Acquired AmpC β-Lactamases among <i<Enterobacteriaceae</i< from Healthy Humans and Animals, Food, Aquatic and Trout Aquaculture Environments in Portugal
We aimed to investigate the occurrence of acquired AmpC β-lactamases (qAmpC), and characterize qAmpC-producing <i<Enterobacteriaceae</i< from different non-clinical environments in Portugal. We analysed 880 <i<Enterobacteriaceae</i< resistant to third-generation cephalosporin...
Ausführliche Beschreibung
Autor*in: |
Teresa Gonçalves Ribeiro [verfasserIn] Ângela Novais [verfasserIn] Elisabete Machado [verfasserIn] Luísa Peixe [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2020 |
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Schlagwörter: |
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Übergeordnetes Werk: |
In: Pathogens - MDPI AG, 2012, 9(2020), 4, p 273 |
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Übergeordnetes Werk: |
volume:9 ; year:2020 ; number:4, p 273 |
Links: |
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DOI / URN: |
10.3390/pathogens9040273 |
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Katalog-ID: |
DOAJ014386763 |
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10.3390/pathogens9040273 doi (DE-627)DOAJ014386763 (DE-599)DOAJ1d5ff8a869ef4809a1e0c8aa51382a9b DE-627 ger DE-627 rakwb eng Teresa Gonçalves Ribeiro verfasserin aut Acquired AmpC β-Lactamases among <i<Enterobacteriaceae</i< from Healthy Humans and Animals, Food, Aquatic and Trout Aquaculture Environments in Portugal 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We aimed to investigate the occurrence of acquired AmpC β-lactamases (qAmpC), and characterize qAmpC-producing <i<Enterobacteriaceae</i< from different non-clinical environments in Portugal. We analysed 880 <i<Enterobacteriaceae</i< resistant to third-generation cephalosporins recovered from 632 non-clinical samples [healthy human and healthy animal (swine, chickens) faeces; uncooked chicken carcasses; aquatic and trout aquaculture samples]. Bacterial and qAmpC identification, antibiotic susceptibility, clonal (PFGE, MLST) and plasmid (S1-/I-<i<Ceu</i<I-PFGE, replicon typing, hybridization) analysis were performed using standard methods. The occurrence of qAmpC among <i<Enterobacteriaceae</i< from non-clinical origins was low (0.6%; n = 4/628 samples), corresponding to CMY-2-producing <i<Escherichia coli</i< from three healthy humans (HH) and one uncooked chicken carcass (UCC). We highlight a slight increase in CMY-2 human faecal carriage in the two periods sampled [1.0% in 2013–2014 versus 0% in 2001–2004], which is in accordance with the trend observed in other European countries. CMY-2-producing <i<E. coli</i< belonged to B2<sub<2</sub<-ST4953 (n = 2, HH), A<sub<0</sub<-ST665 (n = 1, HH) or A<sub<1</sub<-ST48 (n = 1, UCC) clones. <i<bla</i<<sub<CMY-2</sub< was identified in non-typeable and IncA/C<sub<2</sub< plasmids. This study is one of the few providing an integrated evaluation of the qAmpC-producing <i<Enterobacteriaceae</i< occurrence, which was low, from a very large collection of different non-clinical origins. Further surveillance in contemporary collections can provide an integrated epidemiological information of potential shifts in reservoirs, transmission routes and mechanisms of dissemination of <i<bla</i<<sub<qAmpC</sub< in non-clinical settings. CMY-2 <i<Escherichia coli</i< ST48 ST665 plasmids Medicine R Ângela Novais verfasserin aut Elisabete Machado verfasserin aut Luísa Peixe verfasserin aut In Pathogens MDPI AG, 2012 9(2020), 4, p 273 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:9 year:2020 number:4, p 273 https://doi.org/10.3390/pathogens9040273 kostenfrei https://doaj.org/article/1d5ff8a869ef4809a1e0c8aa51382a9b kostenfrei https://www.mdpi.com/2076-0817/9/4/273 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 4, p 273 |
