Comparison of the Benzanthrone Luminophores: They Are Not Equal for Rapid Examination of <i<Parafasciolopsis fasciolaemorpha</i< (Trematoda: Digenea)

Luminescent derivatives of benzanthrone are becoming more useful based on their light-absorbing and fluorescent-emitting properties. Our previous studies showed that luminescent staining properties of the same benzanthrone dye differ for variable parasite samples. Therefore, two types of benzanthron...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Ilze Rubenina [verfasserIn] Inese Gavarane [verfasserIn] Elena Kirilova [verfasserIn] Ligita Mezaraupe [verfasserIn] Muza Kirjusina [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	trematode moose benzanthrone luminescent dye confocal laser scanning microscopy

Übergeordnetes Werk:	In: Biomolecules - MDPI AG, 2013, 11(2021), 4, p 598
Übergeordnetes Werk:	volume:11 ; year:2021 ; number:4, p 598

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/biom11040598

Katalog-ID:	DOAJ016509978

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520			\|a Luminescent derivatives of benzanthrone are becoming more useful based on their light-absorbing and fluorescent-emitting properties. Our previous studies showed that luminescent staining properties of the same benzanthrone dye differ for variable parasite samples. Therefore, two types of benzanthrone dyes were prepared. One has a strongly basic amidine group and a halogen atom, and the other has an amide moiety and a tertiary amine group. Trematoda <i<Parafasciolopsis fasciolaemorpha</i< is a liver fluke of a moose (<i<Alces alces</i<) and has a significant influence on the health and abundance of the moose population. Staining protocols for parasite <i<P. fasciolaemorpha</i< specific organ or organ systems imaging are mostly time-consuming and labor-intensive. The study aimed to compare the fixation technique and the staining protocol by synthesized benzanthrone luminescent dyes to determine detailed morphology, anatomical arrangement of the organ systems and gross organization of the muscle layers of <i<P. fasciolaemorpha</i< using confocal laser scanning microscopy. Luminophores were tested for samples fixed in different fixatives. Developed dyes and staining protocol resulting in imaging of all parts of trematode without additional sample preparation procedures, which usually are required for parasite examination. Obtained results confirmed that the most qualitative results could be reached using 3-N-(2-piperidinylacetamido)benzanthrone dye which has amide moiety and a tertiary amine group. Based on obtained results, 3-N-(2-piperidinylacetamido)benzanthrone gave more qualitative parasite visualization than 2-bromo-3-N-(N′,N′-dimethylformamidino)benzanthrone.
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Indexfelder

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allfields	10.3390/biom11040598 doi (DE-627)DOAJ016509978 (DE-599)DOAJe095172c2f0f462b871a59030f3071e8 DE-627 ger DE-627 rakwb eng QR1-502 Ilze Rubenina verfasserin aut Comparison of the Benzanthrone Luminophores: They Are Not Equal for Rapid Examination of <i<Parafasciolopsis fasciolaemorpha</i< (Trematoda: Digenea) 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Luminescent derivatives of benzanthrone are becoming more useful based on their light-absorbing and fluorescent-emitting properties. Our previous studies showed that luminescent staining properties of the same benzanthrone dye differ for variable parasite samples. Therefore, two types of benzanthrone dyes were prepared. One has a strongly basic amidine group and a halogen atom, and the other has an amide moiety and a tertiary amine group. Trematoda <i<Parafasciolopsis fasciolaemorpha</i< is a liver fluke of a moose (<i<Alces alces</i<) and has a significant influence on the health and abundance of the moose population. Staining protocols for parasite <i<P. fasciolaemorpha</i< specific organ or organ systems imaging are mostly time-consuming and labor-intensive. The study aimed to compare the fixation technique and the staining protocol