Free-ranging pigs identified as a multi-reservoir of Trypanosoma brucei and Trypanosoma congolense in the Vavoua area, a historical sleeping sickness focus of Côte d'Ivoire.

<h4<Background</h4<The existence of an animal reservoir of Trypanosoma brucei gambiense (T. b. gambiense), the agent of human African trypanosomiasis (HAT), may compromise the interruption of transmission targeted by World Health Organization. The aim of this study was to investigate the presence of trypanosomes in pigs and people in the Vavoua HAT historical focus where cases were still diagnosed in the early 2010's.<h4<Methods</h4<For the human survey, we used the CATT, mini-anion exchange centrifugation technique and immune trypanolysis tests. For the animal survey, the buffy coat technique was also used as well as the PCR using Trypanosoma species specific, including the T. b. gambiense TgsGP detection using single round and nested PCRs, performed from animal blood samples and from strains isolated from subjects positive for parasitological investigations.<h4<Results</h4<No HAT cases were detected among 345 people tested. A total of 167 pigs were investigated. Free-ranging pigs appeared significantly more infected than pigs in pen. Over 70% of free-ranging pigs were positive for CATT and parasitological investigations and 27-43% were positive to trypanolysis depending on the antigen used. T. brucei was the most prevalent species (57%) followed by T. congolense (24%). Blood sample extracted DNA of T. brucei positive subjects were negative to single round TgsGP PCR. However, 1/22 and 6/22 isolated strains were positive with single round and nested TgsGP PCRs, respectively.<h4<Discussion</h4<Free-ranging pigs were identified as a multi-reservoir of T. brucei and/or T. congolense with mixed infections of different strains. This trypanosome diversity hinders the easy and direct detection of T. b. gambiense. We highlight the lack of tools to prove or exclude with certainty the presence of T. b. gambiense. This study once more highlights the need of technical improvements to explore the role of animals in the epidemiology of HAT. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Barkissa Mélika Traoré [verfasserIn] Mathurin Koffi [verfasserIn] Martial Kassi N'Djetchi [verfasserIn] Dramane Kaba [verfasserIn] Jacques Kaboré [verfasserIn] Hamidou Ilboudo [verfasserIn] Bernadin Ahouty Ahouty [verfasserIn] Minayégninrin Koné [verfasserIn] Bamoro Coulibaly [verfasserIn] Thomas Konan [verfasserIn] Adeline Segard [verfasserIn] Lingué Kouakou [verfasserIn] Thierry De Meeûs [verfasserIn] Sophie Ravel [verfasserIn] Philippe Solano [verfasserIn] Jean-Mathieu Bart [verfasserIn] Vincent Jamonneau [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Übergeordnetes Werk:	In: PLoS Neglected Tropical Diseases - Public Library of Science (PLoS), 2008, 15(2021), 12, p e0010036
Übergeordnetes Werk:	volume:15 ; year:2021 ; number:12, p e0010036

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.1371/journal.pntd.0010036

Katalog-ID:	DOAJ014336081

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245	1	0	\|a Free-ranging pigs identified as a multi-reservoir of Trypanosoma brucei and Trypanosoma congolense in the Vavoua area, a historical sleeping sickness focus of Côte d'Ivoire.
