Multipotent Stromal Cells from Subcutaneous Adipose Tissue of Normal Weight and Obese Subjects: Modulation of Their Adipogenic Differentiation by Adenosine A<sub<1</sub< Receptor Ligands

Adenosine A<sub<1</sub< receptor (A<sub<1</sub<R) activation, stimulating lipogenesis and decreasing insulin resistance, could be useful for metabolic syndrome management in obese subjects. Since full A<sub<1</sub<R agonists induce harmful side-effects, while part...
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Autor*in:	Mariachiara Zuccarini [verfasserIn] Catia Lambertucci [verfasserIn] Marzia Carluccio [verfasserIn] Patricia Giuliani [verfasserIn] Maurizio Ronci [verfasserIn] Andrea Spinaci [verfasserIn] Rosaria Volpini [verfasserIn] Renata Ciccarelli [verfasserIn] Patrizia Di Iorio [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	adipose stromal cells (ASCs) subcutaneous adipose tissue adenosine A adipogenic differentiation adipogenic markers

Übergeordnetes Werk:	In: Cells - MDPI AG, 2012, 10(2021), 12, p 3560
Übergeordnetes Werk:	volume:10 ; year:2021 ; number:12, p 3560

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/cells10123560

Katalog-ID:	DOAJ014198509

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allfields	10.3390/cells10123560 doi (DE-627)DOAJ014198509 (DE-599)DOAJeadce9d5aa25496ea2a74c68af0bc765 DE-627 ger DE-627 rakwb eng QH573-671 Mariachiara Zuccarini verfasserin aut Multipotent Stromal Cells from Subcutaneous Adipose Tissue of Normal Weight and Obese Subjects: Modulation of Their Adipogenic Differentiation by Adenosine A<sub<1</sub< Receptor Ligands 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Adenosine A<sub<1</sub< receptor (A<sub<1</sub<R) activation, stimulating lipogenesis and decreasing insulin resistance, could be useful for metabolic syndrome management in obese subjects. Since full A<sub<1</sub<R agonists induce harmful side-effects, while partial agonists show a better pharmacological profile, we investigated the influence of two derivatives of the full A<sub<1</sub<R agonist 2-chloro-<i<N</i<<sup<6</sup<-cyclopentyladenosine (CCPA), C1 and C2 behaving as A<sub<1</sub<R partial agonists in animal models, on the adipogenic differentiation of stromal/stem cells (ASCs) from human subcutaneous adipose tissue, which mainly contribute to increase fat mass in obesity. The ASCs from normal-weight subjects showed increased proliferation and A<sub<1</sub<R expression but reduced adipogenic differentiation compared to obese individual-derived ASCs. Cell exposure to CCPA, C1, C2 or DPCPX, an A<sub<1</sub<R antagonist, did not affect ASC proliferation, while mainly C2 and DPCPX significantly decreased adipogenic differentiation of both ASC types, reducing the activity of glycerol-3-phosphate dehydrogenase and the expression of PPARγ and FABP-4, all adipogenic markers, and phosphorylation of Akt in the phosphatidylinositol-3-kinase pathway, which plays a key-role in adipogenesis. While requiring confirmation in in vivo models, our results suggest that A<sub<1</sub<R partial agonists or antagonists, by limiting ASC differentiation into adipocytes and, thereby, fat mass expansion, could favor development/worsening of metabolic syndrome in obese subjects without a dietary control. adipose stromal cells (ASCs) subcutaneous adipose tissue adenosine A<sub<1</sub< receptors adipogenic differentiation adipogenic markers Cytology Catia Lambertucci verfasserin aut Marzia Carluccio verfasserin aut Patricia Giuliani verfasserin aut Maurizio Ronci verfasserin aut Andrea Spinaci verfasserin aut Rosaria Volpini verfasserin aut Renata Ciccarelli verfasserin aut Patrizia Di Iorio verfasserin aut In Cells MDPI AG, 2012 10(2021), 12, p 3560 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:12, p 3560 https://doi.org/10.3390/cells10123560 kostenfrei https://doaj.org/article/eadce9d5aa25496ea2a74c68af0bc765 kostenfrei https://www.mdpi.com/2073-4409/10/12/3560 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 12, p 3560
