Genetic diversity & drug sensitivity profiles of Mycobacterium tuberculosis isolates from two slums of Jaipur city, Rajasthan, India
Background & objectives: Slums are considered as hotspots of tuberculosis (TB). The study of genetic diversity and drug susceptibility profile of Mycobacterium tuberculosis (MTB) will help understand the transmission dynamics and can be used for better prevention and control of the disease. The...
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| Autor*in: |
Bharti Malhotra [verfasserIn] Deepti Dashora [verfasserIn] Vipin Kumar [verfasserIn] Sumit Goyal [verfasserIn] Bhavana Sharma [verfasserIn] Madhu Kumar [verfasserIn] Kailash Narayan Gupta [verfasserIn] Vishnu Dutt Sharma [verfasserIn] D S Chauhan [verfasserIn] Kiran Katoch [verfasserIn] Vishwa Mohan Katoch [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2017 |
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| Übergeordnetes Werk: |
In: Indian Journal of Medical Research - Wolters Kluwer Medknow Publications, 2005, 145(2017), 1, Seite 74-83 |
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| Übergeordnetes Werk: |
volume:145 ; year:2017 ; number:1 ; pages:74-83 |
| Links: |
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| DOI / URN: |
10.4103/ijmr.IJMR_336_14 |
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| Katalog-ID: |
DOAJ015846814 |
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| 520 | |a Background & objectives: Slums are considered as hotspots of tuberculosis (TB). The study of genetic diversity and drug susceptibility profile of Mycobacterium tuberculosis (MTB) will help understand the transmission dynamics and can be used for better prevention and control of the disease. The aim of this study was to determine the drug susceptibility profiles and genetic diversity using the random amplified polymorphic DNA (RAPD) and mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU VNTR) of MTB isolates from sputum samples of pulmonary TB patients residing in the two slums of Jaipur city in Rajasthan, India. Methods: Sputum samples collected from pulmonary TB patients, their contacts and suspects during 2010-2012 were processed for microscopy and mycobacterial culture. Drug susceptibility testing was done by one per cent indirect proportion method on Lowenstein–Jensen medium for first-line anti-TB drugs rifampicin, isoniazid, ethambutol and streptomycin. MTB DNA was extracted by physicochemical method, and DNA fingerprinting was done by RAPD and MIRU VNTR analysis. Results: Among 175 sputum samples collected, 75 were positive (43.8%) for acid-fast bacilli, 83 for MTB culture and four were contaminated. Fifty two isolates (62.7%) were fully sensitive to four drugs, and five (6%) were multidrug resistant (MDR). RAPD analysis of 81 isolates revealed six clusters containing 23 (28.4%) isolates, and 58 (71.6%) were unique. MIRU VNTR analysis clustered 20 (24.7%) isolates, and 61 (75.3%) were unique. Interpretation & conclusions: About 62.7 per cent isolates from the sputum samples from slum areas were sensitive to four drugs; six per cent of isolates were MDR. Poly-resistance other than MDR was high (16%). About one-fourth isolates were clustered by either method. RAPD was rapid, less expensive but had low reproducibility. MIRU VNTR analysis could identify to greater extent the epidemiological link in the population studied. | ||
| 650 | 4 | |a Drug susceptibility testing - mycobacterial interspersed repetitive units-variable number of tandem repeats - Mycobacterium tuberculosis - random amplified polymorphic DNA | |
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10.4103/ijmr.IJMR_336_14 doi (DE-627)DOAJ015846814 (DE-599)DOAJa8f99e22da134031b1fa50a51609a241 DE-627 ger DE-627 rakwb eng Bharti Malhotra verfasserin aut Genetic diversity & drug sensitivity profiles of Mycobacterium tuberculosis isolates from two slums of Jaipur city, Rajasthan, India 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background & objectives: Slums are considered as hotspots of tuberculosis (TB). The study of genetic diversity and drug susceptibility profile of Mycobacterium tuberculosis (MTB) will help understand the transmission dynamics and can be used for better prevention and control of the disease. The aim of this study was to determine the drug susceptibility profiles and genetic diversity using the random amplified polymorphic DNA (RAPD) and mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU VNTR) of MTB isolates