Molecular Fingerprinting and Occurrence of blaKPC and blaOXA-48 in Klebsiella pneumoniae Isolated From Clinical Specimens, Khorramabad, Iran
Objectives: Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) is created by various mechanisms mainly including the production of Klebsiella pneumoniae carbapenemase (KPC) and other class A, B, and D beta-lactamases (e.g., OXA-48). The present study was undertaken to assess the occurrence of blaKPC...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Gholamreza Goudarzi [verfasserIn] Pegah Shakib [verfasserIn] Khatereh Anbari [verfasserIn] Shahram Shokri [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2020 |
|---|
| Schlagwörter: |
|---|
| Übergeordnetes Werk: |
In: Crescent Journal of Medical and Biological Sciences - Aras Part Medical International Press, 2015, 7(2020), 3, Seite 348-354 |
|---|---|
| Übergeordnetes Werk: |
volume:7 ; year:2020 ; number:3 ; pages:348-354 |
| Links: |
|---|
| Katalog-ID: |
DOAJ01475407X |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | DOAJ01475407X | ||
| 003 | DE-627 | ||
| 005 | 20230310070849.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230226s2020 xx |||||o 00| ||eng c | ||
| 035 | |a (DE-627)DOAJ01475407X | ||
| 035 | |a (DE-599)DOAJ323e936a05b04be7bec34cb7d6dd3fe4 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 0 | |a Gholamreza Goudarzi |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Molecular Fingerprinting and Occurrence of blaKPC and blaOXA-48 in Klebsiella pneumoniae Isolated From Clinical Specimens, Khorramabad, Iran |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Objectives: Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) is created by various mechanisms mainly including the production of Klebsiella pneumoniae carbapenemase (KPC) and other class A, B, and D beta-lactamases (e.g., OXA-48). The present study was undertaken to assess the occurrence of blaKPC and blaOXA-48 genes among K. pneumoniae isolates. Materials and Methods: In this cross-sectional study, 110 K. pneumoniae strains were collected from clinical samples from 2 teaching hospitals in Khorramabad, Iran. Susceptibility testing and modified-Hodge test (MHT) for K. pneumoniae isolates were performed according to Clinical and Laboratory Standards Institute procedures, followed by carrying out the detection of blaKPC and blaOXA-48 genes by polymerase chain reaction (PCR). Finally, genetic similarity among the tested strains tested was determined by the repetitive extragenic palindromic PCR (rep-PCR) method. Results: Up to 110 K. pneumoniae, the highest resistance and susceptibility rates pertained to ampicillin and ertapenem, respectively. In addition, 10 (9%) isolates were considered as carbapenemase-producing K. pneumoniae by the MHT. Although 13 (11.8%) isolates were blaOXA-48-positive, none of them were harboring blaKPC. Eventually, 13 and 44 clusters were distinguished in hospitals A and B by rep-PCR, respectively. Conclusions: The results of this study indicated that resistance to carbapenems among K. pneumoniae isolates were relatively common in our region. Moreover, the disseminated clones in our hospitals had mainly diverse origins. | ||
| 650 | 4 | |a carbapenemase | |
| 650 | 4 | |a klebsiella pneumoniae | |
| 650 | 4 | |a blaoxa-48 | |
| 650 | 4 | |a blakpc | |
| 653 | 0 | |a Medicine | |
| 653 | 0 | |a R | |
| 700 | 0 | |a Pegah Shakib |e verfasserin |4 aut | |
| 700 | 0 | |a Khatereh Anbari |e verfasserin |4 aut | |
| 700 | 0 | |a Shahram Shokri |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i In |t Crescent Journal of Medical and Biological Sciences |d Aras Part Medical International Press, 2015 |g 7(2020), 3, Seite 348-354 |w (DE-627)818036486 |w (DE-600)2809012-3 |x 21489696 |7 nnns |
| 773 | 1 | 8 | |g volume:7 |g year:2020 |g number:3 |g pages:348-354 |
| 856 | 4 | 0 | |u https://doaj.org/article/323e936a05b04be7bec34cb7d6dd3fe4 |z kostenfrei |
| 856 | 4 | 0 | |u http://www.cjmb.org/pdf.php?id=398 |z kostenfrei |
| 856 | 4 | 2 | |u https://doaj.org/toc/2148-9696 |y Journal toc |z kostenfrei |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_DOAJ | ||
| 912 | |a GBV_ILN_20 | ||
| 912 | |a GBV_ILN_22 | ||
| 912 | |a GBV_ILN_23 | ||
| 912 | |a GBV_ILN_24 | ||
| 912 | |a GBV_ILN_39 | ||
| 912 | |a GBV_ILN_40 | ||
| 912 | |a GBV_ILN_60 | ||
| 912 | |a GBV_ILN_62 | ||
