Utilization of Glycine by Microorganisms along the Altitude Changbai Mountain, China: An Uptake Test Using <sup<13</sup<C,<sup<15</sup<N Labeling and <sup<13</sup<C-PLFA Analysis

External organic nitrogen (N) inputs can contrastingly affect the transformation and availability of N in forest soils, which is an important potential N resource and is possibly vulnerable to soil properties. Little is known about the transformation and availability of external small molecule organ...
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Autor*in:	Yan Xue [verfasserIn] Zhijie Wu [verfasserIn] Lili Zhang [verfasserIn] Wei Bai [verfasserIn] Dongpo Li [verfasserIn] Lijie Yang [verfasserIn] Ping Gong [verfasserIn] Zhanbo Wei [verfasserIn] Yuchao Song [verfasserIn] Lei Cui [verfasserIn] Kaikuo Wu [verfasserIn] Furong Xiao [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	glycine organic N uptake soil microorganisms PLFA Changbai Mountain

Übergeordnetes Werk:	In: Forests - MDPI AG, 2010, 13(2022), 2, p 307
Übergeordnetes Werk:	volume:13 ; year:2022 ; number:2, p 307

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/f13020307

Katalog-ID:	DOAJ014476835

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allfields	10.3390/f13020307 doi (DE-627)DOAJ014476835 (DE-599)DOAJ0f568bd4d0894a1dab6eef842055571f DE-627 ger DE-627 rakwb eng QK900-989 Yan Xue verfasserin aut Utilization of Glycine by Microorganisms along the Altitude Changbai Mountain, China: An Uptake Test Using <sup<13</sup<C,<sup<15</sup<N Labeling and <sup<13</sup<C-PLFA Analysis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier External organic nitrogen (N) inputs can contrastingly affect the transformation and availability of N in forest soils, which is an important potential N resource and is possibly vulnerable to soil properties. Little is known about the transformation and availability of external small molecule organic N in forest soils and the underlying microbial mechanisms. Soil samples from Changbai Mountain at different altitudes (from 750 m to 2200 m) that ranged widely in soil properties were incubated with <sup<13</sup<C, <sup<15</sup<N-labeled glycine. The fate of <sup<15</sup<N-glycine and the incorporation of <sup<13</sup<C into different phospholipid fatty acids (PLFAs) were measured at the same time. The addition of glycine promoted gross N mineralization and microbial N immobilization significantly. Mineralization of glycine N accounted for 6.2–22.5% of the added glycine and can be explicable in the light of a readily mineralizable substrate by soil microorganisms. Assimilation of glycine N into microbial biomass by the mineralization-immobilization-turnover (MIT) route accounted for 24.7–52.1% of the added label and was most mightily affected by the soil C/N ratio. We also found that the direct utilization of glycine is important to fulfill microorganism growth under the lack of available carbon (C) at upper elevations. The labeled glycine was rapidly incorporated into the PLFAs and was primarily assimilated by bacteria, indicating that different groups of the microbial community were answerable to external organic N. G+ bacteria were the main competitors for the exogenous glycine. Increased intact incorporation of glycine into microbial biomass and the concentration of PLFAs in general, particularly in G+ bacteria, suggest a diversified arrangement to response changes in substrate availability. glycine organic N uptake soil microorganisms PLFA Changbai Mountain Plant ecology Zhijie Wu verfasserin aut Lili Zhang verfasserin aut Wei Bai verfasserin aut Dongpo Li verfasserin aut Lijie Yang verfasserin aut Ping Gong verfasserin aut Zhanbo Wei verfasserin aut Yuchao Song verfasserin aut Lei Cui verfasserin aut Kaikuo Wu verfasserin aut Furong Xiao verfasserin aut In Forests MDPI AG, 2010 13(2022), 2, p 307 (DE-627)614095689 (DE-600)2527081-3 19994907 nnns volume:13 year:2022 number:2, p 307 https://doi.org/10.3390/f13020307 kostenfrei https://doaj.org/article/0f568bd4d0894a1dab6eef842055571f kostenfrei https://www.mdpi.com/1999-4907/13/2/307 kostenfrei https://doaj.org/toc/1999-4907 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 2, p 307
