Diagnostic utility of Wilms′ tumour-1 protein (WT-1) immunostaining in paediatric renal tumours
Background & objectives: Renal tumours constitute about 7 per cent of all neoplasms in children. It is important to differentiate Wilms′ tumour (commonest tumour) from non-Wilms′ tumours. The aim of this study was to evaluate the immunoexpression and diagnostic role of Wilms′ tumour-1 protein (W...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Surbhi Goyal [verfasserIn] Kiran Mishra [verfasserIn] Urvee Sarkar [verfasserIn] Satendra Sharma [verfasserIn] Anita Kumari [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2016 |
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| Schlagwörter: |
Immunostaining - non-Wilms′ - paediatric renal tumours - Wilms′ tumour - WT1 |
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| Übergeordnetes Werk: |
In: Indian Journal of Medical Research - Wolters Kluwer Medknow Publications, 2005, 143(2016), 7, Seite 59-67 |
|---|---|
| Übergeordnetes Werk: |
volume:143 ; year:2016 ; number:7 ; pages:59-67 |
| Links: |
|---|
| DOI / URN: |
10.4103/0971-5916.191776 |
|---|
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DOAJ013286404 |
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| 520 | |a Background & objectives: Renal tumours constitute about 7 per cent of all neoplasms in children. It is important to differentiate Wilms′ tumour (commonest tumour) from non-Wilms′ tumours. The aim of this study was to evaluate the immunoexpression and diagnostic role of Wilms′ tumour-1 protein (WT1) in paediatric renal tumours. Methods: A total of 53 cases of renal tumours in children (below 18 yr) who underwent total nephrectomy were included in this retrospective study. WT1 immunostaining was done using mouse monoclonal WT1 antibody (clone: 6F-H2). Results: Of the 53 cases, 38 (72%) were of Wilms′ tumour. Non-Wilms′ group (15) included six cases of mesoblastic nephroma (MN), two each of clear cell sarcoma (CCSK), renal cell carcinoma (RCC) and peripheral neuroectodermal tumour (PNET) and one each of angiomyolipoma (AML), rhabdomyosarcoma (RMS) and malignant rhabdoid tumour (MRT). Proportion of WT1 positivity in Wilms′ tumour was 100 per cent in contrast to 26.7 per cent in non-Wilms′ tumours ( P<0.001). Epithelial and blastemal components of Wilms′ tumour showed moderate (2+) nuclear and cytoplasmic staining in 80 (24/30) and 75 per cent (24/32) cases, respectively. MN, PNET, CCSK and AML were negative for WT1. RMS, RCC and MRT showed cytoplasmic staining, strongest in RMS. No significant association was seen between WT1 expression and NWTSG (National Wilms′ Tumor Study Group) stage. Interpretation & conclusions: WT1 helps to differentiate Wilms′ tumour from other paediatric renal tumours. It may help in differentiating the two subgroups of Wilms′ tumour which have distinct molecular pathogenesis and biological behaviour, however, further prospective studies are required for validation of this hypothesis. | ||
| 650 | 4 | |a Immunostaining - non-Wilms′ - paediatric renal tumours - Wilms′ tumour - WT1 | |
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10.4103/0971-5916.191776 doi (DE-627)DOAJ013286404 (DE-599)DOAJ2b490c7587e84d5db97384af0c2b052d DE-627 ger DE-627 rakwb eng Surbhi Goyal verfasserin aut Diagnostic utility of Wilms′ tumour-1 protein (WT-1) immunostaining in paediatric renal tumours 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background & objectives: Renal tumours constitute about 7 per cent of all neoplasms in children. It is important to differentiate Wilms′ tumour (commonest tumour) from non-Wilms′ tumours. The aim of this study was to evaluate the immunoexpression and diagnostic role of Wilms′ tumour-1 protein (WT1) in paediatric renal tumours. Methods: A total of 53 cases of renal tumours in children (below 18 yr) who underwent total nephrectomy were included in this retrospective study. WT1 immunostaining was done using mouse monoclonal WT1 antibody (clone: 6F-H2). Results: Of the 53 cases, 