Extracorporeal Membrane Oxygenation-Induced Hemolysis: An In Vitro Study to Appraise Causative Factors

In vitro hemolysis testing is commonly used to determine hemocompatibility of ExtraCorporeal Membrane Oxygenation (ECMO). However, poor reproducibility remains a challenging problem, due to several unidentified influencing factors. The present study investigated potential factors, such as flow rates...
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Autor*in:	Chris Hoi Houng Chan [verfasserIn] Katrina K. Ki [verfasserIn] Meili Zhang [verfasserIn] Cooper Asnicar [verfasserIn] Hwa Jin Cho [verfasserIn] Carmen Ainola [verfasserIn] Mahe Bouquet [verfasserIn] Silver Heinsar [verfasserIn] Jo Philipp Pauls [verfasserIn] Gianluigi Li Bassi [verfasserIn] Jacky Suen [verfasserIn] John F. Fraser [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	mechanical circulatory device blood trauma anticoagulants blood donor gender flow rate in vitro model

Übergeordnetes Werk:	In: Membranes - MDPI AG, 2011, 11(2021), 5, p 313
Übergeordnetes Werk:	volume:11 ; year:2021 ; number:5, p 313

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/membranes11050313

Katalog-ID:	DOAJ015079651

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allfields_unstemmed	10.3390/membranes11050313 doi (DE-627)DOAJ015079651 (DE-599)DOAJ84053e8fbf7b463ba90550a2d76d5cbf DE-627 ger DE-627 rakwb eng TP1-1185 TP155-156 Chris Hoi Houng Chan verfasserin aut Extracorporeal Membrane Oxygenation-Induced Hemolysis: An In Vitro Study to Appraise Causative Factors 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In vitro hemolysis testing is commonly used to determine hemocompatibility of ExtraCorporeal Membrane Oxygenation (ECMO). However, poor reproducibility remains a challenging problem, due to several unidentified influencing factors. The present study investigated potential factors, such as flow rates, the use of anticoagulants, and gender of blood donors, which could play a role in hemolysis. Fresh human whole blood was anticoagulated with either citrate (n = 6) or heparin (n = 12; 6 female and 6 male blood donors). Blood was then circulated for 360 min at 4 L/min or 1.5 L/min. Regardless of flow rate conditions, hemolysis remained unchanged over time in citrated blood, but significantly increased after 240 min circulation in heparinized blood (<i<p</i< ≤ 0.01). The ratio of the normalized index of hemolysis (<i<NIH</i<) of heparinized blood to citrated blood was 11.7-fold higher at 4 L/min and 16.5–fold higher at 1.5 L/min. The difference in hemolysis between 1.5 L/min and 4 L/min concurred with findings of previous literature. In addition, the ratio of <i<NIH</i< of male heparinized blood to female was 1.7-fold higher at 4 L/min and 2.2-fold higher at 1.5 L/min. Our preliminary results suggested that the choice of anticoagulant and blood donor gender could be critical factors in hemolysis studies, and should be taken into account to improve testing reliability during ECMO. mechanical circulatory device blood trauma anticoagulants blood donor gender flow rate in vitro model Chemical technology Chemical engineering Katrina K. Ki verfasserin aut Meili Zhang verfasserin aut Cooper Asnicar verfasserin aut Hwa Jin Cho verfasserin aut Carmen Ainola verfasserin aut Mahe Bouquet verfasserin aut Silver Heinsar verfasserin aut Jo Philipp Pauls verfasserin aut Gianluigi Li Bassi verfasserin aut Jacky Suen verfasserin aut John F. Fraser verfasserin aut In Membranes MDPI AG, 2011 11(2021), 5, p 313 (DE-627)662495683 (DE-600)2614641-1 20770375 nnns volume:11 year:2021 number:5, p 313 https://doi.org/10.3390/membranes11050313 kostenfrei https://doaj.org/article/84053e8fbf7b463ba90550a2d76d5cbf kostenfrei https://www.mdpi.com/2077-0375/11/5/313 kostenfrei https://doaj.org/toc/2077-0375 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2021 5, p 313
