Association of MTHFR C677T and MTRR A66G Gene Polymorphisms with Iranian Male Infertility and Its Effect on Seminal Folate and Vitamin B12

Background: The relation between key enzymes in regulation of folate metabolism and male infertility is the subjectof numerous studies. We aimed to determine whether 5, 10-methylenetetrahydrofolate reductase (MTHFR) C677Tand methionine synthase reductase (MTRR) A66G genotypes are associated with mal...
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Autor*in:	Mozhgan Raigani [verfasserIn] Niknam Lakpour [verfasserIn] Meysam Soleimani [verfasserIn] Behrooz Johari [verfasserIn] Mohammad Reza Sadeghi [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	folate male infertility mthfr mtrr vitamin b12

Übergeordnetes Werk:	In: International Journal of Fertility and Sterility - Royan Institute (ACECR), Tehran, 2012, 15(2021), 1, Seite 20-25
Übergeordnetes Werk:	volume:15 ; year:2021 ; number:1 ; pages:20-25

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.22074/ijfs.2021.6155

Katalog-ID:	DOAJ017288770

Internformat


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245	1	0	\|a Association of MTHFR C677T and MTRR A66G Gene Polymorphisms with Iranian Male Infertility and Its Effect on Seminal Folate and Vitamin B12
264		1	\|c 2021
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520			\|a Background: The relation between key enzymes in regulation of folate metabolism and male infertility is the subjectof numerous studies. We aimed to determine whether 5, 10-methylenetetrahydrofolate reductase (MTHFR) C677Tand methionine synthase reductase (MTRR) A66G genotypes are associated with male infertility in Iranian men andto evaluate its effect on seminal levels of folate and vitamin B12.Materials and Methods: In this retrospective study, semen and peripheral blood samples were collected from 254men with oligoasthenoteratozoospermia (OAT) and 77 normozoospermic men who attended Avicenna infertility clinic.Single nucleotide polymorphism (SNP) analysis was carried out in genomic DNA by polymerase chain reaction(PCR)-restriction fragment length polymorphism (RFLP) method for MTHFR C677T and MTRR A66G gene polymorphisms.Results: In MTHFR C677T, our founding showed that T carrier was conversely lower in OAT than normozoospermic men(χ2-test=7.245, P=0.02) whereas in MTRR A66G, A and G carrier showed no significant difference between the two groups(χ2-test=1.079, P=0.53). The concentration of seminal folate was not different between normozoospermic (18.83 ± 17.1 ng/ml) and OAT (16.96 ± 14.2 ng/ml) men (P=0.47). The concentration of vitamin B12 was slightly higher in normozospermicmen (522.6 ± 388.1 pg/ml) compared to OAT men (412.9 ± 303.6 pg/ml, P=0.058).Conclusion: The MTHFR C677T and MTRR A66G have no effect on the concentrations of seminal folate and vitaminB12. The present study showed that two SNPs of MTRR A66G and MTHFR C677T cannot be seen as a risk factor formale factor subfertility.
