Role of long non-coding RNA-adducin 3 antisense RNA1 in liver fibrosis of biliary atresia

Biliary atresia (BA) is a devastating liver disease in neonates. Liver fibrosis is regarded as a universal and prominent feature of BA. Studies have revealed that long non-coding RNAs (lncRNAs) regulate cellular processes during the development of liver fibrosis in BA. Long non-coding RNA-adducin 3...
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Autor*in:	Yongqin Ye [verfasserIn] Weifang Wu [verfasserIn] Jiachen Zheng [verfasserIn] Lihui Zhang [verfasserIn] Bin Wang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Long non-coding RNA lnc-ADD3-AS1 LX-2 cell proliferation LX-2 cell migration liver fibrosis biliary atresia

Übergeordnetes Werk:	In: Bioengineered - Taylor & Francis Group, 2019, 13(2022), 3, Seite 6222-6230
Übergeordnetes Werk:	volume:13 ; year:2022 ; number:3 ; pages:6222-6230

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.1080/21655979.2022.2041321

Katalog-ID:	DOAJ015263800

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520			\|a Biliary atresia (BA) is a devastating liver disease in neonates. Liver fibrosis is regarded as a universal and prominent feature of BA. Studies have revealed that long non-coding RNAs (lncRNAs) regulate cellular processes during the development of liver fibrosis in BA. Long non-coding RNA-adducin 3 antisense RNA1 (lnc-ADD3-AS1) has been shown to increase susceptibility to BA. However, the role of lnc-ADD3-AS1 in liver fibrosis in BA remains unclear. Here, we investigated the role of lnc-ADD3-AS1 in the proliferation, migration, and apoptosis of the immortalized human hepatic stellate cell (HSC) line, LX-2. We successfully overexpressed and silenced lnc-ADD3-AS1 in LX-2 cells using adenovirus vectors and evaluated the proliferation of transfected cells using the Cell Counting Kit-8 (CCK8) assay. Cell apoptosis was detected using annexin V–fluorescein isothiocyanate (FITC)/propidium iodide (PI) double staining and flow cytometry. We then analyzed cell migration by performing wound-scratch and transwell migration assays. Our results show that lnc-ADD3-AS1 significantly promoted LX-2 cell proliferation and attenuated apoptosis. More importantly, lncRNA-ADD3-AS1 significantly accelerated the migration of LX-2 cells. Our data indicated that lncRNA-ADD3-AS1 plays a role in the pathogenesis of liver fibrosis in patients with BA and may serve as a potential diagnostic marker for monitoring liver fibrosis in BA or as a therapeutic target for the disease.
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allfields	10.1080/21655979.2022.2041321 doi (DE-627)DOAJ015263800 (DE-599)DOAJe4ea27ae54ac404cb7cf646e46f9e72b DE-627 ger DE-627 rakwb eng TP248.13-248.65 Yongqin Ye verfasserin aut Role of long non-coding RNA-adducin 3 antisense RNA1 in liver fibrosis of biliary atresia 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Biliary atresia (BA) is a devastating liver disease in neonates. Liver fibrosis is regarded as a universal and prominent feature of BA. Studies have revealed that long non-coding RNAs (lncRNAs) regulate cellular processes during the development of liver fibrosis in BA. Long non-coding RNA-adducin 3 antisense RNA1 (lnc-ADD3-AS1) has been shown to increase susceptibility to BA. However, the role of lnc-ADD3-AS1 in liver fibrosis in BA remains unclear. Here, we investigated the role of lnc-ADD3-AS1 in the proliferation, migration, and apoptosis of the immortalized human hepatic stellate cell (HSC) line, LX-2. We successfully overexpressed and silenced lnc-ADD3-AS1 in LX-2 cells using adenovirus vectors and evaluated the proliferation of transfected cells using the Cell Counting Kit-8 (CCK8) assay. Cell apoptosis was detected using annexin V–fluorescein isothiocyanate (FITC)/propidium iodide (PI) double staining and flow cytometry. We then analyzed cell migration by performing wound-scratch and transwell migration assays. Our results show that lnc-ADD3-AS1 significantly promoted LX-2 cell proliferation and attenuated apoptosis. More importantly, lncRNA-ADD3-AS1 significantly accelerated the migration of LX-2 cells. Our data indicated that lncRNA-ADD3-AS1 plays a role in the pathogenesis of liver fibrosis in patients with BA and may serve as a potential diagnostic marker for monitoring liver fibrosis in BA or as a therapeutic target for the disease. Long non-coding RNA lnc-ADD3-AS1 LX-2 cell proliferation LX-2 cell migration liver fibrosis biliary atresia Biotechnology Weifang Wu verfasserin aut Jiachen Zheng verfasserin aut Lihui Zhang verfasserin aut Bin Wang verfasserin aut In Bioengineered Taylor & Francis Group, 2019 13(2022), 3, Seite 6222-6230 (DE-627)770398189 (DE-600)2737830-5 21655987 nnns volume:13 year:2022 number:3 pages:6222-6230 https://doi.org/10.1080/21655979.2022.2041321 kostenfrei https://doaj.org/article/e4ea27ae54ac404cb7cf646e46f9e72b kostenfrei https://www.tandfonline.com/doi/10.1080/21655979.2022.2041321 kostenfrei https://doaj.org/toc/2165-5979 Journal toc kostenfrei https://doaj.org/toc/2165-5987 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 3 6222-6230