spelling	10.3390/cells10123560 doi (DE-627)DOAJ014198509 (DE-599)DOAJeadce9d5aa25496ea2a74c68af0bc765 DE-627 ger DE-627 rakwb eng QH573-671 Mariachiara Zuccarini verfasserin aut Multipotent Stromal Cells from Subcutaneous Adipose Tissue of Normal Weight and Obese Subjects: Modulation of Their Adipogenic Differentiation by Adenosine A<sub<1</sub< Receptor Ligands 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Adenosine A<sub<1</sub< receptor (A<sub<1</sub<R) activation, stimulating lipogenesis and decreasing insulin resistance, could be useful for metabolic syndrome management in obese subjects. Since full A<sub<1</sub<R agonists induce harmful side-effects, while partial agonists show a better pharmacological profile, we investigated the influence of two derivatives of the full A<sub<1</sub<R agonist 2-chloro-<i<N</i<<sup<6</sup<-cyclopentyladenosine (CCPA), C1 and C2 behaving as A<sub<1</sub<R partial agonists in animal models, on the adipogenic differentiation of stromal/stem cells (ASCs) from human subcutaneous adipose tissue, which mainly contribute to increase fat mass in obesity. The ASCs from normal-weight subjects showed increased proliferation and A<sub<1</sub<R expression but reduced adipogenic differentiation compared to obese individual-derived ASCs. Cell exposure to CCPA, C1, C2 or DPCPX, an A<sub<1</sub<R antagonist, did not affect ASC proliferation, while mainly C2 and DPCPX significantly decreased adipogenic differentiation of both ASC types, reducing the activity of glycerol-3-phosphate dehydrogenase and the expression of PPARγ and FABP-4, all adipogenic markers, and phosphorylation of Akt in the phosphatidylinositol-3-kinase pathway, which plays a key-role in adipogenesis. While requiring confirmation in in vivo models, our results suggest that A<sub<1</sub<R partial agonists or antagonists, by limiting ASC differentiation into adipocytes and, thereby, fat mass expansion, could favor development/worsening of metabolic syndrome in obese subjects without a dietary control. adipose stromal cells (ASCs) subcutaneous adipose tissue adenosine A<sub<1</sub< receptors adipogenic differentiation adipogenic markers Cytology Catia Lambertucci verfasserin aut Marzia Carluccio verfasserin aut Patricia Giuliani verfasserin aut Maurizio Ronci verfasserin aut Andrea Spinaci verfasserin aut Rosaria Volpini verfasserin aut Renata Ciccarelli verfasserin aut Patrizia Di Iorio verfasserin aut In Cells MDPI AG, 2012 10(2021), 12, p 3560 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:12, p 3560 https://doi.org/10.3390/cells10123560 kostenfrei https://doaj.org/article/eadce9d5aa25496ea2a74c68af0bc765 kostenfrei https://www.mdpi.com/2073-4409/10/12/3560 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 12, p 3560
allfields_unstemmed	10.3390/cells10123560 doi (DE-627)DOAJ014198509 (DE-599)DOAJeadce9d5aa25496ea2a74c68af0bc765 DE-627 ger DE-627 rakwb eng QH573-671 Mariachiara Zuccarini verfasserin aut Multipotent Stromal Cells from Subcutaneous Adipose Tissue of Normal Weight and Obese Subjects: Modulation of Their Adipogenic Differentiation by Adenosine A<sub<1</sub< Receptor Ligands 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Adenosine A<sub<1</sub< receptor (A<sub<1</sub<R) activation, stimulating lipogenesis and decreasing insulin resistance, could be useful for metabolic syndrome management in obese subjects. Since full A<sub<1</sub<R agonists induce harmful side-effects, while partial agonists show a better pharmacological profile, we investigated the influence of two derivatives of the full A<sub<1</sub<R agonist 2-chloro-<i<N</i<<sup<6</sup<-cyclopentyladenosine (CCPA), C1 and C2 behaving as A<sub<1</sub<R partial agonists in animal models, on the adipogenic differentiation of stromal/stem cells (ASCs) from human subcutaneous adipose tissue, which mainly contribute to increase fat mass in obesity. The ASCs from normal-weight subjects showed increased