from sputum samples of pulmonary TB patients residing in the two slums of Jaipur city in Rajasthan, India. Methods: Sputum samples collected from pulmonary TB patients, their contacts and suspects during 2010-2012 were processed for microscopy and mycobacterial culture. Drug susceptibility testing was done by one per cent indirect proportion method on Lowenstein–Jensen medium for first-line anti-TB drugs rifampicin, isoniazid, ethambutol and streptomycin. MTB DNA was extracted by physicochemical method, and DNA fingerprinting was done by RAPD and MIRU VNTR analysis. Results: Among 175 sputum samples collected, 75 were positive (43.8%) for acid-fast bacilli, 83 for MTB culture and four were contaminated. Fifty two isolates (62.7%) were fully sensitive to four drugs, and five (6%) were multidrug resistant (MDR). RAPD analysis of 81 isolates revealed six clusters containing 23 (28.4%) isolates, and 58 (71.6%) were unique. MIRU VNTR analysis clustered 20 (24.7%) isolates, and 61 (75.3%) were unique. Interpretation & conclusions: About 62.7 per cent isolates from the sputum samples from slum areas were sensitive to four drugs; six per cent of isolates were MDR. Poly-resistance other than MDR was high (16%). About one-fourth isolates were clustered by either method. RAPD was rapid, less expensive but had low reproducibility. MIRU VNTR analysis could identify to greater extent the epidemiological link in the population studied. Drug susceptibility testing - mycobacterial interspersed repetitive units-variable number of tandem repeats - Mycobacterium tuberculosis - random amplified polymorphic DNA Medicine R Deepti Dashora verfasserin aut Vipin Kumar verfasserin aut Sumit Goyal verfasserin aut Bhavana Sharma verfasserin aut Madhu Kumar verfasserin aut Kailash Narayan Gupta verfasserin aut Vishnu Dutt Sharma verfasserin aut D S Chauhan verfasserin aut Kiran Katoch verfasserin aut Vishwa Mohan Katoch verfasserin aut In Indian Journal of Medical Research Wolters Kluwer Medknow Publications, 2005 145(2017), 1, Seite 74-83 (DE-627)DOAJ000026956 09715916 nnns volume:145 year:2017 number:1 pages:74-83 https://doi.org/10.4103/ijmr.IJMR_336_14 kostenfrei https://doaj.org/article/a8f99e22da134031b1fa50a51609a241 kostenfrei http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2017;volume=145;issue=1;spage=74;epage=83;aulast=Malhotra kostenfrei https://doaj.org/toc/0971-5916 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 145 2017 1 74-83 |
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10.4103/ijmr.IJMR_336_14 doi (DE-627)DOAJ015846814 (DE-599)DOAJa8f99e22da134031b1fa50a51609a241 DE-627 ger DE-627 rakwb eng Bharti Malhotra verfasserin aut Genetic diversity & drug sensitivity profiles of Mycobacterium tuberculosis isolates from two slums of Jaipur city, Rajasthan, India 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background & objectives: Slums are considered as hotspots of tuberculosis (TB). The study of genetic diversity and drug susceptibility profile of Mycobacterium tuberculosis (MTB) will help understand the transmission dynamics and can be used for better prevention and control of the disease. The aim of this study was to determine the drug susceptibility profiles and genetic diversity using the random amplified polymorphic DNA (RAPD) and mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU VNTR) of MTB isolates from sputum samples of pulmonary TB patients residing in the two slums of Jaipur city in Rajasthan, India. Methods: Sputum samples collected from pulmonary TB patients, their contacts and suspects during 2010-2012 were processed for microscopy and mycobacterial culture. Drug susceptibility testing was done by one per cent indirect proportion method on Lowenstein–Jensen medium for first-line anti-TB drugs rifampicin, isoniazid, ethambutol and streptomycin. MTB DNA was extracted by physicochemical method, and DNA fingerprinting was done by RAPD and MIRU VNTR analysis. Results: Among 175 sputum samples collected, 75 were positive (43.8%) for acid-fast bacilli, 83 for MTB culture and four were contaminated. Fifty two isolates (62.7%) were fully sensitive to four drugs, and five (6%) were multidrug resistant (MDR). RAPD analysis of 81 isolates revealed six clusters containing 23 (28.4%) isolates, and 58 (71.6%) were unique. MIRU VNTR analysis clustered 20 (24.7%) isolates, and 61 (75.3%) were unique. Interpretation & conclusions: About 62.7 per cent isolates from the sputum samples from slum areas were sensitive to