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allfields	10.1371/journal.pntd.0010036 doi (DE-627)DOAJ014336081 (DE-599)DOAJ73d9f15683d444ec86ccc2de38b236ca DE-627 ger DE-627 rakwb eng RC955-962 RA1-1270 Barkissa Mélika Traoré verfasserin aut Free-ranging pigs identified as a multi-reservoir of Trypanosoma brucei and Trypanosoma congolense in the Vavoua area, a historical sleeping sickness focus of Côte d'Ivoire. 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <h4<Background</h4<The existence of an animal reservoir of Trypanosoma brucei gambiense (T. b. gambiense), the agent of human African trypanosomiasis (HAT), may compromise the interruption of transmission targeted by World Health Organization. The aim of this study was to investigate the presence of trypanosomes in pigs and people in the Vavoua HAT historical focus where cases were still diagnosed in the early 2010's.<h4<Methods</h4<For the human survey, we used the CATT, mini-anion exchange centrifugation technique and immune trypanolysis tests. For the animal survey, the buffy coat technique was also used as well as the PCR using Trypanosoma species specific, including the T. b. gambiense TgsGP detection using single round and nested PCRs, performed from animal blood samples and from strains isolated from subjects positive for parasitological investigations.<h4<Results</h4<No HAT cases were detected among 345 people tested. A total of 167 pigs were investigated. Free-ranging pigs appeared significantly more infected than pigs in pen. Over 70% of free-ranging pigs were positive for CATT and parasitological investigations and 27-43% were positive to trypanolysis depending on the antigen used. T. brucei was the most prevalent species (57%) followed by T. congolense (24%). Blood sample extracted DNA of T. brucei positive subjects were negative to single round TgsGP PCR. However, 1/22 and 6/22 isolated strains were positive with single round and nested TgsGP PCRs, respectively.<h4<Discussion</h4<Free-ranging pigs were identified as a multi-reservoir of T. brucei and/or T. congolense with mixed infections of different strains. This trypanosome diversity hinders the easy and direct detection of T. b. gambiense. We highlight the lack of tools to prove or exclude with certainty the presence of T. b. gambiense. This study once more highlights the need of technical improvements to explore the role of animals in the epidemiology of HAT. Arctic medicine. Tropical medicine Public aspects of medicine Mathurin Koffi verfasserin aut Martial Kassi N'Djetchi verfasserin aut Dramane Kaba verfasserin aut Jacques Kaboré verfasserin aut Hamidou Ilboudo verfasserin aut Bernadin Ahouty Ahouty verfasserin aut Minayégninrin Koné verfasserin aut Bamoro Coulibaly verfasserin aut Thomas Konan verfasserin aut Adeline Segard verfasserin aut Lingué Kouakou verfasserin aut Thierry De Meeûs verfasserin aut Sophie Ravel verfasserin aut Philippe Solano verfasserin aut Jean-Mathieu Bart verfasserin aut Vincent Jamonneau verfasserin aut In PLoS Neglected Tropical Diseases Public Library of Science (PLoS), 2008 15(2021), 12, p e0010036 (DE-627)568915356 (DE-600)2429704-5 19352735 nnns volume:15 year:2021 number:12, p e0010036 https://doi.org/10.1371/journal.pntd.0010036 kostenfrei https://doaj.org/article/73d9f15683d444ec86ccc2de38b236ca kostenfrei https://doi.org/10.1371/journal.pntd.0010036 kostenfrei https://doaj.org/toc/1935-2727 Journal toc kostenfrei https://doaj.org/toc/1935-2735 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2021 12, p e0010036
