Expression level of the DREB2-type gene, identified with Amplifluor SNP markers, correlates with performance and tolerance to dehydration in bread wheat cultivars from Northern Kazakhstan

A panel of 89 local commercial cultivars of bread wheat was tested in field trials in the dry conditions of Northern Kazakhstan. Two distinct groups of cultivars (six cultivars in each group), which had the highest and the lowest grain yield under drought were selected for further experiments. A deh...
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Autor*in:	Yuri Shavrukov [verfasserIn] Aibek Zhumalin [verfasserIn] Dauren Serikbay [verfasserIn] Makpal Botayeva [verfasserIn] Ainur Otemisova [verfasserIn] Aiman Absattarova [verfasserIn] Grigoriy Sereda [verfasserIn] Sergey Sereda [verfasserIn] Vladimir Shvidchenko [verfasserIn] Arysgul Turbekova [verfasserIn] Satyvaldy Jatayev [verfasserIn] Sergiy Lopato [verfasserIn] Kathleen Soole [verfasserIn] Peter Langridge [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	Dehydration Gene Expression genotyping bread wheat grain yield DREB2

Übergeordnetes Werk:	In: Frontiers in Plant Science - Frontiers Media S.A., 2011, 7(2016)
Übergeordnetes Werk:	volume:7 ; year:2016

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fpls.2016.01736

Katalog-ID:	DOAJ012103721

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520			\|a A panel of 89 local commercial cultivars of bread wheat was tested in field trials in the dry conditions of Northern Kazakhstan. Two distinct groups of cultivars (six cultivars in each group), which had the highest and the lowest grain yield under drought were selected for further experiments. A dehydration test conducted on detached leaves indicated a strong association between rates of water loss in plants from the first group with highest grain yield production in the dry environment relative to the second group. Modern high-throughput Amplifluor SNP technology was applied to study allelic variations in a series of drought-responsive genes using 19 SNP markers. Genotyping of an SNP in the TaDREB5 (DREB2-type) gene using the Amplifluor SNP marker KATU48 revealed clear allele distribution across the entire panel of wheat accessions, and distinguished between the two groups of cultivars with high and low yield under drought. Significant differences in expression levels of TaDREB5 were revealed by qRT-PCR. Most wheat plants from the first group of cultivars with high grain yield showed strong up-regulation of TaDREB5 transcript in dehydrated leaves. In contrast, expression of TaDREB5 in plants from the second group of cultivars with low grain yield was significantly down-regulated. It was found that SNPs did not alter the amino acid sequence of TaDREB5 protein. Thus, a possible explanation is that alternative splicing and up-stream regulation of TaDREB5 may be affected by SNP, but these hypotheses require additional analysis (and will be the focus of future studies).
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allfields	10.3389/fpls.2016.01736 doi (DE-627)DOAJ012103721 (DE-599)DOAJ5627869c60eb4c269c724708f3201f67 DE-627 ger DE-627 rakwb eng SB1-1110 Yuri Shavrukov verfasserin aut Expression level of the DREB2-type gene, identified with Amplifluor SNP markers, correlates with performance and tolerance to dehydration in bread wheat cultivars from Northern Kazakhstan 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A panel of 89 local commercial cultivars of bread wheat was tested in field trials in the dry conditions of Northern Kazakhstan. Two distinct groups of cultivars (six cultivars in each group), which had the highest and the lowest grain yield under drought were selected for further experiments. A dehydration test conducted on detached leaves indicated a strong association between rates of water loss in plants from the first group with highest grain yield production in the dry environment relative to the second group. Modern high-throughput Amplifluor SNP technology was applied to study allelic variations in a series of drought-responsive genes using 19 SNP markers. Genotyping of an SNP in the TaDREB5 (DREB2-type) gene using the Amplifluor SNP marker KATU48 revealed clear allele distribution across the entire panel of wheat accessions, and distinguished between the two groups of cultivars with high and low yield under drought. Significant differences in expression levels of TaDREB5 were revealed by qRT-PCR. Most wheat plants from the