Efficacy of Intraoperative Vein Graft Storage Solutions in Preserving Endothelial Cell Integrity during Coronary Artery Bypass Surgery

(1) Introduction: Intraoperative preservation solutions for saphenous vein grafts may influence the endothelial structure and increase the risk of graft failure after coronary surgery. The aim of the study was to compare the efficacy of three solutions in maintaining the endothelial cell integrity o...
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Gespeichert in:

Autor*in:	Francesca Toto [verfasserIn] Tiziano Torre [verfasserIn] Lucia Turchetto [verfasserIn] Viviana Lo Cicero [verfasserIn] Sabrina Soncin [verfasserIn] Catherine Klersy [verfasserIn] Stefanos Demertzis [verfasserIn] Enrico Ferrari [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	coronary artery bypass grafting saphenous vein graft vein graft disease vein graft failure

Übergeordnetes Werk:	In: Journal of Clinical Medicine - MDPI AG, 2013, 11(2022), 4, p 1093
Übergeordnetes Werk:	volume:11 ; year:2022 ; number:4, p 1093

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/jcm11041093

Katalog-ID:	DOAJ013894471

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520			\|a (1) Introduction: Intraoperative preservation solutions for saphenous vein grafts may influence the endothelial structure and increase the risk of graft failure after coronary surgery. The aim of the study was to compare the efficacy of three solutions in maintaining the endothelial cell integrity of venous segments. (2) Methods: We tested the efficacy of physiological saline solution (PSS), heparinized autologous blood (HAB) and DuraGraft<sup<®</sup< in preserving the endothelium of vein segments by evaluating the degree of endothelial cell apoptosis. Two incubation times (2 and 4 h from harvesting) were used for each solution. The quantification of apoptotic cells was computed as the intensity nuclei/intensity area ratio. (3) Results: After 2 h of ischemia, the degree of apoptosis decreased progressively across the use of DuraGraft, HAB and PSS (<i<p</i< = 0.004), although only the difference between DuraGraft and PSS yielded a statistical significance (<i<p</i< = 0.002). After 4 h, a similar decrease in apoptosis was shown across the three media; however, statistical significance was not reached. The analysis of the elapsed time (2 or 4 h of incubation) showed that this was a relevant factor in maintaining the endothelial structural integrity independently from the storage solution (test for interaction of media and time <i<p</i< = 0.010). (4) Conclusion: Within 2 h of incubation, endothelial structural integrity depended on the incubating medium. DuraGraft better protected the SVG against ischemic-induced apoptosis when compared to saline solution. Prolonged ischemia was associated with extended endothelium damage and none of the studied solutions protected the vein graft.
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allfields	10.3390/jcm11041093 doi (DE-627)DOAJ013894471 (DE-599)DOAJba75a5cb34ab4e10840542ef3182e594 DE-627 ger DE-627 rakwb eng Francesca Toto verfasserin aut Efficacy of Intraoperative Vein Graft Storage Solutions in Preserving Endothelial Cell Integrity during Coronary Artery Bypass Surgery 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier (1) Introduction: Intraoperative preservation solutions for saphenous vein grafts may influence the endothelial structure and increase the risk of graft failure after coronary surgery. The aim of the study was to compare the efficacy of three solutions in maintaining the endothelial cell integrity of venous segments. (2) Methods: We tested the efficacy of physiological saline solution (PSS), heparinized autologous blood (HAB) and DuraGraft<sup<®</sup< in preserving the endothelium of vein segments by evaluating the degree of endothelial cell apoptosis. Two incubation times (2 and 4 h from harvesting) were used for each solution. The quantification of apoptotic cells was computed as the intensity nuclei/intensity area ratio. (3) Results: After 2 h of ischemia, the degree of apoptosis decreased progressively across the