Ecto-5′-Nucleotidase (CD73) Regulates the Survival of CD8+ T Cells

Ecto-5′-nucleotidase (CD73) is an enzyme present on the surface of tumor cells whose primary described function is the production of extracellular adenosine. Due to the immunosuppressive properties of adenosine, CD73 is being investigated as a target for new antitumor therapies. We and others have d...
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Autor*in:	Mariana V. Rosemblatt [verfasserIn] Brian Parra-Tello [verfasserIn] Pedro Briceño [verfasserIn] Elizabeth Rivas-Yáñez [verfasserIn] Suat Tucer [verfasserIn] Juan Saavedra-Almarza [verfasserIn] Pilar Hörmann [verfasserIn] Brandon A. Martínez [verfasserIn] Álvaro Lladser [verfasserIn] Mario Rosemblatt [verfasserIn] Caglar Cekic [verfasserIn] María Rosa Bono [verfasserIn] Daniela Sauma [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	CD73/NT5E homeostatic CD8+ T cell antigenic activation CD127 (IL7 receptor) CD25

Übergeordnetes Werk:	In: Frontiers in Cell and Developmental Biology - Frontiers Media S.A., 2014, 9(2021)
Übergeordnetes Werk:	volume:9 ; year:2021

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fcell.2021.647058

Katalog-ID:	DOAJ016775236

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Indexfelder

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allfields	10.3389/fcell.2021.647058 doi (DE-627)DOAJ016775236 (DE-599)DOAJefa534712c724d48a1817811922bf25d DE-627 ger DE-627 rakwb eng QH301-705.5 Mariana V. Rosemblatt verfasserin aut Ecto-5′-Nucleotidase (CD73) Regulates the Survival of CD8+ T Cells 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Ecto-5′-nucleotidase (CD73) is an enzyme present on the surface of tumor cells whose primary described function is the production of extracellular adenosine. Due to the immunosuppressive properties of adenosine, CD73 is being investigated as a target for new antitumor therapies. We and others have described that CD73 is present at the surface of different CD8+ T cell subsets. Nonetheless, there is limited information as to whether CD73 affects CD8+ T cell proliferation and survival. In this study, we assessed the impact of CD73 deficiency on CD8+ T cells by analyzing their proliferation and survival in antigenic and homeostatic conditions. Results obtained from adoptive transfer experiments demonstrate a paradoxical role of CD73. On one side, it favors the expression of interleukin-7 receptor α chain on CD8+ T cells and their homeostatic survival; on the other side, it reduces the survival of activated CD8+ T cells under antigenic stimulation. Also, upon in vitro antigenic stimulation, CD73 decreases the expression of interleukin-2 receptor α chain and the anti-apoptotic molecule Bcl-2, findings that may explain the reduced CD8+ T cell survival observed in this condition. These results indicate that CD73 has a dual effect on CD8+ T cells depending on whether they are subject to an antigenic or homeostatic stimulus, and thus, special attention should be given to these aspects when considering CD73 blockade in the design of novel antitumor therapies. CD73/NT5E homeostatic CD8+ T cell antigenic activation CD127 (IL7 receptor) CD25 Biology (General) Mariana V. Rosemblatt verfasserin aut Brian Parra-Tello verfasserin aut Pedro Briceño verfasserin aut Elizabeth Rivas-Yáñez verfasserin aut Suat Tucer verfasserin aut Juan Saavedra-Almarza verfasserin aut Pilar Hörmann verfasserin aut Brandon A. Martínez verfasserin aut Álvaro Lladser verfasserin aut Álvaro Lladser verfasserin aut Mario Rosemblatt verfasserin aut Mario Rosemblatt verfasserin aut Mario Rosemblatt verfasserin aut Caglar Cekic verfasserin aut María Rosa Bono verfasserin aut Daniela Sauma verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 9(2021) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:9 year:2021 https://doi.org/10.3389/fcell.2021.647058 kostenfrei https://doaj.org/article/efa534712c724d48a1817811922bf25d kostenfrei https://www.frontiersin.org/articles/10.3389/fcell.2021.647058/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021
