Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) <br< Avaliação da fitotoxicidade de fungicidas e germicida na propagação in vitro de Oncidium varicosum Lindl. (Orchidaceae) para o controle de microorganismos

One of the greatest problem of in vitro production of seedling is the contamination of the culture medium during the micropropagation stages. This study was carried out to investigate the effect of fungicides and germicide on the plant growth and rooting, degree of phytotoxicity and control of contamination during in vitro cultivation of O. varicosum orchid. The flowers were pollinated artificially and the capsules seeds were sowed in MS culture medium modified with half of the macronutrients. The seedlings were transferred to the same culture medium uded for germination, added with the fungicides azoxystrobin, benomyl, chlorothalonil ( 0 g.L-1 , 0.1 g.L-1 ; 0.2 g.L-1 ; 0.4 g.L-1 ; 0.6 g.L-1 and 0.8 g.L-1) or sodium hypochloride with 10% active chloride ( 0 mL. L-1 , 0,5 mL.L-1 ; 0,75 mL. L-1 ; 1,00 mL. L-1 ; 1,25 mL. L-1 ; 1,50 mL. L-1). A complete randomized block design was used with ten replications per treatment and five plantlets per flask. The treatment containing 1.5 mL L-1 sodium hypochloride was the best in preventing contamination by microorganisms and did not cause any apparent harm development and in vitro rooting of Oncidium varicosum plantlets.<p<<p<Um dos maiores problemas na produção de mudas in vitro, é a contaminação por fungos e bactérias do meio de cultura durante as etapas de micropropagação, mesmo havendo todos os cuidados de assepsia. O presente trabalho teve por objetivo avaliar o efeito de fungicidas e germicida no crescimento vegetativo e enraizamento in vitro da orquídea Oncidium varicosum Lindl. As flores foram polinizadas artificialmente e as sementes foram germinadas no meio MS modificado com a metade da concentração dos macronutrientes. As plântulas obtidas foram subcultivadas no mesmo meio de germinação, adicionando-se os fungicidas azoxystrobin, benomyl, chlorothalonil nas concentrações: 0,1 g.L-1; 0,2 g.L-1 ; 0,4 g.L-1; 0,6 g .L-1 e 0,8 g.L-1 O germicida hipoclorito de sódio com 10% de cloro ativo foi adicionado ao meio nutritivo nas concentrações: 0,5 mL. L-1; 0,75 mL .L-1 ; 1,00 mL .L-1 ; 1,25 mL. L-1 e 1,50 mL. L-1 . O delineamento experimental foi o inteiramente casualizado, com dez repetições por tratamento e cinco plântulas por frasco. O tratamento contendo hipoclorito de sódio 1,5 mL . L-1 mostrou-se o menos tóxico, pois não afetou o desenvolvimento vegetativo e o enraizamento in vitro de plântulas de Oncidium varicosum. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Geraldo Lopes Silva [verfasserIn] Inês Cristina Batista Fonseca [verfasserIn] Ricardo Tadeu de Faria [verfasserIn] Melissa Lombardi Oda [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; Portugiesisch

Erschienen:	2003

Schlagwörter:	Cultura de tecidos Meio de cultura Orquídea Fungos Bactérias.

Übergeordnetes Werk:	In: Semina: Ciências Agrárias - Universidade Estadual de Londrina, 2010, 24(2003), 2, Seite 273-276
Übergeordnetes Werk:	volume:24 ; year:2003 ; number:2 ; pages:273-276

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.5433/1679-0359.2003v24n2p273

Katalog-ID:	DOAJ012905739

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allfields	10.5433/1679-0359.2003v24n2p273 doi (DE-627)DOAJ012905739 (DE-599)DOAJ38ea77c1aee9472bab74162fdd61eb91 DE-627 ger DE-627 rakwb eng por S1-972 Geraldo Lopes Silva verfasserin aut Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) <br< Avaliação da fitotoxicidade de fungicidas e germicida na propagação in vitro de Oncidium varicosum Lindl. (Orchidaceae) para o controle de microorganismos 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier One of the greatest problem of in vitro production of seedling is the contamination of the culture medium during the micropropagation stages. This study was carried out to investigate the effect of fungicides and germicide on the plant growth and rooting, degree of phytotoxicity and control of contamination during in vitro cultivation of O. varicosum orchid. The flowers were pollinated artificially and the capsules seeds were sowed in MS culture medium modified with half of the macronutrients. The seedlings were transferred to the same culture medium uded for germination, added with the fungicides azoxystrobin, benomyl, chlorothalonil ( 0 g.L-1 , 0.1 g.L-1 ; 0.2 g.L-1 ; 0.4 g.L-1 ; 0.6 g.L-1 and 0.8 g.L-1) or sodium hypochloride with 10% active chloride ( 0 mL. L-1 , 0,5 mL.L-1 ; 0,75 mL. L-1 ; 1,00 mL. L-1 ; 1,25 mL. L-1 ; 1,50 mL. L-1). A complete randomized block design was used with ten replications per treatment and five plantlets per flask. The treatment containing 1.5 mL L-1 sodium hypochloride was the best in preventing contamination by microorganisms and did not cause any apparent harm development and in vitro rooting of Oncidium varicosum plantlets.