650		4	\|a folate
650		4	\|a male infertility
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700	0		\|a Niknam Lakpour \|e verfasserin \|4 aut
700	0		\|a Meysam Soleimani \|e verfasserin \|4 aut
700	0		\|a Behrooz Johari \|e verfasserin \|4 aut
700	0		\|a Mohammad Reza Sadeghi \|e verfasserin \|4 aut
773	0	8	\|i In \|t International Journal of Fertility and Sterility \|d Royan Institute (ACECR), Tehran, 2012 \|g 15(2021), 1, Seite 20-25 \|w (DE-627)634381083 \|w (DE-600)2570865-X \|x 20080778 \|7 nnns
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Indexfelder

author_variant	m r mr n l nl m s ms b j bj m r s mrs
matchkey_str	article:20080778:2021----::soitoomhr67adtr6geeoyopimwtiainaenetltadtef
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publishDate	2021
allfields	10.22074/ijfs.2021.6155 doi (DE-627)DOAJ017288770 (DE-599)DOAJc75d66948c6d4e8cbdafb711c04d6f4f DE-627 ger DE-627 rakwb eng R5-920 Mozhgan Raigani verfasserin aut Association of MTHFR C677T and MTRR A66G Gene Polymorphisms with Iranian Male Infertility and Its Effect on Seminal Folate and Vitamin B12 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The relation between key enzymes in regulation of folate metabolism and male infertility is the subjectof numerous studies. We aimed to determine whether 5, 10-methylenetetrahydrofolate reductase (MTHFR) C677Tand methionine synthase reductase (MTRR) A66G genotypes are associated with male infertility in Iranian men andto evaluate its effect on seminal levels of folate and vitamin B12.Materials and Methods: In this retrospective study, semen and peripheral blood samples were collected from 254men with oligoasthenoteratozoospermia (OAT) and 77 normozoospermic men who attended Avicenna infertility clinic.Single nucleotide polymorphism (SNP) analysis was carried out in genomic DNA by polymerase chain reaction(PCR)-restriction fragment length polymorphism (RFLP) method for MTHFR C677T and MTRR A66G gene polymorphisms.Results: In MTHFR C677T, our founding showed that T carrier was conversely lower in OAT than normozoospermic men(χ2-test=7.245, P=0.02) whereas in MTRR A66G, A and G carrier showed no significant difference between the two groups(χ2-test=1.079, P=0.53). The concentration of seminal folate was not different between normozoospermic (18.83 ± 17.1 ng/ml) and OAT (16.96 ± 14.2 ng/ml) men (P=0.47). The concentration of vitamin B12 was slightly higher in normozospermicmen (522.6 ± 388.1 pg/ml) compared to OAT men (412.9 ± 303.6 pg/ml, P=0.058).Conclusion: The MTHFR C677T and MTRR A66G have no effect on the concentrations of seminal folate and vitaminB12. The present study showed that two SNPs of MTRR A66G and MTHFR C677T cannot be seen as a risk factor formale factor subfertility. folate male infertility mthfr mtrr vitamin b12 Medicine (General) Niknam Lakpour verfasserin aut Meysam Soleimani verfasserin aut Behrooz Johari verfasserin aut Mohammad Reza Sadeghi verfasserin aut In International Journal of Fertility and Sterility Royan Institute (ACECR), Tehran, 2012 15(2021), 1, Seite 20-25 (DE-627)634381083 (DE-600)2570865-X 20080778 nnns volume:15 year:2021 number:1 pages:20-25 https://doi.org/10.22074/ijfs.2021.6155 kostenfrei https://doaj.org/article/c75d66948c6d4e8cbdafb711c04d6f4f kostenfrei https://www.ijfs.ir/article_46236_dfc22628b49c661aa79230eece8bbf0b.pdf kostenfrei https://doaj.org/toc/2008-076X Journal toc kostenfrei https://doaj.org/toc/2008-0778 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2021 1 20-25
