1 kHz fixed-target serial crystallography using a multilayer monochromator and an integrating pixel detector

Reliable sample delivery and efficient use of limited beam time have remained bottlenecks for serial crystallography (SX). Using a high-intensity polychromatic X-ray beam in combination with a newly developed charge-integrating JUNGFRAU detector, we have applied the method of fixed-target SX to coll...
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Autor*in:	A. Tolstikova [verfasserIn] M. Levantino [verfasserIn] O. Yefanov [verfasserIn] V. Hennicke [verfasserIn] P. Fischer [verfasserIn] J. Meyer [verfasserIn] A. Mozzanica [verfasserIn] S. Redford [verfasserIn] E. Crosas [verfasserIn] N. L. Opara [verfasserIn] M. Barthelmess [verfasserIn] J. Lieske [verfasserIn] D. Oberthuer [verfasserIn] E. Wator [verfasserIn] I. Mohacsi [verfasserIn] M. Wulff [verfasserIn] B. Schmitt [verfasserIn] H. N. Chapman [verfasserIn] A. Meents [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	serial crystallography synchrotron radiation pink beams protein crystallography protein structure structure determination

Übergeordnetes Werk:	In: IUCrJ - International Union of Crystallography, 2014, 6(2019), 5, Seite 927-937
Übergeordnetes Werk:	volume:6 ; year:2019 ; number:5 ; pages:927-937

