Surface Structuring Combined with Chemical Surface Functionalization: An Effective Tool to Manipulate Cell Adhesion

In this study, we investigate how a surface structure underneath a surface-attached polymer coating affects the bioactivity of the resulting material. To that end, structured surfaces were fabricated using colloidal lithography (lateral dimensions: 200 nm to 1 µm, height ~15 to 50 nm). The...
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Gespeichert in:

Autor*in:	Sarah M. Elsayed [verfasserIn] Stefan Paschke [verfasserIn] Sibylle J. Rau [verfasserIn] Karen Lienkamp [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	colloidal lithography polymer surfaces surface functionalization surface-cell interactions surface structuring tissue engineering

Übergeordnetes Werk:	In: Molecules - MDPI AG, 2003, 24(2019), 5, p 909
Übergeordnetes Werk:	volume:24 ; year:2019 ; number:5, p 909

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/molecules24050909

Katalog-ID:	DOAJ014841800

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language	English
source	In Molecules 24(2019), 5, p 909 volume:24 year:2019 number:5, p 909
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institution	findex.gbv.de
topic_facet	colloidal lithography polymer surfaces surface functionalization surface-cell interactions surface structuring tissue engineering Organic chemistry
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authorswithroles_txt_mv	Sarah M. Elsayed @@aut@@ Stefan Paschke @@aut@@ Sibylle J. Rau @@aut@@ Karen Lienkamp @@aut@@
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callnumber-first	Q - Science
author	Sarah M. Elsayed
spellingShingle	Sarah M. Elsayed misc QD241-441 misc colloidal lithography misc polymer surfaces misc surface functionalization misc surface-cell interactions misc surface structuring misc tissue engineering misc Organic chemistry Surface Structuring Combined with Chemical Surface Functionalization: An Effective Tool to Manipulate Cell Adhesion
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topic_title	QD241-441 Surface Structuring Combined with Chemical Surface Functionalization: An Effective Tool to Manipulate Cell Adhesion colloidal lithography polymer surfaces surface functionalization surface-cell interactions surface structuring tissue engineering
topic	misc QD241-441 misc colloidal lithography misc polymer surfaces misc surface functionalization misc surface-cell interactions misc surface structuring misc tissue engineering misc Organic chemistry
topic_unstemmed	misc QD241-441 misc colloidal lithography misc polymer surfaces misc surface functionalization misc surface-cell interactions misc surface structuring misc tissue engineering misc Organic chemistry
topic_browse	misc QD241-441 misc colloidal lithography misc polymer surfaces misc surface functionalization misc surface-cell interactions misc surface structuring misc tissue engineering misc Organic chemistry
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title	Surface Structuring Combined with Chemical Surface Functionalization: An Effective Tool to Manipulate Cell Adhesion
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author_browse	Sarah M. Elsayed Stefan Paschke Sibylle J. Rau Karen Lienkamp
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title_sort	surface structuring combined with chemical surface functionalization: an effective tool to manipulate cell adhesion
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title_auth	Surface Structuring Combined with Chemical Surface Functionalization: An Effective Tool to Manipulate Cell Adhesion
abstract	In this study, we investigate how a surface structure underneath a surface-attached polymer coating affects the bioactivity of the resulting material. To that end, structured surfaces were fabricated using colloidal lithography (lateral dimensions: 200 nm to 1 µm, height ~15 to 50 nm). The surface structures were further functionalized either with antimicrobial, cell-adhesive polycations or with protein-repellent polyzwitterions. The materials thus obtained were compared to non-functionalized structured surfaces and unstructured polymer monolayers. Their physical properties were studied by contact-angle measurements and atomic force microscopy (AFM). Protein adhesion was studied by surface plasmon resonance spectroscopy, and the antimicrobial activity against Escherichia coli bacteria was tested. The growth of human mucosal gingiva keratinocytes on the materials was analyzed using the Alamar blue assay, optical microscopy, and live-dead staining. The data shows that the underlying surface structure itself reduced protein adhesion and also bacterial adhesion, as evidenced by increased antimicrobial activity. It also enhanced cell adhesion to the surfaces. Particularly in combination with the adhesive polycations, the surfaces increased the cell growth compared to the unstructured reference materials. Thus, functionalizing structured surfaces with adhesive polymer could be a valuable tool for improved tissue integration.
abstractGer	In this study, we investigate how a surface structure underneath a surface-attached polymer coating affects the bioactivity of the resulting material. To that end, structured surfaces were fabricated using colloidal lithography (lateral dimensions: 200 nm to 1 µm, height ~15 to 50 nm). The surface structures were further functionalized either with antimicrobial, cell-adhesive polycations or with protein-repellent polyzwitterions. The materials thus obtained were compared to non-functionalized structured surfaces and unstructured polymer monolayers. Their physical properties were studied by contact-angle measurements and atomic force microscopy (AFM). Protein adhesion was studied by surface plasmon resonance spectroscopy, and the antimicrobial activity against Escherichia coli bacteria was tested. The growth of human mucosal gingiva keratinocytes on the materials was analyzed using the Alamar blue assay, optical microscopy, and live-dead staining. The data shows that the underlying surface structure itself reduced protein adhesion and also bacterial adhesion, as evidenced by increased antimicrobial activity. It also enhanced cell adhesion to the surfaces. Particularly in combination with the adhesive polycations, the surfaces increased the cell growth compared to the unstructured reference materials. Thus, functionalizing structured surfaces with adhesive polymer could be a valuable tool for improved tissue integration.
abstract_unstemmed	In this study, we investigate how a surface structure underneath a surface-attached polymer coating affects the bioactivity of the resulting material. To that end, structured surfaces were fabricated using colloidal lithography (lateral dimensions: 200 nm to 1 µm, height ~15 to 50 nm). The surface structures were further functionalized either with antimicrobial, cell-adhesive polycations or with protein-repellent polyzwitterions. The materials thus obtained were compared to non-functionalized structured surfaces and unstructured polymer monolayers. Their physical properties were studied by contact-angle measurements and atomic force microscopy (AFM). Protein adhesion was studied by surface plasmon resonance spectroscopy, and the antimicrobial activity against Escherichia coli bacteria was tested. The growth of human mucosal gingiva keratinocytes on the materials was analyzed using the Alamar blue assay, optical microscopy, and live-dead staining. The data shows that the underlying surface structure itself reduced protein adhesion and also bacterial adhesion, as evidenced by increased antimicrobial activity. It also enhanced cell adhesion to the surfaces. Particularly in combination with the adhesive polycations, the surfaces increased the cell growth compared to the unstructured reference materials. Thus, functionalizing structured surfaces with adhesive polymer could be a valuable tool for improved tissue integration.
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title_short	Surface Structuring Combined with Chemical Surface Functionalization: An Effective Tool to Manipulate Cell Adhesion
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