first group of cultivars with high grain yield showed strong up-regulation of TaDREB5 transcript in dehydrated leaves. In contrast, expression of TaDREB5 in plants from the second group of cultivars with low grain yield was significantly down-regulated. It was found that SNPs did not alter the amino acid sequence of TaDREB5 protein. Thus, a possible explanation is that alternative splicing and up-stream regulation of TaDREB5 may be affected by SNP, but these hypotheses require additional analysis (and will be the focus of future studies). Dehydration Gene Expression genotyping bread wheat grain yield DREB2 Plant culture Yuri Shavrukov verfasserin aut Aibek Zhumalin verfasserin aut Dauren Serikbay verfasserin aut Makpal Botayeva verfasserin aut Ainur Otemisova verfasserin aut Aiman Absattarova verfasserin aut Grigoriy Sereda verfasserin aut Sergey Sereda verfasserin aut Vladimir Shvidchenko verfasserin aut Arysgul Turbekova verfasserin aut Satyvaldy Jatayev verfasserin aut Sergiy Lopato verfasserin aut Kathleen Soole verfasserin aut Peter Langridge verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 7(2016) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:7 year:2016 https://doi.org/10.3389/fpls.2016.01736 kostenfrei https://doaj.org/article/5627869c60eb4c269c724708f3201f67 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01736/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2016
spelling	10.3389/fpls.2016.01736 doi (DE-627)DOAJ012103721 (DE-599)DOAJ5627869c60eb4c269c724708f3201f67 DE-627 ger DE-627 rakwb eng SB1-1110 Yuri Shavrukov verfasserin aut Expression level of the DREB2-type gene, identified with Amplifluor SNP markers, correlates with performance and tolerance to dehydration in bread wheat cultivars from Northern Kazakhstan 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A panel of 89 local commercial cultivars of bread wheat was tested in field trials in the dry conditions of Northern Kazakhstan. Two distinct groups of cultivars (six cultivars in each group), which had the highest and the lowest grain yield under drought were selected for further experiments. A dehydration test conducted on detached leaves indicated a strong association between rates of water loss in plants from the first group with highest grain yield production in the dry environment relative to the second group. Modern high-throughput Amplifluor SNP technology was applied to study allelic variations in a series of drought-responsive genes using 19 SNP markers. Genotyping of an SNP in the TaDREB5 (DREB2-type) gene using the Amplifluor SNP marker KATU48 revealed clear allele distribution across the entire panel of wheat accessions, and distinguished between the two groups of cultivars with high and low yield under drought. Significant differences in expression levels of TaDREB5 were revealed by qRT-PCR. Most wheat plants from the first group of cultivars with high grain yield showed strong up-regulation of TaDREB5 transcript in dehydrated leaves. In contrast, expression of TaDREB5 in plants from the second group of cultivars with low grain yield was significantly down-regulated. It was found that SNPs did not alter the amino acid sequence of TaDREB5 protein. Thus, a possible explanation is that alternative splicing and up-stream regulation of TaDREB5 may be affected by SNP, but these hypotheses require additional analysis (and will be the focus of future studies). Dehydration Gene Expression genotyping bread wheat grain yield DREB2 Plant culture Yuri Shavrukov verfasserin aut Aibek Zhumalin verfasserin aut Dauren Serikbay verfasserin aut Makpal Botayeva verfasserin aut Ainur Otemisova verfasserin aut Aiman Absattarova verfasserin aut Grigoriy Sereda verfasserin aut Sergey Sereda verfasserin aut Vladimir Shvidchenko verfasserin aut Arysgul Turbekova verfasserin aut Satyvaldy Jatayev verfasserin aut Sergiy Lopato verfasserin aut Kathleen Soole verfasserin aut Peter Langridge verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 7(2016) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:7 year:2016 https://doi.org/10.3389/fpls.2016.01736 kostenfrei https://doaj.org/article/5627869c60eb4c269c724708f3201f67 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01736/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2016