| 912 | |a GBV_ILN_63 | ||
| 912 | |a GBV_ILN_65 | ||
| 912 | |a GBV_ILN_69 | ||
| 912 | |a GBV_ILN_70 | ||
| 912 | |a GBV_ILN_73 | ||
| 912 | |a GBV_ILN_74 | ||
| 912 | |a GBV_ILN_95 | ||
| 912 | |a GBV_ILN_105 | ||
| 912 | |a GBV_ILN_110 | ||
| 912 | |a GBV_ILN_151 | ||
| 912 | |a GBV_ILN_161 | ||
| 912 | |a GBV_ILN_170 | ||
| 912 | |a GBV_ILN_206 | ||
| 912 | |a GBV_ILN_213 | ||
| 912 | |a GBV_ILN_230 | ||
| 912 | |a GBV_ILN_285 | ||
| 912 | |a GBV_ILN_293 | ||
| 912 | |a GBV_ILN_602 | ||
| 912 | |a GBV_ILN_2005 | ||
| 912 | |a GBV_ILN_2009 | ||
| 912 | |a GBV_ILN_2014 | ||
| 912 | |a GBV_ILN_2055 | ||
| 912 | |a GBV_ILN_2111 | ||
| 912 | |a GBV_ILN_4012 | ||
| 912 | |a GBV_ILN_4037 | ||
| 912 | |a GBV_ILN_4112 | ||
| 912 | |a GBV_ILN_4125 | ||
| 912 | |a GBV_ILN_4126 | ||
| 912 | |a GBV_ILN_4249 | ||
| 912 | |a GBV_ILN_4305 | ||
| 912 | |a GBV_ILN_4306 | ||
| 912 | |a GBV_ILN_4307 | ||
| 912 | |a GBV_ILN_4313 | ||
| 912 | |a GBV_ILN_4322 | ||
| 912 | |a GBV_ILN_4323 | ||
| 912 | |a GBV_ILN_4324 | ||
| 912 | |a GBV_ILN_4325 | ||
| 912 | |a GBV_ILN_4338 | ||
| 912 | |a GBV_ILN_4367 | ||
| 912 | |a GBV_ILN_4700 | ||
| 951 | |a AR | ||
| 952 | |d 7 |j 2020 |e 3 |h 348-354 | ||
| author_variant |
g g gg p s ps k a ka s s ss |
|---|---|
| matchkey_str |
article:21489696:2020----::oeuafnepitnadcurneflkcnbax4ikeselpemnaioaefoc |
| hierarchy_sort_str |
2020 |
| publishDate |
2020 |
| allfields |
(DE-627)DOAJ01475407X (DE-599)DOAJ323e936a05b04be7bec34cb7d6dd3fe4 DE-627 ger DE-627 rakwb eng Gholamreza Goudarzi verfasserin aut Molecular Fingerprinting and Occurrence of blaKPC and blaOXA-48 in Klebsiella pneumoniae Isolated From Clinical Specimens, Khorramabad, Iran 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives: Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) is created by various mechanisms mainly including the production of Klebsiella pneumoniae carbapenemase (KPC) and other class A, B, and D beta-lactamases (e.g., OXA-48). The present study was undertaken to assess the occurrence of blaKPC and blaOXA-48 genes among K. pneumoniae isolates. Materials and Methods: In this cross-sectional study, 110 K. pneumoniae strains were collected from clinical samples from 2 teaching hospitals in Khorramabad, Iran. Susceptibility testing and modified-Hodge test (MHT) for K. pneumoniae isolates were performed according to Clinical and Laboratory Standards Institute procedures, followed by carrying out the detection of blaKPC and blaOXA-48 genes by polymerase chain reaction (PCR). Finally, genetic similarity among the tested strains tested was determined by the repetitive extragenic palindromic PCR (rep-PCR) method. Results: Up to 110 K. pneumoniae, the highest resistance and susceptibility rates pertained to ampicillin and ertapenem, respectively. In addition, 10 (9%) isolates were considered as carbapenemase-producing K. pneumoniae by the MHT. Although 13 (11.8%) isolates were blaOXA-48-positive, none of them were harboring blaKPC. Eventually, 13 and 44 clusters were distinguished in hospitals A and B by rep-PCR, respectively. Conclusions: The results of this study indicated that resistance to carbapenems among K. pneumoniae isolates were relatively common in our region. Moreover, the disseminated clones in our hospitals had mainly diverse origins. carbapenemase klebsiella pneumoniae blaoxa-48 blakpc Medicine R Pegah Shakib verfasserin aut Khatereh Anbari verfasserin aut Shahram Shokri verfasserin aut In Crescent Journal of Medical and Biological Sciences Aras Part Medical International Press, 2015 7(2020), 3, Seite 348-354 (DE-627)818036486 (DE-600)2809012-3 21489696 nnns volume:7 year:2020 number:3 pages:348-354 https://doaj.org/article/323e936a05b04be7bec34cb7d6dd3fe4 kostenfrei http://www.cjmb.org/pdf.php?id=398 kostenfrei https://doaj.org/toc/2148-9696 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2020 3 348-354 |
| spelling |
