Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies

Duck viral enteritis is a highly contagious and fatal disease of commercial waterfowl flocks. The disease occurs sporadically or epizootically in mainland China due to insufficient vaccinations. Early and rapid diagnosis is important for preventive intervention and the control of epizootic events in...
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Autor*in:	Fengli Liu [verfasserIn] Yanxin Cao [verfasserIn] Maokai Yan [verfasserIn] Mengxu Sun [verfasserIn] Qingshui Zhang [verfasserIn] Jun Wang [verfasserIn] Guanghua Fu [verfasserIn] Rongchang Liu [verfasserIn] Yu Huang [verfasserIn] Jingliang Su [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	duck enteritis virus monoclonal antibody colloidal gold immunochromatographic assay strip

Übergeordnetes Werk:	In: Pathogens - MDPI AG, 2012, 10(2021), 3, p 365
Übergeordnetes Werk:	volume:10 ; year:2021 ; number:3, p 365

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/pathogens10030365

Katalog-ID:	DOAJ013304364

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allfields	10.3390/pathogens10030365 doi (DE-627)DOAJ013304364 (DE-599)DOAJc65d44c4f17341b29ac710682c080eb7 DE-627 ger DE-627 rakwb eng Fengli Liu verfasserin aut Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Duck viral enteritis is a highly contagious and fatal disease of commercial waterfowl flocks. The disease occurs sporadically or epizootically in mainland China due to insufficient vaccinations. Early and rapid diagnosis is important for preventive intervention and the control of epizootic events in clinical settings. In this study, we generated two monoclonal antibodies (MAbs) that specifically recognized the duck enteritis virus (DEV) envelope glycoprotein B and tegument protein UL47, respectively. Using these MAbs, a colloidal gold-based immunochromatographic assay (ICA) was developed for the efficient detection of DEV antigens within 15 min. Our results showed that the detection limit of the developed ICA strip was 2.52 × 10<sup<3</sup< TCID<sub<50</sub</mL for the virus infected cell culture suspension with no cross-reactivity with other pathogenic viruses commonly encountered in commercially raised waterfowl. Using samples from experimentally infected ducks, we demonstrated that the ICA detected the virus in cloacal swab samples on day three post-infection, demonstrating an 80% concordance with the PCR. For tissue homogenates from ducks succumbing to infection, the detection sensitivity was 100%. The efficient and specific detection by this ICA test provides a valuable, convenient, easy to use and rapid diagnostic tool for DVE under both laboratory and field conditions. duck enteritis virus monoclonal antibody colloidal gold immunochromatographic assay strip Medicine R Yanxin Cao verfasserin aut Maokai Yan verfasserin aut Mengxu Sun verfasserin aut Qingshui Zhang verfasserin aut Jun Wang verfasserin aut Guanghua Fu verfasserin aut Rongchang Liu verfasserin aut Yu Huang verfasserin aut Jingliang Su verfasserin aut In Pathogens MDPI AG, 2012 10(2021), 3, p 365 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:10 year:2021 number:3, p 365 https://doi.org/10.3390/pathogens10030365 kostenfrei https://doaj.org/article/c65d44c4f17341b29ac710682c080eb7 kostenfrei https://www.mdpi.com/2076-0817/10/3/365 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 3, p 365
spelling	10.3390/pathogens10030365 doi (DE-627)DOAJ013304364 (DE-599)DOAJc65d44c4f17341b29ac710682c080eb7 DE-627 ger DE-627 rakwb eng Fengli Liu verfasserin aut Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Duck viral enteritis is a highly contagious and fatal disease of commercial waterfowl flocks. The disease occurs sporadically or epizootically in mainland China due to insufficient vaccinations. Early and rapid diagnosis is important for preventive intervention and the control of epizootic events in clinical settings. In this study, we generated two monoclonal antibodies (MAbs) that specifically recognized the duck enteritis virus (DEV) envelope glycoprotein B and tegument protein UL47, respectively. Using these MAbs, a colloidal gold-based immunochromatographic assay (ICA) was developed for the efficient detection of DEV antigens within 15 min. Our results showed that the detection limit of the developed ICA strip was 2.52 × 10<sup<3</sup< TCID<sub<50</sub</mL for the virus infected cell culture suspension with no cross-reactivity with other pathogenic viruses commonly encountered in commercially raised waterfowl. Using samples from experimentally infected ducks, we demonstrated that the ICA detected the virus in cloacal swab samples on day three post-infection, demonstrating an 80% concordance with the PCR. For tissue homogenates from ducks succumbing to infection, the detection sensitivity was 100%. The efficient and specific detection by this ICA test provides a valuable, convenient, easy to use and rapid diagnostic tool for DVE under both laboratory and field conditions. duck enteritis virus monoclonal antibody colloidal gold immunochromatographic assay strip Medicine R Yanxin Cao verfasserin aut Maokai Yan verfasserin aut Mengxu Sun verfasserin aut Qingshui Zhang verfasserin aut Jun Wang verfasserin aut Guanghua Fu verfasserin aut Rongchang Liu verfasserin aut Yu Huang verfasserin aut Jingliang Su verfasserin aut In Pathogens MDPI AG, 2012 10(2021), 3, p 365 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:10 year:2021 number:3, p 365 https://doi.org/10.3390/pathogens10030365 kostenfrei https://doaj.org/article/c65d44c4f17341b29ac710682c080eb7 kostenfrei https://www.mdpi.com/2076-0817/10/3/365 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 3, p 365
