miRNA Expression Is Increased in Serum from Patients with Semantic Variant Primary Progressive Aphasia

Primary progressive aphasia (PPA) damages the parts of the brain that control speech and language. There are three clinical PPA variants: nonfluent/agrammatic (nfvPPA), logopenic (lvPPA) and semantic (svPPA). The pathophysiology underlying PPA variants is not fully understood, including the role of...
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Autor*in:	Maria Serpente [verfasserIn] Laura Ghezzi [verfasserIn] Chiara Fenoglio [verfasserIn] Francesca R. Buccellato [verfasserIn] Giorgio G. Fumagalli [verfasserIn] Emanuela Rotondo [verfasserIn] Marina Arcaro [verfasserIn] Andrea Arighi [verfasserIn] Daniela Galimberti [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	circulating miRNAs semantic variant primary progressive aphasia (svPPA) logopenic variant PPA gene expression

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 23(2022), 15, p 8487
Übergeordnetes Werk:	volume:23 ; year:2022 ; number:15, p 8487

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms23158487

Katalog-ID:	DOAJ016723724

Internformat


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520			\|a Primary progressive aphasia (PPA) damages the parts of the brain that control speech and language. There are three clinical PPA variants: nonfluent/agrammatic (nfvPPA), logopenic (lvPPA) and semantic (svPPA). The pathophysiology underlying PPA variants is not fully understood, including the role of micro (mi)RNAs which were previously shown to play a role in several neurodegenerative diseases. Using a two-step analysis (array and validation through real-time PCR), we investigated the miRNA expression pattern in serum from 54 PPA patients and 18 controls. In the svPPA cohort, we observed a generalized upregulation of miRNAs with miR-106b-5p and miR-133a-3p reaching statistical significance (miR-106b-5p: 2.69 ± 0.89 mean ± SD vs. 1.18 ± 0.28, <i<p</i< < 0.0001; miR-133a-3p: 2.09 ± 0.10 vs. 0.74 ± 0.11 mean ± SD, <i<p</i< = 0.0002). Conversely, in lvPPA, the majority of miRNAs were downregulated. GO enrichment and KEGG pathway analyses revealed that target genes of both miRNAs are involved in pathways potentially relevant for the pathogenesis of neurodegenerative diseases. This is the first study that investigates the expression profile of circulating miRNAs in PPA variant patients. We identified a specific miRNA expression profile in svPPA that could differentiate this pathological condition from other PPA variants. Nevertheless, these preliminary results need to be confirmed in a larger independent cohort.
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allfields	10.3390/ijms23158487 doi (DE-627)DOAJ016723724 (DE-599)DOAJdafe3a8cf3af42ef9e4270529ad35fc1 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Maria Serpente verfasserin aut miRNA Expression Is Increased in Serum from Patients with Semantic Variant Primary Progressive Aphasia 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Primary progressive aphasia (PPA) damages the parts of the brain that control speech and language. There are three clinical PPA variants: nonfluent/agrammatic (nfvPPA), logopenic (lvPPA) and semantic (svPPA). The pathophysiology underlying PPA variants is not fully understood, including the role of micro (mi)RNAs which were previously shown to play a role in several neurodegenerative diseases. Using a two-step analysis (array and validation through real-time PCR), we investigated the miRNA expression pattern in serum from 54 PPA patients and 18 controls. In the svPPA cohort, we observed a generalized upregulation of miRNAs with miR-106b-5p and miR-133a-3p reaching statistical significance (miR-106b-5p: 2.69 ± 0.89 mean ± SD vs. 1.18 ± 0.28, <i<p</i< < 0.0001; miR-133a-3p: 2.09 ± 0.10 vs. 0.74 ± 0.11 mean ± SD, <i<p</i< = 0.0002). Conversely, in lvPPA, the majority of miRNAs were downregulated. GO enrichment and KEGG pathway analyses revealed that target genes of both miRNAs are involved in pathways potentially relevant for the pathogenesis of neurodegenerative diseases. This is the first study that investigates the expression profile of circulating miRNAs in PPA variant patients. We identified a specific miRNA expression profile in svPPA that could differentiate this pathological condition from other PPA variants. Nevertheless, these preliminary results need to be confirmed in a larger independent cohort. circulating miRNAs semantic variant primary progressive aphasia (svPPA) logopenic variant PPA gene expression Biology (General) Chemistry Laura Ghezzi verfasserin aut Chiara Fenoglio verfasserin aut Francesca R. Buccellato verfasserin aut Giorgio G. Fumagalli verfasserin aut Emanuela Rotondo verfasserin aut Marina Arcaro verfasserin aut Andrea Arighi verfasserin aut Daniela Galimberti verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 15, p 8487 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:15, p 8487 https://doi.org/10.3390/ijms23158487 kostenfrei https://doaj.org/article/dafe3a8cf3af42ef9e4270529ad35fc1 kostenfrei https://www.mdpi.com/1422-0067/23/15/8487 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 15, p 8487
