DNA sequence variation and determination of the putative PvCSP gene as potential vaccine target for Plasmodium vivax malaria in India

Evolutionary genetic studies in genomes offer excellent opportunities to infer population structure and demographic history of species populations. However, such kinds of studies are very limited in malaria. Malaria is highly endemic in India and both of the causative agents of malaria, Plasmodium vivax and Plasmodium falciparum occur in almost equal proportion. The widespread distribution of P. vivax is attributing to socio-economic loss, and thereby increasing the public health concern. Therefore, it is important to understand the genetic features of P. vivax population in India. Comparative genomics of P. falciparum and P. vivax has revealed several syntenic chromosomal segments. One such 200 kb segment has been utilized to design several small DNA fragments from non-coding regions, and tested for ‘putatively neutral’ marker for inference of population structure and demography of P. vivax. Utilizing 126 P. vivax isolates collected from 10 different widespread geographic locations in India, it was found that two neutral DNA fragments (P10 and P17) showed fairly less nucleotide diversity in all the population samples of P. vivax. A sudden drop in diversity in putatively neutral genetic fragments indicates the role of positive natural selection under the hitchhiking model of molecular evolution. Evolutionary genetic studies in the regions surrounding P10 and P17 with functional validation might provide meaningful insights and help identify targets in P. vivax in India. The study can further extended to P.falciparum as it is syntenic to P.vivax. It was found that the neutral fragment P17 is flanked by putative circumsporozoite protein (PvCSP) gene. Since, CSP is a major surface protein of the infective stage of malaria parasite; it is believed that the PvCSP gene might be under a certain kind of selective pressure. The objective of this study is to obtain new DNA sequence information of the putative PvCSP gene in isolates from India and compare it with the estimated diversity of the non-coding DNA fragments located in-and around this gene for inference of natural selection. Effectiveness of putative PvCSP gene as a suitable vaccine candidate on the basis of genetic diversity in parasite populations can be evaluated. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Manoswini Dash [verfasserIn] Aparup Das [verfasserIn] Abhinav Sinha [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Übergeordnetes Werk:	In: Canadian Journal of Biotechnology - Science Planet Inc., 2017, 1(2017), Special Issue, Seite 88-88
Übergeordnetes Werk:	volume:1 ; year:2017 ; number:Special Issue ; pages:88-88

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.24870/cjb.2017-a75

Katalog-ID:	DOAJ012625892

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allfields	10.24870/cjb.2017-a75 doi (DE-627)DOAJ012625892 (DE-599)DOAJb4ac25bf38bd493d8453c8fe1fac1223 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Manoswini Dash verfasserin aut DNA sequence variation and determination of the putative PvCSP gene as potential vaccine target for Plasmodium vivax malaria in India 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Evolutionary genetic studies in genomes offer excellent opportunities to infer population structure and demographic history of species populations. However, such kinds of studies are very limited in malaria. Malaria is highly endemic in India and both of the causative agents of malaria, Plasmodium vivax and Plasmodium falciparum occur in almost equal proportion. The widespread distribution of P. vivax is attributing to socio-economic loss, and thereby increasing the public health concern. Therefore, it is important to understand the genetic features of P. vivax population in India. Comparative genomics of P. falciparum and P. vivax has revealed several syntenic chromosomal segments. One such 200 kb segment has been utilized to design several small DNA fragments from non-coding regions, and tested for ‘putatively neutral’ marker for inference of population structure and demography of P. vivax. Utilizing 126 P. vivax isolates collected from 10 different widespread geographic locations in India, it was found that two neutral DNA fragments (P10 and P17) showed fairly less nucleotide diversity in all the population samples of P. vivax. A sudden drop in diversity