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10.3390/pathogens9040273 doi (DE-627)DOAJ014386763 (DE-599)DOAJ1d5ff8a869ef4809a1e0c8aa51382a9b DE-627 ger DE-627 rakwb eng Teresa Gonçalves Ribeiro verfasserin aut Acquired AmpC β-Lactamases among <i<Enterobacteriaceae</i< from Healthy Humans and Animals, Food, Aquatic and Trout Aquaculture Environments in Portugal 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We aimed to investigate the occurrence of acquired AmpC β-lactamases (qAmpC), and characterize qAmpC-producing <i<Enterobacteriaceae</i< from different non-clinical environments in Portugal. We analysed 880 <i<Enterobacteriaceae</i< resistant to third-generation cephalosporins recovered from 632 non-clinical samples [healthy human and healthy animal (swine, chickens) faeces; uncooked chicken carcasses; aquatic and trout aquaculture samples]. Bacterial and qAmpC identification, antibiotic susceptibility, clonal (PFGE, MLST) and plasmid (S1-/I-<i<Ceu</i<I-PFGE, replicon typing, hybridization) analysis were performed using standard methods. The occurrence of qAmpC among <i<Enterobacteriaceae</i< from non-clinical origins was low (0.6%; n = 4/628 samples), corresponding to CMY-2-producing <i<Escherichia coli</i< from three healthy humans (HH) and one uncooked chicken carcass (UCC). We highlight a slight increase in CMY-2 human faecal carriage in the two periods sampled [1.0% in 2013–2014 versus 0% in 2001–2004], which is in accordance with the trend observed in other European countries. CMY-2-producing <i<E. coli</i< belonged to B2<sub<2</sub<-ST4953 (n = 2, HH), A<sub<0</sub<-ST665 (n = 1, HH) or A<sub<1</sub<-ST48 (n = 1, UCC) clones. <i<bla</i<<sub<CMY-2</sub< was identified in non-typeable and IncA/C<sub<2</sub< plasmids. This study is one of the few providing an integrated evaluation of the qAmpC-producing <i<Enterobacteriaceae</i< occurrence, which was low, from a very large collection of different non-clinical origins. Further surveillance in contemporary collections can provide an integrated epidemiological information of potential shifts in reservoirs, transmission routes and mechanisms of dissemination of <i<bla</i<<sub<qAmpC</sub< in non-clinical settings. CMY-2 <i<Escherichia coli</i< ST48 ST665 plasmids Medicine R Ângela Novais verfasserin aut Elisabete Machado verfasserin aut Luísa Peixe verfasserin aut In Pathogens MDPI AG, 2012 9(2020), 4, p 273 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:9 year:2020 number:4, p 273 https://doi.org/10.3390/pathogens9040273 kostenfrei https://doaj.org/article/1d5ff8a869ef4809a1e0c8aa51382a9b kostenfrei https://www.mdpi.com/2076-0817/9/4/273 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 4, p 273 |
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10.3390/pathogens9040273 doi (DE-627)DOAJ014386763 (DE-599)DOAJ1d5ff8a869ef4809a1e0c8aa51382a9b DE-627 ger DE-627 rakwb eng Teresa Gonçalves Ribeiro verfasserin aut Acquired AmpC β-Lactamases among <i<Enterobacteriaceae</i< from Healthy Humans and Animals, Food, Aquatic and Trout Aquaculture Environments in Portugal 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We aimed to investigate the occurrence of acquired AmpC β-lactamases (qAmpC), and characterize qAmpC-producing <i<Enterobacteriaceae</i< from different non-clinical environments in Portugal. We analysed 880 <i<Enterobacteriaceae</i< resistant to third-generation cephalosporins recovered from 632 non-clinical samples [healthy human and healthy animal (swine, chickens) faeces; uncooked chicken carcasses; aquatic and trout aquaculture samples]. Bacterial and qAmpC identification, antibiotic susceptibility, clonal (PFGE, MLST) and plasmid (S1-/I-<i<Ceu</i<I-PFGE, replicon typing, hybridization) analysis were performed using standard methods. The occurrence of qAmpC among <i<Enterobacteriaceae</i< from non-clinical origins was low (0.6%; n = 4/628 samples), corresponding to CMY-2-producing <i<Escherichia coli</i< from three healthy humans (HH) and one uncooked chicken carcass (UCC). We highlight a slight increase in CMY-2 human faecal carriage in the two periods sampled [1.0% in 2013–2014 versus 0% in 2001–2004], which is in accordance with the trend observed in other European countries. CMY-2-producing <i<E. coli</i< belonged to B2<sub<2</sub<-ST4953 (n = 2, HH), A<sub<0</sub<-ST665 (n = 1, HH) or A<sub<1</sub<-ST48 (n = 1, UCC) clones. <i<bla</i<<sub<CMY-2</sub< was identified in non-typeable and IncA/C<sub<2</sub< plasmids. This study is one of the few providing an