A serine protease from the midgut of the bumblebee, Bombus ignitus (Hymenoptera: Apidae): cDNA cloning, gene structure, expression and enzyme activity

A serine protease gene was cloned from the bumblebee, Bombus ignitus. The B. ignitus serine protease (BiSP) gene spans 1702 bp and consists of four introns and five exons coding for 250 amino acid residues. Southern blot analysis of genomic DNA suggested that BiSP gene is a single copy gene. The cDN...
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Autor*in:	Young Moo CHOO [verfasserIn] Kwang Sik LEE [verfasserIn] Hyung Joo YOON [verfasserIn] Sang Beom LEE [verfasserIn] Ji Hyeon KIM [verfasserIn] Hung Dae SOHN [verfasserIn] Byung Rae JIN [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2007

Schlagwörter:	apidae baculovirus expression vector bombus ignitus bumblebee cdna cloning enzyme expression gene structure serine protease

Übergeordnetes Werk:	In: European Journal of Entomology - Institute of Entomology, Biology Centre, Czech Academy of Science, 2021, 104(2007), 1, Seite 7
Übergeordnetes Werk:	volume:104 ; year:2007 ; number:1 ; pages:7

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.14411/eje.2007.001

Katalog-ID:	DOAJ017103495

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650		4	\|a bumblebee
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650		4	\|a expression
650		4	\|a gene structure
650		4	\|a serine protease
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allfields	10.14411/eje.2007.001 doi (DE-627)DOAJ017103495 (DE-599)DOAJ44fab591c2f544e88a9a4fe5b8af5ae6 DE-627 ger DE-627 rakwb eng QL1-991 Young Moo CHOO verfasserin aut A serine protease from the midgut of the bumblebee, Bombus ignitus (Hymenoptera: Apidae): cDNA cloning, gene structure, expression and enzyme activity 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A serine protease gene was cloned from the bumblebee, Bombus ignitus. The B. ignitus serine protease (BiSP) gene spans 1702 bp and consists of four introns and five exons coding for 250 amino acid residues. Southern blot analysis of genomic DNA suggested that BiSP gene is a single copy gene. The cDNA encoding BiSP was expressed as a 28-kDa polypeptide in baculovirus-infected insect cells and the recombinant BiSP showed activity in a protease enzyme assay. BiSP was specifically expressed in the midgut of B. ignitus queens, males, and workers, suggesting that the BiSP is a gut enzyme involved in the digestion of dietary proteins. apidae baculovirus expression vector bombus ignitus bumblebee cdna cloning enzyme expression gene structure serine protease Zoology Kwang Sik LEE verfasserin aut Hyung Joo YOON verfasserin aut Sang Beom LEE verfasserin aut Ji Hyeon KIM verfasserin aut Hung Dae SOHN verfasserin aut Byung Rae JIN verfasserin aut In European Journal of Entomology Institute of Entomology, Biology Centre, Czech Academy of Science, 2021 104(2007), 1, Seite 7 (DE-627)478976038 (DE-600)2176324-0 18028829 nnns volume:104 year:2007 number:1 pages:7 https://doi.org/10.14411/eje.2007.001 kostenfrei https://doaj.org/article/44fab591c2f544e88a9a4fe5b8af5ae6 kostenfrei https://www.eje.cz/artkey/eje-200701-0001_A_serine_protease_from_the_midgut_of_the_bumblebee_Bombus_ignitus_Hymenoptera_Apidae_cDNA_cloning_gene_st.php kostenfrei https://doaj.org/toc/1210-5759 Journal toc kostenfrei https://doaj.org/toc/1802-8829 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 104 2007 1 7