(DE-627)DOAJ01475407X (DE-599)DOAJ323e936a05b04be7bec34cb7d6dd3fe4 DE-627 ger DE-627 rakwb eng Gholamreza Goudarzi verfasserin aut Molecular Fingerprinting and Occurrence of blaKPC and blaOXA-48 in Klebsiella pneumoniae Isolated From Clinical Specimens, Khorramabad, Iran 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives: Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) is created by various mechanisms mainly including the production of Klebsiella pneumoniae carbapenemase (KPC) and other class A, B, and D beta-lactamases (e.g., OXA-48). The present study was undertaken to assess the occurrence of blaKPC and blaOXA-48 genes among K. pneumoniae isolates. Materials and Methods: In this cross-sectional study, 110 K. pneumoniae strains were collected from clinical samples from 2 teaching hospitals in Khorramabad, Iran. Susceptibility testing and modified-Hodge test (MHT) for K. pneumoniae isolates were performed according to Clinical and Laboratory Standards Institute procedures, followed by carrying out the detection of blaKPC and blaOXA-48 genes by polymerase chain reaction (PCR). Finally, genetic similarity among the tested strains tested was determined by the repetitive extragenic palindromic PCR (rep-PCR) method. Results: Up to 110 K. pneumoniae, the highest resistance and susceptibility rates pertained to ampicillin and ertapenem, respectively. In addition, 10 (9%) isolates were considered as carbapenemase-producing K. pneumoniae by the MHT. Although 13 (11.8%) isolates were blaOXA-48-positive, none of them were harboring blaKPC. Eventually, 13 and 44 clusters were distinguished in hospitals A and B by rep-PCR, respectively. Conclusions: The results of this study indicated that resistance to carbapenems among K. pneumoniae isolates were relatively common in our region. Moreover, the disseminated clones in our hospitals had mainly diverse origins. carbapenemase klebsiella pneumoniae blaoxa-48 blakpc Medicine R Pegah Shakib verfasserin aut Khatereh Anbari verfasserin aut Shahram Shokri verfasserin aut In Crescent Journal of Medical and Biological Sciences Aras Part Medical International Press, 2015 7(2020), 3, Seite 348-354 (DE-627)818036486 (DE-600)2809012-3 21489696 nnns volume:7 year:2020 number:3 pages:348-354 https://doaj.org/article/323e936a05b04be7bec34cb7d6dd3fe4 kostenfrei http://www.cjmb.org/pdf.php?id=398 kostenfrei https://doaj.org/toc/2148-9696 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2020 3 348-354 |
| allfields_unstemmed |
(DE-627)DOAJ01475407X (DE-599)DOAJ323e936a05b04be7bec34cb7d6dd3fe4 DE-627 ger DE-627 rakwb eng Gholamreza Goudarzi verfasserin aut Molecular Fingerprinting and Occurrence of blaKPC and blaOXA-48 in Klebsiella pneumoniae Isolated From Clinical Specimens, Khorramabad, Iran 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives: Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) is created by various mechanisms mainly including the production of Klebsiella pneumoniae carbapenemase (KPC) and other class A, B, and D beta-lactamases (e.g., OXA-48). The present study was undertaken to assess the occurrence of blaKPC and blaOXA-48 genes among K. pneumoniae isolates. Materials and Methods: In this cross-sectional study, 110 K. pneumoniae strains were collected from clinical samples from 2 teaching hospitals in Khorramabad, Iran. Susceptibility testing and modified-Hodge test (MHT) for K. pneumoniae isolates were performed according to Clinical and Laboratory Standards Institute procedures, followed by carrying out the detection of blaKPC and blaOXA-48 genes by polymerase chain reaction (PCR). Finally, genetic similarity among the tested strains tested was determined by the repetitive extragenic palindromic PCR (rep-PCR) method. Results: Up to 110 K. pneumoniae, the highest resistance and susceptibility rates pertained to ampicillin and ertapenem, respectively. In addition, 10 (9%) isolates were considered as carbapenemase-producing K. pneumoniae by the MHT. Although 13 (11.8%) isolates were blaOXA-48-positive, none of them were harboring blaKPC. Eventually, 13 and 44 clusters were distinguished in hospitals A and B by rep-PCR, respectively. Conclusions: The results of this study indicated that resistance to carbapenems among K. pneumoniae isolates were relatively common in our region. Moreover, the disseminated clones in our hospitals had mainly diverse origins. carbapenemase klebsiella pneumoniae blaoxa-48 blakpc Medicine R Pegah Shakib verfasserin aut Khatereh Anbari verfasserin aut Shahram Shokri verfasserin aut In Crescent Journal of Medical and Biological Sciences Aras Part Medical International Press, 2015 7(2020), 3, Seite 348-354 (DE-627)818036486 (DE-600)2809012-3 21489696 nnns volume:7 year:2020 number:3 pages:348-354 https://doaj.org/article/323e936a05b04be7bec34cb7d6dd3fe4 kostenfrei http://www.cjmb.org/pdf.php?id=398 kostenfrei https://doaj.org/toc/2148-9696 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2020 3 348-354 |