spelling	10.1371/journal.pntd.0010036 doi (DE-627)DOAJ014336081 (DE-599)DOAJ73d9f15683d444ec86ccc2de38b236ca DE-627 ger DE-627 rakwb eng RC955-962 RA1-1270 Barkissa Mélika Traoré verfasserin aut Free-ranging pigs identified as a multi-reservoir of Trypanosoma brucei and Trypanosoma congolense in the Vavoua area, a historical sleeping sickness focus of Côte d'Ivoire. 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <h4<Background</h4<The existence of an animal reservoir of Trypanosoma brucei gambiense (T. b. gambiense), the agent of human African trypanosomiasis (HAT), may compromise the interruption of transmission targeted by World Health Organization. The aim of this study was to investigate the presence of trypanosomes in pigs and people in the Vavoua HAT historical focus where cases were still diagnosed in the early 2010's.<h4<Methods</h4<For the human survey, we used the CATT, mini-anion exchange centrifugation technique and immune trypanolysis tests. For the animal survey, the buffy coat technique was also used as well as the PCR using Trypanosoma species specific, including the T. b. gambiense TgsGP detection using single round and nested PCRs, performed from animal blood samples and from strains isolated from subjects positive for parasitological investigations.<h4<Results</h4<No HAT cases were detected among 345 people tested. A total of 167 pigs were investigated. Free-ranging pigs appeared significantly more infected than pigs in pen. Over 70% of free-ranging pigs were positive for CATT and parasitological investigations and 27-43% were positive to trypanolysis depending on the antigen used. T. brucei was the most prevalent species (57%) followed by T. congolense (24%). Blood sample extracted DNA of T. brucei positive subjects were negative to single round TgsGP PCR. However, 1/22 and 6/22 isolated strains were positive with single round and nested TgsGP PCRs, respectively.<h4<Discussion</h4<Free-ranging pigs were identified as a multi-reservoir of T. brucei and/or T. congolense with mixed infections of different strains. This trypanosome diversity hinders the easy and direct detection of T. b. gambiense. We highlight the lack of tools to prove or exclude with certainty the presence of T. b. gambiense. This study once more highlights the need of technical improvements to explore the role of animals in the epidemiology of HAT. Arctic medicine. Tropical medicine Public aspects of medicine Mathurin Koffi verfasserin aut Martial Kassi N'Djetchi verfasserin aut Dramane Kaba verfasserin aut Jacques Kaboré verfasserin aut Hamidou Ilboudo verfasserin aut Bernadin Ahouty Ahouty verfasserin aut Minayégninrin Koné verfasserin aut Bamoro Coulibaly verfasserin aut Thomas Konan verfasserin aut Adeline Segard verfasserin aut Lingué Kouakou verfasserin aut Thierry De Meeûs verfasserin aut Sophie Ravel verfasserin aut Philippe Solano verfasserin aut Jean-Mathieu Bart verfasserin aut Vincent Jamonneau verfasserin aut In PLoS Neglected Tropical Diseases Public Library of Science (PLoS), 2008 15(2021), 12, p e0010036 (DE-627)568915356 (DE-600)2429704-5 19352735 nnns volume:15 year:2021 number:12, p e0010036 https://doi.org/10.1371/journal.pntd.0010036 kostenfrei https://doaj.org/article/73d9f15683d444ec86ccc2de38b236ca kostenfrei https://doi.org/10.1371/journal.pntd.0010036 kostenfrei https://doaj.org/toc/1935-2727 Journal toc kostenfrei https://doaj.org/toc/1935-2735 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2021 12, p e0010036
allfields_unstemmed	10.1371/journal.pntd.0010036 doi (DE-627)DOAJ014336081 (DE-599)DOAJ73d9f15683d444ec86ccc2de38b236ca DE-627 ger DE-627 rakwb eng RC955-962 RA1-1270 Barkissa Mélika Traoré verfasserin aut Free-ranging pigs identified as a multi-reservoir of Trypanosoma brucei and Trypanosoma congolense in the Vavoua area, a historical sleeping sickness focus of Côte d'Ivoire. 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <h4<Background</h4<The existence of an animal reservoir of Trypanosoma brucei gambiense (T. b. gambiense), the agent of human African trypanosomiasis (HAT), may compromise the interruption of transmission targeted by World Health Organization. The aim of this study was to investigate the presence of trypanosomes in pigs and people in the Vavoua HAT historical focus where cases were still diagnosed in the early 2010's.<h4<Methods</h4<For the human survey, we used the CATT, mini-anion exchange centrifugation technique and immune trypanolysis tests. For the animal survey, the buffy coat technique was also used as well as the PCR using Trypanosoma species specific, including the T. b. gambiense TgsGP detection using single round and nested PCRs, performed from animal blood samples and from strains isolated from subjects positive for parasitological investigations.