Role of the pre-neck appendage protein (Dpo7) from phage vB_SepiS-phiIPLA7 as an anti-biofilm agent in staphylococcal species

Staphylococcus epidermidis and Staphylococcus aureus are important causative agents of hospital-acquired infections and bacteremia, likely due to their ability to form biofilms. The production of a dense exopolysaccharide (EPS) matrix enclosing the cells slows the penetration of antibiotic down, res...
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Gespeichert in:

Autor*in:	Diana eGutiérrez [verfasserIn] Yves eBriers [verfasserIn] Lorena eRodríguez-Rubio [verfasserIn] Beatriz eMartínez [verfasserIn] Ana eRodríguez [verfasserIn] Rob eLavigne [verfasserIn] Pilar eGarcía [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	Biofilm S. aureus S. epidermidis Biofilm matrix Exopolysaccharide depolymerase

Übergeordnetes Werk:	In: Frontiers in Microbiology - Frontiers Media S.A., 2011, 6(2015)
Übergeordnetes Werk:	volume:6 ; year:2015

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fmicb.2015.01315

Katalog-ID:	DOAJ012241040

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allfields	10.3389/fmicb.2015.01315 doi (DE-627)DOAJ012241040 (DE-599)DOAJc2771b23ef0d4252a98801c4cfef9260 DE-627 ger DE-627 rakwb eng QR1-502 Diana eGutiérrez verfasserin aut Role of the pre-neck appendage protein (Dpo7) from phage vB_SepiS-phiIPLA7 as an anti-biofilm agent in staphylococcal species 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Staphylococcus epidermidis and Staphylococcus aureus are important causative agents of hospital-acquired infections and bacteremia, likely due to their ability to form biofilms. The production of a dense exopolysaccharide (EPS) matrix enclosing the cells slows the penetration of antibiotic down, resulting in therapy failure. The exopolysaccharide depolymerase (Dpo7) derived from bacteriophage vB_SepiS-phiIPLA7, was overexpressed in E. coli and characterized. A dose dependent but time independent response was observed after treatment of staphylococcal 24 h-biofilms with Dpo7. Maximum removal (<90%) of biofilm-attached cells was obtained with 0.15 µM of Dpo7 in all polysaccharide producer strains but Dpo7 failed to eliminate polysaccharide-independent biofilm formed by S. aureus V329. Moreover, the pre-treatment of polystyrene surfaces with Dpo7 reduced the biofilm biomass by 53-85% in the 67% of the tested strains. This study supports the use of phage-encoded exopolysaccharide depolymerases to prevent and disperse staphylococcal biofilms, thereby making bacteria more susceptible to the action of antimicrobials. Biofilm S. aureus S. epidermidis Biofilm matrix Exopolysaccharide depolymerase Microbiology Yves eBriers verfasserin aut Lorena eRodríguez-Rubio verfasserin aut Lorena eRodríguez-Rubio verfasserin aut Beatriz eMartínez verfasserin aut Ana eRodríguez verfasserin aut Rob eLavigne verfasserin aut Pilar eGarcía verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 6(2015) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:6 year:2015 https://doi.org/10.3389/fmicb.2015.01315 kostenfrei https://doaj.org/article/c2771b23ef0d4252a98801c4cfef9260 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01315/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
spelling	10.3389/fmicb.2015.01315 doi (DE-627)DOAJ012241040 (DE-599)DOAJc2771b23ef0d4252a98801c4cfef9260 DE-627 ger DE-627 rakwb eng QR1-502 Diana eGutiérrez verfasserin aut Role of the pre-neck appendage protein (Dpo7) from phage vB_SepiS-phiIPLA7 as an anti-biofilm agent in staphylococcal species 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Staphylococcus epidermidis and Staphylococcus aureus are important causative agents of hospital-acquired infections and bacteremia, likely due to their ability to form biofilms. The production of a dense exopolysaccharide (EPS) matrix enclosing the cells slows the penetration of antibiotic down, resulting in therapy failure. The exopolysaccharide depolymerase (Dpo7) derived from bacteriophage vB_SepiS-phiIPLA7, was overexpressed in E. coli and characterized. A dose dependent but time independent response was observed after treatment of staphylococcal 24 h-biofilms with Dpo7. Maximum removal (<90%) of biofilm-attached cells was obtained with 0.15 µM of Dpo7 in all polysaccharide producer strains but Dpo7 failed to eliminate polysaccharide-independent biofilm formed by S. aureus V329. Moreover, the pre-treatment of polystyrene surfaces with Dpo7 reduced the biofilm biomass by 53-85% in the 67% of the tested strains. This study supports the use of phage-encoded exopolysaccharide depolymerases to prevent and disperse staphylococcal biofilms, thereby making bacteria more susceptible to the action of antimicrobials. Biofilm S. aureus S. epidermidis Biofilm matrix Exopolysaccharide depolymerase Microbiology Yves eBriers verfasserin aut Lorena eRodríguez-Rubio verfasserin aut Lorena eRodríguez-Rubio verfasserin aut Beatriz eMartínez verfasserin aut Ana eRodríguez verfasserin aut Rob eLavigne verfasserin aut Pilar eGarcía verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 6(2015) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:6 year:2015 https://doi.org/10.3389/fmicb.2015.01315 kostenfrei https://doaj.org/article/c2771b23ef0d4252a98801c4cfef9260 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01315/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