integrated evaluation of the qAmpC-producing <i<Enterobacteriaceae</i< occurrence, which was low, from a very large collection of different non-clinical origins. Further surveillance in contemporary collections can provide an integrated epidemiological information of potential shifts in reservoirs, transmission routes and mechanisms of dissemination of <i<bla</i<<sub<qAmpC</sub< in non-clinical settings. CMY-2 <i<Escherichia coli</i< ST48 ST665 plasmids Medicine R Ângela Novais verfasserin aut Elisabete Machado verfasserin aut Luísa Peixe verfasserin aut In Pathogens MDPI AG, 2012 9(2020), 4, p 273 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:9 year:2020 number:4, p 273 https://doi.org/10.3390/pathogens9040273 kostenfrei https://doaj.org/article/1d5ff8a869ef4809a1e0c8aa51382a9b kostenfrei https://www.mdpi.com/2076-0817/9/4/273 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 4, p 273 |
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10.3390/pathogens9040273 doi (DE-627)DOAJ014386763 (DE-599)DOAJ1d5ff8a869ef4809a1e0c8aa51382a9b DE-627 ger DE-627 rakwb eng Teresa Gonçalves Ribeiro verfasserin aut Acquired AmpC β-Lactamases among <i<Enterobacteriaceae</i< from Healthy Humans and Animals, Food, Aquatic and Trout Aquaculture Environments in Portugal 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We aimed to investigate the occurrence of acquired AmpC β-lactamases (qAmpC), and characterize qAmpC-producing <i<Enterobacteriaceae</i< from different non-clinical environments in Portugal. We analysed 880 <i<Enterobacteriaceae</i< resistant to third-generation cephalosporins recovered from 632 non-clinical samples [healthy human and healthy animal (swine, chickens) faeces; uncooked chicken carcasses; aquatic and trout aquaculture samples]. Bacterial and qAmpC identification, antibiotic susceptibility, clonal (PFGE, MLST) and plasmid (S1-/I-<i<Ceu</i<I-PFGE, replicon typing, hybridization) analysis were performed using standard methods. The occurrence of qAmpC among <i<Enterobacteriaceae</i< from non-clinical origins was low (0.6%; n = 4/628 samples), corresponding to CMY-2-producing <i<Escherichia coli</i< from three healthy humans (HH) and one uncooked chicken carcass (UCC). We highlight a slight increase in CMY-2 human faecal carriage in the two periods sampled [1.0% in 2013–2014 versus 0% in 2001–2004], which is in accordance with the trend observed in other European countries. CMY-2-producing <i<E. coli</i< belonged to B2<sub<2</sub<-ST4953 (n = 2, HH), A<sub<0</sub<-ST665 (n = 1, HH) or A<sub<1</sub<-ST48 (n = 1, UCC) clones. <i<bla</i<<sub<CMY-2</sub< was identified in non-typeable and IncA/C<sub<2</sub< plasmids. This study is one of the few providing an integrated evaluation of the qAmpC-producing <i<Enterobacteriaceae</i< occurrence, which was low, from a very large collection of different non-clinical origins. Further surveillance in contemporary collections can provide an integrated epidemiological information of potential shifts in reservoirs, transmission routes and mechanisms of dissemination of <i<bla</i<<sub<qAmpC</sub< in non-clinical settings. CMY-2 <i<Escherichia coli</i< ST48 ST665 plasmids Medicine R Ângela Novais verfasserin aut Elisabete Machado verfasserin aut Luísa Peixe verfasserin aut In Pathogens MDPI AG, 2012 9(2020), 4, p 273 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:9 year:2020 number:4, p 273 https://doi.org/10.3390/pathogens9040273 kostenfrei https://doaj.org/article/1d5ff8a869ef4809a1e0c8aa51382a9b kostenfrei https://www.mdpi.com/2076-0817/9/4/273 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 4, p 273 |
