Development of a Dendrimeric Peptide-Based Approach for the Differentiation of Animals Vaccinated with FlagT4G against Classical Swine Fever from Infected Pigs

Classical swine fever virus (CSFV) causes a viral disease of high epidemiological and economical significance that affects domestic and wild swine. Control of the disease in endemic countries is based on live-attenuated vaccines (LAVs) that induce an early protective immune response against highly v...
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Autor*in:	José Alejandro Bohórquez [verfasserIn] Sira Defaus [verfasserIn] Rosa Rosell [verfasserIn] Marta Pérez-Simó [verfasserIn] Mònica Alberch [verfasserIn] Douglas P. Gladue [verfasserIn] Manuel V. Borca [verfasserIn] David Andreu [verfasserIn] Llilianne Ganges [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	CSFV live-attenuated vaccine serological DIVA test FlagT4G CSFV vaccine candidate

Übergeordnetes Werk:	In: Viruses - MDPI AG, 2009, 13(2021), 10, p 1980
Übergeordnetes Werk:	volume:13 ; year:2021 ; number:10, p 1980

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/v13101980

Katalog-ID:	DOAJ014467313

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allfields	10.3390/v13101980 doi (DE-627)DOAJ014467313 (DE-599)DOAJf54ceaeee7264f849e3c3c3a460955f3 DE-627 ger DE-627 rakwb eng QR1-502 José Alejandro Bohórquez verfasserin aut Development of a Dendrimeric Peptide-Based Approach for the Differentiation of Animals Vaccinated with FlagT4G against Classical Swine Fever from Infected Pigs 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Classical swine fever virus (CSFV) causes a viral disease of high epidemiological and economical significance that affects domestic and wild swine. Control of the disease in endemic countries is based on live-attenuated vaccines (LAVs) that induce an early protective immune response against highly virulent CSFV strains. The main disadvantage of these currently available LAVs is the lack of serological techniques to differentiate between vaccinated and infected animals (DIVA concept). Here, we describe the development of the FlagDIVA test, a serological diagnostic tool allowing for the differentiation between animals vaccinated with the FlagT4G candidate and those infected with CSFV field strains. The FlagDIVA test is a direct ELISA based on a dendrimeric peptide construct displaying a conserved epitope of CSFV structural protein E2. Although FlagDIVA detected anti-CSFV anti-bodies in infected animals, it did not recognize the antibody response of FlagT4G-vaccinated animals. Therefore, the FlagDIVA test constitutes a valuable accessory DIVA tool in implementing vaccination with the FlagT4G candidate. CSFV live-attenuated vaccine serological DIVA test FlagT4G CSFV vaccine candidate Microbiology Sira Defaus verfasserin aut Rosa Rosell verfasserin aut Marta Pérez-Simó verfasserin aut Mònica Alberch verfasserin aut Douglas P. Gladue verfasserin aut Manuel V. Borca verfasserin aut David Andreu verfasserin aut Llilianne Ganges verfasserin aut In Viruses MDPI AG, 2009 13(2021), 10, p 1980 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:13 year:2021 number:10, p 1980 https://doi.org/10.3390/v13101980 kostenfrei https://doaj.org/article/f54ceaeee7264f849e3c3c3a460955f3 kostenfrei https://www.mdpi.com/1999-4915/13/10/1980 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2021 10, p 1980
spelling	10.3390/v13101980 doi (DE-627)DOAJ014467313 (DE-599)DOAJf54ceaeee7264f849e3c3c3a460955f3 DE-627 ger DE-627 rakwb eng QR1-502 José Alejandro Bohórquez verfasserin aut Development of a Dendrimeric Peptide-Based Approach for the Differentiation of Animals Vaccinated with FlagT4G against Classical Swine Fever from Infected Pigs 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Classical swine fever virus (CSFV) causes a viral disease of high epidemiological and economical significance that affects domestic and wild swine. Control of the disease in endemic countries is based on live-attenuated vaccines (LAVs) that induce an early protective immune response against highly virulent CSFV strains. The main disadvantage of these currently available LAVs is the lack of serological techniques to differentiate between vaccinated and infected