A Novel Fluorescence Aptasensor Based on Magnetic Beads/Gold Nanoparticles/DNA-Stabilized Silver Nanoclusters for Detection of <i<Salmonella</i< Typhimurium

<i<Salmonella</i< Typhimurium (<i<S.</i< Typhimurium) is a globally distributed foodborne pathogen, which can lead to outbreaks of foodborne infectious diseases. It is essential to guarantee food safety by timely and correct detection of <i<S.</i< Typhimurium. In...
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Gespeichert in:

Autor*in:	Shiqian Fu [verfasserIn] Xinyan Yang [verfasserIn] Lidong Pang [verfasserIn] Shasha Cheng [verfasserIn] Danliangmin Song [verfasserIn] Xue Qin [verfasserIn] Chaoxin Man [verfasserIn] Yujun Jiang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	DNA-stabilized silver nanoclusters gold nanoparticles magnetic beads fluorescence aptasensor

Übergeordnetes Werk:	In: Foods - MDPI AG, 2013, 11(2022), 4, p 595
Übergeordnetes Werk:	volume:11 ; year:2022 ; number:4, p 595

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/foods11040595

Katalog-ID:	DOAJ013880845

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520			\|a <i<Salmonella</i< Typhimurium (<i<S.</i< Typhimurium) is a globally distributed foodborne pathogen, which can lead to outbreaks of foodborne infectious diseases. It is essential to guarantee food safety by timely and correct detection of <i<S.</i< Typhimurium. In this investigation, an original fluorescence aptasensor was constructed to detect <i<S.</i< Typhimurium rapidly and sensitively. Through the coupling of magnetic beads, aptamer, and gold nanoparticles (AuNPs), a fluorescence quenching system with a “sandwich structure” was established. The aptamer acted as a link, and its specific binding to <i<S.</i< Typhimurium could release AuNPs from the system. Meanwhile, fluorescent DNA-stabilized silver nanoclusters (DNA-AgNCs) were synthesized. The fluorescence intensity changes caused by the fluorescence resonance energy transfer between DNA-AgNCs and AuNPs were utilized to detect <i<S.</i< Typhimurium. The purposed aptasensor exhibited high selectivity and sensitivity with a linear response to <i<S.</i< Typhimurium, ranging from 3.7 × 10<sup<2</sup< to 3.7 × 10<sup<5</sup< cfu/mL. The limit of detection (LOD) was estimated to be 98 cfu/mL within 2 h 10 min. In addition, this method showed excellent application for detection of <i<S.</i< Typhimurium in artificially contaminated milk, with LOD reaching 3.4 × 10<sup<2</sup< cfu/mL. Therefore, the developed fluorescence aptasensor has great potential to identify <i<S.</i< Typhimurium in foodstuffs.
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allfields	10.3390/foods11040595 doi (DE-627)DOAJ013880845 (DE-599)DOAJ73c23cbe58d04096a715b70100788b9e DE-627 ger DE-627 rakwb eng TP1-1185 Shiqian Fu verfasserin aut A Novel Fluorescence Aptasensor Based on Magnetic Beads/Gold Nanoparticles/DNA-Stabilized Silver Nanoclusters for Detection of <i<Salmonella</i< Typhimurium 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Salmonella</i< Typhimurium (<i<S.</i< Typhimurium) is a globally distributed foodborne pathogen, which can lead to outbreaks of foodborne infectious diseases. It is essential to guarantee food safety by timely and correct detection of <i<S.</i< Typhimurium. In this investigation, an original fluorescence aptasensor was constructed to detect <i<S.</i< Typhimurium rapidly and sensitively. Through the coupling of magnetic beads, aptamer, and gold nanoparticles (AuNPs), a fluorescence quenching system with a “sandwich structure” was established. The aptamer acted as a link, and its specific binding to <i<S.</i< Typhimurium could release AuNPs from the system. Meanwhile, fluorescent DNA-stabilized silver nanoclusters (DNA-AgNCs) were synthesized. The fluorescence intensity changes caused by the fluorescence resonance energy transfer between DNA-AgNCs and AuNPs were utilized to detect <i<S.</i< Typhimurium. The purposed aptasensor exhibited high selectivity and sensitivity with a linear response to <i<S.