in putatively neutral genetic fragments indicates the role of positive natural selection under the hitchhiking model of molecular evolution. Evolutionary genetic studies in the regions surrounding P10 and P17 with functional validation might provide meaningful insights and help identify targets in P. vivax in India. The study can further extended to P.falciparum as it is syntenic to P.vivax. It was found that the neutral fragment P17 is flanked by putative circumsporozoite protein (PvCSP) gene. Since, CSP is a major surface protein of the infective stage of malaria parasite; it is believed that the PvCSP gene might be under a certain kind of selective pressure. The objective of this study is to obtain new DNA sequence information of the putative PvCSP gene in isolates from India and compare it with the estimated diversity of the non-coding DNA fragments located in-and around this gene for inference of natural selection. Effectiveness of putative PvCSP gene as a suitable vaccine candidate on the basis of genetic diversity in parasite populations can be evaluated. Biotechnology Aparup Das verfasserin aut Abhinav Sinha verfasserin aut In Canadian Journal of Biotechnology Science Planet Inc., 2017 1(2017), Special Issue, Seite 88-88 (DE-627)1023482800 25608304 nnns volume:1 year:2017 number:Special Issue pages:88-88 https://doi.org/10.24870/cjb.2017-a75 kostenfrei https://doaj.org/article/b4ac25bf38bd493d8453c8fe1fac1223 kostenfrei https://www.canadianjbiotech.com/CAN_J_BIOTECH/Archives/v1/Special Issue/cjb.2017-a75.pdf kostenfrei https://doaj.org/toc/2560-8304 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 1 2017 Special Issue 88-88
spelling	10.24870/cjb.2017-a75 doi (DE-627)DOAJ012625892 (DE-599)DOAJb4ac25bf38bd493d8453c8fe1fac1223 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Manoswini Dash verfasserin aut DNA sequence variation and determination of the putative PvCSP gene as potential vaccine target for Plasmodium vivax malaria in India 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Evolutionary genetic studies in genomes offer excellent opportunities to infer population structure and demographic history of species populations. However, such kinds of studies are very limited in malaria. Malaria is highly endemic in India and both of the causative agents of malaria, Plasmodium vivax and Plasmodium falciparum occur in almost equal proportion. The widespread distribution of P. vivax is attributing to socio-economic loss, and thereby increasing the public health concern. Therefore, it is important to understand the genetic features of P. vivax population in India. Comparative genomics of P. falciparum and P. vivax has revealed several syntenic chromosomal segments. One such 200 kb segment has been utilized to design several small DNA fragments from non-coding regions, and tested for ‘putatively neutral’ marker for inference of population structure and demography of P. vivax. Utilizing 126 P. vivax isolates collected from 10 different widespread geographic locations in India, it was found that two neutral DNA fragments (P10 and P17) showed fairly less nucleotide diversity in all the population samples of P. vivax. A sudden drop in diversity in putatively neutral genetic fragments indicates the role of positive natural selection under the hitchhiking model of molecular evolution. Evolutionary genetic studies in the regions surrounding P10 and P17 with functional validation might provide meaningful insights and help identify targets in P. vivax in India. The study can further extended to P.falciparum as it is syntenic to P.vivax. It was found that the neutral fragment P17 is flanked by putative circumsporozoite protein (PvCSP) gene. Since, CSP is a major surface protein of the infective stage of malaria parasite; it is believed that the PvCSP gene might be under a certain kind of selective pressure. The objective of this study is to obtain new DNA sequence information of the putative PvCSP gene in isolates from India and compare it with the estimated diversity of the non-coding DNA fragments located in-and around this gene for inference of natural selection. Effectiveness of putative PvCSP gene as a suitable vaccine candidate on the basis of genetic diversity in parasite populations can be evaluated. Biotechnology Aparup Das verfasserin aut Abhinav Sinha verfasserin aut In Canadian Journal of Biotechnology Science Planet Inc., 2017 1(2017), Special Issue, Seite 88-88 (DE-627)1023482800 25608304 nnns volume:1 year:2017 number:Special Issue pages:88-88 https://doi.org/10.24870/cjb.2017-a75 kostenfrei https://doaj.org/article/b4ac25bf38bd493d8453c8fe1fac1223 kostenfrei https://www.canadianjbiotech.com/CAN_J_BIOTECH/Archives/v1/Special Issue/cjb.2017-a75.pdf kostenfrei https://doaj.org/toc/2560-8304 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 1 2017 Special Issue 88-88