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1107/S205225251900914X

Katalog-ID:	DOAJ014086646

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spelling	10.1107/S205225251900914X doi (DE-627)DOAJ014086646 (DE-599)DOAJ1ef4c15acf884be2838de3bc2b8b486b DE-627 ger DE-627 rakwb eng QD901-999 A. Tolstikova verfasserin aut 1 kHz fixed-target serial crystallography using a multilayer monochromator and an integrating pixel detector 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Reliable sample delivery and efficient use of limited beam time have remained bottlenecks for serial crystallography (SX). Using a high-intensity polychromatic X-ray beam in combination with a newly developed charge-integrating JUNGFRAU detector, we have applied the method of fixed-target SX to collect data at a rate of 1 kHz at a synchrotron-radiation facility. According to our data analysis for the given experimental conditions, only about 3 000 diffraction patterns are required for a high-quality diffraction dataset. With indexing rates of up to 25%, recording of such a dataset takes less than 30 s. serial crystallography synchrotron radiation pink beams protein crystallography protein structure structure determination Crystallography M. Levantino verfasserin aut O. Yefanov verfasserin aut V. Hennicke verfasserin aut P. Fischer verfasserin aut J. Meyer verfasserin aut A. Mozzanica verfasserin aut S. Redford verfasserin aut E. Crosas verfasserin aut N. L. Opara verfasserin aut M. Barthelmess verfasserin aut J. Lieske verfasserin aut D. Oberthuer verfasserin aut E. Wator verfasserin aut I. Mohacsi verfasserin aut M. Wulff verfasserin aut B. Schmitt verfasserin aut H. N. Chapman verfasserin aut A. Meents verfasserin aut In IUCrJ International Union of Crystallography, 2014 6(2019), 5, Seite 927-937 (DE-627)777782758 (DE-600)2754953-7 20522525 nnns volume:6 year:2019 number:5 pages:927-937 https://doi.org/10.1107/S205225251900914X kostenfrei https://doaj.org/article/1ef4c15acf884be2838de3bc2b8b486b kostenfrei http://scripts.iucr.org/cgi-bin/paper?S205225251900914X kostenfrei https://doaj.org/toc/2052-2525 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2019 5 927-937
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allfieldsSound	10.1107/S205225251900914X doi (DE-627)DOAJ014086646 (DE-599)DOAJ1ef4c15acf884be2838de3bc2b8b486b DE-627 ger DE-627 rakwb eng QD901-999 A. Tolstikova verfasserin aut 1 kHz fixed-target serial crystallography using a multilayer monochromator and an integrating pixel detector 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Reliable sample delivery and efficient use of limited beam time have remained bottlenecks for serial crystallography (SX). Using a high-intensity polychromatic X-ray beam in combination with a newly developed charge-integrating JUNGFRAU detector, we have applied the method of fixed-target SX to collect data at a rate of 1 kHz at a synchrotron-radiation facility. According to our data analysis for the given experimental conditions, only about 3 000 diffraction patterns are required for a high-quality diffraction dataset. With indexing rates of up to 25%, recording of such a dataset takes less than 30 s. serial crystallography synchrotron radiation pink beams protein crystallography protein structure structure determination Crystallography M. Levantino verfasserin aut O. Yefanov verfasserin aut V. Hennicke verfasserin aut P. Fischer verfasserin aut J. Meyer verfasserin aut A. Mozzanica verfasserin aut S. Redford verfasserin aut E. Crosas verfasserin aut N. L. Opara verfasserin aut M. Barthelmess verfasserin aut J. Lieske verfasserin aut D. Oberthuer verfasserin aut E. Wator verfasserin aut I. Mohacsi verfasserin aut M. Wulff verfasserin aut B. Schmitt verfasserin aut H. N. Chapman verfasserin aut A. Meents verfasserin aut In IUCrJ International Union of Crystallography, 2014 6(2019), 5, Seite 927-937 (DE-627)777782758 (DE-600)2754953-7 20522525 nnns volume:6 year:2019 number:5 pages:927-937 https://doi.org/10.1107/S205225251900914X kostenfrei https://doaj.org/article/1ef4c15acf884be2838de3bc2b8b486b kostenfrei http://scripts.iucr.org/cgi-bin/paper?S205225251900914X kostenfrei https://doaj.org/toc/2052-2525 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2019 5 927-937
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sourceStr	In IUCrJ 6(2019), 5, Seite 927-937 volume:6 year:2019 number:5 pages:927-937
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topic_facet	serial crystallography synchrotron radiation pink beams protein crystallography protein structure structure determination Crystallography
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authorswithroles_txt_mv	A. Tolstikova @@aut@@ M. Levantino @@aut@@ O. Yefanov @@aut@@ V. Hennicke @@aut@@ P. Fischer @@aut@@ J. Meyer @@aut@@ A. Mozzanica @@aut@@ S. Redford @@aut@@ E. Crosas @@aut@@ N. L. Opara @@aut@@ M. Barthelmess @@aut@@ J. Lieske @@aut@@ D. Oberthuer @@aut@@ E. Wator @@aut@@ I. Mohacsi @@aut@@ M. Wulff @@aut@@ B. Schmitt @@aut@@ H. N. Chapman @@aut@@ A. Meents @@aut@@
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spellingShingle	A. Tolstikova misc QD901-999 misc serial crystallography misc synchrotron radiation misc pink beams misc protein crystallography misc protein structure misc structure determination misc Crystallography 1 kHz fixed-target serial crystallography using a multilayer monochromator and an integrating pixel detector
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topic_title	QD901-999 1 kHz fixed-target serial crystallography using a multilayer monochromator and an integrating pixel detector serial crystallography synchrotron radiation pink beams protein crystallography protein structure structure determination
topic	misc QD901-999 misc serial crystallography misc synchrotron radiation misc pink beams misc protein crystallography misc protein structure misc structure determination misc Crystallography
topic_unstemmed	misc QD901-999 misc serial crystallography misc synchrotron radiation misc pink beams misc protein crystallography misc protein structure misc structure determination misc Crystallography
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title	1 kHz fixed-target serial crystallography using a multilayer monochromator and an integrating pixel detector
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author_browse	A. Tolstikova M. Levantino O. Yefanov V. Hennicke P. Fischer J. Meyer A. Mozzanica S. Redford E. Crosas N. L. Opara M. Barthelmess J. Lieske D. Oberthuer E. Wator I. Mohacsi M. Wulff B. Schmitt H. N. Chapman A. Meents
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title_sort	1 khz fixed-target serial crystallography using a multilayer monochromator and an integrating pixel detector
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title_auth	1 kHz fixed-target serial crystallography using a multilayer monochromator and an integrating pixel detector
abstract	Reliable sample delivery and efficient use of limited beam time have remained bottlenecks for serial crystallography (SX). Using a high-intensity polychromatic X-ray beam in combination with a newly developed charge-integrating JUNGFRAU detector, we have applied the method of fixed-target SX to collect data at a rate of 1 kHz at a synchrotron-radiation facility. According to our data analysis for the given experimental conditions, only about 3 000 diffraction patterns are required for a high-quality diffraction dataset. With indexing rates of up to 25%, recording of such a dataset takes less than 30 s.
abstractGer	Reliable sample delivery and efficient use of limited beam time have remained bottlenecks for serial crystallography (SX). Using a high-intensity polychromatic X-ray beam in combination with a newly developed charge-integrating JUNGFRAU detector, we have applied the method of fixed-target SX to collect data at a rate of 1 kHz at a synchrotron-radiation facility. According to our data analysis for the given experimental conditions, only about 3 000 diffraction patterns are required for a high-quality diffraction dataset. With indexing rates of up to 25%, recording of such a dataset takes less than 30 s.
abstract_unstemmed	Reliable sample delivery and efficient use of limited beam time have remained bottlenecks for serial crystallography (SX). Using a high-intensity polychromatic X-ray beam in combination with a newly developed charge-integrating JUNGFRAU detector, we have applied the method of fixed-target SX to collect data at a rate of 1 kHz at a synchrotron-radiation facility. According to our data analysis for the given experimental conditions, only about 3 000 diffraction patterns are required for a high-quality diffraction dataset. With indexing rates of up to 25%, recording of such a dataset takes less than 30 s.
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title_short	1 kHz fixed-target serial crystallography using a multilayer monochromator and an integrating pixel detector
url	https://doi.org/10.1107/S205225251900914X https://doaj.org/article/1ef4c15acf884be2838de3bc2b8b486b http://scripts.iucr.org/cgi-bin/paper?S205225251900914X https://doaj.org/toc/2052-2525
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1 kHz fixed-target serial crystallography using a multilayer monochromator and an integrating pixel detector

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