A Visual Discrimination of Existing States of Virus Capsid Protein by a Giant Molybdate Cluster

We report a unique phenomenon, the opposite color response of a giant polyoxometalate, (NH<sub<4</sub<)<sub<42</sub<[Mo<sub<132</sub<O<sub<372</sub<(CHCOO)<sub<30</sub<] (H<sub<2</sub<O)<sub<72</sub< ([Mo<sub<1...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Yarong Xue [verfasserIn] Mingfen Wei [verfasserIn] Dingyi Fu [verfasserIn] Yuqing Wu [verfasserIn] Bo Sun [verfasserIn] Xianghui Yu [verfasserIn] Lixin Wu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	colorimetric discrimination giant polyoxometalate HPV capsid protein hypochromic of molybdate clusters opposite color response

Übergeordnetes Werk:	In: Nanomaterials - MDPI AG, 2012, 12(2022), 5, p 736
Übergeordnetes Werk:	volume:12 ; year:2022 ; number:5, p 736

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/nano12050736

Katalog-ID:	DOAJ015390489

Internformat


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520			\|a We report a unique phenomenon, the opposite color response of a giant polyoxometalate, (NH<sub<4</sub<)<sub<42</sub<[Mo<sub<132</sub<O<sub<372</sub<(CHCOO)<sub<30</sub<] (H<sub<2</sub<O)<sub<72</sub< ([Mo<sub<132</sub<]), to the existing states of human papillomavirus (HPV) major capsid protein, L1-pentamer (L1-p), and virus-like particles (VLPs). The color responses originate from the different assembly forms between [Mo<sub<132</sub<] and the capsid protein. The latter were inspected and separated by using CsCl gradient centrifugation, and validated in detail by sodium dodecyl sulfate-polyacrylamide gel-electrophoresis (SDS-PAGE), dynamic light scattering (DLS), and transmission electron microscopy (TEM) imaging. Furthermore, the intrinsic mechanisms were investigated in-depth by using XPS-based semi-quantitative analysis and well-designed peptides, revealing the critical points of L1 that determine the charge–transfer ratio between Mo(V) to Mo(VI), and consequently, the levels of [Mo<sub<132</sub<] hypochromic in different assemblies. Such a unique phenomenon is significant as it supplies a colorimetry approach to distinguish the existing states of the HPV capsid protein and would be significant in the quality assay of the HPV vaccine and existing states of other viruses in the future.
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allfields	10.3390/nano12050736 doi (DE-627)DOAJ015390489 (DE-599)DOAJd80280f5b22140f582425fea8148f3f9 DE-627 ger DE-627 rakwb eng QD1-999 Yarong Xue verfasserin aut A Visual Discrimination of Existing States of Virus Capsid Protein by a Giant Molybdate Cluster 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We report a unique phenomenon, the opposite color response of a giant polyoxometalate, (NH<sub<4</sub<)<sub<42</sub<[Mo<sub<132</sub<O<sub<372</sub<(CHCOO)<sub<30</sub<] (H<sub<2</sub<O)<sub<72</sub< ([Mo<sub<132</sub<]), to the existing states of human papillomavirus (HPV) major capsid protein, L1-pentamer (L1-p), and virus-like particles (VLPs). The color responses originate from the different assembly forms between [Mo<sub<132</sub<] and the capsid protein. The latter were inspected and separated by using CsCl gradient centrifugation, and validated in detail by sodium dodecyl sulfate-polyacrylamide gel-electrophoresis (SDS-PAGE), dynamic light scattering (DLS), and transmission electron microscopy (TEM) imaging. Furthermore, the intrinsic mechanisms were investigated in-depth by using XPS-based semi-quantitative analysis and well-designed peptides, revealing the critical points of L1 that determine the charge–transfer ratio between Mo(V) to Mo(VI), and consequently, the levels of [Mo<sub<132</sub<] hypochromic in different assemblies. Such a unique phenomenon is significant as it supplies a colorimetry approach to distinguish the existing states of the HPV capsid protein and would be significant in the quality assay of the HPV vaccine and existing states of other viruses in the future. colorimetric discrimination giant polyoxometalate HPV capsid protein hypochromic of molybdate clusters opposite color response Chemistry Mingfen Wei verfasserin aut Dingyi Fu verfasserin aut Yuqing Wu verfasserin aut Bo Sun verfasserin aut Xianghui Yu verfasserin aut Lixin Wu verfasserin aut In Nanomaterials MDPI AG, 2012 12(2022), 5, p 736 (DE-627)718627199 (DE-600)2662255-5 20794991 nnns volume:12 year:2022 number:5, p 736 https://doi.org/10.3390/nano12050736 kostenfrei https://doaj.org/article/d80280f5b22140f582425fea8148f3f9 kostenfrei https://www.mdpi.com/2079-4991/12/5/736 kostenfrei https://doaj.org/toc/2079-4991 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 5, p 736
