Recommendations for good practice in MS-based lipidomics

In the last 2 decades, lipidomics has become one of the fastest expanding scientific disciplines in biomedical research. With an increasing number of new research groups to the field, it is even more important to design guidelines for assuring high standards of data quality. The Lipidomics Standards...
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Autor*in:	Harald C. Köfeler [verfasserIn] Robert Ahrends [verfasserIn] Erin S. Baker [verfasserIn] Kim Ekroos [verfasserIn] Xianlin Han [verfasserIn] Nils Hoffmann [verfasserIn] Michal Holčapek [verfasserIn] Markus R. Wenk [verfasserIn] Gerhard Liebisch [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	lipidomics metabolomics MS chromatography ion mobility spectrometry phospholipids

Übergeordnetes Werk:	In: Journal of Lipid Research - Elsevier, 2021, 62(2021), Seite 100138-
Übergeordnetes Werk:	volume:62 ; year:2021 ; pages:100138-

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.jlr.2021.100138

Katalog-ID:	DOAJ016512960

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callnumber-first	Q - Science
author	Harald C. Köfeler
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topic_title	QD415-436 Recommendations for good practice in MS-based lipidomics lipidomics metabolomics MS chromatography ion mobility spectrometry phospholipids
topic	misc QD415-436 misc lipidomics misc metabolomics misc MS misc chromatography misc ion mobility spectrometry misc phospholipids misc Biochemistry
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title	Recommendations for good practice in MS-based lipidomics
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title_full	Recommendations for good practice in MS-based lipidomics
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title_auth	Recommendations for good practice in MS-based lipidomics
abstract	In the last 2 decades, lipidomics has become one of the fastest expanding scientific disciplines in biomedical research. With an increasing number of new research groups to the field, it is even more important to design guidelines for assuring high standards of data quality. The Lipidomics Standards Initiative is a community-based endeavor for the coordination of development of these best practice guidelines in lipidomics and is embedded within the International Lipidomics Society. It is the intention of this review to highlight the most quality-relevant aspects of the lipidomics workflow, including preanalytics, sample preparation, MS, and lipid species identification and quantitation. Furthermore, this review just does not only highlights examples of best practice but also sheds light on strengths, drawbacks, and pitfalls in the lipidomic analysis workflow. While this review is neither designed to be a step-by-step protocol by itself nor dedicated to a specific application of lipidomics, it should nevertheless provide the interested reader with links and original publications to obtain a comprehensive overview concerning the state-of-the-art practices in the field.
abstractGer	In the last 2 decades, lipidomics has become one of the fastest expanding scientific disciplines in biomedical research. With an increasing number of new research groups to the field, it is even more important to design guidelines for assuring high standards of data quality. The Lipidomics Standards Initiative is a community-based endeavor for the coordination of development of these best practice guidelines in lipidomics and is embedded within the International Lipidomics Society. It is the intention of this review to highlight the most quality-relevant aspects of the lipidomics workflow, including preanalytics, sample preparation, MS, and lipid species identification and quantitation. Furthermore, this review just does not only highlights examples of best practice but also sheds light on strengths, drawbacks, and pitfalls in the lipidomic analysis workflow. While this review is neither designed to be a step-by-step protocol by itself nor dedicated to a specific application of lipidomics, it should nevertheless provide the interested reader with links and original publications to obtain a comprehensive overview concerning the state-of-the-art practices in the field.
abstract_unstemmed	In the last 2 decades, lipidomics has become one of the fastest expanding scientific disciplines in biomedical research. With an increasing number of new research groups to the field, it is even more important to design guidelines for assuring high standards of data quality. The Lipidomics Standards Initiative is a community-based endeavor for the coordination of development of these best practice guidelines in lipidomics and is embedded within the International Lipidomics Society. It is the intention of this review to highlight the most quality-relevant aspects of the lipidomics workflow, including preanalytics, sample preparation, MS, and lipid species identification and quantitation. Furthermore, this review just does not only highlights examples of best practice but also sheds light on strengths, drawbacks, and pitfalls in the lipidomic analysis workflow. While this review is neither designed to be a step-by-step protocol by itself nor dedicated to a specific application of lipidomics, it should nevertheless provide the interested reader with links and original publications to obtain a comprehensive overview concerning the state-of-the-art practices in the field.
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title_short	Recommendations for good practice in MS-based lipidomics
url	https://doi.org/10.1016/j.jlr.2021.100138 https://doaj.org/article/92a585f7f3db439c898bcd07c0242453 http://www.sciencedirect.com/science/article/pii/S0022227521001206 https://doaj.org/toc/0022-2275
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