four drugs; six per cent of isolates were MDR. Poly-resistance other than MDR was high (16%). About one-fourth isolates were clustered by either method. RAPD was rapid, less expensive but had low reproducibility. MIRU VNTR analysis could identify to greater extent the epidemiological link in the population studied. Drug susceptibility testing - mycobacterial interspersed repetitive units-variable number of tandem repeats - Mycobacterium tuberculosis - random amplified polymorphic DNA Medicine R Deepti Dashora verfasserin aut Vipin Kumar verfasserin aut Sumit Goyal verfasserin aut Bhavana Sharma verfasserin aut Madhu Kumar verfasserin aut Kailash Narayan Gupta verfasserin aut Vishnu Dutt Sharma verfasserin aut D S Chauhan verfasserin aut Kiran Katoch verfasserin aut Vishwa Mohan Katoch verfasserin aut In Indian Journal of Medical Research Wolters Kluwer Medknow Publications, 2005 145(2017), 1, Seite 74-83 (DE-627)DOAJ000026956 09715916 nnns volume:145 year:2017 number:1 pages:74-83 https://doi.org/10.4103/ijmr.IJMR_336_14 kostenfrei https://doaj.org/article/a8f99e22da134031b1fa50a51609a241 kostenfrei http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2017;volume=145;issue=1;spage=74;epage=83;aulast=Malhotra kostenfrei https://doaj.org/toc/0971-5916 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 145 2017 1 74-83 |
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10.4103/ijmr.IJMR_336_14 doi (DE-627)DOAJ015846814 (DE-599)DOAJa8f99e22da134031b1fa50a51609a241 DE-627 ger DE-627 rakwb eng Bharti Malhotra verfasserin aut Genetic diversity & drug sensitivity profiles of Mycobacterium tuberculosis isolates from two slums of Jaipur city, Rajasthan, India 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background & objectives: Slums are considered as hotspots of tuberculosis (TB). The study of genetic diversity and drug susceptibility profile of Mycobacterium tuberculosis (MTB) will help understand the transmission dynamics and can be used for better prevention and control of the disease. The aim of this study was to determine the drug susceptibility profiles and genetic diversity using the random amplified polymorphic DNA (RAPD) and mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU VNTR) of MTB isolates from sputum samples of pulmonary TB patients residing in the two slums of Jaipur city in Rajasthan, India. Methods: Sputum samples collected from pulmonary TB patients, their contacts and suspects during 2010-2012 were processed for microscopy and mycobacterial culture. Drug susceptibility testing was done by one per cent indirect proportion method on Lowenstein–Jensen medium for first-line anti-TB drugs rifampicin, isoniazid, ethambutol and streptomycin. MTB DNA was extracted by physicochemical method, and DNA fingerprinting was done by RAPD and MIRU VNTR analysis. Results: Among 175 sputum samples collected, 75 were positive (43.8%) for acid-fast bacilli, 83 for MTB culture and four were contaminated. Fifty two isolates (62.7%) were fully sensitive to four drugs, and five (6%) were multidrug resistant (MDR). RAPD analysis of 81 isolates revealed six clusters containing 23 (28.4%) isolates, and 58 (71.6%) were unique. MIRU VNTR analysis clustered 20 (24.7%) isolates, and 61 (75.3%) were unique. Interpretation & conclusions: About 62.7 per cent isolates from the sputum samples from slum areas were sensitive to four drugs; six per cent of isolates were MDR. Poly-resistance other than MDR was high (16%). About one-fourth isolates were clustered by either method. RAPD was rapid, less expensive but had low reproducibility. MIRU VNTR analysis could identify to greater extent the epidemiological link in the population studied. Drug susceptibility testing - mycobacterial interspersed repetitive units-variable number of tandem repeats - Mycobacterium tuberculosis - random amplified polymorphic DNA Medicine R Deepti Dashora verfasserin aut Vipin Kumar verfasserin aut Sumit Goyal verfasserin aut Bhavana Sharma verfasserin aut Madhu Kumar verfasserin aut Kailash Narayan Gupta verfasserin aut Vishnu Dutt Sharma verfasserin aut D S Chauhan verfasserin aut Kiran Katoch verfasserin aut Vishwa Mohan Katoch verfasserin aut In Indian Journal of Medical Research Wolters Kluwer Medknow Publications, 2005 145(2017), 1, Seite 74-83 (DE-627)DOAJ000026956 09715916 nnns volume:145 year:2017 number:1 pages:74-83 https://doi.org/10.4103/ijmr.IJMR_336_14 kostenfrei https://doaj.org/article/a8f99e22da134031b1fa50a51609a241 kostenfrei http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2017;volume=145;issue=1;spage=74;epage=83;aulast=Malhotra kostenfrei https://doaj.org/toc/0971-5916 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 145 2017 1 74-83 |