<h4<Results</h4<No HAT cases were detected among 345 people tested. A total of 167 pigs were investigated. Free-ranging pigs appeared significantly more infected than pigs in pen. Over 70% of free-ranging pigs were positive for CATT and parasitological investigations and 27-43% were positive to trypanolysis depending on the antigen used. T. brucei was the most prevalent species (57%) followed by T. congolense (24%). Blood sample extracted DNA of T. brucei positive subjects were negative to single round TgsGP PCR. However, 1/22 and 6/22 isolated strains were positive with single round and nested TgsGP PCRs, respectively.<h4<Discussion</h4<Free-ranging pigs were identified as a multi-reservoir of T. brucei and/or T. congolense with mixed infections of different strains. This trypanosome diversity hinders the easy and direct detection of T. b. gambiense. We highlight the lack of tools to prove or exclude with certainty the presence of T. b. gambiense. This study once more highlights the need of technical improvements to explore the role of animals in the epidemiology of HAT. Arctic medicine. Tropical medicine Public aspects of medicine Mathurin Koffi verfasserin aut Martial Kassi N'Djetchi verfasserin aut Dramane Kaba verfasserin aut Jacques Kaboré verfasserin aut Hamidou Ilboudo verfasserin aut Bernadin Ahouty Ahouty verfasserin aut Minayégninrin Koné verfasserin aut Bamoro Coulibaly verfasserin aut Thomas Konan verfasserin aut Adeline Segard verfasserin aut Lingué Kouakou verfasserin aut Thierry De Meeûs verfasserin aut Sophie Ravel verfasserin aut Philippe Solano verfasserin aut Jean-Mathieu Bart verfasserin aut Vincent Jamonneau verfasserin aut In PLoS Neglected Tropical Diseases Public Library of Science (PLoS), 2008 15(2021), 12, p e0010036 (DE-627)568915356 (DE-600)2429704-5 19352735 nnns volume:15 year:2021 number:12, p e0010036 https://doi.org/10.1371/journal.pntd.0010036 kostenfrei https://doaj.org/article/73d9f15683d444ec86ccc2de38b236ca kostenfrei https://doi.org/10.1371/journal.pntd.0010036 kostenfrei https://doaj.org/toc/1935-2727 Journal toc kostenfrei https://doaj.org/toc/1935-2735 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2021 12, p e0010036
allfieldsGer	10.1371/journal.pntd.0010036 doi (DE-627)DOAJ014336081 (DE-599)DOAJ73d9f15683d444ec86ccc2de38b236ca DE-627 ger DE-627 rakwb eng RC955-962 RA1-1270 Barkissa Mélika Traoré verfasserin aut Free-ranging pigs identified as a multi-reservoir of Trypanosoma brucei and Trypanosoma congolense in the Vavoua area, a historical sleeping sickness focus of Côte d'Ivoire. 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <h4<Background</h4<The existence of an animal reservoir of Trypanosoma brucei gambiense (T. b. gambiense), the agent of human African trypanosomiasis (HAT), may compromise the interruption of transmission targeted by World Health Organization. The aim of this study was to investigate the presence of trypanosomes in pigs and people in the Vavoua HAT historical focus where cases were still diagnosed in the early 2010's.<h4<Methods</h4<For the human survey, we used the CATT, mini-anion exchange centrifugation technique and immune trypanolysis tests. For the animal survey, the buffy coat technique was also used as well as the PCR using Trypanosoma species specific, including the T. b. gambiense TgsGP detection using single round and nested PCRs, performed from animal blood samples and from strains isolated from subjects positive for parasitological investigations.<h4<Results</h4<No HAT cases were detected among 345 people tested. A total of 167 pigs were investigated. Free-ranging pigs appeared significantly more infected than pigs in pen. Over 70% of free-ranging pigs were positive for CATT and parasitological investigations and 27-43% were positive to trypanolysis depending on the antigen used. T. brucei was the most prevalent species (57%) followed by T. congolense (24%). Blood sample extracted DNA of T. brucei positive subjects were negative to single round TgsGP PCR. However, 1/22 and 6/22 isolated strains were positive with single round and nested TgsGP PCRs, respectively.