Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2

MicroRNAs play key roles during ovary development, with emerging evidence suggesting that miR-202-5p is specifically expressed in female animal gonads. Granulosa cells (GCs) are somatic cells that are closely related to the development of female gametes in mammalian ovaries. However, the biological...
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Autor*in:	Qiang Ding [verfasserIn] Miaohan Jin [verfasserIn] Yaoyue Wang [verfasserIn] Jiao Liu [verfasserIn] Peter Kalds [verfasserIn] Ying Wang [verfasserIn] Yuxin Yang [verfasserIn] Xiaolong Wang [verfasserIn] Yulin Chen [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	mir-202-5p tgf-β signaling pathway goat granulosa cell cell apoptosis sf1

Übergeordnetes Werk:	In: Cells - MDPI AG, 2012, 9(2020), 2, p 445
Übergeordnetes Werk:	volume:9 ; year:2020 ; number:2, p 445

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/cells9020445

Katalog-ID:	DOAJ017436001

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245	1	0	\|a Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2
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520			\|a MicroRNAs play key roles during ovary development, with emerging evidence suggesting that miR-202-5p is specifically expressed in female animal gonads. Granulosa cells (GCs) are somatic cells that are closely related to the development of female gametes in mammalian ovaries. However, the biological roles of miR-202-5p in GCs remain unknown. Here, we show that miR-202-5p is specifically expressed in GCs and accumulates in extracellular vesicles (EVs) from large growth follicles in goat ovaries. In vitro assays showed that miR-202-5p induced apoptosis and suppressed the proliferation of goat GCs. We further revealed that miR-202-5p is a functional miRNA that targets the transforming growth factor-beta type II receptor (<i<TGFβR2</i<). MiR-202-5p attenuated TGF-β/SMAD signaling through the degradation of TGFβR2 at both the mRNA and protein level, decreasing p-SMAD3 levels in GCs. Moreover, we verified that steroidogenic factor 1 (SF1) is a transcriptional factor that binds to the promoters of miR-202 and cytochrome P450 family 19 subfamily A member 1 (<i<CYP19A1</i<) through luciferase reporter and chromatin immunoprecipitation (ChIP) assays. That contributed to positive correlation between miR-202-5p and <i<CYP19A1</i< expression and estradiol (E2) release. Furthermore, SF1 repressed TGFβR2 and p-SMAD3 levels in GCs through the transactivation of miR-202-5p. Taken together, these results suggest a mechanism by which miR-202-5p regulates canonical TGF-β/SMAD signaling through targeting <i<TGFβR2</i< in GCs. This provides insight into the transcriptional regulation of <i<miR-202</i< and <i<CYP19A1</i< during goat ovarian follicular development.
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author_variant	q d qd m j mj y w yw j l jl p k pk y w yw y y yy x w xw y c yc
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allfields	10.3390/cells9020445 doi (DE-627)DOAJ017436001 (DE-599)DOAJe290060e6d8c422ba874670c6b6de2bd DE-627 ger DE-627 rakwb eng QH301-705.5 Qiang Ding verfasserin aut Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier MicroRNAs play key roles during ovary development, with emerging evidence suggesting that miR-202-5p is specifically expressed in female animal gonads. Granulosa cells (GCs) are somatic cells that are closely related to the development of female gametes in mammalian ovaries. However, the biological roles of miR-202-5p in GCs remain unknown. Here, we show that miR-202-5p is specifically expressed in GCs and accumulates in extracellular vesicles (EVs) from large growth follicles in goat ovaries. In vitro assays showed that miR-202-5p induced apoptosis and suppressed the proliferation of goat GCs. We further revealed that miR-202-5p is a functional miRNA that targets the transforming growth factor-beta type II receptor (<i<TGFβR2</i<). MiR-202-5p attenuated TGF-β/SMAD