spelling	10.3390/nano12050736 doi (DE-627)DOAJ015390489 (DE-599)DOAJd80280f5b22140f582425fea8148f3f9 DE-627 ger DE-627 rakwb eng QD1-999 Yarong Xue verfasserin aut A Visual Discrimination of Existing States of Virus Capsid Protein by a Giant Molybdate Cluster 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We report a unique phenomenon, the opposite color response of a giant polyoxometalate, (NH<sub<4</sub<)<sub<42</sub<[Mo<sub<132</sub<O<sub<372</sub<(CHCOO)<sub<30</sub<] (H<sub<2</sub<O)<sub<72</sub< ([Mo<sub<132</sub<]), to the existing states of human papillomavirus (HPV) major capsid protein, L1-pentamer (L1-p), and virus-like particles (VLPs). The color responses originate from the different assembly forms between [Mo<sub<132</sub<] and the capsid protein. The latter were inspected and separated by using CsCl gradient centrifugation, and validated in detail by sodium dodecyl sulfate-polyacrylamide gel-electrophoresis (SDS-PAGE), dynamic light scattering (DLS), and transmission electron microscopy (TEM) imaging. Furthermore, the intrinsic mechanisms were investigated in-depth by using XPS-based semi-quantitative analysis and well-designed peptides, revealing the critical points of L1 that determine the charge–transfer ratio between Mo(V) to Mo(VI), and consequently, the levels of [Mo<sub<132</sub<] hypochromic in different assemblies. Such a unique phenomenon is significant as it supplies a colorimetry approach to distinguish the existing states of the HPV capsid protein and would be significant in the quality assay of the HPV vaccine and existing states of other viruses in the future. colorimetric discrimination giant polyoxometalate HPV capsid protein hypochromic of molybdate clusters opposite color response Chemistry Mingfen Wei verfasserin aut Dingyi Fu verfasserin aut Yuqing Wu verfasserin aut Bo Sun verfasserin aut Xianghui Yu verfasserin aut Lixin Wu verfasserin aut In Nanomaterials MDPI AG, 2012 12(2022), 5, p 736 (DE-627)718627199 (DE-600)2662255-5 20794991 nnns volume:12 year:2022 number:5, p 736 https://doi.org/10.3390/nano12050736 kostenfrei https://doaj.org/article/d80280f5b22140f582425fea8148f3f9 kostenfrei https://www.mdpi.com/2079-4991/12/5/736 kostenfrei https://doaj.org/toc/2079-4991 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 5, p 736
allfields_unstemmed	10.3390/nano12050736 doi (DE-627)DOAJ015390489 (DE-599)DOAJd80280f5b22140f582425fea8148f3f9 DE-627 ger DE-627 rakwb eng QD1-999 Yarong Xue verfasserin aut A Visual Discrimination of Existing States of Virus Capsid Protein by a Giant Molybdate Cluster 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We report a unique phenomenon, the opposite color response of a giant polyoxometalate, (NH<sub<4</sub<)<sub<42</sub<[Mo<sub<132</sub<O<sub<372</sub<(CHCOO)<sub<30</sub<] (H<sub<2</sub<O)<sub<72</sub< ([Mo<sub<132</sub<]), to the existing states of human papillomavirus (HPV) major capsid protein, L1-pentamer (L1-p), and virus-like particles (VLPs). The color responses originate from the different assembly forms between [Mo<sub<132</sub<] and the capsid protein. The latter were inspected and separated by using CsCl gradient centrifugation, and validated in detail by sodium dodecyl sulfate-polyacrylamide gel-electrophoresis (SDS-PAGE), dynamic light scattering (DLS), and transmission electron microscopy (TEM) imaging. Furthermore, the intrinsic mechanisms were investigated in-depth by using XPS-based semi-quantitative analysis and well-designed peptides, revealing the critical points of L1 that determine the charge–transfer ratio between Mo(V) to Mo(VI), and consequently, the levels of [Mo<sub<132</sub<] hypochromic in different assemblies. Such a unique phenomenon is significant as it supplies a colorimetry approach to distinguish the existing states of the HPV capsid protein and would be significant in the quality assay of the HPV vaccine and existing states of other viruses in the future. colorimetric discrimination giant polyoxometalate HPV capsid protein hypochromic of molybdate clusters opposite color response Chemistry Mingfen Wei verfasserin aut Dingyi Fu verfasserin aut Yuqing Wu verfasserin aut Bo Sun verfasserin aut Xianghui Yu verfasserin aut Lixin Wu verfasserin aut In Nanomaterials MDPI AG, 2012 12(2022), 5, p 736 (DE-627)718627199 (DE-600)2662255-5 20794991 nnns volume:12 year:2022 number:5, p 736 https://doi.org/10.3390/nano12050736 kostenfrei https://doaj.org/article/d80280f5b22140f582425fea8148f3f9 kostenfrei https://www.mdpi.com/2079-4991/12/5/736 kostenfrei https://doaj.org/toc/2079-4991 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 5, p 736