</i< Typhimurium, ranging from 3.7 × 10<sup<2</sup< to 3.7 × 10<sup<5</sup< cfu/mL. The limit of detection (LOD) was estimated to be 98 cfu/mL within 2 h 10 min. In addition, this method showed excellent application for detection of <i<S.</i< Typhimurium in artificially contaminated milk, with LOD reaching 3.4 × 10<sup<2</sup< cfu/mL. Therefore, the developed fluorescence aptasensor has great potential to identify <i<S.</i< Typhimurium in foodstuffs. DNA-stabilized silver nanoclusters gold nanoparticles magnetic beads fluorescence aptasensor <i<Salmonella</i< Typhimurium Chemical technology Xinyan Yang verfasserin aut Lidong Pang verfasserin aut Shasha Cheng verfasserin aut Danliangmin Song verfasserin aut Xue Qin verfasserin aut Chaoxin Man verfasserin aut Yujun Jiang verfasserin aut In Foods MDPI AG, 2013 11(2022), 4, p 595 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:11 year:2022 number:4, p 595 https://doi.org/10.3390/foods11040595 kostenfrei https://doaj.org/article/73c23cbe58d04096a715b70100788b9e kostenfrei https://www.mdpi.com/2304-8158/11/4/595 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 4, p 595
spelling	10.3390/foods11040595 doi (DE-627)DOAJ013880845 (DE-599)DOAJ73c23cbe58d04096a715b70100788b9e DE-627 ger DE-627 rakwb eng TP1-1185 Shiqian Fu verfasserin aut A Novel Fluorescence Aptasensor Based on Magnetic Beads/Gold Nanoparticles/DNA-Stabilized Silver Nanoclusters for Detection of <i<Salmonella</i< Typhimurium 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Salmonella</i< Typhimurium (<i<S.</i< Typhimurium) is a globally distributed foodborne pathogen, which can lead to outbreaks of foodborne infectious diseases. It is essential to guarantee food safety by timely and correct detection of <i<S.</i< Typhimurium. In this investigation, an original fluorescence aptasensor was constructed to detect <i<S.</i< Typhimurium rapidly and sensitively. Through the coupling of magnetic beads, aptamer, and gold nanoparticles (AuNPs), a fluorescence quenching system with a “sandwich structure” was established. The aptamer acted as a link, and its specific binding to <i<S.</i< Typhimurium could release AuNPs from the system. Meanwhile, fluorescent DNA-stabilized silver nanoclusters (DNA-AgNCs) were synthesized. The fluorescence intensity changes caused by the fluorescence resonance energy transfer between DNA-AgNCs and AuNPs were utilized to detect <i<S.</i< Typhimurium. The purposed aptasensor exhibited high selectivity and sensitivity with a linear response to <i<S.</i< Typhimurium, ranging from 3.7 × 10<sup<2</sup< to 3.7 × 10<sup<5</sup< cfu/mL. The limit of detection (LOD) was estimated to be 98 cfu/mL within 2 h 10 min. In addition, this method showed excellent application for detection of <i<S.</i< Typhimurium in artificially contaminated milk, with LOD reaching 3.4 × 10<sup<2</sup< cfu/mL. Therefore, the developed fluorescence aptasensor has great potential to identify <i<S.</i< Typhimurium in foodstuffs. DNA-stabilized silver nanoclusters gold nanoparticles magnetic beads fluorescence aptasensor <i<Salmonella</i< Typhimurium Chemical technology Xinyan Yang verfasserin aut Lidong Pang verfasserin aut Shasha Cheng verfasserin aut Danliangmin Song verfasserin aut Xue Qin verfasserin aut Chaoxin Man verfasserin aut Yujun Jiang verfasserin aut In Foods MDPI AG, 2013 11(2022), 4, p 595 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:11 year:2022 number:4, p 595 https://doi.org/10.3390/foods11040595 kostenfrei https://doaj.org/article/73c23cbe58d04096a715b70100788b9e kostenfrei https://www.mdpi.com/2304-8158/11/4/595 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 4, p 595
