Aspergillus piperis A/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen Pythium aphanidermatum

Adding compost to soil can result in plant disease suppression through the mechanisms of antagonistic action of compost microflora against plant pathogens. The aim of the study was to select effective antagonists of Pythium aphanidermatum from compost, to assess the effect of its extracellular metab...
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Autor*in:	Jovičić-Petrović Jelena [verfasserIn] Jeremić Sanja [verfasserIn] Vučković Ivan [verfasserIn] Vojnović Sandra [verfasserIn] Bulajić Aleksandra [verfasserIn] Raičević Vera [verfasserIn] Nikodinovic-Runic Jasmina [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	antifungal activity antifungal proteins Aspergillus piperis organic acids Pythium aphanidermatum compost

Übergeordnetes Werk:	In: Archives of Biological Sciences - University of Belgrade, University of Novi Sad, 2010, 68(2016), 2, Seite 279-289
Übergeordnetes Werk:	volume:68 ; year:2016 ; number:2 ; pages:279-289

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.2298/ABS150602016J

Katalog-ID:	DOAJ011975105

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allfields	10.2298/ABS150602016J doi (DE-627)DOAJ011975105 (DE-599)DOAJe541db437c0644aba8b02f7142cd7a6e DE-627 ger DE-627 rakwb eng QH301-705.5 Jovičić-Petrović Jelena verfasserin aut Aspergillus piperis A/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen Pythium aphanidermatum 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Adding compost to soil can result in plant disease suppression through the mechanisms of antagonistic action of compost microflora against plant pathogens. The aim of the study was to select effective antagonists of Pythium aphanidermatum from compost, to assess the effect of its extracellular metabolites on the plant pathogen, and to characterize antifungal metabolites. The fungal isolate selected by a confrontation test was identified as Aspergillus piperis A/5 on the basis of morphological features and the internal transcribed spacer (ITS) region, β-tubulin and calmodulin partial sequences. Liquid chromatography-mass spectroscopy (LC-MS) analysis showed that gluconic and citric acid were the most abundant in the organic culture extract. However, the main antifungal activity was contained in the aqueous phase remaining after the organic solvent extraction. The presence of considerable amounts of proteins in both the crude culture extract as well as the aqueous phase remaining after solvent extraction was confirmed by SDS-PAGE. Isolated Aspergillus piperis A/5 exhibits strong antifungal activity against the phytopathogen Pythium aphanidermatum. It secretes a complex mixture of metabolites consisting of small molecules, including gluconic acid, citric acid and itaconic acid derivatives, but the most potent antifungal activity was associated with proteins resistant to heat and organic solvents. Our findings about the activity and characterization of antagonistic strain metabolites contribute to the understanding of the mechanism of interaction of antifungal metabolites as well as fungal-fungal interaction. The obtained results provide a basis for further application development in agriculture and food processing. [Projekat Ministarstva nauke Republike Srbije, br. TR31080 i br. ON173048, i EU Commission project AREA, br.316004] antifungal activity antifungal proteins Aspergillus piperis organic acids Pythium aphanidermatum compost Biology (General) Jeremić Sanja verfasserin aut Vučković Ivan verfasserin aut Vojnović Sandra verfasserin aut Bulajić Aleksandra verfasserin aut Raičević Vera verfasserin aut Nikodinovic-Runic Jasmina verfasserin aut In Archives of Biological Sciences University of Belgrade, University of Novi Sad, 2010 68(2016), 2, Seite 279-289 (DE-627)538998008 (DE-600)2380842-1 18214339 nnns volume:68 year:2016 number:2 pages:279-289 https://doi.org/10.2298/ABS150602016J kostenfrei https://doaj.org/article/e541db437c0644aba8b02f7142cd7a6e kostenfrei http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600016J.pdf kostenfrei https://doaj.org/toc/0354-4664 Journal toc kostenfrei https://doaj.org/toc/1821-4339 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 68 2016 2 279-289
spelling	10.2298/ABS150602016J doi (DE-627)DOAJ011975105 (DE-599)DOAJe541db437c0644aba8b02f7142cd7a6e DE-627 ger DE-627 rakwb eng QH301-705.5 Jovičić-Petrović Jelena verfasserin aut Aspergillus piperis A/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen Pythium aphanidermatum 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Adding compost to soil can result in plant disease suppression through the mechanisms of antagonistic action of compost microflora against plant pathogens. The aim of the study was to select effective antagonists of Pythium aphanidermatum from compost, to assess the effect of its extracellular metabolites on the plant pathogen, and to characterize antifungal metabolites. The fungal isolate selected by a confrontation test