The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays
Intravital microscopy and other direct-imaging techniques have allowed for a characterisation of leukocyte migration that has revolutionised the field of immunology, resulting in an unprecedented understanding of the mechanisms of immune response and adaptive immunity. However, there is an assumptio...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Jennifer Frattolin [verfasserIn] Daniel J. Watson [verfasserIn] Willy V. Bonneuil [verfasserIn] Matthew J. Russell [verfasserIn] Francesca Fasanella Masci [verfasserIn] Mikaila Bandara [verfasserIn] Bindi S. Brook [verfasserIn] Robert J. B. Nibbs [verfasserIn] James E. Moore [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2021 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Cells - MDPI AG, 2012, 10(2021), 12, p 3439 |
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| Übergeordnetes Werk: |
volume:10 ; year:2021 ; number:12, p 3439 |
| Links: |
|---|
| DOI / URN: |
10.3390/cells10123439 |
|---|
| Katalog-ID: |
DOAJ01420133X |
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| 520 | |a Intravital microscopy and other direct-imaging techniques have allowed for a characterisation of leukocyte migration that has revolutionised the field of immunology, resulting in an unprecedented understanding of the mechanisms of immune response and adaptive immunity. However, there is an assumption within the field that modern imaging techniques permit imaging parameters where the resulting cell track accurately captures a cell’s motion. This notion is almost entirely untested, and the relationship between what could be observed at a given scale and the underlying cell behaviour is undefined. Insufficient spatial and temporal resolutions within migration assays can result in misrepresentation of important physiologic processes or cause subtle changes in critical cell behaviour to be missed. In this review, we contextualise how scale can affect the perceived migratory behaviour of cells, summarise the limited approaches to mitigate this effect, and establish the need for a widely implemented framework to account for scale and correct observations of cell motion. We then extend the concept of scale to new approaches that seek to bridge the current “black box” between single-cell behaviour and systemic response. | ||
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10.3390/cells10123439 doi (DE-627)DOAJ01420133X (DE-599)DOAJ63448ba70e68496cb2a64fb9af9f17c3 DE-627 ger DE-627 rakwb eng QH573-671 Jennifer Frattolin verfasserin aut The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Intravital microscopy and other direct-imaging techniques have allowed for a characterisation of leukocyte migration that has revolutionised the field of immunology, resulting in an unprecedented understanding of the mechanisms of immune response and adaptive immunity. However, there is an assumption within the field that modern imaging techniques permit imaging parameters where the resulting cell track accurately captures a cell’s motion. This notion is almost entirely untested, and the relationship between what could be observed at a given scale and the underlying cell behaviour is undefined. Insufficient spatial and temporal resolutions within migration assays can result in misrepresentation of important physiologic processes or cause subtle changes in critical cell behaviour to be missed. In this review, we contextualise how scale can affect the perceived migratory behaviour of cells, summarise the limited approaches to mitigate this effect, and establish the need for a widely implemented framework to account for scale and correct observations of cell motion. We then extend the concept of scale to new approaches that seek to bridge the current “black box” between single-cell behaviour and systemic response. cell migration chemotaxis chemokine leukocytes scale live cell tracking Cytology Daniel J. Watson verfasserin aut Willy V. Bonneuil verfasserin aut Matthew J. Russell verfasserin aut Francesca Fasanella Masci verfasserin aut Mikaila Bandara verfasserin aut Bindi S. Brook verfasserin aut Robert J. B. Nibbs verfasserin aut James E. Moore verfasserin aut In Cells MDPI AG, 2012 10(2021), 12, p 3439 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:12, p 3439 https://doi.org/10.3390/cells10123439 kostenfrei https://doaj.org/article/63448ba70e68496cb2a64fb9af9f17c3 kostenfrei https://www.mdpi.com/2073-4409/10/12/3439 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 12, p 3439 |
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10.3390/cells10123439 doi (DE-627)DOAJ01420133X (DE-599)DOAJ63448ba70e68496cb2a64fb9af9f17c3 DE-627 ger DE-627 rakwb eng QH573-671 Jennifer Frattolin verfasserin aut The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Intravital microscopy and other direct-imaging techniques have allowed for a characterisation of leukocyte migration that has revolutionised the field of immunology, resulting in an unprecedented understanding of the mechanisms of immune response and adaptive immunity. However, there is an assumption within the field that modern imaging techniques permit imaging parameters where the resulting cell track accurately captures a cell’s motion. This notion is almost entirely untested, and the relationship between what could be observed at a given scale and the underlying cell behaviour is undefined. Insufficient spatial and temporal resolutions within migration assays can result in misrepresentation of important physiologic processes or cause subtle changes in critical cell behaviour to be missed. In this review, we contextualise how scale can affect the perceived migratory behaviour of cells, summarise the