spelling	10.22074/ijfs.2021.6155 doi (DE-627)DOAJ017288770 (DE-599)DOAJc75d66948c6d4e8cbdafb711c04d6f4f DE-627 ger DE-627 rakwb eng R5-920 Mozhgan Raigani verfasserin aut Association of MTHFR C677T and MTRR A66G Gene Polymorphisms with Iranian Male Infertility and Its Effect on Seminal Folate and Vitamin B12 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The relation between key enzymes in regulation of folate metabolism and male infertility is the subjectof numerous studies. We aimed to determine whether 5, 10-methylenetetrahydrofolate reductase (MTHFR) C677Tand methionine synthase reductase (MTRR) A66G genotypes are associated with male infertility in Iranian men andto evaluate its effect on seminal levels of folate and vitamin B12.Materials and Methods: In this retrospective study, semen and peripheral blood samples were collected from 254men with oligoasthenoteratozoospermia (OAT) and 77 normozoospermic men who attended Avicenna infertility clinic.Single nucleotide polymorphism (SNP) analysis was carried out in genomic DNA by polymerase chain reaction(PCR)-restriction fragment length polymorphism (RFLP) method for MTHFR C677T and MTRR A66G gene polymorphisms.Results: In MTHFR C677T, our founding showed that T carrier was conversely lower in OAT than normozoospermic men(χ2-test=7.245, P=0.02) whereas in MTRR A66G, A and G carrier showed no significant difference between the two groups(χ2-test=1.079, P=0.53). The concentration of seminal folate was not different between normozoospermic (18.83 ± 17.1 ng/ml) and OAT (16.96 ± 14.2 ng/ml) men (P=0.47). The concentration of vitamin B12 was slightly higher in normozospermicmen (522.6 ± 388.1 pg/ml) compared to OAT men (412.9 ± 303.6 pg/ml, P=0.058).Conclusion: The MTHFR C677T and MTRR A66G have no effect on the concentrations of seminal folate and vitaminB12. The present study showed that two SNPs of MTRR A66G and MTHFR C677T cannot be seen as a risk factor formale factor subfertility. folate male infertility mthfr mtrr vitamin b12 Medicine (General) Niknam Lakpour verfasserin aut Meysam Soleimani verfasserin aut Behrooz Johari verfasserin aut Mohammad Reza Sadeghi verfasserin aut In International Journal of Fertility and Sterility Royan Institute (ACECR), Tehran, 2012 15(2021), 1, Seite 20-25 (DE-627)634381083 (DE-600)2570865-X 20080778 nnns volume:15 year:2021 number:1 pages:20-25 https://doi.org/10.22074/ijfs.2021.6155 kostenfrei https://doaj.org/article/c75d66948c6d4e8cbdafb711c04d6f4f kostenfrei https://www.ijfs.ir/article_46236_dfc22628b49c661aa79230eece8bbf0b.pdf kostenfrei https://doaj.org/toc/2008-076X Journal toc kostenfrei https://doaj.org/toc/2008-0778 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2021 1 20-25
allfields_unstemmed	10.22074/ijfs.2021.6155 doi (DE-627)DOAJ017288770 (DE-599)DOAJc75d66948c6d4e8cbdafb711c04d6f4f DE-627 ger DE-627 rakwb eng R5-920 Mozhgan Raigani verfasserin aut Association of MTHFR C677T and MTRR A66G Gene Polymorphisms with Iranian Male Infertility and Its Effect on Seminal Folate and Vitamin B12 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The relation between key enzymes in regulation of folate metabolism and male infertility is the subjectof numerous studies. We aimed to determine whether 5, 10-methylenetetrahydrofolate reductase (MTHFR) C677Tand methionine synthase reductase (MTRR) A66G genotypes are associated with male infertility in Iranian men andto evaluate its effect on seminal levels of folate and vitamin B12.Materials and Methods: In this retrospective study, semen and peripheral blood samples were collected from 254men with oligoasthenoteratozoospermia (OAT) and 77 normozoospermic men who attended Avicenna infertility clinic.Single nucleotide polymorphism (SNP) analysis was carried out in genomic DNA by polymerase chain reaction(PCR)-restriction fragment length polymorphism (RFLP) method for MTHFR C677T and MTRR A66G gene polymorphisms.Results: In MTHFR C677T, our founding showed that T carrier was conversely lower in OAT than normozoospermic men(χ2-test=7.245, P=0.02) whereas in MTRR A66G, A and G carrier showed no significant difference between the two groups(χ2-test=1.079, P=0.53). The concentration of seminal folate was not different between normozoospermic (18.83 ± 17.1 ng/ml) and OAT (16.96 ± 14.2 ng/ml) men (P=0.47). The concentration of vitamin B12 was slightly higher in normozospermicmen (522.6 ± 388.1 pg/ml) compared to OAT men (412.9 ± 303.6 pg/ml, P=0.058).Conclusion: The MTHFR C677T and MTRR A66G have no effect on the concentrations of seminal folate and vitaminB12. The present study showed