| allfieldsGer |
(DE-627)DOAJ01475407X (DE-599)DOAJ323e936a05b04be7bec34cb7d6dd3fe4 DE-627 ger DE-627 rakwb eng Gholamreza Goudarzi verfasserin aut Molecular Fingerprinting and Occurrence of blaKPC and blaOXA-48 in Klebsiella pneumoniae Isolated From Clinical Specimens, Khorramabad, Iran 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives: Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) is created by various mechanisms mainly including the production of Klebsiella pneumoniae carbapenemase (KPC) and other class A, B, and D beta-lactamases (e.g., OXA-48). The present study was undertaken to assess the occurrence of blaKPC and blaOXA-48 genes among K. pneumoniae isolates. Materials and Methods: In this cross-sectional study, 110 K. pneumoniae strains were collected from clinical samples from 2 teaching hospitals in Khorramabad, Iran. Susceptibility testing and modified-Hodge test (MHT) for K. pneumoniae isolates were performed according to Clinical and Laboratory Standards Institute procedures, followed by carrying out the detection of blaKPC and blaOXA-48 genes by polymerase chain reaction (PCR). Finally, genetic similarity among the tested strains tested was determined by the repetitive extragenic palindromic PCR (rep-PCR) method. Results: Up to 110 K. pneumoniae, the highest resistance and susceptibility rates pertained to ampicillin and ertapenem, respectively. In addition, 10 (9%) isolates were considered as carbapenemase-producing K. pneumoniae by the MHT. Although 13 (11.8%) isolates were blaOXA-48-positive, none of them were harboring blaKPC. Eventually, 13 and 44 clusters were distinguished in hospitals A and B by rep-PCR, respectively. Conclusions: The results of this study indicated that resistance to carbapenems among K. pneumoniae isolates were relatively common in our region. Moreover, the disseminated clones in our hospitals had mainly diverse origins. carbapenemase klebsiella pneumoniae blaoxa-48 blakpc Medicine R Pegah Shakib verfasserin aut Khatereh Anbari verfasserin aut Shahram Shokri verfasserin aut In Crescent Journal of Medical and Biological Sciences Aras Part Medical International Press, 2015 7(2020), 3, Seite 348-354 (DE-627)818036486 (DE-600)2809012-3 21489696 nnns volume:7 year:2020 number:3 pages:348-354 https://doaj.org/article/323e936a05b04be7bec34cb7d6dd3fe4 kostenfrei http://www.cjmb.org/pdf.php?id=398 kostenfrei https://doaj.org/toc/2148-9696 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2020 3 348-354 |
| allfieldsSound |
(DE-627)DOAJ01475407X (DE-599)DOAJ323e936a05b04be7bec34cb7d6dd3fe4 DE-627 ger DE-627 rakwb eng Gholamreza Goudarzi verfasserin aut Molecular Fingerprinting and Occurrence of blaKPC and blaOXA-48 in Klebsiella pneumoniae Isolated From Clinical Specimens, Khorramabad, Iran 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives: Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) is created by various mechanisms mainly including the production of Klebsiella pneumoniae carbapenemase (KPC) and other class A, B, and D beta-lactamases (e.g., OXA-48). The present study was undertaken to assess the occurrence of blaKPC and blaOXA-48 genes among K. pneumoniae isolates. Materials and Methods: In this cross-sectional study, 110 K. pneumoniae strains were collected from clinical samples from 2 teaching hospitals in Khorramabad, Iran. Susceptibility testing and modified-Hodge test (MHT) for K. pneumoniae isolates were performed according to Clinical and Laboratory Standards Institute procedures, followed by carrying out the detection of blaKPC and blaOXA-48 genes by polymerase chain reaction (PCR). Finally, genetic similarity among the tested strains tested was determined by the repetitive extragenic palindromic PCR (rep-PCR) method. Results: Up to 110 K. pneumoniae, the highest resistance and susceptibility rates pertained to ampicillin and ertapenem, respectively. In addition, 10 (9%) isolates were considered as carbapenemase-producing K. pneumoniae by the MHT. Although 13 (11.8%) isolates were blaOXA-48-positive, none of them were harboring blaKPC. Eventually, 13 and 44 clusters were distinguished in hospitals A and B by rep-PCR, respectively. Conclusions: The results of this study indicated that resistance