<p<<p<Um dos maiores problemas na produção de mudas in vitro, é a contaminação por fungos e bactérias do meio de cultura durante as etapas de micropropagação, mesmo havendo todos os cuidados de assepsia. O presente trabalho teve por objetivo avaliar o efeito de fungicidas e germicida no crescimento vegetativo e enraizamento in vitro da orquídea Oncidium varicosum Lindl. As flores foram polinizadas artificialmente e as sementes foram germinadas no meio MS modificado com a metade da concentração dos macronutrientes. As plântulas obtidas foram subcultivadas no mesmo meio de germinação, adicionando-se os fungicidas azoxystrobin, benomyl, chlorothalonil nas concentrações: 0,1 g.L-1; 0,2 g.L-1 ; 0,4 g.L-1; 0,6 g .L-1 e 0,8 g.L-1 O germicida hipoclorito de sódio com 10% de cloro ativo foi adicionado ao meio nutritivo nas concentrações: 0,5 mL. L-1; 0,75 mL .L-1 ; 1,00 mL .L-1 ; 1,25 mL. L-1 e 1,50 mL. L-1 . O delineamento experimental foi o inteiramente casualizado, com dez repetições por tratamento e cinco plântulas por frasco. O tratamento contendo hipoclorito de sódio 1,5 mL . L-1 mostrou-se o menos tóxico, pois não afetou o desenvolvimento vegetativo e o enraizamento in vitro de plântulas de Oncidium varicosum. Cultura de tecidos Meio de cultura Orquídea Fungos Bactérias. Agriculture (General) Inês Cristina Batista Fonseca verfasserin aut Ricardo Tadeu de Faria verfasserin aut Melissa Lombardi Oda verfasserin aut In Semina: Ciências Agrárias Universidade Estadual de Londrina, 2010 24(2003), 2, Seite 273-276 (DE-627)625424700 (DE-600)2551446-5 16790359 nnns volume:24 year:2003 number:2 pages:273-276 https://doi.org/10.5433/1679-0359.2003v24n2p273 kostenfrei https://doaj.org/article/38ea77c1aee9472bab74162fdd61eb91 kostenfrei http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/2187 kostenfrei https://doaj.org/toc/1676-546X Journal toc kostenfrei https://doaj.org/toc/1679-0359 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 24 2003 2 273-276
spelling	10.5433/1679-0359.2003v24n2p273 doi (DE-627)DOAJ012905739 (DE-599)DOAJ38ea77c1aee9472bab74162fdd61eb91 DE-627 ger DE-627 rakwb eng por S1-972 Geraldo Lopes Silva verfasserin aut Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) <br< Avaliação da fitotoxicidade de fungicidas e germicida na propagação in vitro de Oncidium varicosum Lindl. (Orchidaceae) para o controle de microorganismos 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier One of the greatest problem of in vitro production of seedling is the contamination of the culture medium during the micropropagation stages. This study was carried out to investigate the effect of fungicides and germicide on the plant growth and rooting, degree of phytotoxicity and control of contamination during in vitro cultivation of O. varicosum orchid. The flowers were pollinated artificially and the capsules seeds were sowed in MS culture medium modified with half of the macronutrients. The seedlings were transferred to the same culture medium uded for germination, added with the fungicides azoxystrobin, benomyl, chlorothalonil ( 0 g.L-1 , 0.1 g.L-1 ; 0.2 g.L-1 ; 0.4 g.L-1 ; 0.6 g.L-1 and 0.8 g.L-1) or sodium hypochloride with 10% active chloride ( 0 mL. L-1 , 0,5 mL.L-1 ; 0,75 mL. L-1 ; 1,00 mL. L-1 ; 1,25 mL. L-1 ; 1,50 mL. L-1). A complete randomized block design was used with ten replications per treatment and five plantlets per flask. The treatment containing 1.5 mL L-1 sodium hypochloride was the best in preventing contamination by microorganisms and did not cause any apparent harm development and in vitro rooting of Oncidium varicosum plantlets.<p<<p<Um dos maiores problemas na produção de mudas in vitro, é a contaminação por fungos e bactérias do meio de cultura durante as etapas de micropropagação, mesmo havendo todos os cuidados de assepsia. O presente trabalho teve por objetivo avaliar o efeito de fungicidas e germicida no crescimento vegetativo e enraizamento in vitro da orquídea Oncidium varicosum Lindl. As flores foram polinizadas artificialmente e as sementes foram germinadas no meio MS modificado com a metade da concentração dos macronutrientes. As plântulas obtidas foram subcultivadas no mesmo meio de germinação, adicionando-se os fungicidas azoxystrobin, benomyl, chlorothalonil nas concentrações: 0,1 g.L-1; 0,2 g.L-1 ; 0,4 g.L-1; 0,6 g .L-1 e 0,8 g.L-1 O germicida hipoclorito de sódio com 10% de cloro ativo foi adicionado ao meio nutritivo nas concentrações: 0,5 mL. L-1; 0,75 mL .L-1 ; 1,00 mL .L-1 ; 1,25 mL. L-1 e 1,50 mL. L-1 . O delineamento experimental foi o inteiramente casualizado, com dez repetições por tratamento e cinco plântulas por frasco. O tratamento contendo hipoclorito de sódio 1,5 mL . L-1 mostrou-se o menos tóxico, pois não afetou o desenvolvimento vegetativo e o enraizamento in vitro de plântulas de Oncidium varicosum. Cultura de tecidos Meio de cultura Orquídea Fungos Bactérias. Agriculture (General) Inês Cristina Batista Fonseca verfasserin aut Ricardo Tadeu de Faria verfasserin aut Melissa Lombardi Oda verfasserin aut In Semina: Ciências Agrárias Universidade Estadual de Londrina, 2010 24(2003), 2, Seite 273-276 (DE-627)625424700 (DE-600)2551446-5 16790359 nnns volume:24 year:2003 number:2 pages:273-276 https://doi.org/10.5433/1679-0359.2003v24n2p273 kostenfrei https://doaj.org/article/38ea77c1aee9472bab74162fdd61eb91 kostenfrei http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/2187 kostenfrei https://doaj.org/toc/1676-546X Journal toc kostenfrei https://doaj.org/toc/1679-0359 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 24 2003 2 273-276
