PPARγ Expression Is Diminished in Macrophages of Recurrent Miscarriage Placentas

PPARγ belongs to the group of nuclear receptors which is expressed in the trophoblast and together with other factors is responsible for the maintenance of pregnancy. Apart from that PPARγ is also a main factor for macrophage polarization. The aim of this study was to investigate...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Theresa Maria Kolben [verfasserIn] Elisabeth Rogatsch [verfasserIn] Aurelia Vattai [verfasserIn] Anna Hester [verfasserIn] Christina Kuhn [verfasserIn] Elisa Schmoeckel [verfasserIn] Sven Mahner [verfasserIn] Udo Jeschke [verfasserIn] Thomas Kolben [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	PPARγ first trimester placenta decidual macrophages miscarriage

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 19(2018), 7, p 1872
Übergeordnetes Werk:	volume:19 ; year:2018 ; number:7, p 1872

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/ijms19071872

Katalog-ID:	DOAJ015265048

Internformat


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allfields	10.3390/ijms19071872 doi (DE-627)DOAJ015265048 (DE-599)DOAJ0914b94e4d2644eba1fd84264ecc213a DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Theresa Maria Kolben verfasserin aut PPARγ Expression Is Diminished in Macrophages of Recurrent Miscarriage Placentas 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier PPARγ belongs to the group of nuclear receptors which is expressed in the trophoblast and together with other factors is responsible for the maintenance of pregnancy. Apart from that PPARγ is also a main factor for macrophage polarization. The aim of this study was to investigate the combined expression pattern and frequency of PPARγ under physiological circumstances and in spontaneous and recurrent miscarriages in the trophoblast and in maternal macrophages of the decidua. Human placental tissues of the first trimester (15 physiologic pregnancies, 15 spontaneous abortion and 16 recurrent miscarriage placentas) were analyzed for expression of the nuclear receptor PPARγ. Expression changes were evaluated by immunohistochemistry and real time PCR (RT-PCR) in trophoblast and in maternal macrophages of the decidua. Maternal macrophages were identified by double immunofluorescence using cluster of differentiation 68 (CD68) as marker for macrophages and further characterized regarding their M1/M2 polarization status. The intermediate villous trophoblast revealed a significantly lower PPARγ expression in spontaneous and recurrent abortion. Maternal macrophages express PPARγ. Their number is significantly enhanced in the decidua of spontaneous miscarriages whereas in recurrent miscarriages maternal macrophages seem to express PPARγ only in very few cases. PPARγ is associated with an M2 polarization state that is common for decidual macrophages. The lack of PPARγ in recurrent miscarriage decidual macrophages seems to be associated with a specific inflammatory response against the fetus. PPARγ first trimester placenta decidual macrophages miscarriage Biology (General) Chemistry Elisabeth Rogatsch verfasserin aut Aurelia Vattai verfasserin aut Anna Hester verfasserin aut Christina Kuhn verfasserin aut Elisa Schmoeckel verfasserin aut Sven Mahner verfasserin aut Udo Jeschke verfasserin aut Thomas Kolben verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 19(2018), 7, p 1872 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:19 year:2018 number:7, p 1872 https://doi.org/10.3390/ijms19071872 kostenfrei https://doaj.org/article/0914b94e4d2644eba1fd84264ecc213a kostenfrei http://www.mdpi.com/1422-0067/19/7/1872 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2018 7, p 1872
spelling	10.3390/ijms19071872 doi (DE-627)DOAJ015265048 (DE-599)DOAJ0914b94e4d2644eba1fd84264ecc213a DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Theresa Maria Kolben verfasserin aut PPARγ Expression Is Diminished in Macrophages of Recurrent Miscarriage Placentas 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier PPARγ belongs to the group of nuclear receptors which is expressed in the trophoblast and together with other factors is responsible for the maintenance of pregnancy. Apart from that PPARγ is also a main factor for macrophage polarization. The aim of this study was to investigate the combined expression pattern and frequency of PPARγ under physiological circumstances and in spontaneous and recurrent miscarriages in the trophoblast and in maternal macrophages of the decidua. Human placental tissues of the first trimester (15 physiologic pregnancies, 15 spontaneous abortion and 16 recurrent miscarriage placentas) were analyzed for expression of the nuclear receptor PPARγ. Expression changes were evaluated by immunohistochemistry and real time PCR (RT-PCR) in