allfields_unstemmed	10.3389/fpls.2016.01736 doi (DE-627)DOAJ012103721 (DE-599)DOAJ5627869c60eb4c269c724708f3201f67 DE-627 ger DE-627 rakwb eng SB1-1110 Yuri Shavrukov verfasserin aut Expression level of the DREB2-type gene, identified with Amplifluor SNP markers, correlates with performance and tolerance to dehydration in bread wheat cultivars from Northern Kazakhstan 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A panel of 89 local commercial cultivars of bread wheat was tested in field trials in the dry conditions of Northern Kazakhstan. Two distinct groups of cultivars (six cultivars in each group), which had the highest and the lowest grain yield under drought were selected for further experiments. A dehydration test conducted on detached leaves indicated a strong association between rates of water loss in plants from the first group with highest grain yield production in the dry environment relative to the second group. Modern high-throughput Amplifluor SNP technology was applied to study allelic variations in a series of drought-responsive genes using 19 SNP markers. Genotyping of an SNP in the TaDREB5 (DREB2-type) gene using the Amplifluor SNP marker KATU48 revealed clear allele distribution across the entire panel of wheat accessions, and distinguished between the two groups of cultivars with high and low yield under drought. Significant differences in expression levels of TaDREB5 were revealed by qRT-PCR. Most wheat plants from the first group of cultivars with high grain yield showed strong up-regulation of TaDREB5 transcript in dehydrated leaves. In contrast, expression of TaDREB5 in plants from the second group of cultivars with low grain yield was significantly down-regulated. It was found that SNPs did not alter the amino acid sequence of TaDREB5 protein. Thus, a possible explanation is that alternative splicing and up-stream regulation of TaDREB5 may be affected by SNP, but these hypotheses require additional analysis (and will be the focus of future studies). Dehydration Gene Expression genotyping bread wheat grain yield DREB2 Plant culture Yuri Shavrukov verfasserin aut Aibek Zhumalin verfasserin aut Dauren Serikbay verfasserin aut Makpal Botayeva verfasserin aut Ainur Otemisova verfasserin aut Aiman Absattarova verfasserin aut Grigoriy Sereda verfasserin aut Sergey Sereda verfasserin aut Vladimir Shvidchenko verfasserin aut Arysgul Turbekova verfasserin aut Satyvaldy Jatayev verfasserin aut Sergiy Lopato verfasserin aut Kathleen Soole verfasserin aut Peter Langridge verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 7(2016) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:7 year:2016 https://doi.org/10.3389/fpls.2016.01736 kostenfrei https://doaj.org/article/5627869c60eb4c269c724708f3201f67 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01736/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2016
allfieldsGer	10.3389/fpls.2016.01736 doi (DE-627)DOAJ012103721 (DE-599)DOAJ5627869c60eb4c269c724708f3201f67 DE-627 ger DE-627 rakwb eng SB1-1110 Yuri Shavrukov verfasserin aut Expression level of the DREB2-type gene, identified with Amplifluor SNP markers, correlates with performance and tolerance to dehydration in bread wheat cultivars from Northern Kazakhstan 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A panel of 89 local commercial cultivars of bread wheat was tested in field trials in the dry conditions of Northern Kazakhstan. Two distinct groups of cultivars (six cultivars in each group), which had the highest and the lowest grain yield under drought were selected for further experiments. A dehydration test conducted on detached leaves indicated a strong association between rates of water loss in plants from the first group with highest grain yield production in the dry environment relative to the second group. Modern high-throughput Amplifluor SNP technology was applied to study allelic variations in a series of drought-responsive genes using 19 SNP markers. Genotyping of an SNP in the TaDREB5 (DREB2-type) gene using the Amplifluor SNP marker KATU48 revealed clear allele distribution across the entire panel of wheat accessions, and distinguished between the two groups of cultivars with high and low yield under drought. Significant differences in expression levels of TaDREB5 were revealed by qRT-PCR. Most wheat plants from the first group of cultivars with high grain yield showed strong up-regulation of TaDREB5 transcript in dehydrated leaves. In contrast, expression of TaDREB5 in plants from the second group of cultivars with low grain yield was significantly down-regulated. It was found that SNPs did not alter the amino acid sequence of TaDREB5 