allfields_unstemmed	10.24870/cjb.2017-a75 doi (DE-627)DOAJ012625892 (DE-599)DOAJb4ac25bf38bd493d8453c8fe1fac1223 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Manoswini Dash verfasserin aut DNA sequence variation and determination of the putative PvCSP gene as potential vaccine target for Plasmodium vivax malaria in India 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Evolutionary genetic studies in genomes offer excellent opportunities to infer population structure and demographic history of species populations. However, such kinds of studies are very limited in malaria. Malaria is highly endemic in India and both of the causative agents of malaria, Plasmodium vivax and Plasmodium falciparum occur in almost equal proportion. The widespread distribution of P. vivax is attributing to socio-economic loss, and thereby increasing the public health concern. Therefore, it is important to understand the genetic features of P. vivax population in India. Comparative genomics of P. falciparum and P. vivax has revealed several syntenic chromosomal segments. One such 200 kb segment has been utilized to design several small DNA fragments from non-coding regions, and tested for ‘putatively neutral’ marker for inference of population structure and demography of P. vivax. Utilizing 126 P. vivax isolates collected from 10 different widespread geographic locations in India, it was found that two neutral DNA fragments (P10 and P17) showed fairly less nucleotide diversity in all the population samples of P. vivax. A sudden drop in diversity in putatively neutral genetic fragments indicates the role of positive natural selection under the hitchhiking model of molecular evolution. Evolutionary genetic studies in the regions surrounding P10 and P17 with functional validation might provide meaningful insights and help identify targets in P. vivax in India. The study can further extended to P.falciparum as it is syntenic to P.vivax. It was found that the neutral fragment P17 is flanked by putative circumsporozoite protein (PvCSP) gene. Since, CSP is a major surface protein of the infective stage of malaria parasite; it is believed that the PvCSP gene might be under a certain kind of selective pressure. The objective of this study is to obtain new DNA sequence information of the putative PvCSP gene in isolates from India and compare it with the estimated diversity of the non-coding DNA fragments located in-and around this gene for inference of natural selection. Effectiveness of putative PvCSP gene as a suitable vaccine candidate on the basis of genetic diversity in parasite populations can be evaluated. Biotechnology Aparup Das verfasserin aut Abhinav Sinha verfasserin aut In Canadian Journal of Biotechnology Science Planet Inc., 2017 1(2017), Special Issue, Seite 88-88 (DE-627)1023482800 25608304 nnns volume:1 year:2017 number:Special Issue pages:88-88 https://doi.org/10.24870/cjb.2017-a75 kostenfrei https://doaj.org/article/b4ac25bf38bd493d8453c8fe1fac1223 kostenfrei https://www.canadianjbiotech.com/CAN_J_BIOTECH/Archives/v1/Special Issue/cjb.2017-a75.pdf kostenfrei https://doaj.org/toc/2560-8304 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 1 2017 Special Issue 88-88
allfieldsGer	10.24870/cjb.2017-a75 doi (DE-627)DOAJ012625892 (DE-599)DOAJb4ac25bf38bd493d8453c8fe1fac1223 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Manoswini Dash verfasserin aut DNA sequence variation and determination of the putative PvCSP gene as potential vaccine target for Plasmodium vivax malaria in India 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Evolutionary genetic studies in genomes offer excellent opportunities to infer population structure and demographic history of species populations. However, such kinds of studies are very limited in malaria. Malaria is highly endemic in India and both of the causative agents of malaria, Plasmodium vivax and Plasmodium falciparum occur in almost equal proportion. The widespread distribution of P. vivax is attributing to socio-economic loss, and thereby