allfieldsGer	10.3390/membranes11050313 doi (DE-627)DOAJ015079651 (DE-599)DOAJ84053e8fbf7b463ba90550a2d76d5cbf DE-627 ger DE-627 rakwb eng TP1-1185 TP155-156 Chris Hoi Houng Chan verfasserin aut Extracorporeal Membrane Oxygenation-Induced Hemolysis: An In Vitro Study to Appraise Causative Factors 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In vitro hemolysis testing is commonly used to determine hemocompatibility of ExtraCorporeal Membrane Oxygenation (ECMO). However, poor reproducibility remains a challenging problem, due to several unidentified influencing factors. The present study investigated potential factors, such as flow rates, the use of anticoagulants, and gender of blood donors, which could play a role in hemolysis. Fresh human whole blood was anticoagulated with either citrate (n = 6) or heparin (n = 12; 6 female and 6 male blood donors). Blood was then circulated for 360 min at 4 L/min or 1.5 L/min. Regardless of flow rate conditions, hemolysis remained unchanged over time in citrated blood, but significantly increased after 240 min circulation in heparinized blood (<i<p</i< ≤ 0.01). The ratio of the normalized index of hemolysis (<i<NIH</i<) of heparinized blood to citrated blood was 11.7-fold higher at 4 L/min and 16.5–fold higher at 1.5 L/min. The difference in hemolysis between 1.5 L/min and 4 L/min concurred with findings of previous literature. In addition, the ratio of <i<NIH</i< of male heparinized blood to female was 1.7-fold higher at 4 L/min and 2.2-fold higher at 1.5 L/min. Our preliminary results suggested that the choice of anticoagulant and blood donor gender could be critical factors in hemolysis studies, and should be taken into account to improve testing reliability during ECMO. mechanical circulatory device blood trauma anticoagulants blood donor gender flow rate in vitro model Chemical technology Chemical engineering Katrina K. Ki verfasserin aut Meili Zhang verfasserin aut Cooper Asnicar verfasserin aut Hwa Jin Cho verfasserin aut Carmen Ainola verfasserin aut Mahe Bouquet verfasserin aut Silver Heinsar verfasserin aut Jo Philipp Pauls verfasserin aut Gianluigi Li Bassi verfasserin aut Jacky Suen verfasserin aut John F. Fraser verfasserin aut In Membranes MDPI AG, 2011 11(2021), 5, p 313 (DE-627)662495683 (DE-600)2614641-1 20770375 nnns volume:11 year:2021 number:5, p 313 https://doi.org/10.3390/membranes11050313 kostenfrei https://doaj.org/article/84053e8fbf7b463ba90550a2d76d5cbf kostenfrei https://www.mdpi.com/2077-0375/11/5/313 kostenfrei https://doaj.org/toc/2077-0375 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2021 5, p 313
allfieldsSound	10.3390/membranes11050313 doi (DE-627)DOAJ015079651 (DE-599)DOAJ84053e8fbf7b463ba90550a2d76d5cbf DE-627 ger DE-627 rakwb eng TP1-1185 TP155-156 Chris Hoi Houng Chan verfasserin aut Extracorporeal Membrane Oxygenation-Induced Hemolysis: An In Vitro Study to Appraise Causative Factors 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In vitro hemolysis testing is commonly used to determine hemocompatibility of ExtraCorporeal Membrane Oxygenation (ECMO). However, poor reproducibility remains a challenging problem, due to several unidentified influencing factors. The present study investigated potential factors, such as flow rates, the use of anticoagulants, and gender of blood donors, which could play a role in hemolysis. Fresh human whole blood was anticoagulated with either citrate (n = 6) or heparin (n = 12; 6 female and 6 male blood donors). Blood was then circulated for 360 min at 4 L/min or 1.5 L/min. Regardless of flow rate conditions, hemolysis remained unchanged over time in citrated blood, but significantly increased after 240 min circulation in heparinized blood (<i<p</i< ≤ 0.01). The ratio of the normalized index of hemolysis (<i<NIH</i<) of heparinized blood to citrated blood was 11.7-fold higher at 4 L/min and 16.5–fold higher at 1.5 L/min. The difference in hemolysis between 1.5 L/min and 4 L/min concurred with findings of previous literature. In addition, the ratio of <i<NIH</i< of male heparinized blood to female was 1.7-fold higher at 4 L/min and 2.2-fold higher at 1.5 L/min. Our preliminary results suggested that the choice of anticoagulant and blood donor gender could be