Serum response factor promotes differentiation of rat bone marrow mesenchymal stem cells into smooth muscle cells via histone acetylation
Objective To explore the role and mechanism of serum response factor (SRF) in differentiation of rat bone marrow mesenchymal stem cells (MSCs) into bladder smooth muscle cells (SMCs). Methods Rat MSCs and SMCs were isolated respectively from the bone marrow and bladder of 4-week-old female SD rats b...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
LIU Jingxia [verfasserIn] LIANG Zhiqing [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Chinesisch |
| Erschienen: |
2021 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Di-san junyi daxue xuebao - Editorial Office of Journal of Third Military Medical University, 2021, 43(2021), 4, Seite 303-310 |
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| Übergeordnetes Werk: |
volume:43 ; year:2021 ; number:4 ; pages:303-310 |
| Links: |
|---|
| DOI / URN: |
10.16016/j.1000-5404.202008213 |
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DOAJ01389207X |
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| 520 | |a Objective To explore the role and mechanism of serum response factor (SRF) in differentiation of rat bone marrow mesenchymal stem cells (MSCs) into bladder smooth muscle cells (SMCs). Methods Rat MSCs and SMCs were isolated respectively from the bone marrow and bladder of 4-week-old female SD rats by adherent method, and then co-cultured together in a Transwell chamber. After the MSCs were harvested at different time points, real-time PCR and immunofluorescense assay were used to detect the expression of SMCs specific marker genes in co-cultured MSCs. Furthermore, the enrichment of histone acetylation modification, histone deacetylase (HDAC) and SRF in the promoter of these genes were tested by chromatin immunoprecipitation assay (ChIP) q-PCR in co-cultured MSCs. Finally, ChIP-qPCR was used to compare the histone acetylation modification and SRF enrichment in sodium butyrate (NaB) pretreatment group with control cells. Results The expression levels of α-SMA, Calponin and SM-MHC were increased in MSCs co-cultured with SMCs. H3K9ace, H3ace and H4ace in the promoter of these genes were enriched (P<0.05), and then, HDAC1 and HDAC2 in the induced MSCs were correspondingly decreased significantly (P<0.05). The enrichment of SRF in the NaB pretreatment group shared the same increasing tendency with H3K9ace and H4ace. Conclusion SRF may bind to H3K9ace and H4ace, through the regulation of HDAC1 and HDAC2 to control the expression of SMCs specific genes, and thus promote the differentiation from MSCs to SMCs. | ||
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10.16016/j.1000-5404.202008213 doi (DE-627)DOAJ01389207X (DE-599)DOAJ89101c8892b847c8902c471dbd37b766 DE-627 ger DE-627 rakwb chi R5-920 LIU Jingxia verfasserin aut Serum response factor promotes differentiation of rat bone marrow mesenchymal stem cells into smooth muscle cells via histone acetylation 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To explore the role and mechanism of serum response factor (SRF) in differentiation of rat bone marrow mesenchymal stem cells (MSCs) into bladder smooth muscle cells (SMCs). Methods Rat MSCs and SMCs were isolated respectively from the bone marrow and bladder of 4-week-old female SD rats by adherent method, and then co-cultured together in a Transwell chamber. After the MSCs were harvested at different time points, real-time PCR and immunofluorescense assay were used to detect the expression of SMCs specific marker genes in co-cultured MSCs. Furthermore, the enrichment of histone acetylation modification, histone deacetylase (HDAC) and SRF in the promoter of these genes were tested by chromatin immunoprecipitation assay (ChIP) q-PCR in co-cultured MSCs. Finally, ChIP-qPCR was used to compare the histone acetylation modification and SRF enrichment in sodium butyrate (NaB) pretreatment group with control cells. Results The expression levels of α-SMA, Calponin and SM-MHC were increased in MSCs co-cultured with SMCs. H3K9ace, H3ace and H4ace in the promoter of these genes were enriched (P<0.05), and then, HDAC1 and HDAC2 in the induced MSCs were correspondingly decreased significantly (P<0.05). The enrichment of SRF in the NaB pretreatment group shared the same increasing tendency with H3K9ace and H4ace. Conclusion SRF may bind to H3K9ace and H4ace, through the regulation of HDAC1 and HDAC2 to control the expression of SMCs specific genes, and thus promote the differentiation from MSCs to SMCs. bone mesenchymal stem cells serum response factor differentiation histone acetylation sodium butyrate Medicine (General) LIANG Zhiqing verfasserin aut In Di-san junyi daxue xuebao Editorial Office of Journal of Third Military Medical University, 2021 43(2021), 4, Seite 303-310 (DE-627)1760645346 10005404 nnns volume:43 year:2021 number:4 pages:303-310 https://doi.org/10.16016/j.1000-5404.202008213 kostenfrei https://doaj.org/article/89101c8892b847c8902c471dbd37b766 kostenfrei https://aammt.tmmu.edu.cn/Upload/rhtml/202008213.htm kostenfrei https://doaj.org/toc/1000-5404 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 43 2021 4 303-310 |