<h4<Discussion</h4<Free-ranging pigs were identified as a multi-reservoir of T. brucei and/or T. congolense with mixed infections of different strains. This trypanosome diversity hinders the easy and direct detection of T. b. gambiense. We highlight the lack of tools to prove or exclude with certainty the presence of T. b. gambiense. This study once more highlights the need of technical improvements to explore the role of animals in the epidemiology of HAT. Arctic medicine. Tropical medicine Public aspects of medicine Mathurin Koffi verfasserin aut Martial Kassi N'Djetchi verfasserin aut Dramane Kaba verfasserin aut Jacques Kaboré verfasserin aut Hamidou Ilboudo verfasserin aut Bernadin Ahouty Ahouty verfasserin aut Minayégninrin Koné verfasserin aut Bamoro Coulibaly verfasserin aut Thomas Konan verfasserin aut Adeline Segard verfasserin aut Lingué Kouakou verfasserin aut Thierry De Meeûs verfasserin aut Sophie Ravel verfasserin aut Philippe Solano verfasserin aut Jean-Mathieu Bart verfasserin aut Vincent Jamonneau verfasserin aut In PLoS Neglected Tropical Diseases Public Library of Science (PLoS), 2008 15(2021), 12, p e0010036 (DE-627)568915356 (DE-600)2429704-5 19352735 nnns volume:15 year:2021 number:12, p e0010036 https://doi.org/10.1371/journal.pntd.0010036 kostenfrei https://doaj.org/article/73d9f15683d444ec86ccc2de38b236ca kostenfrei https://doi.org/10.1371/journal.pntd.0010036 kostenfrei https://doaj.org/toc/1935-2727 Journal toc kostenfrei https://doaj.org/toc/1935-2735 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2021 12, p e0010036
allfieldsSound	10.1371/journal.pntd.0010036 doi (DE-627)DOAJ014336081 (DE-599)DOAJ73d9f15683d444ec86ccc2de38b236ca DE-627 ger DE-627 rakwb eng RC955-962 RA1-1270 Barkissa Mélika Traoré verfasserin aut Free-ranging pigs identified as a multi-reservoir of Trypanosoma brucei and Trypanosoma congolense in the Vavoua area, a historical sleeping sickness focus of Côte d'Ivoire. 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <h4<Background</h4<The existence of an animal reservoir of Trypanosoma brucei gambiense (T. b. gambiense), the agent of human African trypanosomiasis (HAT), may compromise the interruption of transmission targeted by World Health Organization. The aim of this study was to investigate the presence of trypanosomes in pigs and people in the Vavoua HAT historical focus where cases were still diagnosed in the early 2010's.<h4<Methods</h4<For the human survey, we used the CATT, mini-anion exchange centrifugation technique and immune trypanolysis tests. For the animal survey, the buffy coat technique was also used as well as the PCR using Trypanosoma species specific, including the T. b. gambiense TgsGP detection using single round and nested PCRs, performed from animal blood samples and from strains isolated from subjects positive for parasitological investigations.<h4<Results</h4<No HAT cases were detected among 345 people tested. A total of 167 pigs were investigated. Free-ranging pigs appeared significantly more infected than pigs in pen. Over 70% of free-ranging pigs were positive for CATT and parasitological investigations and 27-43% were positive to trypanolysis depending on the antigen used. T. brucei was the most prevalent species (57%) followed by T. congolense (24%). Blood sample extracted DNA of T. brucei positive subjects were negative to single round TgsGP PCR. However, 1/22 and 6/22 isolated strains were positive with single round and nested TgsGP PCRs, respectively.