Interaction of Proteins Identified in Human Thyroid Cells

Influence of gravity forces on the regulation of protein expression by healthy and malignant thyroid cells was studied with the aim to identify protein interactions. Western blot analyses of a limited number of proteins suggested a time-dependent regulation of protein expression by simulated microgr...
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Gespeichert in:

Autor*in:	Jessica Pietsch [verfasserIn] Stefan Riwaldt [verfasserIn] Johann Bauer [verfasserIn] Albert Sickmann [verfasserIn] Gerhard Weber [verfasserIn] Jirka Grosse [verfasserIn] Manfred Infanger [verfasserIn] Christoph Eilles [verfasserIn] Daniela Grimm [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2013

Schlagwörter:	thyroid cancer cell line FTC-133 thyroid cancer cell line CGTH W-1 normal thyrocyte cells HTU-5 Random Positioning Machine (RPM) STRING network analysis

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 14(2013), 1, Seite 1164-1178
Übergeordnetes Werk:	volume:14 ; year:2013 ; number:1 ; pages:1164-1178

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/ijms14011164

Katalog-ID:	DOAJ017420962

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author	Jessica Pietsch
spellingShingle	Jessica Pietsch misc QH301-705.5 misc QD1-999 misc thyroid cancer cell line FTC-133 misc thyroid cancer cell line CGTH W-1 misc normal thyrocyte cells HTU-5 misc Random Positioning Machine (RPM) misc STRING network analysis misc Biology (General) misc Chemistry Interaction of Proteins Identified in Human Thyroid Cells
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topic_title	QH301-705.5 QD1-999 Interaction of Proteins Identified in Human Thyroid Cells thyroid cancer cell line FTC-133 thyroid cancer cell line CGTH W-1 normal thyrocyte cells HTU-5 Random Positioning Machine (RPM) STRING network analysis
topic	misc QH301-705.5 misc QD1-999 misc thyroid cancer cell line FTC-133 misc thyroid cancer cell line CGTH W-1 misc normal thyrocyte cells HTU-5 misc Random Positioning Machine (RPM) misc STRING network analysis misc Biology (General) misc Chemistry
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title_auth	Interaction of Proteins Identified in Human Thyroid Cells
abstract	Influence of gravity forces on the regulation of protein expression by healthy and malignant thyroid cells was studied with the aim to identify protein interactions. Western blot analyses of a limited number of proteins suggested a time-dependent regulation of protein expression by simulated microgravity. After applying free flow isoelectric focusing and mass spectrometry to search for differently expressed proteins by thyroid cells exposed to simulated microgravity for three days, a considerable number of candidates for gravi-sensitive proteins were detected. In order to show how proteins sensitive to microgravity could directly influence other proteins, we investigated all polypeptide chains identified with Mascot scores above 100, looking for groups of interacting proteins. Hence, UniProtKB entry numbers of all detected proteins were entered into the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) and processed. The program indicated that we had detected various groups of interacting proteins in each of the three cell lines studied. The major groups of interacting proteins play a role in pathways of carbohydrate and protein metabolism, regulation of cell growth and cell membrane structuring. Analyzing these groups, networks of interaction could be established which show how a punctual influence of simulated microgravity may propagate via various members of interaction chains.
abstractGer	Influence of gravity forces on the regulation of protein expression by healthy and malignant thyroid cells was studied with the aim to identify protein interactions. Western blot analyses of a limited number of proteins suggested a time-dependent regulation of protein expression by simulated microgravity. After applying free flow isoelectric focusing and mass spectrometry to search for differently expressed proteins by thyroid cells exposed to simulated microgravity for three days, a considerable number of candidates for gravi-sensitive proteins were detected. In order to show how proteins sensitive to microgravity could directly influence other proteins, we investigated all polypeptide chains identified with Mascot scores above 100, looking for groups of interacting proteins. Hence, UniProtKB entry numbers of all detected proteins were entered into the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) and processed. The program indicated that we had detected various groups of interacting proteins in each of the three cell lines studied. The major groups of interacting proteins play a role in pathways of carbohydrate and protein metabolism, regulation of cell growth and cell membrane structuring. Analyzing these groups, networks of interaction could be established which show how a punctual influence of simulated microgravity may propagate via various members of interaction chains.
abstract_unstemmed	Influence of gravity forces on the regulation of protein expression by healthy and malignant thyroid cells was studied with the aim to identify protein interactions. Western blot analyses of a limited number of proteins suggested a time-dependent regulation of protein expression by simulated microgravity. After applying free flow isoelectric focusing and mass spectrometry to search for differently expressed proteins by thyroid cells exposed to simulated microgravity for three days, a considerable number of candidates for gravi-sensitive proteins were detected. In order to show how proteins sensitive to microgravity could directly influence other proteins, we investigated all polypeptide chains identified with Mascot scores above 100, looking for groups of interacting proteins. Hence, UniProtKB entry numbers of all detected proteins were entered into the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) and processed. The program indicated that we had detected various groups of interacting proteins in each of the three cell lines studied. The major groups of interacting proteins play a role in pathways of carbohydrate and protein metabolism, regulation of cell growth and cell membrane structuring. Analyzing these groups, networks of interaction could be established which show how a punctual influence of simulated microgravity may propagate via various members of interaction chains.
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title_short	Interaction of Proteins Identified in Human Thyroid Cells
url	https://doi.org/10.3390/ijms14011164 https://doaj.org/article/d5d0a5baa6714260b9e8ed0544641008 http://www.mdpi.com/1422-0067/14/1/1164 https://doaj.org/toc/1422-0067
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