use of DuraGraft, HAB and PSS (<i<p</i< = 0.004), although only the difference between DuraGraft and PSS yielded a statistical significance (<i<p</i< = 0.002). After 4 h, a similar decrease in apoptosis was shown across the three media; however, statistical significance was not reached. The analysis of the elapsed time (2 or 4 h of incubation) showed that this was a relevant factor in maintaining the endothelial structural integrity independently from the storage solution (test for interaction of media and time <i<p</i< = 0.010). (4) Conclusion: Within 2 h of incubation, endothelial structural integrity depended on the incubating medium. DuraGraft better protected the SVG against ischemic-induced apoptosis when compared to saline solution. Prolonged ischemia was associated with extended endothelium damage and none of the studied solutions protected the vein graft. coronary artery bypass grafting saphenous vein graft vein graft disease vein graft failure Medicine R Tiziano Torre verfasserin aut Lucia Turchetto verfasserin aut Viviana Lo Cicero verfasserin aut Sabrina Soncin verfasserin aut Catherine Klersy verfasserin aut Stefanos Demertzis verfasserin aut Enrico Ferrari verfasserin aut In Journal of Clinical Medicine MDPI AG, 2013 11(2022), 4, p 1093 (DE-627)718632478 (DE-600)2662592-1 20770383 nnns volume:11 year:2022 number:4, p 1093 https://doi.org/10.3390/jcm11041093 kostenfrei https://doaj.org/article/ba75a5cb34ab4e10840542ef3182e594 kostenfrei https://www.mdpi.com/2077-0383/11/4/1093 kostenfrei https://doaj.org/toc/2077-0383 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 4, p 1093
spelling	10.3390/jcm11041093 doi (DE-627)DOAJ013894471 (DE-599)DOAJba75a5cb34ab4e10840542ef3182e594 DE-627 ger DE-627 rakwb eng Francesca Toto verfasserin aut Efficacy of Intraoperative Vein Graft Storage Solutions in Preserving Endothelial Cell Integrity during Coronary Artery Bypass Surgery 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier (1) Introduction: Intraoperative preservation solutions for saphenous vein grafts may influence the endothelial structure and increase the risk of graft failure after coronary surgery. The aim of the study was to compare the efficacy of three solutions in maintaining the endothelial cell integrity of venous segments. (2) Methods: We tested the efficacy of physiological saline solution (PSS), heparinized autologous blood (HAB) and DuraGraft<sup<®</sup< in preserving the endothelium of vein segments by evaluating the degree of endothelial cell apoptosis. Two incubation times (2 and 4 h from harvesting) were used for each solution. The quantification of apoptotic cells was computed as the intensity nuclei/intensity area ratio. (3) Results: After 2 h of ischemia, the degree of apoptosis decreased progressively across the use of DuraGraft, HAB and PSS (<i<p</i< = 0.004), although only the difference between DuraGraft and PSS yielded a statistical significance (<i<p</i< = 0.002). After 4 h, a similar decrease in apoptosis was shown across the three media; however, statistical significance was not reached. The analysis of the elapsed time (2 or 4 h of incubation) showed that this was a relevant factor in maintaining the endothelial structural integrity independently from the storage solution (test for interaction of media and time <i<p</i< = 0.010). (4) Conclusion: Within 2 h of incubation, endothelial structural integrity depended on the incubating medium. DuraGraft better protected the SVG against ischemic-induced apoptosis when compared to saline solution. Prolonged ischemia was associated with extended endothelium damage and none of the studied solutions protected the vein graft. coronary artery bypass grafting saphenous vein graft vein graft disease vein graft failure Medicine R Tiziano Torre verfasserin aut Lucia Turchetto verfasserin aut Viviana Lo Cicero verfasserin aut Sabrina Soncin verfasserin aut Catherine Klersy verfasserin aut Stefanos Demertzis verfasserin aut Enrico Ferrari verfasserin aut In Journal of Clinical Medicine MDPI AG, 2013 11(2022), 4, p 1093 (DE-627)718632478 (DE-600)2662592-1 20770383 nnns volume:11 year:2022 number:4, p 1093 https://doi.org/10.3390/jcm11041093 kostenfrei https://doaj.org/article/ba75a5cb34ab4e10840542ef3182e594 kostenfrei https://www.mdpi.com/2077-0383/11/4/1093 kostenfrei https://doaj.org/toc/2077-0383 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 4, p 1093