spelling	10.3390/ijms23158487 doi (DE-627)DOAJ016723724 (DE-599)DOAJdafe3a8cf3af42ef9e4270529ad35fc1 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Maria Serpente verfasserin aut miRNA Expression Is Increased in Serum from Patients with Semantic Variant Primary Progressive Aphasia 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Primary progressive aphasia (PPA) damages the parts of the brain that control speech and language. There are three clinical PPA variants: nonfluent/agrammatic (nfvPPA), logopenic (lvPPA) and semantic (svPPA). The pathophysiology underlying PPA variants is not fully understood, including the role of micro (mi)RNAs which were previously shown to play a role in several neurodegenerative diseases. Using a two-step analysis (array and validation through real-time PCR), we investigated the miRNA expression pattern in serum from 54 PPA patients and 18 controls. In the svPPA cohort, we observed a generalized upregulation of miRNAs with miR-106b-5p and miR-133a-3p reaching statistical significance (miR-106b-5p: 2.69 ± 0.89 mean ± SD vs. 1.18 ± 0.28, <i<p</i< < 0.0001; miR-133a-3p: 2.09 ± 0.10 vs. 0.74 ± 0.11 mean ± SD, <i<p</i< = 0.0002). Conversely, in lvPPA, the majority of miRNAs were downregulated. GO enrichment and KEGG pathway analyses revealed that target genes of both miRNAs are involved in pathways potentially relevant for the pathogenesis of neurodegenerative diseases. This is the first study that investigates the expression profile of circulating miRNAs in PPA variant patients. We identified a specific miRNA expression profile in svPPA that could differentiate this pathological condition from other PPA variants. Nevertheless, these preliminary results need to be confirmed in a larger independent cohort. circulating miRNAs semantic variant primary progressive aphasia (svPPA) logopenic variant PPA gene expression Biology (General) Chemistry Laura Ghezzi verfasserin aut Chiara Fenoglio verfasserin aut Francesca R. Buccellato verfasserin aut Giorgio G. Fumagalli verfasserin aut Emanuela Rotondo verfasserin aut Marina Arcaro verfasserin aut Andrea Arighi verfasserin aut Daniela Galimberti verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 15, p 8487 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:15, p 8487 https://doi.org/10.3390/ijms23158487 kostenfrei https://doaj.org/article/dafe3a8cf3af42ef9e4270529ad35fc1 kostenfrei https://www.mdpi.com/1422-0067/23/15/8487 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 15, p 8487
allfields_unstemmed	10.3390/ijms23158487 doi (DE-627)DOAJ016723724 (DE-599)DOAJdafe3a8cf3af42ef9e4270529ad35fc1 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Maria Serpente verfasserin aut miRNA Expression Is Increased in Serum from Patients with Semantic Variant Primary Progressive Aphasia 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Primary progressive aphasia (PPA) damages the parts of the brain that control speech and language. There are three clinical PPA variants: nonfluent/agrammatic (nfvPPA), logopenic (lvPPA) and semantic (svPPA). The pathophysiology underlying PPA variants is not fully understood, including the role of micro (mi)RNAs which were previously shown to play a role in several neurodegenerative diseases. Using a two-step analysis (array and validation through real-time PCR), we investigated the miRNA expression pattern in serum from 54 PPA patients and 18 controls. In the svPPA cohort, we observed a generalized upregulation of miRNAs with miR-106b-5p and miR-133a-3p reaching statistical significance (miR-106b-5p: 2.69 ± 0.89 mean ± SD vs. 1.18 ± 0.28, <i<p</i< < 0.0001; miR-133a-3p: 2.09 ± 0.10 vs. 0.74 ± 0.11 mean ± SD, <i<p</i< = 0.0002). Conversely, in lvPPA, the majority of miRNAs were downregulated. GO enrichment and KEGG pathway analyses revealed that target genes of both miRNAs are involved in pathways potentially relevant for the pathogenesis of neurodegenerative diseases. This is the first study that investigates the expression profile of circulating miRNAs in PPA variant patients. We identified a specific miRNA expression profile in svPPA that could differentiate this pathological condition from other PPA variants. Nevertheless, these preliminary results need to be confirmed in a larger independent cohort. circulating miRNAs semantic variant primary progressive aphasia (svPPA) logopenic variant PPA gene expression Biology (General) Chemistry Laura Ghezzi verfasserin aut Chiara Fenoglio verfasserin aut Francesca R. Buccellato verfasserin aut Giorgio G. Fumagalli verfasserin aut Emanuela Rotondo verfasserin aut Marina Arcaro verfasserin aut Andrea Arighi verfasserin aut Daniela Galimberti verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 15, p 8487 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:15, p 8487 https://doi.org/10.3390/ijms23158487 kostenfrei https://doaj.org/article/dafe3a8cf3af42ef9e4270529ad35fc1 kostenfrei https://www.mdpi.com/1422-0067/23/15/8487 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 15, p 8487
