Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2
MicroRNAs play key roles during ovary development, with emerging evidence suggesting that miR-202-5p is specifically expressed in female animal gonads. Granulosa cells (GCs) are somatic cells that are closely related to the development of female gametes in mammalian ovaries. However, the biological...
Ausführliche Beschreibung
Autor*in: |
Qiang Ding [verfasserIn] Miaohan Jin [verfasserIn] Yaoyue Wang [verfasserIn] Jiao Liu [verfasserIn] Peter Kalds [verfasserIn] Ying Wang [verfasserIn] Yuxin Yang [verfasserIn] Xiaolong Wang [verfasserIn] Yulin Chen [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2020 |
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Schlagwörter: |
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Übergeordnetes Werk: |
In: Cells - MDPI AG, 2012, 9(2020), 2, p 445 |
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Übergeordnetes Werk: |
volume:9 ; year:2020 ; number:2, p 445 |
Links: |
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DOI / URN: |
10.3390/cells9020445 |
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Katalog-ID: |
DOAJ017436001 |
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10.3390/cells9020445 doi (DE-627)DOAJ017436001 (DE-599)DOAJe290060e6d8c422ba874670c6b6de2bd DE-627 ger DE-627 rakwb eng QH301-705.5 Qiang Ding verfasserin aut Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier MicroRNAs play key roles during ovary development, with emerging evidence suggesting that miR-202-5p is specifically expressed in female animal gonads. Granulosa cells (GCs) are somatic cells that are closely related to the development of female gametes in mammalian ovaries. However, the biological roles of miR-202-5p in GCs remain unknown. Here, we show that miR-202-5p is specifically expressed in GCs and accumulates in extracellular vesicles (EVs) from large growth follicles in goat ovaries. In vitro assays showed that miR-202-5p induced apoptosis and suppressed the proliferation of goat GCs. We further revealed that miR-202-5p is a functional miRNA that targets the transforming growth factor-beta type II receptor (<i<TGFβR2</i<). MiR-202-5p attenuated TGF-β/SMAD signaling through the degradation of TGFβR2 at both the mRNA and protein level, decreasing p-SMAD3 levels in GCs. Moreover, we verified that steroidogenic factor 1 (SF1) is a transcriptional factor that binds to the promoters of miR-202 and cytochrome P450 family 19 subfamily A member 1 (<i<CYP19A1</i<) through luciferase reporter and chromatin immunoprecipitation (ChIP) assays. That contributed to positive correlation between miR-202-5p and <i<CYP19A1</i< expression and estradiol (E2) release. Furthermore, SF1 repressed TGFβR2 and p-SMAD3 levels in GCs through the transactivation of miR-202-5p. Taken together, these results suggest a mechanism by which miR-202-5p regulates canonical TGF-β/SMAD signaling through targeting <i<TGFβR2</i< in GCs. This provides insight into the transcriptional regulation of <i<miR-202</i< and <i<CYP19A1</i< during goat ovarian follicular development. mir-202-5p <i<tgfβr2</i< tgf-β signaling pathway goat granulosa cell cell apoptosis sf1 Biology (General) Miaohan Jin verfasserin aut Yaoyue Wang verfasserin aut Jiao Liu verfasserin aut Peter Kalds verfasserin aut Ying Wang verfasserin aut Yuxin Yang verfasserin aut Xiaolong Wang verfasserin aut Yulin Chen verfasserin aut In Cells MDPI AG, 2012 9(2020), 2, p 445 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:9 year:2020 number:2, p 445 https://doi.org/10.3390/cells9020445 kostenfrei https://doaj.org/article/e290060e6d8c422ba874670c6b6de2bd kostenfrei https://www.mdpi.com/2073-4409/9/2/445 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 2, p 445 |
spelling |