spelling	10.3389/fcell.2021.647058 doi (DE-627)DOAJ016775236 (DE-599)DOAJefa534712c724d48a1817811922bf25d DE-627 ger DE-627 rakwb eng QH301-705.5 Mariana V. Rosemblatt verfasserin aut Ecto-5′-Nucleotidase (CD73) Regulates the Survival of CD8+ T Cells 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Ecto-5′-nucleotidase (CD73) is an enzyme present on the surface of tumor cells whose primary described function is the production of extracellular adenosine. Due to the immunosuppressive properties of adenosine, CD73 is being investigated as a target for new antitumor therapies. We and others have described that CD73 is present at the surface of different CD8+ T cell subsets. Nonetheless, there is limited information as to whether CD73 affects CD8+ T cell proliferation and survival. In this study, we assessed the impact of CD73 deficiency on CD8+ T cells by analyzing their proliferation and survival in antigenic and homeostatic conditions. Results obtained from adoptive transfer experiments demonstrate a paradoxical role of CD73. On one side, it favors the expression of interleukin-7 receptor α chain on CD8+ T cells and their homeostatic survival; on the other side, it reduces the survival of activated CD8+ T cells under antigenic stimulation. Also, upon in vitro antigenic stimulation, CD73 decreases the expression of interleukin-2 receptor α chain and the anti-apoptotic molecule Bcl-2, findings that may explain the reduced CD8+ T cell survival observed in this condition. These results indicate that CD73 has a dual effect on CD8+ T cells depending on whether they are subject to an antigenic or homeostatic stimulus, and thus, special attention should be given to these aspects when considering CD73 blockade in the design of novel antitumor therapies. CD73/NT5E homeostatic CD8+ T cell antigenic activation CD127 (IL7 receptor) CD25 Biology (General) Mariana V. Rosemblatt verfasserin aut Brian Parra-Tello verfasserin aut Pedro Briceño verfasserin aut Elizabeth Rivas-Yáñez verfasserin aut Suat Tucer verfasserin aut Juan Saavedra-Almarza verfasserin aut Pilar Hörmann verfasserin aut Brandon A. Martínez verfasserin aut Álvaro Lladser verfasserin aut Álvaro Lladser verfasserin aut Mario Rosemblatt verfasserin aut Mario Rosemblatt verfasserin aut Mario Rosemblatt verfasserin aut Caglar Cekic verfasserin aut María Rosa Bono verfasserin aut Daniela Sauma verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 9(2021) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:9 year:2021 https://doi.org/10.3389/fcell.2021.647058 kostenfrei https://doaj.org/article/efa534712c724d48a1817811922bf25d kostenfrei https://www.frontiersin.org/articles/10.3389/fcell.2021.647058/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021
allfields_unstemmed	10.3389/fcell.2021.647058 doi (DE-627)DOAJ016775236 (DE-599)DOAJefa534712c724d48a1817811922bf25d DE-627 ger DE-627 rakwb eng QH301-705.5 Mariana V. Rosemblatt verfasserin aut Ecto-5′-Nucleotidase (CD73) Regulates the Survival of CD8+ T Cells 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Ecto-5′-nucleotidase (CD73) is an enzyme present on the surface of tumor cells whose primary described function is the production of extracellular adenosine. Due to the immunosuppressive properties of adenosine, CD73 is being investigated as a target for new antitumor therapies. We and others have described that CD73 is present at the surface of different CD8+ T cell subsets. Nonetheless, there is limited information as to whether CD73 affects CD8+ T cell proliferation and survival. In this study, we assessed the impact of CD73 deficiency on CD8+ T cells by analyzing their proliferation and survival in antigenic and homeostatic