Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content

Current analysis of exosomes focuses primarily on bulk analysis, where exosome-to-exosome variability cannot be assessed. In this study, we used Raman spectroscopy to study the chemical composition of single exosomes. We measured spectra of individual exosomes from 8 cell lines. Cell-line-averaged s...
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Autor*in:	Zachary J. Smith [verfasserIn] Changwon Lee [verfasserIn] Tatu Rojalin [verfasserIn] Randy P. Carney [verfasserIn] Sidhartha Hazari [verfasserIn] Alisha Knudson [verfasserIn] Kit Lam [verfasserIn] Heikki Saari [verfasserIn] Elisa Lazaro Ibañez [verfasserIn] Tapani Viitala [verfasserIn] Timo Laaksonen [verfasserIn] Marjo Yliperttula [verfasserIn] Sebastian Wachsmann-Hogiu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	exosomes microvesicles single particle Raman laser trap membrane content

Übergeordnetes Werk:	In: Journal of Extracellular Vesicles - Wiley, 2012, 4(2015), 0, Seite 15
Übergeordnetes Werk:	volume:4 ; year:2015 ; number:0 ; pages:15

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3402/jev.v4.28533

Katalog-ID:	DOAJ01538215X

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520			\|a Current analysis of exosomes focuses primarily on bulk analysis, where exosome-to-exosome variability cannot be assessed. In this study, we used Raman spectroscopy to study the chemical composition of single exosomes. We measured spectra of individual exosomes from 8 cell lines. Cell-line-averaged spectra varied considerably, reflecting the variation in total exosomal protein, lipid, genetic, and cytosolic content. Unexpectedly, single exosomes isolated from the same cell type also exhibited high spectral variability. Subsequent spectral analysis revealed clustering of single exosomes into 4 distinct groups that were not cell-line specific. Each group contained exosomes from multiple cell lines, and most cell lines had exosomes in multiple groups. The differences between these groups are related to chemical differences primarily due to differing membrane composition. Through a principal components analysis, we identified that the major sources of spectral variation among the exosomes were in cholesterol content, relative expression of phospholipids to cholesterol, and surface protein expression. For example, exosomes derived from cancerous versus non-cancerous cell lines can be largely separated based on their relative expression of cholesterol and phospholipids. We are the first to indicate that exosome subpopulations are shared among cell types, suggesting distributed exosome functionality. The origins of these differences are likely related to the specific role of extracellular vesicle subpopulations in both normal cell function and carcinogenesis, and they may provide diagnostic potential at the single exosome level.
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allfields	10.3402/jev.v4.28533 doi (DE-627)DOAJ01538215X (DE-599)DOAJc8a961dc22c84fe0883483f6f400f53e DE-627 ger DE-627 rakwb eng QH573-671 Zachary J. Smith verfasserin aut Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Current analysis of exosomes focuses primarily on bulk analysis, where exosome-to-exosome variability cannot be assessed. In this study, we used Raman spectroscopy to study the chemical composition of single exosomes. We measured spectra of individual exosomes from 8 cell lines. Cell-line-averaged spectra varied considerably, reflecting the variation in total exosomal protein, lipid, genetic, and cytosolic content. Unexpectedly, single exosomes isolated from the same cell type also exhibited high spectral variability. Subsequent spectral analysis revealed clustering of single exosomes into 4 distinct groups that were not cell-line specific. Each group contained exosomes from multiple cell lines, and most cell lines had exosomes in multiple groups. The differences between these groups are related to chemical differences primarily due to differing membrane composition. Through a principal components analysis, we identified that the major sources of spectral variation among the exosomes were in cholesterol content, relative expression of phospholipids to cholesterol, and surface protein expression. For example, exosomes derived from cancerous versus non-cancerous cell lines can be largely separated based on their relative expression of cholesterol and phospholipids. We are the first to indicate that exosome subpopulations are shared among cell types, suggesting distributed exosome functionality. The origins of these differences are likely related to the specific role of extracellular vesicle subpopulations in both normal cell function and carcinogenesis, and they may provide diagnostic potential at the single exosome level. exosomes microvesicles single particle Raman laser trap membrane content Cytology Changwon Lee verfasserin aut Tatu Rojalin verfasserin aut Randy P. Carney verfasserin aut Sidhartha Hazari verfasserin aut Alisha Knudson verfasserin aut Kit Lam verfasserin aut Heikki Saari verfasserin aut Elisa Lazaro Ibañez verfasserin aut Tapani Viitala verfasserin aut Timo Laaksonen verfasserin aut Marjo Yliperttula verfasserin aut Sebastian Wachsmann-Hogiu verfasserin aut In Journal of Extracellular Vesicles Wiley, 2012 4(2015), 0, Seite 15 (DE-627)726923710 (DE-600)2683797-3 20013078 nnns volume:4 year:2015 number:0 pages:15 https://doi.org/10.3402/jev.v4.28533 kostenfrei https://doaj.org/article/c8a961dc22c84fe0883483f6f400f53e kostenfrei http://www.journalofextracellularvesicles.net/index.php/jev/article/view/28533/pdf_39 kostenfrei https://doaj.org/toc/2001-3078 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2015 0 15