was identified as Aspergillus piperis A/5 on the basis of morphological features and the internal transcribed spacer (ITS) region, β-tubulin and calmodulin partial sequences. Liquid chromatography-mass spectroscopy (LC-MS) analysis showed that gluconic and citric acid were the most abundant in the organic culture extract. However, the main antifungal activity was contained in the aqueous phase remaining after the organic solvent extraction. The presence of considerable amounts of proteins in both the crude culture extract as well as the aqueous phase remaining after solvent extraction was confirmed by SDS-PAGE. Isolated Aspergillus piperis A/5 exhibits strong antifungal activity against the phytopathogen Pythium aphanidermatum. It secretes a complex mixture of metabolites consisting of small molecules, including gluconic acid, citric acid and itaconic acid derivatives, but the most potent antifungal activity was associated with proteins resistant to heat and organic solvents. Our findings about the activity and characterization of antagonistic strain metabolites contribute to the understanding of the mechanism of interaction of antifungal metabolites as well as fungal-fungal interaction. The obtained results provide a basis for further application development in agriculture and food processing. [Projekat Ministarstva nauke Republike Srbije, br. TR31080 i br. ON173048, i EU Commission project AREA, br.316004] antifungal activity antifungal proteins Aspergillus piperis organic acids Pythium aphanidermatum compost Biology (General) Jeremić Sanja verfasserin aut Vučković Ivan verfasserin aut Vojnović Sandra verfasserin aut Bulajić Aleksandra verfasserin aut Raičević Vera verfasserin aut Nikodinovic-Runic Jasmina verfasserin aut In Archives of Biological Sciences University of Belgrade, University of Novi Sad, 2010 68(2016), 2, Seite 279-289 (DE-627)538998008 (DE-600)2380842-1 18214339 nnns volume:68 year:2016 number:2 pages:279-289 https://doi.org/10.2298/ABS150602016J kostenfrei https://doaj.org/article/e541db437c0644aba8b02f7142cd7a6e kostenfrei http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600016J.pdf kostenfrei https://doaj.org/toc/0354-4664 Journal toc kostenfrei https://doaj.org/toc/1821-4339 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 68 2016 2 279-289
allfields_unstemmed	10.2298/ABS150602016J doi (DE-627)DOAJ011975105 (DE-599)DOAJe541db437c0644aba8b02f7142cd7a6e DE-627 ger DE-627 rakwb eng QH301-705.5 Jovičić-Petrović Jelena verfasserin aut Aspergillus piperis A/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen Pythium aphanidermatum 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Adding compost to soil can result in plant disease suppression through the mechanisms of antagonistic action of compost microflora against plant pathogens. The aim of the study was to select effective antagonists of Pythium aphanidermatum from compost, to assess the effect of its extracellular metabolites on the plant pathogen, and to characterize antifungal metabolites. The fungal isolate selected by a confrontation test was identified as Aspergillus piperis A/5 on the basis of morphological features and the internal transcribed spacer (ITS) region, β-tubulin and calmodulin partial sequences. Liquid chromatography-mass spectroscopy (LC-MS) analysis showed that gluconic and citric acid were the most abundant in the organic culture extract. However, the main antifungal activity was contained in the aqueous phase remaining after the organic solvent extraction. The presence of considerable amounts of proteins in both the crude culture extract as well as the aqueous phase remaining after solvent extraction was confirmed by SDS-PAGE. Isolated Aspergillus piperis A/5 exhibits strong antifungal activity against the phytopathogen Pythium aphanidermatum. It secretes a complex mixture of metabolites consisting of small molecules, including gluconic acid, citric acid and itaconic acid derivatives, but the most potent antifungal activity was associated with proteins resistant to heat and organic solvents. Our findings about the activity and characterization of antagonistic strain metabolites contribute to the understanding of the mechanism of interaction of antifungal metabolites as well as fungal-fungal interaction. The obtained results provide a basis for further application development in agriculture and food processing. [Projekat Ministarstva nauke Republike Srbije, br. TR31080 i br. ON173048, i EU Commission project AREA, br.316004] antifungal activity antifungal proteins Aspergillus piperis organic acids Pythium aphanidermatum compost Biology (General) Jeremić Sanja verfasserin aut Vučković Ivan verfasserin aut Vojnović Sandra verfasserin aut Bulajić Aleksandra verfasserin aut Raičević Vera verfasserin aut Nikodinovic-Runic Jasmina verfasserin aut In Archives of Biological Sciences University of Belgrade, University of Novi Sad, 2010 68(2016), 2, Seite 279-289 (DE-627)538998008 (DE-600)2380842-1 18214339 nnns volume:68 year:2016 number:2 pages:279-289 https://doi.org/10.2298/ABS150602016J kostenfrei https://doaj.org/article/e541db437c0644aba8b02f7142cd7a6e kostenfrei http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600016J.pdf kostenfrei https://doaj.org/toc/0354-4664 Journal toc kostenfrei https://doaj.org/toc/1821-4339 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 68 2016 2 279-289