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allfieldsGer	10.3390/pathogens10030365 doi (DE-627)DOAJ013304364 (DE-599)DOAJc65d44c4f17341b29ac710682c080eb7 DE-627 ger DE-627 rakwb eng Fengli Liu verfasserin aut Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Duck viral enteritis is a highly contagious and fatal disease of commercial waterfowl flocks. The disease occurs sporadically or epizootically in mainland China due to insufficient vaccinations. Early and rapid diagnosis is important for preventive intervention and the control of epizootic events in clinical settings. In this study, we generated two monoclonal antibodies (MAbs) that specifically recognized the duck enteritis virus (DEV) envelope glycoprotein B and tegument protein UL47, respectively. Using these MAbs, a colloidal gold-based immunochromatographic assay (ICA) was developed for the efficient detection of DEV antigens within 15 min. Our results showed that the detection limit of the developed ICA strip was 2.52 × 10<sup<3</sup< TCID<sub<50</sub</mL for the virus infected cell culture suspension with no cross-reactivity with other pathogenic viruses commonly encountered in commercially raised waterfowl. Using samples from experimentally infected ducks, we demonstrated that the ICA detected the virus in cloacal swab samples on day three post-infection, demonstrating an 80% concordance with the PCR. For tissue homogenates from ducks succumbing to infection, the detection sensitivity was 100%. The efficient and specific detection by this ICA test provides a valuable, convenient, easy to use and rapid diagnostic tool for DVE under both laboratory and field conditions. duck enteritis virus monoclonal antibody colloidal gold immunochromatographic assay strip Medicine R Yanxin Cao verfasserin aut Maokai Yan verfasserin aut Mengxu Sun verfasserin aut Qingshui Zhang verfasserin aut Jun Wang verfasserin aut Guanghua Fu verfasserin aut Rongchang Liu verfasserin aut Yu Huang verfasserin aut Jingliang Su verfasserin aut In Pathogens MDPI AG, 2012 10(2021), 3, p 365 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:10 year:2021 number:3, p 365 https://doi.org/10.3390/pathogens10030365 kostenfrei https://doaj.org/article/c65d44c4f17341b29ac710682c080eb7 kostenfrei https://www.mdpi.com/2076-0817/10/3/365 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 3, p 365
allfieldsSound	10.3390/pathogens10030365 doi (DE-627)DOAJ013304364 (DE-599)DOAJc65d44c4f17341b29ac710682c080eb7 DE-627 ger DE-627 rakwb eng Fengli Liu verfasserin aut Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Duck viral enteritis is a highly contagious and fatal disease of commercial waterfowl flocks. The disease occurs sporadically or epizootically in mainland China due to insufficient vaccinations. Early and rapid diagnosis is important for preventive intervention and the control of epizootic events in clinical settings. In this study, we generated two monoclonal antibodies (MAbs) that specifically recognized the duck enteritis virus (DEV) envelope glycoprotein B and tegument protein UL47, respectively. Using these MAbs, a colloidal gold-based immunochromatographic assay (ICA) was developed for the efficient detection of DEV antigens within 15 min. Our results showed that the detection limit of the developed ICA strip was 2.52 × 10<sup<3</sup< TCID<sub<50</sub</mL for the virus infected cell culture suspension with no cross-reactivity with other pathogenic viruses commonly encountered in commercially raised waterfowl. Using samples from experimentally infected ducks, we demonstrated that the ICA detected the virus in cloacal swab samples on day three post-infection, demonstrating an 80% concordance with the PCR. For tissue homogenates from ducks succumbing to infection, the detection sensitivity was 100%. The efficient and specific detection by this ICA test provides a valuable, convenient, easy to use and rapid diagnostic tool for DVE under both laboratory and field conditions. duck enteritis virus monoclonal antibody colloidal gold immunochromatographic assay strip Medicine R Yanxin Cao verfasserin aut Maokai Yan verfasserin aut Mengxu Sun verfasserin aut Qingshui Zhang verfasserin aut Jun Wang verfasserin aut Guanghua Fu verfasserin aut Rongchang Liu verfasserin aut Yu Huang verfasserin aut Jingliang Su verfasserin aut In Pathogens MDPI AG, 2012 10(2021), 3, p 365 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:10 year:2021 number:3, p 365 https://doi.org/10.3390/pathogens10030365 kostenfrei https://doaj.org/article/c65d44c4f17341b29ac710682c080eb7 kostenfrei https://www.mdpi.com/2076-0817/10/3/365 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 3, p 365
language	English
source	In Pathogens 10(2021), 3, p 365 volume:10 year:2021 number:3, p 365
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authorswithroles_txt_mv	Fengli Liu @@aut@@ Yanxin Cao @@aut@@ Maokai Yan @@aut@@ Mengxu Sun @@aut@@ Qingshui Zhang @@aut@@ Jun Wang @@aut@@ Guanghua Fu @@aut@@ Rongchang Liu @@aut@@ Yu Huang @@aut@@ Jingliang Su @@aut@@
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author	Fengli Liu
spellingShingle	Fengli Liu misc duck enteritis virus misc monoclonal antibody misc colloidal gold immunochromatographic assay strip misc Medicine misc R Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies
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topic_title	Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies duck enteritis virus monoclonal antibody colloidal gold immunochromatographic assay strip