to carbapenems among K. pneumoniae isolates were relatively common in our region. Moreover, the disseminated clones in our hospitals had mainly diverse origins. carbapenemase klebsiella pneumoniae blaoxa-48 blakpc Medicine R Pegah Shakib verfasserin aut Khatereh Anbari verfasserin aut Shahram Shokri verfasserin aut In Crescent Journal of Medical and Biological Sciences Aras Part Medical International Press, 2015 7(2020), 3, Seite 348-354 (DE-627)818036486 (DE-600)2809012-3 21489696 nnns volume:7 year:2020 number:3 pages:348-354 https://doaj.org/article/323e936a05b04be7bec34cb7d6dd3fe4 kostenfrei http://www.cjmb.org/pdf.php?id=398 kostenfrei https://doaj.org/toc/2148-9696 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2020 3 348-354 |
| language |
English |
| source |
In Crescent Journal of Medical and Biological Sciences 7(2020), 3, Seite 348-354 volume:7 year:2020 number:3 pages:348-354 |
| sourceStr |
In Crescent Journal of Medical and Biological Sciences 7(2020), 3, Seite 348-354 volume:7 year:2020 number:3 pages:348-354 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| topic_facet |
carbapenemase klebsiella pneumoniae blaoxa-48 blakpc Medicine R |
| isfreeaccess_bool |
true |
| container_title |
Crescent Journal of Medical and Biological Sciences |
| authorswithroles_txt_mv |
Gholamreza Goudarzi @@aut@@ Pegah Shakib @@aut@@ Khatereh Anbari @@aut@@ Shahram Shokri @@aut@@ |
| publishDateDaySort_date |
2020-01-01T00:00:00Z |
| hierarchy_top_id |
818036486 |
| id |
DOAJ01475407X |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ01475407X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230310070849.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230226s2020 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ01475407X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ323e936a05b04be7bec34cb7d6dd3fe4</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Gholamreza Goudarzi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Molecular Fingerprinting and Occurrence of blaKPC and blaOXA-48 in Klebsiella pneumoniae Isolated From Clinical Specimens, Khorramabad, Iran</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2020</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Objectives: Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) is created by various mechanisms mainly including the production of Klebsiella pneumoniae carbapenemase (KPC) and other class A, B, and D beta-lactamases (e.g., OXA-48). The present study was undertaken to assess the occurrence of blaKPC and blaOXA-48 genes among K. pneumoniae isolates. Materials and Methods: In this cross-sectional study, 110 K. pneumoniae strains were collected from clinical samples from 2 teaching hospitals in Khorramabad, Iran. Susceptibility testing and modified-Hodge test (MHT) for K. pneumoniae isolates were performed according to Clinical and Laboratory Standards Institute procedures, followed by carrying out the detection of blaKPC and blaOXA-48 genes by polymerase chain reaction (PCR). Finally, genetic similarity among the tested strains tested was determined by the repetitive extragenic palindromic PCR (rep-PCR) method. Results: Up to 110 K. pneumoniae, the highest resistance and susceptibility rates pertained to ampicillin and ertapenem, respectively. In addition, 10 (9%) isolates were considered as carbapenemase-producing K. pneumoniae by the MHT. Although 13 (11.8%) isolates were blaOXA-48-positive, none of them were harboring blaKPC. Eventually, 13 and 44 clusters were distinguished in hospitals A and B by rep-PCR, respectively. Conclusions: The results of this study indicated that resistance to carbapenems among K. pneumoniae isolates were relatively common in our region. Moreover, the disseminated clones in our hospitals had mainly diverse origins.