38 (72%) were of Wilms′ tumour. Non-Wilms′ group (15) included six cases of mesoblastic nephroma (MN), two each of clear cell sarcoma (CCSK), renal cell carcinoma (RCC) and peripheral neuroectodermal tumour (PNET) and one each of angiomyolipoma (AML), rhabdomyosarcoma (RMS) and malignant rhabdoid tumour (MRT). Proportion of WT1 positivity in Wilms′ tumour was 100 per cent in contrast to 26.7 per cent in non-Wilms′ tumours ( P<0.001). Epithelial and blastemal components of Wilms′ tumour showed moderate (2+) nuclear and cytoplasmic staining in 80 (24/30) and 75 per cent (24/32) cases, respectively. MN, PNET, CCSK and AML were negative for WT1. RMS, RCC and MRT showed cytoplasmic staining, strongest in RMS. No significant association was seen between WT1 expression and NWTSG (National Wilms′ Tumor Study Group) stage. Interpretation & conclusions: WT1 helps to differentiate Wilms′ tumour from other paediatric renal tumours. It may help in differentiating the two subgroups of Wilms′ tumour which have distinct molecular pathogenesis and biological behaviour, however, further prospective studies are required for validation of this hypothesis. Immunostaining - non-Wilms′ - paediatric renal tumours - Wilms′ tumour - WT1 Medicine R Kiran Mishra verfasserin aut Urvee Sarkar verfasserin aut Satendra Sharma verfasserin aut Anita Kumari verfasserin aut In Indian Journal of Medical Research Wolters Kluwer Medknow Publications, 2005 143(2016), 7, Seite 59-67 (DE-627)DOAJ000026956 09715916 nnns volume:143 year:2016 number:7 pages:59-67 https://doi.org/10.4103/0971-5916.191776 kostenfrei https://doaj.org/article/2b490c7587e84d5db97384af0c2b052d kostenfrei http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2016;volume=143;issue=7;spage=59;epage=67;aulast=Goyal kostenfrei https://doaj.org/toc/0971-5916 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 143 2016 7 59-67 |
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10.4103/0971-5916.191776 doi (DE-627)DOAJ013286404 (DE-599)DOAJ2b490c7587e84d5db97384af0c2b052d DE-627 ger DE-627 rakwb eng Surbhi Goyal verfasserin aut Diagnostic utility of Wilms′ tumour-1 protein (WT-1) immunostaining in paediatric renal tumours 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background & objectives: Renal tumours constitute about 7 per cent of all neoplasms in children. It is important to differentiate Wilms′ tumour (commonest tumour) from non-Wilms′ tumours. The aim of this study was to evaluate the immunoexpression and diagnostic role of Wilms′ tumour-1 protein (WT1) in paediatric renal tumours. Methods: A total of 53 cases of renal tumours in children (below 18 yr) who underwent total nephrectomy were included in this retrospective study. WT1 immunostaining was done using mouse monoclonal WT1 antibody (clone: 6F-H2). Results: Of the 53 cases, 38 (72%) were of Wilms′ tumour. Non-Wilms′ group (15) included six cases of mesoblastic nephroma (MN), two each of clear cell sarcoma (CCSK), renal cell carcinoma (RCC) and peripheral neuroectodermal tumour (PNET) and one each of angiomyolipoma (AML), rhabdomyosarcoma (RMS) and malignant rhabdoid tumour (MRT). Proportion of WT1 positivity in Wilms′ tumour was 100 per cent in contrast to 26.7 per cent in non-Wilms′ tumours ( P<0.001). Epithelial and blastemal components of Wilms′ tumour showed moderate (2+) nuclear and cytoplasmic staining in 80 (24/30) and 75 per cent (24/32) cases, respectively. MN, PNET, CCSK and AML were negative for WT1. RMS, RCC and MRT showed cytoplasmic staining, strongest in RMS. No significant association was seen between WT1 expression and NWTSG (National Wilms′ Tumor Study Group) stage. Interpretation & conclusions: WT1 helps to differentiate Wilms′ tumour from other paediatric renal tumours. It may help in differentiating the two subgroups of Wilms′ tumour which have distinct molecular pathogenesis and biological behaviour, however, further prospective studies are required for validation of this hypothesis. Immunostaining - non-Wilms′ - paediatric renal