allfieldsGer	10.3390/ijms23158487 doi (DE-627)DOAJ016723724 (DE-599)DOAJdafe3a8cf3af42ef9e4270529ad35fc1 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Maria Serpente verfasserin aut miRNA Expression Is Increased in Serum from Patients with Semantic Variant Primary Progressive Aphasia 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Primary progressive aphasia (PPA) damages the parts of the brain that control speech and language. There are three clinical PPA variants: nonfluent/agrammatic (nfvPPA), logopenic (lvPPA) and semantic (svPPA). The pathophysiology underlying PPA variants is not fully understood, including the role of micro (mi)RNAs which were previously shown to play a role in several neurodegenerative diseases. Using a two-step analysis (array and validation through real-time PCR), we investigated the miRNA expression pattern in serum from 54 PPA patients and 18 controls. In the svPPA cohort, we observed a generalized upregulation of miRNAs with miR-106b-5p and miR-133a-3p reaching statistical significance (miR-106b-5p: 2.69 ± 0.89 mean ± SD vs. 1.18 ± 0.28, <i<p</i< < 0.0001; miR-133a-3p: 2.09 ± 0.10 vs. 0.74 ± 0.11 mean ± SD, <i<p</i< = 0.0002). Conversely, in lvPPA, the majority of miRNAs were downregulated. GO enrichment and KEGG pathway analyses revealed that target genes of both miRNAs are involved in pathways potentially relevant for the pathogenesis of neurodegenerative diseases. This is the first study that investigates the expression profile of circulating miRNAs in PPA variant patients. We identified a specific miRNA expression profile in svPPA that could differentiate this pathological condition from other PPA variants. Nevertheless, these preliminary results need to be confirmed in a larger independent cohort. circulating miRNAs semantic variant primary progressive aphasia (svPPA) logopenic variant PPA gene expression Biology (General) Chemistry Laura Ghezzi verfasserin aut Chiara Fenoglio verfasserin aut Francesca R. Buccellato verfasserin aut Giorgio G. Fumagalli verfasserin aut Emanuela Rotondo verfasserin aut Marina Arcaro verfasserin aut Andrea Arighi verfasserin aut Daniela Galimberti verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 15, p 8487 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:15, p 8487 https://doi.org/10.3390/ijms23158487 kostenfrei https://doaj.org/article/dafe3a8cf3af42ef9e4270529ad35fc1 kostenfrei https://www.mdpi.com/1422-0067/23/15/8487 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 15, p 8487
allfieldsSound	10.3390/ijms23158487 doi (DE-627)DOAJ016723724 (DE-599)DOAJdafe3a8cf3af42ef9e4270529ad35fc1 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Maria Serpente verfasserin aut miRNA Expression Is Increased in Serum from Patients with Semantic Variant Primary Progressive Aphasia 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Primary progressive aphasia (PPA) damages the parts of the brain that control speech and language. There are three clinical PPA variants: nonfluent/agrammatic (nfvPPA), logopenic (lvPPA) and semantic (svPPA). The pathophysiology underlying PPA variants is not fully understood, including the role of micro (mi)RNAs which were previously shown to play a role in several neurodegenerative diseases. Using a two-step analysis (array and validation through real-time PCR), we investigated the miRNA expression pattern in serum from 54 PPA patients and 18 controls. In the svPPA cohort, we observed a generalized upregulation of miRNAs with miR-106b-5p and miR-133a-3p reaching statistical significance (miR-106b-5p: 2.69 ± 0.89 mean ± SD vs. 1.18 ± 0.28, <i<p</i< < 0.0001; miR-133a-3p: 2.09 ± 0.10 vs. 0.74 ± 0.11 mean ± SD, <i<p</i< = 0.0002). Conversely, in lvPPA, the majority of miRNAs were downregulated. GO enrichment and KEGG pathway analyses revealed that target genes of both miRNAs are involved in pathways potentially relevant for the pathogenesis of neurodegenerative diseases. This is the first study that investigates the expression profile of circulating miRNAs in PPA variant patients. We identified a specific miRNA expression profile in svPPA that could differentiate this pathological condition from other PPA variants. Nevertheless, these preliminary results need to be confirmed in a larger independent cohort. circulating miRNAs semantic variant primary progressive aphasia (svPPA) logopenic variant PPA gene expression Biology (General) Chemistry Laura Ghezzi verfasserin aut Chiara Fenoglio verfasserin aut Francesca R. Buccellato verfasserin aut Giorgio G. Fumagalli verfasserin aut Emanuela Rotondo verfasserin aut Marina Arcaro verfasserin aut Andrea Arighi verfasserin aut Daniela Galimberti verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 15, p 8487 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:15, p 8487 https://doi.org/10.3390/ijms23158487 kostenfrei https://doaj.org/article/dafe3a8cf3af42ef9e4270529ad35fc1 kostenfrei https://www.mdpi.com/1422-0067/23/15/8487 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 15, p 8487