animals (DIVA concept). Here, we describe the development of the FlagDIVA test, a serological diagnostic tool allowing for the differentiation between animals vaccinated with the FlagT4G candidate and those infected with CSFV field strains. The FlagDIVA test is a direct ELISA based on a dendrimeric peptide construct displaying a conserved epitope of CSFV structural protein E2. Although FlagDIVA detected anti-CSFV anti-bodies in infected animals, it did not recognize the antibody response of FlagT4G-vaccinated animals. Therefore, the FlagDIVA test constitutes a valuable accessory DIVA tool in implementing vaccination with the FlagT4G candidate. CSFV live-attenuated vaccine serological DIVA test FlagT4G CSFV vaccine candidate Microbiology Sira Defaus verfasserin aut Rosa Rosell verfasserin aut Marta Pérez-Simó verfasserin aut Mònica Alberch verfasserin aut Douglas P. Gladue verfasserin aut Manuel V. Borca verfasserin aut David Andreu verfasserin aut Llilianne Ganges verfasserin aut In Viruses MDPI AG, 2009 13(2021), 10, p 1980 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:13 year:2021 number:10, p 1980 https://doi.org/10.3390/v13101980 kostenfrei https://doaj.org/article/f54ceaeee7264f849e3c3c3a460955f3 kostenfrei https://www.mdpi.com/1999-4915/13/10/1980 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2021 10, p 1980
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allfieldsGer	10.3390/v13101980 doi (DE-627)DOAJ014467313 (DE-599)DOAJf54ceaeee7264f849e3c3c3a460955f3 DE-627 ger DE-627 rakwb eng QR1-502 José Alejandro Bohórquez verfasserin aut Development of a Dendrimeric Peptide-Based Approach for the Differentiation of Animals Vaccinated with FlagT4G against Classical Swine Fever from Infected Pigs 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Classical swine fever virus (CSFV) causes a viral disease of high epidemiological and economical significance that affects domestic and wild swine. Control of the disease in endemic countries is based on live-attenuated vaccines (LAVs) that induce an early protective immune response against highly virulent CSFV strains. The main disadvantage of these currently available LAVs is the lack of serological techniques to differentiate between vaccinated and infected animals (DIVA concept). Here, we describe the development of the FlagDIVA test, a serological diagnostic tool allowing for the differentiation between animals vaccinated with the FlagT4G candidate and those infected with CSFV field strains. The FlagDIVA test is a direct ELISA based on a dendrimeric peptide construct displaying a conserved epitope of CSFV structural protein E2. Although FlagDIVA detected anti-CSFV anti-bodies in infected animals, it did not recognize the antibody response of FlagT4G-vaccinated animals. Therefore, the FlagDIVA test constitutes a valuable accessory DIVA tool in implementing vaccination with the FlagT4G candidate. CSFV live-attenuated vaccine serological DIVA test FlagT4G CSFV vaccine candidate Microbiology Sira Defaus verfasserin aut Rosa Rosell verfasserin aut Marta Pérez-Simó verfasserin aut Mònica Alberch verfasserin aut Douglas P. Gladue verfasserin aut Manuel V. Borca verfasserin aut David Andreu verfasserin aut Llilianne Ganges verfasserin aut In Viruses MDPI AG, 2009 13(2021), 10, p 1980 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:13 year:2021 number:10, p 1980 https://doi.org/10.3390/v13101980 kostenfrei https://doaj.org/article/f54ceaeee7264f849e3c3c3a460955f3 kostenfrei https://www.mdpi.com/1999-4915/13/10/1980 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2021 10, p 1980
allfieldsSound	10.3390/v13101980 doi (DE-627)DOAJ014467313 (DE-599)DOAJf54ceaeee7264f849e3c3c3a460955f3 DE-627 ger DE-627 rakwb eng QR1-502 José Alejandro Bohórquez verfasserin aut Development of a Dendrimeric Peptide-Based Approach for the Differentiation of Animals Vaccinated with FlagT4G against Classical Swine Fever from Infected Pigs 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Classical swine fever virus (CSFV) causes a viral disease of high epidemiological and economical significance that affects domestic and wild swine. Control of the disease in endemic countries is based on live-attenuated vaccines (LAVs) that induce