trophoblast and in maternal macrophages of the decidua. Maternal macrophages were identified by double immunofluorescence using cluster of differentiation 68 (CD68) as marker for macrophages and further characterized regarding their M1/M2 polarization status. The intermediate villous trophoblast revealed a significantly lower PPARγ expression in spontaneous and recurrent abortion. Maternal macrophages express PPARγ. Their number is significantly enhanced in the decidua of spontaneous miscarriages whereas in recurrent miscarriages maternal macrophages seem to express PPARγ only in very few cases. PPARγ is associated with an M2 polarization state that is common for decidual macrophages. The lack of PPARγ in recurrent miscarriage decidual macrophages seems to be associated with a specific inflammatory response against the fetus. PPARγ first trimester placenta decidual macrophages miscarriage Biology (General) Chemistry Elisabeth Rogatsch verfasserin aut Aurelia Vattai verfasserin aut Anna Hester verfasserin aut Christina Kuhn verfasserin aut Elisa Schmoeckel verfasserin aut Sven Mahner verfasserin aut Udo Jeschke verfasserin aut Thomas Kolben verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 19(2018), 7, p 1872 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:19 year:2018 number:7, p 1872 https://doi.org/10.3390/ijms19071872 kostenfrei https://doaj.org/article/0914b94e4d2644eba1fd84264ecc213a kostenfrei http://www.mdpi.com/1422-0067/19/7/1872 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2018 7, p 1872
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allfieldsGer	10.3390/ijms19071872 doi (DE-627)DOAJ015265048 (DE-599)DOAJ0914b94e4d2644eba1fd84264ecc213a DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Theresa Maria Kolben verfasserin aut PPARγ Expression Is Diminished in Macrophages of Recurrent Miscarriage Placentas 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier PPARγ belongs to the group of nuclear receptors which is expressed in the trophoblast and together with other factors is responsible for the maintenance of pregnancy. Apart from that PPARγ is also a main factor for macrophage polarization. The aim of this study was to investigate the combined expression pattern and frequency of PPARγ under physiological circumstances and in spontaneous and recurrent miscarriages in the trophoblast and in maternal macrophages of the decidua. Human placental tissues of the first trimester (15 physiologic pregnancies, 15 spontaneous abortion and 16 recurrent miscarriage placentas) were analyzed for expression of the nuclear receptor PPARγ. Expression changes were evaluated by immunohistochemistry and real time PCR (RT-PCR) in trophoblast and in maternal macrophages of the decidua. Maternal macrophages were identified by double immunofluorescence using cluster of differentiation 68 (CD68) as marker for macrophages and further characterized regarding their M1/M2 polarization status. The intermediate villous trophoblast revealed a significantly lower PPARγ expression in spontaneous and recurrent abortion. Maternal macrophages express PPARγ. Their number is significantly enhanced in the decidua of spontaneous miscarriages whereas in recurrent miscarriages maternal macrophages seem to express PPARγ only in very few cases. PPARγ is associated with an M2 polarization state that is common for decidual macrophages. The lack of PPARγ in recurrent miscarriage decidual macrophages seems to be associated with a specific inflammatory response against the fetus. PPARγ first trimester placenta decidual macrophages miscarriage Biology (General) Chemistry Elisabeth Rogatsch verfasserin aut Aurelia Vattai verfasserin aut Anna Hester verfasserin aut Christina Kuhn verfasserin aut Elisa Schmoeckel verfasserin aut Sven Mahner verfasserin aut Udo Jeschke verfasserin aut Thomas Kolben verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 19(2018), 7, p 1872 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:19 year:2018 number:7, p 1872 https://doi.org/10.3390/ijms19071872 kostenfrei https://doaj.org/article/0914b94e4d2644eba1fd84264ecc213a kostenfrei http://www.mdpi.com/1422-0067/19/7/1872 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2018 7, p 1872