allfields_unstemmed	10.3390/jcm11041093 doi (DE-627)DOAJ013894471 (DE-599)DOAJba75a5cb34ab4e10840542ef3182e594 DE-627 ger DE-627 rakwb eng Francesca Toto verfasserin aut Efficacy of Intraoperative Vein Graft Storage Solutions in Preserving Endothelial Cell Integrity during Coronary Artery Bypass Surgery 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier (1) Introduction: Intraoperative preservation solutions for saphenous vein grafts may influence the endothelial structure and increase the risk of graft failure after coronary surgery. The aim of the study was to compare the efficacy of three solutions in maintaining the endothelial cell integrity of venous segments. (2) Methods: We tested the efficacy of physiological saline solution (PSS), heparinized autologous blood (HAB) and DuraGraft<sup<®</sup< in preserving the endothelium of vein segments by evaluating the degree of endothelial cell apoptosis. Two incubation times (2 and 4 h from harvesting) were used for each solution. The quantification of apoptotic cells was computed as the intensity nuclei/intensity area ratio. (3) Results: After 2 h of ischemia, the degree of apoptosis decreased progressively across the use of DuraGraft, HAB and PSS (<i<p</i< = 0.004), although only the difference between DuraGraft and PSS yielded a statistical significance (<i<p</i< = 0.002). After 4 h, a similar decrease in apoptosis was shown across the three media; however, statistical significance was not reached. The analysis of the elapsed time (2 or 4 h of incubation) showed that this was a relevant factor in maintaining the endothelial structural integrity independently from the storage solution (test for interaction of media and time <i<p</i< = 0.010). (4) Conclusion: Within 2 h of incubation, endothelial structural integrity depended on the incubating medium. DuraGraft better protected the SVG against ischemic-induced apoptosis when compared to saline solution. Prolonged ischemia was associated with extended endothelium damage and none of the studied solutions protected the vein graft. coronary artery bypass grafting saphenous vein graft vein graft disease vein graft failure Medicine R Tiziano Torre verfasserin aut Lucia Turchetto verfasserin aut Viviana Lo Cicero verfasserin aut Sabrina Soncin verfasserin aut Catherine Klersy verfasserin aut Stefanos Demertzis verfasserin aut Enrico Ferrari verfasserin aut In Journal of Clinical Medicine MDPI AG, 2013 11(2022), 4, p 1093 (DE-627)718632478 (DE-600)2662592-1 20770383 nnns volume:11 year:2022 number:4, p 1093 https://doi.org/10.3390/jcm11041093 kostenfrei https://doaj.org/article/ba75a5cb34ab4e10840542ef3182e594 kostenfrei https://www.mdpi.com/2077-0383/11/4/1093 kostenfrei https://doaj.org/toc/2077-0383 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 4, p 1093
allfieldsGer	10.3390/jcm11041093 doi (DE-627)DOAJ013894471 (DE-599)DOAJba75a5cb34ab4e10840542ef3182e594 DE-627 ger DE-627 rakwb eng Francesca Toto verfasserin aut Efficacy of Intraoperative Vein Graft Storage Solutions in Preserving Endothelial Cell Integrity during Coronary Artery Bypass Surgery 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier (1) Introduction: Intraoperative preservation solutions for saphenous vein grafts may influence the endothelial structure and increase the risk of graft failure after coronary surgery. The aim of the study was to compare the efficacy of three solutions in maintaining the endothelial cell integrity of venous segments. (2) Methods: We tested the efficacy of physiological saline solution (PSS), heparinized autologous blood (HAB) and DuraGraft<sup<®</sup< in preserving the endothelium of vein segments by evaluating the degree of endothelial cell apoptosis. Two incubation times (2 and 4 h from harvesting) were used for each solution. The quantification of apoptotic cells was computed as the intensity