spelling	10.14411/eje.2007.001 doi (DE-627)DOAJ017103495 (DE-599)DOAJ44fab591c2f544e88a9a4fe5b8af5ae6 DE-627 ger DE-627 rakwb eng QL1-991 Young Moo CHOO verfasserin aut A serine protease from the midgut of the bumblebee, Bombus ignitus (Hymenoptera: Apidae): cDNA cloning, gene structure, expression and enzyme activity 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A serine protease gene was cloned from the bumblebee, Bombus ignitus. The B. ignitus serine protease (BiSP) gene spans 1702 bp and consists of four introns and five exons coding for 250 amino acid residues. Southern blot analysis of genomic DNA suggested that BiSP gene is a single copy gene. The cDNA encoding BiSP was expressed as a 28-kDa polypeptide in baculovirus-infected insect cells and the recombinant BiSP showed activity in a protease enzyme assay. BiSP was specifically expressed in the midgut of B. ignitus queens, males, and workers, suggesting that the BiSP is a gut enzyme involved in the digestion of dietary proteins. apidae baculovirus expression vector bombus ignitus bumblebee cdna cloning enzyme expression gene structure serine protease Zoology Kwang Sik LEE verfasserin aut Hyung Joo YOON verfasserin aut Sang Beom LEE verfasserin aut Ji Hyeon KIM verfasserin aut Hung Dae SOHN verfasserin aut Byung Rae JIN verfasserin aut In European Journal of Entomology Institute of Entomology, Biology Centre, Czech Academy of Science, 2021 104(2007), 1, Seite 7 (DE-627)478976038 (DE-600)2176324-0 18028829 nnns volume:104 year:2007 number:1 pages:7 https://doi.org/10.14411/eje.2007.001 kostenfrei https://doaj.org/article/44fab591c2f544e88a9a4fe5b8af5ae6 kostenfrei https://www.eje.cz/artkey/eje-200701-0001_A_serine_protease_from_the_midgut_of_the_bumblebee_Bombus_ignitus_Hymenoptera_Apidae_cDNA_cloning_gene_st.php kostenfrei https://doaj.org/toc/1210-5759 Journal toc kostenfrei https://doaj.org/toc/1802-8829 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 104 2007 1 7
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allfieldsGer	10.14411/eje.2007.001 doi (DE-627)DOAJ017103495 (DE-599)DOAJ44fab591c2f544e88a9a4fe5b8af5ae6 DE-627 ger DE-627 rakwb eng QL1-991 Young Moo CHOO verfasserin aut A serine protease from the midgut of the bumblebee, Bombus ignitus (Hymenoptera: Apidae): cDNA cloning, gene structure, expression and enzyme activity 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A serine protease gene was cloned from the bumblebee, Bombus ignitus. The B. ignitus serine protease (BiSP) gene spans 1702 bp and consists of four introns and five exons coding for 250 amino acid residues. Southern blot analysis of genomic DNA suggested that BiSP gene is a single copy gene. The cDNA encoding BiSP was expressed as a 28-kDa polypeptide in baculovirus-infected insect cells and the recombinant BiSP showed activity in a protease enzyme assay. BiSP was specifically expressed in the midgut of B. ignitus queens, males, and workers, suggesting that the BiSP is a gut enzyme involved in the digestion of dietary proteins. apidae baculovirus expression vector bombus ignitus bumblebee cdna cloning enzyme expression gene structure serine protease Zoology Kwang Sik LEE verfasserin aut Hyung Joo YOON verfasserin aut Sang Beom LEE verfasserin aut Ji Hyeon KIM verfasserin aut Hung Dae SOHN verfasserin aut Byung Rae JIN verfasserin aut In European Journal of Entomology Institute of Entomology, Biology Centre, Czech Academy of Science, 2021 104(2007), 1, Seite 7 (DE-627)478976038 (DE-600)2176324-0 18028829 nnns volume:104 year:2007 number:1 pages:7 https://doi.org/10.14411/eje.2007.001 kostenfrei https://doaj.org/article/44fab591c2f544e88a9a4fe5b8af5ae6 kostenfrei https://www.eje.cz/artkey/eje-200701-0001_A_serine_protease_from_the_midgut_of_the_bumblebee_Bombus_ignitus_Hymenoptera_Apidae_cDNA_cloning_gene_st.php kostenfrei https://doaj.org/toc/1210-5759 Journal toc kostenfrei https://doaj.org/toc/1802-8829 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 104 2007 1 7