10.3390/cells9020445 doi (DE-627)DOAJ017436001 (DE-599)DOAJe290060e6d8c422ba874670c6b6de2bd DE-627 ger DE-627 rakwb eng QH301-705.5 Qiang Ding verfasserin aut Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier MicroRNAs play key roles during ovary development, with emerging evidence suggesting that miR-202-5p is specifically expressed in female animal gonads. Granulosa cells (GCs) are somatic cells that are closely related to the development of female gametes in mammalian ovaries. However, the biological roles of miR-202-5p in GCs remain unknown. Here, we show that miR-202-5p is specifically expressed in GCs and accumulates in extracellular vesicles (EVs) from large growth follicles in goat ovaries. In vitro assays showed that miR-202-5p induced apoptosis and suppressed the proliferation of goat GCs. We further revealed that miR-202-5p is a functional miRNA that targets the transforming growth factor-beta type II receptor (<i<TGFβR2</i<). MiR-202-5p attenuated TGF-β/SMAD signaling through the degradation of TGFβR2 at both the mRNA and protein level, decreasing p-SMAD3 levels in GCs. Moreover, we verified that steroidogenic factor 1 (SF1) is a transcriptional factor that binds to the promoters of miR-202 and cytochrome P450 family 19 subfamily A member 1 (<i<CYP19A1</i<) through luciferase reporter and chromatin immunoprecipitation (ChIP) assays. That contributed to positive correlation between miR-202-5p and <i<CYP19A1</i< expression and estradiol (E2) release. Furthermore, SF1 repressed TGFβR2 and p-SMAD3 levels in GCs through the transactivation of miR-202-5p. Taken together, these results suggest a mechanism by which miR-202-5p regulates canonical TGF-β/SMAD signaling through targeting <i<TGFβR2</i< in GCs. This provides insight into the transcriptional regulation of <i<miR-202</i< and <i<CYP19A1</i< during goat ovarian follicular development. mir-202-5p <i<tgfβr2</i< tgf-β signaling pathway goat granulosa cell cell apoptosis sf1 Biology (General) Miaohan Jin verfasserin aut Yaoyue Wang verfasserin aut Jiao Liu verfasserin aut Peter Kalds verfasserin aut Ying Wang verfasserin aut Yuxin Yang verfasserin aut Xiaolong Wang verfasserin aut Yulin Chen verfasserin aut In Cells MDPI AG, 2012 9(2020), 2, p 445 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:9 year:2020 number:2, p 445 https://doi.org/10.3390/cells9020445 kostenfrei https://doaj.org/article/e290060e6d8c422ba874670c6b6de2bd kostenfrei https://www.mdpi.com/2073-4409/9/2/445 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 2, p 445 |
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10.3390/cells9020445 doi (DE-627)DOAJ017436001 (DE-599)DOAJe290060e6d8c422ba874670c6b6de2bd DE-627 ger DE-627 rakwb eng QH301-705.5 Qiang Ding verfasserin aut Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier MicroRNAs play key roles during ovary development, with emerging evidence suggesting that miR-202-5p is specifically expressed in female animal gonads. Granulosa cells (GCs) are somatic cells that are closely related to the development of female gametes in mammalian ovaries. However, the biological roles of miR-202-5p in GCs remain unknown. Here, we show that miR-202-5p is specifically expressed in GCs and accumulates in extracellular vesicles (EVs) from large growth follicles in goat ovaries. In vitro assays showed that miR-202-5p induced apoptosis and suppressed the proliferation of goat GCs. We further revealed that miR-202-5p is a functional miRNA that targets the transforming growth factor-beta type II receptor (<i<TGFβR2</i<). MiR-202-5p attenuated TGF-β/SMAD signaling through the degradation of TGFβR2 at both the mRNA and protein level, decreasing p-SMAD3 levels in GCs. Moreover, we verified that steroidogenic factor 1 (SF1) is a transcriptional factor that binds to the promoters of miR-202 and cytochrome P450 family 19 subfamily A member 1 (<i<CYP19A1</i<) through luciferase reporter and chromatin immunoprecipitation (ChIP) assays. That contributed to positive correlation between miR-202-5p and <i<CYP19A1</i< expression and estradiol (E2) release. Furthermore, SF1 repressed TGFβR2 and p-SMAD3 levels in GCs through the transactivation of miR-202-5p. Taken together, these results suggest a mechanism by which miR-202-5p regulates canonical TGF-β/SMAD signaling through targeting <i<TGFβR2</i< in GCs. This provides insight into the transcriptional regulation of <i<miR-202</i< and <i<CYP19A1</i< during goat ovarian follicular development. mir-202-5p <i<tgfβr2</i< tgf-β signaling pathway goat granulosa cell cell apoptosis sf1 Biology (General) Miaohan Jin verfasserin aut Yaoyue Wang verfasserin aut Jiao Liu verfasserin aut Peter Kalds verfasserin aut Ying Wang verfasserin aut Yuxin Yang verfasserin aut Xiaolong Wang verfasserin aut Yulin Chen verfasserin aut In Cells MDPI AG, 2012 9(2020), 2, p 445 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:9 year:2020 number:2, p 445 https://doi.org/10.3390/cells9020445 kostenfrei https://doaj.org/article/e290060e6d8c422ba874670c6b6de2bd kostenfrei https://www.mdpi.com/2073-4409/9/2/445 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 2, p 445 |