critical factors in hemolysis studies, and should be taken into account to improve testing reliability during ECMO. mechanical circulatory device blood trauma anticoagulants blood donor gender flow rate in vitro model Chemical technology Chemical engineering Katrina K. Ki verfasserin aut Meili Zhang verfasserin aut Cooper Asnicar verfasserin aut Hwa Jin Cho verfasserin aut Carmen Ainola verfasserin aut Mahe Bouquet verfasserin aut Silver Heinsar verfasserin aut Jo Philipp Pauls verfasserin aut Gianluigi Li Bassi verfasserin aut Jacky Suen verfasserin aut John F. Fraser verfasserin aut In Membranes MDPI AG, 2011 11(2021), 5, p 313 (DE-627)662495683 (DE-600)2614641-1 20770375 nnns volume:11 year:2021 number:5, p 313 https://doi.org/10.3390/membranes11050313 kostenfrei https://doaj.org/article/84053e8fbf7b463ba90550a2d76d5cbf kostenfrei https://www.mdpi.com/2077-0375/11/5/313 kostenfrei https://doaj.org/toc/2077-0375 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2021 5, p 313
language	English
source	In Membranes 11(2021), 5, p 313 volume:11 year:2021 number:5, p 313
sourceStr	In Membranes 11(2021), 5, p 313 volume:11 year:2021 number:5, p 313
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	mechanical circulatory device blood trauma anticoagulants blood donor gender flow rate in vitro model Chemical technology Chemical engineering
isfreeaccess_bool	true
container_title	Membranes
authorswithroles_txt_mv	Chris Hoi Houng Chan @@aut@@ Katrina K. Ki @@aut@@ Meili Zhang @@aut@@ Cooper Asnicar @@aut@@ Hwa Jin Cho @@aut@@ Carmen Ainola @@aut@@ Mahe Bouquet @@aut@@ Silver Heinsar @@aut@@ Jo Philipp Pauls @@aut@@ Gianluigi Li Bassi @@aut@@ Jacky Suen @@aut@@ John F. Fraser @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	662495683
id	DOAJ015079651
language_de	englisch
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callnumber-first	T - Technology
author	Chris Hoi Houng Chan
spellingShingle	Chris Hoi Houng Chan misc TP1-1185 misc TP155-156 misc mechanical circulatory device misc blood trauma misc anticoagulants misc blood donor gender misc flow rate misc in vitro model misc Chemical technology misc Chemical engineering Extracorporeal Membrane Oxygenation-Induced Hemolysis: An In Vitro Study to Appraise Causative Factors
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topic_title	TP1-1185 TP155-156 Extracorporeal Membrane Oxygenation-Induced Hemolysis: An In Vitro Study to Appraise Causative Factors mechanical circulatory device blood trauma anticoagulants blood donor gender flow rate in vitro model
topic	misc TP1-1185 misc TP155-156 misc mechanical circulatory device misc blood trauma misc anticoagulants misc blood donor gender misc flow rate misc in vitro model misc Chemical technology misc Chemical engineering
topic_unstemmed	misc TP1-1185 misc TP155-156 misc mechanical circulatory device misc blood trauma misc anticoagulants misc blood donor gender misc flow rate misc in vitro model misc Chemical technology misc Chemical engineering
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title	Extracorporeal Membrane Oxygenation-Induced Hemolysis: An In Vitro Study to Appraise Causative Factors
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title_full	Extracorporeal Membrane Oxygenation-Induced Hemolysis: An In Vitro Study to Appraise Causative Factors
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title_sort	extracorporeal membrane oxygenation-induced hemolysis: an in vitro study to appraise causative factors
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title_auth	Extracorporeal Membrane Oxygenation-Induced Hemolysis: An In Vitro Study to Appraise Causative Factors