spelling	10.1080/21655979.2022.2041321 doi (DE-627)DOAJ015263800 (DE-599)DOAJe4ea27ae54ac404cb7cf646e46f9e72b DE-627 ger DE-627 rakwb eng TP248.13-248.65 Yongqin Ye verfasserin aut Role of long non-coding RNA-adducin 3 antisense RNA1 in liver fibrosis of biliary atresia 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Biliary atresia (BA) is a devastating liver disease in neonates. Liver fibrosis is regarded as a universal and prominent feature of BA. Studies have revealed that long non-coding RNAs (lncRNAs) regulate cellular processes during the development of liver fibrosis in BA. Long non-coding RNA-adducin 3 antisense RNA1 (lnc-ADD3-AS1) has been shown to increase susceptibility to BA. However, the role of lnc-ADD3-AS1 in liver fibrosis in BA remains unclear. Here, we investigated the role of lnc-ADD3-AS1 in the proliferation, migration, and apoptosis of the immortalized human hepatic stellate cell (HSC) line, LX-2. We successfully overexpressed and silenced lnc-ADD3-AS1 in LX-2 cells using adenovirus vectors and evaluated the proliferation of transfected cells using the Cell Counting Kit-8 (CCK8) assay. Cell apoptosis was detected using annexin V–fluorescein isothiocyanate (FITC)/propidium iodide (PI) double staining and flow cytometry. We then analyzed cell migration by performing wound-scratch and transwell migration assays. Our results show that lnc-ADD3-AS1 significantly promoted LX-2 cell proliferation and attenuated apoptosis. More importantly, lncRNA-ADD3-AS1 significantly accelerated the migration of LX-2 cells. Our data indicated that lncRNA-ADD3-AS1 plays a role in the pathogenesis of liver fibrosis in patients with BA and may serve as a potential diagnostic marker for monitoring liver fibrosis in BA or as a therapeutic target for the disease. Long non-coding RNA lnc-ADD3-AS1 LX-2 cell proliferation LX-2 cell migration liver fibrosis biliary atresia Biotechnology Weifang Wu verfasserin aut Jiachen Zheng verfasserin aut Lihui Zhang verfasserin aut Bin Wang verfasserin aut In Bioengineered Taylor & Francis Group, 2019 13(2022), 3, Seite 6222-6230 (DE-627)770398189 (DE-600)2737830-5 21655987 nnns volume:13 year:2022 number:3 pages:6222-6230 https://doi.org/10.1080/21655979.2022.2041321 kostenfrei https://doaj.org/article/e4ea27ae54ac404cb7cf646e46f9e72b kostenfrei https://www.tandfonline.com/doi/10.1080/21655979.2022.2041321 kostenfrei https://doaj.org/toc/2165-5979 Journal toc kostenfrei https://doaj.org/toc/2165-5987 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 3 6222-6230
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allfieldsSound	10.1080/21655979.2022.2041321 doi (DE-627)DOAJ015263800 (DE-599)DOAJe4ea27ae54ac404cb7cf646e46f9e72b DE-627 ger DE-627 rakwb eng TP248.13-248.65 Yongqin Ye verfasserin aut Role of long non-coding RNA-adducin 3 antisense RNA1 in liver fibrosis of biliary atresia 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Biliary atresia (BA) is a devastating liver disease in neonates. Liver fibrosis is regarded as a universal and prominent feature of BA. Studies have revealed that long non-coding RNAs (lncRNAs) regulate cellular processes during the development of liver fibrosis in BA. Long non-coding RNA-adducin 3 antisense RNA1 (lnc-ADD3-AS1) has been shown to increase susceptibility to BA. However, the role of lnc-ADD3-AS1 in liver fibrosis in BA remains unclear. Here, we investigated the role of lnc-ADD3-AS1 in the proliferation, migration, and apoptosis of the immortalized human hepatic stellate cell (HSC) line, LX-2. We successfully overexpressed and silenced lnc-ADD3-AS1 in LX-2 cells using adenovirus vectors and evaluated the proliferation of transfected cells using the Cell Counting Kit-8 (CCK8) assay. Cell apoptosis was detected using annexin V–fluorescein isothiocyanate (FITC)/propidium iodide (PI) double staining and flow cytometry. We then analyzed cell migration by performing wound-scratch and transwell