tumours - Wilms′ tumour - WT1 Medicine R Kiran Mishra verfasserin aut Urvee Sarkar verfasserin aut Satendra Sharma verfasserin aut Anita Kumari verfasserin aut In Indian Journal of Medical Research Wolters Kluwer Medknow Publications, 2005 143(2016), 7, Seite 59-67 (DE-627)DOAJ000026956 09715916 nnns volume:143 year:2016 number:7 pages:59-67 https://doi.org/10.4103/0971-5916.191776 kostenfrei https://doaj.org/article/2b490c7587e84d5db97384af0c2b052d kostenfrei http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2016;volume=143;issue=7;spage=59;epage=67;aulast=Goyal kostenfrei https://doaj.org/toc/0971-5916 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 143 2016 7 59-67 |
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10.4103/0971-5916.191776 doi (DE-627)DOAJ013286404 (DE-599)DOAJ2b490c7587e84d5db97384af0c2b052d DE-627 ger DE-627 rakwb eng Surbhi Goyal verfasserin aut Diagnostic utility of Wilms′ tumour-1 protein (WT-1) immunostaining in paediatric renal tumours 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background & objectives: Renal tumours constitute about 7 per cent of all neoplasms in children. It is important to differentiate Wilms′ tumour (commonest tumour) from non-Wilms′ tumours. The aim of this study was to evaluate the immunoexpression and diagnostic role of Wilms′ tumour-1 protein (WT1) in paediatric renal tumours. Methods: A total of 53 cases of renal tumours in children (below 18 yr) who underwent total nephrectomy were included in this retrospective study. WT1 immunostaining was done using mouse monoclonal WT1 antibody (clone: 6F-H2). Results: Of the 53 cases, 38 (72%) were of Wilms′ tumour. Non-Wilms′ group (15) included six cases of mesoblastic nephroma (MN), two each of clear cell sarcoma (CCSK), renal cell carcinoma (RCC) and peripheral neuroectodermal tumour (PNET) and one each of angiomyolipoma (AML), rhabdomyosarcoma (RMS) and malignant rhabdoid tumour (MRT). Proportion of WT1 positivity in Wilms′ tumour was 100 per cent in contrast to 26.7 per cent in non-Wilms′ tumours ( P<0.001). Epithelial and blastemal components of Wilms′ tumour showed moderate (2+) nuclear and cytoplasmic staining in 80 (24/30) and 75 per cent (24/32) cases, respectively. MN, PNET, CCSK and AML were negative for WT1. RMS, RCC and MRT showed cytoplasmic staining, strongest in RMS. No significant association was seen between WT1 expression and NWTSG (National Wilms′ Tumor Study Group) stage. Interpretation & conclusions: WT1 helps to differentiate Wilms′ tumour from other paediatric renal tumours. It may help in differentiating the two subgroups of Wilms′ tumour which have distinct molecular pathogenesis and biological behaviour, however, further prospective studies are required for validation of this hypothesis. Immunostaining - non-Wilms′ - paediatric renal tumours - Wilms′ tumour - WT1 Medicine R Kiran Mishra verfasserin aut Urvee Sarkar verfasserin aut Satendra Sharma verfasserin aut Anita Kumari verfasserin aut In Indian Journal of Medical Research Wolters Kluwer Medknow Publications, 2005 143(2016), 7, Seite 59-67 (DE-627)DOAJ000026956 09715916 nnns volume:143 year:2016 number:7 pages:59-67 https://doi.org/10.4103/0971-5916.191776 kostenfrei https://doaj.org/article/2b490c7587e84d5db97384af0c2b052d kostenfrei http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2016;volume=143;issue=7;spage=59;epage=67;aulast=Goyal kostenfrei https://doaj.org/toc/0971-5916 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 143 2016 7 59-67 |