by synthesized benzanthrone luminescent dyes to determine detailed morphology, anatomical arrangement of the organ systems and gross organization of the muscle layers of <i<P. fasciolaemorpha</i< using confocal laser scanning microscopy. Luminophores were tested for samples fixed in different fixatives. Developed dyes and staining protocol resulting in imaging of all parts of trematode without additional sample preparation procedures, which usually are required for parasite examination. Obtained results confirmed that the most qualitative results could be reached using 3-N-(2-piperidinylacetamido)benzanthrone dye which has amide moiety and a tertiary amine group. Based on obtained results, 3-N-(2-piperidinylacetamido)benzanthrone gave more qualitative parasite visualization than 2-bromo-3-N-(N′,N′-dimethylformamidino)benzanthrone. <i<Parafasciolopsis fasciolaemorpha</i< trematode moose benzanthrone luminescent dye confocal laser scanning microscopy Microbiology Inese Gavarane verfasserin aut Elena Kirilova verfasserin aut Ligita Mezaraupe verfasserin aut Muza Kirjusina verfasserin aut In Biomolecules MDPI AG, 2013 11(2021), 4, p 598 (DE-627)735688915 (DE-600)2701262-1 2218273X nnns volume:11 year:2021 number:4, p 598 https://doi.org/10.3390/biom11040598 kostenfrei https://doaj.org/article/e095172c2f0f462b871a59030f3071e8 kostenfrei https://www.mdpi.com/2218-273X/11/4/598 kostenfrei https://doaj.org/toc/2218-273X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2021 4, p 598
spelling	10.3390/biom11040598 doi (DE-627)DOAJ016509978 (DE-599)DOAJe095172c2f0f462b871a59030f3071e8 DE-627 ger DE-627 rakwb eng QR1-502 Ilze Rubenina verfasserin aut Comparison of the Benzanthrone Luminophores: They Are Not Equal for Rapid Examination of <i<Parafasciolopsis fasciolaemorpha</i< (Trematoda: Digenea) 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Luminescent derivatives of benzanthrone are becoming more useful based on their light-absorbing and fluorescent-emitting properties. Our previous studies showed that luminescent staining properties of the same benzanthrone dye differ for variable parasite samples. Therefore, two types of benzanthrone dyes were prepared. One has a strongly basic amidine group and a halogen atom, and the other has an amide moiety and a tertiary amine group. Trematoda <i<Parafasciolopsis fasciolaemorpha</i< is a liver fluke of a moose (<i<Alces alces</i<) and has a significant influence on the health and abundance of the moose population. Staining protocols for parasite <i<P. fasciolaemorpha</i< specific organ or organ systems imaging are mostly time-consuming and labor-intensive. The study aimed to compare the fixation technique and the staining protocol by synthesized benzanthrone luminescent dyes to determine detailed morphology, anatomical arrangement of the organ systems and gross organization of the muscle layers of <i<P. fasciolaemorpha</i< using confocal laser scanning microscopy. Luminophores were tested for samples fixed in different fixatives. Developed dyes and staining protocol resulting in imaging of all parts of trematode without additional sample preparation procedures, which usually are required for parasite examination. Obtained results confirmed that the most qualitative results could be reached using 3-N-(2-piperidinylacetamido)benzanthrone dye which has amide moiety and a tertiary amine group. Based on obtained results, 3-N-(2-piperidinylacetamido)benzanthrone gave more qualitative parasite visualization than 2-bromo-3-N-(N′,N′-dimethylformamidino)benzanthrone. <i<Parafasciolopsis fasciolaemorpha</i< trematode moose benzanthrone luminescent dye confocal laser scanning microscopy Microbiology Inese Gavarane verfasserin aut Elena Kirilova verfasserin aut Ligita Mezaraupe verfasserin aut Muza Kirjusina verfasserin aut In Biomolecules MDPI AG, 2013 11(2021), 4, p 598 (DE-627)735688915 (DE-600)2701262-1 2218273X nnns volume:11 year:2021 number:4, p 598 https://doi.org/10.3390/biom11040598 kostenfrei https://doaj.org/article/e095172c2f0f462b871a59030f3071e8 kostenfrei https://www.mdpi.com/2218-273X/11/4/598 kostenfrei https://doaj.org/toc/2218-273X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2021 4, p 598