that two SNPs of MTRR A66G and MTHFR C677T cannot be seen as a risk factor formale factor subfertility. folate male infertility mthfr mtrr vitamin b12 Medicine (General) Niknam Lakpour verfasserin aut Meysam Soleimani verfasserin aut Behrooz Johari verfasserin aut Mohammad Reza Sadeghi verfasserin aut In International Journal of Fertility and Sterility Royan Institute (ACECR), Tehran, 2012 15(2021), 1, Seite 20-25 (DE-627)634381083 (DE-600)2570865-X 20080778 nnns volume:15 year:2021 number:1 pages:20-25 https://doi.org/10.22074/ijfs.2021.6155 kostenfrei https://doaj.org/article/c75d66948c6d4e8cbdafb711c04d6f4f kostenfrei https://www.ijfs.ir/article_46236_dfc22628b49c661aa79230eece8bbf0b.pdf kostenfrei https://doaj.org/toc/2008-076X Journal toc kostenfrei https://doaj.org/toc/2008-0778 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2021 1 20-25
allfieldsGer	10.22074/ijfs.2021.6155 doi (DE-627)DOAJ017288770 (DE-599)DOAJc75d66948c6d4e8cbdafb711c04d6f4f DE-627 ger DE-627 rakwb eng R5-920 Mozhgan Raigani verfasserin aut Association of MTHFR C677T and MTRR A66G Gene Polymorphisms with Iranian Male Infertility and Its Effect on Seminal Folate and Vitamin B12 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The relation between key enzymes in regulation of folate metabolism and male infertility is the subjectof numerous studies. We aimed to determine whether 5, 10-methylenetetrahydrofolate reductase (MTHFR) C677Tand methionine synthase reductase (MTRR) A66G genotypes are associated with male infertility in Iranian men andto evaluate its effect on seminal levels of folate and vitamin B12.Materials and Methods: In this retrospective study, semen and peripheral blood samples were collected from 254men with oligoasthenoteratozoospermia (OAT) and 77 normozoospermic men who attended Avicenna infertility clinic.Single nucleotide polymorphism (SNP) analysis was carried out in genomic DNA by polymerase chain reaction(PCR)-restriction fragment length polymorphism (RFLP) method for MTHFR C677T and MTRR A66G gene polymorphisms.Results: In MTHFR C677T, our founding showed that T carrier was conversely lower in OAT than normozoospermic men(χ2-test=7.245, P=0.02) whereas in MTRR A66G, A and G carrier showed no significant difference between the two groups(χ2-test=1.079, P=0.53). The concentration of seminal folate was not different between normozoospermic (18.83 ± 17.1 ng/ml) and OAT (16.96 ± 14.2 ng/ml) men (P=0.47). The concentration of vitamin B12 was slightly higher in normozospermicmen (522.6 ± 388.1 pg/ml) compared to OAT men (412.9 ± 303.6 pg/ml, P=0.058).Conclusion: The MTHFR C677T and MTRR A66G have no effect on the concentrations of seminal folate and vitaminB12. The present study showed that two SNPs of MTRR A66G and MTHFR C677T cannot be seen as a risk factor formale factor subfertility. folate male infertility mthfr mtrr vitamin b12 Medicine (General) Niknam Lakpour verfasserin aut Meysam Soleimani verfasserin aut Behrooz Johari verfasserin aut Mohammad Reza Sadeghi verfasserin aut In International Journal of Fertility and Sterility Royan Institute (ACECR), Tehran, 2012 15(2021), 1, Seite 20-25 (DE-627)634381083 (DE-600)2570865-X 20080778 nnns volume:15 year:2021 number:1 pages:20-25 https://doi.org/10.22074/ijfs.2021.6155 kostenfrei https://doaj.org/article/c75d66948c6d4e8cbdafb711c04d6f4f kostenfrei https://www.ijfs.ir/article_46236_dfc22628b49c661aa79230eece8bbf0b.pdf kostenfrei https://doaj.org/toc/2008-076X Journal toc kostenfrei https://doaj.org/toc/2008-0778 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2021 1 20-25