signaling through the degradation of TGFβR2 at both the mRNA and protein level, decreasing p-SMAD3 levels in GCs. Moreover, we verified that steroidogenic factor 1 (SF1) is a transcriptional factor that binds to the promoters of miR-202 and cytochrome P450 family 19 subfamily A member 1 (<i<CYP19A1</i<) through luciferase reporter and chromatin immunoprecipitation (ChIP) assays. That contributed to positive correlation between miR-202-5p and <i<CYP19A1</i< expression and estradiol (E2) release. Furthermore, SF1 repressed TGFβR2 and p-SMAD3 levels in GCs through the transactivation of miR-202-5p. Taken together, these results suggest a mechanism by which miR-202-5p regulates canonical TGF-β/SMAD signaling through targeting <i<TGFβR2</i< in GCs. This provides insight into the transcriptional regulation of <i<miR-202</i< and <i<CYP19A1</i< during goat ovarian follicular development. mir-202-5p <i<tgfβr2</i< tgf-β signaling pathway goat granulosa cell cell apoptosis sf1 Biology (General) Miaohan Jin verfasserin aut Yaoyue Wang verfasserin aut Jiao Liu verfasserin aut Peter Kalds verfasserin aut Ying Wang verfasserin aut Yuxin Yang verfasserin aut Xiaolong Wang verfasserin aut Yulin Chen verfasserin aut In Cells MDPI AG, 2012 9(2020), 2, p 445 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:9 year:2020 number:2, p 445 https://doi.org/10.3390/cells9020445 kostenfrei https://doaj.org/article/e290060e6d8c422ba874670c6b6de2bd kostenfrei https://www.mdpi.com/2073-4409/9/2/445 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 2, p 445
spelling	10.3390/cells9020445 doi (DE-627)DOAJ017436001 (DE-599)DOAJe290060e6d8c422ba874670c6b6de2bd DE-627 ger DE-627 rakwb eng QH301-705.5 Qiang Ding verfasserin aut Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier MicroRNAs play key roles during ovary development, with emerging evidence suggesting that miR-202-5p is specifically expressed in female animal gonads. Granulosa cells (GCs) are somatic cells that are closely related to the development of female gametes in mammalian ovaries. However, the biological roles of miR-202-5p in GCs remain unknown. Here, we show that miR-202-5p is specifically expressed in GCs and accumulates in extracellular vesicles (EVs) from large growth follicles in goat ovaries. In vitro assays showed that miR-202-5p induced apoptosis and suppressed the proliferation of goat GCs. We further revealed that miR-202-5p is a functional miRNA that targets the transforming growth factor-beta type II receptor (<i<TGFβR2</i<). MiR-202-5p attenuated TGF-β/SMAD signaling through the degradation of TGFβR2 at both the mRNA and protein level, decreasing p-SMAD3 levels in GCs. Moreover, we verified that steroidogenic factor 1 (SF1) is a transcriptional factor that binds to the promoters of miR-202 and cytochrome P450 family 19 subfamily A member 1 (<i<CYP19A1</i<) through luciferase reporter and chromatin immunoprecipitation (ChIP) assays. That contributed to positive correlation between miR-202-5p and <i<CYP19A1</i< expression and estradiol (E2) release. Furthermore, SF1 repressed TGFβR2 and p-SMAD3 levels in GCs through the transactivation of miR-202-5p. Taken together, these results suggest a mechanism by which miR-202-5p regulates canonical TGF-β/SMAD signaling through targeting <i<TGFβR2</i< in GCs. This provides insight into the transcriptional regulation of <i<miR-202</i< and <i<CYP19A1</i< during goat ovarian follicular development. mir-202-5p <i<tgfβr2</i< tgf-β signaling pathway goat granulosa cell cell apoptosis sf1 Biology (General) Miaohan Jin verfasserin aut Yaoyue Wang verfasserin aut Jiao Liu verfasserin aut Peter Kalds verfasserin aut Ying Wang verfasserin aut Yuxin Yang verfasserin aut Xiaolong Wang verfasserin aut Yulin Chen verfasserin aut In Cells MDPI AG, 2012 9(2020), 2, p 445 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:9 year:2020 number:2, p 445 https://doi.org/10.3390/cells9020445 kostenfrei https://doaj.org/article/e290060e6d8c422ba874670c6b6de2bd kostenfrei https://www.mdpi.com/2073-4409/9/2/445 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 2, p 445
