To Biofilm or Not to Biofilm?
Aim: The goal of this research is to examine the biofilm forming ability of Staphylococcus aureus and Pseudomonas aeruginosa clinical isolates in different in vitro conditions using Meuller-Hinton and Luria-Bertani broths. Material and methods: 30 strains of Pseudomonas aeruginosa and 30 strains of...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Valentina Živković [verfasserIn] Tomislav Kurevija [verfasserIn] Ivana Haršanji Drenjančević [verfasserIn] Maja Bogdan [verfasserIn] Maja Tomić-Paradžik [verfasserIn] Jasminka Talapko [verfasserIn] Domagoj Drenjančević [verfasserIn] |
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| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2018 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Southeastern European Medical Journal - Faculty of Medicine Osijek, 2019, 2(2018), 1, Seite 12-19 |
|---|---|
| Übergeordnetes Werk: |
volume:2 ; year:2018 ; number:1 ; pages:12-19 |
| Links: |
|---|
| DOI / URN: |
10.26332/seemedj.v2i1.69 |
|---|
| Katalog-ID: |
DOAJ017122872 |
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| 520 | |a Aim: The goal of this research is to examine the biofilm forming ability of Staphylococcus aureus and Pseudomonas aeruginosa clinical isolates in different in vitro conditions using Meuller-Hinton and Luria-Bertani broths. Material and methods: 30 strains of Pseudomonas aeruginosa and 30 strains of Staphylococcus aureus obtained from clinical specimens were used. After preparing the suspensions of bacteria inoculated on broths, they were set on microtiter plates and the biofilm production was measured using the spectrophotometric reader on 550 nm. Strains were classified into four categories: non producing, weak producers, moderate and strong producers, based on the comparison of the optical density of samples and negative control. Results: Both of the tested strains, successfully formed a biofilm in both broths (p<0.01). P. aeruginosa strains in both in vitro conditions had a higher percentage of strong producers, in comparison with S. aureus strains (3.3% vs 50%). Nevertheless, there is no statistically significant difference in the biofilm formation between the strains, regardless the used broths and there is no statistically significant difference between the biofilm forming ability of both species observed separately regarding in vitro conditions as well. Conclusion: Both species have an ability to produce biofilm, which likely contributes the pathogenicity and virulence of these bacteria and leads to better understanding their in vivo characteristics to cause infections related to biofilm, as well. | ||
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10.26332/seemedj.v2i1.69 doi (DE-627)DOAJ017122872 (DE-599)DOAJdfd780c9624849af856adc2df62b3c6b DE-627 ger DE-627 rakwb eng Valentina Živković verfasserin aut To Biofilm or Not to Biofilm? 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aim: The goal of this research is to examine the biofilm forming ability of Staphylococcus aureus and Pseudomonas aeruginosa clinical isolates in different in vitro conditions using Meuller-Hinton and Luria-Bertani broths. Material and methods: 30 strains of Pseudomonas aeruginosa and 30 strains of Staphylococcus aureus obtained from clinical specimens were used. After preparing the suspensions of bacteria inoculated on broths, they were set on microtiter plates and the biofilm production was measured using the spectrophotometric reader on 550 nm. Strains were classified into four categories: non producing, weak producers, moderate and strong producers, based on the comparison of the optical density of samples and negative control. Results: Both of the tested strains, successfully formed a biofilm in both broths (p<0.01). P. aeruginosa strains in both in vitro conditions had a higher percentage of strong producers, in comparison with S. aureus strains (3.3% vs 50%). Nevertheless, there is no statistically significant difference in the biofilm formation between the strains, regardless the used broths and there is no statistically significant difference between the biofilm forming ability of both species observed separately regarding in vitro conditions as well. Conclusion: Both species have an ability to produce biofilm, which likely contributes the pathogenicity and virulence of these bacteria and leads to better understanding their in vivo characteristics to cause infections related to biofilm, as well. biofilm P. aeruginosa S. aureus Medicine R Tomislav Kurevija verfasserin aut Ivana Haršanji Drenjančević verfasserin aut Maja Bogdan verfasserin aut Maja Tomić-Paradžik verfasserin aut Jasminka Talapko verfasserin aut Domagoj Drenjančević verfasserin aut In Southeastern European Medical Journal Faculty of Medicine Osijek, 2019 2(2018), 1, Seite 12-19 (DE-627)176059847X (DE-600)3068913-2 24599484 nnns volume:2 year:2018 number:1 pages:12-19 https://doi.org/10.26332/seemedj.v2i1.69 kostenfrei https://doaj.org/article/dfd780c9624849af856adc2df62b3c6b kostenfrei http://seemedj.mefos.unios.hr/index.php/seemedj/article/view/69/32 kostenfrei https://doaj.org/toc/2459-9484 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 2 2018 1 12-19 |