allfields_unstemmed	10.5433/1679-0359.2003v24n2p273 doi (DE-627)DOAJ012905739 (DE-599)DOAJ38ea77c1aee9472bab74162fdd61eb91 DE-627 ger DE-627 rakwb eng por S1-972 Geraldo Lopes Silva verfasserin aut Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) <br< Avaliação da fitotoxicidade de fungicidas e germicida na propagação in vitro de Oncidium varicosum Lindl. (Orchidaceae) para o controle de microorganismos 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier One of the greatest problem of in vitro production of seedling is the contamination of the culture medium during the micropropagation stages. This study was carried out to investigate the effect of fungicides and germicide on the plant growth and rooting, degree of phytotoxicity and control of contamination during in vitro cultivation of O. varicosum orchid. The flowers were pollinated artificially and the capsules seeds were sowed in MS culture medium modified with half of the macronutrients. The seedlings were transferred to the same culture medium uded for germination, added with the fungicides azoxystrobin, benomyl, chlorothalonil ( 0 g.L-1 , 0.1 g.L-1 ; 0.2 g.L-1 ; 0.4 g.L-1 ; 0.6 g.L-1 and 0.8 g.L-1) or sodium hypochloride with 10% active chloride ( 0 mL. L-1 , 0,5 mL.L-1 ; 0,75 mL. L-1 ; 1,00 mL. L-1 ; 1,25 mL. L-1 ; 1,50 mL. L-1). A complete randomized block design was used with ten replications per treatment and five plantlets per flask. The treatment containing 1.5 mL L-1 sodium hypochloride was the best in preventing contamination by microorganisms and did not cause any apparent harm development and in vitro rooting of Oncidium varicosum plantlets.<p<<p<Um dos maiores problemas na produção de mudas in vitro, é a contaminação por fungos e bactérias do meio de cultura durante as etapas de micropropagação, mesmo havendo todos os cuidados de assepsia. O presente trabalho teve por objetivo avaliar o efeito de fungicidas e germicida no crescimento vegetativo e enraizamento in vitro da orquídea Oncidium varicosum Lindl. As flores foram polinizadas artificialmente e as sementes foram germinadas no meio MS modificado com a metade da concentração dos macronutrientes. As plântulas obtidas foram subcultivadas no mesmo meio de germinação, adicionando-se os fungicidas azoxystrobin, benomyl, chlorothalonil nas concentrações: 0,1 g.L-1; 0,2 g.L-1 ; 0,4 g.L-1; 0,6 g .L-1 e 0,8 g.L-1 O germicida hipoclorito de sódio com 10% de cloro ativo foi adicionado ao meio nutritivo nas concentrações: 0,5 mL. L-1; 0,75 mL .L-1 ; 1,00 mL .L-1 ; 1,25 mL. L-1 e 1,50 mL. L-1 . O delineamento experimental foi o inteiramente casualizado, com dez repetições por tratamento e cinco plântulas por frasco. O tratamento contendo hipoclorito de sódio 1,5 mL . L-1 mostrou-se o menos tóxico, pois não afetou o desenvolvimento vegetativo e o enraizamento in vitro de plântulas de Oncidium varicosum. Cultura de tecidos Meio de cultura Orquídea Fungos Bactérias. Agriculture (General) Inês Cristina Batista Fonseca verfasserin aut Ricardo Tadeu de Faria verfasserin aut Melissa Lombardi Oda verfasserin aut In Semina: Ciências Agrárias Universidade Estadual de Londrina, 2010 24(2003), 2, Seite 273-276 (DE-627)625424700 (DE-600)2551446-5 16790359 nnns volume:24 year:2003 number:2 pages:273-276 https://doi.org/10.5433/1679-0359.2003v24n2p273 kostenfrei https://doaj.org/article/38ea77c1aee9472bab74162fdd61eb91 kostenfrei http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/2187 kostenfrei https://doaj.org/toc/1676-546X Journal toc kostenfrei https://doaj.org/toc/1679-0359 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 24 2003 2 273-276