conditions. Results obtained from adoptive transfer experiments demonstrate a paradoxical role of CD73. On one side, it favors the expression of interleukin-7 receptor α chain on CD8+ T cells and their homeostatic survival; on the other side, it reduces the survival of activated CD8+ T cells under antigenic stimulation. Also, upon in vitro antigenic stimulation, CD73 decreases the expression of interleukin-2 receptor α chain and the anti-apoptotic molecule Bcl-2, findings that may explain the reduced CD8+ T cell survival observed in this condition. These results indicate that CD73 has a dual effect on CD8+ T cells depending on whether they are subject to an antigenic or homeostatic stimulus, and thus, special attention should be given to these aspects when considering CD73 blockade in the design of novel antitumor therapies. CD73/NT5E homeostatic CD8+ T cell antigenic activation CD127 (IL7 receptor) CD25 Biology (General) Mariana V. Rosemblatt verfasserin aut Brian Parra-Tello verfasserin aut Pedro Briceño verfasserin aut Elizabeth Rivas-Yáñez verfasserin aut Suat Tucer verfasserin aut Juan Saavedra-Almarza verfasserin aut Pilar Hörmann verfasserin aut Brandon A. Martínez verfasserin aut Álvaro Lladser verfasserin aut Álvaro Lladser verfasserin aut Mario Rosemblatt verfasserin aut Mario Rosemblatt verfasserin aut Mario Rosemblatt verfasserin aut Caglar Cekic verfasserin aut María Rosa Bono verfasserin aut Daniela Sauma verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 9(2021) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:9 year:2021 https://doi.org/10.3389/fcell.2021.647058 kostenfrei https://doaj.org/article/efa534712c724d48a1817811922bf25d kostenfrei https://www.frontiersin.org/articles/10.3389/fcell.2021.647058/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021
allfieldsGer	10.3389/fcell.2021.647058 doi (DE-627)DOAJ016775236 (DE-599)DOAJefa534712c724d48a1817811922bf25d DE-627 ger DE-627 rakwb eng QH301-705.5 Mariana V. Rosemblatt verfasserin aut Ecto-5′-Nucleotidase (CD73) Regulates the Survival of CD8+ T Cells 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Ecto-5′-nucleotidase (CD73) is an enzyme present on the surface of tumor cells whose primary described function is the production of extracellular adenosine. Due to the immunosuppressive properties of adenosine, CD73 is being investigated as a target for new antitumor therapies. We and others have described that CD73 is present at the surface of different CD8+ T cell subsets. Nonetheless, there is limited information as to whether CD73 affects CD8+ T cell proliferation and survival. In this study, we assessed the impact of CD73 deficiency on CD8+ T cells by analyzing their proliferation and survival in antigenic and homeostatic conditions. Results obtained from adoptive transfer experiments demonstrate a paradoxical role of CD73. On one side, it favors the expression of interleukin-7 receptor α chain on CD8+ T cells and their homeostatic survival; on the other side, it reduces the survival of activated CD8+ T cells under antigenic stimulation. Also, upon in vitro antigenic stimulation, CD73 decreases the expression of interleukin-2 receptor α chain and the anti-apoptotic molecule Bcl-2, findings that may explain the reduced CD8+ T cell survival observed in this condition. These results indicate that CD73 has a dual effect on CD8+ T cells depending on whether they are subject to an antigenic or homeostatic stimulus, and thus, special attention should be given to these aspects when considering CD73 blockade in the design of novel antitumor therapies. CD73/NT5E homeostatic CD8+ T cell antigenic activation CD127 (IL7 receptor) CD25 Biology (General) Mariana V. Rosemblatt verfasserin aut Brian Parra-Tello verfasserin aut Pedro Briceño verfasserin aut Elizabeth Rivas-Yáñez verfasserin aut Suat Tucer verfasserin aut Juan Saavedra-Almarza verfasserin aut Pilar