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">carbapenemase</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">klebsiella pneumoniae</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">blaoxa-48</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">blakpc</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Medicine</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">R</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Pegah Shakib</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Khatereh Anbari</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Shahram Shokri</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Crescent Journal of Medical and Biological Sciences</subfield><subfield code="d">Aras Part Medical International Press, 2015</subfield><subfield code="g">7(2020), 3, Seite 348-354</subfield><subfield code="w">(DE-627)818036486</subfield><subfield code="w">(DE-600)2809012-3</subfield><subfield code="x">21489696</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:7</subfield><subfield code="g">year:2020</subfield><subfield code="g">number:3</subfield><subfield code="g">pages:348-354</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/323e936a05b04be7bec34cb7d6dd3fe4</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://www.cjmb.org/pdf.php?id=398</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2148-9696</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">7</subfield><subfield code="j">2020</subfield><subfield code="e">3</subfield><subfield code="h">348-354</subfield></datafield></record></collection>
|
| author |
Gholamreza Goudarzi |
| spellingShingle |
Gholamreza Goudarzi misc carbapenemase misc klebsiella pneumoniae misc blaoxa-48 misc blakpc misc Medicine misc R Molecular Fingerprinting and Occurrence of blaKPC and blaOXA-48 in Klebsiella pneumoniae Isolated From Clinical Specimens, Khorramabad, Iran |
| authorStr |
Gholamreza Goudarzi |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)818036486 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| author_role |
aut aut aut aut |
| collection |
DOAJ |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
21489696 |
| topic_title |
Molecular Fingerprinting and Occurrence of blaKPC and blaOXA-48 in Klebsiella pneumoniae Isolated From Clinical Specimens, Khorramabad, Iran carbapenemase klebsiella pneumoniae blaoxa-48 blakpc |
| topic |
misc carbapenemase misc klebsiella pneumoniae misc blaoxa-48 misc blakpc misc Medicine misc R |
| topic_unstemmed |
misc carbapenemase misc klebsiella pneumoniae misc blaoxa-48 misc blakpc misc Medicine misc R |
| topic_browse |
misc carbapenemase misc klebsiella pneumoniae misc blaoxa-48 misc blakpc misc Medicine misc R |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
cr |
| hierarchy_parent_title |
Crescent Journal of Medical and Biological Sciences |
| hierarchy_parent_id |
818036486 |
| hierarchy_top_title |
Crescent Journal of Medical and Biological Sciences |
| isfreeaccess_txt |
true |
| familylinks_str_mv |
(DE-627)818036486 (DE-600)2809012-3 |
| title |
Molecular Fingerprinting and Occurrence of blaKPC and blaOXA-48 in Klebsiella pneumoniae Isolated From Clinical Specimens, Khorramabad, Iran |
| ctrlnum |
(DE-627)DOAJ01475407X (DE-599)DOAJ323e936a05b04be7bec34cb7d6dd3fe4 |
| title_full |
Molecular Fingerprinting and Occurrence of blaKPC and blaOXA-48 in Klebsiella pneumoniae Isolated From Clinical Specimens, Khorramabad, Iran |
| author_sort |
Gholamreza Goudarzi |
| journal |
Crescent Journal of Medical and Biological Sciences |
| journalStr |
Crescent Journal of Medical and Biological Sciences |
| lang_code |
eng |
| isOA_bool |
true |
| recordtype |
marc |
| publishDateSort |
2020 |
| contenttype_str_mv |
txt |
| container_start_page |
348 |
| author_browse |
Gholamreza Goudarzi Pegah Shakib Khatereh Anbari Shahram Shokri |
| container_volume |
7 |
| format_se |
Elektronische Aufsätze |
| author-letter |
Gholamreza Goudarzi |
| author2-role |
verfasserin |
| title_sort |
molecular fingerprinting and occurrence of blakpc and blaoxa-48 in klebsiella pneumoniae isolated from clinical specimens, khorramabad, iran |
| title_auth |
Molecular Fingerprinting and Occurrence of blaKPC and blaOXA-48 in Klebsiella pneumoniae Isolated From Clinical Specimens, Khorramabad, Iran |
| abstract |