allfieldsGer	10.5433/1679-0359.2003v24n2p273 doi (DE-627)DOAJ012905739 (DE-599)DOAJ38ea77c1aee9472bab74162fdd61eb91 DE-627 ger DE-627 rakwb eng por S1-972 Geraldo Lopes Silva verfasserin aut Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) <br< Avaliação da fitotoxicidade de fungicidas e germicida na propagação in vitro de Oncidium varicosum Lindl. (Orchidaceae) para o controle de microorganismos 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier One of the greatest problem of in vitro production of seedling is the contamination of the culture medium during the micropropagation stages. This study was carried out to investigate the effect of fungicides and germicide on the plant growth and rooting, degree of phytotoxicity and control of contamination during in vitro cultivation of O. varicosum orchid. The flowers were pollinated artificially and the capsules seeds were sowed in MS culture medium modified with half of the macronutrients. The seedlings were transferred to the same culture medium uded for germination, added with the fungicides azoxystrobin, benomyl, chlorothalonil ( 0 g.L-1 , 0.1 g.L-1 ; 0.2 g.L-1 ; 0.4 g.L-1 ; 0.6 g.L-1 and 0.8 g.L-1) or sodium hypochloride with 10% active chloride ( 0 mL. L-1 , 0,5 mL.L-1 ; 0,75 mL. L-1 ; 1,00 mL. L-1 ; 1,25 mL. L-1 ; 1,50 mL. L-1). A complete randomized block design was used with ten replications per treatment and five plantlets per flask. The treatment containing 1.5 mL L-1 sodium hypochloride was the best in preventing contamination by microorganisms and did not cause any apparent harm development and in vitro rooting of Oncidium varicosum plantlets.<p<<p<Um dos maiores problemas na produção de mudas in vitro, é a contaminação por fungos e bactérias do meio de cultura durante as etapas de micropropagação, mesmo havendo todos os cuidados de assepsia. O presente trabalho teve por objetivo avaliar o efeito de fungicidas e germicida no crescimento vegetativo e enraizamento in vitro da orquídea Oncidium varicosum Lindl. As flores foram polinizadas artificialmente e as sementes foram germinadas no meio MS modificado com a metade da concentração dos macronutrientes. As plântulas obtidas foram subcultivadas no mesmo meio de germinação, adicionando-se os fungicidas azoxystrobin, benomyl, chlorothalonil nas concentrações: 0,1 g.L-1; 0,2 g.L-1 ; 0,4 g.L-1; 0,6 g .L-1 e 0,8 g.L-1 O germicida hipoclorito de sódio com 10% de cloro ativo foi adicionado ao meio nutritivo nas concentrações: 0,5 mL. L-1; 0,75 mL .L-1 ; 1,00 mL .L-1 ; 1,25 mL. L-1 e 1,50 mL. L-1 . O delineamento experimental foi o inteiramente casualizado, com dez repetições por tratamento e cinco plântulas por frasco. O tratamento contendo hipoclorito de sódio 1,5 mL . L-1 mostrou-se o menos tóxico, pois não afetou o desenvolvimento vegetativo e o enraizamento in vitro de plântulas de Oncidium varicosum. Cultura de tecidos Meio de cultura Orquídea Fungos Bactérias. Agriculture (General) Inês Cristina Batista Fonseca verfasserin aut Ricardo Tadeu de Faria verfasserin aut Melissa Lombardi Oda verfasserin aut In Semina: Ciências Agrárias Universidade Estadual de Londrina, 2010 24(2003), 2, Seite 273-276 (DE-627)625424700 (DE-600)2551446-5 16790359 nnns volume:24 year:2003 number:2 pages:273-276 https://doi.org/10.5433/1679-0359.2003v24n2p273 kostenfrei https://doaj.org/article/38ea77c1aee9472bab74162fdd61eb91 kostenfrei http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/2187 kostenfrei https://doaj.org/toc/1676-546X Journal toc kostenfrei https://doaj.org/toc/1679-0359 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 24 2003 2 273-276
allfieldsSound	10.5433/1679-0359.2003v24n2p273 doi (DE-627)DOAJ012905739 (DE-599)DOAJ38ea77c1aee9472bab74162fdd61eb91 DE-627 ger DE-627 rakwb eng por S1-972 Geraldo Lopes Silva verfasserin aut Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) <br< Avaliação da fitotoxicidade de fungicidas e germicida na propagação in vitro de Oncidium varicosum Lindl. (Orchidaceae) para o controle de microorganismos 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier One of the greatest problem of in vitro production of seedling is the contamination of the culture medium during the micropropagation stages. This study was carried out to investigate the effect of fungicides and germicide on the plant growth and rooting, degree of phytotoxicity and control of contamination during in vitro cultivation of O. varicosum orchid. The flowers were pollinated artificially and the capsules seeds were sowed in MS culture medium modified with half of the macronutrients. The seedlings were transferred to the same culture medium uded for germination, added with the fungicides azoxystrobin, benomyl, chlorothalonil ( 0 g.L-1 , 0.1 g.L-1 ; 0.2 g.L-1 ; 0.4 g.L-1 ; 0.6 g.L-1 and 0.8 g.L-1) or sodium hypochloride with 10% active chloride ( 0 mL. L-1 , 0,5 mL.L-1 ; 0,75 mL. L-1 ; 1,00 mL. L-1 ; 1,25 mL. L-1 ; 1,50 mL. L-1). A complete randomized block design was used with ten replications per treatment and five plantlets per flask. The treatment containing 1.5 mL L-1 sodium hypochloride was the best in preventing contamination by microorganisms and did not cause any apparent harm development and in vitro rooting of Oncidium varicosum plantlets.