migration assays. Our results show that lnc-ADD3-AS1 significantly promoted LX-2 cell proliferation and attenuated apoptosis. More importantly, lncRNA-ADD3-AS1 significantly accelerated the migration of LX-2 cells. Our data indicated that lncRNA-ADD3-AS1 plays a role in the pathogenesis of liver fibrosis in patients with BA and may serve as a potential diagnostic marker for monitoring liver fibrosis in BA or as a therapeutic target for the disease. Long non-coding RNA lnc-ADD3-AS1 LX-2 cell proliferation LX-2 cell migration liver fibrosis biliary atresia Biotechnology Weifang Wu verfasserin aut Jiachen Zheng verfasserin aut Lihui Zhang verfasserin aut Bin Wang verfasserin aut In Bioengineered Taylor & Francis Group, 2019 13(2022), 3, Seite 6222-6230 (DE-627)770398189 (DE-600)2737830-5 21655987 nnns volume:13 year:2022 number:3 pages:6222-6230 https://doi.org/10.1080/21655979.2022.2041321 kostenfrei https://doaj.org/article/e4ea27ae54ac404cb7cf646e46f9e72b kostenfrei https://www.tandfonline.com/doi/10.1080/21655979.2022.2041321 kostenfrei https://doaj.org/toc/2165-5979 Journal toc kostenfrei https://doaj.org/toc/2165-5987 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 3 6222-6230
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callnumber-first	T - Technology
author	Yongqin Ye
spellingShingle	Yongqin Ye misc TP248.13-248.65 misc Long non-coding RNA misc lnc-ADD3-AS1 misc LX-2 cell proliferation misc LX-2 cell migration misc liver fibrosis misc biliary atresia misc Biotechnology Role of long non-coding RNA-adducin 3 antisense RNA1 in liver fibrosis of biliary atresia
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topic_title	TP248.13-248.65 Role of long non-coding RNA-adducin 3 antisense RNA1 in liver fibrosis of biliary atresia Long non-coding RNA lnc-ADD3-AS1 LX-2 cell proliferation LX-2 cell migration liver fibrosis biliary atresia
topic	misc TP248.13-248.65 misc Long non-coding RNA misc lnc-ADD3-AS1 misc LX-2 cell proliferation misc LX-2 cell migration misc liver fibrosis misc biliary atresia misc Biotechnology
topic_unstemmed	misc TP248.13-248.65 misc Long non-coding RNA misc lnc-ADD3-AS1 misc LX-2 cell proliferation misc LX-2 cell migration misc liver fibrosis misc biliary atresia misc Biotechnology
topic_browse	misc TP248.13-248.65 misc Long non-coding RNA misc lnc-ADD3-AS1 misc LX-2 cell proliferation misc LX-2 cell migration misc liver fibrosis misc biliary atresia misc Biotechnology
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title	Role of long non-coding RNA-adducin 3 antisense RNA1 in liver fibrosis of biliary atresia
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title_full	Role of long non-coding RNA-adducin 3 antisense RNA1 in liver fibrosis of biliary atresia
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title_sort	role of long non-coding rna-adducin 3 antisense rna1 in liver fibrosis of biliary atresia
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title_auth	Role of long non-coding RNA-adducin 3 antisense RNA1 in liver fibrosis of biliary atresia
abstract	Biliary atresia (BA) is a devastating liver disease in neonates. Liver fibrosis is regarded as a universal and prominent feature of BA. Studies have revealed that long non-coding RNAs (lncRNAs) regulate cellular processes during the development of liver fibrosis in BA. Long non-coding RNA-adducin 3 antisense RNA1 (lnc-ADD3-AS1) has been shown to increase susceptibility to BA. However, the role of lnc-ADD3-AS1 in liver fibrosis in BA remains unclear. Here, we investigated the role of lnc-ADD3-AS1 in the proliferation, migration, and apoptosis of the immortalized human hepatic stellate cell (HSC) line, LX-2. We successfully overexpressed and silenced lnc-ADD3-AS1 in LX-2 cells using adenovirus vectors and evaluated the proliferation of transfected cells using the Cell Counting Kit-8 (CCK8) assay. Cell apoptosis was detected using annexin V–fluorescein isothiocyanate (FITC)/propidium iodide (PI) double staining and flow cytometry. We then analyzed cell migration by performing wound-scratch and transwell migration assays. Our results show that lnc-ADD3-AS1 significantly promoted LX-2 cell proliferation and attenuated apoptosis. More importantly, lncRNA-ADD3-AS1 significantly accelerated the migration of LX-2 cells. Our data indicated that lncRNA-ADD3-AS1 plays a role in the pathogenesis of liver fibrosis in patients with BA and may serve as a potential diagnostic marker for monitoring liver fibrosis in BA or as a therapeutic target for the disease.