allfields_unstemmed	10.3390/foods11040595 doi (DE-627)DOAJ013880845 (DE-599)DOAJ73c23cbe58d04096a715b70100788b9e DE-627 ger DE-627 rakwb eng TP1-1185 Shiqian Fu verfasserin aut A Novel Fluorescence Aptasensor Based on Magnetic Beads/Gold Nanoparticles/DNA-Stabilized Silver Nanoclusters for Detection of <i<Salmonella</i< Typhimurium 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Salmonella</i< Typhimurium (<i<S.</i< Typhimurium) is a globally distributed foodborne pathogen, which can lead to outbreaks of foodborne infectious diseases. It is essential to guarantee food safety by timely and correct detection of <i<S.</i< Typhimurium. In this investigation, an original fluorescence aptasensor was constructed to detect <i<S.</i< Typhimurium rapidly and sensitively. Through the coupling of magnetic beads, aptamer, and gold nanoparticles (AuNPs), a fluorescence quenching system with a “sandwich structure” was established. The aptamer acted as a link, and its specific binding to <i<S.</i< Typhimurium could release AuNPs from the system. Meanwhile, fluorescent DNA-stabilized silver nanoclusters (DNA-AgNCs) were synthesized. The fluorescence intensity changes caused by the fluorescence resonance energy transfer between DNA-AgNCs and AuNPs were utilized to detect <i<S.</i< Typhimurium. The purposed aptasensor exhibited high selectivity and sensitivity with a linear response to <i<S.</i< Typhimurium, ranging from 3.7 × 10<sup<2</sup< to 3.7 × 10<sup<5</sup< cfu/mL. The limit of detection (LOD) was estimated to be 98 cfu/mL within 2 h 10 min. In addition, this method showed excellent application for detection of <i<S.</i< Typhimurium in artificially contaminated milk, with LOD reaching 3.4 × 10<sup<2</sup< cfu/mL. Therefore, the developed fluorescence aptasensor has great potential to identify <i<S.</i< Typhimurium in foodstuffs. DNA-stabilized silver nanoclusters gold nanoparticles magnetic beads fluorescence aptasensor <i<Salmonella</i< Typhimurium Chemical technology Xinyan Yang verfasserin aut Lidong Pang verfasserin aut Shasha Cheng verfasserin aut Danliangmin Song verfasserin aut Xue Qin verfasserin aut Chaoxin Man verfasserin aut Yujun Jiang verfasserin aut In Foods MDPI AG, 2013 11(2022), 4, p 595 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:11 year:2022 number:4, p 595 https://doi.org/10.3390/foods11040595 kostenfrei https://doaj.org/article/73c23cbe58d04096a715b70100788b9e kostenfrei https://www.mdpi.com/2304-8158/11/4/595 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 4, p 595
allfieldsGer	10.3390/foods11040595 doi (DE-627)DOAJ013880845 (DE-599)DOAJ73c23cbe58d04096a715b70100788b9e DE-627 ger DE-627 rakwb eng TP1-1185 Shiqian Fu verfasserin aut A Novel Fluorescence Aptasensor Based on Magnetic Beads/Gold Nanoparticles/DNA-Stabilized Silver Nanoclusters for Detection of <i<Salmonella</i< Typhimurium 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Salmonella</i< Typhimurium (<i<S.</i< Typhimurium) is a globally distributed foodborne pathogen, which can lead to outbreaks of foodborne infectious diseases. It is essential to guarantee food safety by timely and correct detection of <i<S.</i< Typhimurium. In this investigation, an original fluorescence aptasensor was constructed to detect <i<S.</i< Typhimurium rapidly and sensitively. Through the coupling of magnetic beads, aptamer, and gold nanoparticles (AuNPs), a fluorescence quenching system with a “sandwich structure” was established. The aptamer acted as a link, and its specific binding to <i<S.</i< Typhimurium could release AuNPs from the system. Meanwhile, fluorescent DNA-stabilized silver nanoclusters (DNA-AgNCs) were synthesized. The fluorescence intensity changes caused by the fluorescence resonance energy transfer between DNA-AgNCs and AuNPs were utilized to detect <i<S.</i< Typhimurium. The purposed aptasensor exhibited high selectivity and sensitivity with a linear response to <i<S.</i< Typhimurium, ranging from 3.7 × 10<sup<2</sup< to 3.7 × 10<sup<5</sup< cfu/mL. The limit of detection (LOD) was estimated to be 98 cfu/mL within 2 h 10 min. In addition, this method showed excellent application for detection of <i<S.</i< Typhimurium in artificially contaminated milk, with LOD reaching 3.4 × 10<sup<2</sup< cfu/mL. Therefore, the developed fluorescence aptasensor has great potential to identify <i<S.</i< Typhimurium in foodstuffs. DNA-stabilized silver nanoclusters gold nanoparticles magnetic beads fluorescence aptasensor <i<Salmonella</i< Typhimurium Chemical technology Xinyan Yang verfasserin aut Lidong Pang verfasserin aut Shasha Cheng verfasserin aut Danliangmin Song verfasserin aut Xue Qin verfasserin aut Chaoxin Man verfasserin aut Yujun Jiang verfasserin aut In Foods MDPI AG, 2013 11(2022), 4, p 595 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:11 year:2022 number:4, p 595 https://doi.org/10.3390/foods11040595 kostenfrei https://doaj.org/article/73c23cbe58d04096a715b70100788b9e kostenfrei https://www.mdpi.com/2304-8158/11/4/595 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 4, p 595