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10.26332/seemedj.v2i1.69 doi (DE-627)DOAJ017122872 (DE-599)DOAJdfd780c9624849af856adc2df62b3c6b DE-627 ger DE-627 rakwb eng Valentina Živković verfasserin aut To Biofilm or Not to Biofilm? 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aim: The goal of this research is to examine the biofilm forming ability of Staphylococcus aureus and Pseudomonas aeruginosa clinical isolates in different in vitro conditions using Meuller-Hinton and Luria-Bertani broths. Material and methods: 30 strains of Pseudomonas aeruginosa and 30 strains of Staphylococcus aureus obtained from clinical specimens were used. After preparing the suspensions of bacteria inoculated on broths, they were set on microtiter plates and the biofilm production was measured using the spectrophotometric reader on 550 nm. Strains were classified into four categories: non producing, weak producers, moderate and strong producers, based on the comparison of the optical density of samples and negative control. Results: Both of the tested strains, successfully formed a biofilm in both broths (p<0.01). P. aeruginosa strains in both in vitro conditions had a higher percentage of strong producers, in comparison with S. aureus strains (3.3% vs 50%). Nevertheless, there is no statistically significant difference in the biofilm formation between the strains, regardless the used broths and there is no statistically significant difference between the biofilm forming ability of both species observed separately regarding in vitro conditions as well. Conclusion: Both species have an ability to produce biofilm, which likely contributes the pathogenicity and virulence of these bacteria and leads to better understanding their in vivo characteristics to cause infections related to biofilm, as well. biofilm P. aeruginosa S. aureus Medicine R Tomislav Kurevija verfasserin aut Ivana Haršanji Drenjančević verfasserin aut Maja Bogdan verfasserin aut Maja Tomić-Paradžik verfasserin aut Jasminka Talapko verfasserin aut Domagoj Drenjančević verfasserin aut In Southeastern European Medical Journal Faculty of Medicine Osijek, 2019 2(2018), 1, Seite 12-19 (DE-627)176059847X (DE-600)3068913-2 24599484 nnns volume:2 year:2018 number:1 pages:12-19 https://doi.org/10.26332/seemedj.v2i1.69 kostenfrei https://doaj.org/article/dfd780c9624849af856adc2df62b3c6b kostenfrei http://seemedj.mefos.unios.hr/index.php/seemedj/article/view/69/32 kostenfrei https://doaj.org/toc/2459-9484 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 2 2018 1 12-19 |
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10.26332/seemedj.v2i1.69 doi (DE-627)DOAJ017122872 (DE-599)DOAJdfd780c9624849af856adc2df62b3c6b DE-627 ger DE-627 rakwb eng Valentina Živković verfasserin aut To Biofilm or Not to Biofilm? 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aim: The goal of this research is to examine the biofilm forming ability of Staphylococcus aureus and Pseudomonas aeruginosa clinical isolates in different in vitro conditions using Meuller-Hinton and Luria-Bertani broths. Material and methods: 30 strains of Pseudomonas aeruginosa and 30 strains of Staphylococcus aureus obtained from clinical specimens were used. After preparing the suspensions of bacteria inoculated on broths, they were set on microtiter plates and the biofilm production was measured using the spectrophotometric reader on 550 nm. Strains were classified into four categories: non producing, weak producers, moderate and strong producers, based on the comparison of the optical density of samples and negative control. Results: Both of the tested strains, successfully formed a biofilm in both broths (p<0.01). P. aeruginosa strains in both in vitro conditions had a higher percentage of strong producers, in comparison with S. aureus strains (3.3% vs 50%). Nevertheless, there is no statistically significant difference in the biofilm formation between the strains, regardless the used broths and there is no statistically significant difference between the biofilm forming ability of both species observed separately regarding in vitro conditions as well. Conclusion: Both species have an ability to produce biofilm, which likely contributes the pathogenicity and virulence of these bacteria and leads to better understanding their in vivo characteristics to cause infections related to biofilm, as well. biofilm P. aeruginosa S. aureus Medicine R Tomislav Kurevija verfasserin aut Ivana Haršanji Drenjančević verfasserin aut Maja Bogdan verfasserin aut Maja Tomić-Paradžik verfasserin aut Jasminka Talapko verfasserin aut Domagoj Drenjančević verfasserin aut In Southeastern European Medical Journal Faculty of Medicine Osijek, 2019 2(2018), 1, Seite 12-19 (DE-627)176059847X (DE-600)3068913-2 24599484 nnns volume:2 year:2018 number:1 pages:12-19 https://doi.org/10.26332/seemedj.v2i1.69 kostenfrei https://doaj.org/article/dfd780c9624849af856adc2df62b3c6b kostenfrei http://seemedj.mefos.unios.hr/index.php/seemedj/article/view/69/32 kostenfrei https://doaj.org/toc/2459-9484 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 2 2018 1 12-19 |