protein. Thus, a possible explanation is that alternative splicing and up-stream regulation of TaDREB5 may be affected by SNP, but these hypotheses require additional analysis (and will be the focus of future studies). Dehydration Gene Expression genotyping bread wheat grain yield DREB2 Plant culture Yuri Shavrukov verfasserin aut Aibek Zhumalin verfasserin aut Dauren Serikbay verfasserin aut Makpal Botayeva verfasserin aut Ainur Otemisova verfasserin aut Aiman Absattarova verfasserin aut Grigoriy Sereda verfasserin aut Sergey Sereda verfasserin aut Vladimir Shvidchenko verfasserin aut Arysgul Turbekova verfasserin aut Satyvaldy Jatayev verfasserin aut Sergiy Lopato verfasserin aut Kathleen Soole verfasserin aut Peter Langridge verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 7(2016) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:7 year:2016 https://doi.org/10.3389/fpls.2016.01736 kostenfrei https://doaj.org/article/5627869c60eb4c269c724708f3201f67 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01736/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2016
allfieldsSound	10.3389/fpls.2016.01736 doi (DE-627)DOAJ012103721 (DE-599)DOAJ5627869c60eb4c269c724708f3201f67 DE-627 ger DE-627 rakwb eng SB1-1110 Yuri Shavrukov verfasserin aut Expression level of the DREB2-type gene, identified with Amplifluor SNP markers, correlates with performance and tolerance to dehydration in bread wheat cultivars from Northern Kazakhstan 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A panel of 89 local commercial cultivars of bread wheat was tested in field trials in the dry conditions of Northern Kazakhstan. Two distinct groups of cultivars (six cultivars in each group), which had the highest and the lowest grain yield under drought were selected for further experiments. A dehydration test conducted on detached leaves indicated a strong association between rates of water loss in plants from the first group with highest grain yield production in the dry environment relative to the second group. Modern high-throughput Amplifluor SNP technology was applied to study allelic variations in a series of drought-responsive genes using 19 SNP markers. Genotyping of an SNP in the TaDREB5 (DREB2-type) gene using the Amplifluor SNP marker KATU48 revealed clear allele distribution across the entire panel of wheat accessions, and distinguished between the two groups of cultivars with high and low yield under drought. Significant differences in expression levels of TaDREB5 were revealed by qRT-PCR. Most wheat plants from the first group of cultivars with high grain yield showed strong up-regulation of TaDREB5 transcript in dehydrated leaves. In contrast, expression of TaDREB5 in plants from the second group of cultivars with low grain yield was significantly down-regulated. It was found that SNPs did not alter the amino acid sequence of TaDREB5 protein. Thus, a possible explanation is that alternative splicing and up-stream regulation of TaDREB5 may be affected by SNP, but these hypotheses require additional analysis (and will be the focus of future studies). Dehydration Gene Expression genotyping bread wheat grain yield DREB2 Plant culture Yuri Shavrukov verfasserin aut Aibek Zhumalin verfasserin aut Dauren Serikbay verfasserin aut Makpal Botayeva verfasserin aut Ainur Otemisova verfasserin aut Aiman Absattarova verfasserin aut Grigoriy Sereda verfasserin aut Sergey Sereda verfasserin aut Vladimir Shvidchenko verfasserin aut Arysgul Turbekova verfasserin aut Satyvaldy Jatayev verfasserin aut Sergiy Lopato verfasserin aut Kathleen Soole verfasserin aut Peter Langridge verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 7(2016) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:7 year:2016 https://doi.org/10.3389/fpls.2016.01736 kostenfrei https://doaj.org/article/5627869c60eb4c269c724708f3201f67 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01736/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2016
language	English
source	In Frontiers in Plant Science 7(2016) volume:7 year:2016
sourceStr	In Frontiers in Plant Science 7(2016) volume:7 year:2016
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Dehydration Gene Expression genotyping bread wheat grain yield DREB2 Plant culture
isfreeaccess_bool	true
container_title	Frontiers in Plant Science