allfieldsGer	10.3390/nano12050736 doi (DE-627)DOAJ015390489 (DE-599)DOAJd80280f5b22140f582425fea8148f3f9 DE-627 ger DE-627 rakwb eng QD1-999 Yarong Xue verfasserin aut A Visual Discrimination of Existing States of Virus Capsid Protein by a Giant Molybdate Cluster 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We report a unique phenomenon, the opposite color response of a giant polyoxometalate, (NH<sub<4</sub<)<sub<42</sub<[Mo<sub<132</sub<O<sub<372</sub<(CHCOO)<sub<30</sub<] (H<sub<2</sub<O)<sub<72</sub< ([Mo<sub<132</sub<]), to the existing states of human papillomavirus (HPV) major capsid protein, L1-pentamer (L1-p), and virus-like particles (VLPs). The color responses originate from the different assembly forms between [Mo<sub<132</sub<] and the capsid protein. The latter were inspected and separated by using CsCl gradient centrifugation, and validated in detail by sodium dodecyl sulfate-polyacrylamide gel-electrophoresis (SDS-PAGE), dynamic light scattering (DLS), and transmission electron microscopy (TEM) imaging. Furthermore, the intrinsic mechanisms were investigated in-depth by using XPS-based semi-quantitative analysis and well-designed peptides, revealing the critical points of L1 that determine the charge–transfer ratio between Mo(V) to Mo(VI), and consequently, the levels of [Mo<sub<132</sub<] hypochromic in different assemblies. Such a unique phenomenon is significant as it supplies a colorimetry approach to distinguish the existing states of the HPV capsid protein and would be significant in the quality assay of the HPV vaccine and existing states of other viruses in the future. colorimetric discrimination giant polyoxometalate HPV capsid protein hypochromic of molybdate clusters opposite color response Chemistry Mingfen Wei verfasserin aut Dingyi Fu verfasserin aut Yuqing Wu verfasserin aut Bo Sun verfasserin aut Xianghui Yu verfasserin aut Lixin Wu verfasserin aut In Nanomaterials MDPI AG, 2012 12(2022), 5, p 736 (DE-627)718627199 (DE-600)2662255-5 20794991 nnns volume:12 year:2022 number:5, p 736 https://doi.org/10.3390/nano12050736 kostenfrei https://doaj.org/article/d80280f5b22140f582425fea8148f3f9 kostenfrei https://www.mdpi.com/2079-4991/12/5/736 kostenfrei https://doaj.org/toc/2079-4991 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 5, p 736
allfieldsSound	10.3390/nano12050736 doi (DE-627)DOAJ015390489 (DE-599)DOAJd80280f5b22140f582425fea8148f3f9 DE-627 ger DE-627 rakwb eng QD1-999 Yarong Xue verfasserin aut A Visual Discrimination of Existing States of Virus Capsid Protein by a Giant Molybdate Cluster 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We report a unique phenomenon, the opposite color response of a giant polyoxometalate, (NH<sub<4</sub<)<sub<42</sub<[Mo<sub<132</sub<O<sub<372</sub<(CHCOO)<sub<30</sub<] (H<sub<2</sub<O)<sub<72</sub< ([Mo<sub<132</sub<]), to the existing states of human papillomavirus (HPV) major capsid protein, L1-pentamer (L1-p), and virus-like particles (VLPs). The color responses originate from the different assembly forms between [Mo<sub<132</sub<] and the capsid protein. The latter were inspected and separated by using CsCl gradient centrifugation, and validated in detail by sodium dodecyl sulfate-polyacrylamide gel-electrophoresis (SDS-PAGE), dynamic light scattering (DLS), and transmission electron microscopy (TEM) imaging. Furthermore, the intrinsic mechanisms were investigated in-depth by using XPS-based semi-quantitative analysis and well-designed peptides, revealing the critical points of L1 that determine the charge–transfer ratio between Mo(V) to Mo(VI), and consequently, the levels of [Mo<sub<132</sub<] hypochromic in different assemblies. Such a unique phenomenon is significant as it supplies a colorimetry approach to distinguish the existing states of the HPV capsid protein and would be significant in the quality assay of the HPV vaccine and existing states of other viruses in the future. colorimetric discrimination giant polyoxometalate HPV capsid protein hypochromic of molybdate clusters opposite color response Chemistry Mingfen Wei verfasserin aut Dingyi Fu verfasserin aut Yuqing Wu verfasserin aut Bo Sun verfasserin aut Xianghui Yu verfasserin aut Lixin Wu verfasserin aut In Nanomaterials MDPI AG, 2012 12(2022), 5, p 736 (DE-627)718627199 (DE-600)2662255-5 20794991 nnns volume:12 year:2022 number:5, p 736 https://doi.org/10.3390/nano12050736 kostenfrei https://doaj.org/article/d80280f5b22140f582425fea8148f3f9 kostenfrei https://www.mdpi.com/2079-4991/12/5/736 kostenfrei https://doaj.org/toc/2079-4991 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 5, p 736