allfieldsSound	10.14411/eje.2007.001 doi (DE-627)DOAJ017103495 (DE-599)DOAJ44fab591c2f544e88a9a4fe5b8af5ae6 DE-627 ger DE-627 rakwb eng QL1-991 Young Moo CHOO verfasserin aut A serine protease from the midgut of the bumblebee, Bombus ignitus (Hymenoptera: Apidae): cDNA cloning, gene structure, expression and enzyme activity 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A serine protease gene was cloned from the bumblebee, Bombus ignitus. The B. ignitus serine protease (BiSP) gene spans 1702 bp and consists of four introns and five exons coding for 250 amino acid residues. Southern blot analysis of genomic DNA suggested that BiSP gene is a single copy gene. The cDNA encoding BiSP was expressed as a 28-kDa polypeptide in baculovirus-infected insect cells and the recombinant BiSP showed activity in a protease enzyme assay. BiSP was specifically expressed in the midgut of B. ignitus queens, males, and workers, suggesting that the BiSP is a gut enzyme involved in the digestion of dietary proteins. apidae baculovirus expression vector bombus ignitus bumblebee cdna cloning enzyme expression gene structure serine protease Zoology Kwang Sik LEE verfasserin aut Hyung Joo YOON verfasserin aut Sang Beom LEE verfasserin aut Ji Hyeon KIM verfasserin aut Hung Dae SOHN verfasserin aut Byung Rae JIN verfasserin aut In European Journal of Entomology Institute of Entomology, Biology Centre, Czech Academy of Science, 2021 104(2007), 1, Seite 7 (DE-627)478976038 (DE-600)2176324-0 18028829 nnns volume:104 year:2007 number:1 pages:7 https://doi.org/10.14411/eje.2007.001 kostenfrei https://doaj.org/article/44fab591c2f544e88a9a4fe5b8af5ae6 kostenfrei https://www.eje.cz/artkey/eje-200701-0001_A_serine_protease_from_the_midgut_of_the_bumblebee_Bombus_ignitus_Hymenoptera_Apidae_cDNA_cloning_gene_st.php kostenfrei https://doaj.org/toc/1210-5759 Journal toc kostenfrei https://doaj.org/toc/1802-8829 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 104 2007 1 7
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source	In European Journal of Entomology 104(2007), 1, Seite 7 volume:104 year:2007 number:1 pages:7
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topic_facet	apidae baculovirus expression vector bombus ignitus bumblebee cdna cloning enzyme expression gene structure serine protease Zoology
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container_title	European Journal of Entomology
authorswithroles_txt_mv	Young Moo CHOO @@aut@@ Kwang Sik LEE @@aut@@ Hyung Joo YOON @@aut@@ Sang Beom LEE @@aut@@ Ji Hyeon KIM @@aut@@ Hung Dae SOHN @@aut@@ Byung Rae JIN @@aut@@
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callnumber-first	Q - Science
author	Young Moo CHOO
spellingShingle	Young Moo CHOO misc QL1-991 misc apidae misc baculovirus expression vector misc bombus ignitus misc bumblebee misc cdna cloning misc enzyme misc expression misc gene structure misc serine protease misc Zoology A serine protease from the midgut of the bumblebee, Bombus ignitus (Hymenoptera: Apidae): cDNA cloning, gene structure, expression and enzyme activity
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topic_title	QL1-991 A serine protease from the midgut of the bumblebee, Bombus ignitus (Hymenoptera: Apidae): cDNA cloning, gene structure, expression and enzyme activity apidae baculovirus expression vector bombus ignitus bumblebee cdna cloning enzyme expression gene structure serine protease
topic	misc QL1-991 misc apidae misc baculovirus expression vector misc bombus ignitus misc bumblebee misc cdna cloning misc enzyme misc expression misc gene structure misc serine protease misc Zoology
topic_unstemmed	misc QL1-991 misc apidae misc baculovirus expression vector misc bombus ignitus misc bumblebee misc cdna cloning misc enzyme misc expression misc gene structure misc serine protease misc Zoology
topic_browse	misc QL1-991 misc apidae misc baculovirus expression vector misc bombus ignitus misc bumblebee misc cdna cloning misc enzyme misc expression misc gene structure misc serine protease misc Zoology