allfieldsGer	10.2298/ABS150602016J doi (DE-627)DOAJ011975105 (DE-599)DOAJe541db437c0644aba8b02f7142cd7a6e DE-627 ger DE-627 rakwb eng QH301-705.5 Jovičić-Petrović Jelena verfasserin aut Aspergillus piperis A/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen Pythium aphanidermatum 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Adding compost to soil can result in plant disease suppression through the mechanisms of antagonistic action of compost microflora against plant pathogens. The aim of the study was to select effective antagonists of Pythium aphanidermatum from compost, to assess the effect of its extracellular metabolites on the plant pathogen, and to characterize antifungal metabolites. The fungal isolate selected by a confrontation test was identified as Aspergillus piperis A/5 on the basis of morphological features and the internal transcribed spacer (ITS) region, β-tubulin and calmodulin partial sequences. Liquid chromatography-mass spectroscopy (LC-MS) analysis showed that gluconic and citric acid were the most abundant in the organic culture extract. However, the main antifungal activity was contained in the aqueous phase remaining after the organic solvent extraction. The presence of considerable amounts of proteins in both the crude culture extract as well as the aqueous phase remaining after solvent extraction was confirmed by SDS-PAGE. Isolated Aspergillus piperis A/5 exhibits strong antifungal activity against the phytopathogen Pythium aphanidermatum. It secretes a complex mixture of metabolites consisting of small molecules, including gluconic acid, citric acid and itaconic acid derivatives, but the most potent antifungal activity was associated with proteins resistant to heat and organic solvents. Our findings about the activity and characterization of antagonistic strain metabolites contribute to the understanding of the mechanism of interaction of antifungal metabolites as well as fungal-fungal interaction. The obtained results provide a basis for further application development in agriculture and food processing. [Projekat Ministarstva nauke Republike Srbije, br. TR31080 i br. ON173048, i EU Commission project AREA, br.316004] antifungal activity antifungal proteins Aspergillus piperis organic acids Pythium aphanidermatum compost Biology (General) Jeremić Sanja verfasserin aut Vučković Ivan verfasserin aut Vojnović Sandra verfasserin aut Bulajić Aleksandra verfasserin aut Raičević Vera verfasserin aut Nikodinovic-Runic Jasmina verfasserin aut In Archives of Biological Sciences University of Belgrade, University of Novi Sad, 2010 68(2016), 2, Seite 279-289 (DE-627)538998008 (DE-600)2380842-1 18214339 nnns volume:68 year:2016 number:2 pages:279-289 https://doi.org/10.2298/ABS150602016J kostenfrei https://doaj.org/article/e541db437c0644aba8b02f7142cd7a6e kostenfrei http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600016J.pdf kostenfrei https://doaj.org/toc/0354-4664 Journal toc kostenfrei https://doaj.org/toc/1821-4339 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 68 2016 2 279-289
allfieldsSound	10.2298/ABS150602016J doi (DE-627)DOAJ011975105 (DE-599)DOAJe541db437c0644aba8b02f7142cd7a6e DE-627 ger DE-627 rakwb eng QH301-705.5 Jovičić-Petrović Jelena verfasserin aut Aspergillus piperis A/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen Pythium aphanidermatum 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Adding compost to soil can result in plant disease suppression through the mechanisms of antagonistic action of compost microflora against plant pathogens. The aim of the study was to select effective antagonists of Pythium aphanidermatum from compost, to assess the effect of its extracellular metabolites on the plant pathogen, and to characterize antifungal metabolites. The fungal isolate selected by a confrontation test was identified as Aspergillus piperis A/5 on the basis of morphological features and the internal transcribed spacer (ITS) region, β-tubulin and calmodulin partial sequences. Liquid chromatography-mass spectroscopy (LC-MS) analysis showed that gluconic and citric acid were the most abundant in the organic culture extract. However, the main antifungal activity was contained in the aqueous phase remaining after the organic solvent extraction. The presence of considerable amounts of proteins in both the crude culture extract as well as the aqueous phase remaining after solvent extraction was confirmed by SDS-PAGE. Isolated Aspergillus piperis A/5 exhibits strong antifungal activity against the phytopathogen Pythium aphanidermatum. It secretes a complex mixture of metabolites consisting of small molecules, including gluconic