spelling	10.3390/f13020307 doi (DE-627)DOAJ014476835 (DE-599)DOAJ0f568bd4d0894a1dab6eef842055571f DE-627 ger DE-627 rakwb eng QK900-989 Yan Xue verfasserin aut Utilization of Glycine by Microorganisms along the Altitude Changbai Mountain, China: An Uptake Test Using <sup<13</sup<C,<sup<15</sup<N Labeling and <sup<13</sup<C-PLFA Analysis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier External organic nitrogen (N) inputs can contrastingly affect the transformation and availability of N in forest soils, which is an important potential N resource and is possibly vulnerable to soil properties. Little is known about the transformation and availability of external small molecule organic N in forest soils and the underlying microbial mechanisms. Soil samples from Changbai Mountain at different altitudes (from 750 m to 2200 m) that ranged widely in soil properties were incubated with <sup<13</sup<C, <sup<15</sup<N-labeled glycine. The fate of <sup<15</sup<N-glycine and the incorporation of <sup<13</sup<C into different phospholipid fatty acids (PLFAs) were measured at the same time. The addition of glycine promoted gross N mineralization and microbial N immobilization significantly. Mineralization of glycine N accounted for 6.2–22.5% of the added glycine and can be explicable in the light of a readily mineralizable substrate by soil microorganisms. Assimilation of glycine N into microbial biomass by the mineralization-immobilization-turnover (MIT) route accounted for 24.7–52.1% of the added label and was most mightily affected by the soil C/N ratio. We also found that the direct utilization of glycine is important to fulfill microorganism growth under the lack of available carbon (C) at upper elevations. The labeled glycine was rapidly incorporated into the PLFAs and was primarily assimilated by bacteria, indicating that different groups of the microbial community were answerable to external organic N. G+ bacteria were the main competitors for the exogenous glycine. Increased intact incorporation of glycine into microbial biomass and the concentration of PLFAs in general, particularly in G+ bacteria, suggest a diversified arrangement to response changes in substrate availability. glycine organic N uptake soil microorganisms PLFA Changbai Mountain Plant ecology Zhijie Wu verfasserin aut Lili Zhang verfasserin aut Wei Bai verfasserin aut Dongpo Li verfasserin aut Lijie Yang verfasserin aut Ping Gong verfasserin aut Zhanbo Wei verfasserin aut Yuchao Song verfasserin aut Lei Cui verfasserin aut Kaikuo Wu verfasserin aut Furong Xiao verfasserin aut In Forests MDPI AG, 2010 13(2022), 2, p 307 (DE-627)614095689 (DE-600)2527081-3 19994907 nnns volume:13 year:2022 number:2, p 307 https://doi.org/10.3390/f13020307 kostenfrei https://doaj.org/article/0f568bd4d0894a1dab6eef842055571f kostenfrei https://www.mdpi.com/1999-4907/13/2/307 kostenfrei https://doaj.org/toc/1999-4907 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 2, p 307
allfields_unstemmed	10.3390/f13020307 doi (DE-627)DOAJ014476835 (DE-599)DOAJ0f568bd4d0894a1dab6eef842055571f DE-627 ger DE-627 rakwb eng QK900-989 Yan Xue verfasserin aut Utilization of Glycine by Microorganisms along the Altitude Changbai Mountain, China: An Uptake Test Using <sup<13</sup<C,<sup<15</sup<N Labeling and <sup<13</sup<C-PLFA Analysis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier External organic nitrogen (N) inputs can contrastingly affect the transformation and availability of N in forest soils, which is an important potential N resource and is possibly vulnerable to soil properties. Little is known about the transformation and availability of external small molecule organic N in forest soils and the underlying microbial mechanisms. Soil samples from Changbai Mountain at different altitudes (from 750 m to 2200 m) that ranged widely in soil properties were incubated with <sup<13</sup<C, <sup<15</sup<N-labeled glycine. The fate of <sup<15</sup<N-glycine and the incorporation of <sup<13</sup<C into different phospholipid fatty acids (PLFAs) were measured at the same time. The addition of glycine promoted gross N mineralization and microbial N immobilization significantly. Mineralization of glycine N accounted for 6.2–22.5% of the added