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10.4103/0971-5916.191776 doi (DE-627)DOAJ013286404 (DE-599)DOAJ2b490c7587e84d5db97384af0c2b052d DE-627 ger DE-627 rakwb eng Surbhi Goyal verfasserin aut Diagnostic utility of Wilms′ tumour-1 protein (WT-1) immunostaining in paediatric renal tumours 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background & objectives: Renal tumours constitute about 7 per cent of all neoplasms in children. It is important to differentiate Wilms′ tumour (commonest tumour) from non-Wilms′ tumours. The aim of this study was to evaluate the immunoexpression and diagnostic role of Wilms′ tumour-1 protein (WT1) in paediatric renal tumours. Methods: A total of 53 cases of renal tumours in children (below 18 yr) who underwent total nephrectomy were included in this retrospective study. WT1 immunostaining was done using mouse monoclonal WT1 antibody (clone: 6F-H2). Results: Of the 53 cases, 38 (72%) were of Wilms′ tumour. Non-Wilms′ group (15) included six cases of mesoblastic nephroma (MN), two each of clear cell sarcoma (CCSK), renal cell carcinoma (RCC) and peripheral neuroectodermal tumour (PNET) and one each of angiomyolipoma (AML), rhabdomyosarcoma (RMS) and malignant rhabdoid tumour (MRT). Proportion of WT1 positivity in Wilms′ tumour was 100 per cent in contrast to 26.7 per cent in non-Wilms′ tumours ( P<0.001). Epithelial and blastemal components of Wilms′ tumour showed moderate (2+) nuclear and cytoplasmic staining in 80 (24/30) and 75 per cent (24/32) cases, respectively. MN, PNET, CCSK and AML were negative for WT1. RMS, RCC and MRT showed cytoplasmic staining, strongest in RMS. No significant association was seen between WT1 expression and NWTSG (National Wilms′ Tumor Study Group) stage. Interpretation & conclusions: WT1 helps to differentiate Wilms′ tumour from other paediatric renal tumours. It may help in differentiating the two subgroups of Wilms′ tumour which have distinct molecular pathogenesis and biological behaviour, however, further prospective studies are required for validation of this hypothesis. Immunostaining - non-Wilms′ - paediatric renal tumours - Wilms′ tumour - WT1 Medicine R Kiran Mishra verfasserin aut Urvee Sarkar verfasserin aut Satendra Sharma verfasserin aut Anita Kumari verfasserin aut In Indian Journal of Medical Research Wolters Kluwer Medknow Publications, 2005 143(2016), 7, Seite 59-67 (DE-627)DOAJ000026956 09715916 nnns volume:143 year:2016 number:7 pages:59-67 https://doi.org/10.4103/0971-5916.191776 kostenfrei https://doaj.org/article/2b490c7587e84d5db97384af0c2b052d kostenfrei http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2016;volume=143;issue=7;spage=59;epage=67;aulast=Goyal kostenfrei https://doaj.org/toc/0971-5916 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 143 2016 7 59-67 |
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10.4103/0971-5916.191776 doi (DE-627)DOAJ013286404 (DE-599)DOAJ2b490c7587e84d5db97384af0c2b052d DE-627 ger DE-627 rakwb eng Surbhi Goyal verfasserin aut Diagnostic utility of Wilms′ tumour-1 protein (WT-1) immunostaining in paediatric renal tumours 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background & objectives: Renal tumours constitute about 7 per cent of all neoplasms in children. It is important to differentiate Wilms′ tumour (commonest tumour) from non-Wilms′ tumours. The aim of this study was to evaluate the immunoexpression and diagnostic role of Wilms′ tumour-1 protein (WT1) in paediatric renal tumours. Methods: A total of 53 cases of renal tumours in children (below 18 yr) who underwent total nephrectomy were included in this retrospective study. WT1 immunostaining was done using mouse monoclonal WT1 antibody (clone: 6F-H2). Results: Of the 53 cases, 38 (72%) were of Wilms′ tumour. Non-Wilms′ group (15) included six cases of mesoblastic nephroma (MN), two each of clear cell sarcoma (CCSK), renal cell carcinoma (RCC) and peripheral neuroectodermal tumour (PNET) and one each of angiomyolipoma (AML), rhabdomyosarcoma (RMS) and malignant rhabdoid tumour (MRT). Proportion of WT1 positivity in Wilms′ tumour was 100 per cent in contrast to 26.7 per cent in non-Wilms′ tumours ( P<0.001). Epithelial and blastemal components of Wilms′ tumour showed moderate (2+) nuclear and cytoplasmic staining in 80 (24/30) and 75 per cent (24/32) cases, respectively. MN, PNET, CCSK and AML were negative for WT1. RMS, RCC and MRT showed cytoplasmic staining, strongest in RMS. No significant association was seen between WT1 expression