allfieldsSound	10.3390/foods11040595 doi (DE-627)DOAJ013880845 (DE-599)DOAJ73c23cbe58d04096a715b70100788b9e DE-627 ger DE-627 rakwb eng TP1-1185 Shiqian Fu verfasserin aut A Novel Fluorescence Aptasensor Based on Magnetic Beads/Gold Nanoparticles/DNA-Stabilized Silver Nanoclusters for Detection of <i<Salmonella</i< Typhimurium 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Salmonella</i< Typhimurium (<i<S.</i< Typhimurium) is a globally distributed foodborne pathogen, which can lead to outbreaks of foodborne infectious diseases. It is essential to guarantee food safety by timely and correct detection of <i<S.</i< Typhimurium. In this investigation, an original fluorescence aptasensor was constructed to detect <i<S.</i< Typhimurium rapidly and sensitively. Through the coupling of magnetic beads, aptamer, and gold nanoparticles (AuNPs), a fluorescence quenching system with a “sandwich structure” was established. The aptamer acted as a link, and its specific binding to <i<S.</i< Typhimurium could release AuNPs from the system. Meanwhile, fluorescent DNA-stabilized silver nanoclusters (DNA-AgNCs) were synthesized. The fluorescence intensity changes caused by the fluorescence resonance energy transfer between DNA-AgNCs and AuNPs were utilized to detect <i<S.</i< Typhimurium. The purposed aptasensor exhibited high selectivity and sensitivity with a linear response to <i<S.</i< Typhimurium, ranging from 3.7 × 10<sup<2</sup< to 3.7 × 10<sup<5</sup< cfu/mL. The limit of detection (LOD) was estimated to be 98 cfu/mL within 2 h 10 min. In addition, this method showed excellent application for detection of <i<S.</i< Typhimurium in artificially contaminated milk, with LOD reaching 3.4 × 10<sup<2</sup< cfu/mL. Therefore, the developed fluorescence aptasensor has great potential to identify <i<S.</i< Typhimurium in foodstuffs. DNA-stabilized silver nanoclusters gold nanoparticles magnetic beads fluorescence aptasensor <i<Salmonella</i< Typhimurium Chemical technology Xinyan Yang verfasserin aut Lidong Pang verfasserin aut Shasha Cheng verfasserin aut Danliangmin Song verfasserin aut Xue Qin verfasserin aut Chaoxin Man verfasserin aut Yujun Jiang verfasserin aut In Foods MDPI AG, 2013 11(2022), 4, p 595 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:11 year:2022 number:4, p 595 https://doi.org/10.3390/foods11040595 kostenfrei https://doaj.org/article/73c23cbe58d04096a715b70100788b9e kostenfrei https://www.mdpi.com/2304-8158/11/4/595 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 4, p 595
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callnumber-first	T - Technology
author	Shiqian Fu
spellingShingle	Shiqian Fu misc TP1-1185 misc DNA-stabilized silver nanoclusters misc gold nanoparticles misc magnetic beads misc fluorescence aptasensor misc <i<Salmonella</i< Typhimurium misc Chemical technology A Novel Fluorescence Aptasensor Based on Magnetic Beads/Gold Nanoparticles/DNA-Stabilized Silver Nanoclusters for Detection of <i<Salmonella</i< Typhimurium
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topic_title	TP1-1185 A Novel Fluorescence Aptasensor Based on Magnetic Beads/Gold Nanoparticles/DNA-Stabilized Silver Nanoclusters for Detection of <i<Salmonella</i< Typhimurium DNA-stabilized silver nanoclusters gold nanoparticles magnetic beads fluorescence aptasensor <i<Salmonella</i< Typhimurium
topic	misc TP1-1185 misc DNA-stabilized silver nanoclusters misc gold nanoparticles misc magnetic beads misc fluorescence aptasensor misc <i<Salmonella</i< Typhimurium misc Chemical technology