increasing the public health concern. Therefore, it is important to understand the genetic features of P. vivax population in India. Comparative genomics of P. falciparum and P. vivax has revealed several syntenic chromosomal segments. One such 200 kb segment has been utilized to design several small DNA fragments from non-coding regions, and tested for ‘putatively neutral’ marker for inference of population structure and demography of P. vivax. Utilizing 126 P. vivax isolates collected from 10 different widespread geographic locations in India, it was found that two neutral DNA fragments (P10 and P17) showed fairly less nucleotide diversity in all the population samples of P. vivax. A sudden drop in diversity in putatively neutral genetic fragments indicates the role of positive natural selection under the hitchhiking model of molecular evolution. Evolutionary genetic studies in the regions surrounding P10 and P17 with functional validation might provide meaningful insights and help identify targets in P. vivax in India. The study can further extended to P.falciparum as it is syntenic to P.vivax. It was found that the neutral fragment P17 is flanked by putative circumsporozoite protein (PvCSP) gene. Since, CSP is a major surface protein of the infective stage of malaria parasite; it is believed that the PvCSP gene might be under a certain kind of selective pressure. The objective of this study is to obtain new DNA sequence information of the putative PvCSP gene in isolates from India and compare it with the estimated diversity of the non-coding DNA fragments located in-and around this gene for inference of natural selection. Effectiveness of putative PvCSP gene as a suitable vaccine candidate on the basis of genetic diversity in parasite populations can be evaluated. Biotechnology Aparup Das verfasserin aut Abhinav Sinha verfasserin aut In Canadian Journal of Biotechnology Science Planet Inc., 2017 1(2017), Special Issue, Seite 88-88 (DE-627)1023482800 25608304 nnns volume:1 year:2017 number:Special Issue pages:88-88 https://doi.org/10.24870/cjb.2017-a75 kostenfrei https://doaj.org/article/b4ac25bf38bd493d8453c8fe1fac1223 kostenfrei https://www.canadianjbiotech.com/CAN_J_BIOTECH/Archives/v1/Special Issue/cjb.2017-a75.pdf kostenfrei https://doaj.org/toc/2560-8304 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 1 2017 Special Issue 88-88
allfieldsSound	10.24870/cjb.2017-a75 doi (DE-627)DOAJ012625892 (DE-599)DOAJb4ac25bf38bd493d8453c8fe1fac1223 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Manoswini Dash verfasserin aut DNA sequence variation and determination of the putative PvCSP gene as potential vaccine target for Plasmodium vivax malaria in India 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Evolutionary genetic studies in genomes offer excellent opportunities to infer population structure and demographic history of species populations. However, such kinds of studies are very limited in malaria. Malaria is highly endemic in India and both of the causative agents of malaria, Plasmodium vivax and Plasmodium falciparum occur in almost equal proportion. The widespread distribution of P. vivax is attributing to socio-economic loss, and thereby increasing the public health concern. Therefore, it is important to understand the genetic features of P. vivax population in India. Comparative genomics of P. falciparum and P. vivax has revealed several syntenic chromosomal segments. One such 200 kb segment has been utilized to design several small DNA fragments from non-coding regions, and tested for ‘putatively neutral’ marker for inference of population structure and demography of P. vivax. Utilizing 126 P. vivax isolates collected from 10 different widespread geographic locations in India, it was found that two neutral DNA fragments (P10 and P17) showed fairly less nucleotide diversity in all the population samples of P. vivax. A sudden drop in diversity in putatively neutral genetic fragments indicates the role of positive natural selection under the hitchhiking model of molecular evolution. Evolutionary genetic studies in the regions surrounding P10 and P17 with functional validation might provide meaningful insights and help identify targets in P. vivax in India. The study can further extended to P.falciparum as it is syntenic to P.vivax. It was found that the neutral fragment P17 is flanked by putative circumsporozoite protein (PvCSP) gene. Since, CSP is a major surface protein