<h4<Discussion</h4<Free-ranging pigs were identified as a multi-reservoir of T. brucei and/or T. congolense with mixed infections of different strains. This trypanosome diversity hinders the easy and direct detection of T. b. gambiense. We highlight the lack of tools to prove or exclude with certainty the presence of T. b. gambiense. This study once more highlights the need of technical improvements to explore the role of animals in the epidemiology of HAT. Arctic medicine. Tropical medicine Public aspects of medicine Mathurin Koffi verfasserin aut Martial Kassi N'Djetchi verfasserin aut Dramane Kaba verfasserin aut Jacques Kaboré verfasserin aut Hamidou Ilboudo verfasserin aut Bernadin Ahouty Ahouty verfasserin aut Minayégninrin Koné verfasserin aut Bamoro Coulibaly verfasserin aut Thomas Konan verfasserin aut Adeline Segard verfasserin aut Lingué Kouakou verfasserin aut Thierry De Meeûs verfasserin aut Sophie Ravel verfasserin aut Philippe Solano verfasserin aut Jean-Mathieu Bart verfasserin aut Vincent Jamonneau verfasserin aut In PLoS Neglected Tropical Diseases Public Library of Science (PLoS), 2008 15(2021), 12, p e0010036 (DE-627)568915356 (DE-600)2429704-5 19352735 nnns volume:15 year:2021 number:12, p e0010036 https://doi.org/10.1371/journal.pntd.0010036 kostenfrei https://doaj.org/article/73d9f15683d444ec86ccc2de38b236ca kostenfrei https://doi.org/10.1371/journal.pntd.0010036 kostenfrei https://doaj.org/toc/1935-2727 Journal toc kostenfrei https://doaj.org/toc/1935-2735 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2021 12, p e0010036
language	English
source	In PLoS Neglected Tropical Diseases 15(2021), 12, p e0010036 volume:15 year:2021 number:12, p e0010036
sourceStr	In PLoS Neglected Tropical Diseases 15(2021), 12, p e0010036 volume:15 year:2021 number:12, p e0010036
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Arctic medicine. Tropical medicine Public aspects of medicine
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container_title	PLoS Neglected Tropical Diseases
authorswithroles_txt_mv	Barkissa Mélika Traoré @@aut@@ Mathurin Koffi @@aut@@ Martial Kassi N'Djetchi @@aut@@ Dramane Kaba @@aut@@ Jacques Kaboré @@aut@@ Hamidou Ilboudo @@aut@@ Bernadin Ahouty Ahouty @@aut@@ Minayégninrin Koné @@aut@@ Bamoro Coulibaly @@aut@@ Thomas Konan @@aut@@ Adeline Segard @@aut@@ Lingué Kouakou @@aut@@ Thierry De Meeûs @@aut@@ Sophie Ravel @@aut@@ Philippe Solano @@aut@@ Jean-Mathieu Bart @@aut@@ Vincent Jamonneau @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
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The aim of this study was to investigate the presence of trypanosomes in pigs and people in the Vavoua HAT historical focus where cases were still diagnosed in the early 2010's.<h4<Methods</h4<For the human survey, we used the CATT, mini-anion exchange centrifugation technique and immune trypanolysis tests. For the animal survey, the buffy coat technique was also used as well as the PCR using Trypanosoma species specific, including the T. b. gambiense TgsGP detection using single round and nested PCRs, performed from animal blood samples and from strains isolated from subjects positive for parasitological investigations.<h4<Results</h4<No HAT cases were detected among 345 people tested. A total of 167 pigs were investigated. Free-ranging pigs appeared significantly more infected than pigs in pen. Over 70% of free-ranging pigs were positive for CATT and parasitological investigations and 27-43% were positive to trypanolysis depending on the antigen used. 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callnumber-first	R - Medicine
author	Barkissa Mélika Traoré
spellingShingle	Barkissa Mélika Traoré misc RC955-962 misc RA1-1270 misc Arctic medicine. Tropical medicine misc Public aspects of medicine Free-ranging pigs identified as a multi-reservoir of Trypanosoma brucei and Trypanosoma congolense in the Vavoua area, a historical sleeping sickness focus of Côte d'Ivoire.
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topic_title	RC955-962 RA1-1270 Free-ranging pigs identified as a multi-reservoir of Trypanosoma brucei and Trypanosoma congolense in the Vavoua area, a historical sleeping sickness focus of Côte d'Ivoire
topic	misc RC955-962 misc RA1-1270 misc Arctic medicine. Tropical medicine misc Public aspects of medicine
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title	Free-ranging pigs identified as a multi-reservoir of Trypanosoma brucei and Trypanosoma congolense in the Vavoua area, a historical sleeping sickness focus of Côte d'Ivoire.
