In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential

ABSTRACT Vernonia condensata Baker has been traditionally used in folk medicine for the treatment of several inflammatory and infectious processes. Overexploitation of this plant species has drastically reduced its population in its natural habitat (Cerrado). Therefore, tissue culture tools, such as...
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Gespeichert in:

Autor*in:	Fabíola Rebouças Rodrigues [verfasserIn] Weliton Antonio Bastos de Almeida [verfasserIn] Carlos Alberto da Silva Ledo [verfasserIn] Taliane Leila Soares [verfasserIn] Mônica Lanzoni Rossi [verfasserIn] José Raniere Ferreira de Santana [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; Spanisch ; Portugiesisch

Erschienen:	2020

Schlagwörter:	Alumã in vitro culture of calli anatomical analysis scanning electron microscopy growth regulators.

Übergeordnetes Werk:	In: Ciência e Agrotecnologia - Universidade Federal de Lavras, 2007, 44(2020)
Übergeordnetes Werk:	volume:44 ; year:2020

Links:	Link aufrufen Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1590/1413-7054202044026719

Katalog-ID:	DOAJ014063395

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callnumber-first	S - Agriculture
author	Fabíola Rebouças Rodrigues
spellingShingle	Fabíola Rebouças Rodrigues misc S1-972 misc Alumã misc in vitro culture of calli misc anatomical analysis misc scanning electron microscopy misc growth regulators. misc Agriculture (General) In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential
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topic_title	S1-972 In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential Alumã in vitro culture of calli anatomical analysis scanning electron microscopy growth regulators
topic	misc S1-972 misc Alumã misc in vitro culture of calli misc anatomical analysis misc scanning electron microscopy misc growth regulators. misc Agriculture (General)
topic_unstemmed	misc S1-972 misc Alumã misc in vitro culture of calli misc anatomical analysis misc scanning electron microscopy misc growth regulators. misc Agriculture (General)
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title	In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential
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title_full	In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential
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title_sort	in vitro callus induction and development of vernonia condensata baker with embryogenic potential
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title_auth	In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential
abstract	ABSTRACT Vernonia condensata Baker has been traditionally used in folk medicine for the treatment of several inflammatory and infectious processes. Overexploitation of this plant species has drastically reduced its population in its natural habitat (Cerrado). Therefore, tissue culture tools, such as somatic embryogenesis, can be used as an alternative method for rapid and large-scale plant regeneration. The objectives of this study were to induce callogenesis in Vernonia condensata from different types of explants and to evaluate the structural aspects of the development of pro-embryogenic masses of this species by means of histological analyses. The formation of calli was induced from leaf explants and internodal segments, which were inoculated in EME medium supplemented with 50 g L-1 sucrose, 0.5 g L-1 malt extract and 2.68 μM NAA, plus varying concentrations of BAP (0.00, 2.22, 4.44 or 8.88 μM). After 40 days, the following morphogenetic traits were evaluated: intensity of callus formation, intensity of oxidation, callus texture, and morphogenesis. The calli with embryogenic masses were analyzed by light and scanning electron microscopy. Both types of explants were responsive regarding callogenesis, with the BAP concentration of 4.44 μM promoting the formation of friable calli associated with a larger percentage of calli with embryogenic masses. Cells from leaf explants and internodal segments were able to dedifferentiate and change into embryonic structures.
abstractGer	ABSTRACT Vernonia condensata Baker has been traditionally used in folk medicine for the treatment of several inflammatory and infectious processes. Overexploitation of this plant species has drastically reduced its population in its natural habitat (Cerrado). Therefore, tissue culture tools, such as somatic embryogenesis, can be used as an alternative method for rapid and large-scale plant regeneration. The objectives of this study were to induce callogenesis in Vernonia condensata from different types of explants and to evaluate the structural aspects of the development of pro-embryogenic masses of this species by means of histological analyses. The formation of calli was induced from leaf explants and internodal segments, which were inoculated in EME medium supplemented with 50 g L-1 sucrose, 0.5 g L-1 malt extract and 2.68 μM NAA, plus varying concentrations of BAP (0.00, 2.22, 4.44 or 8.88 μM). After 40 days, the following morphogenetic traits were evaluated: intensity of callus formation, intensity of oxidation, callus texture, and morphogenesis. The calli with embryogenic masses were analyzed by light and scanning electron microscopy. Both types of explants were responsive regarding callogenesis, with the BAP concentration of 4.44 μM promoting the formation of friable calli associated with a larger percentage of calli with embryogenic masses. Cells from leaf explants and internodal segments were able to dedifferentiate and change into embryonic structures.
abstract_unstemmed	ABSTRACT Vernonia condensata Baker has been traditionally used in folk medicine for the treatment of several inflammatory and infectious processes. Overexploitation of this plant species has drastically reduced its population in its natural habitat (Cerrado). Therefore, tissue culture tools, such as somatic embryogenesis, can be used as an alternative method for rapid and large-scale plant regeneration. The objectives of this study were to induce callogenesis in Vernonia condensata from different types of explants and to evaluate the structural aspects of the development of pro-embryogenic masses of this species by means of histological analyses. The formation of calli was induced from leaf explants and internodal segments, which were inoculated in EME medium supplemented with 50 g L-1 sucrose, 0.5 g L-1 malt extract and 2.68 μM NAA, plus varying concentrations of BAP (0.00, 2.22, 4.44 or 8.88 μM). After 40 days, the following morphogenetic traits were evaluated: intensity of callus formation, intensity of oxidation, callus texture, and morphogenesis. The calli with embryogenic masses were analyzed by light and scanning electron microscopy. Both types of explants were responsive regarding callogenesis, with the BAP concentration of 4.44 μM promoting the formation of friable calli associated with a larger percentage of calli with embryogenic masses. Cells from leaf explants and internodal segments were able to dedifferentiate and change into embryonic structures.
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title_short	In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential
url	https://doi.org/10.1590/1413-7054202044026719 https://doaj.org/article/67ca5ab2de624fc48ac5a2c17b7e6359 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542020000100201&tlng=en http://www.scielo.br/pdf/cagro/v44/1981-1829-cagro-44-e026719.pdf https://doaj.org/toc/1981-1829
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author2	Weliton Antonio Bastos de Almeida Carlos Alberto da Silva Ledo Taliane Leila Soares Mônica Lanzoni Rossi José Raniere Ferreira de Santana
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up_date	2024-07-03T21:04:04.444Z
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In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential

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