of the infective stage of malaria parasite; it is believed that the PvCSP gene might be under a certain kind of selective pressure. The objective of this study is to obtain new DNA sequence information of the putative PvCSP gene in isolates from India and compare it with the estimated diversity of the non-coding DNA fragments located in-and around this gene for inference of natural selection. Effectiveness of putative PvCSP gene as a suitable vaccine candidate on the basis of genetic diversity in parasite populations can be evaluated. Biotechnology Aparup Das verfasserin aut Abhinav Sinha verfasserin aut In Canadian Journal of Biotechnology Science Planet Inc., 2017 1(2017), Special Issue, Seite 88-88 (DE-627)1023482800 25608304 nnns volume:1 year:2017 number:Special Issue pages:88-88 https://doi.org/10.24870/cjb.2017-a75 kostenfrei https://doaj.org/article/b4ac25bf38bd493d8453c8fe1fac1223 kostenfrei https://www.canadianjbiotech.com/CAN_J_BIOTECH/Archives/v1/Special Issue/cjb.2017-a75.pdf kostenfrei https://doaj.org/toc/2560-8304 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 1 2017 Special Issue 88-88
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author	Manoswini Dash
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topic_title	TP248.13-248.65 DNA sequence variation and determination of the putative PvCSP gene as potential vaccine target for Plasmodium vivax malaria in India
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title	DNA sequence variation and determination of the putative PvCSP gene as potential vaccine target for Plasmodium vivax malaria in India
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title_full	DNA sequence variation and determination of the putative PvCSP gene as potential vaccine target for Plasmodium vivax malaria in India
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title_sort	dna sequence variation and determination of the putative pvcsp gene as potential vaccine target for plasmodium vivax malaria in india
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title_auth	DNA sequence variation and determination of the putative PvCSP gene as potential vaccine target for Plasmodium vivax malaria in India
abstract	Evolutionary genetic studies in genomes offer excellent opportunities to infer population structure and demographic history of species populations. However, such kinds of studies are very limited in malaria. Malaria is highly endemic in India and both of the causative agents of malaria, Plasmodium vivax and Plasmodium falciparum occur in almost equal proportion. The widespread distribution of P. vivax is attributing to socio-economic loss, and thereby increasing the public health concern. Therefore, it is important to understand the genetic features of P. vivax population in India. Comparative genomics of P. falciparum and P. vivax has revealed several syntenic chromosomal segments. One such 200 kb segment has been utilized to design several small DNA fragments from non-coding regions, and tested for ‘putatively neutral’ marker for inference of population structure and demography of P. vivax. Utilizing 126 P. vivax isolates collected from 10 different widespread geographic locations in India, it was found that two neutral DNA fragments (P10 and P17) showed fairly less nucleotide diversity in all the population samples of P. vivax. A sudden drop in diversity in putatively neutral genetic fragments indicates the role of positive natural selection under the hitchhiking model of molecular evolution. Evolutionary genetic studies in the regions surrounding P10 and P17 with functional validation might provide meaningful insights and help identify targets in P. vivax in India. The study can further extended to P.falciparum as it is syntenic to P.vivax. It was found that the neutral fragment P17 is flanked by putative circumsporozoite protein (PvCSP) gene. Since, CSP is a major surface protein of the infective stage of malaria parasite; it is believed that the PvCSP gene might be under a certain kind of selective pressure. The objective of this study is to obtain new DNA sequence information of the putative PvCSP gene in isolates from India and compare it with the estimated diversity of the non-coding DNA fragments located in-and around this gene for inference of natural selection. Effectiveness of putative PvCSP gene as a suitable vaccine candidate on the basis of genetic diversity in parasite populations can be evaluated.