allfields_unstemmed	10.3390/biom11040598 doi (DE-627)DOAJ016509978 (DE-599)DOAJe095172c2f0f462b871a59030f3071e8 DE-627 ger DE-627 rakwb eng QR1-502 Ilze Rubenina verfasserin aut Comparison of the Benzanthrone Luminophores: They Are Not Equal for Rapid Examination of <i<Parafasciolopsis fasciolaemorpha</i< (Trematoda: Digenea) 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Luminescent derivatives of benzanthrone are becoming more useful based on their light-absorbing and fluorescent-emitting properties. Our previous studies showed that luminescent staining properties of the same benzanthrone dye differ for variable parasite samples. Therefore, two types of benzanthrone dyes were prepared. One has a strongly basic amidine group and a halogen atom, and the other has an amide moiety and a tertiary amine group. Trematoda <i<Parafasciolopsis fasciolaemorpha</i< is a liver fluke of a moose (<i<Alces alces</i<) and has a significant influence on the health and abundance of the moose population. Staining protocols for parasite <i<P. fasciolaemorpha</i< specific organ or organ systems imaging are mostly time-consuming and labor-intensive. The study aimed to compare the fixation technique and the staining protocol by synthesized benzanthrone luminescent dyes to determine detailed morphology, anatomical arrangement of the organ systems and gross organization of the muscle layers of <i<P. fasciolaemorpha</i< using confocal laser scanning microscopy. Luminophores were tested for samples fixed in different fixatives. Developed dyes and staining protocol resulting in imaging of all parts of trematode without additional sample preparation procedures, which usually are required for parasite examination. Obtained results confirmed that the most qualitative results could be reached using 3-N-(2-piperidinylacetamido)benzanthrone dye which has amide moiety and a tertiary amine group. Based on obtained results, 3-N-(2-piperidinylacetamido)benzanthrone gave more qualitative parasite visualization than 2-bromo-3-N-(N′,N′-dimethylformamidino)benzanthrone. <i<Parafasciolopsis fasciolaemorpha</i< trematode moose benzanthrone luminescent dye confocal laser scanning microscopy Microbiology Inese Gavarane verfasserin aut Elena Kirilova verfasserin aut Ligita Mezaraupe verfasserin aut Muza Kirjusina verfasserin aut In Biomolecules MDPI AG, 2013 11(2021), 4, p 598 (DE-627)735688915 (DE-600)2701262-1 2218273X nnns volume:11 year:2021 number:4, p 598 https://doi.org/10.3390/biom11040598 kostenfrei https://doaj.org/article/e095172c2f0f462b871a59030f3071e8 kostenfrei https://www.mdpi.com/2218-273X/11/4/598 kostenfrei https://doaj.org/toc/2218-273X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2021 4, p 598
allfieldsGer	10.3390/biom11040598 doi (DE-627)DOAJ016509978 (DE-599)DOAJe095172c2f0f462b871a59030f3071e8 DE-627 ger DE-627 rakwb eng QR1-502 Ilze Rubenina verfasserin aut Comparison of the Benzanthrone Luminophores: They Are Not Equal for Rapid Examination of <i<Parafasciolopsis fasciolaemorpha</i< (Trematoda: Digenea) 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Luminescent derivatives of benzanthrone are becoming more useful based on their light-absorbing and fluorescent-emitting properties. Our previous studies showed that luminescent staining properties of the same benzanthrone dye differ for variable parasite samples. Therefore, two types of benzanthrone dyes were prepared. One has a strongly basic amidine group and a halogen atom, and the other has an amide moiety and a tertiary amine group. Trematoda <i<Parafasciolopsis fasciolaemorpha</i< is a liver fluke of a moose (<i<Alces alces</i<) and has a significant influence on the health and abundance of the moose population. Staining protocols for parasite <i<P. fasciolaemorpha</i< specific organ or organ systems imaging are mostly time-consuming and labor-intensive. The study aimed to compare the fixation technique and the staining protocol by synthesized benzanthrone