acid, citric acid and itaconic acid derivatives, but the most potent antifungal activity was associated with proteins resistant to heat and organic solvents. Our findings about the activity and characterization of antagonistic strain metabolites contribute to the understanding of the mechanism of interaction of antifungal metabolites as well as fungal-fungal interaction. The obtained results provide a basis for further application development in agriculture and food processing. [Projekat Ministarstva nauke Republike Srbije, br. TR31080 i br. ON173048, i EU Commission project AREA, br.316004] antifungal activity antifungal proteins Aspergillus piperis organic acids Pythium aphanidermatum compost Biology (General) Jeremić Sanja verfasserin aut Vučković Ivan verfasserin aut Vojnović Sandra verfasserin aut Bulajić Aleksandra verfasserin aut Raičević Vera verfasserin aut Nikodinovic-Runic Jasmina verfasserin aut In Archives of Biological Sciences University of Belgrade, University of Novi Sad, 2010 68(2016), 2, Seite 279-289 (DE-627)538998008 (DE-600)2380842-1 18214339 nnns volume:68 year:2016 number:2 pages:279-289 https://doi.org/10.2298/ABS150602016J kostenfrei https://doaj.org/article/e541db437c0644aba8b02f7142cd7a6e kostenfrei http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600016J.pdf kostenfrei https://doaj.org/toc/0354-4664 Journal toc kostenfrei https://doaj.org/toc/1821-4339 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 68 2016 2 279-289
language	English
source	In Archives of Biological Sciences 68(2016), 2, Seite 279-289 volume:68 year:2016 number:2 pages:279-289
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topic_facet	antifungal activity antifungal proteins Aspergillus piperis organic acids Pythium aphanidermatum compost Biology (General)
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authorswithroles_txt_mv	Jovičić-Petrović Jelena @@aut@@ Jeremić Sanja @@aut@@ Vučković Ivan @@aut@@ Vojnović Sandra @@aut@@ Bulajić Aleksandra @@aut@@ Raičević Vera @@aut@@ Nikodinovic-Runic Jasmina @@aut@@
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topic_title	QH301-705.5 Aspergillus piperis A/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen Pythium aphanidermatum antifungal activity antifungal proteins Aspergillus piperis organic acids Pythium aphanidermatum compost
topic	misc QH301-705.5 misc antifungal activity misc antifungal proteins misc Aspergillus piperis misc organic acids misc Pythium aphanidermatum misc compost misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc antifungal activity misc antifungal proteins misc Aspergillus piperis misc organic acids misc Pythium aphanidermatum misc compost misc Biology (General)
topic_browse	misc QH301-705.5 misc antifungal activity misc antifungal proteins misc Aspergillus piperis misc organic acids misc Pythium aphanidermatum misc compost misc Biology (General)
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title	Aspergillus piperis A/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen Pythium aphanidermatum
ctrlnum	(DE-627)DOAJ011975105 (DE-599)DOAJe541db437c0644aba8b02f7142cd7a6e
title_full	Aspergillus piperis A/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen Pythium aphanidermatum
author_sort	Jovičić-Petrović Jelena
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author_browse	Jovičić-Petrović Jelena Jeremić Sanja Vučković Ivan Vojnović Sandra Bulajić Aleksandra Raičević Vera Nikodinovic-Runic Jasmina
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title_sort	aspergillus piperis a/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen pythium aphanidermatum
callnumber	QH301-705.5
title_auth	Aspergillus piperis A/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen Pythium aphanidermatum
abstract	Adding compost to soil can result in plant disease suppression through the mechanisms of antagonistic action of compost microflora against plant pathogens. The aim of the study was to select effective antagonists of Pythium aphanidermatum from compost, to assess the effect of its extracellular metabolites on the plant pathogen, and to characterize antifungal metabolites. The fungal isolate selected by a confrontation test was identified as Aspergillus piperis A/5 on the basis of morphological features and the internal transcribed spacer (ITS) region, β-tubulin and calmodulin partial sequences. Liquid chromatography-mass spectroscopy (LC-MS) analysis showed that gluconic and citric acid were the most abundant in the organic culture extract. However, the main antifungal activity was contained in the aqueous phase remaining after the organic solvent extraction. The presence of considerable amounts of proteins in both the crude culture extract as well as the aqueous phase remaining after solvent extraction was confirmed by SDS-PAGE. Isolated Aspergillus piperis A/5 exhibits strong antifungal activity against the phytopathogen Pythium aphanidermatum. It secretes a complex mixture of metabolites consisting of small molecules, including gluconic acid, citric acid and itaconic acid derivatives, but the most potent antifungal activity was associated with proteins resistant to heat and organic solvents. Our findings about the activity and characterization of antagonistic strain metabolites contribute to the understanding of the mechanism of interaction of antifungal metabolites as well as fungal-fungal interaction. The obtained results provide a basis for further application development in agriculture and food processing. [Projekat Ministarstva nauke Republike Srbije, br. TR31080 i br. ON173048, i EU Commission project AREA, br.316004]