Objectives: Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) is created by various mechanisms mainly including the production of Klebsiella pneumoniae carbapenemase (KPC) and other class A, B, and D beta-lactamases (e.g., OXA-48). The present study was undertaken to assess the occurrence of blaKPC and blaOXA-48 genes among K. pneumoniae isolates. Materials and Methods: In this cross-sectional study, 110 K. pneumoniae strains were collected from clinical samples from 2 teaching hospitals in Khorramabad, Iran. Susceptibility testing and modified-Hodge test (MHT) for K. pneumoniae isolates were performed according to Clinical and Laboratory Standards Institute procedures, followed by carrying out the detection of blaKPC and blaOXA-48 genes by polymerase chain reaction (PCR). Finally, genetic similarity among the tested strains tested was determined by the repetitive extragenic palindromic PCR (rep-PCR) method. Results: Up to 110 K. pneumoniae, the highest resistance and susceptibility rates pertained to ampicillin and ertapenem, respectively. In addition, 10 (9%) isolates were considered as carbapenemase-producing K. pneumoniae by the MHT. Although 13 (11.8%) isolates were blaOXA-48-positive, none of them were harboring blaKPC. Eventually, 13 and 44 clusters were distinguished in hospitals A and B by rep-PCR, respectively. Conclusions: The results of this study indicated that resistance to carbapenems among K. pneumoniae isolates were relatively common in our region. Moreover, the disseminated clones in our hospitals had mainly diverse origins. |
| abstractGer |
Objectives: Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) is created by various mechanisms mainly including the production of Klebsiella pneumoniae carbapenemase (KPC) and other class A, B, and D beta-lactamases (e.g., OXA-48). The present study was undertaken to assess the occurrence of blaKPC and blaOXA-48 genes among K. pneumoniae isolates. Materials and Methods: In this cross-sectional study, 110 K. pneumoniae strains were collected from clinical samples from 2 teaching hospitals in Khorramabad, Iran. Susceptibility testing and modified-Hodge test (MHT) for K. pneumoniae isolates were performed according to Clinical and Laboratory Standards Institute procedures, followed by carrying out the detection of blaKPC and blaOXA-48 genes by polymerase chain reaction (PCR). Finally, genetic similarity among the tested strains tested was determined by the repetitive extragenic palindromic PCR (rep-PCR) method. Results: Up to 110 K. pneumoniae, the highest resistance and susceptibility rates pertained to ampicillin and ertapenem, respectively. In addition, 10 (9%) isolates were considered as carbapenemase-producing K. pneumoniae by the MHT. Although 13 (11.8%) isolates were blaOXA-48-positive, none of them were harboring blaKPC. Eventually, 13 and 44 clusters were distinguished in hospitals A and B by rep-PCR, respectively. Conclusions: The results of this study indicated that resistance to carbapenems among K. pneumoniae isolates were relatively common in our region. Moreover, the disseminated clones in our hospitals had mainly diverse origins. |
| abstract_unstemmed |
Objectives: Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) is created by various mechanisms mainly including the production of Klebsiella pneumoniae carbapenemase (KPC) and other class A, B, and D beta-lactamases (e.g., OXA-48). The present study was undertaken to assess the occurrence of blaKPC and blaOXA-48 genes among K. pneumoniae isolates. Materials and Methods: In this cross-sectional study, 110 K. pneumoniae strains were collected from clinical samples from 2 teaching hospitals in Khorramabad, Iran. Susceptibility testing and modified-Hodge test (MHT) for K. pneumoniae isolates were performed according to Clinical and Laboratory Standards Institute procedures, followed by carrying out the detection of blaKPC and blaOXA-48 genes by polymerase chain reaction (PCR). Finally, genetic similarity among the tested strains tested was determined by the repetitive extragenic palindromic PCR (rep-PCR) method. Results: Up to 110 K. pneumoniae, the highest resistance and susceptibility rates pertained to ampicillin and ertapenem, respectively. In addition, 10 (9%) isolates were considered as carbapenemase-producing K. pneumoniae by the MHT. Although 13 (11.8%) isolates were blaOXA-48-positive, none of them were harboring blaKPC. Eventually, 13 and 44 clusters were distinguished in hospitals A and B by rep-PCR, respectively. Conclusions: The results of this study indicated that resistance to carbapenems among K. pneumoniae isolates were relatively common in our region. Moreover, the disseminated clones in our hospitals had mainly diverse origins. |
| collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 |
| container_issue |
3 |
| title_short |
Molecular Fingerprinting and Occurrence of blaKPC and blaOXA-48 in Klebsiella pneumoniae Isolated From Clinical Specimens, Khorramabad, Iran |