nuclei/intensity area ratio. (3) Results: After 2 h of ischemia, the degree of apoptosis decreased progressively across the use of DuraGraft, HAB and PSS (<i<p</i< = 0.004), although only the difference between DuraGraft and PSS yielded a statistical significance (<i<p</i< = 0.002). After 4 h, a similar decrease in apoptosis was shown across the three media; however, statistical significance was not reached. The analysis of the elapsed time (2 or 4 h of incubation) showed that this was a relevant factor in maintaining the endothelial structural integrity independently from the storage solution (test for interaction of media and time <i<p</i< = 0.010). (4) Conclusion: Within 2 h of incubation, endothelial structural integrity depended on the incubating medium. DuraGraft better protected the SVG against ischemic-induced apoptosis when compared to saline solution. Prolonged ischemia was associated with extended endothelium damage and none of the studied solutions protected the vein graft. coronary artery bypass grafting saphenous vein graft vein graft disease vein graft failure Medicine R Tiziano Torre verfasserin aut Lucia Turchetto verfasserin aut Viviana Lo Cicero verfasserin aut Sabrina Soncin verfasserin aut Catherine Klersy verfasserin aut Stefanos Demertzis verfasserin aut Enrico Ferrari verfasserin aut In Journal of Clinical Medicine MDPI AG, 2013 11(2022), 4, p 1093 (DE-627)718632478 (DE-600)2662592-1 20770383 nnns volume:11 year:2022 number:4, p 1093 https://doi.org/10.3390/jcm11041093 kostenfrei https://doaj.org/article/ba75a5cb34ab4e10840542ef3182e594 kostenfrei https://www.mdpi.com/2077-0383/11/4/1093 kostenfrei https://doaj.org/toc/2077-0383 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 4, p 1093
allfieldsSound	10.3390/jcm11041093 doi (DE-627)DOAJ013894471 (DE-599)DOAJba75a5cb34ab4e10840542ef3182e594 DE-627 ger DE-627 rakwb eng Francesca Toto verfasserin aut Efficacy of Intraoperative Vein Graft Storage Solutions in Preserving Endothelial Cell Integrity during Coronary Artery Bypass Surgery 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier (1) Introduction: Intraoperative preservation solutions for saphenous vein grafts may influence the endothelial structure and increase the risk of graft failure after coronary surgery. The aim of the study was to compare the efficacy of three solutions in maintaining the endothelial cell integrity of venous segments. (2) Methods: We tested the efficacy of physiological saline solution (PSS), heparinized autologous blood (HAB) and DuraGraft<sup<®</sup< in preserving the endothelium of vein segments by evaluating the degree of endothelial cell apoptosis. Two incubation times (2 and 4 h from harvesting) were used for each solution. The quantification of apoptotic cells was computed as the intensity nuclei/intensity area ratio. (3) Results: After 2 h of ischemia, the degree of apoptosis decreased progressively across the use of DuraGraft, HAB and PSS (<i<p</i< = 0.004), although only the difference between DuraGraft and PSS yielded a statistical significance (<i<p</i< = 0.002). After 4 h, a similar decrease in apoptosis was shown across the three media; however, statistical significance was not reached. The analysis of the elapsed time (2 or 4 h of incubation) showed that this was a relevant factor in maintaining the endothelial structural integrity independently from the storage solution (test for interaction of media and time <i<p</i< = 0.010). (4) Conclusion: Within 2 h of incubation, endothelial structural integrity depended on the incubating medium. DuraGraft better protected the SVG against ischemic-induced apoptosis when compared to saline solution. Prolonged ischemia was associated with extended endothelium damage and none of the studied solutions protected the vein graft. coronary artery bypass grafting saphenous vein graft vein graft disease vein graft failure Medicine R Tiziano Torre verfasserin aut Lucia Turchetto verfasserin aut Viviana Lo Cicero verfasserin aut Sabrina Soncin verfasserin aut Catherine Klersy verfasserin aut Stefanos Demertzis verfasserin aut Enrico Ferrari verfasserin aut In Journal of Clinical Medicine MDPI AG, 2013 11(2022), 4, p 1093 (DE-627)718632478 (DE-600)2662592-1 20770383 nnns volume:11 year:2022 number:4, p 1093 https://doi.org/10.3390/jcm11041093 kostenfrei https://doaj.org/article/ba75a5cb34ab4e10840542ef3182e594 kostenfrei https://www.mdpi.com/2077-0383/11/4/1093 kostenfrei https://doaj.org/toc/2077-0383 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 4, p 1093