abstractGer	Biliary atresia (BA) is a devastating liver disease in neonates. Liver fibrosis is regarded as a universal and prominent feature of BA. Studies have revealed that long non-coding RNAs (lncRNAs) regulate cellular processes during the development of liver fibrosis in BA. Long non-coding RNA-adducin 3 antisense RNA1 (lnc-ADD3-AS1) has been shown to increase susceptibility to BA. However, the role of lnc-ADD3-AS1 in liver fibrosis in BA remains unclear. Here, we investigated the role of lnc-ADD3-AS1 in the proliferation, migration, and apoptosis of the immortalized human hepatic stellate cell (HSC) line, LX-2. We successfully overexpressed and silenced lnc-ADD3-AS1 in LX-2 cells using adenovirus vectors and evaluated the proliferation of transfected cells using the Cell Counting Kit-8 (CCK8) assay. Cell apoptosis was detected using annexin V–fluorescein isothiocyanate (FITC)/propidium iodide (PI) double staining and flow cytometry. We then analyzed cell migration by performing wound-scratch and transwell migration assays. Our results show that lnc-ADD3-AS1 significantly promoted LX-2 cell proliferation and attenuated apoptosis. More importantly, lncRNA-ADD3-AS1 significantly accelerated the migration of LX-2 cells. Our data indicated that lncRNA-ADD3-AS1 plays a role in the pathogenesis of liver fibrosis in patients with BA and may serve as a potential diagnostic marker for monitoring liver fibrosis in BA or as a therapeutic target for the disease.
abstract_unstemmed	Biliary atresia (BA) is a devastating liver disease in neonates. Liver fibrosis is regarded as a universal and prominent feature of BA. Studies have revealed that long non-coding RNAs (lncRNAs) regulate cellular processes during the development of liver fibrosis in BA. Long non-coding RNA-adducin 3 antisense RNA1 (lnc-ADD3-AS1) has been shown to increase susceptibility to BA. However, the role of lnc-ADD3-AS1 in liver fibrosis in BA remains unclear. Here, we investigated the role of lnc-ADD3-AS1 in the proliferation, migration, and apoptosis of the immortalized human hepatic stellate cell (HSC) line, LX-2. We successfully overexpressed and silenced lnc-ADD3-AS1 in LX-2 cells using adenovirus vectors and evaluated the proliferation of transfected cells using the Cell Counting Kit-8 (CCK8) assay. Cell apoptosis was detected using annexin V–fluorescein isothiocyanate (FITC)/propidium iodide (PI) double staining and flow cytometry. We then analyzed cell migration by performing wound-scratch and transwell migration assays. Our results show that lnc-ADD3-AS1 significantly promoted LX-2 cell proliferation and attenuated apoptosis. More importantly, lncRNA-ADD3-AS1 significantly accelerated the migration of LX-2 cells. Our data indicated that lncRNA-ADD3-AS1 plays a role in the pathogenesis of liver fibrosis in patients with BA and may serve as a potential diagnostic marker for monitoring liver fibrosis in BA or as a therapeutic target for the disease.
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title_short	Role of long non-coding RNA-adducin 3 antisense RNA1 in liver fibrosis of biliary atresia
url	https://doi.org/10.1080/21655979.2022.2041321 https://doaj.org/article/e4ea27ae54ac404cb7cf646e46f9e72b https://www.tandfonline.com/doi/10.1080/21655979.2022.2041321 https://doaj.org/toc/2165-5979 https://doaj.org/toc/2165-5987
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up_date	2024-07-03T13:57:12.349Z
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Our results show that lnc-ADD3-AS1 significantly promoted LX-2 cell proliferation and attenuated apoptosis. More importantly, lncRNA-ADD3-AS1 significantly accelerated the migration of LX-2 cells. 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Role of long non-coding RNA-adducin 3 antisense RNA1 in liver fibrosis of biliary atresia

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