Hörmann verfasserin aut Brandon A. Martínez verfasserin aut Álvaro Lladser verfasserin aut Álvaro Lladser verfasserin aut Mario Rosemblatt verfasserin aut Mario Rosemblatt verfasserin aut Mario Rosemblatt verfasserin aut Caglar Cekic verfasserin aut María Rosa Bono verfasserin aut Daniela Sauma verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 9(2021) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:9 year:2021 https://doi.org/10.3389/fcell.2021.647058 kostenfrei https://doaj.org/article/efa534712c724d48a1817811922bf25d kostenfrei https://www.frontiersin.org/articles/10.3389/fcell.2021.647058/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021
allfieldsSound	10.3389/fcell.2021.647058 doi (DE-627)DOAJ016775236 (DE-599)DOAJefa534712c724d48a1817811922bf25d DE-627 ger DE-627 rakwb eng QH301-705.5 Mariana V. Rosemblatt verfasserin aut Ecto-5′-Nucleotidase (CD73) Regulates the Survival of CD8+ T Cells 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Ecto-5′-nucleotidase (CD73) is an enzyme present on the surface of tumor cells whose primary described function is the production of extracellular adenosine. Due to the immunosuppressive properties of adenosine, CD73 is being investigated as a target for new antitumor therapies. We and others have described that CD73 is present at the surface of different CD8+ T cell subsets. Nonetheless, there is limited information as to whether CD73 affects CD8+ T cell proliferation and survival. In this study, we assessed the impact of CD73 deficiency on CD8+ T cells by analyzing their proliferation and survival in antigenic and homeostatic conditions. Results obtained from adoptive transfer experiments demonstrate a paradoxical role of CD73. On one side, it favors the expression of interleukin-7 receptor α chain on CD8+ T cells and their homeostatic survival; on the other side, it reduces the survival of activated CD8+ T cells under antigenic stimulation. Also, upon in vitro antigenic stimulation, CD73 decreases the expression of interleukin-2 receptor α chain and the anti-apoptotic molecule Bcl-2, findings that may explain the reduced CD8+ T cell survival observed in this condition. These results indicate that CD73 has a dual effect on CD8+ T cells depending on whether they are subject to an antigenic or homeostatic stimulus, and thus, special attention should be given to these aspects when considering CD73 blockade in the design of novel antitumor therapies. CD73/NT5E homeostatic CD8+ T cell antigenic activation CD127 (IL7 receptor) CD25 Biology (General) Mariana V. Rosemblatt verfasserin aut Brian Parra-Tello verfasserin aut Pedro Briceño verfasserin aut Elizabeth Rivas-Yáñez verfasserin aut Suat Tucer verfasserin aut Juan Saavedra-Almarza verfasserin aut Pilar Hörmann verfasserin aut Brandon A. Martínez verfasserin aut Álvaro Lladser verfasserin aut Álvaro Lladser verfasserin aut Mario Rosemblatt verfasserin aut Mario Rosemblatt verfasserin aut Mario Rosemblatt verfasserin aut Caglar Cekic verfasserin aut María Rosa Bono verfasserin aut Daniela Sauma verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 9(2021) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:9 year:2021 https://doi.org/10.3389/fcell.2021.647058 kostenfrei https://doaj.org/article/efa534712c724d48a1817811922bf25d kostenfrei https://www.frontiersin.org/articles/10.3389/fcell.2021.647058/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021
language	English
source	In Frontiers in Cell and Developmental Biology 9(2021) volume:9 year:2021
sourceStr	In Frontiers in Cell and Developmental Biology 9(2021) volume:9 year:2021
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	CD73/NT5E homeostatic CD8+ T cell antigenic activation CD127 (IL7 receptor) CD25 Biology (General)
isfreeaccess_bool	true
container_title	Frontiers in Cell and Developmental Biology