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10.16016/j.1000-5404.202008213 doi (DE-627)DOAJ01389207X (DE-599)DOAJ89101c8892b847c8902c471dbd37b766 DE-627 ger DE-627 rakwb chi R5-920 LIU Jingxia verfasserin aut Serum response factor promotes differentiation of rat bone marrow mesenchymal stem cells into smooth muscle cells via histone acetylation 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To explore the role and mechanism of serum response factor (SRF) in differentiation of rat bone marrow mesenchymal stem cells (MSCs) into bladder smooth muscle cells (SMCs). Methods Rat MSCs and SMCs were isolated respectively from the bone marrow and bladder of 4-week-old female SD rats by adherent method, and then co-cultured together in a Transwell chamber. After the MSCs were harvested at different time points, real-time PCR and immunofluorescense assay were used to detect the expression of SMCs specific marker genes in co-cultured MSCs. Furthermore, the enrichment of histone acetylation modification, histone deacetylase (HDAC) and SRF in the promoter of these genes were tested by chromatin immunoprecipitation assay (ChIP) q-PCR in co-cultured MSCs. Finally, ChIP-qPCR was used to compare the histone acetylation modification and SRF enrichment in sodium butyrate (NaB) pretreatment group with control cells. Results The expression levels of α-SMA, Calponin and SM-MHC were increased in MSCs co-cultured with SMCs. H3K9ace, H3ace and H4ace in the promoter of these genes were enriched (P<0.05), and then, HDAC1 and HDAC2 in the induced MSCs were correspondingly decreased significantly (P<0.05). The enrichment of SRF in the NaB pretreatment group shared the same increasing tendency with H3K9ace and H4ace. Conclusion SRF may bind to H3K9ace and H4ace, through the regulation of HDAC1 and HDAC2 to control the expression of SMCs specific genes, and thus promote the differentiation from MSCs to SMCs. bone mesenchymal stem cells serum response factor differentiation histone acetylation sodium butyrate Medicine (General) LIANG Zhiqing verfasserin aut In Di-san junyi daxue xuebao Editorial Office of Journal of Third Military Medical University, 2021 43(2021), 4, Seite 303-310 (DE-627)1760645346 10005404 nnns volume:43 year:2021 number:4 pages:303-310 https://doi.org/10.16016/j.1000-5404.202008213 kostenfrei https://doaj.org/article/89101c8892b847c8902c471dbd37b766 kostenfrei https://aammt.tmmu.edu.cn/Upload/rhtml/202008213.htm kostenfrei https://doaj.org/toc/1000-5404 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 43 2021 4 303-310 |
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10.16016/j.1000-5404.202008213 doi (DE-627)DOAJ01389207X (DE-599)DOAJ89101c8892b847c8902c471dbd37b766 DE-627 ger DE-627 rakwb chi R5-920 LIU Jingxia verfasserin aut Serum response factor promotes differentiation of rat bone marrow mesenchymal stem cells into smooth muscle cells via histone acetylation 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To explore the role and mechanism of serum response factor (SRF) in differentiation of rat bone marrow mesenchymal stem cells (MSCs) into bladder smooth muscle cells (SMCs). Methods Rat MSCs and SMCs were isolated respectively from the bone marrow and bladder of 4-week-old female SD rats by adherent method, and then co-cultured together in a Transwell chamber. After the MSCs were harvested at different time points, real-time PCR and immunofluorescense assay were used to detect the expression of SMCs specific marker genes in co-cultured MSCs. Furthermore, the enrichment of histone acetylation modification, histone deacetylase (HDAC) and SRF in the promoter of these genes were tested by chromatin immunoprecipitation assay (ChIP) q-PCR in co-cultured MSCs. Finally, ChIP-qPCR was used to compare the histone acetylation modification and SRF enrichment in sodium butyrate (NaB) pretreatment group