spelling	10.3402/jev.v4.28533 doi (DE-627)DOAJ01538215X (DE-599)DOAJc8a961dc22c84fe0883483f6f400f53e DE-627 ger DE-627 rakwb eng QH573-671 Zachary J. Smith verfasserin aut Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Current analysis of exosomes focuses primarily on bulk analysis, where exosome-to-exosome variability cannot be assessed. In this study, we used Raman spectroscopy to study the chemical composition of single exosomes. We measured spectra of individual exosomes from 8 cell lines. Cell-line-averaged spectra varied considerably, reflecting the variation in total exosomal protein, lipid, genetic, and cytosolic content. Unexpectedly, single exosomes isolated from the same cell type also exhibited high spectral variability. Subsequent spectral analysis revealed clustering of single exosomes into 4 distinct groups that were not cell-line specific. Each group contained exosomes from multiple cell lines, and most cell lines had exosomes in multiple groups. The differences between these groups are related to chemical differences primarily due to differing membrane composition. Through a principal components analysis, we identified that the major sources of spectral variation among the exosomes were in cholesterol content, relative expression of phospholipids to cholesterol, and surface protein expression. For example, exosomes derived from cancerous versus non-cancerous cell lines can be largely separated based on their relative expression of cholesterol and phospholipids. We are the first to indicate that exosome subpopulations are shared among cell types, suggesting distributed exosome functionality. The origins of these differences are likely related to the specific role of extracellular vesicle subpopulations in both normal cell function and carcinogenesis, and they may provide diagnostic potential at the single exosome level. exosomes microvesicles single particle Raman laser trap membrane content Cytology Changwon Lee verfasserin aut Tatu Rojalin verfasserin aut Randy P. Carney verfasserin aut Sidhartha Hazari verfasserin aut Alisha Knudson verfasserin aut Kit Lam verfasserin aut Heikki Saari verfasserin aut Elisa Lazaro Ibañez verfasserin aut Tapani Viitala verfasserin aut Timo Laaksonen verfasserin aut Marjo Yliperttula verfasserin aut Sebastian Wachsmann-Hogiu verfasserin aut In Journal of Extracellular Vesicles Wiley, 2012 4(2015), 0, Seite 15 (DE-627)726923710 (DE-600)2683797-3 20013078 nnns volume:4 year:2015 number:0 pages:15 https://doi.org/10.3402/jev.v4.28533 kostenfrei https://doaj.org/article/c8a961dc22c84fe0883483f6f400f53e kostenfrei http://www.journalofextracellularvesicles.net/index.php/jev/article/view/28533/pdf_39 kostenfrei https://doaj.org/toc/2001-3078 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2015 0 15
allfields_unstemmed	10.3402/jev.v4.28533 doi (DE-627)DOAJ01538215X (DE-599)DOAJc8a961dc22c84fe0883483f6f400f53e DE-627 ger DE-627 rakwb eng QH573-671 Zachary J. Smith verfasserin aut Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Current analysis of exosomes focuses primarily on bulk analysis, where exosome-to-exosome variability cannot be assessed. In this study, we used Raman spectroscopy to study the chemical composition of single exosomes. We measured spectra of individual exosomes from 8 cell lines. Cell-line-averaged spectra varied considerably, reflecting the variation in total exosomal protein, lipid, genetic, and cytosolic content. Unexpectedly, single exosomes isolated from the same cell type also exhibited high spectral variability. Subsequent spectral analysis revealed clustering of single exosomes into 4 distinct groups that were not cell-line specific. Each group contained exosomes from multiple cell lines, and most cell lines had exosomes in multiple groups. The differences between these groups are related to chemical differences primarily due to differing membrane composition. Through a principal components analysis, we identified that the major sources of spectral variation among the exosomes were in cholesterol content, relative expression of phospholipids to cholesterol, and surface protein expression. For example, exosomes derived from cancerous versus non-cancerous cell lines can be largely separated based on their relative expression of cholesterol and phospholipids. We are the first to indicate that exosome subpopulations are shared among cell types, suggesting distributed exosome functionality. The origins of these differences are likely related to the specific role of extracellular vesicle subpopulations in both normal cell function and carcinogenesis, and they may provide diagnostic potential at the single exosome level. exosomes microvesicles single particle Raman laser trap membrane content Cytology Changwon Lee verfasserin aut Tatu