Designing and Constructing a Novel Artificial Pathway for Malonic Acid Production Biologically
Malonic acid is used as a common component of many products and processes in the pharmaceutical and cosmetic industries. Here, we designed a novel artificial synthetic pathway of malonic acid, in which oxaloacetate, an intermediate of cytoplasmic reductive tricarboxylic acid (rTCA) pathway, is conve...
Ausführliche Beschreibung
Autor*in: |
Shuying Gu [verfasserIn] Zhen Zhao [verfasserIn] Yonghong Yao [verfasserIn] Jingen Li [verfasserIn] Chaoguang Tian [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2022 |
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Schlagwörter: |
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Übergeordnetes Werk: |
In: Frontiers in Bioengineering and Biotechnology - Frontiers Media S.A., 2014, 9(2022) |
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Übergeordnetes Werk: |
volume:9 ; year:2022 |
Links: |
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DOI / URN: |
10.3389/fbioe.2021.820507 |
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Katalog-ID: |
DOAJ017657415 |
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10.3389/fbioe.2021.820507 doi (DE-627)DOAJ017657415 (DE-599)DOAJ7afa54b181024be3ae3ce03a583063f7 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Shuying Gu verfasserin aut Designing and Constructing a Novel Artificial Pathway for Malonic Acid Production Biologically 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Malonic acid is used as a common component of many products and processes in the pharmaceutical and cosmetic industries. Here, we designed a novel artificial synthetic pathway of malonic acid, in which oxaloacetate, an intermediate of cytoplasmic reductive tricarboxylic acid (rTCA) pathway, is converted to malonic semialdehyde and then to malonic acid, sequentially catalyzed by a-keto decarboxylase and malonic semialdehyde dehydrogenase. After the systematic screening, we discovered the enzyme oxaloacetate decarboxylase Mdc, catalyzing the first step of the artificially designed pathway in vitro. Then, this synthetic pathway was functionally constructed in cellulolytic thermophilic fungus Myceliophthora thermophila. After enhancement of glucose uptake, the titer of malonic acid achieved 42.5 mg/L. This study presents a novel biological pathway for producing malonic acid from renewable resources in the future. malonic acid a-keto decarboxylase oxaloacetic acid metabolic engineering Myceliophthora thermophila Biotechnology Shuying Gu verfasserin aut Shuying Gu verfasserin aut Zhen Zhao verfasserin aut Zhen Zhao verfasserin aut Zhen Zhao verfasserin aut Yonghong Yao verfasserin aut Yonghong Yao verfasserin aut Jingen Li verfasserin aut Jingen Li verfasserin aut Chaoguang Tian verfasserin aut Chaoguang Tian verfasserin aut In Frontiers in Bioengineering and Biotechnology Frontiers Media S.A., 2014 9(2022) (DE-627)74950403X (DE-600)2719493-0 22964185 nnns volume:9 year:2022 https://doi.org/10.3389/fbioe.2021.820507 kostenfrei https://doaj.org/article/7afa54b181024be3ae3ce03a583063f7 kostenfrei https://www.frontiersin.org/articles/10.3389/fbioe.2021.820507/full kostenfrei https://doaj.org/toc/2296-4185 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2022 |
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10.3389/fbioe.2021.820507 doi (DE-627)DOAJ017657415 (DE-599)DOAJ7afa54b181024be3ae3ce03a583063f7 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Shuying Gu verfasserin aut Designing and Constructing a Novel Artificial Pathway for Malonic Acid Production Biologically 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Malonic acid is used as a common component of many products and processes in the pharmaceutical and cosmetic industries. Here, we designed a novel artificial synthetic pathway of malonic acid, in which oxaloacetate, an intermediate of cytoplasmic reductive tricarboxylic acid (rTCA) pathway, is converted to malonic semialdehyde and then to malonic acid, sequentially catalyzed by a-keto decarboxylase and malonic semialdehyde dehydrogenase. After the systematic screening, we discovered the enzyme oxaloacetate decarboxylase Mdc, catalyzing the first step of the artificially designed pathway in vitro. Then, this synthetic pathway was functionally constructed in cellulolytic thermophilic fungus Myceliophthora thermophila. After enhancement of glucose uptake, the titer of malonic acid achieved 42.5 mg/L. This study presents a novel biological pathway for producing malonic acid from renewable resources in the future. malonic acid a-keto decarboxylase oxaloacetic acid metabolic engineering Myceliophthora thermophila Biotechnology Shuying Gu verfasserin aut Shuying Gu verfasserin aut Zhen Zhao verfasserin aut Zhen Zhao verfasserin aut Zhen Zhao verfasserin aut Yonghong Yao verfasserin aut Yonghong Yao verfasserin aut Jingen Li verfasserin aut Jingen Li verfasserin aut Chaoguang Tian verfasserin aut Chaoguang Tian verfasserin aut In Frontiers in Bioengineering and Biotechnology Frontiers Media S.A., 2014 9(2022) (DE-627)74950403X (DE-600)2719493-0 22964185 nnns volume:9 year:2022 https://doi.org/10.3389/fbioe.2021.820507 kostenfrei https://doaj.org/article/7afa54b181024be3ae3ce03a583063f7 kostenfrei https://www.frontiersin.org/articles/10.3389/fbioe.2021.820507/full kostenfrei https://doaj.org/toc/2296-4185 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2022 |