authorswithroles_txt_mv	Mariana V. Rosemblatt @@aut@@ Brian Parra-Tello @@aut@@ Pedro Briceño @@aut@@ Elizabeth Rivas-Yáñez @@aut@@ Suat Tucer @@aut@@ Juan Saavedra-Almarza @@aut@@ Pilar Hörmann @@aut@@ Brandon A. Martínez @@aut@@ Álvaro Lladser @@aut@@ Mario Rosemblatt @@aut@@ Caglar Cekic @@aut@@ María Rosa Bono @@aut@@ Daniela Sauma @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	770398138
id	DOAJ016775236
language_de	englisch
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callnumber-first	Q - Science
author	Mariana V. Rosemblatt
spellingShingle	Mariana V. Rosemblatt misc QH301-705.5 misc CD73/NT5E misc homeostatic misc CD8+ T cell misc antigenic activation misc CD127 (IL7 receptor) misc CD25 misc Biology (General) Ecto-5′-Nucleotidase (CD73) Regulates the Survival of CD8+ T Cells
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topic_title	QH301-705.5 Ecto-5′-Nucleotidase (CD73) Regulates the Survival of CD8+ T Cells CD73/NT5E homeostatic CD8+ T cell antigenic activation CD127 (IL7 receptor) CD25
topic	misc QH301-705.5 misc CD73/NT5E misc homeostatic misc CD8+ T cell misc antigenic activation misc CD127 (IL7 receptor) misc CD25 misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc CD73/NT5E misc homeostatic misc CD8+ T cell misc antigenic activation misc CD127 (IL7 receptor) misc CD25 misc Biology (General)
topic_browse	misc QH301-705.5 misc CD73/NT5E misc homeostatic misc CD8+ T cell misc antigenic activation misc CD127 (IL7 receptor) misc CD25 misc Biology (General)
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title	Ecto-5′-Nucleotidase (CD73) Regulates the Survival of CD8+ T Cells
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title_full	Ecto-5′-Nucleotidase (CD73) Regulates the Survival of CD8+ T Cells
author_sort	Mariana V. Rosemblatt
journal	Frontiers in Cell and Developmental Biology
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author_browse	Mariana V. Rosemblatt Brian Parra-Tello Pedro Briceño Elizabeth Rivas-Yáñez Suat Tucer Juan Saavedra-Almarza Pilar Hörmann Brandon A. Martínez Álvaro Lladser Mario Rosemblatt Caglar Cekic María Rosa Bono Daniela Sauma
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doi_str_mv	10.3389/fcell.2021.647058
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title_sort	ecto-5′-nucleotidase (cd73) regulates the survival of cd8+ t cells
callnumber	QH301-705.5
title_auth	Ecto-5′-Nucleotidase (CD73) Regulates the Survival of CD8+ T Cells
abstract	Ecto-5′-nucleotidase (CD73) is an enzyme present on the surface of tumor cells whose primary described function is the production of extracellular adenosine. Due to the immunosuppressive properties of adenosine, CD73 is being investigated as a target for new antitumor therapies. We and others have described that CD73 is present at the surface of different CD8+ T cell subsets. Nonetheless, there is limited information as to whether CD73 affects CD8+ T cell proliferation and survival. In this study, we assessed the impact of CD73 deficiency on CD8+ T cells by analyzing their proliferation and survival in antigenic and homeostatic conditions. Results obtained from adoptive transfer experiments demonstrate a paradoxical role of CD73. On one side, it favors the expression of interleukin-7 receptor α chain on CD8+ T cells and their homeostatic survival; on the other side, it reduces the survival of activated CD8+ T cells under antigenic stimulation. Also, upon in vitro antigenic stimulation, CD73 decreases the expression of interleukin-2 receptor α chain and the anti-apoptotic molecule Bcl-2, findings that may explain the reduced CD8+ T cell survival observed in this condition. These results indicate that CD73 has a dual effect on CD8+ T cells depending on whether they are subject to an antigenic or homeostatic stimulus, and thus, special attention should be given to these aspects when considering CD73 blockade in the design of novel antitumor therapies.