with control cells. Results The expression levels of α-SMA, Calponin and SM-MHC were increased in MSCs co-cultured with SMCs. H3K9ace, H3ace and H4ace in the promoter of these genes were enriched (P<0.05), and then, HDAC1 and HDAC2 in the induced MSCs were correspondingly decreased significantly (P<0.05). The enrichment of SRF in the NaB pretreatment group shared the same increasing tendency with H3K9ace and H4ace. Conclusion SRF may bind to H3K9ace and H4ace, through the regulation of HDAC1 and HDAC2 to control the expression of SMCs specific genes, and thus promote the differentiation from MSCs to SMCs. bone mesenchymal stem cells serum response factor differentiation histone acetylation sodium butyrate Medicine (General) LIANG Zhiqing verfasserin aut In Di-san junyi daxue xuebao Editorial Office of Journal of Third Military Medical University, 2021 43(2021), 4, Seite 303-310 (DE-627)1760645346 10005404 nnns volume:43 year:2021 number:4 pages:303-310 https://doi.org/10.16016/j.1000-5404.202008213 kostenfrei https://doaj.org/article/89101c8892b847c8902c471dbd37b766 kostenfrei https://aammt.tmmu.edu.cn/Upload/rhtml/202008213.htm kostenfrei https://doaj.org/toc/1000-5404 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 43 2021 4 303-310 |
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10.16016/j.1000-5404.202008213 doi (DE-627)DOAJ01389207X (DE-599)DOAJ89101c8892b847c8902c471dbd37b766 DE-627 ger DE-627 rakwb chi R5-920 LIU Jingxia verfasserin aut Serum response factor promotes differentiation of rat bone marrow mesenchymal stem cells into smooth muscle cells via histone acetylation 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To explore the role and mechanism of serum response factor (SRF) in differentiation of rat bone marrow mesenchymal stem cells (MSCs) into bladder smooth muscle cells (SMCs). Methods Rat MSCs and SMCs were isolated respectively from the bone marrow and bladder of 4-week-old female SD rats by adherent method, and then co-cultured together in a Transwell chamber. After the MSCs were harvested at different time points, real-time PCR and immunofluorescense assay were used to detect the expression of SMCs specific marker genes in co-cultured MSCs. Furthermore, the enrichment of histone acetylation modification, histone deacetylase (HDAC) and SRF in the promoter of these genes were tested by chromatin immunoprecipitation assay (ChIP) q-PCR in co-cultured MSCs. Finally, ChIP-qPCR was used to compare the histone acetylation modification and SRF enrichment in sodium butyrate (NaB) pretreatment group with control cells. Results The expression levels of α-SMA, Calponin and SM-MHC were increased in MSCs co-cultured with SMCs. H3K9ace, H3ace and H4ace in the promoter of these genes were enriched (P<0.05), and then, HDAC1 and HDAC2 in the induced MSCs were correspondingly decreased significantly (P<0.05). The enrichment of SRF in the NaB pretreatment group shared the same increasing tendency with H3K9ace and H4ace. Conclusion SRF may bind to H3K9ace and H4ace, through the regulation of HDAC1 and HDAC2 to control the expression of SMCs specific genes, and thus promote the differentiation from MSCs to SMCs. bone mesenchymal stem cells serum response factor differentiation histone acetylation sodium butyrate Medicine (General) LIANG Zhiqing verfasserin aut In Di-san junyi daxue xuebao Editorial Office of Journal of Third Military Medical University, 2021 43(2021), 4, Seite 303-310 (DE-627)1760645346 10005404 nnns volume:43 year:2021 number:4 pages:303-310 https://doi.org/10.16016/j.1000-5404.202008213 kostenfrei https://doaj.org/article/89101c8892b847c8902c471dbd37b766 kostenfrei https://aammt.tmmu.edu.cn/Upload/rhtml/202008213.htm kostenfrei https://doaj.org/toc/1000-5404 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 43 2021 4 303-310 |
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10.16016/j.1000-5404.202008213 doi (DE-627)DOAJ01389207X (DE-599)DOAJ89101c8892b847c8902c471dbd37b766 DE-627 ger DE-627 rakwb chi R5-920 LIU Jingxia verfasserin aut Serum response factor promotes differentiation of rat bone marrow mesenchymal stem cells into smooth muscle cells via histone acetylation 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To explore the role and mechanism of serum response factor (SRF) in differentiation of rat bone marrow mesenchymal stem cells (MSCs) into bladder smooth muscle cells (SMCs). Methods Rat MSCs and SMCs were isolated respectively from the bone marrow and bladder of 4-week-old female SD rats by adherent method, and