language	English
source	In Nanomaterials 12(2022), 5, p 736 volume:12 year:2022 number:5, p 736
sourceStr	In Nanomaterials 12(2022), 5, p 736 volume:12 year:2022 number:5, p 736
format_phy_str_mv	Article
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topic_facet	colorimetric discrimination giant polyoxometalate HPV capsid protein hypochromic of molybdate clusters opposite color response Chemistry
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authorswithroles_txt_mv	Yarong Xue @@aut@@ Mingfen Wei @@aut@@ Dingyi Fu @@aut@@ Yuqing Wu @@aut@@ Bo Sun @@aut@@ Xianghui Yu @@aut@@ Lixin Wu @@aut@@
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callnumber-first	Q - Science
author	Yarong Xue
spellingShingle	Yarong Xue misc QD1-999 misc colorimetric discrimination misc giant polyoxometalate misc HPV capsid protein misc hypochromic of molybdate clusters misc opposite color response misc Chemistry A Visual Discrimination of Existing States of Virus Capsid Protein by a Giant Molybdate Cluster
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topic_title	QD1-999 A Visual Discrimination of Existing States of Virus Capsid Protein by a Giant Molybdate Cluster colorimetric discrimination giant polyoxometalate HPV capsid protein hypochromic of molybdate clusters opposite color response
topic	misc QD1-999 misc colorimetric discrimination misc giant polyoxometalate misc HPV capsid protein misc hypochromic of molybdate clusters misc opposite color response misc Chemistry
topic_unstemmed	misc QD1-999 misc colorimetric discrimination misc giant polyoxometalate misc HPV capsid protein misc hypochromic of molybdate clusters misc opposite color response misc Chemistry
topic_browse	misc QD1-999 misc colorimetric discrimination misc giant polyoxometalate misc HPV capsid protein misc hypochromic of molybdate clusters misc opposite color response misc Chemistry
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title	A Visual Discrimination of Existing States of Virus Capsid Protein by a Giant Molybdate Cluster
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title_full	A Visual Discrimination of Existing States of Virus Capsid Protein by a Giant Molybdate Cluster
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author_browse	Yarong Xue Mingfen Wei Dingyi Fu Yuqing Wu Bo Sun Xianghui Yu Lixin Wu
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title_sort	visual discrimination of existing states of virus capsid protein by a giant molybdate cluster
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title_auth	A Visual Discrimination of Existing States of Virus Capsid Protein by a Giant Molybdate Cluster
abstract	We report a unique phenomenon, the opposite color response of a giant polyoxometalate, (NH<sub<4</sub<)<sub<42</sub<[Mo<sub<132</sub<O<sub<372</sub<(CHCOO)<sub<30</sub<] (H<sub<2</sub<O)<sub<72</sub< ([Mo<sub<132</sub<]), to the existing states of human papillomavirus (HPV) major capsid protein, L1-pentamer (L1-p), and virus-like particles (VLPs). The color responses originate from the different assembly forms between [Mo<sub<132</sub<] and the capsid protein. The latter were inspected and separated by using CsCl gradient centrifugation, and validated in detail by sodium dodecyl sulfate-polyacrylamide gel-electrophoresis (SDS-PAGE), dynamic light scattering (DLS), and transmission electron microscopy (TEM) imaging. Furthermore, the intrinsic mechanisms were investigated in-depth by using XPS-based semi-quantitative analysis and well-designed peptides, revealing the critical points of L1 that determine the charge–transfer ratio between Mo(V) to Mo(VI), and consequently, the levels of [Mo<sub<132</sub<] hypochromic in different assemblies. Such a unique phenomenon is significant as it supplies a colorimetry approach to distinguish the existing states of the HPV capsid protein and would be significant in the quality assay of the HPV vaccine and existing states of other viruses in the future.