allfields_unstemmed	10.3390/cells9020445 doi (DE-627)DOAJ017436001 (DE-599)DOAJe290060e6d8c422ba874670c6b6de2bd DE-627 ger DE-627 rakwb eng QH301-705.5 Qiang Ding verfasserin aut Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier MicroRNAs play key roles during ovary development, with emerging evidence suggesting that miR-202-5p is specifically expressed in female animal gonads. Granulosa cells (GCs) are somatic cells that are closely related to the development of female gametes in mammalian ovaries. However, the biological roles of miR-202-5p in GCs remain unknown. Here, we show that miR-202-5p is specifically expressed in GCs and accumulates in extracellular vesicles (EVs) from large growth follicles in goat ovaries. In vitro assays showed that miR-202-5p induced apoptosis and suppressed the proliferation of goat GCs. We further revealed that miR-202-5p is a functional miRNA that targets the transforming growth factor-beta type II receptor (<i<TGFβR2</i<). MiR-202-5p attenuated TGF-β/SMAD signaling through the degradation of TGFβR2 at both the mRNA and protein level, decreasing p-SMAD3 levels in GCs. Moreover, we verified that steroidogenic factor 1 (SF1) is a transcriptional factor that binds to the promoters of miR-202 and cytochrome P450 family 19 subfamily A member 1 (<i<CYP19A1</i<) through luciferase reporter and chromatin immunoprecipitation (ChIP) assays. That contributed to positive correlation between miR-202-5p and <i<CYP19A1</i< expression and estradiol (E2) release. Furthermore, SF1 repressed TGFβR2 and p-SMAD3 levels in GCs through the transactivation of miR-202-5p. Taken together, these results suggest a mechanism by which miR-202-5p regulates canonical TGF-β/SMAD signaling through targeting <i<TGFβR2</i< in GCs. This provides insight into the transcriptional regulation of <i<miR-202</i< and <i<CYP19A1</i< during goat ovarian follicular development. mir-202-5p <i<tgfβr2</i< tgf-β signaling pathway goat granulosa cell cell apoptosis sf1 Biology (General) Miaohan Jin verfasserin aut Yaoyue Wang verfasserin aut Jiao Liu verfasserin aut Peter Kalds verfasserin aut Ying Wang verfasserin aut Yuxin Yang verfasserin aut Xiaolong Wang verfasserin aut Yulin Chen verfasserin aut In Cells MDPI AG, 2012 9(2020), 2, p 445 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:9 year:2020 number:2, p 445 https://doi.org/10.3390/cells9020445 kostenfrei https://doaj.org/article/e290060e6d8c422ba874670c6b6de2bd kostenfrei https://www.mdpi.com/2073-4409/9/2/445 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 2, p 445
allfieldsGer	10.3390/cells9020445 doi (DE-627)DOAJ017436001 (DE-599)DOAJe290060e6d8c422ba874670c6b6de2bd DE-627 ger DE-627 rakwb eng QH301-705.5 Qiang Ding verfasserin aut Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier MicroRNAs play key roles during ovary development, with emerging evidence suggesting that miR-202-5p is specifically expressed in female animal gonads. Granulosa cells (GCs) are somatic cells that are closely related to the development of female gametes in mammalian ovaries. However, the biological roles of miR-202-5p in GCs remain unknown. Here, we show that miR-202-5p is specifically expressed in GCs and accumulates in extracellular vesicles (EVs) from large growth follicles in goat ovaries. In vitro assays showed that miR-202-5p induced apoptosis and suppressed the proliferation of goat GCs. We further revealed that miR-202-5p is a functional miRNA that targets the transforming growth factor-beta type II receptor (<i<TGFβR2</i<). MiR-202-5p attenuated TGF-β/SMAD signaling through the degradation of TGFβR2 at both the mRNA and protein level, decreasing p-SMAD3 levels in GCs. Moreover, we verified that steroidogenic factor 1 (SF1) is a transcriptional factor that binds to the promoters of miR-202 and cytochrome P450 family 19 subfamily A member 1 (<i<CYP19A1</i<) through luciferase reporter and chromatin immunoprecipitation (ChIP) assays. That contributed to positive correlation between miR-202-5p and <i<CYP19A1</i< expression and estradiol (E2) release. Furthermore, SF1 repressed TGFβR2 and p-SMAD3 levels in GCs through the transactivation of miR-202-5p. Taken together, these results suggest a mechanism by which miR-202-5p regulates canonical TGF-β/SMAD signaling through targeting <i<TGFβR2</i< in GCs. This provides insight into the transcriptional regulation of <i<miR-202</i< and <i<CYP19A1</i< during goat ovarian follicular development. mir-202-5p <i<tgfβr2</i< tgf-β signaling pathway goat granulosa cell cell apoptosis sf1 Biology (General) Miaohan Jin verfasserin aut Yaoyue Wang verfasserin aut Jiao Liu verfasserin aut Peter Kalds verfasserin aut Ying Wang verfasserin aut Yuxin Yang verfasserin aut Xiaolong Wang verfasserin aut Yulin Chen verfasserin aut In Cells MDPI AG, 2012 9(2020), 2, p 445 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:9 year:2020 number:2, p 445 https://doi.org/10.3390/cells9020445 kostenfrei https://doaj.org/article/e290060e6d8c422ba874670c6b6de2bd kostenfrei https://www.mdpi.com/2073-4409/9/2/445 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 2, p 445
allfieldsSound	10.3390/cells9020445 doi (DE-627)DOAJ017436001 (DE-599)DOAJe290060e6d8c422ba874670c6b6de2bd DE-627 ger DE-627 rakwb eng QH301-705.5 Qiang Ding verfasserin aut Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier MicroRNAs play key roles during ovary development, with emerging evidence suggesting that miR-202-5p is specifically expressed in female animal gonads. Granulosa cells (GCs) are somatic cells that are closely related to the development of female gametes in mammalian ovaries. However, the biological roles of miR-202-5p in GCs remain unknown. Here, we show that miR-202-5p is specifically expressed in GCs and accumulates in extracellular vesicles (EVs) from large growth follicles in goat ovaries. In vitro assays showed that miR-202-5p induced apoptosis and suppressed the proliferation of goat GCs. We further revealed that miR-202-5p is a functional miRNA that targets the transforming growth factor-beta type II receptor (<i<TGFβR2</i<). MiR-202-5p attenuated TGF-β/SMAD signaling through the degradation of TGFβR2 at both the mRNA and protein level, decreasing p-SMAD3 levels in GCs. Moreover, we verified that steroidogenic factor 1 (SF1) is a transcriptional factor that binds to the promoters of miR-202 and cytochrome P450 family 19 subfamily A member 1 (<i<CYP19A1</i<) through luciferase reporter and chromatin immunoprecipitation (ChIP) assays. That contributed to positive correlation between miR-202-5p and <i<CYP19A1</i< expression and estradiol (E2) release. Furthermore, SF1 repressed TGFβR2 and p-SMAD3 levels in GCs through the transactivation of miR-202-5p. Taken together, these results suggest a mechanism by which miR-202-5p regulates canonical TGF-β/SMAD signaling through targeting <i<TGFβR2</i< in GCs. This provides insight into the transcriptional regulation of <i<miR-202</i< and <i<CYP19A1</i< during goat ovarian follicular development. mir-202-5p <i<tgfβr2</i< tgf-β signaling pathway goat granulosa cell cell apoptosis sf1 Biology (General) Miaohan Jin verfasserin aut Yaoyue Wang verfasserin aut Jiao Liu verfasserin aut Peter Kalds verfasserin aut Ying Wang verfasserin aut Yuxin Yang verfasserin aut Xiaolong Wang verfasserin aut Yulin Chen verfasserin aut In Cells MDPI AG, 2012 9(2020), 2, p 445 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:9 year:2020 number:2, p 445 https://doi.org/10.3390/cells9020445 kostenfrei https://doaj.org/article/e290060e6d8c422ba874670c6b6de2bd kostenfrei https://www.mdpi.com/2073-4409/9/2/445 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 2, p 445
language	English
source	In Cells 9(2020), 2, p 445 volume:9 year:2020 number:2, p 445
sourceStr	In Cells 9(2020), 2, p 445 volume:9 year:2020 number:2, p 445
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	mir-202-5p <i<tgfβr2</i< tgf-β signaling pathway goat granulosa cell cell apoptosis sf1 Biology (General)