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title_full	Free-ranging pigs identified as a multi-reservoir of Trypanosoma brucei and Trypanosoma congolense in the Vavoua area, a historical sleeping sickness focus of Côte d'Ivoire
author_sort	Barkissa Mélika Traoré
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author_browse	Barkissa Mélika Traoré Mathurin Koffi Martial Kassi N'Djetchi Dramane Kaba Jacques Kaboré Hamidou Ilboudo Bernadin Ahouty Ahouty Minayégninrin Koné Bamoro Coulibaly Thomas Konan Adeline Segard Lingué Kouakou Thierry De Meeûs Sophie Ravel Philippe Solano Jean-Mathieu Bart Vincent Jamonneau
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author-letter	Barkissa Mélika Traoré
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title_sort	free-ranging pigs identified as a multi-reservoir of trypanosoma brucei and trypanosoma congolense in the vavoua area, a historical sleeping sickness focus of côte d'ivoire
callnumber	RC955-962
title_auth	Free-ranging pigs identified as a multi-reservoir of Trypanosoma brucei and Trypanosoma congolense in the Vavoua area, a historical sleeping sickness focus of Côte d'Ivoire.
abstract	<h4<Background</h4<The existence of an animal reservoir of Trypanosoma brucei gambiense (T. b. gambiense), the agent of human African trypanosomiasis (HAT), may compromise the interruption of transmission targeted by World Health Organization. The aim of this study was to investigate the presence of trypanosomes in pigs and people in the Vavoua HAT historical focus where cases were still diagnosed in the early 2010's.<h4<Methods</h4<For the human survey, we used the CATT, mini-anion exchange centrifugation technique and immune trypanolysis tests. For the animal survey, the buffy coat technique was also used as well as the PCR using Trypanosoma species specific, including the T. b. gambiense TgsGP detection using single round and nested PCRs, performed from animal blood samples and from strains isolated from subjects positive for parasitological investigations.<h4<Results</h4<No HAT cases were detected among 345 people tested. A total of 167 pigs were investigated. Free-ranging pigs appeared significantly more infected than pigs in pen. Over 70% of free-ranging pigs were positive for CATT and parasitological investigations and 27-43% were positive to trypanolysis depending on the antigen used. T. brucei was the most prevalent species (57%) followed by T. congolense (24%). Blood sample extracted DNA of T. brucei positive subjects were negative to single round TgsGP PCR. However, 1/22 and 6/22 isolated strains were positive with single round and nested TgsGP PCRs, respectively.<h4<Discussion</h4<Free-ranging pigs were identified as a multi-reservoir of T. brucei and/or T. congolense with mixed infections of different strains. This trypanosome diversity hinders the easy and direct detection of T. b. gambiense. We highlight the lack of tools to prove or exclude with certainty the presence of T. b. gambiense. This study once more highlights the need of technical improvements to explore the role of animals in the epidemiology of HAT.
abstractGer	<h4<Background</h4<The existence of an animal reservoir of Trypanosoma brucei gambiense (T. b. gambiense), the agent of human African trypanosomiasis (HAT), may compromise the interruption of transmission targeted by World Health Organization. The aim of this study was to investigate the presence of trypanosomes in pigs and people in the Vavoua HAT historical focus where cases were still diagnosed in the early 2010's.<h4<Methods</h4<For the human survey, we used the CATT, mini-anion exchange centrifugation technique and immune trypanolysis tests. For the animal survey, the buffy coat technique was also used as well as the PCR using Trypanosoma species specific, including the T. b. gambiense TgsGP detection using single round and nested PCRs, performed from animal blood samples and from strains isolated from subjects positive for parasitological investigations.<h4<Results</h4<No HAT cases were detected among 345 people tested. A total of 167 pigs were investigated. Free-ranging pigs appeared significantly more infected than pigs in pen. Over 70% of free-ranging pigs were positive for CATT and parasitological investigations and 27-43% were positive to trypanolysis depending on the antigen used. T. brucei was the most prevalent species (57%) followed by T. congolense (24%). Blood sample extracted DNA of T. brucei positive subjects were negative to single round TgsGP PCR. However, 1/22 and 6/22 isolated strains were positive with single round and nested TgsGP PCRs, respectively.<h4<Discussion</h4<Free-ranging pigs were identified as a multi-reservoir of T. brucei and/or T. congolense with mixed infections of different strains. This trypanosome diversity hinders the easy and direct detection of T. b. gambiense. We highlight the lack of tools to prove or exclude with certainty the presence of T. b. gambiense. This study once more highlights the need of technical improvements to explore the role of animals in the epidemiology of HAT.