language	English
source	In International Journal of Molecular Sciences 23(2022), 15, p 8487 volume:23 year:2022 number:15, p 8487
sourceStr	In International Journal of Molecular Sciences 23(2022), 15, p 8487 volume:23 year:2022 number:15, p 8487
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	circulating miRNAs semantic variant primary progressive aphasia (svPPA) logopenic variant PPA gene expression Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Maria Serpente @@aut@@ Laura Ghezzi @@aut@@ Chiara Fenoglio @@aut@@ Francesca R. Buccellato @@aut@@ Giorgio G. Fumagalli @@aut@@ Emanuela Rotondo @@aut@@ Marina Arcaro @@aut@@ Andrea Arighi @@aut@@ Daniela Galimberti @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ016723724
language_de	englisch
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callnumber-first	Q - Science
author	Maria Serpente
spellingShingle	Maria Serpente misc QH301-705.5 misc QD1-999 misc circulating miRNAs misc semantic variant primary progressive aphasia (svPPA) misc logopenic variant PPA misc gene expression misc Biology (General) misc Chemistry miRNA Expression Is Increased in Serum from Patients with Semantic Variant Primary Progressive Aphasia
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topic_title	QH301-705.5 QD1-999 miRNA Expression Is Increased in Serum from Patients with Semantic Variant Primary Progressive Aphasia circulating miRNAs semantic variant primary progressive aphasia (svPPA) logopenic variant PPA gene expression
topic	misc QH301-705.5 misc QD1-999 misc circulating miRNAs misc semantic variant primary progressive aphasia (svPPA) misc logopenic variant PPA misc gene expression misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc circulating miRNAs misc semantic variant primary progressive aphasia (svPPA) misc logopenic variant PPA misc gene expression misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc circulating miRNAs misc semantic variant primary progressive aphasia (svPPA) misc logopenic variant PPA misc gene expression misc Biology (General) misc Chemistry
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title	miRNA Expression Is Increased in Serum from Patients with Semantic Variant Primary Progressive Aphasia
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title_full	miRNA Expression Is Increased in Serum from Patients with Semantic Variant Primary Progressive Aphasia
author_sort	Maria Serpente
journal	International Journal of Molecular Sciences
journalStr	International Journal of Molecular Sciences
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author_browse	Maria Serpente Laura Ghezzi Chiara Fenoglio Francesca R. Buccellato Giorgio G. Fumagalli Emanuela Rotondo Marina Arcaro Andrea Arighi Daniela Galimberti
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author-letter	Maria Serpente
doi_str_mv	10.3390/ijms23158487
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title_sort	mirna expression is increased in serum from patients with semantic variant primary progressive aphasia
callnumber	QH301-705.5
title_auth	miRNA Expression Is Increased in Serum from Patients with Semantic Variant Primary Progressive Aphasia
abstract	Primary progressive aphasia (PPA) damages the parts of the brain that control speech and language. There are three clinical PPA variants: nonfluent/agrammatic (nfvPPA), logopenic (lvPPA) and semantic (svPPA). The pathophysiology underlying PPA variants is not fully understood, including the role of micro (mi)RNAs which were previously shown to play a role in several neurodegenerative diseases. Using a two-step analysis (array and validation through real-time PCR), we investigated the miRNA expression pattern in serum from 54 PPA patients and 18 controls. In the svPPA cohort, we observed a generalized upregulation of miRNAs with miR-106b-5p and miR-133a-3p reaching statistical significance (miR-106b-5p: 2.69 ± 0.89 mean ± SD vs. 1.18 ± 0.28, <i<p</i< < 0.0001; miR-133a-3p: 2.09 ± 0.10 vs. 0.74 ± 0.11 mean ± SD, <i<p</i< = 0.0002). Conversely, in lvPPA, the majority of miRNAs were downregulated. GO enrichment and KEGG pathway analyses revealed that target genes of both miRNAs are involved in pathways potentially relevant for the pathogenesis of neurodegenerative diseases. This is the first study that investigates the expression profile of circulating miRNAs in PPA variant patients. We identified a specific miRNA expression profile in svPPA that could differentiate this pathological condition from other PPA variants. Nevertheless, these preliminary results need to be confirmed in a larger independent cohort.