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10.26332/seemedj.v2i1.69 doi (DE-627)DOAJ017122872 (DE-599)DOAJdfd780c9624849af856adc2df62b3c6b DE-627 ger DE-627 rakwb eng Valentina Živković verfasserin aut To Biofilm or Not to Biofilm? 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aim: The goal of this research is to examine the biofilm forming ability of Staphylococcus aureus and Pseudomonas aeruginosa clinical isolates in different in vitro conditions using Meuller-Hinton and Luria-Bertani broths. Material and methods: 30 strains of Pseudomonas aeruginosa and 30 strains of Staphylococcus aureus obtained from clinical specimens were used. After preparing the suspensions of bacteria inoculated on broths, they were set on microtiter plates and the biofilm production was measured using the spectrophotometric reader on 550 nm. Strains were classified into four categories: non producing, weak producers, moderate and strong producers, based on the comparison of the optical density of samples and negative control. Results: Both of the tested strains, successfully formed a biofilm in both broths (p<0.01). P. aeruginosa strains in both in vitro conditions had a higher percentage of strong producers, in comparison with S. aureus strains (3.3% vs 50%). Nevertheless, there is no statistically significant difference in the biofilm formation between the strains, regardless the used broths and there is no statistically significant difference between the biofilm forming ability of both species observed separately regarding in vitro conditions as well. Conclusion: Both species have an ability to produce biofilm, which likely contributes the pathogenicity and virulence of these bacteria and leads to better understanding their in vivo characteristics to cause infections related to biofilm, as well. biofilm P. aeruginosa S. aureus Medicine R Tomislav Kurevija verfasserin aut Ivana Haršanji Drenjančević verfasserin aut Maja Bogdan verfasserin aut Maja Tomić-Paradžik verfasserin aut Jasminka Talapko verfasserin aut Domagoj Drenjančević verfasserin aut In Southeastern European Medical Journal Faculty of Medicine Osijek, 2019 2(2018), 1, Seite 12-19 (DE-627)176059847X (DE-600)3068913-2 24599484 nnns volume:2 year:2018 number:1 pages:12-19 https://doi.org/10.26332/seemedj.v2i1.69 kostenfrei https://doaj.org/article/dfd780c9624849af856adc2df62b3c6b kostenfrei http://seemedj.mefos.unios.hr/index.php/seemedj/article/view/69/32 kostenfrei https://doaj.org/toc/2459-9484 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 2 2018 1 12-19 |
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To Biofilm or Not to Biofilm? |
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To Biofilm or Not to Biofilm? |
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Valentina Živković |
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Southeastern European Medical Journal |
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Southeastern European Medical Journal |
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eng |
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Valentina Živković Tomislav Kurevija Ivana Haršanji Drenjančević Maja Bogdan Maja Tomić-Paradžik Jasminka Talapko Domagoj Drenjančević |
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Elektronische Aufsätze |
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Valentina Živković |
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10.26332/seemedj.v2i1.69 |
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verfasserin |
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biofilm or not to biofilm? |
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To Biofilm or Not to Biofilm? |
| abstract |