and NWTSG (National Wilms′ Tumor Study Group) stage. Interpretation & conclusions: WT1 helps to differentiate Wilms′ tumour from other paediatric renal tumours. It may help in differentiating the two subgroups of Wilms′ tumour which have distinct molecular pathogenesis and biological behaviour, however, further prospective studies are required for validation of this hypothesis. Immunostaining - non-Wilms′ - paediatric renal tumours - Wilms′ tumour - WT1 Medicine R Kiran Mishra verfasserin aut Urvee Sarkar verfasserin aut Satendra Sharma verfasserin aut Anita Kumari verfasserin aut In Indian Journal of Medical Research Wolters Kluwer Medknow Publications, 2005 143(2016), 7, Seite 59-67 (DE-627)DOAJ000026956 09715916 nnns volume:143 year:2016 number:7 pages:59-67 https://doi.org/10.4103/0971-5916.191776 kostenfrei https://doaj.org/article/2b490c7587e84d5db97384af0c2b052d kostenfrei http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2016;volume=143;issue=7;spage=59;epage=67;aulast=Goyal kostenfrei https://doaj.org/toc/0971-5916 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 143 2016 7 59-67 |
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It is important to differentiate Wilms′ tumour (commonest tumour) from non-Wilms′ tumours. The aim of this study was to evaluate the immunoexpression and diagnostic role of Wilms′ tumour-1 protein (WT1) in paediatric renal tumours. Methods: A total of 53 cases of renal tumours in children (below 18 yr) who underwent total nephrectomy were included in this retrospective study. WT1 immunostaining was done using mouse monoclonal WT1 antibody (clone: 6F-H2). Results: Of the 53 cases, 38 (72%) were of Wilms′ tumour. Non-Wilms′ group (15) included six cases of mesoblastic nephroma (MN), two each of clear cell sarcoma (CCSK), renal cell carcinoma (RCC) and peripheral neuroectodermal tumour (PNET) and one each of angiomyolipoma (AML), rhabdomyosarcoma (RMS) and malignant rhabdoid tumour (MRT). Proportion of WT1 positivity in Wilms′ tumour was 100 per cent in contrast to 26.7 per cent in non-Wilms′ tumours ( P<0.001). Epithelial and blastemal components of Wilms′ tumour showed moderate (2+) nuclear and cytoplasmic staining in 80 (24/30) and 75 per cent (24/32) cases, respectively. MN, PNET, CCSK and AML were negative for WT1. RMS, RCC and MRT showed cytoplasmic staining, strongest in RMS. No significant association was seen between WT1 expression and NWTSG (National Wilms′ Tumor Study Group) stage. Interpretation & conclusions: WT1 helps to differentiate Wilms′ tumour from other paediatric renal tumours. 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Diagnostic utility of Wilms′ tumour-1 protein (WT-1) immunostaining in paediatric renal tumours |
| abstract |
Background & objectives: Renal tumours constitute about 7 per cent of all neoplasms in children. It is important to differentiate Wilms′ tumour (commonest tumour) from non-Wilms′ tumours. The aim of this study was to evaluate the immunoexpression and diagnostic role of Wilms′ tumour-1 protein (WT1) in paediatric renal tumours. Methods: A total of 53 cases of renal tumours in children (below 18 yr) who underwent total nephrectomy were included in this retrospective study. WT1 immunostaining was done using mouse monoclonal WT1 antibody (clone: 6F-H2). Results: Of the 53 cases, 38 (72%) were of Wilms′ tumour. Non-Wilms′ group (15) included six cases of mesoblastic nephroma (MN), two each of clear cell sarcoma (CCSK), renal cell carcinoma (RCC) and peripheral neuroectodermal tumour (PNET) and one each of angiomyolipoma (AML), rhabdomyosarcoma (RMS) and malignant rhabdoid tumour (MRT). Proportion of WT1 positivity in Wilms′ tumour was 100 per cent in contrast to 26.7 per cent in non-Wilms′ tumours ( P<0.001). Epithelial and blastemal components of Wilms′ tumour showed moderate (2+) nuclear and cytoplasmic staining in 80 (24/30) and 75 per cent (24/32) cases, respectively. MN, PNET, CCSK and AML were negative for WT1. RMS, RCC and MRT showed cytoplasmic staining, strongest in RMS. No significant association was seen between WT1 expression and NWTSG (National Wilms′ Tumor Study Group) stage. Interpretation & conclusions: WT1 helps to differentiate Wilms′ tumour from other paediatric renal tumours. It may help in differentiating the two subgroups of Wilms′ tumour which have distinct molecular pathogenesis and biological behaviour, however, further prospective studies are required for validation of this hypothesis. |