allfieldsSound	10.3390/ijms19071872 doi (DE-627)DOAJ015265048 (DE-599)DOAJ0914b94e4d2644eba1fd84264ecc213a DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Theresa Maria Kolben verfasserin aut PPARγ Expression Is Diminished in Macrophages of Recurrent Miscarriage Placentas 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier PPARγ belongs to the group of nuclear receptors which is expressed in the trophoblast and together with other factors is responsible for the maintenance of pregnancy. Apart from that PPARγ is also a main factor for macrophage polarization. The aim of this study was to investigate the combined expression pattern and frequency of PPARγ under physiological circumstances and in spontaneous and recurrent miscarriages in the trophoblast and in maternal macrophages of the decidua. Human placental tissues of the first trimester (15 physiologic pregnancies, 15 spontaneous abortion and 16 recurrent miscarriage placentas) were analyzed for expression of the nuclear receptor PPARγ. Expression changes were evaluated by immunohistochemistry and real time PCR (RT-PCR) in trophoblast and in maternal macrophages of the decidua. Maternal macrophages were identified by double immunofluorescence using cluster of differentiation 68 (CD68) as marker for macrophages and further characterized regarding their M1/M2 polarization status. The intermediate villous trophoblast revealed a significantly lower PPARγ expression in spontaneous and recurrent abortion. Maternal macrophages express PPARγ. Their number is significantly enhanced in the decidua of spontaneous miscarriages whereas in recurrent miscarriages maternal macrophages seem to express PPARγ only in very few cases. PPARγ is associated with an M2 polarization state that is common for decidual macrophages. The lack of PPARγ in recurrent miscarriage decidual macrophages seems to be associated with a specific inflammatory response against the fetus. PPARγ first trimester placenta decidual macrophages miscarriage Biology (General) Chemistry Elisabeth Rogatsch verfasserin aut Aurelia Vattai verfasserin aut Anna Hester verfasserin aut Christina Kuhn verfasserin aut Elisa Schmoeckel verfasserin aut Sven Mahner verfasserin aut Udo Jeschke verfasserin aut Thomas Kolben verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 19(2018), 7, p 1872 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:19 year:2018 number:7, p 1872 https://doi.org/10.3390/ijms19071872 kostenfrei https://doaj.org/article/0914b94e4d2644eba1fd84264ecc213a kostenfrei http://www.mdpi.com/1422-0067/19/7/1872 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2018 7, p 1872
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source	In International Journal of Molecular Sciences 19(2018), 7, p 1872 volume:19 year:2018 number:7, p 1872
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callnumber-first	Q - Science
author	Theresa Maria Kolben
spellingShingle	Theresa Maria Kolben misc QH301-705.5 misc QD1-999 misc PPARγ misc first trimester placenta misc decidual macrophages misc miscarriage misc Biology (General) misc Chemistry PPARγ Expression Is Diminished in Macrophages of Recurrent Miscarriage Placentas
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topic_title	QH301-705.5 QD1-999 PPARγ Expression Is Diminished in Macrophages of Recurrent Miscarriage Placentas PPARγ first trimester placenta decidual macrophages miscarriage
topic	misc QH301-705.5 misc QD1-999 misc PPARγ misc first trimester placenta misc decidual macrophages misc miscarriage misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc PPARγ misc first trimester placenta misc decidual macrophages misc miscarriage misc Biology (General) misc Chemistry
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title	PPARγ Expression Is Diminished in Macrophages of Recurrent Miscarriage Placentas
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title_full	PPARγ Expression Is Diminished in Macrophages of Recurrent Miscarriage Placentas
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title_sort	pparγ expression is diminished in macrophages of recurrent miscarriage placentas
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title_auth	PPARγ Expression Is Diminished in Macrophages of Recurrent Miscarriage Placentas
abstract	PPARγ belongs to the group of nuclear receptors which is expressed in the trophoblast and together with other factors is responsible for the maintenance of pregnancy. Apart from that PPARγ is also a main factor for macrophage polarization. The aim of this study was to investigate the combined expression pattern and frequency of PPARγ under physiological circumstances and in spontaneous and recurrent miscarriages in the trophoblast and in maternal macrophages of the decidua. Human placental tissues of the first trimester (15 physiologic pregnancies, 15 spontaneous abortion and 16 recurrent miscarriage placentas) were analyzed for expression of the nuclear receptor PPARγ. Expression changes were evaluated by immunohistochemistry and real time PCR (RT-PCR) in trophoblast and in maternal macrophages of the decidua. Maternal macrophages were identified by double immunofluorescence using cluster of differentiation 68 (CD68) as marker for macrophages and further characterized regarding their M1/M2 polarization status. The intermediate villous trophoblast revealed a significantly lower PPARγ expression in spontaneous and recurrent abortion. Maternal macrophages express PPARγ. Their number is significantly enhanced in the decidua of spontaneous miscarriages whereas in recurrent miscarriages maternal macrophages seem to express PPARγ only in very few cases. PPARγ is associated with an M2 polarization state that is common for decidual macrophages. The lack of PPARγ in recurrent miscarriage decidual macrophages seems to be associated with a specific inflammatory response against the fetus.