abstractGer	Primary progressive aphasia (PPA) damages the parts of the brain that control speech and language. There are three clinical PPA variants: nonfluent/agrammatic (nfvPPA), logopenic (lvPPA) and semantic (svPPA). The pathophysiology underlying PPA variants is not fully understood, including the role of micro (mi)RNAs which were previously shown to play a role in several neurodegenerative diseases. Using a two-step analysis (array and validation through real-time PCR), we investigated the miRNA expression pattern in serum from 54 PPA patients and 18 controls. In the svPPA cohort, we observed a generalized upregulation of miRNAs with miR-106b-5p and miR-133a-3p reaching statistical significance (miR-106b-5p: 2.69 ± 0.89 mean ± SD vs. 1.18 ± 0.28, <i<p</i< < 0.0001; miR-133a-3p: 2.09 ± 0.10 vs. 0.74 ± 0.11 mean ± SD, <i<p</i< = 0.0002). Conversely, in lvPPA, the majority of miRNAs were downregulated. GO enrichment and KEGG pathway analyses revealed that target genes of both miRNAs are involved in pathways potentially relevant for the pathogenesis of neurodegenerative diseases. This is the first study that investigates the expression profile of circulating miRNAs in PPA variant patients. We identified a specific miRNA expression profile in svPPA that could differentiate this pathological condition from other PPA variants. Nevertheless, these preliminary results need to be confirmed in a larger independent cohort.
abstract_unstemmed	Primary progressive aphasia (PPA) damages the parts of the brain that control speech and language. There are three clinical PPA variants: nonfluent/agrammatic (nfvPPA), logopenic (lvPPA) and semantic (svPPA). The pathophysiology underlying PPA variants is not fully understood, including the role of micro (mi)RNAs which were previously shown to play a role in several neurodegenerative diseases. Using a two-step analysis (array and validation through real-time PCR), we investigated the miRNA expression pattern in serum from 54 PPA patients and 18 controls. In the svPPA cohort, we observed a generalized upregulation of miRNAs with miR-106b-5p and miR-133a-3p reaching statistical significance (miR-106b-5p: 2.69 ± 0.89 mean ± SD vs. 1.18 ± 0.28, <i<p</i< < 0.0001; miR-133a-3p: 2.09 ± 0.10 vs. 0.74 ± 0.11 mean ± SD, <i<p</i< = 0.0002). Conversely, in lvPPA, the majority of miRNAs were downregulated. GO enrichment and KEGG pathway analyses revealed that target genes of both miRNAs are involved in pathways potentially relevant for the pathogenesis of neurodegenerative diseases. This is the first study that investigates the expression profile of circulating miRNAs in PPA variant patients. We identified a specific miRNA expression profile in svPPA that could differentiate this pathological condition from other PPA variants. Nevertheless, these preliminary results need to be confirmed in a larger independent cohort.
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container_issue	15, p 8487
title_short	miRNA Expression Is Increased in Serum from Patients with Semantic Variant Primary Progressive Aphasia
url	https://doi.org/10.3390/ijms23158487 https://doaj.org/article/dafe3a8cf3af42ef9e4270529ad35fc1 https://www.mdpi.com/1422-0067/23/15/8487 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
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author2	Laura Ghezzi Chiara Fenoglio Francesca R. Buccellato Giorgio G. Fumagalli Emanuela Rotondo Marina Arcaro Andrea Arighi Daniela Galimberti
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up_date	2024-07-03T22:38:23.830Z
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