<p<<p<Um dos maiores problemas na produção de mudas in vitro, é a contaminação por fungos e bactérias do meio de cultura durante as etapas de micropropagação, mesmo havendo todos os cuidados de assepsia. O presente trabalho teve por objetivo avaliar o efeito de fungicidas e germicida no crescimento vegetativo e enraizamento in vitro da orquídea Oncidium varicosum Lindl. As flores foram polinizadas artificialmente e as sementes foram germinadas no meio MS modificado com a metade da concentração dos macronutrientes. As plântulas obtidas foram subcultivadas no mesmo meio de germinação, adicionando-se os fungicidas azoxystrobin, benomyl, chlorothalonil nas concentrações: 0,1 g.L-1; 0,2 g.L-1 ; 0,4 g.L-1; 0,6 g .L-1 e 0,8 g.L-1 O germicida hipoclorito de sódio com 10% de cloro ativo foi adicionado ao meio nutritivo nas concentrações: 0,5 mL. L-1; 0,75 mL .L-1 ; 1,00 mL .L-1 ; 1,25 mL. L-1 e 1,50 mL. L-1 . O delineamento experimental foi o inteiramente casualizado, com dez repetições por tratamento e cinco plântulas por frasco. O tratamento contendo hipoclorito de sódio 1,5 mL . L-1 mostrou-se o menos tóxico, pois não afetou o desenvolvimento vegetativo e o enraizamento in vitro de plântulas de Oncidium varicosum. Cultura de tecidos Meio de cultura Orquídea Fungos Bactérias. Agriculture (General) Inês Cristina Batista Fonseca verfasserin aut Ricardo Tadeu de Faria verfasserin aut Melissa Lombardi Oda verfasserin aut In Semina: Ciências Agrárias Universidade Estadual de Londrina, 2010 24(2003), 2, Seite 273-276 (DE-627)625424700 (DE-600)2551446-5 16790359 nnns volume:24 year:2003 number:2 pages:273-276 https://doi.org/10.5433/1679-0359.2003v24n2p273 kostenfrei https://doaj.org/article/38ea77c1aee9472bab74162fdd61eb91 kostenfrei http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/2187 kostenfrei https://doaj.org/toc/1676-546X Journal toc kostenfrei https://doaj.org/toc/1679-0359 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 24 2003 2 273-276
language	English Portuguese
source	In Semina: Ciências Agrárias 24(2003), 2, Seite 273-276 volume:24 year:2003 number:2 pages:273-276
sourceStr	In Semina: Ciências Agrárias 24(2003), 2, Seite 273-276 volume:24 year:2003 number:2 pages:273-276
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topic_facet	Cultura de tecidos Meio de cultura Orquídea Fungos Bactérias. Agriculture (General)
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container_title	Semina: Ciências Agrárias
authorswithroles_txt_mv	Geraldo Lopes Silva @@aut@@ Inês Cristina Batista Fonseca @@aut@@ Ricardo Tadeu de Faria @@aut@@ Melissa Lombardi Oda @@aut@@
publishDateDaySort_date	2003-01-01T00:00:00Z
hierarchy_top_id	625424700
id	DOAJ012905739
language_de	englisch portugiesisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ012905739</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230501182228.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230225s2003 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.5433/1679-0359.2003v24n2p273</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ012905739</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ38ea77c1aee9472bab74162fdd61eb91</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield><subfield code="a">por</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">S1-972</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Geraldo Lopes Silva</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) <br< Avaliação da fitotoxicidade de fungicidas e germicida na propagação in vitro de Oncidium varicosum Lindl. (Orchidaceae) para o controle de microorganismos</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2003</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">One of the greatest problem of in vitro production of seedling is the contamination of the culture medium during the micropropagation stages. This study was carried out to investigate the effect of fungicides and germicide on the plant growth and rooting, degree of phytotoxicity and control of contamination during in vitro cultivation of O. varicosum orchid. The flowers were pollinated artificially and the capsules seeds were sowed in MS culture medium modified with half of the macronutrients. The seedlings were transferred to the same culture medium uded for germination, added with the fungicides azoxystrobin, benomyl, chlorothalonil ( 0 g.L-1 , 0.1 g.L-1 ; 0.2 g.L-1 ; 0.4 g.L-1 ; 0.6 g.L-1 and 0.8 g.L-1) or sodium hypochloride with 10% active chloride ( 0 mL. L-1 , 0,5 mL.L-1 ; 0,75 mL. L-1 ; 1,00 mL. L-1 ; 1,25 mL. L-1 ; 1,50 mL. L-1). A complete randomized block design was used with ten replications per treatment and five plantlets per flask. The treatment containing 1.5 mL L-1 sodium hypochloride was the best in preventing contamination by microorganisms and did not cause any apparent harm development and in vitro rooting of Oncidium varicosum plantlets.<p<<p<Um dos maiores problemas na produção de mudas in vitro, é a contaminação por fungos e bactérias do meio de cultura durante as etapas de micropropagação, mesmo havendo todos os cuidados de assepsia. O presente trabalho teve por objetivo avaliar o efeito de fungicidas e germicida no crescimento vegetativo e enraizamento in vitro da orquídea Oncidium varicosum Lindl. As flores foram polinizadas artificialmente e as sementes foram germinadas no meio MS modificado com a metade da concentração dos macronutrientes. As plântulas obtidas foram subcultivadas no mesmo meio de germinação, adicionando-se os fungicidas azoxystrobin, benomyl, chlorothalonil nas concentrações: 0,1 g.L-1; 0,2 g.L-1 ; 0,4 g.L-1; 0,6 g .L-1 e 0,8 g.L-1 O germicida hipoclorito de sódio com 10% de cloro ativo foi adicionado ao meio nutritivo nas concentrações: 0,5 mL. L-1; 0,75 mL .L-1 ; 1,00 mL .L-1 ; 1,25 mL. L-1 e 1,50 mL. L-1 . O delineamento experimental foi o inteiramente casualizado, com dez repetições por tratamento e cinco plântulas por frasco. O tratamento contendo hipoclorito de sódio 1,5 mL . 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author	Geraldo Lopes Silva