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10.3389/fbioe.2021.820507 doi (DE-627)DOAJ017657415 (DE-599)DOAJ7afa54b181024be3ae3ce03a583063f7 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Shuying Gu verfasserin aut Designing and Constructing a Novel Artificial Pathway for Malonic Acid Production Biologically 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Malonic acid is used as a common component of many products and processes in the pharmaceutical and cosmetic industries. Here, we designed a novel artificial synthetic pathway of malonic acid, in which oxaloacetate, an intermediate of cytoplasmic reductive tricarboxylic acid (rTCA) pathway, is converted to malonic semialdehyde and then to malonic acid, sequentially catalyzed by a-keto decarboxylase and malonic semialdehyde dehydrogenase. After the systematic screening, we discovered the enzyme oxaloacetate decarboxylase Mdc, catalyzing the first step of the artificially designed pathway in vitro. Then, this synthetic pathway was functionally constructed in cellulolytic thermophilic fungus Myceliophthora thermophila. After enhancement of glucose uptake, the titer of malonic acid achieved 42.5 mg/L. This study presents a novel biological pathway for producing malonic acid from renewable resources in the future. malonic acid a-keto decarboxylase oxaloacetic acid metabolic engineering Myceliophthora thermophila Biotechnology Shuying Gu verfasserin aut Shuying Gu verfasserin aut Zhen Zhao verfasserin aut Zhen Zhao verfasserin aut Zhen Zhao verfasserin aut Yonghong Yao verfasserin aut Yonghong Yao verfasserin aut Jingen Li verfasserin aut Jingen Li verfasserin aut Chaoguang Tian verfasserin aut Chaoguang Tian verfasserin aut In Frontiers in Bioengineering and Biotechnology Frontiers Media S.A., 2014 9(2022) (DE-627)74950403X (DE-600)2719493-0 22964185 nnns volume:9 year:2022 https://doi.org/10.3389/fbioe.2021.820507 kostenfrei https://doaj.org/article/7afa54b181024be3ae3ce03a583063f7 kostenfrei https://www.frontiersin.org/articles/10.3389/fbioe.2021.820507/full kostenfrei https://doaj.org/toc/2296-4185 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2022 |
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10.3389/fbioe.2021.820507 doi (DE-627)DOAJ017657415 (DE-599)DOAJ7afa54b181024be3ae3ce03a583063f7 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Shuying Gu verfasserin aut Designing and Constructing a Novel Artificial Pathway for Malonic Acid Production Biologically 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Malonic acid is used as a common component of many products and processes in the pharmaceutical and cosmetic industries. Here, we designed a novel artificial synthetic pathway of malonic acid, in which oxaloacetate, an intermediate of cytoplasmic reductive tricarboxylic acid (rTCA) pathway, is converted to malonic semialdehyde and then to malonic acid, sequentially catalyzed by a-keto decarboxylase and malonic semialdehyde dehydrogenase. After the systematic screening, we discovered the enzyme oxaloacetate decarboxylase Mdc, catalyzing the first step of the artificially designed pathway in vitro. Then, this synthetic pathway was functionally constructed in cellulolytic thermophilic fungus Myceliophthora thermophila. After enhancement of glucose uptake, the titer of malonic acid achieved 42.5 mg/L. This study presents a novel biological pathway for producing malonic acid from renewable resources in the future. malonic acid a-keto decarboxylase oxaloacetic acid metabolic engineering Myceliophthora thermophila Biotechnology Shuying Gu verfasserin aut Shuying Gu verfasserin aut Zhen Zhao verfasserin aut Zhen Zhao verfasserin aut Zhen Zhao verfasserin aut Yonghong Yao verfasserin aut Yonghong Yao verfasserin aut Jingen Li verfasserin aut Jingen Li verfasserin aut Chaoguang Tian verfasserin aut Chaoguang Tian verfasserin aut In Frontiers in Bioengineering and Biotechnology Frontiers Media S.A., 2014 9(2022) (DE-627)74950403X (DE-600)2719493-0 22964185 nnns volume:9 year:2022 https://doi.org/10.3389/fbioe.2021.820507 kostenfrei https://doaj.org/article/7afa54b181024be3ae3ce03a583063f7 kostenfrei https://www.frontiersin.org/articles/10.3389/fbioe.2021.820507/full kostenfrei https://doaj.org/toc/2296-4185 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2022 |