proliferation and A<sub<1</sub<R expression but reduced adipogenic differentiation compared to obese individual-derived ASCs. Cell exposure to CCPA, C1, C2 or DPCPX, an A<sub<1</sub<R antagonist, did not affect ASC proliferation, while mainly C2 and DPCPX significantly decreased adipogenic differentiation of both ASC types, reducing the activity of glycerol-3-phosphate dehydrogenase and the expression of PPARγ and FABP-4, all adipogenic markers, and phosphorylation of Akt in the phosphatidylinositol-3-kinase pathway, which plays a key-role in adipogenesis. While requiring confirmation in in vivo models, our results suggest that A<sub<1</sub<R partial agonists or antagonists, by limiting ASC differentiation into adipocytes and, thereby, fat mass expansion, could favor development/worsening of metabolic syndrome in obese subjects without a dietary control. adipose stromal cells (ASCs) subcutaneous adipose tissue adenosine A<sub<1</sub< receptors adipogenic differentiation adipogenic markers Cytology Catia Lambertucci verfasserin aut Marzia Carluccio verfasserin aut Patricia Giuliani verfasserin aut Maurizio Ronci verfasserin aut Andrea Spinaci verfasserin aut Rosaria Volpini verfasserin aut Renata Ciccarelli verfasserin aut Patrizia Di Iorio verfasserin aut In Cells MDPI AG, 2012 10(2021), 12, p 3560 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:12, p 3560 https://doi.org/10.3390/cells10123560 kostenfrei https://doaj.org/article/eadce9d5aa25496ea2a74c68af0bc765 kostenfrei https://www.mdpi.com/2073-4409/10/12/3560 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 12, p 3560
allfieldsGer	10.3390/cells10123560 doi (DE-627)DOAJ014198509 (DE-599)DOAJeadce9d5aa25496ea2a74c68af0bc765 DE-627 ger DE-627 rakwb eng QH573-671 Mariachiara Zuccarini verfasserin aut Multipotent Stromal Cells from Subcutaneous Adipose Tissue of Normal Weight and Obese Subjects: Modulation of Their Adipogenic Differentiation by Adenosine A<sub<1</sub< Receptor Ligands 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Adenosine A<sub<1</sub< receptor (A<sub<1</sub<R) activation, stimulating lipogenesis and decreasing insulin resistance, could be useful for metabolic syndrome management in obese subjects. Since full A<sub<1</sub<R agonists induce harmful side-effects, while partial agonists show a better pharmacological profile, we investigated the influence of two derivatives of the full A<sub<1</sub<R agonist 2-chloro-<i<N</i<<sup<6</sup<-cyclopentyladenosine (CCPA), C1 and C2 behaving as A<sub<1</sub<R partial agonists in animal models, on the adipogenic differentiation of stromal/stem cells (ASCs) from human subcutaneous adipose tissue, which mainly contribute to increase fat mass in obesity. The ASCs from normal-weight subjects showed increased proliferation and A<sub<1</sub<R expression but reduced adipogenic differentiation compared to obese individual-derived ASCs. Cell exposure to CCPA, C1, C2 or DPCPX, an A<sub<1</sub<R antagonist, did not affect ASC proliferation, while mainly C2 and DPCPX significantly decreased adipogenic differentiation of both ASC types, reducing the activity of glycerol-3-phosphate dehydrogenase and the expression of PPARγ and FABP-4, all adipogenic markers, and phosphorylation of Akt in the phosphatidylinositol-3-kinase pathway, which plays a key-role in adipogenesis. While requiring confirmation in in vivo models, our results suggest that A<sub<1</sub<R partial agonists or antagonists, by limiting ASC differentiation into adipocytes and, thereby, fat mass expansion, could favor development/worsening of metabolic syndrome in obese subjects without a dietary control. adipose stromal cells (ASCs) subcutaneous adipose tissue adenosine A<sub<1</sub< receptors adipogenic differentiation adipogenic markers Cytology Catia Lambertucci verfasserin aut Marzia Carluccio verfasserin aut Patricia Giuliani verfasserin aut Maurizio Ronci verfasserin aut Andrea Spinaci verfasserin aut Rosaria Volpini verfasserin aut Renata Ciccarelli verfasserin aut Patrizia Di Iorio verfasserin aut In Cells MDPI AG, 2012 10(2021), 12, p 3560 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:12, p 3560 https://doi.org/10.3390/cells10123560 kostenfrei https://doaj.org/article/eadce9d5aa25496ea2a74c68af0bc765 kostenfrei https://www.mdpi.com/2073-4409/10/12/3560 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 12, p 3560