authorswithroles_txt_mv	Yuri Shavrukov @@aut@@ Aibek Zhumalin @@aut@@ Dauren Serikbay @@aut@@ Makpal Botayeva @@aut@@ Ainur Otemisova @@aut@@ Aiman Absattarova @@aut@@ Grigoriy Sereda @@aut@@ Sergey Sereda @@aut@@ Vladimir Shvidchenko @@aut@@ Arysgul Turbekova @@aut@@ Satyvaldy Jatayev @@aut@@ Sergiy Lopato @@aut@@ Kathleen Soole @@aut@@ Peter Langridge @@aut@@
publishDateDaySort_date	2016-01-01T00:00:00Z
hierarchy_top_id	662359240
id	DOAJ012103721
language_de	englisch
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callnumber-first	S - Agriculture
author	Yuri Shavrukov
spellingShingle	Yuri Shavrukov misc SB1-1110 misc Dehydration misc Gene Expression misc genotyping misc bread wheat misc grain yield misc DREB2 misc Plant culture Expression level of the DREB2-type gene, identified with Amplifluor SNP markers, correlates with performance and tolerance to dehydration in bread wheat cultivars from Northern Kazakhstan
authorStr	Yuri Shavrukov
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topic_title	SB1-1110 Expression level of the DREB2-type gene, identified with Amplifluor SNP markers, correlates with performance and tolerance to dehydration in bread wheat cultivars from Northern Kazakhstan Dehydration Gene Expression genotyping bread wheat grain yield DREB2
topic	misc SB1-1110 misc Dehydration misc Gene Expression misc genotyping misc bread wheat misc grain yield misc DREB2 misc Plant culture
topic_unstemmed	misc SB1-1110 misc Dehydration misc Gene Expression misc genotyping misc bread wheat misc grain yield misc DREB2 misc Plant culture
topic_browse	misc SB1-1110 misc Dehydration misc Gene Expression misc genotyping misc bread wheat misc grain yield misc DREB2 misc Plant culture
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title	Expression level of the DREB2-type gene, identified with Amplifluor SNP markers, correlates with performance and tolerance to dehydration in bread wheat cultivars from Northern Kazakhstan
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title_full	Expression level of the DREB2-type gene, identified with Amplifluor SNP markers, correlates with performance and tolerance to dehydration in bread wheat cultivars from Northern Kazakhstan
author_sort	Yuri Shavrukov
journal	Frontiers in Plant Science
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author_browse	Yuri Shavrukov Aibek Zhumalin Dauren Serikbay Makpal Botayeva Ainur Otemisova Aiman Absattarova Grigoriy Sereda Sergey Sereda Vladimir Shvidchenko Arysgul Turbekova Satyvaldy Jatayev Sergiy Lopato Kathleen Soole Peter Langridge
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title_sort	expression level of the dreb2-type gene, identified with amplifluor snp markers, correlates with performance and tolerance to dehydration in bread wheat cultivars from northern kazakhstan
callnumber	SB1-1110
title_auth	Expression level of the DREB2-type gene, identified with Amplifluor SNP markers, correlates with performance and tolerance to dehydration in bread wheat cultivars from Northern Kazakhstan
abstract	A panel of 89 local commercial cultivars of bread wheat was tested in field trials in the dry conditions of Northern Kazakhstan. Two distinct groups of cultivars (six cultivars in each group), which had the highest and the lowest grain yield under drought were selected for further experiments. A dehydration test conducted on detached leaves indicated a strong association between rates of water loss in plants from the first group with highest grain yield production in the dry environment relative to the second group. Modern high-throughput Amplifluor SNP technology was applied to study allelic variations in a series of drought-responsive genes using 19 SNP markers. Genotyping of an SNP in the TaDREB5 (DREB2-type) gene using the Amplifluor SNP marker KATU48 revealed clear allele distribution across the entire panel of wheat accessions, and distinguished between the two groups of cultivars with high and low yield under drought. Significant differences in expression levels of TaDREB5 were revealed by qRT-PCR. Most wheat plants from the first group of cultivars with high grain yield showed strong up-regulation of TaDREB5 transcript in dehydrated leaves. In contrast, expression of TaDREB5 in plants from the second group of cultivars with low grain yield was significantly down-regulated. It was found that SNPs did not alter the amino acid sequence of TaDREB5 protein. Thus, a possible explanation is that alternative splicing and up-stream regulation of TaDREB5 may be affected by SNP, but these hypotheses require additional analysis (and will be the focus of future studies).