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author	Francesca Toto
spellingShingle	Francesca Toto misc coronary artery bypass grafting misc saphenous vein graft misc vein graft disease misc vein graft failure misc Medicine misc R Efficacy of Intraoperative Vein Graft Storage Solutions in Preserving Endothelial Cell Integrity during Coronary Artery Bypass Surgery
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topic_title	Efficacy of Intraoperative Vein Graft Storage Solutions in Preserving Endothelial Cell Integrity during Coronary Artery Bypass Surgery coronary artery bypass grafting saphenous vein graft vein graft disease vein graft failure
topic	misc coronary artery bypass grafting misc saphenous vein graft misc vein graft disease misc vein graft failure misc Medicine misc R
topic_unstemmed	misc coronary artery bypass grafting misc saphenous vein graft misc vein graft disease misc vein graft failure misc Medicine misc R
topic_browse	misc coronary artery bypass grafting misc saphenous vein graft misc vein graft disease misc vein graft failure misc Medicine misc R
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title	Efficacy of Intraoperative Vein Graft Storage Solutions in Preserving Endothelial Cell Integrity during Coronary Artery Bypass Surgery
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title_full	Efficacy of Intraoperative Vein Graft Storage Solutions in Preserving Endothelial Cell Integrity during Coronary Artery Bypass Surgery
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author_browse	Francesca Toto Tiziano Torre Lucia Turchetto Viviana Lo Cicero Sabrina Soncin Catherine Klersy Stefanos Demertzis Enrico Ferrari
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title_sort	efficacy of intraoperative vein graft storage solutions in preserving endothelial cell integrity during coronary artery bypass surgery
title_auth	Efficacy of Intraoperative Vein Graft Storage Solutions in Preserving Endothelial Cell Integrity during Coronary Artery Bypass Surgery
abstract	(1) Introduction: Intraoperative preservation solutions for saphenous vein grafts may influence the endothelial structure and increase the risk of graft failure after coronary surgery. The aim of the study was to compare the efficacy of three solutions in maintaining the endothelial cell integrity of venous segments. (2) Methods: We tested the efficacy of physiological saline solution (PSS), heparinized autologous blood (HAB) and DuraGraft<sup<®</sup< in preserving the endothelium of vein segments by evaluating the degree of endothelial cell apoptosis. Two incubation times (2 and 4 h from harvesting) were used for each solution. The quantification of apoptotic cells was computed as the intensity nuclei/intensity area ratio. (3) Results: After 2 h of ischemia, the degree of apoptosis decreased progressively across the use of DuraGraft, HAB and PSS (<i<p</i< = 0.004), although only the difference between DuraGraft and PSS yielded a statistical significance (<i<p</i< = 0.002). After 4 h, a similar decrease in apoptosis was shown across the three media; however, statistical significance was not reached. The analysis of the elapsed time (2 or 4 h of incubation) showed that this was a relevant factor in maintaining the endothelial structural integrity independently from the storage solution (test for interaction of media and time <i<p</i< = 0.010). (4) Conclusion: Within 2 h of incubation, endothelial structural integrity depended on the incubating medium. DuraGraft better protected the SVG against ischemic-induced apoptosis when compared to saline solution. Prolonged ischemia was associated with extended endothelium damage and none of the studied solutions protected the vein graft.