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10.4103/ijmr.IJMR_336_14 doi (DE-627)DOAJ015846814 (DE-599)DOAJa8f99e22da134031b1fa50a51609a241 DE-627 ger DE-627 rakwb eng Bharti Malhotra verfasserin aut Genetic diversity & drug sensitivity profiles of Mycobacterium tuberculosis isolates from two slums of Jaipur city, Rajasthan, India 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background & objectives: Slums are considered as hotspots of tuberculosis (TB). The study of genetic diversity and drug susceptibility profile of Mycobacterium tuberculosis (MTB) will help understand the transmission dynamics and can be used for better prevention and control of the disease. The aim of this study was to determine the drug susceptibility profiles and genetic diversity using the random amplified polymorphic DNA (RAPD) and mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU VNTR) of MTB isolates from sputum samples of pulmonary TB patients residing in the two slums of Jaipur city in Rajasthan, India. Methods: Sputum samples collected from pulmonary TB patients, their contacts and suspects during 2010-2012 were processed for microscopy and mycobacterial culture. Drug susceptibility testing was done by one per cent indirect proportion method on Lowenstein–Jensen medium for first-line anti-TB drugs rifampicin, isoniazid, ethambutol and streptomycin. MTB DNA was extracted by physicochemical method, and DNA fingerprinting was done by RAPD and MIRU VNTR analysis. Results: Among 175 sputum samples collected, 75 were positive (43.8%) for acid-fast bacilli, 83 for MTB culture and four were contaminated. Fifty two isolates (62.7%) were fully sensitive to four drugs, and five (6%) were multidrug resistant (MDR). RAPD analysis of 81 isolates revealed six clusters containing 23 (28.4%) isolates, and 58 (71.6%) were unique. MIRU VNTR analysis clustered 20 (24.7%) isolates, and 61 (75.3%) were unique. Interpretation & conclusions: About 62.7 per cent isolates from the sputum samples from slum areas were sensitive to four drugs; six per cent of isolates were MDR. Poly-resistance other than MDR was high (16%). About one-fourth isolates were clustered by either method. RAPD was rapid, less expensive but had low reproducibility. MIRU VNTR analysis could identify to greater extent the epidemiological link in the population studied. Drug susceptibility testing - mycobacterial interspersed repetitive units-variable number of tandem repeats - Mycobacterium tuberculosis - random amplified polymorphic DNA Medicine R Deepti Dashora verfasserin aut Vipin Kumar verfasserin aut Sumit Goyal verfasserin aut Bhavana Sharma verfasserin aut Madhu Kumar verfasserin aut Kailash Narayan Gupta verfasserin aut Vishnu Dutt Sharma verfasserin aut D S Chauhan verfasserin aut Kiran Katoch verfasserin aut Vishwa Mohan Katoch verfasserin aut In Indian Journal of Medical Research Wolters Kluwer Medknow Publications, 2005 145(2017), 1, Seite 74-83 (DE-627)DOAJ000026956 09715916 nnns volume:145 year:2017 number:1 pages:74-83 https://doi.org/10.4103/ijmr.IJMR_336_14 kostenfrei https://doaj.org/article/a8f99e22da134031b1fa50a51609a241 kostenfrei http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2017;volume=145;issue=1;spage=74;epage=83;aulast=Malhotra kostenfrei https://doaj.org/toc/0971-5916 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 145 2017 1 74-83 |
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10.4103/ijmr.IJMR_336_14 doi (DE-627)DOAJ015846814 (DE-599)DOAJa8f99e22da134031b1fa50a51609a241 DE-627 ger DE-627 rakwb eng Bharti Malhotra verfasserin aut Genetic diversity & drug sensitivity profiles of Mycobacterium tuberculosis isolates from two slums of Jaipur city, Rajasthan, India 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background & objectives: Slums are considered as hotspots of tuberculosis (TB). The study of genetic diversity and drug susceptibility profile of Mycobacterium tuberculosis (MTB) will help understand the transmission dynamics and can be used for better prevention and control of the disease. The aim of this study was to determine the drug susceptibility profiles and genetic diversity using the random amplified polymorphic DNA (RAPD) and mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU VNTR) of MTB