abstractGer	Ecto-5′-nucleotidase (CD73) is an enzyme present on the surface of tumor cells whose primary described function is the production of extracellular adenosine. Due to the immunosuppressive properties of adenosine, CD73 is being investigated as a target for new antitumor therapies. We and others have described that CD73 is present at the surface of different CD8+ T cell subsets. Nonetheless, there is limited information as to whether CD73 affects CD8+ T cell proliferation and survival. In this study, we assessed the impact of CD73 deficiency on CD8+ T cells by analyzing their proliferation and survival in antigenic and homeostatic conditions. Results obtained from adoptive transfer experiments demonstrate a paradoxical role of CD73. On one side, it favors the expression of interleukin-7 receptor α chain on CD8+ T cells and their homeostatic survival; on the other side, it reduces the survival of activated CD8+ T cells under antigenic stimulation. Also, upon in vitro antigenic stimulation, CD73 decreases the expression of interleukin-2 receptor α chain and the anti-apoptotic molecule Bcl-2, findings that may explain the reduced CD8+ T cell survival observed in this condition. These results indicate that CD73 has a dual effect on CD8+ T cells depending on whether they are subject to an antigenic or homeostatic stimulus, and thus, special attention should be given to these aspects when considering CD73 blockade in the design of novel antitumor therapies.
abstract_unstemmed	Ecto-5′-nucleotidase (CD73) is an enzyme present on the surface of tumor cells whose primary described function is the production of extracellular adenosine. Due to the immunosuppressive properties of adenosine, CD73 is being investigated as a target for new antitumor therapies. We and others have described that CD73 is present at the surface of different CD8+ T cell subsets. Nonetheless, there is limited information as to whether CD73 affects CD8+ T cell proliferation and survival. In this study, we assessed the impact of CD73 deficiency on CD8+ T cells by analyzing their proliferation and survival in antigenic and homeostatic conditions. Results obtained from adoptive transfer experiments demonstrate a paradoxical role of CD73. On one side, it favors the expression of interleukin-7 receptor α chain on CD8+ T cells and their homeostatic survival; on the other side, it reduces the survival of activated CD8+ T cells under antigenic stimulation. Also, upon in vitro antigenic stimulation, CD73 decreases the expression of interleukin-2 receptor α chain and the anti-apoptotic molecule Bcl-2, findings that may explain the reduced CD8+ T cell survival observed in this condition. These results indicate that CD73 has a dual effect on CD8+ T cells depending on whether they are subject to an antigenic or homeostatic stimulus, and thus, special attention should be given to these aspects when considering CD73 blockade in the design of novel antitumor therapies.
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title_short	Ecto-5′-Nucleotidase (CD73) Regulates the Survival of CD8+ T Cells
url	https://doi.org/10.3389/fcell.2021.647058 https://doaj.org/article/efa534712c724d48a1817811922bf25d https://www.frontiersin.org/articles/10.3389/fcell.2021.647058/full https://doaj.org/toc/2296-634X
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author2	Mariana V. Rosemblatt Brian Parra-Tello Pedro Briceño Elizabeth Rivas-Yáñez Suat Tucer Juan Saavedra-Almarza Pilar Hörmann Brandon A. Martínez Álvaro Lladser Mario Rosemblatt Caglar Cekic María Rosa Bono Daniela Sauma
author2Str	Mariana V. Rosemblatt Brian Parra-Tello Pedro Briceño Elizabeth Rivas-Yáñez Suat Tucer Juan Saavedra-Almarza Pilar Hörmann Brandon A. Martínez Álvaro Lladser Mario Rosemblatt Caglar Cekic María Rosa Bono Daniela Sauma
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up_date	2024-07-03T22:54:17.410Z
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