spellingShingle	Geraldo Lopes Silva misc S1-972 misc Cultura de tecidos misc Meio de cultura misc Orquídea misc Fungos misc Bactérias. misc Agriculture (General) Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) <br< Avaliação da fitotoxicidade de fungicidas e germicida na propagação in vitro de Oncidium varicosum Lindl. (Orchidaceae) para o controle de microorganismos
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topic_title	S1-972 Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) <br< Avaliação da fitotoxicidade de fungicidas e germicida na propagação in vitro de Oncidium varicosum Lindl. (Orchidaceae) para o controle de microorganismos Cultura de tecidos Meio de cultura Orquídea Fungos Bactérias
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title	Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) <br< Avaliação da fitotoxicidade de fungicidas e germicida na propagação in vitro de Oncidium varicosum Lindl. (Orchidaceae) para o controle de microorganismos
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title_full	Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) <br< Avaliação da fitotoxicidade de fungicidas e germicida na propagação in vitro de Oncidium varicosum Lindl. (Orchidaceae) para o controle de microorganismos
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title_sort	fungicide and germicide on contamination escaping in the in vitro propagation of oncidium varicosum lindl. (orchidaceae) <br< avaliação da fitotoxicidade de fungicidas e germicida na propagação in vitro de oncidium varicosum lindl. (orchidaceae) para o controle de microorganismos
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title_auth	Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) <br< Avaliação da fitotoxicidade de fungicidas e germicida na propagação in vitro de Oncidium varicosum Lindl. (Orchidaceae) para o controle de microorganismos
abstract	One of the greatest problem of in vitro production of seedling is the contamination of the culture medium during the micropropagation stages. This study was carried out to investigate the effect of fungicides and germicide on the plant growth and rooting, degree of phytotoxicity and control of contamination during in vitro cultivation of O. varicosum orchid. The flowers were pollinated artificially and the capsules seeds were sowed in MS culture medium modified with half of the macronutrients. The seedlings were transferred to the same culture medium uded for germination, added with the fungicides azoxystrobin, benomyl, chlorothalonil ( 0 g.L-1 , 0.1 g.L-1 ; 0.2 g.L-1 ; 0.4 g.L-1 ; 0.6 g.L-1 and 0.8 g.L-1) or sodium hypochloride with 10% active chloride ( 0 mL. L-1 , 0,5 mL.L-1 ; 0,75 mL. L-1 ; 1,00 mL. L-1 ; 1,25 mL. L-1 ; 1,50 mL. L-1). A complete randomized block design was used with ten replications per treatment and five plantlets per flask. The treatment containing 1.5 mL L-1 sodium hypochloride was the best in preventing contamination by microorganisms and did not cause any apparent harm development and in vitro rooting of Oncidium varicosum plantlets.<p<<p<Um dos maiores problemas na produção de mudas in vitro, é a contaminação por fungos e bactérias do meio de cultura durante as etapas de micropropagação, mesmo havendo todos os cuidados de assepsia. O presente trabalho teve por objetivo avaliar o efeito de fungicidas e germicida no crescimento vegetativo e enraizamento in vitro da orquídea Oncidium varicosum Lindl. As flores foram polinizadas artificialmente e as sementes foram germinadas no meio MS modificado com a metade da concentração dos macronutrientes. As plântulas obtidas foram subcultivadas no mesmo meio de germinação, adicionando-se os fungicidas azoxystrobin, benomyl, chlorothalonil nas concentrações: 0,1 g.L-1; 0,2 g.L-1 ; 0,4 g.L-1; 0,6 g .L-1 e 0,8 g.L-1 O germicida hipoclorito de sódio com 10% de cloro ativo foi adicionado ao meio nutritivo nas concentrações: 0,5 mL. L-1; 0,75 mL .L-1 ; 1,00 mL .L-1 ; 1,25 mL. L-1 e 1,50 mL. L-1 . O delineamento experimental foi o inteiramente casualizado, com dez repetições por tratamento e cinco plântulas por frasco. O tratamento contendo hipoclorito de sódio 1,5 mL . L-1 mostrou-se o menos tóxico, pois não afetou o desenvolvimento vegetativo e o enraizamento in vitro de plântulas de Oncidium varicosum.