then co-cultured together in a Transwell chamber. After the MSCs were harvested at different time points, real-time PCR and immunofluorescense assay were used to detect the expression of SMCs specific marker genes in co-cultured MSCs. Furthermore, the enrichment of histone acetylation modification, histone deacetylase (HDAC) and SRF in the promoter of these genes were tested by chromatin immunoprecipitation assay (ChIP) q-PCR in co-cultured MSCs. Finally, ChIP-qPCR was used to compare the histone acetylation modification and SRF enrichment in sodium butyrate (NaB) pretreatment group with control cells. Results The expression levels of α-SMA, Calponin and SM-MHC were increased in MSCs co-cultured with SMCs. H3K9ace, H3ace and H4ace in the promoter of these genes were enriched (P<0.05), and then, HDAC1 and HDAC2 in the induced MSCs were correspondingly decreased significantly (P<0.05). The enrichment of SRF in the NaB pretreatment group shared the same increasing tendency with H3K9ace and H4ace. Conclusion SRF may bind to H3K9ace and H4ace, through the regulation of HDAC1 and HDAC2 to control the expression of SMCs specific genes, and thus promote the differentiation from MSCs to SMCs. bone mesenchymal stem cells serum response factor differentiation histone acetylation sodium butyrate Medicine (General) LIANG Zhiqing verfasserin aut In Di-san junyi daxue xuebao Editorial Office of Journal of Third Military Medical University, 2021 43(2021), 4, Seite 303-310 (DE-627)1760645346 10005404 nnns volume:43 year:2021 number:4 pages:303-310 https://doi.org/10.16016/j.1000-5404.202008213 kostenfrei https://doaj.org/article/89101c8892b847c8902c471dbd37b766 kostenfrei https://aammt.tmmu.edu.cn/Upload/rhtml/202008213.htm kostenfrei https://doaj.org/toc/1000-5404 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 43 2021 4 303-310 |
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Methods Rat MSCs and SMCs were isolated respectively from the bone marrow and bladder of 4-week-old female SD rats by adherent method, and then co-cultured together in a Transwell chamber. After the MSCs were harvested at different time points, real-time PCR and immunofluorescense assay were used to detect the expression of SMCs specific marker genes in co-cultured MSCs. Furthermore, the enrichment of histone acetylation modification, histone deacetylase (HDAC) and SRF in the promoter of these genes were tested by chromatin immunoprecipitation assay (ChIP) q-PCR in co-cultured MSCs. Finally, ChIP-qPCR was used to compare the histone acetylation modification and SRF enrichment in sodium butyrate (NaB) pretreatment group with control cells. Results The expression levels of α-SMA, Calponin and SM-MHC were increased in MSCs co-cultured with SMCs. 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Serum response factor promotes differentiation of rat bone marrow mesenchymal stem cells into smooth muscle cells via histone acetylation |
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Serum response factor promotes differentiation of rat bone marrow mesenchymal stem cells into smooth muscle cells via histone acetylation |
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LIU Jingxia |
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Di-san junyi daxue xuebao |
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LIU Jingxia LIANG Zhiqing |
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LIU Jingxia |
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10.16016/j.1000-5404.202008213 |
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serum response factor promotes differentiation of rat bone marrow mesenchymal stem cells into smooth muscle cells via histone acetylation |
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Serum response factor promotes differentiation of rat bone marrow mesenchymal stem cells into smooth muscle cells via histone acetylation |
| abstract |
