofa) experimentalmente infectados com Toxoplasma gondii
Aiming to investigate the influence of T. gondii on semen parameters and spermatozoa morphology, eight boars were inoculated with T. gondii. Experimental groups consisted of: GI (n=3) 1,5 x 104 oocysts of P strain; GII (n=3) 1,0 x 106 tachyzoites of RH strain and GIII (n=2), control noninoculated. E...
Ausführliche Beschreibung
Autor*in: |
Alvimar José da Costa [verfasserIn] Fernanda de Rezende Pinto [verfasserIn] Beatriz Bassora Paim [verfasserIn] Daniela Cristina Di Mauro [verfasserIn] Sérgio Jordão Filho [verfasserIn] Anderson Barbosa de Moura [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch ; Portugiesisch |
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2004 |
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Übergeordnetes Werk: |
In: Semina: Ciências Agrárias - Universidade Estadual de Londrina, 2010, 25(2004), 2, Seite 107-116 |
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Übergeordnetes Werk: |
volume:25 ; year:2004 ; number:2 ; pages:107-116 |
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Link aufrufen |
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DOI / URN: |
10.5433/1679-0359.2004v25n2p107 |
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Katalog-ID: |
DOAJ010842322 |
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520 | |a Aiming to investigate the influence of T. gondii on semen parameters and spermatozoa morphology, eight boars were inoculated with T. gondii. Experimental groups consisted of: GI (n=3) 1,5 x 104 oocysts of P strain; GII (n=3) 1,0 x 106 tachyzoites of RH strain and GIII (n=2), control noninoculated. Evaluations of semen parameters (volume, motility, strength, concentration), study of spermatozoa morphology, serology (RIFI), parasitemia and hemograms were performed. For this purpose, blood and semen collection were carried out on days -2, -1, 1, 3, 5, 7, 9, 11, 14 and weekly until 84 days post-inoculation. Non hematimetrics alterations and clinical signs were observed on animals. Parasitemia was detected in an animal inoculated with oocysts, on the 7th day post inoculation (DPI) and in a two pigs of GII (tachyzoites), on the 3rd and 49th DPI. Serology results revealed the presence of antibody anti-T. gondii on the animals inoculated with oocysts or tachyzoites since 7th DPI, with tittles of 256 and 64, reaching a maximal level of 4096 on days 11 and 9 post inoculation, respectively. The GIII (control) was negative through out all experimental period. The semen parametersevaluated did not present any alteration due to toxoplasmosis. Significative differences (P<0,05), considering spermatozoa pathology, were not observed between inoculated and control groups.<p<<p<Com o objetivo de investigar a influência do Toxoplasma gondii nos parâmetros seminais e na morfologia espermática de suínos, oito reprodutores foram inoculados com T. gondii, sendo constituídos os seguintes grupos experimentais: GI (n=3) 1,5 x 104 oocistos da cepa P, via oral; GII (n=3) 1,0 x 106 taquizoítos da cepa RH, via subcutânea e GIII (n=2), controle. Foram realizadas avaliações de parâmetros espermáticos (volume, motilidade, vigor, concentração), estudo da morfologia dos espermatozóides, exames sorológicos(RIFI), parasitemia e hemogramas. Para tanto, colheitas de sangue e sêmen foram realizadas nos dia -2, -1, 1, 3, 5, 7, 9, 11, 14 e semanalmente até 84 dias pós-inoculação. Nenhuma alteração hematimétrica e nos parâmetros clínicos foi observada nos animais. Parasitemia foi detectada em um animal inoculado com oocistos, no 7o dia pós-infecção (DPI) e em outros dois suínos do GII (taquizoítos), nos 3o e 49o DPI.Exames sorológicos revelaram a presença de anticorpos contra T. gondii nos animais inoculados comoocistos ou taquizoítos a partir do 7º dia pós-infecção (DPI), com títulos de 256 e 64, alcançando pico de4096 nos dias 11 e 9 pós-inoculação, respectivamente. O GIII (controle) manteve-se negativo durantetodo o período experimental. Os parâmetros espermáticos avaliados não apresentaram nenhuma alteraçãoque pudesse ser atribuída a toxoplasmose. Diferenças significativas (P<0,05) quanto às patologiasespermáticas não foram observadas entre os grupos inoculados e o grupo controle. | ||