allfieldsSound	10.3390/cells10123560 doi (DE-627)DOAJ014198509 (DE-599)DOAJeadce9d5aa25496ea2a74c68af0bc765 DE-627 ger DE-627 rakwb eng QH573-671 Mariachiara Zuccarini verfasserin aut Multipotent Stromal Cells from Subcutaneous Adipose Tissue of Normal Weight and Obese Subjects: Modulation of Their Adipogenic Differentiation by Adenosine A<sub<1</sub< Receptor Ligands 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Adenosine A<sub<1</sub< receptor (A<sub<1</sub<R) activation, stimulating lipogenesis and decreasing insulin resistance, could be useful for metabolic syndrome management in obese subjects. Since full A<sub<1</sub<R agonists induce harmful side-effects, while partial agonists show a better pharmacological profile, we investigated the influence of two derivatives of the full A<sub<1</sub<R agonist 2-chloro-<i<N</i<<sup<6</sup<-cyclopentyladenosine (CCPA), C1 and C2 behaving as A<sub<1</sub<R partial agonists in animal models, on the adipogenic differentiation of stromal/stem cells (ASCs) from human subcutaneous adipose tissue, which mainly contribute to increase fat mass in obesity. The ASCs from normal-weight subjects showed increased proliferation and A<sub<1</sub<R expression but reduced adipogenic differentiation compared to obese individual-derived ASCs. Cell exposure to CCPA, C1, C2 or DPCPX, an A<sub<1</sub<R antagonist, did not affect ASC proliferation, while mainly C2 and DPCPX significantly decreased adipogenic differentiation of both ASC types, reducing the activity of glycerol-3-phosphate dehydrogenase and the expression of PPARγ and FABP-4, all adipogenic markers, and phosphorylation of Akt in the phosphatidylinositol-3-kinase pathway, which plays a key-role in adipogenesis. While requiring confirmation in in vivo models, our results suggest that A<sub<1</sub<R partial agonists or antagonists, by limiting ASC differentiation into adipocytes and, thereby, fat mass expansion, could favor development/worsening of metabolic syndrome in obese subjects without a dietary control. adipose stromal cells (ASCs) subcutaneous adipose tissue adenosine A<sub<1</sub< receptors adipogenic differentiation adipogenic markers Cytology Catia Lambertucci verfasserin aut Marzia Carluccio verfasserin aut Patricia Giuliani verfasserin aut Maurizio Ronci verfasserin aut Andrea Spinaci verfasserin aut Rosaria Volpini verfasserin aut Renata Ciccarelli verfasserin aut Patrizia Di Iorio verfasserin aut In Cells MDPI AG, 2012 10(2021), 12, p 3560 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:12, p 3560 https://doi.org/10.3390/cells10123560 kostenfrei https://doaj.org/article/eadce9d5aa25496ea2a74c68af0bc765 kostenfrei https://www.mdpi.com/2073-4409/10/12/3560 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 12, p 3560
language	English
source	In Cells 10(2021), 12, p 3560 volume:10 year:2021 number:12, p 3560
sourceStr	In Cells 10(2021), 12, p 3560 volume:10 year:2021 number:12, p 3560
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	adipose stromal cells (ASCs) subcutaneous adipose tissue adenosine A<sub<1</sub< receptors adipogenic differentiation adipogenic markers Cytology
isfreeaccess_bool	true
container_title	Cells
authorswithroles_txt_mv	Mariachiara Zuccarini @@aut@@ Catia Lambertucci @@aut@@ Marzia Carluccio @@aut@@ Patricia Giuliani @@aut@@ Maurizio Ronci @@aut@@ Andrea Spinaci @@aut@@ Rosaria Volpini @@aut@@ Renata Ciccarelli @@aut@@ Patrizia Di Iorio @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