Rojalin verfasserin aut Randy P. Carney verfasserin aut Sidhartha Hazari verfasserin aut Alisha Knudson verfasserin aut Kit Lam verfasserin aut Heikki Saari verfasserin aut Elisa Lazaro Ibañez verfasserin aut Tapani Viitala verfasserin aut Timo Laaksonen verfasserin aut Marjo Yliperttula verfasserin aut Sebastian Wachsmann-Hogiu verfasserin aut In Journal of Extracellular Vesicles Wiley, 2012 4(2015), 0, Seite 15 (DE-627)726923710 (DE-600)2683797-3 20013078 nnns volume:4 year:2015 number:0 pages:15 https://doi.org/10.3402/jev.v4.28533 kostenfrei https://doaj.org/article/c8a961dc22c84fe0883483f6f400f53e kostenfrei http://www.journalofextracellularvesicles.net/index.php/jev/article/view/28533/pdf_39 kostenfrei https://doaj.org/toc/2001-3078 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2015 0 15
allfieldsGer	10.3402/jev.v4.28533 doi (DE-627)DOAJ01538215X (DE-599)DOAJc8a961dc22c84fe0883483f6f400f53e DE-627 ger DE-627 rakwb eng QH573-671 Zachary J. Smith verfasserin aut Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Current analysis of exosomes focuses primarily on bulk analysis, where exosome-to-exosome variability cannot be assessed. In this study, we used Raman spectroscopy to study the chemical composition of single exosomes. We measured spectra of individual exosomes from 8 cell lines. Cell-line-averaged spectra varied considerably, reflecting the variation in total exosomal protein, lipid, genetic, and cytosolic content. Unexpectedly, single exosomes isolated from the same cell type also exhibited high spectral variability. Subsequent spectral analysis revealed clustering of single exosomes into 4 distinct groups that were not cell-line specific. Each group contained exosomes from multiple cell lines, and most cell lines had exosomes in multiple groups. The differences between these groups are related to chemical differences primarily due to differing membrane composition. Through a principal components analysis, we identified that the major sources of spectral variation among the exosomes were in cholesterol content, relative expression of phospholipids to cholesterol, and surface protein expression. For example, exosomes derived from cancerous versus non-cancerous cell lines can be largely separated based on their relative expression of cholesterol and phospholipids. We are the first to indicate that exosome subpopulations are shared among cell types, suggesting distributed exosome functionality. The origins of these differences are likely related to the specific role of extracellular vesicle subpopulations in both normal cell function and carcinogenesis, and they may provide diagnostic potential at the single exosome level. exosomes microvesicles single particle Raman laser trap membrane content Cytology Changwon Lee verfasserin aut Tatu Rojalin verfasserin aut Randy P. Carney verfasserin aut Sidhartha Hazari verfasserin aut Alisha Knudson verfasserin aut Kit Lam verfasserin aut Heikki Saari verfasserin aut Elisa Lazaro Ibañez verfasserin aut Tapani Viitala verfasserin aut Timo Laaksonen verfasserin aut Marjo Yliperttula verfasserin aut Sebastian Wachsmann-Hogiu verfasserin aut In Journal of Extracellular Vesicles Wiley, 2012 4(2015), 0, Seite 15 (DE-627)726923710 (DE-600)2683797-3 20013078 nnns volume:4 year:2015 number:0 pages:15 https://doi.org/10.3402/jev.v4.28533 kostenfrei https://doaj.org/article/c8a961dc22c84fe0883483f6f400f53e kostenfrei http://www.journalofextracellularvesicles.net/index.php/jev/article/view/28533/pdf_39 kostenfrei https://doaj.org/toc/2001-3078 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2015 0 15
allfieldsSound	10.3402/jev.v4.28533 doi (DE-627)DOAJ01538215X (DE-599)DOAJc8a961dc22c84fe0883483f6f400f53e DE-627 ger DE-627 rakwb eng QH573-671 Zachary J. Smith verfasserin aut Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Current analysis of exosomes focuses primarily on bulk analysis, where exosome-to-exosome variability cannot be assessed. In this study, we used Raman spectroscopy to study the chemical composition of single exosomes. We measured spectra of individual exosomes from 8 cell lines. Cell-line-averaged spectra varied considerably, reflecting the variation in total exosomal protein, lipid, genetic, and cytosolic content. Unexpectedly, single exosomes isolated from the same cell type also exhibited high spectral variability. Subsequent spectral analysis revealed clustering of single exosomes into 4 distinct groups that were not cell-line specific. Each group contained exosomes from multiple cell lines, and most cell lines had exosomes in multiple groups. The differences between these groups are related to chemical differences primarily due to differing membrane composition. Through a principal components analysis, we identified that the major sources of spectral variation among the exosomes were in cholesterol content, relative expression of phospholipids to cholesterol, and surface protein expression. For example, exosomes derived from cancerous versus non-cancerous cell lines can be largely separated based on their relative expression of cholesterol and phospholipids. We are the first to indicate that exosome subpopulations are shared among cell types, suggesting distributed exosome functionality. The origins of