Aim: The goal of this research is to examine the biofilm forming ability of Staphylococcus aureus and Pseudomonas aeruginosa clinical isolates in different in vitro conditions using Meuller-Hinton and Luria-Bertani broths. Material and methods: 30 strains of Pseudomonas aeruginosa and 30 strains of Staphylococcus aureus obtained from clinical specimens were used. After preparing the suspensions of bacteria inoculated on broths, they were set on microtiter plates and the biofilm production was measured using the spectrophotometric reader on 550 nm. Strains were classified into four categories: non producing, weak producers, moderate and strong producers, based on the comparison of the optical density of samples and negative control. Results: Both of the tested strains, successfully formed a biofilm in both broths (p<0.01). P. aeruginosa strains in both in vitro conditions had a higher percentage of strong producers, in comparison with S. aureus strains (3.3% vs 50%). Nevertheless, there is no statistically significant difference in the biofilm formation between the strains, regardless the used broths and there is no statistically significant difference between the biofilm forming ability of both species observed separately regarding in vitro conditions as well. Conclusion: Both species have an ability to produce biofilm, which likely contributes the pathogenicity and virulence of these bacteria and leads to better understanding their in vivo characteristics to cause infections related to biofilm, as well. |
| abstractGer |
Aim: The goal of this research is to examine the biofilm forming ability of Staphylococcus aureus and Pseudomonas aeruginosa clinical isolates in different in vitro conditions using Meuller-Hinton and Luria-Bertani broths. Material and methods: 30 strains of Pseudomonas aeruginosa and 30 strains of Staphylococcus aureus obtained from clinical specimens were used. After preparing the suspensions of bacteria inoculated on broths, they were set on microtiter plates and the biofilm production was measured using the spectrophotometric reader on 550 nm. Strains were classified into four categories: non producing, weak producers, moderate and strong producers, based on the comparison of the optical density of samples and negative control. Results: Both of the tested strains, successfully formed a biofilm in both broths (p<0.01). P. aeruginosa strains in both in vitro conditions had a higher percentage of strong producers, in comparison with S. aureus strains (3.3% vs 50%). Nevertheless, there is no statistically significant difference in the biofilm formation between the strains, regardless the used broths and there is no statistically significant difference between the biofilm forming ability of both species observed separately regarding in vitro conditions as well. Conclusion: Both species have an ability to produce biofilm, which likely contributes the pathogenicity and virulence of these bacteria and leads to better understanding their in vivo characteristics to cause infections related to biofilm, as well. |
| abstract_unstemmed |
Aim: The goal of this research is to examine the biofilm forming ability of Staphylococcus aureus and Pseudomonas aeruginosa clinical isolates in different in vitro conditions using Meuller-Hinton and Luria-Bertani broths. Material and methods: 30 strains of Pseudomonas aeruginosa and 30 strains of Staphylococcus aureus obtained from clinical specimens were used. After preparing the suspensions of bacteria inoculated on broths, they were set on microtiter plates and the biofilm production was measured using the spectrophotometric reader on 550 nm. Strains were classified into four categories: non producing, weak producers, moderate and strong producers, based on the comparison of the optical density of samples and negative control. Results: Both of the tested strains, successfully formed a biofilm in both broths (p<0.01). P. aeruginosa strains in both in vitro conditions had a higher percentage of strong producers, in comparison with S. aureus strains (3.3% vs 50%). Nevertheless, there is no statistically significant difference in the biofilm formation between the strains, regardless the used broths and there is no statistically significant difference between the biofilm forming ability of both species observed separately regarding in vitro conditions as well. Conclusion: Both species have an ability to produce biofilm, which likely contributes the pathogenicity and virulence of these bacteria and leads to better understanding their in vivo characteristics to cause infections related to biofilm, as well. |
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To Biofilm or Not to Biofilm? |
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https://doi.org/10.26332/seemedj.v2i1.69 https://doaj.org/article/dfd780c9624849af856adc2df62b3c6b http://seemedj.mefos.unios.hr/index.php/seemedj/article/view/69/32 https://doaj.org/toc/2459-9484 |
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Tomislav Kurevija Ivana Haršanji Drenjančević Maja Bogdan Maja Tomić-Paradžik Jasminka Talapko Domagoj Drenjančević |
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Tomislav Kurevija Ivana Haršanji Drenjančević Maja Bogdan Maja Tomić-Paradžik Jasminka Talapko Domagoj Drenjančević |
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2024-07-04T00:24:18.674Z |
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