topic_unstemmed	misc TP1-1185 misc DNA-stabilized silver nanoclusters misc gold nanoparticles misc magnetic beads misc fluorescence aptasensor misc <i<Salmonella</i< Typhimurium misc Chemical technology
topic_browse	misc TP1-1185 misc DNA-stabilized silver nanoclusters misc gold nanoparticles misc magnetic beads misc fluorescence aptasensor misc <i<Salmonella</i< Typhimurium misc Chemical technology
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title	A Novel Fluorescence Aptasensor Based on Magnetic Beads/Gold Nanoparticles/DNA-Stabilized Silver Nanoclusters for Detection of <i<Salmonella</i< Typhimurium
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title_full	A Novel Fluorescence Aptasensor Based on Magnetic Beads/Gold Nanoparticles/DNA-Stabilized Silver Nanoclusters for Detection of <i<Salmonella</i< Typhimurium
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title_sort	novel fluorescence aptasensor based on magnetic beads/gold nanoparticles/dna-stabilized silver nanoclusters for detection of <i<salmonella</i< typhimurium
callnumber	TP1-1185
title_auth	A Novel Fluorescence Aptasensor Based on Magnetic Beads/Gold Nanoparticles/DNA-Stabilized Silver Nanoclusters for Detection of <i<Salmonella</i< Typhimurium
abstract	<i<Salmonella</i< Typhimurium (<i<S.</i< Typhimurium) is a globally distributed foodborne pathogen, which can lead to outbreaks of foodborne infectious diseases. It is essential to guarantee food safety by timely and correct detection of <i<S.</i< Typhimurium. In this investigation, an original fluorescence aptasensor was constructed to detect <i<S.</i< Typhimurium rapidly and sensitively. Through the coupling of magnetic beads, aptamer, and gold nanoparticles (AuNPs), a fluorescence quenching system with a “sandwich structure” was established. The aptamer acted as a link, and its specific binding to <i<S.</i< Typhimurium could release AuNPs from the system. Meanwhile, fluorescent DNA-stabilized silver nanoclusters (DNA-AgNCs) were synthesized. The fluorescence intensity changes caused by the fluorescence resonance energy transfer between DNA-AgNCs and AuNPs were utilized to detect <i<S.</i< Typhimurium. The purposed aptasensor exhibited high selectivity and sensitivity with a linear response to <i<S.</i< Typhimurium, ranging from 3.7 × 10<sup<2</sup< to 3.7 × 10<sup<5</sup< cfu/mL. The limit of detection (LOD) was estimated to be 98 cfu/mL within 2 h 10 min. In addition, this method showed excellent application for detection of <i<S.</i< Typhimurium in artificially contaminated milk, with LOD reaching 3.4 × 10<sup<2</sup< cfu/mL. Therefore, the developed fluorescence aptasensor has great potential to identify <i<S.</i< Typhimurium in foodstuffs.
abstractGer	<i<Salmonella</i< Typhimurium (<i<S.</i< Typhimurium) is a globally distributed foodborne pathogen, which can lead to outbreaks of foodborne infectious diseases. It is essential to guarantee food safety by timely and correct detection of <i<S.</i< Typhimurium. In this investigation, an original fluorescence aptasensor was constructed to detect <i<S.</i< Typhimurium rapidly and sensitively. Through the coupling of magnetic beads, aptamer, and gold nanoparticles (AuNPs), a fluorescence quenching system with a “sandwich structure” was established. The aptamer acted as a link, and its specific binding to <i<S.</i< Typhimurium could release AuNPs from the system. Meanwhile, fluorescent DNA-stabilized silver nanoclusters (DNA-AgNCs) were synthesized. The fluorescence intensity changes caused by the fluorescence resonance energy transfer between DNA-AgNCs and AuNPs were utilized to detect <i<S.</i< Typhimurium. The purposed aptasensor exhibited high selectivity and sensitivity with a linear response to <i<S.</i< Typhimurium, ranging from 3.7 × 10<sup<2</sup< to 3.7 × 10<sup<5</sup< cfu/mL. The limit of detection (LOD) was estimated to be 98 cfu/mL within 2 h 10 min. In addition, this method showed excellent application for detection of <i<S.</i< Typhimurium in artificially contaminated milk, with LOD reaching 3.4 × 10<sup<2</sup< cfu/mL. Therefore, the developed fluorescence aptasensor has great potential to identify <i<S.</i< Typhimurium in foodstuffs.