luminescent dyes to determine detailed morphology, anatomical arrangement of the organ systems and gross organization of the muscle layers of <i<P. fasciolaemorpha</i< using confocal laser scanning microscopy. Luminophores were tested for samples fixed in different fixatives. Developed dyes and staining protocol resulting in imaging of all parts of trematode without additional sample preparation procedures, which usually are required for parasite examination. Obtained results confirmed that the most qualitative results could be reached using 3-N-(2-piperidinylacetamido)benzanthrone dye which has amide moiety and a tertiary amine group. Based on obtained results, 3-N-(2-piperidinylacetamido)benzanthrone gave more qualitative parasite visualization than 2-bromo-3-N-(N′,N′-dimethylformamidino)benzanthrone. <i<Parafasciolopsis fasciolaemorpha</i< trematode moose benzanthrone luminescent dye confocal laser scanning microscopy Microbiology Inese Gavarane verfasserin aut Elena Kirilova verfasserin aut Ligita Mezaraupe verfasserin aut Muza Kirjusina verfasserin aut In Biomolecules MDPI AG, 2013 11(2021), 4, p 598 (DE-627)735688915 (DE-600)2701262-1 2218273X nnns volume:11 year:2021 number:4, p 598 https://doi.org/10.3390/biom11040598 kostenfrei https://doaj.org/article/e095172c2f0f462b871a59030f3071e8 kostenfrei https://www.mdpi.com/2218-273X/11/4/598 kostenfrei https://doaj.org/toc/2218-273X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2021 4, p 598
allfieldsSound	10.3390/biom11040598 doi (DE-627)DOAJ016509978 (DE-599)DOAJe095172c2f0f462b871a59030f3071e8 DE-627 ger DE-627 rakwb eng QR1-502 Ilze Rubenina verfasserin aut Comparison of the Benzanthrone Luminophores: They Are Not Equal for Rapid Examination of <i<Parafasciolopsis fasciolaemorpha</i< (Trematoda: Digenea) 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Luminescent derivatives of benzanthrone are becoming more useful based on their light-absorbing and fluorescent-emitting properties. Our previous studies showed that luminescent staining properties of the same benzanthrone dye differ for variable parasite samples. Therefore, two types of benzanthrone dyes were prepared. One has a strongly basic amidine group and a halogen atom, and the other has an amide moiety and a tertiary amine group. Trematoda <i<Parafasciolopsis fasciolaemorpha</i< is a liver fluke of a moose (<i<Alces alces</i<) and has a significant influence on the health and abundance of the moose population. Staining protocols for parasite <i<P. fasciolaemorpha</i< specific organ or organ systems imaging are mostly time-consuming and labor-intensive. The study aimed to compare the fixation technique and the staining protocol by synthesized benzanthrone luminescent dyes to determine detailed morphology, anatomical arrangement of the organ systems and gross organization of the muscle layers of <i<P. fasciolaemorpha</i< using confocal laser scanning microscopy. Luminophores were tested for samples fixed in different fixatives. Developed dyes and staining protocol resulting in imaging of all parts of trematode without additional sample preparation procedures, which usually are required for parasite examination. Obtained results confirmed that the most qualitative results could be reached using 3-N-(2-piperidinylacetamido)benzanthrone dye which has amide moiety and a tertiary amine group. Based on obtained results, 3-N-(2-piperidinylacetamido)benzanthrone gave more qualitative parasite visualization than 2-bromo-3-N-(N′,N′-dimethylformamidino)benzanthrone. <i<Parafasciolopsis fasciolaemorpha</i< trematode moose benzanthrone luminescent dye confocal laser scanning microscopy Microbiology Inese Gavarane verfasserin aut Elena Kirilova verfasserin aut Ligita Mezaraupe verfasserin aut Muza Kirjusina verfasserin aut In Biomolecules MDPI AG, 2013 11(2021), 4, p 598 (DE-627)735688915 (DE-600)2701262-1 2218273X nnns volume:11 year:2021 number:4, p 598 https://doi.org/10.3390/biom11040598 kostenfrei https://doaj.org/article/e095172c2f0f462b871a59030f3071e8 kostenfrei https://www.mdpi.com/2218-273X/11/4/598 kostenfrei https://doaj.org/toc/2218-273X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2021 4, p 598