isolates from sputum samples of pulmonary TB patients residing in the two slums of Jaipur city in Rajasthan, India. Methods: Sputum samples collected from pulmonary TB patients, their contacts and suspects during 2010-2012 were processed for microscopy and mycobacterial culture. Drug susceptibility testing was done by one per cent indirect proportion method on Lowenstein–Jensen medium for first-line anti-TB drugs rifampicin, isoniazid, ethambutol and streptomycin. MTB DNA was extracted by physicochemical method, and DNA fingerprinting was done by RAPD and MIRU VNTR analysis. Results: Among 175 sputum samples collected, 75 were positive (43.8%) for acid-fast bacilli, 83 for MTB culture and four were contaminated. Fifty two isolates (62.7%) were fully sensitive to four drugs, and five (6%) were multidrug resistant (MDR). RAPD analysis of 81 isolates revealed six clusters containing 23 (28.4%) isolates, and 58 (71.6%) were unique. MIRU VNTR analysis clustered 20 (24.7%) isolates, and 61 (75.3%) were unique. Interpretation & conclusions: About 62.7 per cent isolates from the sputum samples from slum areas were sensitive to four drugs; six per cent of isolates were MDR. Poly-resistance other than MDR was high (16%). About one-fourth isolates were clustered by either method. RAPD was rapid, less expensive but had low reproducibility. MIRU VNTR analysis could identify to greater extent the epidemiological link in the population studied. Drug susceptibility testing - mycobacterial interspersed repetitive units-variable number of tandem repeats - Mycobacterium tuberculosis - random amplified polymorphic DNA Medicine R Deepti Dashora verfasserin aut Vipin Kumar verfasserin aut Sumit Goyal verfasserin aut Bhavana Sharma verfasserin aut Madhu Kumar verfasserin aut Kailash Narayan Gupta verfasserin aut Vishnu Dutt Sharma verfasserin aut D S Chauhan verfasserin aut Kiran Katoch verfasserin aut Vishwa Mohan Katoch verfasserin aut In Indian Journal of Medical Research Wolters Kluwer Medknow Publications, 2005 145(2017), 1, Seite 74-83 (DE-627)DOAJ000026956 09715916 nnns volume:145 year:2017 number:1 pages:74-83 https://doi.org/10.4103/ijmr.IJMR_336_14 kostenfrei https://doaj.org/article/a8f99e22da134031b1fa50a51609a241 kostenfrei http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2017;volume=145;issue=1;spage=74;epage=83;aulast=Malhotra kostenfrei https://doaj.org/toc/0971-5916 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 145 2017 1 74-83 |
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Genetic diversity & drug sensitivity profiles of Mycobacterium tuberculosis isolates from two slums of Jaipur city, Rajasthan, India Drug susceptibility testing - mycobacterial interspersed repetitive units-variable number of tandem repeats - Mycobacterium tuberculosis - random amplified polymorphic DNA |
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Genetic diversity & drug sensitivity profiles of Mycobacterium tuberculosis isolates from two slums of Jaipur city, Rajasthan, India |
| abstract |
Background & objectives: Slums are considered as hotspots of tuberculosis (TB). The study of genetic diversity and drug susceptibility profile of Mycobacterium tuberculosis (MTB) will help understand the transmission dynamics and can be used for better prevention and control of the disease. The aim of this study was to determine the drug susceptibility profiles and genetic diversity using the random amplified polymorphic DNA (RAPD) and mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU VNTR) of MTB isolates from sputum samples of pulmonary TB patients residing in the two slums of Jaipur city in Rajasthan, India. Methods: Sputum samples collected from pulmonary TB patients, their contacts and suspects during 2010-2012 were processed for microscopy and mycobacterial culture. Drug susceptibility testing was done by one per cent indirect proportion method on Lowenstein–Jensen medium for first-line anti-TB drugs rifampicin, isoniazid, ethambutol and streptomycin. MTB DNA was extracted by physicochemical method, and DNA fingerprinting was done by RAPD and MIRU VNTR analysis. Results: Among 175 sputum samples collected, 75 were positive (43.8%) for acid-fast bacilli, 83 for MTB culture and four were contaminated. Fifty two isolates (62.7%) were fully sensitive to four drugs, and five (6%) were multidrug resistant (MDR). RAPD analysis of 81 isolates revealed six clusters containing 23 (28.4%) isolates, and 58 (71.6%) were unique. MIRU VNTR analysis clustered 20 (24.7%) isolates, and 61 (75.3%) were unique. Interpretation & conclusions: About 62.7 per cent isolates from the sputum samples from slum areas were sensitive to four drugs; six per cent of isolates were MDR. Poly-resistance other than MDR was high (16%). About one-fourth isolates were clustered by either method. RAPD was rapid, less expensive but had low reproducibility. MIRU VNTR analysis could identify to greater extent the epidemiological link in the population studied. |