abstractGer	PPARγ belongs to the group of nuclear receptors which is expressed in the trophoblast and together with other factors is responsible for the maintenance of pregnancy. Apart from that PPARγ is also a main factor for macrophage polarization. The aim of this study was to investigate the combined expression pattern and frequency of PPARγ under physiological circumstances and in spontaneous and recurrent miscarriages in the trophoblast and in maternal macrophages of the decidua. Human placental tissues of the first trimester (15 physiologic pregnancies, 15 spontaneous abortion and 16 recurrent miscarriage placentas) were analyzed for expression of the nuclear receptor PPARγ. Expression changes were evaluated by immunohistochemistry and real time PCR (RT-PCR) in trophoblast and in maternal macrophages of the decidua. Maternal macrophages were identified by double immunofluorescence using cluster of differentiation 68 (CD68) as marker for macrophages and further characterized regarding their M1/M2 polarization status. The intermediate villous trophoblast revealed a significantly lower PPARγ expression in spontaneous and recurrent abortion. Maternal macrophages express PPARγ. Their number is significantly enhanced in the decidua of spontaneous miscarriages whereas in recurrent miscarriages maternal macrophages seem to express PPARγ only in very few cases. PPARγ is associated with an M2 polarization state that is common for decidual macrophages. The lack of PPARγ in recurrent miscarriage decidual macrophages seems to be associated with a specific inflammatory response against the fetus.
abstract_unstemmed	PPARγ belongs to the group of nuclear receptors which is expressed in the trophoblast and together with other factors is responsible for the maintenance of pregnancy. Apart from that PPARγ is also a main factor for macrophage polarization. The aim of this study was to investigate the combined expression pattern and frequency of PPARγ under physiological circumstances and in spontaneous and recurrent miscarriages in the trophoblast and in maternal macrophages of the decidua. Human placental tissues of the first trimester (15 physiologic pregnancies, 15 spontaneous abortion and 16 recurrent miscarriage placentas) were analyzed for expression of the nuclear receptor PPARγ. Expression changes were evaluated by immunohistochemistry and real time PCR (RT-PCR) in trophoblast and in maternal macrophages of the decidua. Maternal macrophages were identified by double immunofluorescence using cluster of differentiation 68 (CD68) as marker for macrophages and further characterized regarding their M1/M2 polarization status. The intermediate villous trophoblast revealed a significantly lower PPARγ expression in spontaneous and recurrent abortion. Maternal macrophages express PPARγ. Their number is significantly enhanced in the decidua of spontaneous miscarriages whereas in recurrent miscarriages maternal macrophages seem to express PPARγ only in very few cases. PPARγ is associated with an M2 polarization state that is common for decidual macrophages. The lack of PPARγ in recurrent miscarriage decidual macrophages seems to be associated with a specific inflammatory response against the fetus.
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title_short	PPARγ Expression Is Diminished in Macrophages of Recurrent Miscarriage Placentas
url	https://doi.org/10.3390/ijms19071872 https://doaj.org/article/0914b94e4d2644eba1fd84264ecc213a http://www.mdpi.com/1422-0067/19/7/1872 https://doaj.org/toc/1422-0067
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up_date	2024-07-03T13:57:38.258Z
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The intermediate villous trophoblast revealed a significantly lower PPAR&gamma; expression in spontaneous and recurrent abortion. Maternal macrophages express PPAR&gamma;. Their number is significantly enhanced in the decidua of spontaneous miscarriages whereas in recurrent miscarriages maternal macrophages seem to express PPAR&gamma; only in very few cases. PPAR&gamma; is associated with an M2 polarization state that is common for decidual macrophages. 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PPARγ Expression Is Diminished in Macrophages of Recurrent Miscarriage Placentas

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