| url |
https://doaj.org/article/323e936a05b04be7bec34cb7d6dd3fe4 http://www.cjmb.org/pdf.php?id=398 https://doaj.org/toc/2148-9696 |
| remote_bool |
true |
| author2 |
Pegah Shakib Khatereh Anbari Shahram Shokri |
| author2Str |
Pegah Shakib Khatereh Anbari Shahram Shokri |
| ppnlink |
818036486 |
| mediatype_str_mv |
c |
| isOA_txt |
true |
| hochschulschrift_bool |
false |
| up_date |
2024-07-04T00:21:24.556Z |
| _version_ |
1803605754049986560 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ01475407X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230310070849.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230226s2020 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ01475407X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ323e936a05b04be7bec34cb7d6dd3fe4</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Gholamreza Goudarzi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Molecular Fingerprinting and Occurrence of blaKPC and blaOXA-48 in Klebsiella pneumoniae Isolated From Clinical Specimens, Khorramabad, Iran</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2020</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Objectives: Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) is created by various mechanisms mainly including the production of Klebsiella pneumoniae carbapenemase (KPC) and other class A, B, and D beta-lactamases (e.g., OXA-48). The present study was undertaken to assess the occurrence of blaKPC and blaOXA-48 genes among K. pneumoniae isolates. Materials and Methods: In this cross-sectional study, 110 K. pneumoniae strains were collected from clinical samples from 2 teaching hospitals in Khorramabad, Iran. Susceptibility testing and modified-Hodge test (MHT) for K. pneumoniae isolates were performed according to Clinical and Laboratory Standards Institute procedures, followed by carrying out the detection of blaKPC and blaOXA-48 genes by polymerase chain reaction (PCR). Finally, genetic similarity among the tested strains tested was determined by the repetitive extragenic palindromic PCR (rep-PCR) method. Results: Up to 110 K. pneumoniae, the highest resistance and susceptibility rates pertained to ampicillin and ertapenem, respectively. In addition, 10 (9%) isolates were considered as carbapenemase-producing K. pneumoniae by the MHT. Although 13 (11.8%) isolates were blaOXA-48-positive, none of them were harboring blaKPC. Eventually, 13 and 44 clusters were distinguished in hospitals A and B by rep-PCR, respectively. Conclusions: The results of this study indicated that resistance to carbapenems among K. pneumoniae isolates were relatively common in our region. Moreover, the disseminated clones in our hospitals had mainly diverse origins.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">carbapenemase</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">klebsiella pneumoniae</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">blaoxa-48</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">blakpc</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Medicine</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">R</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Pegah Shakib</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Khatereh Anbari</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Shahram Shokri</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Crescent Journal of Medical and Biological Sciences</subfield><subfield code="d">Aras Part Medical International Press, 2015</subfield><subfield code="g">7(2020), 3, Seite 348-354</subfield><subfield code="w">(DE-627)818036486</subfield><subfield code="w">(DE-600)2809012-3</subfield><subfield code="x">21489696</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:7</subfield><subfield code="g">year:2020</subfield><subfield code="g">number:3</subfield><subfield code="g">pages:348-354</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/323e936a05b04be7bec34cb7d6dd3fe4</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://www.cjmb.org/pdf.php?id=398</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2148-9696</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">7</subfield><subfield code="j">2020</subfield><subfield code="e">3</subfield><subfield code="h">348-354</subfield></datafield></record></collection>
|
| score |
7.3986626 |