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allfieldsGer	10.3389/fmicb.2015.01315 doi (DE-627)DOAJ012241040 (DE-599)DOAJc2771b23ef0d4252a98801c4cfef9260 DE-627 ger DE-627 rakwb eng QR1-502 Diana eGutiérrez verfasserin aut Role of the pre-neck appendage protein (Dpo7) from phage vB_SepiS-phiIPLA7 as an anti-biofilm agent in staphylococcal species 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Staphylococcus epidermidis and Staphylococcus aureus are important causative agents of hospital-acquired infections and bacteremia, likely due to their ability to form biofilms. The production of a dense exopolysaccharide (EPS) matrix enclosing the cells slows the penetration of antibiotic down, resulting in therapy failure. The exopolysaccharide depolymerase (Dpo7) derived from bacteriophage vB_SepiS-phiIPLA7, was overexpressed in E. coli and characterized. A dose dependent but time independent response was observed after treatment of staphylococcal 24 h-biofilms with Dpo7. Maximum removal (<90%) of biofilm-attached cells was obtained with 0.15 µM of Dpo7 in all polysaccharide producer strains but Dpo7 failed to eliminate polysaccharide-independent biofilm formed by S. aureus V329. Moreover, the pre-treatment of polystyrene surfaces with Dpo7 reduced the biofilm biomass by 53-85% in the 67% of the tested strains. This study supports the use of phage-encoded exopolysaccharide depolymerases to prevent and disperse staphylococcal biofilms, thereby making bacteria more susceptible to the action of antimicrobials. Biofilm S. aureus S. epidermidis Biofilm matrix Exopolysaccharide depolymerase Microbiology Yves eBriers verfasserin aut Lorena eRodríguez-Rubio verfasserin aut Lorena eRodríguez-Rubio verfasserin aut Beatriz eMartínez verfasserin aut Ana eRodríguez verfasserin aut Rob eLavigne verfasserin aut Pilar eGarcía verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 6(2015) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:6 year:2015 https://doi.org/10.3389/fmicb.2015.01315 kostenfrei https://doaj.org/article/c2771b23ef0d4252a98801c4cfef9260 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01315/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
allfieldsSound	10.3389/fmicb.2015.01315 doi (DE-627)DOAJ012241040 (DE-599)DOAJc2771b23ef0d4252a98801c4cfef9260 DE-627 ger DE-627 rakwb eng QR1-502 Diana eGutiérrez verfasserin aut Role of the pre-neck appendage protein (Dpo7) from phage vB_SepiS-phiIPLA7 as an anti-biofilm agent in staphylococcal species 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Staphylococcus epidermidis and Staphylococcus aureus are important causative agents of hospital-acquired infections and bacteremia, likely due to their ability to form biofilms. The production of a dense exopolysaccharide (EPS) matrix enclosing the cells slows the penetration of antibiotic down, resulting in therapy failure. The exopolysaccharide depolymerase (Dpo7) derived from bacteriophage vB_SepiS-phiIPLA7, was overexpressed in E. coli and characterized. A dose dependent but time independent response was observed after treatment of staphylococcal 24 h-biofilms with Dpo7. Maximum removal (<90%) of biofilm-attached cells was obtained with 0.15 µM of Dpo7 in all polysaccharide producer strains but Dpo7 failed to eliminate polysaccharide-independent biofilm formed by S. aureus V329. Moreover, the pre-treatment of polystyrene surfaces with Dpo7 reduced the biofilm biomass by 53-85% in the 67% of the tested strains. This study supports the use of phage-encoded exopolysaccharide depolymerases to prevent and disperse staphylococcal biofilms, thereby making bacteria more susceptible to the action of antimicrobials. Biofilm S. aureus S. epidermidis Biofilm matrix Exopolysaccharide depolymerase Microbiology Yves eBriers verfasserin aut Lorena eRodríguez-Rubio verfasserin aut Lorena eRodríguez-Rubio verfasserin aut Beatriz eMartínez verfasserin aut Ana eRodríguez verfasserin aut Rob eLavigne verfasserin aut Pilar eGarcía verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 6(2015) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:6 year:2015 https://doi.org/10.3389/fmicb.2015.01315 kostenfrei https://doaj.org/article/c2771b23ef0d4252a98801c4cfef9260 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01315/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
language	English
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callnumber-first	Q - Science
author	Diana eGutiérrez
spellingShingle	Diana eGutiérrez misc QR1-502 misc Biofilm misc S. aureus misc S. epidermidis misc Biofilm matrix misc Exopolysaccharide depolymerase misc Microbiology Role of the pre-neck appendage protein (Dpo7) from phage vB_SepiS-phiIPLA7 as an anti-biofilm agent in staphylococcal species
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topic_title	QR1-502 Role of the pre-neck appendage protein (Dpo7) from phage vB_SepiS-phiIPLA7 as an anti-biofilm agent in staphylococcal species Biofilm S. aureus S. epidermidis Biofilm matrix Exopolysaccharide depolymerase