allfieldsSound	10.22074/ijfs.2021.6155 doi (DE-627)DOAJ017288770 (DE-599)DOAJc75d66948c6d4e8cbdafb711c04d6f4f DE-627 ger DE-627 rakwb eng R5-920 Mozhgan Raigani verfasserin aut Association of MTHFR C677T and MTRR A66G Gene Polymorphisms with Iranian Male Infertility and Its Effect on Seminal Folate and Vitamin B12 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The relation between key enzymes in regulation of folate metabolism and male infertility is the subjectof numerous studies. We aimed to determine whether 5, 10-methylenetetrahydrofolate reductase (MTHFR) C677Tand methionine synthase reductase (MTRR) A66G genotypes are associated with male infertility in Iranian men andto evaluate its effect on seminal levels of folate and vitamin B12.Materials and Methods: In this retrospective study, semen and peripheral blood samples were collected from 254men with oligoasthenoteratozoospermia (OAT) and 77 normozoospermic men who attended Avicenna infertility clinic.Single nucleotide polymorphism (SNP) analysis was carried out in genomic DNA by polymerase chain reaction(PCR)-restriction fragment length polymorphism (RFLP) method for MTHFR C677T and MTRR A66G gene polymorphisms.Results: In MTHFR C677T, our founding showed that T carrier was conversely lower in OAT than normozoospermic men(χ2-test=7.245, P=0.02) whereas in MTRR A66G, A and G carrier showed no significant difference between the two groups(χ2-test=1.079, P=0.53). The concentration of seminal folate was not different between normozoospermic (18.83 ± 17.1 ng/ml) and OAT (16.96 ± 14.2 ng/ml) men (P=0.47). The concentration of vitamin B12 was slightly higher in normozospermicmen (522.6 ± 388.1 pg/ml) compared to OAT men (412.9 ± 303.6 pg/ml, P=0.058).Conclusion: The MTHFR C677T and MTRR A66G have no effect on the concentrations of seminal folate and vitaminB12. The present study showed that two SNPs of MTRR A66G and MTHFR C677T cannot be seen as a risk factor formale factor subfertility. folate male infertility mthfr mtrr vitamin b12 Medicine (General) Niknam Lakpour verfasserin aut Meysam Soleimani verfasserin aut Behrooz Johari verfasserin aut Mohammad Reza Sadeghi verfasserin aut In International Journal of Fertility and Sterility Royan Institute (ACECR), Tehran, 2012 15(2021), 1, Seite 20-25 (DE-627)634381083 (DE-600)2570865-X 20080778 nnns volume:15 year:2021 number:1 pages:20-25 https://doi.org/10.22074/ijfs.2021.6155 kostenfrei https://doaj.org/article/c75d66948c6d4e8cbdafb711c04d6f4f kostenfrei https://www.ijfs.ir/article_46236_dfc22628b49c661aa79230eece8bbf0b.pdf kostenfrei https://doaj.org/toc/2008-076X Journal toc kostenfrei https://doaj.org/toc/2008-0778 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2021 1 20-25
language	English
source	In International Journal of Fertility and Sterility 15(2021), 1, Seite 20-25 volume:15 year:2021 number:1 pages:20-25
sourceStr	In International Journal of Fertility and Sterility 15(2021), 1, Seite 20-25 volume:15 year:2021 number:1 pages:20-25
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	folate male infertility mthfr mtrr vitamin b12 Medicine (General)
isfreeaccess_bool	true
container_title	International Journal of Fertility and Sterility
authorswithroles_txt_mv	Mozhgan Raigani @@aut@@ Niknam Lakpour @@aut@@ Meysam Soleimani @@aut@@ Behrooz Johari @@aut@@ Mohammad Reza Sadeghi @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	634381083
id	DOAJ017288770
language_de	englisch
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We aimed to determine whether 5, 10-methylenetetrahydrofolate reductase (MTHFR) C677Tand methionine synthase reductase (MTRR) A66G genotypes are associated with male infertility in Iranian men andto evaluate its effect on seminal levels of folate and vitamin B12.Materials and Methods: In this retrospective study, semen and peripheral blood samples were collected from 254men with oligoasthenoteratozoospermia (OAT) and 77 normozoospermic men who attended Avicenna infertility clinic.Single nucleotide polymorphism (SNP) analysis was carried out in genomic DNA by polymerase chain reaction(PCR)-restriction fragment length polymorphism (RFLP) method for MTHFR C677T and MTRR A66G gene polymorphisms.Results: In MTHFR C677T, our founding showed that T carrier was conversely lower in OAT than normozoospermic men(χ2-test=7.245, P=0.02) whereas in MTRR A66G, A and G carrier showed no significant difference between the two groups(χ2-test=1.079, P=0.53). The concentration of seminal folate was not different between normozoospermic (18.83 ± 17.1 ng/ml) and OAT (16.96 ± 14.2 ng/ml) men (P=0.47). The concentration of vitamin B12 was slightly higher in normozospermicmen (522.6 ± 388.1 pg/ml) compared to OAT men (412.9 ± 303.6 pg/ml, P=0.058).Conclusion: The MTHFR C677T and MTRR A66G have no effect on the concentrations of seminal folate and vitaminB12. 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callnumber-first	R - Medicine