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callnumber-first	Q - Science
author	Ilze Rubenina
spellingShingle	Ilze Rubenina misc QR1-502 misc <i<Parafasciolopsis fasciolaemorpha</i< misc trematode misc moose misc benzanthrone luminescent dye misc confocal laser scanning microscopy misc Microbiology Comparison of the Benzanthrone Luminophores: They Are Not Equal for Rapid Examination of <i<Parafasciolopsis fasciolaemorpha</i< (Trematoda: Digenea)
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topic_title	QR1-502 Comparison of the Benzanthrone Luminophores: They Are Not Equal for Rapid Examination of <i<Parafasciolopsis fasciolaemorpha</i< (Trematoda: Digenea) <i<Parafasciolopsis fasciolaemorpha</i< trematode moose benzanthrone luminescent dye confocal laser scanning microscopy
topic	misc QR1-502 misc <i<Parafasciolopsis fasciolaemorpha</i< misc trematode misc moose misc benzanthrone luminescent dye misc confocal laser scanning microscopy misc Microbiology
topic_unstemmed	misc QR1-502 misc <i<Parafasciolopsis fasciolaemorpha</i< misc trematode misc moose misc benzanthrone luminescent dye misc confocal laser scanning microscopy misc Microbiology
topic_browse	misc QR1-502 misc <i<Parafasciolopsis fasciolaemorpha</i< misc trematode misc moose misc benzanthrone luminescent dye misc confocal laser scanning microscopy misc Microbiology
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title	Comparison of the Benzanthrone Luminophores: They Are Not Equal for Rapid Examination of <i<Parafasciolopsis fasciolaemorpha</i< (Trematoda: Digenea)
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title_full	Comparison of the Benzanthrone Luminophores: They Are Not Equal for Rapid Examination of <i<Parafasciolopsis fasciolaemorpha</i< (Trematoda: Digenea)
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author-letter	Ilze Rubenina
doi_str_mv	10.3390/biom11040598
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title_sort	comparison of the benzanthrone luminophores: they are not equal for rapid examination of <i<parafasciolopsis fasciolaemorpha</i< (trematoda: digenea)
callnumber	QR1-502
title_auth	Comparison of the Benzanthrone Luminophores: They Are Not Equal for Rapid Examination of <i<Parafasciolopsis fasciolaemorpha</i< (Trematoda: Digenea)
abstract	Luminescent derivatives of benzanthrone are becoming more useful based on their light-absorbing and fluorescent-emitting properties. Our previous studies showed that luminescent staining properties of the same benzanthrone dye differ for variable parasite samples. Therefore, two types of benzanthrone dyes were prepared. One has a strongly basic amidine group and a halogen atom, and the other has an amide moiety and a tertiary amine group. Trematoda <i<Parafasciolopsis fasciolaemorpha</i< is a liver fluke of a moose (<i<Alces alces</i<) and has a significant influence on the health and abundance of the moose population. Staining protocols for parasite <i<P. fasciolaemorpha</i< specific organ or organ systems imaging are mostly time-consuming and labor-intensive. The study aimed to compare the fixation technique and the staining protocol by synthesized benzanthrone luminescent dyes to determine detailed morphology, anatomical arrangement of the organ systems and gross organization of the muscle layers of <i<P. fasciolaemorpha</i< using confocal laser scanning microscopy. Luminophores were tested for samples fixed in different fixatives. Developed dyes and staining protocol resulting in imaging of all parts of trematode without additional sample preparation procedures, which usually are required for parasite examination. Obtained results confirmed that the most qualitative results could be reached using 3-N-(2-piperidinylacetamido)benzanthrone dye which has amide moiety and a tertiary amine group. Based on obtained results, 3-N-(2-piperidinylacetamido)benzanthrone gave more qualitative parasite visualization than 2-bromo-3-N-(N′,N′-dimethylformamidino)benzanthrone.