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10.5433/1679-0359.2004v25n2p107 doi (DE-627)DOAJ010842322 (DE-599)DOAJ6d00f5ba1b614c4384ce864527f8138e DE-627 ger DE-627 rakwb eng por S1-972 Alvimar José da Costa verfasserin aut Evaluation of semen parameters of boars (Sus scrofa) experimentally infected with Toxoplasma gondii/ <br< Avaliação dos parâmetros seminais de cachaços (Sus scrofa) experimentalmente infectados com Toxoplasma gondii 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aiming to investigate the influence of T. gondii on semen parameters and spermatozoa morphology, eight boars were inoculated with T. gondii. Experimental groups consisted of: GI (n=3) 1,5 x 104 oocysts of P strain; GII (n=3) 1,0 x 106 tachyzoites of RH strain and GIII (n=2), control noninoculated. Evaluations of semen parameters (volume, motility, strength, concentration), study of spermatozoa morphology, serology (RIFI), parasitemia and hemograms were performed. For this purpose, blood and semen collection were carried out on days -2, -1, 1, 3, 5, 7, 9, 11, 14 and weekly until 84 days post-inoculation. Non hematimetrics alterations and clinical signs were observed on animals. Parasitemia was detected in an animal inoculated with oocysts, on the 7th day post inoculation (DPI) and in a two pigs of GII (tachyzoites), on the 3rd and 49th DPI. Serology results revealed the presence of antibody anti-T. gondii on the animals inoculated with oocysts or tachyzoites since 7th DPI, with tittles of 256 and 64, reaching a maximal level of 4096 on days 11 and 9 post inoculation, respectively. The GIII (control) was negative through out all experimental period. The semen parametersevaluated did not present any alteration due to toxoplasmosis. Significative differences (P<0,05), considering spermatozoa pathology, were not observed between inoculated and control groups.<p<<p<Com o objetivo de investigar a influência do Toxoplasma gondii nos parâmetros seminais e na morfologia espermática de suínos, oito reprodutores foram inoculados com T. gondii, sendo constituídos os seguintes grupos experimentais: GI (n=3) 1,5 x 104 oocistos da cepa P, via oral; GII (n=3) 1,0 x 106 taquizoítos da cepa RH, via subcutânea e GIII (n=2), controle. Foram realizadas avaliações de parâmetros espermáticos (volume, motilidade, vigor, concentração), estudo da morfologia dos espermatozóides, exames sorológicos(RIFI), parasitemia e hemogramas. Para tanto, colheitas de sangue e sêmen foram realizadas nos dia -2, -1, 1, 3, 5, 7, 9, 11, 14 e semanalmente até 84 dias pós-inoculação. Nenhuma alteração hematimétrica e nos parâmetros clínicos foi observada nos animais. Parasitemia foi detectada em um animal inoculado com oocistos, no 7o dia pós-infecção (DPI) e em outros dois suínos do GII (taquizoítos), nos 3o e 49o DPI.Exames sorológicos revelaram a presença de anticorpos contra T. gondii nos animais inoculados comoocistos ou taquizoítos a partir do 7º dia pós-infecção (DPI), com títulos de 256 e 64, alcançando pico de4096 nos dias 11 e 9 pós-inoculação, respectivamente. O GIII (controle) manteve-se negativo durantetodo o período experimental. Os parâmetros espermáticos avaliados não apresentaram nenhuma alteraçãoque pudesse ser atribuída a toxoplasmose. Diferenças significativas (P<0,05) quanto às patologiasespermáticas não foram observadas entre os grupos inoculados e o grupo controle. Toxoplasmose Cachaço Parâmetros seminais Morfologia espermática. Agriculture (General) Fernanda de Rezende Pinto verfasserin aut Beatriz Bassora Paim verfasserin aut Daniela Cristina Di Mauro verfasserin aut Sérgio Jordão Filho verfasserin aut Anderson Barbosa de Moura verfasserin aut In Semina: Ciências Agrárias Universidade Estadual de Londrina, 2010 25(2004), 2, Seite 107-116 (DE-627)625424700 (DE-600)2551446-5 16790359 nnns volume:25 year:2004 number:2 pages:107-116 https://doi.org/10.5433/1679-0359.2004v25n2p107 kostenfrei https://doaj.org/article/6d00f5ba1b614c4384ce864527f8138e kostenfrei http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/2221 kostenfrei https://doaj.org/toc/1676-546X Journal toc kostenfrei https://doaj.org/toc/1679-0359 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 25 2004 2 107-116 |
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10.5433/1679-0359.2004v25n2p107 doi (DE-627)DOAJ010842322 (DE-599)DOAJ6d00f5ba1b614c4384ce864527f8138e DE-627 ger DE-627 rakwb eng por S1-972 Alvimar José da Costa verfasserin aut Evaluation of semen parameters of boars (Sus scrofa) experimentally infected with Toxoplasma gondii/ <br< Avaliação dos parâmetros seminais de cachaços (Sus scrofa) experimentalmente infectados com Toxoplasma gondii 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aiming to investigate the influence of T. gondii on semen parameters and spermatozoa morphology, eight boars were inoculated with T. gondii. Experimental groups consisted of: GI (n=3) 1,5 x 104 oocysts of P strain; GII (n=3) 1,0 x 106 tachyzoites of RH strain and GIII (n=2), control noninoculated. Evaluations of semen parameters (volume, motility, strength, concentration), study of spermatozoa morphology, serology (RIFI), parasitemia and hemograms were performed. For this purpose, blood and semen collection were carried out on days -2, -1, 1, 3, 5, 7, 9, 11, 14 and weekly until 84 days post-inoculation. Non hematimetrics alterations and clinical signs were observed on animals. Parasitemia was detected in an animal inoculated with oocysts, on the 7th day post inoculation (DPI) and in a two pigs of GII (tachyzoites), on the 3rd and 49th DPI. Serology results revealed the presence of antibody anti-T. gondii on the animals inoculated with oocysts or tachyzoites since 7th DPI, with tittles of 256 and 64, reaching a maximal level of 4096 on days 11 and 9 post inoculation, respectively. The GIII (control) was negative through out all experimental period. The semen parametersevaluated did not present any alteration due to toxoplasmosis. Significative differences (P<0,05), considering spermatozoa pathology, were not observed between inoculated and control groups.<p<<p<Com o objetivo de investigar a influência do Toxoplasma gondii nos parâmetros seminais e na morfologia espermática de suínos, oito reprodutores foram inoculados com T. gondii, sendo constituídos os seguintes grupos experimentais: GI (n=3) 1,5 x 104 oocistos da cepa P, via oral; GII (n=3) 1,0 x 106 taquizoítos da cepa RH, via subcutânea e GIII (n=2), controle. Foram realizadas avaliações de parâmetros espermáticos (volume, motilidade, vigor, concentração), estudo da morfologia dos espermatozóides, exames sorológicos(RIFI), parasitemia e hemogramas. Para tanto, colheitas de sangue e sêmen foram realizadas nos dia -2, -1, 1, 3, 5, 7, 9, 11, 14 e semanalmente até 84 dias pós-inoculação. Nenhuma alteração hematimétrica e nos parâmetros clínicos foi observada nos animais. Parasitemia foi detectada em um animal inoculado com oocistos, no 7o dia pós-infecção (DPI) e em outros dois suínos do GII (taquizoítos), nos 3o e 49o DPI.Exames sorológicos revelaram a presença de anticorpos contra T. gondii nos animais inoculados comoocistos ou taquizoítos a partir do 7º dia pós-infecção (DPI), com títulos de 256 e 64, alcançando pico de4096 nos dias 11 e 9 pós-inoculação, respectivamente. O GIII (controle) manteve-se negativo durantetodo o período experimental. Os parâmetros espermáticos avaliados não apresentaram nenhuma alteraçãoque pudesse ser atribuída a toxoplasmose. Diferenças significativas (P<0,05) quanto às patologiasespermáticas não foram observadas entre os grupos inoculados e o grupo controle. Toxoplasmose Cachaço Parâmetros seminais Morfologia espermática. Agriculture (General) Fernanda de Rezende Pinto verfasserin aut Beatriz Bassora Paim verfasserin aut Daniela Cristina Di Mauro verfasserin aut Sérgio Jordão Filho verfasserin aut Anderson Barbosa de Moura verfasserin aut In Semina: Ciências Agrárias Universidade Estadual de Londrina, 2010 25(2004), 2, Seite 107-116 (DE-627)625424700 (DE-600)2551446-5 16790359 nnns volume:25 year:2004 number:2 pages:107-116 https://doi.org/10.5433/1679-0359.2004v25n2p107 kostenfrei https://doaj.org/article/6d00f5ba1b614c4384ce864527f8138e kostenfrei http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/2221 kostenfrei https://doaj.org/toc/1676-546X Journal toc kostenfrei https://doaj.org/toc/1679-0359 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 25 2004 2 107-116 |
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10.5433/1679-0359.2004v25n2p107 doi (DE-627)DOAJ010842322 (DE-599)DOAJ6d00f5ba1b614c4384ce864527f8138e DE-627 ger DE-627 rakwb eng por S1-972 Alvimar José da Costa verfasserin aut Evaluation of semen parameters of boars (Sus scrofa) experimentally infected with Toxoplasma gondii/ <br< Avaliação dos parâmetros seminais de cachaços (Sus scrofa) experimentalmente infectados com Toxoplasma gondii 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aiming to investigate the influence of T. gondii on semen parameters and spermatozoa morphology, eight boars were inoculated with T. gondii. Experimental groups consisted of: GI (n=3) 1,5 x 104 oocysts of P strain; GII (n=3) 1,0 x 106 tachyzoites of RH strain and GIII (n=2), control noninoculated. Evaluations of semen parameters (volume, motility, strength, concentration), study of spermatozoa morphology, serology (RIFI), parasitemia and hemograms were performed. For this purpose, blood and semen collection were carried out on days -2, -1, 1, 3, 5, 7, 9, 11, 14 and weekly until 84 