limited approaches to mitigate this effect, and establish the need for a widely implemented framework to account for scale and correct observations of cell motion. We then extend the concept of scale to new approaches that seek to bridge the current “black box” between single-cell behaviour and systemic response. cell migration chemotaxis chemokine leukocytes scale live cell tracking Cytology Daniel J. Watson verfasserin aut Willy V. Bonneuil verfasserin aut Matthew J. Russell verfasserin aut Francesca Fasanella Masci verfasserin aut Mikaila Bandara verfasserin aut Bindi S. Brook verfasserin aut Robert J. B. Nibbs verfasserin aut James E. Moore verfasserin aut In Cells MDPI AG, 2012 10(2021), 12, p 3439 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:12, p 3439 https://doi.org/10.3390/cells10123439 kostenfrei https://doaj.org/article/63448ba70e68496cb2a64fb9af9f17c3 kostenfrei https://www.mdpi.com/2073-4409/10/12/3439 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 12, p 3439 |
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10.3390/cells10123439 doi (DE-627)DOAJ01420133X (DE-599)DOAJ63448ba70e68496cb2a64fb9af9f17c3 DE-627 ger DE-627 rakwb eng QH573-671 Jennifer Frattolin verfasserin aut The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Intravital microscopy and other direct-imaging techniques have allowed for a characterisation of leukocyte migration that has revolutionised the field of immunology, resulting in an unprecedented understanding of the mechanisms of immune response and adaptive immunity. However, there is an assumption within the field that modern imaging techniques permit imaging parameters where the resulting cell track accurately captures a cell’s motion. This notion is almost entirely untested, and the relationship between what could be observed at a given scale and the underlying cell behaviour is undefined. Insufficient spatial and temporal resolutions within migration assays can result in misrepresentation of important physiologic processes or cause subtle changes in critical cell behaviour to be missed. In this review, we contextualise how scale can affect the perceived migratory behaviour of cells, summarise the limited approaches to mitigate this effect, and establish the need for a widely implemented framework to account for scale and correct observations of cell motion. We then extend the concept of scale to new approaches that seek to bridge the current “black box” between single-cell behaviour and systemic response. cell migration chemotaxis chemokine leukocytes scale live cell tracking Cytology Daniel J. Watson verfasserin aut Willy V. Bonneuil verfasserin aut Matthew J. Russell verfasserin aut Francesca Fasanella Masci verfasserin aut Mikaila Bandara verfasserin aut Bindi S. Brook verfasserin aut Robert J. B. Nibbs verfasserin aut James E. Moore verfasserin aut In Cells MDPI AG, 2012 10(2021), 12, p 3439 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:12, p 3439 https://doi.org/10.3390/cells10123439 kostenfrei https://doaj.org/article/63448ba70e68496cb2a64fb9af9f17c3 kostenfrei https://www.mdpi.com/2073-4409/10/12/3439 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 12, p 3439 |
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10.3390/cells10123439 doi (DE-627)DOAJ01420133X (DE-599)DOAJ63448ba70e68496cb2a64fb9af9f17c3 DE-627 ger DE-627 rakwb eng QH573-671 Jennifer Frattolin verfasserin aut The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Intravital microscopy and other direct-imaging techniques have allowed for a characterisation of leukocyte migration that has revolutionised the field of immunology, resulting in an unprecedented understanding of the mechanisms of immune response and adaptive immunity. However, there is an assumption within the field that modern imaging techniques permit imaging parameters where the resulting cell track accurately captures a cell’s motion. This notion is almost entirely untested, and the relationship between what could be observed at a given scale and the underlying cell behaviour is undefined. Insufficient spatial and temporal resolutions within migration assays can result in misrepresentation of important physiologic processes or cause subtle changes in critical cell behaviour to be missed. In this review, we contextualise how scale can affect the perceived migratory behaviour of cells, summarise the limited approaches to mitigate this effect, and establish the need for a widely implemented framework to account for scale and correct observations of cell motion. We then extend the concept of scale to new approaches that seek to bridge the current “black box” between single-cell behaviour and systemic response. cell migration chemotaxis chemokine leukocytes scale live cell tracking Cytology Daniel J. Watson verfasserin aut Willy V. Bonneuil verfasserin aut Matthew J. Russell verfasserin aut Francesca Fasanella Masci verfasserin aut Mikaila Bandara verfasserin aut Bindi S. Brook verfasserin aut Robert J. B. Nibbs verfasserin aut James E. Moore verfasserin aut In Cells MDPI AG, 2012 10(2021), 12, p 3439 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:12, p 3439 https://doi.org/10.3390/cells10123439 kostenfrei https://doaj.org/article/63448ba70e68496cb2a64fb9af9f17c3 kostenfrei https://www.mdpi.com/2073-4409/10/12/3439 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 12, p 3439 |