abstractGer	Adding compost to soil can result in plant disease suppression through the mechanisms of antagonistic action of compost microflora against plant pathogens. The aim of the study was to select effective antagonists of Pythium aphanidermatum from compost, to assess the effect of its extracellular metabolites on the plant pathogen, and to characterize antifungal metabolites. The fungal isolate selected by a confrontation test was identified as Aspergillus piperis A/5 on the basis of morphological features and the internal transcribed spacer (ITS) region, β-tubulin and calmodulin partial sequences. Liquid chromatography-mass spectroscopy (LC-MS) analysis showed that gluconic and citric acid were the most abundant in the organic culture extract. However, the main antifungal activity was contained in the aqueous phase remaining after the organic solvent extraction. The presence of considerable amounts of proteins in both the crude culture extract as well as the aqueous phase remaining after solvent extraction was confirmed by SDS-PAGE. Isolated Aspergillus piperis A/5 exhibits strong antifungal activity against the phytopathogen Pythium aphanidermatum. It secretes a complex mixture of metabolites consisting of small molecules, including gluconic acid, citric acid and itaconic acid derivatives, but the most potent antifungal activity was associated with proteins resistant to heat and organic solvents. Our findings about the activity and characterization of antagonistic strain metabolites contribute to the understanding of the mechanism of interaction of antifungal metabolites as well as fungal-fungal interaction. The obtained results provide a basis for further application development in agriculture and food processing. [Projekat Ministarstva nauke Republike Srbije, br. TR31080 i br. ON173048, i EU Commission project AREA, br.316004]
abstract_unstemmed	Adding compost to soil can result in plant disease suppression through the mechanisms of antagonistic action of compost microflora against plant pathogens. The aim of the study was to select effective antagonists of Pythium aphanidermatum from compost, to assess the effect of its extracellular metabolites on the plant pathogen, and to characterize antifungal metabolites. The fungal isolate selected by a confrontation test was identified as Aspergillus piperis A/5 on the basis of morphological features and the internal transcribed spacer (ITS) region, β-tubulin and calmodulin partial sequences. Liquid chromatography-mass spectroscopy (LC-MS) analysis showed that gluconic and citric acid were the most abundant in the organic culture extract. However, the main antifungal activity was contained in the aqueous phase remaining after the organic solvent extraction. The presence of considerable amounts of proteins in both the crude culture extract as well as the aqueous phase remaining after solvent extraction was confirmed by SDS-PAGE. Isolated Aspergillus piperis A/5 exhibits strong antifungal activity against the phytopathogen Pythium aphanidermatum. It secretes a complex mixture of metabolites consisting of small molecules, including gluconic acid, citric acid and itaconic acid derivatives, but the most potent antifungal activity was associated with proteins resistant to heat and organic solvents. Our findings about the activity and characterization of antagonistic strain metabolites contribute to the understanding of the mechanism of interaction of antifungal metabolites as well as fungal-fungal interaction. The obtained results provide a basis for further application development in agriculture and food processing. [Projekat Ministarstva nauke Republike Srbije, br. TR31080 i br. ON173048, i EU Commission project AREA, br.316004]
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title_short	Aspergillus piperis A/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen Pythium aphanidermatum
url	https://doi.org/10.2298/ABS150602016J https://doaj.org/article/e541db437c0644aba8b02f7142cd7a6e http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600016J.pdf https://doaj.org/toc/0354-4664 https://doaj.org/toc/1821-4339
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author2	Jeremić Sanja Vučković Ivan Vojnović Sandra Bulajić Aleksandra Raičević Vera Nikodinovic-Runic Jasmina
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up_date	2024-07-03T23:09:37.544Z
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