abstract_unstemmed	<h4<Background</h4<The existence of an animal reservoir of Trypanosoma brucei gambiense (T. b. gambiense), the agent of human African trypanosomiasis (HAT), may compromise the interruption of transmission targeted by World Health Organization. The aim of this study was to investigate the presence of trypanosomes in pigs and people in the Vavoua HAT historical focus where cases were still diagnosed in the early 2010's.<h4<Methods</h4<For the human survey, we used the CATT, mini-anion exchange centrifugation technique and immune trypanolysis tests. For the animal survey, the buffy coat technique was also used as well as the PCR using Trypanosoma species specific, including the T. b. gambiense TgsGP detection using single round and nested PCRs, performed from animal blood samples and from strains isolated from subjects positive for parasitological investigations.<h4<Results</h4<No HAT cases were detected among 345 people tested. A total of 167 pigs were investigated. Free-ranging pigs appeared significantly more infected than pigs in pen. Over 70% of free-ranging pigs were positive for CATT and parasitological investigations and 27-43% were positive to trypanolysis depending on the antigen used. T. brucei was the most prevalent species (57%) followed by T. congolense (24%). Blood sample extracted DNA of T. brucei positive subjects were negative to single round TgsGP PCR. However, 1/22 and 6/22 isolated strains were positive with single round and nested TgsGP PCRs, respectively.<h4<Discussion</h4<Free-ranging pigs were identified as a multi-reservoir of T. brucei and/or T. congolense with mixed infections of different strains. This trypanosome diversity hinders the easy and direct detection of T. b. gambiense. We highlight the lack of tools to prove or exclude with certainty the presence of T. b. gambiense. This study once more highlights the need of technical improvements to explore the role of animals in the epidemiology of HAT.
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container_issue	12, p e0010036
title_short	Free-ranging pigs identified as a multi-reservoir of Trypanosoma brucei and Trypanosoma congolense in the Vavoua area, a historical sleeping sickness focus of Côte d'Ivoire.
url	https://doi.org/10.1371/journal.pntd.0010036 https://doaj.org/article/73d9f15683d444ec86ccc2de38b236ca https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735
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author2	Mathurin Koffi Martial Kassi N'Djetchi Dramane Kaba Jacques Kaboré Hamidou Ilboudo Bernadin Ahouty Ahouty Minayégninrin Koné Bamoro Coulibaly Thomas Konan Adeline Segard Lingué Kouakou Thierry De Meeûs Sophie Ravel Philippe Solano Jean-Mathieu Bart Vincent Jamonneau
author2Str	Mathurin Koffi Martial Kassi N'Djetchi Dramane Kaba Jacques Kaboré Hamidou Ilboudo Bernadin Ahouty Ahouty Minayégninrin Koné Bamoro Coulibaly Thomas Konan Adeline Segard Lingué Kouakou Thierry De Meeûs Sophie Ravel Philippe Solano Jean-Mathieu Bart Vincent Jamonneau
ppnlink	568915356
callnumber-subject	RC - Internal Medicine
mediatype_str_mv	c
isOA_txt	true
hochschulschrift_bool	false
doi_str	10.1371/journal.pntd.0010036
callnumber-a	RC955-962
up_date	2024-07-03T22:30:54.077Z
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Free-ranging pigs identified as a multi-reservoir of Trypanosoma brucei and Trypanosoma congolense in the Vavoua area, a historical sleeping sickness focus of Côte d'Ivoire.

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