abstractGer	Evolutionary genetic studies in genomes offer excellent opportunities to infer population structure and demographic history of species populations. However, such kinds of studies are very limited in malaria. Malaria is highly endemic in India and both of the causative agents of malaria, Plasmodium vivax and Plasmodium falciparum occur in almost equal proportion. The widespread distribution of P. vivax is attributing to socio-economic loss, and thereby increasing the public health concern. Therefore, it is important to understand the genetic features of P. vivax population in India. Comparative genomics of P. falciparum and P. vivax has revealed several syntenic chromosomal segments. One such 200 kb segment has been utilized to design several small DNA fragments from non-coding regions, and tested for ‘putatively neutral’ marker for inference of population structure and demography of P. vivax. Utilizing 126 P. vivax isolates collected from 10 different widespread geographic locations in India, it was found that two neutral DNA fragments (P10 and P17) showed fairly less nucleotide diversity in all the population samples of P. vivax. A sudden drop in diversity in putatively neutral genetic fragments indicates the role of positive natural selection under the hitchhiking model of molecular evolution. Evolutionary genetic studies in the regions surrounding P10 and P17 with functional validation might provide meaningful insights and help identify targets in P. vivax in India. The study can further extended to P.falciparum as it is syntenic to P.vivax. It was found that the neutral fragment P17 is flanked by putative circumsporozoite protein (PvCSP) gene. Since, CSP is a major surface protein of the infective stage of malaria parasite; it is believed that the PvCSP gene might be under a certain kind of selective pressure. The objective of this study is to obtain new DNA sequence information of the putative PvCSP gene in isolates from India and compare it with the estimated diversity of the non-coding DNA fragments located in-and around this gene for inference of natural selection. Effectiveness of putative PvCSP gene as a suitable vaccine candidate on the basis of genetic diversity in parasite populations can be evaluated.
abstract_unstemmed	Evolutionary genetic studies in genomes offer excellent opportunities to infer population structure and demographic history of species populations. However, such kinds of studies are very limited in malaria. Malaria is highly endemic in India and both of the causative agents of malaria, Plasmodium vivax and Plasmodium falciparum occur in almost equal proportion. The widespread distribution of P. vivax is attributing to socio-economic loss, and thereby increasing the public health concern. Therefore, it is important to understand the genetic features of P. vivax population in India. Comparative genomics of P. falciparum and P. vivax has revealed several syntenic chromosomal segments. One such 200 kb segment has been utilized to design several small DNA fragments from non-coding regions, and tested for ‘putatively neutral’ marker for inference of population structure and demography of P. vivax. Utilizing 126 P. vivax isolates collected from 10 different widespread geographic locations in India, it was found that two neutral DNA fragments (P10 and P17) showed fairly less nucleotide diversity in all the population samples of P. vivax. A sudden drop in diversity in putatively neutral genetic fragments indicates the role of positive natural selection under the hitchhiking model of molecular evolution. Evolutionary genetic studies in the regions surrounding P10 and P17 with functional validation might provide meaningful insights and help identify targets in P. vivax in India. The study can further extended to P.falciparum as it is syntenic to P.vivax. It was found that the neutral fragment P17 is flanked by putative circumsporozoite protein (PvCSP) gene. Since, CSP is a major surface protein of the infective stage of malaria parasite; it is believed that the PvCSP gene might be under a certain kind of selective pressure. The objective of this study is to obtain new DNA sequence information of the putative PvCSP gene in isolates from India and compare it with the estimated diversity of the non-coding DNA fragments located in-and around this gene for inference of natural selection. Effectiveness of putative PvCSP gene as a suitable vaccine candidate on the basis of genetic diversity in parasite populations can be evaluated.
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title_short	DNA sequence variation and determination of the putative PvCSP gene as potential vaccine target for Plasmodium vivax malaria in India
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The objective of this study is to obtain new DNA sequence information of the putative PvCSP gene in isolates from India and compare it with the estimated diversity of the non-coding DNA fragments located in-and around this gene for inference of natural selection. 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DNA sequence variation and determination of the putative PvCSP gene as potential vaccine target for Plasmodium vivax malaria in India

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