days post-inoculation. Non hematimetrics alterations and clinical signs were observed on animals. Parasitemia was detected in an animal inoculated with oocysts, on the 7th day post inoculation (DPI) and in a two pigs of GII (tachyzoites), on the 3rd and 49th DPI. Serology results revealed the presence of antibody anti-T. gondii on the animals inoculated with oocysts or tachyzoites since 7th DPI, with tittles of 256 and 64, reaching a maximal level of 4096 on days 11 and 9 post inoculation, respectively. The GIII (control) was negative through out all experimental period. The semen parametersevaluated did not present any alteration due to toxoplasmosis. Significative differences (P<0,05), considering spermatozoa pathology, were not observed between inoculated and control groups.<p<<p<Com o objetivo de investigar a influência do Toxoplasma gondii nos parâmetros seminais e na morfologia espermática de suínos, oito reprodutores foram inoculados com T. gondii, sendo constituídos os seguintes grupos experimentais: GI (n=3) 1,5 x 104 oocistos da cepa P, via oral; GII (n=3) 1,0 x 106 taquizoítos da cepa RH, via subcutânea e GIII (n=2), controle. Foram realizadas avaliações de parâmetros espermáticos (volume, motilidade, vigor, concentração), estudo da morfologia dos espermatozóides, exames sorológicos(RIFI), parasitemia e hemogramas. Para tanto, colheitas de sangue e sêmen foram realizadas nos dia -2, -1, 1, 3, 5, 7, 9, 11, 14 e semanalmente até 84 dias pós-inoculação. Nenhuma alteração hematimétrica e nos parâmetros clínicos foi observada nos animais. Parasitemia foi detectada em um animal inoculado com oocistos, no 7o dia pós-infecção (DPI) e em outros dois suínos do GII (taquizoítos), nos 3o e 49o DPI.Exames sorológicos revelaram a presença de anticorpos contra T. gondii nos animais inoculados comoocistos ou taquizoítos a partir do 7º dia pós-infecção (DPI), com títulos de 256 e 64, alcançando pico de4096 nos dias 11 e 9 pós-inoculação, respectivamente. O GIII (controle) manteve-se negativo durantetodo o período experimental. Os parâmetros espermáticos avaliados não apresentaram nenhuma alteraçãoque pudesse ser atribuída a toxoplasmose. Diferenças significativas (P<0,05) quanto às patologiasespermáticas não foram observadas entre os grupos inoculados e o grupo controle. Toxoplasmose Cachaço Parâmetros seminais Morfologia espermática. Agriculture (General) Fernanda de Rezende Pinto verfasserin aut Beatriz Bassora Paim verfasserin aut Daniela Cristina Di Mauro verfasserin aut Sérgio Jordão Filho verfasserin aut Anderson Barbosa de Moura verfasserin aut In Semina: Ciências Agrárias Universidade Estadual de Londrina, 2010 25(2004), 2, Seite 107-116 (DE-627)625424700 (DE-600)2551446-5 16790359 nnns volume:25 year:2004 number:2 pages:107-116 https://doi.org/10.5433/1679-0359.2004v25n2p107 kostenfrei https://doaj.org/article/6d00f5ba1b614c4384ce864527f8138e kostenfrei http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/2221 kostenfrei https://doaj.org/toc/1676-546X Journal toc kostenfrei https://doaj.org/toc/1679-0359 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 25 2004 2 107-116 |
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10.5433/1679-0359.2004v25n2p107 doi (DE-627)DOAJ010842322 (DE-599)DOAJ6d00f5ba1b614c4384ce864527f8138e DE-627 ger DE-627 rakwb eng por S1-972 Alvimar José da Costa verfasserin aut Evaluation of semen parameters of boars (Sus scrofa) experimentally infected with Toxoplasma gondii/ <br< Avaliação dos parâmetros seminais de cachaços (Sus scrofa) experimentalmente infectados com Toxoplasma gondii 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aiming to investigate the influence of T. gondii on semen parameters and spermatozoa morphology, eight boars were inoculated with T. gondii. Experimental groups consisted of: GI (n=3) 1,5 x 104 oocysts of P strain; GII (n=3) 1,0 x 106 tachyzoites of RH strain and GIII (n=2), control noninoculated. Evaluations of semen parameters (volume, motility, strength, concentration), study of spermatozoa morphology, serology (RIFI), parasitemia and hemograms were performed. For this purpose, blood and semen collection were carried out on days -2, -1, 1, 3, 5, 7, 9, 11, 14 and weekly until 84 days post-inoculation. Non hematimetrics alterations and clinical signs were observed on animals. Parasitemia was detected in an animal inoculated with oocysts, on the 7th day post inoculation (DPI) and in a two pigs of GII (tachyzoites), on the 3rd and 49th DPI. Serology results revealed the presence of antibody anti-T. gondii on the animals inoculated with oocysts or tachyzoites since 7th DPI, with tittles of 256 and 64, reaching a maximal level of 4096 on days 11 and 9 post inoculation, respectively. The GIII (control) was negative through out all experimental period. The semen parametersevaluated did not present any alteration due to toxoplasmosis. Significative differences (P<0,05), considering spermatozoa pathology, were not observed between inoculated and control groups.