these differences are likely related to the specific role of extracellular vesicle subpopulations in both normal cell function and carcinogenesis, and they may provide diagnostic potential at the single exosome level. exosomes microvesicles single particle Raman laser trap membrane content Cytology Changwon Lee verfasserin aut Tatu Rojalin verfasserin aut Randy P. Carney verfasserin aut Sidhartha Hazari verfasserin aut Alisha Knudson verfasserin aut Kit Lam verfasserin aut Heikki Saari verfasserin aut Elisa Lazaro Ibañez verfasserin aut Tapani Viitala verfasserin aut Timo Laaksonen verfasserin aut Marjo Yliperttula verfasserin aut Sebastian Wachsmann-Hogiu verfasserin aut In Journal of Extracellular Vesicles Wiley, 2012 4(2015), 0, Seite 15 (DE-627)726923710 (DE-600)2683797-3 20013078 nnns volume:4 year:2015 number:0 pages:15 https://doi.org/10.3402/jev.v4.28533 kostenfrei https://doaj.org/article/c8a961dc22c84fe0883483f6f400f53e kostenfrei http://www.journalofextracellularvesicles.net/index.php/jev/article/view/28533/pdf_39 kostenfrei https://doaj.org/toc/2001-3078 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2015 0 15
language	English
source	In Journal of Extracellular Vesicles 4(2015), 0, Seite 15 volume:4 year:2015 number:0 pages:15
sourceStr	In Journal of Extracellular Vesicles 4(2015), 0, Seite 15 volume:4 year:2015 number:0 pages:15
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	exosomes microvesicles single particle Raman laser trap membrane content Cytology
isfreeaccess_bool	true
container_title	Journal of Extracellular Vesicles
authorswithroles_txt_mv	Zachary J. Smith @@aut@@ Changwon Lee @@aut@@ Tatu Rojalin @@aut@@ Randy P. Carney @@aut@@ Sidhartha Hazari @@aut@@ Alisha Knudson @@aut@@ Kit Lam @@aut@@ Heikki Saari @@aut@@ Elisa Lazaro Ibañez @@aut@@ Tapani Viitala @@aut@@ Timo Laaksonen @@aut@@ Marjo Yliperttula @@aut@@ Sebastian Wachsmann-Hogiu @@aut@@
publishDateDaySort_date	2015-01-01T00:00:00Z
hierarchy_top_id	726923710
id	DOAJ01538215X
language_de	englisch
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callnumber-first	Q - Science
author	Zachary J. Smith
spellingShingle	Zachary J. Smith misc QH573-671 misc exosomes misc microvesicles misc single particle misc Raman misc laser trap misc membrane content misc Cytology Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content
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topic_title	QH573-671 Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content exosomes microvesicles single particle Raman laser trap membrane content
topic	misc QH573-671 misc exosomes misc microvesicles misc single particle misc Raman misc laser trap misc membrane content misc Cytology
topic_unstemmed	misc QH573-671 misc exosomes misc microvesicles misc single particle misc Raman misc laser trap misc membrane content misc Cytology
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title	Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content
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title_full	Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content
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author_browse	Zachary J. Smith Changwon Lee Tatu Rojalin Randy P. Carney Sidhartha Hazari Alisha Knudson Kit Lam Heikki Saari Elisa Lazaro Ibañez Tapani Viitala Timo Laaksonen Marjo Yliperttula Sebastian Wachsmann-Hogiu
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title_sort	single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content
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title_auth	Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content
abstract	Current analysis of exosomes focuses primarily on bulk analysis, where exosome-to-exosome variability cannot be assessed. In this study, we used Raman spectroscopy to study the chemical composition of single exosomes. We measured spectra of individual exosomes from 8 cell lines. Cell-line-averaged spectra varied considerably, reflecting the variation in total exosomal protein, lipid, genetic, and cytosolic content. Unexpectedly, single exosomes isolated from the same cell type also exhibited high spectral variability. Subsequent spectral analysis revealed clustering of single exosomes into 4 distinct groups that were not cell-line specific. Each group contained exosomes from multiple cell lines, and most cell lines had exosomes in multiple groups. The differences between these groups are related to chemical differences primarily due to differing membrane composition. Through a principal components analysis, we identified that the major sources of spectral variation among the exosomes were in cholesterol content, relative expression of phospholipids to cholesterol, and surface protein expression. For example, exosomes derived from cancerous versus non-cancerous cell lines can be largely separated based on their relative expression of cholesterol and phospholipids. We are the first to indicate that exosome subpopulations are shared among cell types, suggesting distributed exosome functionality. The origins of these differences are likely related to the specific role of extracellular vesicle subpopulations in both normal cell function and carcinogenesis, and they may provide diagnostic potential at the single exosome level.