glycine and can be explicable in the light of a readily mineralizable substrate by soil microorganisms. Assimilation of glycine N into microbial biomass by the mineralization-immobilization-turnover (MIT) route accounted for 24.7–52.1% of the added label and was most mightily affected by the soil C/N ratio. We also found that the direct utilization of glycine is important to fulfill microorganism growth under the lack of available carbon (C) at upper elevations. The labeled glycine was rapidly incorporated into the PLFAs and was primarily assimilated by bacteria, indicating that different groups of the microbial community were answerable to external organic N. G+ bacteria were the main competitors for the exogenous glycine. Increased intact incorporation of glycine into microbial biomass and the concentration of PLFAs in general, particularly in G+ bacteria, suggest a diversified arrangement to response changes in substrate availability. glycine organic N uptake soil microorganisms PLFA Changbai Mountain Plant ecology Zhijie Wu verfasserin aut Lili Zhang verfasserin aut Wei Bai verfasserin aut Dongpo Li verfasserin aut Lijie Yang verfasserin aut Ping Gong verfasserin aut Zhanbo Wei verfasserin aut Yuchao Song verfasserin aut Lei Cui verfasserin aut Kaikuo Wu verfasserin aut Furong Xiao verfasserin aut In Forests MDPI AG, 2010 13(2022), 2, p 307 (DE-627)614095689 (DE-600)2527081-3 19994907 nnns volume:13 year:2022 number:2, p 307 https://doi.org/10.3390/f13020307 kostenfrei https://doaj.org/article/0f568bd4d0894a1dab6eef842055571f kostenfrei https://www.mdpi.com/1999-4907/13/2/307 kostenfrei https://doaj.org/toc/1999-4907 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 2, p 307
allfieldsGer	10.3390/f13020307 doi (DE-627)DOAJ014476835 (DE-599)DOAJ0f568bd4d0894a1dab6eef842055571f DE-627 ger DE-627 rakwb eng QK900-989 Yan Xue verfasserin aut Utilization of Glycine by Microorganisms along the Altitude Changbai Mountain, China: An Uptake Test Using <sup<13</sup<C,<sup<15</sup<N Labeling and <sup<13</sup<C-PLFA Analysis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier External organic nitrogen (N) inputs can contrastingly affect the transformation and availability of N in forest soils, which is an important potential N resource and is possibly vulnerable to soil properties. Little is known about the transformation and availability of external small molecule organic N in forest soils and the underlying microbial mechanisms. Soil samples from Changbai Mountain at different altitudes (from 750 m to 2200 m) that ranged widely in soil properties were incubated with <sup<13</sup<C, <sup<15</sup<N-labeled glycine. The fate of <sup<15</sup<N-glycine and the incorporation of <sup<13</sup<C into different phospholipid fatty acids (PLFAs) were measured at the same time. The addition of glycine promoted gross N mineralization and microbial N immobilization significantly. Mineralization of glycine N accounted for 6.2–22.5% of the added glycine and can be explicable in the light of a readily mineralizable substrate by soil microorganisms. Assimilation of glycine N into microbial biomass by the mineralization-immobilization-turnover (MIT) route accounted for 24.7–52.1% of the added label and was most mightily affected by the soil C/N ratio. We also found that the direct utilization of glycine is important to fulfill microorganism growth under the lack of available carbon (C) at upper elevations. The labeled glycine was rapidly incorporated into the PLFAs and was primarily assimilated by bacteria, indicating that different groups of the microbial community were answerable to external organic N. G+ bacteria were the main competitors for the exogenous glycine. Increased intact incorporation of glycine into microbial biomass and the concentration of PLFAs in general, particularly in G+ bacteria, suggest a diversified arrangement to response changes in substrate availability. glycine organic N uptake soil microorganisms PLFA Changbai Mountain Plant ecology Zhijie Wu verfasserin aut Lili Zhang verfasserin aut Wei Bai verfasserin aut Dongpo Li verfasserin aut Lijie Yang verfasserin aut Ping Gong verfasserin aut Zhanbo Wei verfasserin aut Yuchao Song verfasserin aut Lei Cui verfasserin aut Kaikuo Wu verfasserin aut Furong Xiao verfasserin aut In Forests MDPI AG, 2010 13(2022), 2, p 307 (DE-627)614095689 (DE-600)2527081-3 19994907 nnns volume:13 year:2022 number:2, p 307 https://doi.org/10.3390/f13020307 kostenfrei https://doaj.org/article/0f568bd4d0894a1dab6eef842055571f kostenfrei https://www.mdpi.com/1999-4907/13/2/307 kostenfrei https://doaj.org/toc/1999-4907 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 2, p 307