abstract	In vitro hemolysis testing is commonly used to determine hemocompatibility of ExtraCorporeal Membrane Oxygenation (ECMO). However, poor reproducibility remains a challenging problem, due to several unidentified influencing factors. The present study investigated potential factors, such as flow rates, the use of anticoagulants, and gender of blood donors, which could play a role in hemolysis. Fresh human whole blood was anticoagulated with either citrate (n = 6) or heparin (n = 12; 6 female and 6 male blood donors). Blood was then circulated for 360 min at 4 L/min or 1.5 L/min. Regardless of flow rate conditions, hemolysis remained unchanged over time in citrated blood, but significantly increased after 240 min circulation in heparinized blood (<i<p</i< ≤ 0.01). The ratio of the normalized index of hemolysis (<i<NIH</i<) of heparinized blood to citrated blood was 11.7-fold higher at 4 L/min and 16.5–fold higher at 1.5 L/min. The difference in hemolysis between 1.5 L/min and 4 L/min concurred with findings of previous literature. In addition, the ratio of <i<NIH</i< of male heparinized blood to female was 1.7-fold higher at 4 L/min and 2.2-fold higher at 1.5 L/min. Our preliminary results suggested that the choice of anticoagulant and blood donor gender could be critical factors in hemolysis studies, and should be taken into account to improve testing reliability during ECMO.
abstractGer	In vitro hemolysis testing is commonly used to determine hemocompatibility of ExtraCorporeal Membrane Oxygenation (ECMO). However, poor reproducibility remains a challenging problem, due to several unidentified influencing factors. The present study investigated potential factors, such as flow rates, the use of anticoagulants, and gender of blood donors, which could play a role in hemolysis. Fresh human whole blood was anticoagulated with either citrate (n = 6) or heparin (n = 12; 6 female and 6 male blood donors). Blood was then circulated for 360 min at 4 L/min or 1.5 L/min. Regardless of flow rate conditions, hemolysis remained unchanged over time in citrated blood, but significantly increased after 240 min circulation in heparinized blood (<i<p</i< ≤ 0.01). The ratio of the normalized index of hemolysis (<i<NIH</i<) of heparinized blood to citrated blood was 11.7-fold higher at 4 L/min and 16.5–fold higher at 1.5 L/min. The difference in hemolysis between 1.5 L/min and 4 L/min concurred with findings of previous literature. In addition, the ratio of <i<NIH</i< of male heparinized blood to female was 1.7-fold higher at 4 L/min and 2.2-fold higher at 1.5 L/min. Our preliminary results suggested that the choice of anticoagulant and blood donor gender could be critical factors in hemolysis studies, and should be taken into account to improve testing reliability during ECMO.
abstract_unstemmed	In vitro hemolysis testing is commonly used to determine hemocompatibility of ExtraCorporeal Membrane Oxygenation (ECMO). However, poor reproducibility remains a challenging problem, due to several unidentified influencing factors. The present study investigated potential factors, such as flow rates, the use of anticoagulants, and gender of blood donors, which could play a role in hemolysis. Fresh human whole blood was anticoagulated with either citrate (n = 6) or heparin (n = 12; 6 female and 6 male blood donors). Blood was then circulated for 360 min at 4 L/min or 1.5 L/min. Regardless of flow rate conditions, hemolysis remained unchanged over time in citrated blood, but significantly increased after 240 min circulation in heparinized blood (<i<p</i< ≤ 0.01). The ratio of the normalized index of hemolysis (<i<NIH</i<) of heparinized blood to citrated blood was 11.7-fold higher at 4 L/min and 16.5–fold higher at 1.5 L/min. The difference in hemolysis between 1.5 L/min and 4 L/min concurred with findings of previous literature. In addition, the ratio of <i<NIH</i< of male heparinized blood to female was 1.7-fold higher at 4 L/min and 2.2-fold higher at 1.5 L/min. Our preliminary results suggested that the choice of anticoagulant and blood donor gender could be critical factors in hemolysis studies, and should be taken into account to improve testing reliability during ECMO.
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container_issue	5, p 313
title_short	Extracorporeal Membrane Oxygenation-Induced Hemolysis: An In Vitro Study to Appraise Causative Factors
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