abstractGer	(1) Introduction: Intraoperative preservation solutions for saphenous vein grafts may influence the endothelial structure and increase the risk of graft failure after coronary surgery. The aim of the study was to compare the efficacy of three solutions in maintaining the endothelial cell integrity of venous segments. (2) Methods: We tested the efficacy of physiological saline solution (PSS), heparinized autologous blood (HAB) and DuraGraft<sup<®</sup< in preserving the endothelium of vein segments by evaluating the degree of endothelial cell apoptosis. Two incubation times (2 and 4 h from harvesting) were used for each solution. The quantification of apoptotic cells was computed as the intensity nuclei/intensity area ratio. (3) Results: After 2 h of ischemia, the degree of apoptosis decreased progressively across the use of DuraGraft, HAB and PSS (<i<p</i< = 0.004), although only the difference between DuraGraft and PSS yielded a statistical significance (<i<p</i< = 0.002). After 4 h, a similar decrease in apoptosis was shown across the three media; however, statistical significance was not reached. The analysis of the elapsed time (2 or 4 h of incubation) showed that this was a relevant factor in maintaining the endothelial structural integrity independently from the storage solution (test for interaction of media and time <i<p</i< = 0.010). (4) Conclusion: Within 2 h of incubation, endothelial structural integrity depended on the incubating medium. DuraGraft better protected the SVG against ischemic-induced apoptosis when compared to saline solution. Prolonged ischemia was associated with extended endothelium damage and none of the studied solutions protected the vein graft.
abstract_unstemmed	(1) Introduction: Intraoperative preservation solutions for saphenous vein grafts may influence the endothelial structure and increase the risk of graft failure after coronary surgery. The aim of the study was to compare the efficacy of three solutions in maintaining the endothelial cell integrity of venous segments. (2) Methods: We tested the efficacy of physiological saline solution (PSS), heparinized autologous blood (HAB) and DuraGraft<sup<®</sup< in preserving the endothelium of vein segments by evaluating the degree of endothelial cell apoptosis. Two incubation times (2 and 4 h from harvesting) were used for each solution. The quantification of apoptotic cells was computed as the intensity nuclei/intensity area ratio. (3) Results: After 2 h of ischemia, the degree of apoptosis decreased progressively across the use of DuraGraft, HAB and PSS (<i<p</i< = 0.004), although only the difference between DuraGraft and PSS yielded a statistical significance (<i<p</i< = 0.002). After 4 h, a similar decrease in apoptosis was shown across the three media; however, statistical significance was not reached. The analysis of the elapsed time (2 or 4 h of incubation) showed that this was a relevant factor in maintaining the endothelial structural integrity independently from the storage solution (test for interaction of media and time <i<p</i< = 0.010). (4) Conclusion: Within 2 h of incubation, endothelial structural integrity depended on the incubating medium. DuraGraft better protected the SVG against ischemic-induced apoptosis when compared to saline solution. Prolonged ischemia was associated with extended endothelium damage and none of the studied solutions protected the vein graft.
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title_short	Efficacy of Intraoperative Vein Graft Storage Solutions in Preserving Endothelial Cell Integrity during Coronary Artery Bypass Surgery
url	https://doi.org/10.3390/jcm11041093 https://doaj.org/article/ba75a5cb34ab4e10840542ef3182e594 https://www.mdpi.com/2077-0383/11/4/1093 https://doaj.org/toc/2077-0383
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author2	Tiziano Torre Lucia Turchetto Viviana Lo Cicero Sabrina Soncin Catherine Klersy Stefanos Demertzis Enrico Ferrari
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up_date	2024-07-03T20:08:58.979Z
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The analysis of the elapsed time (2 or 4 h of incubation) showed that this was a relevant factor in maintaining the endothelial structural integrity independently from the storage solution (test for interaction of media and time <i<p</i< = 0.010). (4) Conclusion: Within 2 h of incubation, endothelial structural integrity depended on the incubating medium. DuraGraft better protected the SVG against ischemic-induced apoptosis when compared to saline solution. 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Efficacy of Intraoperative Vein Graft Storage Solutions in Preserving Endothelial Cell Integrity during Coronary Artery Bypass Surgery

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