allfieldsSound	10.3390/f13020307 doi (DE-627)DOAJ014476835 (DE-599)DOAJ0f568bd4d0894a1dab6eef842055571f DE-627 ger DE-627 rakwb eng QK900-989 Yan Xue verfasserin aut Utilization of Glycine by Microorganisms along the Altitude Changbai Mountain, China: An Uptake Test Using <sup<13</sup<C,<sup<15</sup<N Labeling and <sup<13</sup<C-PLFA Analysis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier External organic nitrogen (N) inputs can contrastingly affect the transformation and availability of N in forest soils, which is an important potential N resource and is possibly vulnerable to soil properties. Little is known about the transformation and availability of external small molecule organic N in forest soils and the underlying microbial mechanisms. Soil samples from Changbai Mountain at different altitudes (from 750 m to 2200 m) that ranged widely in soil properties were incubated with <sup<13</sup<C, <sup<15</sup<N-labeled glycine. The fate of <sup<15</sup<N-glycine and the incorporation of <sup<13</sup<C into different phospholipid fatty acids (PLFAs) were measured at the same time. The addition of glycine promoted gross N mineralization and microbial N immobilization significantly. Mineralization of glycine N accounted for 6.2–22.5% of the added glycine and can be explicable in the light of a readily mineralizable substrate by soil microorganisms. Assimilation of glycine N into microbial biomass by the mineralization-immobilization-turnover (MIT) route accounted for 24.7–52.1% of the added label and was most mightily affected by the soil C/N ratio. We also found that the direct utilization of glycine is important to fulfill microorganism growth under the lack of available carbon (C) at upper elevations. The labeled glycine was rapidly incorporated into the PLFAs and was primarily assimilated by bacteria, indicating that different groups of the microbial community were answerable to external organic N. G+ bacteria were the main competitors for the exogenous glycine. Increased intact incorporation of glycine into microbial biomass and the concentration of PLFAs in general, particularly in G+ bacteria, suggest a diversified arrangement to response changes in substrate availability. glycine organic N uptake soil microorganisms PLFA Changbai Mountain Plant ecology Zhijie Wu verfasserin aut Lili Zhang verfasserin aut Wei Bai verfasserin aut Dongpo Li verfasserin aut Lijie Yang verfasserin aut Ping Gong verfasserin aut Zhanbo Wei verfasserin aut Yuchao Song verfasserin aut Lei Cui verfasserin aut Kaikuo Wu verfasserin aut Furong Xiao verfasserin aut In Forests MDPI AG, 2010 13(2022), 2, p 307 (DE-627)614095689 (DE-600)2527081-3 19994907 nnns volume:13 year:2022 number:2, p 307 https://doi.org/10.3390/f13020307 kostenfrei https://doaj.org/article/0f568bd4d0894a1dab6eef842055571f kostenfrei https://www.mdpi.com/1999-4907/13/2/307 kostenfrei https://doaj.org/toc/1999-4907 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 2, p 307
language	English
source	In Forests 13(2022), 2, p 307 volume:13 year:2022 number:2, p 307
sourceStr	In Forests 13(2022), 2, p 307 volume:13 year:2022 number:2, p 307
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	glycine organic N uptake soil microorganisms PLFA Changbai Mountain Plant ecology
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container_title	Forests
authorswithroles_txt_mv	Yan Xue @@aut@@ Zhijie Wu @@aut@@ Lili Zhang @@aut@@ Wei Bai @@aut@@ Dongpo Li @@aut@@ Lijie Yang @@aut@@ Ping Gong @@aut@@ Zhanbo Wei @@aut@@ Yuchao Song @@aut@@ Lei Cui @@aut@@ Kaikuo Wu @@aut@@ Furong Xiao @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
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callnumber-first	Q - Science
author	Yan Xue