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10.3390/pathogens9040273 doi (DE-627)DOAJ014386763 (DE-599)DOAJ1d5ff8a869ef4809a1e0c8aa51382a9b DE-627 ger DE-627 rakwb eng Teresa Gonçalves Ribeiro verfasserin aut Acquired AmpC β-Lactamases among <i<Enterobacteriaceae</i< from Healthy Humans and Animals, Food, Aquatic and Trout Aquaculture Environments in Portugal 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We aimed to investigate the occurrence of acquired AmpC β-lactamases (qAmpC), and characterize qAmpC-producing <i<Enterobacteriaceae</i< from different non-clinical environments in Portugal. We analysed 880 <i<Enterobacteriaceae</i< resistant to third-generation cephalosporins recovered from 632 non-clinical samples [healthy human and healthy animal (swine, chickens) faeces; uncooked chicken carcasses; aquatic and trout aquaculture samples]. Bacterial and qAmpC identification, antibiotic susceptibility, clonal (PFGE, MLST) and plasmid (S1-/I-<i<Ceu</i<I-PFGE, replicon typing, hybridization) analysis were performed using standard methods. The occurrence of qAmpC among <i<Enterobacteriaceae</i< from non-clinical origins was low (0.6%; n = 4/628 samples), corresponding to CMY-2-producing <i<Escherichia coli</i< from three healthy humans (HH) and one uncooked chicken carcass (UCC). We highlight a slight increase in CMY-2 human faecal carriage in the two periods sampled [1.0% in 2013–2014 versus 0% in 2001–2004], which is in accordance with the trend observed in other European countries. CMY-2-producing <i<E. coli</i< belonged to B2<sub<2</sub<-ST4953 (n = 2, HH), A<sub<0</sub<-ST665 (n = 1, HH) or A<sub<1</sub<-ST48 (n = 1, UCC) clones. <i<bla</i<<sub<CMY-2</sub< was identified in non-typeable and IncA/C<sub<2</sub< plasmids. This study is one of the few providing an integrated evaluation of the qAmpC-producing <i<Enterobacteriaceae</i< occurrence, which was low, from a very large collection of different non-clinical origins. Further surveillance in contemporary collections can provide an integrated epidemiological information of potential shifts in reservoirs, transmission routes and mechanisms of dissemination of <i<bla</i<<sub<qAmpC</sub< in non-clinical settings. CMY-2 <i<Escherichia coli</i< ST48 ST665 plasmids Medicine R Ângela Novais verfasserin aut Elisabete Machado verfasserin aut Luísa Peixe verfasserin aut In Pathogens MDPI AG, 2012 9(2020), 4, p 273 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:9 year:2020 number:4, p 273 https://doi.org/10.3390/pathogens9040273 kostenfrei https://doaj.org/article/1d5ff8a869ef4809a1e0c8aa51382a9b kostenfrei https://www.mdpi.com/2076-0817/9/4/273 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 4, p 273 |
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Acquired AmpC β-Lactamases among <i<Enterobacteriaceae</i< from Healthy Humans and Animals, Food, Aquatic and Trout Aquaculture Environments in Portugal |
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We aimed to investigate the occurrence of acquired AmpC β-lactamases (qAmpC), and characterize qAmpC-producing <i<Enterobacteriaceae</i< from different non-clinical environments in Portugal. We analysed 880 <i<Enterobacteriaceae</i< resistant to third-generation cephalosporins recovered from 632 non-clinical samples [healthy human and healthy animal (swine, chickens) faeces; uncooked chicken carcasses; aquatic and trout aquaculture samples]. Bacterial and qAmpC identification, antibiotic susceptibility, clonal (PFGE, MLST) and plasmid (S1-/I-<i<Ceu</i<I-PFGE, replicon typing, hybridization) analysis were performed using standard methods. The occurrence of qAmpC among <i<Enterobacteriaceae</i< from non-clinical origins was low (0.6%; n = 4/628 samples), corresponding to CMY-2-producing <i<Escherichia coli</i< from three healthy humans (HH) and one uncooked chicken carcass (UCC). We highlight a slight increase in CMY-2 human faecal carriage in the two periods sampled [1.0% in 2013–2014 versus 0% in 2001–2004], which is in accordance with the trend observed in other European countries. CMY-2-producing <i<E. coli</i< belonged to B2<sub<2</sub<-ST4953 (n = 2, HH), A<sub<0</sub<-ST665 (n = 1, HH) or A<sub<1</sub<-ST48 (n = 1, UCC) clones. <i<bla</i<<sub<CMY-2</sub< was identified in non-typeable and IncA/C<sub<2</sub< plasmids. This study is one of the few providing an integrated evaluation of the qAmpC-producing <i<Enterobacteriaceae</i< occurrence, which was low, from a very large collection of different non-clinical origins. Further surveillance in contemporary collections can provide an integrated epidemiological information of potential shifts in reservoirs, transmission routes and mechanisms of dissemination of <i<bla</i<<sub<qAmpC</sub< in non-clinical settings. |