abstractGer	A panel of 89 local commercial cultivars of bread wheat was tested in field trials in the dry conditions of Northern Kazakhstan. Two distinct groups of cultivars (six cultivars in each group), which had the highest and the lowest grain yield under drought were selected for further experiments. A dehydration test conducted on detached leaves indicated a strong association between rates of water loss in plants from the first group with highest grain yield production in the dry environment relative to the second group. Modern high-throughput Amplifluor SNP technology was applied to study allelic variations in a series of drought-responsive genes using 19 SNP markers. Genotyping of an SNP in the TaDREB5 (DREB2-type) gene using the Amplifluor SNP marker KATU48 revealed clear allele distribution across the entire panel of wheat accessions, and distinguished between the two groups of cultivars with high and low yield under drought. Significant differences in expression levels of TaDREB5 were revealed by qRT-PCR. Most wheat plants from the first group of cultivars with high grain yield showed strong up-regulation of TaDREB5 transcript in dehydrated leaves. In contrast, expression of TaDREB5 in plants from the second group of cultivars with low grain yield was significantly down-regulated. It was found that SNPs did not alter the amino acid sequence of TaDREB5 protein. Thus, a possible explanation is that alternative splicing and up-stream regulation of TaDREB5 may be affected by SNP, but these hypotheses require additional analysis (and will be the focus of future studies).
abstract_unstemmed	A panel of 89 local commercial cultivars of bread wheat was tested in field trials in the dry conditions of Northern Kazakhstan. Two distinct groups of cultivars (six cultivars in each group), which had the highest and the lowest grain yield under drought were selected for further experiments. A dehydration test conducted on detached leaves indicated a strong association between rates of water loss in plants from the first group with highest grain yield production in the dry environment relative to the second group. Modern high-throughput Amplifluor SNP technology was applied to study allelic variations in a series of drought-responsive genes using 19 SNP markers. Genotyping of an SNP in the TaDREB5 (DREB2-type) gene using the Amplifluor SNP marker KATU48 revealed clear allele distribution across the entire panel of wheat accessions, and distinguished between the two groups of cultivars with high and low yield under drought. Significant differences in expression levels of TaDREB5 were revealed by qRT-PCR. Most wheat plants from the first group of cultivars with high grain yield showed strong up-regulation of TaDREB5 transcript in dehydrated leaves. In contrast, expression of TaDREB5 in plants from the second group of cultivars with low grain yield was significantly down-regulated. It was found that SNPs did not alter the amino acid sequence of TaDREB5 protein. Thus, a possible explanation is that alternative splicing and up-stream regulation of TaDREB5 may be affected by SNP, but these hypotheses require additional analysis (and will be the focus of future studies).
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title_short	Expression level of the DREB2-type gene, identified with Amplifluor SNP markers, correlates with performance and tolerance to dehydration in bread wheat cultivars from Northern Kazakhstan
url	https://doi.org/10.3389/fpls.2016.01736 https://doaj.org/article/5627869c60eb4c269c724708f3201f67 http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01736/full https://doaj.org/toc/1664-462X
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Expression level of the DREB2-type gene, identified with Amplifluor SNP markers, correlates with performance and tolerance to dehydration in bread wheat cultivars from Northern Kazakhstan

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