an early protective immune response against highly virulent CSFV strains. The main disadvantage of these currently available LAVs is the lack of serological techniques to differentiate between vaccinated and infected animals (DIVA concept). Here, we describe the development of the FlagDIVA test, a serological diagnostic tool allowing for the differentiation between animals vaccinated with the FlagT4G candidate and those infected with CSFV field strains. The FlagDIVA test is a direct ELISA based on a dendrimeric peptide construct displaying a conserved epitope of CSFV structural protein E2. Although FlagDIVA detected anti-CSFV anti-bodies in infected animals, it did not recognize the antibody response of FlagT4G-vaccinated animals. Therefore, the FlagDIVA test constitutes a valuable accessory DIVA tool in implementing vaccination with the FlagT4G candidate. CSFV live-attenuated vaccine serological DIVA test FlagT4G CSFV vaccine candidate Microbiology Sira Defaus verfasserin aut Rosa Rosell verfasserin aut Marta Pérez-Simó verfasserin aut Mònica Alberch verfasserin aut Douglas P. Gladue verfasserin aut Manuel V. Borca verfasserin aut David Andreu verfasserin aut Llilianne Ganges verfasserin aut In Viruses MDPI AG, 2009 13(2021), 10, p 1980 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:13 year:2021 number:10, p 1980 https://doi.org/10.3390/v13101980 kostenfrei https://doaj.org/article/f54ceaeee7264f849e3c3c3a460955f3 kostenfrei https://www.mdpi.com/1999-4915/13/10/1980 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2021 10, p 1980
language	English
source	In Viruses 13(2021), 10, p 1980 volume:13 year:2021 number:10, p 1980
sourceStr	In Viruses 13(2021), 10, p 1980 volume:13 year:2021 number:10, p 1980
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	CSFV live-attenuated vaccine serological DIVA test FlagT4G CSFV vaccine candidate Microbiology
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container_title	Viruses
authorswithroles_txt_mv	José Alejandro Bohórquez @@aut@@ Sira Defaus @@aut@@ Rosa Rosell @@aut@@ Marta Pérez-Simó @@aut@@ Mònica Alberch @@aut@@ Douglas P. Gladue @@aut@@ Manuel V. Borca @@aut@@ David Andreu @@aut@@ Llilianne Ganges @@aut@@
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callnumber-first	Q - Science
author	José Alejandro Bohórquez
spellingShingle	José Alejandro Bohórquez misc QR1-502 misc CSFV live-attenuated vaccine misc serological DIVA test misc FlagT4G CSFV vaccine candidate misc Microbiology Development of a Dendrimeric Peptide-Based Approach for the Differentiation of Animals Vaccinated with FlagT4G against Classical Swine Fever from Infected Pigs
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topic_title	QR1-502 Development of a Dendrimeric Peptide-Based Approach for the Differentiation of Animals Vaccinated with FlagT4G against Classical Swine Fever from Infected Pigs CSFV live-attenuated vaccine serological DIVA test FlagT4G CSFV vaccine candidate
topic	misc QR1-502 misc CSFV live-attenuated vaccine misc serological DIVA test misc FlagT4G CSFV vaccine candidate misc Microbiology
topic_unstemmed	misc QR1-502 misc CSFV live-attenuated vaccine misc serological DIVA test misc FlagT4G CSFV vaccine candidate misc Microbiology
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title	Development of a Dendrimeric Peptide-Based Approach for the Differentiation of Animals Vaccinated with FlagT4G against Classical Swine Fever from Infected Pigs
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title_full	Development of a Dendrimeric Peptide-Based Approach for the Differentiation of Animals Vaccinated with FlagT4G against Classical Swine Fever from Infected Pigs
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author_browse	José Alejandro Bohórquez Sira Defaus Rosa Rosell Marta Pérez-Simó Mònica Alberch Douglas P. Gladue Manuel V. Borca David Andreu Llilianne Ganges
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title_sort	development of a dendrimeric peptide-based approach for the differentiation of animals vaccinated with flagt4g against classical swine fever from infected pigs
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title_auth	Development of a Dendrimeric Peptide-Based Approach for the Differentiation of Animals Vaccinated with FlagT4G against Classical Swine Fever from Infected Pigs