topic	misc duck enteritis virus misc monoclonal antibody misc colloidal gold immunochromatographic assay strip misc Medicine misc R
topic_unstemmed	misc duck enteritis virus misc monoclonal antibody misc colloidal gold immunochromatographic assay strip misc Medicine misc R
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title	Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies
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title_full	Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies
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author_browse	Fengli Liu Yanxin Cao Maokai Yan Mengxu Sun Qingshui Zhang Jun Wang Guanghua Fu Rongchang Liu Yu Huang Jingliang Su
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title_sort	development of a colloidal gold immunochromatographic assay for duck enteritis virus detection using monoclonal antibodies
title_auth	Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies
abstract	Duck viral enteritis is a highly contagious and fatal disease of commercial waterfowl flocks. The disease occurs sporadically or epizootically in mainland China due to insufficient vaccinations. Early and rapid diagnosis is important for preventive intervention and the control of epizootic events in clinical settings. In this study, we generated two monoclonal antibodies (MAbs) that specifically recognized the duck enteritis virus (DEV) envelope glycoprotein B and tegument protein UL47, respectively. Using these MAbs, a colloidal gold-based immunochromatographic assay (ICA) was developed for the efficient detection of DEV antigens within 15 min. Our results showed that the detection limit of the developed ICA strip was 2.52 × 10<sup<3</sup< TCID<sub<50</sub</mL for the virus infected cell culture suspension with no cross-reactivity with other pathogenic viruses commonly encountered in commercially raised waterfowl. Using samples from experimentally infected ducks, we demonstrated that the ICA detected the virus in cloacal swab samples on day three post-infection, demonstrating an 80% concordance with the PCR. For tissue homogenates from ducks succumbing to infection, the detection sensitivity was 100%. The efficient and specific detection by this ICA test provides a valuable, convenient, easy to use and rapid diagnostic tool for DVE under both laboratory and field conditions.
abstractGer	Duck viral enteritis is a highly contagious and fatal disease of commercial waterfowl flocks. The disease occurs sporadically or epizootically in mainland China due to insufficient vaccinations. Early and rapid diagnosis is important for preventive intervention and the control of epizootic events in clinical settings. In this study, we generated two monoclonal antibodies (MAbs) that specifically recognized the duck enteritis virus (DEV) envelope glycoprotein B and tegument protein UL47, respectively. Using these MAbs, a colloidal gold-based immunochromatographic assay (ICA) was developed for the efficient detection of DEV antigens within 15 min. Our results showed that the detection limit of the developed ICA strip was 2.52 × 10<sup<3</sup< TCID<sub<50</sub</mL for the virus infected cell culture suspension with no cross-reactivity with other pathogenic viruses commonly encountered in commercially raised waterfowl. Using samples from experimentally infected ducks, we demonstrated that the ICA detected the virus in cloacal swab samples on day three post-infection, demonstrating an 80% concordance with the PCR. For tissue homogenates from ducks succumbing to infection, the detection sensitivity was 100%. The efficient and specific detection by this ICA test provides a valuable, convenient, easy to use and rapid diagnostic tool for DVE under both laboratory and field conditions.
abstract_unstemmed	Duck viral enteritis is a highly contagious and fatal disease of commercial waterfowl flocks. The disease occurs sporadically or epizootically in mainland China due to insufficient vaccinations. Early and rapid diagnosis is important for preventive intervention and the control of epizootic events in clinical settings. In this study, we generated two monoclonal antibodies (MAbs) that specifically recognized the duck enteritis virus (DEV) envelope glycoprotein B and tegument protein UL47, respectively. Using these MAbs, a colloidal gold-based immunochromatographic assay (ICA) was developed for the efficient detection of DEV antigens within 15 min. Our results showed that the detection limit of the developed ICA strip was 2.52 × 10<sup<3</sup< TCID<sub<50</sub</mL for the virus infected cell culture suspension with no cross-reactivity with other pathogenic viruses commonly encountered in commercially raised waterfowl. Using samples from experimentally infected ducks, we demonstrated that the ICA detected the virus in cloacal swab samples on day three post-infection, demonstrating an 80% concordance with the PCR. For tissue homogenates from ducks succumbing to infection, the detection sensitivity was 100%. The efficient and specific detection by this ICA test provides a valuable, convenient, easy to use and rapid diagnostic tool for DVE under both laboratory and field conditions.
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title_short	Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies
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up_date	2024-07-03T16:54:31.426Z
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Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies

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