abstractGer	We report a unique phenomenon, the opposite color response of a giant polyoxometalate, (NH<sub<4</sub<)<sub<42</sub<[Mo<sub<132</sub<O<sub<372</sub<(CHCOO)<sub<30</sub<] (H<sub<2</sub<O)<sub<72</sub< ([Mo<sub<132</sub<]), to the existing states of human papillomavirus (HPV) major capsid protein, L1-pentamer (L1-p), and virus-like particles (VLPs). The color responses originate from the different assembly forms between [Mo<sub<132</sub<] and the capsid protein. The latter were inspected and separated by using CsCl gradient centrifugation, and validated in detail by sodium dodecyl sulfate-polyacrylamide gel-electrophoresis (SDS-PAGE), dynamic light scattering (DLS), and transmission electron microscopy (TEM) imaging. Furthermore, the intrinsic mechanisms were investigated in-depth by using XPS-based semi-quantitative analysis and well-designed peptides, revealing the critical points of L1 that determine the charge–transfer ratio between Mo(V) to Mo(VI), and consequently, the levels of [Mo<sub<132</sub<] hypochromic in different assemblies. Such a unique phenomenon is significant as it supplies a colorimetry approach to distinguish the existing states of the HPV capsid protein and would be significant in the quality assay of the HPV vaccine and existing states of other viruses in the future.
abstract_unstemmed	We report a unique phenomenon, the opposite color response of a giant polyoxometalate, (NH<sub<4</sub<)<sub<42</sub<[Mo<sub<132</sub<O<sub<372</sub<(CHCOO)<sub<30</sub<] (H<sub<2</sub<O)<sub<72</sub< ([Mo<sub<132</sub<]), to the existing states of human papillomavirus (HPV) major capsid protein, L1-pentamer (L1-p), and virus-like particles (VLPs). The color responses originate from the different assembly forms between [Mo<sub<132</sub<] and the capsid protein. The latter were inspected and separated by using CsCl gradient centrifugation, and validated in detail by sodium dodecyl sulfate-polyacrylamide gel-electrophoresis (SDS-PAGE), dynamic light scattering (DLS), and transmission electron microscopy (TEM) imaging. Furthermore, the intrinsic mechanisms were investigated in-depth by using XPS-based semi-quantitative analysis and well-designed peptides, revealing the critical points of L1 that determine the charge–transfer ratio between Mo(V) to Mo(VI), and consequently, the levels of [Mo<sub<132</sub<] hypochromic in different assemblies. Such a unique phenomenon is significant as it supplies a colorimetry approach to distinguish the existing states of the HPV capsid protein and would be significant in the quality assay of the HPV vaccine and existing states of other viruses in the future.
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title_short	A Visual Discrimination of Existing States of Virus Capsid Protein by a Giant Molybdate Cluster
url	https://doi.org/10.3390/nano12050736 https://doaj.org/article/d80280f5b22140f582425fea8148f3f9 https://www.mdpi.com/2079-4991/12/5/736 https://doaj.org/toc/2079-4991
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up_date	2024-07-03T14:43:28.670Z
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A Visual Discrimination of Existing States of Virus Capsid Protein by a Giant Molybdate Cluster

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