Objective To explore the role and mechanism of serum response factor (SRF) in differentiation of rat bone marrow mesenchymal stem cells (MSCs) into bladder smooth muscle cells (SMCs). Methods Rat MSCs and SMCs were isolated respectively from the bone marrow and bladder of 4-week-old female SD rats by adherent method, and then co-cultured together in a Transwell chamber. After the MSCs were harvested at different time points, real-time PCR and immunofluorescense assay were used to detect the expression of SMCs specific marker genes in co-cultured MSCs. Furthermore, the enrichment of histone acetylation modification, histone deacetylase (HDAC) and SRF in the promoter of these genes were tested by chromatin immunoprecipitation assay (ChIP) q-PCR in co-cultured MSCs. Finally, ChIP-qPCR was used to compare the histone acetylation modification and SRF enrichment in sodium butyrate (NaB) pretreatment group with control cells. Results The expression levels of α-SMA, Calponin and SM-MHC were increased in MSCs co-cultured with SMCs. H3K9ace, H3ace and H4ace in the promoter of these genes were enriched (P<0.05), and then, HDAC1 and HDAC2 in the induced MSCs were correspondingly decreased significantly (P<0.05). The enrichment of SRF in the NaB pretreatment group shared the same increasing tendency with H3K9ace and H4ace. Conclusion SRF may bind to H3K9ace and H4ace, through the regulation of HDAC1 and HDAC2 to control the expression of SMCs specific genes, and thus promote the differentiation from MSCs to SMCs. |
| abstractGer |
Objective To explore the role and mechanism of serum response factor (SRF) in differentiation of rat bone marrow mesenchymal stem cells (MSCs) into bladder smooth muscle cells (SMCs). Methods Rat MSCs and SMCs were isolated respectively from the bone marrow and bladder of 4-week-old female SD rats by adherent method, and then co-cultured together in a Transwell chamber. After the MSCs were harvested at different time points, real-time PCR and immunofluorescense assay were used to detect the expression of SMCs specific marker genes in co-cultured MSCs. Furthermore, the enrichment of histone acetylation modification, histone deacetylase (HDAC) and SRF in the promoter of these genes were tested by chromatin immunoprecipitation assay (ChIP) q-PCR in co-cultured MSCs. Finally, ChIP-qPCR was used to compare the histone acetylation modification and SRF enrichment in sodium butyrate (NaB) pretreatment group with control cells. Results The expression levels of α-SMA, Calponin and SM-MHC were increased in MSCs co-cultured with SMCs. H3K9ace, H3ace and H4ace in the promoter of these genes were enriched (P<0.05), and then, HDAC1 and HDAC2 in the induced MSCs were correspondingly decreased significantly (P<0.05). The enrichment of SRF in the NaB pretreatment group shared the same increasing tendency with H3K9ace and H4ace. Conclusion SRF may bind to H3K9ace and H4ace, through the regulation of HDAC1 and HDAC2 to control the expression of SMCs specific genes, and thus promote the differentiation from MSCs to SMCs. |
| abstract_unstemmed |
Objective To explore the role and mechanism of serum response factor (SRF) in differentiation of rat bone marrow mesenchymal stem cells (MSCs) into bladder smooth muscle cells (SMCs). Methods Rat MSCs and SMCs were isolated respectively from the bone marrow and bladder of 4-week-old female SD rats by adherent method, and then co-cultured together in a Transwell chamber. After the MSCs were harvested at different time points, real-time PCR and immunofluorescense assay were used to detect the expression of SMCs specific marker genes in co-cultured MSCs. Furthermore, the enrichment of histone acetylation modification, histone deacetylase (HDAC) and SRF in the promoter of these genes were tested by chromatin immunoprecipitation assay (ChIP) q-PCR in co-cultured MSCs. Finally, ChIP-qPCR was used to compare the histone acetylation modification and SRF enrichment in sodium butyrate (NaB) pretreatment group with control cells. Results The expression levels of α-SMA, Calponin and SM-MHC were increased in MSCs co-cultured with SMCs. H3K9ace, H3ace and H4ace in the promoter of these genes were enriched (P<0.05), and then, HDAC1 and HDAC2 in the induced MSCs were correspondingly decreased significantly (P<0.05). The enrichment of SRF in the NaB pretreatment group shared the same increasing tendency with H3K9ace and H4ace. Conclusion SRF may bind to H3K9ace and H4ace, through the regulation of HDAC1 and HDAC2 to control the expression of SMCs specific genes, and thus promote the differentiation from MSCs to SMCs. |
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Serum response factor promotes differentiation of rat bone marrow mesenchymal stem cells into smooth muscle cells via histone acetylation |
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https://doi.org/10.16016/j.1000-5404.202008213 https://doaj.org/article/89101c8892b847c8902c471dbd37b766 https://aammt.tmmu.edu.cn/Upload/rhtml/202008213.htm https://doaj.org/toc/1000-5404 |
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2024-07-03T20:08:10.798Z |
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