abstractGer	One of the greatest problem of in vitro production of seedling is the contamination of the culture medium during the micropropagation stages. This study was carried out to investigate the effect of fungicides and germicide on the plant growth and rooting, degree of phytotoxicity and control of contamination during in vitro cultivation of O. varicosum orchid. The flowers were pollinated artificially and the capsules seeds were sowed in MS culture medium modified with half of the macronutrients. The seedlings were transferred to the same culture medium uded for germination, added with the fungicides azoxystrobin, benomyl, chlorothalonil ( 0 g.L-1 , 0.1 g.L-1 ; 0.2 g.L-1 ; 0.4 g.L-1 ; 0.6 g.L-1 and 0.8 g.L-1) or sodium hypochloride with 10% active chloride ( 0 mL. L-1 , 0,5 mL.L-1 ; 0,75 mL. L-1 ; 1,00 mL. L-1 ; 1,25 mL. L-1 ; 1,50 mL. L-1). A complete randomized block design was used with ten replications per treatment and five plantlets per flask. The treatment containing 1.5 mL L-1 sodium hypochloride was the best in preventing contamination by microorganisms and did not cause any apparent harm development and in vitro rooting of Oncidium varicosum plantlets.<p<<p<Um dos maiores problemas na produção de mudas in vitro, é a contaminação por fungos e bactérias do meio de cultura durante as etapas de micropropagação, mesmo havendo todos os cuidados de assepsia. O presente trabalho teve por objetivo avaliar o efeito de fungicidas e germicida no crescimento vegetativo e enraizamento in vitro da orquídea Oncidium varicosum Lindl. As flores foram polinizadas artificialmente e as sementes foram germinadas no meio MS modificado com a metade da concentração dos macronutrientes. As plântulas obtidas foram subcultivadas no mesmo meio de germinação, adicionando-se os fungicidas azoxystrobin, benomyl, chlorothalonil nas concentrações: 0,1 g.L-1; 0,2 g.L-1 ; 0,4 g.L-1; 0,6 g .L-1 e 0,8 g.L-1 O germicida hipoclorito de sódio com 10% de cloro ativo foi adicionado ao meio nutritivo nas concentrações: 0,5 mL. L-1; 0,75 mL .L-1 ; 1,00 mL .L-1 ; 1,25 mL. L-1 e 1,50 mL. L-1 . O delineamento experimental foi o inteiramente casualizado, com dez repetições por tratamento e cinco plântulas por frasco. O tratamento contendo hipoclorito de sódio 1,5 mL . L-1 mostrou-se o menos tóxico, pois não afetou o desenvolvimento vegetativo e o enraizamento in vitro de plântulas de Oncidium varicosum.