isfreeaccess_bool	true
container_title	Cells
authorswithroles_txt_mv	Qiang Ding @@aut@@ Miaohan Jin @@aut@@ Yaoyue Wang @@aut@@ Jiao Liu @@aut@@ Peter Kalds @@aut@@ Ying Wang @@aut@@ Yuxin Yang @@aut@@ Xiaolong Wang @@aut@@ Yulin Chen @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
hierarchy_top_id	718622081
id	DOAJ017436001
language_de	englisch
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callnumber-first	Q - Science
author	Qiang Ding
spellingShingle	Qiang Ding misc QH301-705.5 misc mir-202-5p misc <i<tgfβr2</i< misc tgf-β signaling pathway misc goat granulosa cell misc cell apoptosis misc sf1 misc Biology (General) Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2
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topic_title	QH301-705.5 Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2 mir-202-5p <i<tgfβr2</i< tgf-β signaling pathway goat granulosa cell cell apoptosis sf1
topic	misc QH301-705.5 misc mir-202-5p misc <i<tgfβr2</i< misc tgf-β signaling pathway misc goat granulosa cell misc cell apoptosis misc sf1 misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc mir-202-5p misc <i<tgfβr2</i< misc tgf-β signaling pathway misc goat granulosa cell misc cell apoptosis misc sf1 misc Biology (General)
topic_browse	misc QH301-705.5 misc mir-202-5p misc <i<tgfβr2</i< misc tgf-β signaling pathway misc goat granulosa cell misc cell apoptosis misc sf1 misc Biology (General)
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
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title	Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2
ctrlnum	(DE-627)DOAJ017436001 (DE-599)DOAJe290060e6d8c422ba874670c6b6de2bd
title_full	Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2
author_sort	Qiang Ding
journal	Cells
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author_browse	Qiang Ding Miaohan Jin Yaoyue Wang Jiao Liu Peter Kalds Ying Wang Yuxin Yang Xiaolong Wang Yulin Chen
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author-letter	Qiang Ding
doi_str_mv	10.3390/cells9020445
author2-role	verfasserin
title_sort	transactivation of mir-202-5p by steroidogenic factor 1 (sf1) induces apoptosis in goat granulosa cells by targeting tgfβr2
callnumber	QH301-705.5
title_auth	Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2
abstract	MicroRNAs play key roles during ovary development, with emerging evidence suggesting that miR-202-5p is specifically expressed in female animal gonads. Granulosa cells (GCs) are somatic cells that are closely related to the development of female gametes in mammalian ovaries. However, the biological roles of miR-202-5p in GCs remain unknown. Here, we show that miR-202-5p is specifically expressed in GCs and accumulates in extracellular vesicles (EVs) from large growth follicles in goat ovaries. In vitro assays showed that miR-202-5p induced apoptosis and suppressed the proliferation of goat GCs. We further revealed that miR-202-5p is a functional miRNA that targets the transforming growth factor-beta type II receptor (<i<TGFβR2</i<). MiR-202-5p attenuated TGF-β/SMAD signaling through the degradation of TGFβR2 at both the mRNA and protein level, decreasing p-SMAD3 levels in GCs. Moreover, we verified that steroidogenic factor 1 (SF1) is a transcriptional factor that binds to the promoters of miR-202 and cytochrome P450 family 19 subfamily A member 1 (<i<CYP19A1</i<) through luciferase reporter and chromatin immunoprecipitation (ChIP) assays. That contributed to positive correlation between miR-202-5p and <i<CYP19A1</i< expression and estradiol (E2) release. Furthermore, SF1 repressed TGFβR2 and p-SMAD3 levels in GCs through the transactivation of miR-202-5p. Taken together, these results suggest a mechanism by which miR-202-5p regulates canonical TGF-β/SMAD signaling through targeting <i<TGFβR2</i< in GCs. This provides insight into the transcriptional regulation of <i<miR-202</i< and <i<CYP19A1</i< during goat ovarian follicular development.