allfieldsGer |
10.3390/cells9020445 doi (DE-627)DOAJ017436001 (DE-599)DOAJe290060e6d8c422ba874670c6b6de2bd DE-627 ger DE-627 rakwb eng QH301-705.5 Qiang Ding verfasserin aut Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier MicroRNAs play key roles during ovary development, with emerging evidence suggesting that miR-202-5p is specifically expressed in female animal gonads. Granulosa cells (GCs) are somatic cells that are closely related to the development of female gametes in mammalian ovaries. However, the biological roles of miR-202-5p in GCs remain unknown. Here, we show that miR-202-5p is specifically expressed in GCs and accumulates in extracellular vesicles (EVs) from large growth follicles in goat ovaries. In vitro assays showed that miR-202-5p induced apoptosis and suppressed the proliferation of goat GCs. We further revealed that miR-202-5p is a functional miRNA that targets the transforming growth factor-beta type II receptor (<i<TGFβR2</i<). MiR-202-5p attenuated TGF-β/SMAD signaling through the degradation of TGFβR2 at both the mRNA and protein level, decreasing p-SMAD3 levels in GCs. Moreover, we verified that steroidogenic factor 1 (SF1) is a transcriptional factor that binds to the promoters of miR-202 and cytochrome P450 family 19 subfamily A member 1 (<i<CYP19A1</i<) through luciferase reporter and chromatin immunoprecipitation (ChIP) assays. That contributed to positive correlation between miR-202-5p and <i<CYP19A1</i< expression and estradiol (E2) release. Furthermore, SF1 repressed TGFβR2 and p-SMAD3 levels in GCs through the transactivation of miR-202-5p. Taken together, these results suggest a mechanism by which miR-202-5p regulates canonical TGF-β/SMAD signaling through targeting <i<TGFβR2</i< in GCs. This provides insight into the transcriptional regulation of <i<miR-202</i< and <i<CYP19A1</i< during goat ovarian follicular development. mir-202-5p <i<tgfβr2</i< tgf-β signaling pathway goat granulosa cell cell apoptosis sf1 Biology (General) Miaohan Jin verfasserin aut Yaoyue Wang verfasserin aut Jiao Liu verfasserin aut Peter Kalds verfasserin aut Ying Wang verfasserin aut Yuxin Yang verfasserin aut Xiaolong Wang verfasserin aut Yulin Chen verfasserin aut In Cells MDPI AG, 2012 9(2020), 2, p 445 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:9 year:2020 number:2, p 445 https://doi.org/10.3390/cells9020445 kostenfrei https://doaj.org/article/e290060e6d8c422ba874670c6b6de2bd kostenfrei https://www.mdpi.com/2073-4409/9/2/445 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 2, p 445 |
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Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2 |
abstract |
MicroRNAs play key roles during ovary development, with emerging evidence suggesting that miR-202-5p is specifically expressed in female animal gonads. Granulosa cells (GCs) are somatic cells that are closely related to the development of female gametes in mammalian ovaries. However, the biological roles of miR-202-5p in GCs remain unknown. Here, we show that miR-202-5p is specifically expressed in GCs and accumulates in extracellular vesicles (EVs) from large growth follicles in goat ovaries. In vitro assays showed that miR-202-5p induced apoptosis and suppressed the proliferation of goat GCs. We further revealed that miR-202-5p is a functional miRNA that targets the transforming growth factor-beta type II receptor (<i<TGFβR2</i<). MiR-202-5p attenuated TGF-β/SMAD signaling through the degradation of TGFβR2 at both the mRNA and protein level, decreasing p-SMAD3 levels in GCs. Moreover, we verified that steroidogenic factor 1 (SF1) is a transcriptional factor that binds to the promoters of miR-202 and cytochrome P450 family 19 subfamily A member 1 (<i<CYP19A1</i<) through luciferase reporter and chromatin immunoprecipitation (ChIP) assays. That contributed to positive correlation between miR-202-5p and <i<CYP19A1</i< expression and estradiol (E2) release. Furthermore, SF1 repressed TGFβR2 and p-SMAD3 levels in GCs through the transactivation of miR-202-5p. Taken together, these results suggest a mechanism by which miR-202-5p regulates canonical TGF-β/SMAD signaling through targeting <i<TGFβR2</i< in GCs. This provides insight into the transcriptional regulation of <i<miR-202</i< and <i<CYP19A1</i< during goat ovarian follicular development. |