<p<<p<Com o objetivo de investigar a influência do Toxoplasma gondii nos parâmetros seminais e na morfologia espermática de suínos, oito reprodutores foram inoculados com T. gondii, sendo constituídos os seguintes grupos experimentais: GI (n=3) 1,5 x 104 oocistos da cepa P, via oral; GII (n=3) 1,0 x 106 taquizoítos da cepa RH, via subcutânea e GIII (n=2), controle. Foram realizadas avaliações de parâmetros espermáticos (volume, motilidade, vigor, concentração), estudo da morfologia dos espermatozóides, exames sorológicos(RIFI), parasitemia e hemogramas. Para tanto, colheitas de sangue e sêmen foram realizadas nos dia -2, -1, 1, 3, 5, 7, 9, 11, 14 e semanalmente até 84 dias pós-inoculação. Nenhuma alteração hematimétrica e nos parâmetros clínicos foi observada nos animais. Parasitemia foi detectada em um animal inoculado com oocistos, no 7o dia pós-infecção (DPI) e em outros dois suínos do GII (taquizoítos), nos 3o e 49o DPI.Exames sorológicos revelaram a presença de anticorpos contra T. gondii nos animais inoculados comoocistos ou taquizoítos a partir do 7º dia pós-infecção (DPI), com títulos de 256 e 64, alcançando pico de4096 nos dias 11 e 9 pós-inoculação, respectivamente. O GIII (controle) manteve-se negativo durantetodo o período experimental. Os parâmetros espermáticos avaliados não apresentaram nenhuma alteraçãoque pudesse ser atribuída a toxoplasmose. Diferenças significativas (P<0,05) quanto às patologiasespermáticas não foram observadas entre os grupos inoculados e o grupo controle. Toxoplasmose Cachaço Parâmetros seminais Morfologia espermática. Agriculture (General) Fernanda de Rezende Pinto verfasserin aut Beatriz Bassora Paim verfasserin aut Daniela Cristina Di Mauro verfasserin aut Sérgio Jordão Filho verfasserin aut Anderson Barbosa de Moura verfasserin aut In Semina: Ciências Agrárias Universidade Estadual de Londrina, 2010 25(2004), 2, Seite 107-116 (DE-627)625424700 (DE-600)2551446-5 16790359 nnns volume:25 year:2004 number:2 pages:107-116 https://doi.org/10.5433/1679-0359.2004v25n2p107 kostenfrei https://doaj.org/article/6d00f5ba1b614c4384ce864527f8138e kostenfrei http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/2221 kostenfrei https://doaj.org/toc/1676-546X Journal toc kostenfrei https://doaj.org/toc/1679-0359 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 25 2004 2 107-116 |
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In Semina: Ciências Agrárias 25(2004), 2, Seite 107-116 volume:25 year:2004 number:2 pages:107-116 |
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Alvimar José da Costa @@aut@@ Fernanda de Rezende Pinto @@aut@@ Beatriz Bassora Paim @@aut@@ Daniela Cristina Di Mauro @@aut@@ Sérgio Jordão Filho @@aut@@ Anderson Barbosa de Moura @@aut@@ |
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2004-01-01T00:00:00Z |
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S1-972 Evaluation of semen parameters of boars (Sus scrofa) experimentally infected with Toxoplasma gondii/ <br< Avaliação dos parâmetros seminais de cachaços (Sus scrofa) experimentalmente infectados com Toxoplasma gondii Toxoplasmose Cachaço Parâmetros seminais Morfologia espermática |
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Evaluation of semen parameters of boars (Sus scrofa) experimentally infected with Toxoplasma gondii/ <br< Avaliação dos parâmetros seminais de cachaços (Sus scrofa) experimentalmente infectados com Toxoplasma gondii |
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Evaluation of semen parameters of boars (Sus scrofa) experimentally infected with Toxoplasma gondii/ <br< Avaliação dos parâmetros seminais de cachaços (Sus scrofa) experimentalmente infectados com Toxoplasma gondii |
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Alvimar José da Costa Fernanda de Rezende Pinto Beatriz Bassora Paim Daniela Cristina Di Mauro Sérgio Jordão Filho Anderson Barbosa de Moura |
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evaluation of semen parameters of boars (sus scrofa) experimentally infected with toxoplasma gondii/ <br< avaliação dos parâmetros seminais de cachaços (sus scrofa) experimentalmente infectados com toxoplasma gondii |