spellingShingle	Yan Xue misc QK900-989 misc glycine misc organic N uptake misc soil microorganisms misc PLFA misc Changbai Mountain misc Plant ecology Utilization of Glycine by Microorganisms along the Altitude Changbai Mountain, China: An Uptake Test Using <sup<13</sup<C,<sup<15</sup<N Labeling and <sup<13</sup<C-PLFA Analysis
authorStr	Yan Xue
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illustrated	Not Illustrated
issn	19994907
topic_title	QK900-989 Utilization of Glycine by Microorganisms along the Altitude Changbai Mountain, China: An Uptake Test Using <sup<13</sup<C,<sup<15</sup<N Labeling and <sup<13</sup<C-PLFA Analysis glycine organic N uptake soil microorganisms PLFA Changbai Mountain
topic	misc QK900-989 misc glycine misc organic N uptake misc soil microorganisms misc PLFA misc Changbai Mountain misc Plant ecology
topic_unstemmed	misc QK900-989 misc glycine misc organic N uptake misc soil microorganisms misc PLFA misc Changbai Mountain misc Plant ecology
topic_browse	misc QK900-989 misc glycine misc organic N uptake misc soil microorganisms misc PLFA misc Changbai Mountain misc Plant ecology
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title	Utilization of Glycine by Microorganisms along the Altitude Changbai Mountain, China: An Uptake Test Using <sup<13</sup<C,<sup<15</sup<N Labeling and <sup<13</sup<C-PLFA Analysis
ctrlnum	(DE-627)DOAJ014476835 (DE-599)DOAJ0f568bd4d0894a1dab6eef842055571f
title_full	Utilization of Glycine by Microorganisms along the Altitude Changbai Mountain, China: An Uptake Test Using <sup<13</sup<C,<sup<15</sup<N Labeling and <sup<13</sup<C-PLFA Analysis
author_sort	Yan Xue
journal	Forests
journalStr	Forests
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author_browse	Yan Xue Zhijie Wu Lili Zhang Wei Bai Dongpo Li Lijie Yang Ping Gong Zhanbo Wei Yuchao Song Lei Cui Kaikuo Wu Furong Xiao
container_volume	13
class	QK900-989
format_se	Elektronische Aufsätze
author-letter	Yan Xue
doi_str_mv	10.3390/f13020307
author2-role	verfasserin
title_sort	utilization of glycine by microorganisms along the altitude changbai mountain, china: an uptake test using <sup<13</sup<c,<sup<15</sup<n labeling and <sup<13</sup<c-plfa analysis
callnumber	QK900-989
title_auth	Utilization of Glycine by Microorganisms along the Altitude Changbai Mountain, China: An Uptake Test Using <sup<13</sup<C,<sup<15</sup<N Labeling and <sup<13</sup<C-PLFA Analysis
abstract	External organic nitrogen (N) inputs can contrastingly affect the transformation and availability of N in forest soils, which is an important potential N resource and is possibly vulnerable to soil properties. Little is known about the transformation and availability of external small molecule organic N in forest soils and the underlying microbial mechanisms. Soil samples from Changbai Mountain at different altitudes (from 750 m to 2200 m) that ranged widely in soil properties were incubated with <sup<13</sup<C, <sup<15</sup<N-labeled glycine. The fate of <sup<15</sup<N-glycine and the incorporation of <sup<13</sup<C into different phospholipid fatty acids (PLFAs) were measured at the same time. The addition of glycine promoted gross N mineralization and microbial N immobilization significantly. Mineralization of glycine N accounted for 6.2–22.5% of the added glycine and can be explicable in the light of a readily mineralizable substrate by soil microorganisms. Assimilation of glycine N into microbial biomass by the mineralization-immobilization-turnover (MIT) route accounted for 24.7–52.1% of the added label and was most mightily affected by the soil C/N ratio. We also found that the direct utilization of glycine is important to fulfill microorganism growth under the lack of available carbon (C) at upper elevations. The labeled glycine was rapidly incorporated into the PLFAs and was primarily assimilated by bacteria, indicating that different groups of the microbial community were answerable to external organic N. G+ bacteria were the main competitors for the exogenous glycine. Increased intact incorporation of glycine into microbial biomass and the concentration of PLFAs in general, particularly in G+ bacteria, suggest a diversified arrangement to response changes in substrate availability.