topic	misc QR1-502 misc Biofilm misc S. aureus misc S. epidermidis misc Biofilm matrix misc Exopolysaccharide depolymerase misc Microbiology
topic_unstemmed	misc QR1-502 misc Biofilm misc S. aureus misc S. epidermidis misc Biofilm matrix misc Exopolysaccharide depolymerase misc Microbiology
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title	Role of the pre-neck appendage protein (Dpo7) from phage vB_SepiS-phiIPLA7 as an anti-biofilm agent in staphylococcal species
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title_full	Role of the pre-neck appendage protein (Dpo7) from phage vB_SepiS-phiIPLA7 as an anti-biofilm agent in staphylococcal species
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title_sort	role of the pre-neck appendage protein (dpo7) from phage vb_sepis-phiipla7 as an anti-biofilm agent in staphylococcal species
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title_auth	Role of the pre-neck appendage protein (Dpo7) from phage vB_SepiS-phiIPLA7 as an anti-biofilm agent in staphylococcal species
abstract	Staphylococcus epidermidis and Staphylococcus aureus are important causative agents of hospital-acquired infections and bacteremia, likely due to their ability to form biofilms. The production of a dense exopolysaccharide (EPS) matrix enclosing the cells slows the penetration of antibiotic down, resulting in therapy failure. The exopolysaccharide depolymerase (Dpo7) derived from bacteriophage vB_SepiS-phiIPLA7, was overexpressed in E. coli and characterized. A dose dependent but time independent response was observed after treatment of staphylococcal 24 h-biofilms with Dpo7. Maximum removal (<90%) of biofilm-attached cells was obtained with 0.15 µM of Dpo7 in all polysaccharide producer strains but Dpo7 failed to eliminate polysaccharide-independent biofilm formed by S. aureus V329. Moreover, the pre-treatment of polystyrene surfaces with Dpo7 reduced the biofilm biomass by 53-85% in the 67% of the tested strains. This study supports the use of phage-encoded exopolysaccharide depolymerases to prevent and disperse staphylococcal biofilms, thereby making bacteria more susceptible to the action of antimicrobials.
abstractGer	Staphylococcus epidermidis and Staphylococcus aureus are important causative agents of hospital-acquired infections and bacteremia, likely due to their ability to form biofilms. The production of a dense exopolysaccharide (EPS) matrix enclosing the cells slows the penetration of antibiotic down, resulting in therapy failure. The exopolysaccharide depolymerase (Dpo7) derived from bacteriophage vB_SepiS-phiIPLA7, was overexpressed in E. coli and characterized. A dose dependent but time independent response was observed after treatment of staphylococcal 24 h-biofilms with Dpo7. Maximum removal (<90%) of biofilm-attached cells was obtained with 0.15 µM of Dpo7 in all polysaccharide producer strains but Dpo7 failed to eliminate polysaccharide-independent biofilm formed by S. aureus V329. Moreover, the pre-treatment of polystyrene surfaces with Dpo7 reduced the biofilm biomass by 53-85% in the 67% of the tested strains. This study supports the use of phage-encoded exopolysaccharide depolymerases to prevent and disperse staphylococcal biofilms, thereby making bacteria more susceptible to the action of antimicrobials.
abstract_unstemmed	Staphylococcus epidermidis and Staphylococcus aureus are important causative agents of hospital-acquired infections and bacteremia, likely due to their ability to form biofilms. The production of a dense exopolysaccharide (EPS) matrix enclosing the cells slows the penetration of antibiotic down, resulting in therapy failure. The exopolysaccharide depolymerase (Dpo7) derived from bacteriophage vB_SepiS-phiIPLA7, was overexpressed in E. coli and characterized. A dose dependent but time independent response was observed after treatment of staphylococcal 24 h-biofilms with Dpo7. Maximum removal (<90%) of biofilm-attached cells was obtained with 0.15 µM of Dpo7 in all polysaccharide producer strains but Dpo7 failed to eliminate polysaccharide-independent biofilm formed by S. aureus V329. Moreover, the pre-treatment of polystyrene surfaces with Dpo7 reduced the biofilm biomass by 53-85% in the 67% of the tested strains. This study supports the use of phage-encoded exopolysaccharide depolymerases to prevent and disperse staphylococcal biofilms, thereby making bacteria more susceptible to the action of antimicrobials.
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title_short	Role of the pre-neck appendage protein (Dpo7) from phage vB_SepiS-phiIPLA7 as an anti-biofilm agent in staphylococcal species
url	https://doi.org/10.3389/fmicb.2015.01315 https://doaj.org/article/c2771b23ef0d4252a98801c4cfef9260 http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01315/full https://doaj.org/toc/1664-302X
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up_date	2024-07-04T00:17:24.438Z
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Role of the pre-neck appendage protein (Dpo7) from phage vB_SepiS-phiIPLA7 as an anti-biofilm agent in staphylococcal species

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