author	Mozhgan Raigani
spellingShingle	Mozhgan Raigani misc R5-920 misc folate misc male infertility misc mthfr misc mtrr misc vitamin b12 misc Medicine (General) Association of MTHFR C677T and MTRR A66G Gene Polymorphisms with Iranian Male Infertility and Its Effect on Seminal Folate and Vitamin B12
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topic_title	R5-920 Association of MTHFR C677T and MTRR A66G Gene Polymorphisms with Iranian Male Infertility and Its Effect on Seminal Folate and Vitamin B12 folate male infertility mthfr mtrr vitamin b12
topic	misc R5-920 misc folate misc male infertility misc mthfr misc mtrr misc vitamin b12 misc Medicine (General)
topic_unstemmed	misc R5-920 misc folate misc male infertility misc mthfr misc mtrr misc vitamin b12 misc Medicine (General)
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title	Association of MTHFR C677T and MTRR A66G Gene Polymorphisms with Iranian Male Infertility and Its Effect on Seminal Folate and Vitamin B12
ctrlnum	(DE-627)DOAJ017288770 (DE-599)DOAJc75d66948c6d4e8cbdafb711c04d6f4f
title_full	Association of MTHFR C677T and MTRR A66G Gene Polymorphisms with Iranian Male Infertility and Its Effect on Seminal Folate and Vitamin B12
author_sort	Mozhgan Raigani
journal	International Journal of Fertility and Sterility
journalStr	International Journal of Fertility and Sterility
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author_browse	Mozhgan Raigani Niknam Lakpour Meysam Soleimani Behrooz Johari Mohammad Reza Sadeghi
container_volume	15
class	R5-920
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author-letter	Mozhgan Raigani
doi_str_mv	10.22074/ijfs.2021.6155
author2-role	verfasserin
title_sort	association of mthfr c677t and mtrr a66g gene polymorphisms with iranian male infertility and its effect on seminal folate and vitamin b12
callnumber	R5-920
title_auth	Association of MTHFR C677T and MTRR A66G Gene Polymorphisms with Iranian Male Infertility and Its Effect on Seminal Folate and Vitamin B12
abstract	Background: The relation between key enzymes in regulation of folate metabolism and male infertility is the subjectof numerous studies. We aimed to determine whether 5, 10-methylenetetrahydrofolate reductase (MTHFR) C677Tand methionine synthase reductase (MTRR) A66G genotypes are associated with male infertility in Iranian men andto evaluate its effect on seminal levels of folate and vitamin B12.Materials and Methods: In this retrospective study, semen and peripheral blood samples were collected from 254men with oligoasthenoteratozoospermia (OAT) and 77 normozoospermic men who attended Avicenna infertility clinic.Single nucleotide polymorphism (SNP) analysis was carried out in genomic DNA by polymerase chain reaction(PCR)-restriction fragment length polymorphism (RFLP) method for MTHFR C677T and MTRR A66G gene polymorphisms.Results: In MTHFR C677T, our founding showed that T carrier was conversely lower in OAT than normozoospermic men(χ2-test=7.245, P=0.02) whereas in MTRR A66G, A and G carrier showed no significant difference between the two groups(χ2-test=1.079, P=0.53). The concentration of seminal folate was not different between normozoospermic (18.83 ± 17.1 ng/ml) and OAT (16.96 ± 14.2 ng/ml) men (P=0.47). The concentration of vitamin B12 was slightly higher in normozospermicmen (522.6 ± 388.1 pg/ml) compared to OAT men (412.9 ± 303.6 pg/ml, P=0.058).Conclusion: The MTHFR C677T and MTRR A66G have no effect on the concentrations of seminal folate and vitaminB12. The present study showed that two SNPs of MTRR A66G and MTHFR C677T cannot be seen as a risk factor formale factor subfertility.