abstractGer |
MicroRNAs play key roles during ovary development, with emerging evidence suggesting that miR-202-5p is specifically expressed in female animal gonads. Granulosa cells (GCs) are somatic cells that are closely related to the development of female gametes in mammalian ovaries. However, the biological roles of miR-202-5p in GCs remain unknown. Here, we show that miR-202-5p is specifically expressed in GCs and accumulates in extracellular vesicles (EVs) from large growth follicles in goat ovaries. In vitro assays showed that miR-202-5p induced apoptosis and suppressed the proliferation of goat GCs. We further revealed that miR-202-5p is a functional miRNA that targets the transforming growth factor-beta type II receptor (<i<TGFβR2</i<). MiR-202-5p attenuated TGF-β/SMAD signaling through the degradation of TGFβR2 at both the mRNA and protein level, decreasing p-SMAD3 levels in GCs. Moreover, we verified that steroidogenic factor 1 (SF1) is a transcriptional factor that binds to the promoters of miR-202 and cytochrome P450 family 19 subfamily A member 1 (<i<CYP19A1</i<) through luciferase reporter and chromatin immunoprecipitation (ChIP) assays. That contributed to positive correlation between miR-202-5p and <i<CYP19A1</i< expression and estradiol (E2) release. Furthermore, SF1 repressed TGFβR2 and p-SMAD3 levels in GCs through the transactivation of miR-202-5p. Taken together, these results suggest a mechanism by which miR-202-5p regulates canonical TGF-β/SMAD signaling through targeting <i<TGFβR2</i< in GCs. This provides insight into the transcriptional regulation of <i<miR-202</i< and <i<CYP19A1</i< during goat ovarian follicular development. |
abstract_unstemmed |
MicroRNAs play key roles during ovary development, with emerging evidence suggesting that miR-202-5p is specifically expressed in female animal gonads. Granulosa cells (GCs) are somatic cells that are closely related to the development of female gametes in mammalian ovaries. However, the biological roles of miR-202-5p in GCs remain unknown. Here, we show that miR-202-5p is specifically expressed in GCs and accumulates in extracellular vesicles (EVs) from large growth follicles in goat ovaries. In vitro assays showed that miR-202-5p induced apoptosis and suppressed the proliferation of goat GCs. We further revealed that miR-202-5p is a functional miRNA that targets the transforming growth factor-beta type II receptor (<i<TGFβR2</i<). MiR-202-5p attenuated TGF-β/SMAD signaling through the degradation of TGFβR2 at both the mRNA and protein level, decreasing p-SMAD3 levels in GCs. Moreover, we verified that steroidogenic factor 1 (SF1) is a transcriptional factor that binds to the promoters of miR-202 and cytochrome P450 family 19 subfamily A member 1 (<i<CYP19A1</i<) through luciferase reporter and chromatin immunoprecipitation (ChIP) assays. That contributed to positive correlation between miR-202-5p and <i<CYP19A1</i< expression and estradiol (E2) release. Furthermore, SF1 repressed TGFβR2 and p-SMAD3 levels in GCs through the transactivation of miR-202-5p. Taken together, these results suggest a mechanism by which miR-202-5p regulates canonical TGF-β/SMAD signaling through targeting <i<TGFβR2</i< in GCs. This provides insight into the transcriptional regulation of <i<miR-202</i< and <i<CYP19A1</i< during goat ovarian follicular development. |
collection_details |
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container_issue |
2, p 445 |
title_short |
Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2 |
url |
https://doi.org/10.3390/cells9020445 https://doaj.org/article/e290060e6d8c422ba874670c6b6de2bd https://www.mdpi.com/2073-4409/9/2/445 https://doaj.org/toc/2073-4409 |
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author2 |
Miaohan Jin Yaoyue Wang Jiao Liu Peter Kalds Ying Wang Yuxin Yang Xiaolong Wang Yulin Chen |
author2Str |
Miaohan Jin Yaoyue Wang Jiao Liu Peter Kalds Ying Wang Yuxin Yang Xiaolong Wang Yulin Chen |
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doi_str |
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up_date |
2024-07-04T01:35:47.065Z |
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