abstractGer	MicroRNAs play key roles during ovary development, with emerging evidence suggesting that miR-202-5p is specifically expressed in female animal gonads. Granulosa cells (GCs) are somatic cells that are closely related to the development of female gametes in mammalian ovaries. However, the biological roles of miR-202-5p in GCs remain unknown. Here, we show that miR-202-5p is specifically expressed in GCs and accumulates in extracellular vesicles (EVs) from large growth follicles in goat ovaries. In vitro assays showed that miR-202-5p induced apoptosis and suppressed the proliferation of goat GCs. We further revealed that miR-202-5p is a functional miRNA that targets the transforming growth factor-beta type II receptor (<i<TGFβR2</i<). MiR-202-5p attenuated TGF-β/SMAD signaling through the degradation of TGFβR2 at both the mRNA and protein level, decreasing p-SMAD3 levels in GCs. Moreover, we verified that steroidogenic factor 1 (SF1) is a transcriptional factor that binds to the promoters of miR-202 and cytochrome P450 family 19 subfamily A member 1 (<i<CYP19A1</i<) through luciferase reporter and chromatin immunoprecipitation (ChIP) assays. That contributed to positive correlation between miR-202-5p and <i<CYP19A1</i< expression and estradiol (E2) release. Furthermore, SF1 repressed TGFβR2 and p-SMAD3 levels in GCs through the transactivation of miR-202-5p. Taken together, these results suggest a mechanism by which miR-202-5p regulates canonical TGF-β/SMAD signaling through targeting <i<TGFβR2</i< in GCs. This provides insight into the transcriptional regulation of <i<miR-202</i< and <i<CYP19A1</i< during goat ovarian follicular development.
abstract_unstemmed	MicroRNAs play key roles during ovary development, with emerging evidence suggesting that miR-202-5p is specifically expressed in female animal gonads. Granulosa cells (GCs) are somatic cells that are closely related to the development of female gametes in mammalian ovaries. However, the biological roles of miR-202-5p in GCs remain unknown. Here, we show that miR-202-5p is specifically expressed in GCs and accumulates in extracellular vesicles (EVs) from large growth follicles in goat ovaries. In vitro assays showed that miR-202-5p induced apoptosis and suppressed the proliferation of goat GCs. We further revealed that miR-202-5p is a functional miRNA that targets the transforming growth factor-beta type II receptor (<i<TGFβR2</i<). MiR-202-5p attenuated TGF-β/SMAD signaling through the degradation of TGFβR2 at both the mRNA and protein level, decreasing p-SMAD3 levels in GCs. Moreover, we verified that steroidogenic factor 1 (SF1) is a transcriptional factor that binds to the promoters of miR-202 and cytochrome P450 family 19 subfamily A member 1 (<i<CYP19A1</i<) through luciferase reporter and chromatin immunoprecipitation (ChIP) assays. That contributed to positive correlation between miR-202-5p and <i<CYP19A1</i< expression and estradiol (E2) release. Furthermore, SF1 repressed TGFβR2 and p-SMAD3 levels in GCs through the transactivation of miR-202-5p. Taken together, these results suggest a mechanism by which miR-202-5p regulates canonical TGF-β/SMAD signaling through targeting <i<TGFβR2</i< in GCs. This provides insight into the transcriptional regulation of <i<miR-202</i< and <i<CYP19A1</i< during goat ovarian follicular development.
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container_issue	2, p 445
title_short	Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2
url	https://doi.org/10.3390/cells9020445 https://doaj.org/article/e290060e6d8c422ba874670c6b6de2bd https://www.mdpi.com/2073-4409/9/2/445 https://doaj.org/toc/2073-4409
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author2	Miaohan Jin Yaoyue Wang Jiao Liu Peter Kalds Ying Wang Yuxin Yang Xiaolong Wang Yulin Chen
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up_date	2024-07-04T01:35:47.065Z
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Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2

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