abstract_unstemmed	<i<Salmonella</i< Typhimurium (<i<S.</i< Typhimurium) is a globally distributed foodborne pathogen, which can lead to outbreaks of foodborne infectious diseases. It is essential to guarantee food safety by timely and correct detection of <i<S.</i< Typhimurium. In this investigation, an original fluorescence aptasensor was constructed to detect <i<S.</i< Typhimurium rapidly and sensitively. Through the coupling of magnetic beads, aptamer, and gold nanoparticles (AuNPs), a fluorescence quenching system with a “sandwich structure” was established. The aptamer acted as a link, and its specific binding to <i<S.</i< Typhimurium could release AuNPs from the system. Meanwhile, fluorescent DNA-stabilized silver nanoclusters (DNA-AgNCs) were synthesized. The fluorescence intensity changes caused by the fluorescence resonance energy transfer between DNA-AgNCs and AuNPs were utilized to detect <i<S.</i< Typhimurium. The purposed aptasensor exhibited high selectivity and sensitivity with a linear response to <i<S.</i< Typhimurium, ranging from 3.7 × 10<sup<2</sup< to 3.7 × 10<sup<5</sup< cfu/mL. The limit of detection (LOD) was estimated to be 98 cfu/mL within 2 h 10 min. In addition, this method showed excellent application for detection of <i<S.</i< Typhimurium in artificially contaminated milk, with LOD reaching 3.4 × 10<sup<2</sup< cfu/mL. Therefore, the developed fluorescence aptasensor has great potential to identify <i<S.</i< Typhimurium in foodstuffs.
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container_issue	4, p 595
title_short	A Novel Fluorescence Aptasensor Based on Magnetic Beads/Gold Nanoparticles/DNA-Stabilized Silver Nanoclusters for Detection of <i<Salmonella</i< Typhimurium
url	https://doi.org/10.3390/foods11040595 https://doaj.org/article/73c23cbe58d04096a715b70100788b9e https://www.mdpi.com/2304-8158/11/4/595 https://doaj.org/toc/2304-8158
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It is essential to guarantee food safety by timely and correct detection of <i<S.</i< Typhimurium. In this investigation, an original fluorescence aptasensor was constructed to detect <i<S.</i< Typhimurium rapidly and sensitively. Through the coupling of magnetic beads, aptamer, and gold nanoparticles (AuNPs), a fluorescence quenching system with a “sandwich structure” was established. The aptamer acted as a link, and its specific binding to <i<S.</i< Typhimurium could release AuNPs from the system. Meanwhile, fluorescent DNA-stabilized silver nanoclusters (DNA-AgNCs) were synthesized. The fluorescence intensity changes caused by the fluorescence resonance energy transfer between DNA-AgNCs and AuNPs were utilized to detect <i<S.</i< Typhimurium. The purposed aptasensor exhibited high selectivity and sensitivity with a linear response to <i<S.</i< Typhimurium, ranging from 3.7 × 10<sup<2</sup< to 3.7 × 10<sup<5</sup< cfu/mL. The limit of detection (LOD) was estimated to be 98 cfu/mL within 2 h 10 min. In addition, this method showed excellent application for detection of <i<S.</i< Typhimurium in artificially contaminated milk, with LOD reaching 3.4 × 10<sup<2</sup< cfu/mL. 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A Novel Fluorescence Aptasensor Based on Magnetic Beads/Gold Nanoparticles/DNA-Stabilized Silver Nanoclusters for Detection of <i<Salmonella</i< Typhimurium

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