abstractGer	External organic nitrogen (N) inputs can contrastingly affect the transformation and availability of N in forest soils, which is an important potential N resource and is possibly vulnerable to soil properties. Little is known about the transformation and availability of external small molecule organic N in forest soils and the underlying microbial mechanisms. Soil samples from Changbai Mountain at different altitudes (from 750 m to 2200 m) that ranged widely in soil properties were incubated with <sup<13</sup<C, <sup<15</sup<N-labeled glycine. The fate of <sup<15</sup<N-glycine and the incorporation of <sup<13</sup<C into different phospholipid fatty acids (PLFAs) were measured at the same time. The addition of glycine promoted gross N mineralization and microbial N immobilization significantly. Mineralization of glycine N accounted for 6.2–22.5% of the added glycine and can be explicable in the light of a readily mineralizable substrate by soil microorganisms. Assimilation of glycine N into microbial biomass by the mineralization-immobilization-turnover (MIT) route accounted for 24.7–52.1% of the added label and was most mightily affected by the soil C/N ratio. We also found that the direct utilization of glycine is important to fulfill microorganism growth under the lack of available carbon (C) at upper elevations. The labeled glycine was rapidly incorporated into the PLFAs and was primarily assimilated by bacteria, indicating that different groups of the microbial community were answerable to external organic N. G+ bacteria were the main competitors for the exogenous glycine. Increased intact incorporation of glycine into microbial biomass and the concentration of PLFAs in general, particularly in G+ bacteria, suggest a diversified arrangement to response changes in substrate availability.
abstract_unstemmed	External organic nitrogen (N) inputs can contrastingly affect the transformation and availability of N in forest soils, which is an important potential N resource and is possibly vulnerable to soil properties. Little is known about the transformation and availability of external small molecule organic N in forest soils and the underlying microbial mechanisms. Soil samples from Changbai Mountain at different altitudes (from 750 m to 2200 m) that ranged widely in soil properties were incubated with <sup<13</sup<C, <sup<15</sup<N-labeled glycine. The fate of <sup<15</sup<N-glycine and the incorporation of <sup<13</sup<C into different phospholipid fatty acids (PLFAs) were measured at the same time. The addition of glycine promoted gross N mineralization and microbial N immobilization significantly. Mineralization of glycine N accounted for 6.2–22.5% of the added glycine and can be explicable in the light of a readily mineralizable substrate by soil microorganisms. Assimilation of glycine N into microbial biomass by the mineralization-immobilization-turnover (MIT) route accounted for 24.7–52.1% of the added label and was most mightily affected by the soil C/N ratio. We also found that the direct utilization of glycine is important to fulfill microorganism growth under the lack of available carbon (C) at upper elevations. The labeled glycine was rapidly incorporated into the PLFAs and was primarily assimilated by bacteria, indicating that different groups of the microbial community were answerable to external organic N. G+ bacteria were the main competitors for the exogenous glycine. Increased intact incorporation of glycine into microbial biomass and the concentration of PLFAs in general, particularly in G+ bacteria, suggest a diversified arrangement to response changes in substrate availability.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700
container_issue	2, p 307
title_short	Utilization of Glycine by Microorganisms along the Altitude Changbai Mountain, China: An Uptake Test Using <sup<13</sup<C,<sup<15</sup<N Labeling and <sup<13</sup<C-PLFA Analysis
url	https://doi.org/10.3390/f13020307 https://doaj.org/article/0f568bd4d0894a1dab6eef842055571f https://www.mdpi.com/1999-4907/13/2/307 https://doaj.org/toc/1999-4907
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author2	Zhijie Wu Lili Zhang Wei Bai Dongpo Li Lijie Yang Ping Gong Zhanbo Wei Yuchao Song Lei Cui Kaikuo Wu Furong Xiao
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up_date	2024-07-03T23:12:20.210Z
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Utilization of Glycine by Microorganisms along the Altitude Changbai Mountain, China: An Uptake Test Using <sup<13</sup<C,<sup<15</sup<N Labeling and <sup<13</sup<C-PLFA Analysis

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