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Evaluation of semen parameters of boars (Sus scrofa) experimentally infected with Toxoplasma gondii/ <br< Avaliação dos parâmetros seminais de cachaços (Sus scrofa) experimentalmente infectados com Toxoplasma gondii |
abstract |
Aiming to investigate the influence of T. gondii on semen parameters and spermatozoa morphology, eight boars were inoculated with T. gondii. Experimental groups consisted of: GI (n=3) 1,5 x 104 oocysts of P strain; GII (n=3) 1,0 x 106 tachyzoites of RH strain and GIII (n=2), control noninoculated. Evaluations of semen parameters (volume, motility, strength, concentration), study of spermatozoa morphology, serology (RIFI), parasitemia and hemograms were performed. For this purpose, blood and semen collection were carried out on days -2, -1, 1, 3, 5, 7, 9, 11, 14 and weekly until 84 days post-inoculation. Non hematimetrics alterations and clinical signs were observed on animals. Parasitemia was detected in an animal inoculated with oocysts, on the 7th day post inoculation (DPI) and in a two pigs of GII (tachyzoites), on the 3rd and 49th DPI. Serology results revealed the presence of antibody anti-T. gondii on the animals inoculated with oocysts or tachyzoites since 7th DPI, with tittles of 256 and 64, reaching a maximal level of 4096 on days 11 and 9 post inoculation, respectively. The GIII (control) was negative through out all experimental period. The semen parametersevaluated did not present any alteration due to toxoplasmosis. Significative differences (P<0,05), considering spermatozoa pathology, were not observed between inoculated and control groups.<p<<p<Com o objetivo de investigar a influência do Toxoplasma gondii nos parâmetros seminais e na morfologia espermática de suínos, oito reprodutores foram inoculados com T. gondii, sendo constituídos os seguintes grupos experimentais: GI (n=3) 1,5 x 104 oocistos da cepa P, via oral; GII (n=3) 1,0 x 106 taquizoítos da cepa RH, via subcutânea e GIII (n=2), controle. Foram realizadas avaliações de parâmetros espermáticos (volume, motilidade, vigor, concentração), estudo da morfologia dos espermatozóides, exames sorológicos(RIFI), parasitemia e hemogramas. Para tanto, colheitas de sangue e sêmen foram realizadas nos dia -2, -1, 1, 3, 5, 7, 9, 11, 14 e semanalmente até 84 dias pós-inoculação. Nenhuma alteração hematimétrica e nos parâmetros clínicos foi observada nos animais. Parasitemia foi detectada em um animal inoculado com oocistos, no 7o dia pós-infecção (DPI) e em outros dois suínos do GII (taquizoítos), nos 3o e 49o DPI.Exames sorológicos revelaram a presença de anticorpos contra T. gondii nos animais inoculados comoocistos ou taquizoítos a partir do 7º dia pós-infecção (DPI), com títulos de 256 e 64, alcançando pico de4096 nos dias 11 e 9 pós-inoculação, respectivamente. O GIII (controle) manteve-se negativo durantetodo o período experimental. Os parâmetros espermáticos avaliados não apresentaram nenhuma alteraçãoque pudesse ser atribuída a toxoplasmose. Diferenças significativas (P<0,05) quanto às patologiasespermáticas não foram observadas entre os grupos inoculados e o grupo controle. |
abstractGer |
Aiming to investigate the influence of T. gondii on semen parameters and spermatozoa morphology, eight boars were inoculated with T. gondii. Experimental groups consisted of: GI (n=3) 1,5 x 104 oocysts of P strain; GII (n=3) 1,0 x 106 tachyzoites of RH strain and GIII (n=2), control noninoculated. Evaluations of semen parameters (volume, motility, strength, concentration), study of spermatozoa morphology, serology (RIFI), parasitemia and hemograms were performed. For this purpose, blood and semen collection were carried out on days -2, -1, 1, 3, 5, 7, 9, 11, 14 and weekly until 84 days post-inoculation. Non hematimetrics alterations and clinical signs were observed on animals. Parasitemia was detected in an animal inoculated with oocysts, on the 7th day post inoculation (DPI) and in a two pigs of GII (tachyzoites), on the 3rd and 49th DPI. Serology results revealed the presence of antibody anti-T. gondii on the animals inoculated with oocysts or tachyzoites since 7th DPI, with tittles of 256 and 64, reaching a maximal level of 4096 on days 11 and 9 post inoculation, respectively. The GIII (control) was negative through out all experimental period. The semen parametersevaluated did not present any alteration due to toxoplasmosis. Significative differences (P<0,05), considering spermatozoa pathology, were not observed between inoculated and control groups.