abstractGer |
We aimed to investigate the occurrence of acquired AmpC β-lactamases (qAmpC), and characterize qAmpC-producing <i<Enterobacteriaceae</i< from different non-clinical environments in Portugal. We analysed 880 <i<Enterobacteriaceae</i< resistant to third-generation cephalosporins recovered from 632 non-clinical samples [healthy human and healthy animal (swine, chickens) faeces; uncooked chicken carcasses; aquatic and trout aquaculture samples]. Bacterial and qAmpC identification, antibiotic susceptibility, clonal (PFGE, MLST) and plasmid (S1-/I-<i<Ceu</i<I-PFGE, replicon typing, hybridization) analysis were performed using standard methods. The occurrence of qAmpC among <i<Enterobacteriaceae</i< from non-clinical origins was low (0.6%; n = 4/628 samples), corresponding to CMY-2-producing <i<Escherichia coli</i< from three healthy humans (HH) and one uncooked chicken carcass (UCC). We highlight a slight increase in CMY-2 human faecal carriage in the two periods sampled [1.0% in 2013–2014 versus 0% in 2001–2004], which is in accordance with the trend observed in other European countries. CMY-2-producing <i<E. coli</i< belonged to B2<sub<2</sub<-ST4953 (n = 2, HH), A<sub<0</sub<-ST665 (n = 1, HH) or A<sub<1</sub<-ST48 (n = 1, UCC) clones. <i<bla</i<<sub<CMY-2</sub< was identified in non-typeable and IncA/C<sub<2</sub< plasmids. This study is one of the few providing an integrated evaluation of the qAmpC-producing <i<Enterobacteriaceae</i< occurrence, which was low, from a very large collection of different non-clinical origins. Further surveillance in contemporary collections can provide an integrated epidemiological information of potential shifts in reservoirs, transmission routes and mechanisms of dissemination of <i<bla</i<<sub<qAmpC</sub< in non-clinical settings. |
abstract_unstemmed |
We aimed to investigate the occurrence of acquired AmpC β-lactamases (qAmpC), and characterize qAmpC-producing <i<Enterobacteriaceae</i< from different non-clinical environments in Portugal. We analysed 880 <i<Enterobacteriaceae</i< resistant to third-generation cephalosporins recovered from 632 non-clinical samples [healthy human and healthy animal (swine, chickens) faeces; uncooked chicken carcasses; aquatic and trout aquaculture samples]. Bacterial and qAmpC identification, antibiotic susceptibility, clonal (PFGE, MLST) and plasmid (S1-/I-<i<Ceu</i<I-PFGE, replicon typing, hybridization) analysis were performed using standard methods. The occurrence of qAmpC among <i<Enterobacteriaceae</i< from non-clinical origins was low (0.6%; n = 4/628 samples), corresponding to CMY-2-producing <i<Escherichia coli</i< from three healthy humans (HH) and one uncooked chicken carcass (UCC). We highlight a slight increase in CMY-2 human faecal carriage in the two periods sampled [1.0% in 2013–2014 versus 0% in 2001–2004], which is in accordance with the trend observed in other European countries. CMY-2-producing <i<E. coli</i< belonged to B2<sub<2</sub<-ST4953 (n = 2, HH), A<sub<0</sub<-ST665 (n = 1, HH) or A<sub<1</sub<-ST48 (n = 1, UCC) clones. <i<bla</i<<sub<CMY-2</sub< was identified in non-typeable and IncA/C<sub<2</sub< plasmids. This study is one of the few providing an integrated evaluation of the qAmpC-producing <i<Enterobacteriaceae</i< occurrence, which was low, from a very large collection of different non-clinical origins. Further surveillance in contemporary collections can provide an integrated epidemiological information of potential shifts in reservoirs, transmission routes and mechanisms of dissemination of <i<bla</i<<sub<qAmpC</sub< in non-clinical settings. |
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4, p 273 |
title_short |
Acquired AmpC β-Lactamases among <i<Enterobacteriaceae</i< from Healthy Humans and Animals, Food, Aquatic and Trout Aquaculture Environments in Portugal |
url |
https://doi.org/10.3390/pathogens9040273 https://doaj.org/article/1d5ff8a869ef4809a1e0c8aa51382a9b https://www.mdpi.com/2076-0817/9/4/273 https://doaj.org/toc/2076-0817 |
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Ângela Novais Elisabete Machado Luísa Peixe |
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