abstractGer	Current analysis of exosomes focuses primarily on bulk analysis, where exosome-to-exosome variability cannot be assessed. In this study, we used Raman spectroscopy to study the chemical composition of single exosomes. We measured spectra of individual exosomes from 8 cell lines. Cell-line-averaged spectra varied considerably, reflecting the variation in total exosomal protein, lipid, genetic, and cytosolic content. Unexpectedly, single exosomes isolated from the same cell type also exhibited high spectral variability. Subsequent spectral analysis revealed clustering of single exosomes into 4 distinct groups that were not cell-line specific. Each group contained exosomes from multiple cell lines, and most cell lines had exosomes in multiple groups. The differences between these groups are related to chemical differences primarily due to differing membrane composition. Through a principal components analysis, we identified that the major sources of spectral variation among the exosomes were in cholesterol content, relative expression of phospholipids to cholesterol, and surface protein expression. For example, exosomes derived from cancerous versus non-cancerous cell lines can be largely separated based on their relative expression of cholesterol and phospholipids. We are the first to indicate that exosome subpopulations are shared among cell types, suggesting distributed exosome functionality. The origins of these differences are likely related to the specific role of extracellular vesicle subpopulations in both normal cell function and carcinogenesis, and they may provide diagnostic potential at the single exosome level.
abstract_unstemmed	Current analysis of exosomes focuses primarily on bulk analysis, where exosome-to-exosome variability cannot be assessed. In this study, we used Raman spectroscopy to study the chemical composition of single exosomes. We measured spectra of individual exosomes from 8 cell lines. Cell-line-averaged spectra varied considerably, reflecting the variation in total exosomal protein, lipid, genetic, and cytosolic content. Unexpectedly, single exosomes isolated from the same cell type also exhibited high spectral variability. Subsequent spectral analysis revealed clustering of single exosomes into 4 distinct groups that were not cell-line specific. Each group contained exosomes from multiple cell lines, and most cell lines had exosomes in multiple groups. The differences between these groups are related to chemical differences primarily due to differing membrane composition. Through a principal components analysis, we identified that the major sources of spectral variation among the exosomes were in cholesterol content, relative expression of phospholipids to cholesterol, and surface protein expression. For example, exosomes derived from cancerous versus non-cancerous cell lines can be largely separated based on their relative expression of cholesterol and phospholipids. We are the first to indicate that exosome subpopulations are shared among cell types, suggesting distributed exosome functionality. The origins of these differences are likely related to the specific role of extracellular vesicle subpopulations in both normal cell function and carcinogenesis, and they may provide diagnostic potential at the single exosome level.
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title_short	Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content
url	https://doi.org/10.3402/jev.v4.28533 https://doaj.org/article/c8a961dc22c84fe0883483f6f400f53e http://www.journalofextracellularvesicles.net/index.php/jev/article/view/28533/pdf_39 https://doaj.org/toc/2001-3078
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up_date	2024-07-03T14:40:07.987Z
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fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ01538215X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230310074134.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230226s2015 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3402/jev.v4.28533</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ01538215X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJc8a961dc22c84fe0883483f6f400f53e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH573-671</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Zachary J. Smith</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2015</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Current analysis of exosomes focuses primarily on bulk analysis, where exosome-to-exosome variability cannot be assessed. 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For example, exosomes derived from cancerous versus non-cancerous cell lines can be largely separated based on their relative expression of cholesterol and phospholipids. We are the first to indicate that exosome subpopulations are shared among cell types, suggesting distributed exosome functionality. 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Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content

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