<p<<p<Com o objetivo de investigar a influência do Toxoplasma gondii nos parâmetros seminais e na morfologia espermática de suínos, oito reprodutores foram inoculados com T. gondii, sendo constituídos os seguintes grupos experimentais: GI (n=3) 1,5 x 104 oocistos da cepa P, via oral; GII (n=3) 1,0 x 106 taquizoítos da cepa RH, via subcutânea e GIII (n=2), controle. Foram realizadas avaliações de parâmetros espermáticos (volume, motilidade, vigor, concentração), estudo da morfologia dos espermatozóides, exames sorológicos(RIFI), parasitemia e hemogramas. Para tanto, colheitas de sangue e sêmen foram realizadas nos dia -2, -1, 1, 3, 5, 7, 9, 11, 14 e semanalmente até 84 dias pós-inoculação. Nenhuma alteração hematimétrica e nos parâmetros clínicos foi observada nos animais. Parasitemia foi detectada em um animal inoculado com oocistos, no 7o dia pós-infecção (DPI) e em outros dois suínos do GII (taquizoítos), nos 3o e 49o DPI.Exames sorológicos revelaram a presença de anticorpos contra T. gondii nos animais inoculados comoocistos ou taquizoítos a partir do 7º dia pós-infecção (DPI), com títulos de 256 e 64, alcançando pico de4096 nos dias 11 e 9 pós-inoculação, respectivamente. O GIII (controle) manteve-se negativo durantetodo o período experimental. Os parâmetros espermáticos avaliados não apresentaram nenhuma alteraçãoque pudesse ser atribuída a toxoplasmose. Diferenças significativas (P<0,05) quanto às patologiasespermáticas não foram observadas entre os grupos inoculados e o grupo controle. |
abstract_unstemmed |
Aiming to investigate the influence of T. gondii on semen parameters and spermatozoa morphology, eight boars were inoculated with T. gondii. Experimental groups consisted of: GI (n=3) 1,5 x 104 oocysts of P strain; GII (n=3) 1,0 x 106 tachyzoites of RH strain and GIII (n=2), control noninoculated. Evaluations of semen parameters (volume, motility, strength, concentration), study of spermatozoa morphology, serology (RIFI), parasitemia and hemograms were performed. For this purpose, blood and semen collection were carried out on days -2, -1, 1, 3, 5, 7, 9, 11, 14 and weekly until 84 days post-inoculation. Non hematimetrics alterations and clinical signs were observed on animals. Parasitemia was detected in an animal inoculated with oocysts, on the 7th day post inoculation (DPI) and in a two pigs of GII (tachyzoites), on the 3rd and 49th DPI. Serology results revealed the presence of antibody anti-T. gondii on the animals inoculated with oocysts or tachyzoites since 7th DPI, with tittles of 256 and 64, reaching a maximal level of 4096 on days 11 and 9 post inoculation, respectively. The GIII (control) was negative through out all experimental period. The semen parametersevaluated did not present any alteration due to toxoplasmosis. Significative differences (P<0,05), considering spermatozoa pathology, were not observed between inoculated and control groups.<p<<p<Com o objetivo de investigar a influência do Toxoplasma gondii nos parâmetros seminais e na morfologia espermática de suínos, oito reprodutores foram inoculados com T. gondii, sendo constituídos os seguintes grupos experimentais: GI (n=3) 1,5 x 104 oocistos da cepa P, via oral; GII (n=3) 1,0 x 106 taquizoítos da cepa RH, via subcutânea e GIII (n=2), controle. Foram realizadas avaliações de parâmetros espermáticos (volume, motilidade, vigor, concentração), estudo da morfologia dos espermatozóides, exames sorológicos(RIFI), parasitemia e hemogramas. Para tanto, colheitas de sangue e sêmen foram realizadas nos dia -2, -1, 1, 3, 5, 7, 9, 11, 14 e semanalmente até 84 dias pós-inoculação. Nenhuma alteração hematimétrica e nos parâmetros clínicos foi observada nos animais. Parasitemia foi detectada em um animal inoculado com oocistos, no 7o dia pós-infecção (DPI) e em outros dois suínos do GII (taquizoítos), nos 3o e 49o DPI.Exames sorológicos revelaram a presença de anticorpos contra T. gondii nos animais inoculados comoocistos ou taquizoítos a partir do 7º dia pós-infecção (DPI), com títulos de 256 e 64, alcançando pico de4096 nos dias 11 e 9 pós-inoculação, respectivamente. O GIII (controle) manteve-se negativo durantetodo o período experimental. Os parâmetros espermáticos avaliados não apresentaram nenhuma alteraçãoque pudesse ser atribuída a toxoplasmose. Diferenças significativas (P<0,05) quanto às patologiasespermáticas não foram observadas entre os grupos inoculados e o grupo controle. |