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topic_title	QH573-671 Multipotent Stromal Cells from Subcutaneous Adipose Tissue of Normal Weight and Obese Subjects: Modulation of Their Adipogenic Differentiation by Adenosine A<sub<1</sub< Receptor Ligands adipose stromal cells (ASCs) subcutaneous adipose tissue adenosine A<sub<1</sub< receptors adipogenic differentiation adipogenic markers
topic	misc QH573-671 misc adipose stromal cells (ASCs) misc subcutaneous adipose tissue misc adenosine A<sub<1</sub< receptors misc adipogenic differentiation misc adipogenic markers misc Cytology
topic_unstemmed	misc QH573-671 misc adipose stromal cells (ASCs) misc subcutaneous adipose tissue misc adenosine A<sub<1</sub< receptors misc adipogenic differentiation misc adipogenic markers misc Cytology
topic_browse	misc QH573-671 misc adipose stromal cells (ASCs) misc subcutaneous adipose tissue misc adenosine A<sub<1</sub< receptors misc adipogenic differentiation misc adipogenic markers misc Cytology
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title	Multipotent Stromal Cells from Subcutaneous Adipose Tissue of Normal Weight and Obese Subjects: Modulation of Their Adipogenic Differentiation by Adenosine A<sub<1</sub< Receptor Ligands
ctrlnum	(DE-627)DOAJ014198509 (DE-599)DOAJeadce9d5aa25496ea2a74c68af0bc765
title_full	Multipotent Stromal Cells from Subcutaneous Adipose Tissue of Normal Weight and Obese Subjects: Modulation of Their Adipogenic Differentiation by Adenosine A<sub<1</sub< Receptor Ligands
author_sort	Mariachiara Zuccarini
journal	Cells
journalStr	Cells
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author_browse	Mariachiara Zuccarini Catia Lambertucci Marzia Carluccio Patricia Giuliani Maurizio Ronci Andrea Spinaci Rosaria Volpini Renata Ciccarelli Patrizia Di Iorio
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format_se	Elektronische Aufsätze
author-letter	Mariachiara Zuccarini
doi_str_mv	10.3390/cells10123560
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title_sort	multipotent stromal cells from subcutaneous adipose tissue of normal weight and obese subjects: modulation of their adipogenic differentiation by adenosine a<sub<1</sub< receptor ligands
callnumber	QH573-671
title_auth	Multipotent Stromal Cells from Subcutaneous Adipose Tissue of Normal Weight and Obese Subjects: Modulation of Their Adipogenic Differentiation by Adenosine A<sub<1</sub< Receptor Ligands
abstract	Adenosine A<sub<1</sub< receptor (A<sub<1</sub<R) activation, stimulating lipogenesis and decreasing insulin resistance, could be useful for metabolic syndrome management in obese subjects. Since full A<sub<1</sub<R agonists induce harmful side-effects, while partial agonists show a better pharmacological profile, we investigated the influence of two derivatives of the full A<sub<1</sub<R agonist 2-chloro-<i<N</i<<sup<6</sup<-cyclopentyladenosine (CCPA), C1 and C2 behaving as A<sub<1</sub<R partial agonists in animal models, on the adipogenic differentiation of stromal/stem cells (ASCs) from human subcutaneous adipose tissue, which mainly contribute to increase fat mass in obesity. The ASCs from normal-weight subjects showed increased proliferation and A<sub<1</sub<R expression but reduced adipogenic differentiation compared to obese individual-derived ASCs. Cell exposure to CCPA, C1, C2 or DPCPX, an A<sub<1</sub<R antagonist, did not affect ASC proliferation, while mainly C2 and DPCPX significantly decreased adipogenic differentiation of both ASC types, reducing the activity of glycerol-3-phosphate dehydrogenase and the expression of PPARγ and FABP-4, all adipogenic markers, and phosphorylation of Akt in the phosphatidylinositol-3-kinase pathway, which plays a key-role in adipogenesis. While requiring confirmation in in vivo models, our results suggest that A<sub<1</sub<R partial agonists or antagonists, by limiting ASC differentiation into adipocytes and, thereby, fat mass expansion, could favor development/worsening of metabolic syndrome in obese subjects without a dietary control.
