Fatty acyl availability modulates cardiolipin composition and alters mitochondrial function in HeLa cells

The molecular assembly of cells depends not only on the balance between anabolism and catabolism but to a large degree on the building blocks available in the environment. For cultured mammalian cells, this is largely determined by the composition of the applied growth medium. Here, we study the imp...
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Autor*in:	Gregor Oemer [verfasserIn] Marie-Luise Edenhofer [verfasserIn] Yvonne Wohlfarter [verfasserIn] Katharina Lackner [verfasserIn] Geraldine Leman [verfasserIn] Jakob Koch [verfasserIn] Luiza H.D. Cardoso [verfasserIn] Herbert H. Lindner [verfasserIn] Erich Gnaiger [verfasserIn] Sandrine Dubrac [verfasserIn] Johannes Zschocke [verfasserIn] Markus A. Keller [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	lipids FAs MS cardiolipin mitochondria

Übergeordnetes Werk:	In: Journal of Lipid Research - Elsevier, 2021, 62(2021), Seite 100111-
Übergeordnetes Werk:	volume:62 ; year:2021 ; pages:100111-

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.jlr.2021.100111

Katalog-ID:	DOAJ016512014

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callnumber-first	Q - Science
author	Gregor Oemer
spellingShingle	Gregor Oemer misc QD415-436 misc lipids misc FAs misc MS misc cardiolipin misc mitochondria misc Biochemistry Fatty acyl availability modulates cardiolipin composition and alters mitochondrial function in HeLa cells
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topic_title	QD415-436 Fatty acyl availability modulates cardiolipin composition and alters mitochondrial function in HeLa cells lipids FAs MS cardiolipin mitochondria
topic	misc QD415-436 misc lipids misc FAs misc MS misc cardiolipin misc mitochondria misc Biochemistry
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title	Fatty acyl availability modulates cardiolipin composition and alters mitochondrial function in HeLa cells
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title_full	Fatty acyl availability modulates cardiolipin composition and alters mitochondrial function in HeLa cells
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author_browse	Gregor Oemer Marie-Luise Edenhofer Yvonne Wohlfarter Katharina Lackner Geraldine Leman Jakob Koch Luiza H.D. Cardoso Herbert H. Lindner Erich Gnaiger Sandrine Dubrac Johannes Zschocke Markus A. Keller
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title_sort	fatty acyl availability modulates cardiolipin composition and alters mitochondrial function in hela cells
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title_auth	Fatty acyl availability modulates cardiolipin composition and alters mitochondrial function in HeLa cells
abstract	The molecular assembly of cells depends not only on the balance between anabolism and catabolism but to a large degree on the building blocks available in the environment. For cultured mammalian cells, this is largely determined by the composition of the applied growth medium. Here, we study the impact of lipids in the medium on mitochondrial membrane architecture and function by combining LC-MS/MS lipidomics and functional tests with lipid supplementation experiments in an otherwise serum-free and lipid-free cell culture model. We demonstrate that the composition of mitochondrial cardiolipins strongly depends on the lipid environment in cultured cells and favors the incorporation of essential linoleic acid over other fatty acids. Simultaneously, the mitochondrial respiratory complex I activity was altered, whereas the matrix-localized enzyme citrate synthase was unaffected. This raises the question on a link between membrane composition and respiratory control. In summary, we found a strong dependency of central mitochondrial features on the type of lipids contained in the growth medium. This underlines the importance of considering these factors when using and establishing cell culture models in biomedical research. In summary, we found a strong dependency of central mitochondrial features on the type of lipids contained in the growth medium.
abstractGer	The molecular assembly of cells depends not only on the balance between anabolism and catabolism but to a large degree on the building blocks available in the environment. For cultured mammalian cells, this is largely determined by the composition of the applied growth medium. Here, we study the impact of lipids in the medium on mitochondrial membrane architecture and function by combining LC-MS/MS lipidomics and functional tests with lipid supplementation experiments in an otherwise serum-free and lipid-free cell culture model. We demonstrate that the composition of mitochondrial cardiolipins strongly depends on the lipid environment in cultured cells and favors the incorporation of essential linoleic acid over other fatty acids. Simultaneously, the mitochondrial respiratory complex I activity was altered, whereas the matrix-localized enzyme citrate synthase was unaffected. This raises the question on a link between membrane composition and respiratory control. In summary, we found a strong dependency of central mitochondrial features on the type of lipids contained in the growth medium. This underlines the importance of considering these factors when using and establishing cell culture models in biomedical research. In summary, we found a strong dependency of central mitochondrial features on the type of lipids contained in the growth medium.
abstract_unstemmed	The molecular assembly of cells depends not only on the balance between anabolism and catabolism but to a large degree on the building blocks available in the environment. For cultured mammalian cells, this is largely determined by the composition of the applied growth medium. Here, we study the impact of lipids in the medium on mitochondrial membrane architecture and function by combining LC-MS/MS lipidomics and functional tests with lipid supplementation experiments in an otherwise serum-free and lipid-free cell culture model. We demonstrate that the composition of mitochondrial cardiolipins strongly depends on the lipid environment in cultured cells and favors the incorporation of essential linoleic acid over other fatty acids. Simultaneously, the mitochondrial respiratory complex I activity was altered, whereas the matrix-localized enzyme citrate synthase was unaffected. This raises the question on a link between membrane composition and respiratory control. In summary, we found a strong dependency of central mitochondrial features on the type of lipids contained in the growth medium. This underlines the importance of considering these factors when using and establishing cell culture models in biomedical research. In summary, we found a strong dependency of central mitochondrial features on the type of lipids contained in the growth medium.
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title_short	Fatty acyl availability modulates cardiolipin composition and alters mitochondrial function in HeLa cells
url	https://doi.org/10.1016/j.jlr.2021.100111 https://doaj.org/article/f0d7f8c3e7e84e5b814e2e68fc7f1f75 http://www.sciencedirect.com/science/article/pii/S0022227521000936 https://doaj.org/toc/0022-2275
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up_date	2024-07-03T21:28:22.071Z
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This underlines the importance of considering these factors when using and establishing cell culture models in biomedical research. 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Fatty acyl availability modulates cardiolipin composition and alters mitochondrial function in HeLa cells

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