| abstractGer |
Background & objectives: Slums are considered as hotspots of tuberculosis (TB). The study of genetic diversity and drug susceptibility profile of Mycobacterium tuberculosis (MTB) will help understand the transmission dynamics and can be used for better prevention and control of the disease. The aim of this study was to determine the drug susceptibility profiles and genetic diversity using the random amplified polymorphic DNA (RAPD) and mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU VNTR) of MTB isolates from sputum samples of pulmonary TB patients residing in the two slums of Jaipur city in Rajasthan, India. Methods: Sputum samples collected from pulmonary TB patients, their contacts and suspects during 2010-2012 were processed for microscopy and mycobacterial culture. Drug susceptibility testing was done by one per cent indirect proportion method on Lowenstein–Jensen medium for first-line anti-TB drugs rifampicin, isoniazid, ethambutol and streptomycin. MTB DNA was extracted by physicochemical method, and DNA fingerprinting was done by RAPD and MIRU VNTR analysis. Results: Among 175 sputum samples collected, 75 were positive (43.8%) for acid-fast bacilli, 83 for MTB culture and four were contaminated. Fifty two isolates (62.7%) were fully sensitive to four drugs, and five (6%) were multidrug resistant (MDR). RAPD analysis of 81 isolates revealed six clusters containing 23 (28.4%) isolates, and 58 (71.6%) were unique. MIRU VNTR analysis clustered 20 (24.7%) isolates, and 61 (75.3%) were unique. Interpretation & conclusions: About 62.7 per cent isolates from the sputum samples from slum areas were sensitive to four drugs; six per cent of isolates were MDR. Poly-resistance other than MDR was high (16%). About one-fourth isolates were clustered by either method. RAPD was rapid, less expensive but had low reproducibility. MIRU VNTR analysis could identify to greater extent the epidemiological link in the population studied. |
| abstract_unstemmed |
Background & objectives: Slums are considered as hotspots of tuberculosis (TB). The study of genetic diversity and drug susceptibility profile of Mycobacterium tuberculosis (MTB) will help understand the transmission dynamics and can be used for better prevention and control of the disease. The aim of this study was to determine the drug susceptibility profiles and genetic diversity using the random amplified polymorphic DNA (RAPD) and mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU VNTR) of MTB isolates from sputum samples of pulmonary TB patients residing in the two slums of Jaipur city in Rajasthan, India. Methods: Sputum samples collected from pulmonary TB patients, their contacts and suspects during 2010-2012 were processed for microscopy and mycobacterial culture. Drug susceptibility testing was done by one per cent indirect proportion method on Lowenstein–Jensen medium for first-line anti-TB drugs rifampicin, isoniazid, ethambutol and streptomycin. MTB DNA was extracted by physicochemical method, and DNA fingerprinting was done by RAPD and MIRU VNTR analysis. Results: Among 175 sputum samples collected, 75 were positive (43.8%) for acid-fast bacilli, 83 for MTB culture and four were contaminated. Fifty two isolates (62.7%) were fully sensitive to four drugs, and five (6%) were multidrug resistant (MDR). RAPD analysis of 81 isolates revealed six clusters containing 23 (28.4%) isolates, and 58 (71.6%) were unique. MIRU VNTR analysis clustered 20 (24.7%) isolates, and 61 (75.3%) were unique. Interpretation & conclusions: About 62.7 per cent isolates from the sputum samples from slum areas were sensitive to four drugs; six per cent of isolates were MDR. Poly-resistance other than MDR was high (16%). About one-fourth isolates were clustered by either method. RAPD was rapid, less expensive but had low reproducibility. MIRU VNTR analysis could identify to greater extent the epidemiological link in the population studied. |
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