abstract	Classical swine fever virus (CSFV) causes a viral disease of high epidemiological and economical significance that affects domestic and wild swine. Control of the disease in endemic countries is based on live-attenuated vaccines (LAVs) that induce an early protective immune response against highly virulent CSFV strains. The main disadvantage of these currently available LAVs is the lack of serological techniques to differentiate between vaccinated and infected animals (DIVA concept). Here, we describe the development of the FlagDIVA test, a serological diagnostic tool allowing for the differentiation between animals vaccinated with the FlagT4G candidate and those infected with CSFV field strains. The FlagDIVA test is a direct ELISA based on a dendrimeric peptide construct displaying a conserved epitope of CSFV structural protein E2. Although FlagDIVA detected anti-CSFV anti-bodies in infected animals, it did not recognize the antibody response of FlagT4G-vaccinated animals. Therefore, the FlagDIVA test constitutes a valuable accessory DIVA tool in implementing vaccination with the FlagT4G candidate.
abstractGer	Classical swine fever virus (CSFV) causes a viral disease of high epidemiological and economical significance that affects domestic and wild swine. Control of the disease in endemic countries is based on live-attenuated vaccines (LAVs) that induce an early protective immune response against highly virulent CSFV strains. The main disadvantage of these currently available LAVs is the lack of serological techniques to differentiate between vaccinated and infected animals (DIVA concept). Here, we describe the development of the FlagDIVA test, a serological diagnostic tool allowing for the differentiation between animals vaccinated with the FlagT4G candidate and those infected with CSFV field strains. The FlagDIVA test is a direct ELISA based on a dendrimeric peptide construct displaying a conserved epitope of CSFV structural protein E2. Although FlagDIVA detected anti-CSFV anti-bodies in infected animals, it did not recognize the antibody response of FlagT4G-vaccinated animals. Therefore, the FlagDIVA test constitutes a valuable accessory DIVA tool in implementing vaccination with the FlagT4G candidate.
abstract_unstemmed	Classical swine fever virus (CSFV) causes a viral disease of high epidemiological and economical significance that affects domestic and wild swine. Control of the disease in endemic countries is based on live-attenuated vaccines (LAVs) that induce an early protective immune response against highly virulent CSFV strains. The main disadvantage of these currently available LAVs is the lack of serological techniques to differentiate between vaccinated and infected animals (DIVA concept). Here, we describe the development of the FlagDIVA test, a serological diagnostic tool allowing for the differentiation between animals vaccinated with the FlagT4G candidate and those infected with CSFV field strains. The FlagDIVA test is a direct ELISA based on a dendrimeric peptide construct displaying a conserved epitope of CSFV structural protein E2. Although FlagDIVA detected anti-CSFV anti-bodies in infected animals, it did not recognize the antibody response of FlagT4G-vaccinated animals. Therefore, the FlagDIVA test constitutes a valuable accessory DIVA tool in implementing vaccination with the FlagT4G candidate.
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container_issue	10, p 1980
title_short	Development of a Dendrimeric Peptide-Based Approach for the Differentiation of Animals Vaccinated with FlagT4G against Classical Swine Fever from Infected Pigs
url	https://doi.org/10.3390/v13101980 https://doaj.org/article/f54ceaeee7264f849e3c3c3a460955f3 https://www.mdpi.com/1999-4915/13/10/1980 https://doaj.org/toc/1999-4915
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up_date	2024-07-03T23:09:35.466Z
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Development of a Dendrimeric Peptide-Based Approach for the Differentiation of Animals Vaccinated with FlagT4G against Classical Swine Fever from Infected Pigs

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