abstractGer	Background: The relation between key enzymes in regulation of folate metabolism and male infertility is the subjectof numerous studies. We aimed to determine whether 5, 10-methylenetetrahydrofolate reductase (MTHFR) C677Tand methionine synthase reductase (MTRR) A66G genotypes are associated with male infertility in Iranian men andto evaluate its effect on seminal levels of folate and vitamin B12.Materials and Methods: In this retrospective study, semen and peripheral blood samples were collected from 254men with oligoasthenoteratozoospermia (OAT) and 77 normozoospermic men who attended Avicenna infertility clinic.Single nucleotide polymorphism (SNP) analysis was carried out in genomic DNA by polymerase chain reaction(PCR)-restriction fragment length polymorphism (RFLP) method for MTHFR C677T and MTRR A66G gene polymorphisms.Results: In MTHFR C677T, our founding showed that T carrier was conversely lower in OAT than normozoospermic men(χ2-test=7.245, P=0.02) whereas in MTRR A66G, A and G carrier showed no significant difference between the two groups(χ2-test=1.079, P=0.53). The concentration of seminal folate was not different between normozoospermic (18.83 ± 17.1 ng/ml) and OAT (16.96 ± 14.2 ng/ml) men (P=0.47). The concentration of vitamin B12 was slightly higher in normozospermicmen (522.6 ± 388.1 pg/ml) compared to OAT men (412.9 ± 303.6 pg/ml, P=0.058).Conclusion: The MTHFR C677T and MTRR A66G have no effect on the concentrations of seminal folate and vitaminB12. The present study showed that two SNPs of MTRR A66G and MTHFR C677T cannot be seen as a risk factor formale factor subfertility.
abstract_unstemmed	Background: The relation between key enzymes in regulation of folate metabolism and male infertility is the subjectof numerous studies. We aimed to determine whether 5, 10-methylenetetrahydrofolate reductase (MTHFR) C677Tand methionine synthase reductase (MTRR) A66G genotypes are associated with male infertility in Iranian men andto evaluate its effect on seminal levels of folate and vitamin B12.Materials and Methods: In this retrospective study, semen and peripheral blood samples were collected from 254men with oligoasthenoteratozoospermia (OAT) and 77 normozoospermic men who attended Avicenna infertility clinic.Single nucleotide polymorphism (SNP) analysis was carried out in genomic DNA by polymerase chain reaction(PCR)-restriction fragment length polymorphism (RFLP) method for MTHFR C677T and MTRR A66G gene polymorphisms.Results: In MTHFR C677T, our founding showed that T carrier was conversely lower in OAT than normozoospermic men(χ2-test=7.245, P=0.02) whereas in MTRR A66G, A and G carrier showed no significant difference between the two groups(χ2-test=1.079, P=0.53). The concentration of seminal folate was not different between normozoospermic (18.83 ± 17.1 ng/ml) and OAT (16.96 ± 14.2 ng/ml) men (P=0.47). The concentration of vitamin B12 was slightly higher in normozospermicmen (522.6 ± 388.1 pg/ml) compared to OAT men (412.9 ± 303.6 pg/ml, P=0.058).Conclusion: The MTHFR C677T and MTRR A66G have no effect on the concentrations of seminal folate and vitaminB12. The present study showed that two SNPs of MTRR A66G and MTHFR C677T cannot be seen as a risk factor formale factor subfertility.
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container_issue	1
title_short	Association of MTHFR C677T and MTRR A66G Gene Polymorphisms with Iranian Male Infertility and Its Effect on Seminal Folate and Vitamin B12
url	https://doi.org/10.22074/ijfs.2021.6155 https://doaj.org/article/c75d66948c6d4e8cbdafb711c04d6f4f https://www.ijfs.ir/article_46236_dfc22628b49c661aa79230eece8bbf0b.pdf https://doaj.org/toc/2008-076X https://doaj.org/toc/2008-0778
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author2	Niknam Lakpour Meysam Soleimani Behrooz Johari Mohammad Reza Sadeghi
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up_date	2024-07-04T01:02:14.638Z
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Association of MTHFR C677T and MTRR A66G Gene Polymorphisms with Iranian Male Infertility and Its Effect on Seminal Folate and Vitamin B12

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