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Evaluation of semen parameters of boars (Sus scrofa) experimentally infected with Toxoplasma gondii/ <br< Avaliação dos parâmetros seminais de cachaços (Sus scrofa) experimentalmente infectados com Toxoplasma gondii |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ010842322</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230310031925.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230225s2004 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.5433/1679-0359.2004v25n2p107</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ010842322</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ6d00f5ba1b614c4384ce864527f8138e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield><subfield code="a">por</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">S1-972</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Alvimar José da Costa</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Evaluation of semen parameters of boars (Sus scrofa) experimentally infected with Toxoplasma gondii/ <br< Avaliação dos parâmetros seminais de cachaços (Sus scrofa) experimentalmente infectados com Toxoplasma gondii</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2004</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Aiming to investigate the influence of T. gondii on semen parameters and spermatozoa morphology, eight boars were inoculated with T. gondii. Experimental groups consisted of: GI (n=3) 1,5 x 104 oocysts of P strain; GII (n=3) 1,0 x 106 tachyzoites of RH strain and GIII (n=2), control noninoculated. Evaluations of semen parameters (volume, motility, strength, concentration), study of spermatozoa morphology, serology (RIFI), parasitemia and hemograms were performed. For this purpose, blood and semen collection were carried out on days -2, -1, 1, 3, 5, 7, 9, 11, 14 and weekly until 84 days post-inoculation. Non hematimetrics alterations and clinical signs were observed on animals. Parasitemia was detected in an animal inoculated with oocysts, on the 7th day post inoculation (DPI) and in a two pigs of GII (tachyzoites), on the 3rd and 49th DPI. Serology results revealed the presence of antibody anti-T. gondii on the animals inoculated with oocysts or tachyzoites since 7th DPI, with tittles of 256 and 64, reaching a maximal level of 4096 on days 11 and 9 post inoculation, respectively. The GIII (control) was negative through out all experimental period. The semen parametersevaluated did not present any alteration due to toxoplasmosis. Significative differences (P<0,05), considering spermatozoa pathology, were not observed between inoculated and control groups.<p<<p<Com o objetivo de investigar a influência do Toxoplasma gondii nos parâmetros seminais e na morfologia espermática de suínos, oito reprodutores foram inoculados com T. gondii, sendo constituídos os seguintes grupos experimentais: GI (n=3) 1,5 x 104 oocistos da cepa P, via oral; GII (n=3) 1,0 x 106 taquizoítos da cepa RH, via subcutânea e GIII (n=2), controle. Foram realizadas avaliações de parâmetros espermáticos (volume, motilidade, vigor, concentração), estudo da morfologia dos espermatozóides, exames sorológicos(RIFI), parasitemia e hemogramas. Para tanto, colheitas de sangue e sêmen foram realizadas nos dia -2, -1, 1, 3, 5, 7, 9, 11, 14 e semanalmente até 84 dias pós-inoculação. Nenhuma alteração hematimétrica e nos parâmetros clínicos foi observada nos animais. Parasitemia foi detectada em um animal inoculado com oocistos, no 7o dia pós-infecção (DPI) e em outros dois suínos do GII (taquizoítos), nos 3o e 49o DPI.Exames sorológicos revelaram a presença de anticorpos contra T. gondii nos animais inoculados comoocistos ou taquizoítos a partir do 7º dia pós-infecção (DPI), com títulos de 256 e 64, alcançando pico de4096 nos dias 11 e 9 pós-inoculação, respectivamente. O GIII (controle) manteve-se negativo durantetodo o período experimental. Os parâmetros espermáticos avaliados não apresentaram nenhuma alteraçãoque pudesse ser atribuída a toxoplasmose. Diferenças significativas (P<0,05) quanto às patologiasespermáticas não foram observadas entre os grupos inoculados e o grupo controle.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Toxoplasmose</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cachaço</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Parâmetros seminais</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Morfologia espermática.</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Agriculture (General)</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Fernanda de Rezende Pinto</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Beatriz Bassora Paim</subfield><subfield code="e">verfasserin</subfield><subfield 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