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title	A serine protease from the midgut of the bumblebee, Bombus ignitus (Hymenoptera: Apidae): cDNA cloning, gene structure, expression and enzyme activity
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title_full	A serine protease from the midgut of the bumblebee, Bombus ignitus (Hymenoptera: Apidae): cDNA cloning, gene structure, expression and enzyme activity
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author_browse	Young Moo CHOO Kwang Sik LEE Hyung Joo YOON Sang Beom LEE Ji Hyeon KIM Hung Dae SOHN Byung Rae JIN
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title_sort	serine protease from the midgut of the bumblebee, bombus ignitus (hymenoptera: apidae): cdna cloning, gene structure, expression and enzyme activity
callnumber	QL1-991
title_auth	A serine protease from the midgut of the bumblebee, Bombus ignitus (Hymenoptera: Apidae): cDNA cloning, gene structure, expression and enzyme activity
abstract	A serine protease gene was cloned from the bumblebee, Bombus ignitus. The B. ignitus serine protease (BiSP) gene spans 1702 bp and consists of four introns and five exons coding for 250 amino acid residues. Southern blot analysis of genomic DNA suggested that BiSP gene is a single copy gene. The cDNA encoding BiSP was expressed as a 28-kDa polypeptide in baculovirus-infected insect cells and the recombinant BiSP showed activity in a protease enzyme assay. BiSP was specifically expressed in the midgut of B. ignitus queens, males, and workers, suggesting that the BiSP is a gut enzyme involved in the digestion of dietary proteins.
abstractGer	A serine protease gene was cloned from the bumblebee, Bombus ignitus. The B. ignitus serine protease (BiSP) gene spans 1702 bp and consists of four introns and five exons coding for 250 amino acid residues. Southern blot analysis of genomic DNA suggested that BiSP gene is a single copy gene. The cDNA encoding BiSP was expressed as a 28-kDa polypeptide in baculovirus-infected insect cells and the recombinant BiSP showed activity in a protease enzyme assay. BiSP was specifically expressed in the midgut of B. ignitus queens, males, and workers, suggesting that the BiSP is a gut enzyme involved in the digestion of dietary proteins.
abstract_unstemmed	A serine protease gene was cloned from the bumblebee, Bombus ignitus. The B. ignitus serine protease (BiSP) gene spans 1702 bp and consists of four introns and five exons coding for 250 amino acid residues. Southern blot analysis of genomic DNA suggested that BiSP gene is a single copy gene. The cDNA encoding BiSP was expressed as a 28-kDa polypeptide in baculovirus-infected insect cells and the recombinant BiSP showed activity in a protease enzyme assay. BiSP was specifically expressed in the midgut of B. ignitus queens, males, and workers, suggesting that the BiSP is a gut enzyme involved in the digestion of dietary proteins.
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title_short	A serine protease from the midgut of the bumblebee, Bombus ignitus (Hymenoptera: Apidae): cDNA cloning, gene structure, expression and enzyme activity
url	https://doi.org/10.14411/eje.2007.001 https://doaj.org/article/44fab591c2f544e88a9a4fe5b8af5ae6 https://www.eje.cz/artkey/eje-200701-0001_A_serine_protease_from_the_midgut_of_the_bumblebee_Bombus_ignitus_Hymenoptera_Apidae_cDNA_cloning_gene_st.php https://doaj.org/toc/1210-5759 https://doaj.org/toc/1802-8829
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up_date	2024-07-04T00:19:39.449Z
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A serine protease from the midgut of the bumblebee, Bombus ignitus (Hymenoptera: Apidae): cDNA cloning, gene structure, expression and enzyme activity

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