abstract_unstemmed	One of the greatest problem of in vitro production of seedling is the contamination of the culture medium during the micropropagation stages. This study was carried out to investigate the effect of fungicides and germicide on the plant growth and rooting, degree of phytotoxicity and control of contamination during in vitro cultivation of O. varicosum orchid. The flowers were pollinated artificially and the capsules seeds were sowed in MS culture medium modified with half of the macronutrients. The seedlings were transferred to the same culture medium uded for germination, added with the fungicides azoxystrobin, benomyl, chlorothalonil ( 0 g.L-1 , 0.1 g.L-1 ; 0.2 g.L-1 ; 0.4 g.L-1 ; 0.6 g.L-1 and 0.8 g.L-1) or sodium hypochloride with 10% active chloride ( 0 mL. L-1 , 0,5 mL.L-1 ; 0,75 mL. L-1 ; 1,00 mL. L-1 ; 1,25 mL. L-1 ; 1,50 mL. L-1). A complete randomized block design was used with ten replications per treatment and five plantlets per flask. The treatment containing 1.5 mL L-1 sodium hypochloride was the best in preventing contamination by microorganisms and did not cause any apparent harm development and in vitro rooting of Oncidium varicosum plantlets.<p<<p<Um dos maiores problemas na produção de mudas in vitro, é a contaminação por fungos e bactérias do meio de cultura durante as etapas de micropropagação, mesmo havendo todos os cuidados de assepsia. O presente trabalho teve por objetivo avaliar o efeito de fungicidas e germicida no crescimento vegetativo e enraizamento in vitro da orquídea Oncidium varicosum Lindl. As flores foram polinizadas artificialmente e as sementes foram germinadas no meio MS modificado com a metade da concentração dos macronutrientes. As plântulas obtidas foram subcultivadas no mesmo meio de germinação, adicionando-se os fungicidas azoxystrobin, benomyl, chlorothalonil nas concentrações: 0,1 g.L-1; 0,2 g.L-1 ; 0,4 g.L-1; 0,6 g .L-1 e 0,8 g.L-1 O germicida hipoclorito de sódio com 10% de cloro ativo foi adicionado ao meio nutritivo nas concentrações: 0,5 mL. L-1; 0,75 mL .L-1 ; 1,00 mL .L-1 ; 1,25 mL. L-1 e 1,50 mL. L-1 . O delineamento experimental foi o inteiramente casualizado, com dez repetições por tratamento e cinco plântulas por frasco. O tratamento contendo hipoclorito de sódio 1,5 mL . L-1 mostrou-se o menos tóxico, pois não afetou o desenvolvimento vegetativo e o enraizamento in vitro de plântulas de Oncidium varicosum.
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title_short	Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) <br< Avaliação da fitotoxicidade de fungicidas e germicida na propagação in vitro de Oncidium varicosum Lindl. (Orchidaceae) para o controle de microorganismos
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(Orchidaceae) <br< Avaliação da fitotoxicidade de fungicidas e germicida na propagação in vitro de Oncidium varicosum Lindl. (Orchidaceae) para o controle de microorganismos</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2003</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">One of the greatest problem of in vitro production of seedling is the contamination of the culture medium during the micropropagation stages. This study was carried out to investigate the effect of fungicides and germicide on the plant growth and rooting, degree of phytotoxicity and control of contamination during in vitro cultivation of O. varicosum orchid. The flowers were pollinated artificially and the capsules seeds were sowed in MS culture medium modified with half of the macronutrients. The seedlings were transferred to the same culture medium uded for germination, added with the fungicides azoxystrobin, benomyl, chlorothalonil ( 0 g.L-1 , 0.1 g.L-1 ; 0.2 g.L-1 ; 0.4 g.L-1 ; 0.6 g.L-1 and 0.8 g.L-1) or sodium hypochloride with 10% active chloride ( 0 mL. L-1 , 0,5 mL.L-1 ; 0,75 mL. L-1 ; 1,00 mL. L-1 ; 1,25 mL. L-1 ; 1,50 mL. L-1). A complete randomized block design was used with ten replications per treatment and five plantlets per flask. The treatment containing 1.5 mL L-1 sodium hypochloride was the best in preventing contamination by microorganisms and did not cause any apparent harm development and in vitro rooting of Oncidium varicosum plantlets.<p<<p<Um dos maiores problemas na produção de mudas in vitro, é a contaminação por fungos e bactérias do meio de cultura durante as etapas de micropropagação, mesmo havendo todos os cuidados de assepsia. O presente trabalho teve por objetivo avaliar o efeito de fungicidas e germicida no crescimento vegetativo e enraizamento in vitro da orquídea Oncidium varicosum Lindl. As flores foram polinizadas artificialmente e as sementes foram germinadas no meio MS modificado com a metade da concentração dos macronutrientes. As plântulas obtidas foram subcultivadas no mesmo meio de germinação, adicionando-se os fungicidas azoxystrobin, benomyl, chlorothalonil nas concentrações: 0,1 g.L-1; 0,2 g.L-1 ; 0,4 g.L-1; 0,6 g .L-1 e 0,8 g.L-1 O germicida hipoclorito de sódio com 10% de cloro ativo foi adicionado ao meio nutritivo nas concentrações: 0,5 mL. L-1; 0,75 mL .L-1 ; 1,00 mL .L-1 ; 1,25 mL. L-1 e 1,50 mL. L-1 . O delineamento experimental foi o inteiramente casualizado, com dez repetições por tratamento e cinco plântulas por frasco. O tratamento contendo hipoclorito de sódio 1,5 mL . L-1 mostrou-se o menos tóxico, pois não afetou o desenvolvimento vegetativo e o enraizamento in vitro de plântulas de Oncidium varicosum.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cultura de tecidos</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Meio de cultura</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Orquídea</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fungos</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Bactérias.</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Agriculture (General)</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Inês Cristina Batista Fonseca</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Ricardo Tadeu de 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Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) <br< Avaliação da fitotoxicidade de fungicidas e germicida na propagação in vitro de Oncidium varicosum Lindl. (Orchidaceae) para o controle de microorganismos

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