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10.3389/fbioe.2021.820507 doi (DE-627)DOAJ017657415 (DE-599)DOAJ7afa54b181024be3ae3ce03a583063f7 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Shuying Gu verfasserin aut Designing and Constructing a Novel Artificial Pathway for Malonic Acid Production Biologically 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Malonic acid is used as a common component of many products and processes in the pharmaceutical and cosmetic industries. Here, we designed a novel artificial synthetic pathway of malonic acid, in which oxaloacetate, an intermediate of cytoplasmic reductive tricarboxylic acid (rTCA) pathway, is converted to malonic semialdehyde and then to malonic acid, sequentially catalyzed by a-keto decarboxylase and malonic semialdehyde dehydrogenase. After the systematic screening, we discovered the enzyme oxaloacetate decarboxylase Mdc, catalyzing the first step of the artificially designed pathway in vitro. Then, this synthetic pathway was functionally constructed in cellulolytic thermophilic fungus Myceliophthora thermophila. After enhancement of glucose uptake, the titer of malonic acid achieved 42.5 mg/L. This study presents a novel biological pathway for producing malonic acid from renewable resources in the future. malonic acid a-keto decarboxylase oxaloacetic acid metabolic engineering Myceliophthora thermophila Biotechnology Shuying Gu verfasserin aut Shuying Gu verfasserin aut Zhen Zhao verfasserin aut Zhen Zhao verfasserin aut Zhen Zhao verfasserin aut Yonghong Yao verfasserin aut Yonghong Yao verfasserin aut Jingen Li verfasserin aut Jingen Li verfasserin aut Chaoguang Tian verfasserin aut Chaoguang Tian verfasserin aut In Frontiers in Bioengineering and Biotechnology Frontiers Media S.A., 2014 9(2022) (DE-627)74950403X (DE-600)2719493-0 22964185 nnns volume:9 year:2022 https://doi.org/10.3389/fbioe.2021.820507 kostenfrei https://doaj.org/article/7afa54b181024be3ae3ce03a583063f7 kostenfrei https://www.frontiersin.org/articles/10.3389/fbioe.2021.820507/full kostenfrei https://doaj.org/toc/2296-4185 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2022 |
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Designing and Constructing a Novel Artificial Pathway for Malonic Acid Production Biologically |
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Malonic acid is used as a common component of many products and processes in the pharmaceutical and cosmetic industries. Here, we designed a novel artificial synthetic pathway of malonic acid, in which oxaloacetate, an intermediate of cytoplasmic reductive tricarboxylic acid (rTCA) pathway, is converted to malonic semialdehyde and then to malonic acid, sequentially catalyzed by a-keto decarboxylase and malonic semialdehyde dehydrogenase. After the systematic screening, we discovered the enzyme oxaloacetate decarboxylase Mdc, catalyzing the first step of the artificially designed pathway in vitro. Then, this synthetic pathway was functionally constructed in cellulolytic thermophilic fungus Myceliophthora thermophila. After enhancement of glucose uptake, the titer of malonic acid achieved 42.5 mg/L. This study presents a novel biological pathway for producing malonic acid from renewable resources in the future. |
abstractGer |
Malonic acid is used as a common component of many products and processes in the pharmaceutical and cosmetic industries. Here, we designed a novel artificial synthetic pathway of malonic acid, in which oxaloacetate, an intermediate of cytoplasmic reductive tricarboxylic acid (rTCA) pathway, is converted to malonic semialdehyde and then to malonic acid, sequentially catalyzed by a-keto decarboxylase and malonic semialdehyde dehydrogenase. After the systematic screening, we discovered the enzyme oxaloacetate decarboxylase Mdc, catalyzing the first step of the artificially designed pathway in vitro. Then, this synthetic pathway was functionally constructed in cellulolytic thermophilic fungus Myceliophthora thermophila. After enhancement of glucose uptake, the titer of malonic acid achieved 42.5 mg/L. This study presents a novel biological pathway for producing malonic acid from renewable resources in the future. |
abstract_unstemmed |
Malonic acid is used as a common component of many products and processes in the pharmaceutical and cosmetic industries. Here, we designed a novel artificial synthetic pathway of malonic acid, in which oxaloacetate, an intermediate of cytoplasmic reductive tricarboxylic acid (rTCA) pathway, is converted to malonic semialdehyde and then to malonic acid, sequentially catalyzed by a-keto decarboxylase and malonic semialdehyde dehydrogenase. After the systematic screening, we discovered the enzyme oxaloacetate decarboxylase Mdc, catalyzing the first step of the artificially designed pathway in vitro. Then, this synthetic pathway was functionally constructed in cellulolytic thermophilic fungus Myceliophthora thermophila. After enhancement of glucose uptake, the titer of malonic acid achieved 42.5 mg/L. This study presents a novel biological pathway for producing malonic acid from renewable resources in the future. |
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Designing and Constructing a Novel Artificial Pathway for Malonic Acid Production Biologically |
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