| abstractGer |
Background & objectives: Renal tumours constitute about 7 per cent of all neoplasms in children. It is important to differentiate Wilms′ tumour (commonest tumour) from non-Wilms′ tumours. The aim of this study was to evaluate the immunoexpression and diagnostic role of Wilms′ tumour-1 protein (WT1) in paediatric renal tumours. Methods: A total of 53 cases of renal tumours in children (below 18 yr) who underwent total nephrectomy were included in this retrospective study. WT1 immunostaining was done using mouse monoclonal WT1 antibody (clone: 6F-H2). Results: Of the 53 cases, 38 (72%) were of Wilms′ tumour. Non-Wilms′ group (15) included six cases of mesoblastic nephroma (MN), two each of clear cell sarcoma (CCSK), renal cell carcinoma (RCC) and peripheral neuroectodermal tumour (PNET) and one each of angiomyolipoma (AML), rhabdomyosarcoma (RMS) and malignant rhabdoid tumour (MRT). Proportion of WT1 positivity in Wilms′ tumour was 100 per cent in contrast to 26.7 per cent in non-Wilms′ tumours ( P<0.001). Epithelial and blastemal components of Wilms′ tumour showed moderate (2+) nuclear and cytoplasmic staining in 80 (24/30) and 75 per cent (24/32) cases, respectively. MN, PNET, CCSK and AML were negative for WT1. RMS, RCC and MRT showed cytoplasmic staining, strongest in RMS. No significant association was seen between WT1 expression and NWTSG (National Wilms′ Tumor Study Group) stage. Interpretation & conclusions: WT1 helps to differentiate Wilms′ tumour from other paediatric renal tumours. It may help in differentiating the two subgroups of Wilms′ tumour which have distinct molecular pathogenesis and biological behaviour, however, further prospective studies are required for validation of this hypothesis. |
| abstract_unstemmed |
Background & objectives: Renal tumours constitute about 7 per cent of all neoplasms in children. It is important to differentiate Wilms′ tumour (commonest tumour) from non-Wilms′ tumours. The aim of this study was to evaluate the immunoexpression and diagnostic role of Wilms′ tumour-1 protein (WT1) in paediatric renal tumours. Methods: A total of 53 cases of renal tumours in children (below 18 yr) who underwent total nephrectomy were included in this retrospective study. WT1 immunostaining was done using mouse monoclonal WT1 antibody (clone: 6F-H2). Results: Of the 53 cases, 38 (72%) were of Wilms′ tumour. Non-Wilms′ group (15) included six cases of mesoblastic nephroma (MN), two each of clear cell sarcoma (CCSK), renal cell carcinoma (RCC) and peripheral neuroectodermal tumour (PNET) and one each of angiomyolipoma (AML), rhabdomyosarcoma (RMS) and malignant rhabdoid tumour (MRT). Proportion of WT1 positivity in Wilms′ tumour was 100 per cent in contrast to 26.7 per cent in non-Wilms′ tumours ( P<0.001). Epithelial and blastemal components of Wilms′ tumour showed moderate (2+) nuclear and cytoplasmic staining in 80 (24/30) and 75 per cent (24/32) cases, respectively. MN, PNET, CCSK and AML were negative for WT1. RMS, RCC and MRT showed cytoplasmic staining, strongest in RMS. No significant association was seen between WT1 expression and NWTSG (National Wilms′ Tumor Study Group) stage. Interpretation & conclusions: WT1 helps to differentiate Wilms′ tumour from other paediatric renal tumours. It may help in differentiating the two subgroups of Wilms′ tumour which have distinct molecular pathogenesis and biological behaviour, however, further prospective studies are required for validation of this hypothesis. |
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