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10.3390/cells10123439 doi (DE-627)DOAJ01420133X (DE-599)DOAJ63448ba70e68496cb2a64fb9af9f17c3 DE-627 ger DE-627 rakwb eng QH573-671 Jennifer Frattolin verfasserin aut The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Intravital microscopy and other direct-imaging techniques have allowed for a characterisation of leukocyte migration that has revolutionised the field of immunology, resulting in an unprecedented understanding of the mechanisms of immune response and adaptive immunity. However, there is an assumption within the field that modern imaging techniques permit imaging parameters where the resulting cell track accurately captures a cell’s motion. This notion is almost entirely untested, and the relationship between what could be observed at a given scale and the underlying cell behaviour is undefined. Insufficient spatial and temporal resolutions within migration assays can result in misrepresentation of important physiologic processes or cause subtle changes in critical cell behaviour to be missed. In this review, we contextualise how scale can affect the perceived migratory behaviour of cells, summarise the limited approaches to mitigate this effect, and establish the need for a widely implemented framework to account for scale and correct observations of cell motion. We then extend the concept of scale to new approaches that seek to bridge the current “black box” between single-cell behaviour and systemic response. cell migration chemotaxis chemokine leukocytes scale live cell tracking Cytology Daniel J. Watson verfasserin aut Willy V. Bonneuil verfasserin aut Matthew J. Russell verfasserin aut Francesca Fasanella Masci verfasserin aut Mikaila Bandara verfasserin aut Bindi S. Brook verfasserin aut Robert J. B. Nibbs verfasserin aut James E. Moore verfasserin aut In Cells MDPI AG, 2012 10(2021), 12, p 3439 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:12, p 3439 https://doi.org/10.3390/cells10123439 kostenfrei https://doaj.org/article/63448ba70e68496cb2a64fb9af9f17c3 kostenfrei https://www.mdpi.com/2073-4409/10/12/3439 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 12, p 3439 |
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Intravital microscopy and other direct-imaging techniques have allowed for a characterisation of leukocyte migration that has revolutionised the field of immunology, resulting in an unprecedented understanding of the mechanisms of immune response and adaptive immunity. However, there is an assumption within the field that modern imaging techniques permit imaging parameters where the resulting cell track accurately captures a cell’s motion. This notion is almost entirely untested, and the relationship between what could be observed at a given scale and the underlying cell behaviour is undefined. Insufficient spatial and temporal resolutions within migration assays can result in misrepresentation of important physiologic processes or cause subtle changes in critical cell behaviour to be missed. In this review, we contextualise how scale can affect the perceived migratory behaviour of cells, summarise the limited approaches to mitigate this effect, and establish the need for a widely implemented framework to account for scale and correct observations of cell motion. We then extend the concept of scale to new approaches that seek to bridge the current “black box” between single-cell behaviour and systemic response. |
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Intravital microscopy and other direct-imaging techniques have allowed for a characterisation of leukocyte migration that has revolutionised the field of immunology, resulting in an unprecedented understanding of the mechanisms of immune response and adaptive immunity. However, there is an assumption within the field that modern imaging techniques permit imaging parameters where the resulting cell track accurately captures a cell’s motion. This notion is almost entirely untested, and the relationship between what could be observed at a given scale and the underlying cell behaviour is undefined. Insufficient spatial and temporal resolutions within migration assays can result in misrepresentation of important physiologic processes or cause subtle changes in critical cell behaviour to be missed. In this review, we contextualise how scale can affect the perceived migratory behaviour of cells, summarise the limited approaches to mitigate this effect, and establish the need for a widely implemented framework to account for scale and correct observations of cell motion. We then extend the concept of scale to new approaches that seek to bridge the current “black box” between single-cell behaviour and systemic response. |
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Intravital microscopy and other direct-imaging techniques have allowed for a characterisation of leukocyte migration that has revolutionised the field of immunology, resulting in an unprecedented understanding of the mechanisms of immune response and adaptive immunity. However, there is an assumption within the field that modern imaging techniques permit imaging parameters where the resulting cell track accurately captures a cell’s motion. This notion is almost entirely untested, and the relationship between what could be observed at a given scale and the underlying cell behaviour is undefined. Insufficient spatial and temporal resolutions within migration assays can result in misrepresentation of important physiologic processes or cause subtle changes in critical cell behaviour to be missed. In this review, we contextualise how scale can affect the perceived migratory behaviour of cells, summarise the limited approaches to mitigate this effect, and establish the need for a widely implemented framework to account for scale and correct observations of cell motion. We then extend the concept of scale to new approaches that seek to bridge the current “black box” between single-cell behaviour and systemic response. |
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