abstractGer	Adenosine A<sub<1</sub< receptor (A<sub<1</sub<R) activation, stimulating lipogenesis and decreasing insulin resistance, could be useful for metabolic syndrome management in obese subjects. Since full A<sub<1</sub<R agonists induce harmful side-effects, while partial agonists show a better pharmacological profile, we investigated the influence of two derivatives of the full A<sub<1</sub<R agonist 2-chloro-<i<N</i<<sup<6</sup<-cyclopentyladenosine (CCPA), C1 and C2 behaving as A<sub<1</sub<R partial agonists in animal models, on the adipogenic differentiation of stromal/stem cells (ASCs) from human subcutaneous adipose tissue, which mainly contribute to increase fat mass in obesity. The ASCs from normal-weight subjects showed increased proliferation and A<sub<1</sub<R expression but reduced adipogenic differentiation compared to obese individual-derived ASCs. Cell exposure to CCPA, C1, C2 or DPCPX, an A<sub<1</sub<R antagonist, did not affect ASC proliferation, while mainly C2 and DPCPX significantly decreased adipogenic differentiation of both ASC types, reducing the activity of glycerol-3-phosphate dehydrogenase and the expression of PPARγ and FABP-4, all adipogenic markers, and phosphorylation of Akt in the phosphatidylinositol-3-kinase pathway, which plays a key-role in adipogenesis. While requiring confirmation in in vivo models, our results suggest that A<sub<1</sub<R partial agonists or antagonists, by limiting ASC differentiation into adipocytes and, thereby, fat mass expansion, could favor development/worsening of metabolic syndrome in obese subjects without a dietary control.
abstract_unstemmed	Adenosine A<sub<1</sub< receptor (A<sub<1</sub<R) activation, stimulating lipogenesis and decreasing insulin resistance, could be useful for metabolic syndrome management in obese subjects. Since full A<sub<1</sub<R agonists induce harmful side-effects, while partial agonists show a better pharmacological profile, we investigated the influence of two derivatives of the full A<sub<1</sub<R agonist 2-chloro-<i<N</i<<sup<6</sup<-cyclopentyladenosine (CCPA), C1 and C2 behaving as A<sub<1</sub<R partial agonists in animal models, on the adipogenic differentiation of stromal/stem cells (ASCs) from human subcutaneous adipose tissue, which mainly contribute to increase fat mass in obesity. The ASCs from normal-weight subjects showed increased proliferation and A<sub<1</sub<R expression but reduced adipogenic differentiation compared to obese individual-derived ASCs. Cell exposure to CCPA, C1, C2 or DPCPX, an A<sub<1</sub<R antagonist, did not affect ASC proliferation, while mainly C2 and DPCPX significantly decreased adipogenic differentiation of both ASC types, reducing the activity of glycerol-3-phosphate dehydrogenase and the expression of PPARγ and FABP-4, all adipogenic markers, and phosphorylation of Akt in the phosphatidylinositol-3-kinase pathway, which plays a key-role in adipogenesis. While requiring confirmation in in vivo models, our results suggest that A<sub<1</sub<R partial agonists or antagonists, by limiting ASC differentiation into adipocytes and, thereby, fat mass expansion, could favor development/worsening of metabolic syndrome in obese subjects without a dietary control.
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title_short	Multipotent Stromal Cells from Subcutaneous Adipose Tissue of Normal Weight and Obese Subjects: Modulation of Their Adipogenic Differentiation by Adenosine A<sub<1</sub< Receptor Ligands
url	https://doi.org/10.3390/cells10123560 https://doaj.org/article/eadce9d5aa25496ea2a74c68af0bc765 https://www.mdpi.com/2073-4409/10/12/3560 https://doaj.org/toc/2073-4409
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author2	Catia Lambertucci Marzia Carluccio Patricia Giuliani Maurizio Ronci Andrea Spinaci Rosaria Volpini Renata Ciccarelli Patrizia Di Iorio
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up_date	2024-07-03T21:47:51.034Z
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Multipotent Stromal Cells from Subcutaneous Adipose Tissue of Normal Weight and Obese Subjects: Modulation of Their Adipogenic Differentiation by Adenosine A<sub<1</sub< Receptor Ligands

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