abstractGer	Luminescent derivatives of benzanthrone are becoming more useful based on their light-absorbing and fluorescent-emitting properties. Our previous studies showed that luminescent staining properties of the same benzanthrone dye differ for variable parasite samples. Therefore, two types of benzanthrone dyes were prepared. One has a strongly basic amidine group and a halogen atom, and the other has an amide moiety and a tertiary amine group. Trematoda <i<Parafasciolopsis fasciolaemorpha</i< is a liver fluke of a moose (<i<Alces alces</i<) and has a significant influence on the health and abundance of the moose population. Staining protocols for parasite <i<P. fasciolaemorpha</i< specific organ or organ systems imaging are mostly time-consuming and labor-intensive. The study aimed to compare the fixation technique and the staining protocol by synthesized benzanthrone luminescent dyes to determine detailed morphology, anatomical arrangement of the organ systems and gross organization of the muscle layers of <i<P. fasciolaemorpha</i< using confocal laser scanning microscopy. Luminophores were tested for samples fixed in different fixatives. Developed dyes and staining protocol resulting in imaging of all parts of trematode without additional sample preparation procedures, which usually are required for parasite examination. Obtained results confirmed that the most qualitative results could be reached using 3-N-(2-piperidinylacetamido)benzanthrone dye which has amide moiety and a tertiary amine group. Based on obtained results, 3-N-(2-piperidinylacetamido)benzanthrone gave more qualitative parasite visualization than 2-bromo-3-N-(N′,N′-dimethylformamidino)benzanthrone.
abstract_unstemmed	Luminescent derivatives of benzanthrone are becoming more useful based on their light-absorbing and fluorescent-emitting properties. Our previous studies showed that luminescent staining properties of the same benzanthrone dye differ for variable parasite samples. Therefore, two types of benzanthrone dyes were prepared. One has a strongly basic amidine group and a halogen atom, and the other has an amide moiety and a tertiary amine group. Trematoda <i<Parafasciolopsis fasciolaemorpha</i< is a liver fluke of a moose (<i<Alces alces</i<) and has a significant influence on the health and abundance of the moose population. Staining protocols for parasite <i<P. fasciolaemorpha</i< specific organ or organ systems imaging are mostly time-consuming and labor-intensive. The study aimed to compare the fixation technique and the staining protocol by synthesized benzanthrone luminescent dyes to determine detailed morphology, anatomical arrangement of the organ systems and gross organization of the muscle layers of <i<P. fasciolaemorpha</i< using confocal laser scanning microscopy. Luminophores were tested for samples fixed in different fixatives. Developed dyes and staining protocol resulting in imaging of all parts of trematode without additional sample preparation procedures, which usually are required for parasite examination. Obtained results confirmed that the most qualitative results could be reached using 3-N-(2-piperidinylacetamido)benzanthrone dye which has amide moiety and a tertiary amine group. Based on obtained results, 3-N-(2-piperidinylacetamido)benzanthrone gave more qualitative parasite visualization than 2-bromo-3-N-(N′,N′-dimethylformamidino)benzanthrone.
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title_short	Comparison of the Benzanthrone Luminophores: They Are Not Equal for Rapid Examination of <i<Parafasciolopsis fasciolaemorpha</i< (Trematoda: Digenea)
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Our previous studies showed that luminescent staining properties of the same benzanthrone dye differ for variable parasite samples. Therefore, two types of benzanthrone dyes were prepared. One has a strongly basic amidine group and a halogen atom, and the other has an amide moiety and a tertiary amine group. Trematoda <i<Parafasciolopsis fasciolaemorpha</i< is a liver fluke of a moose (<i<Alces alces</i<) and has a significant influence on the health and abundance of the moose population. Staining protocols for parasite <i<P. fasciolaemorpha</i< specific organ or organ systems imaging are mostly time-consuming and labor-intensive. The study aimed to compare the fixation technique and the staining protocol by synthesized benzanthrone luminescent dyes to determine detailed morphology, anatomical arrangement of the organ systems and gross organization of the muscle layers of <i<P. fasciolaemorpha</i< using confocal laser scanning microscopy. Luminophores were tested for samples fixed in different fixatives. Developed dyes and staining protocol resulting in imaging of all parts of trematode without additional sample preparation procedures, which usually are required for parasite examination. Obtained results confirmed that the most qualitative results could be reached using 3-N-(2-piperidinylacetamido)benzanthrone dye which has amide moiety and a tertiary amine group. 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Comparison of the Benzanthrone Luminophores: They Are Not Equal for Rapid Examination of <i<Parafasciolopsis fasciolaemorpha</i< (Trematoda: Digenea)

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